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4. Mitosis y Meiosis, dos procesos fundamentales para la continuidad celular

Author

Bellingeri, Romina Valeria, Picco, Natalia Yanina, Bozzo, Aida Andrea, Borghi, Damiana, Cots, Debora Soledad, Díaz, Tomás, Romanini, María Cristina, Flores Bracamonte, María Carolina, and Costamagna, Iris

Subjects
Ciencias Biológicas, purl.org/becyt/ford/1 [https], Biología Celular, Microbiología, MITOSIS, BIOLOGIA CELULAR, MEIOSIS, purl.org/becyt/ford/1.6 [https], CIENCIAS NATURALES Y EXACTAS
Abstract

Este trabajo fue realizado en el marco del Programa Nexos: articulación entre escuelas secundarias y la universidad, convocado por el área de los Centros Regionales de Planificación de la Educación Superior (CPRES) de la Secretaría de Políticas Universitarias (SPU) del Ministerio de Educación de la Nación; extendido desde Octubre de 2017 a Octubre de 2018. Implicó la participación conjunta del Ministerio de Educación de la Provincia de Córdoba y la Universidad Nacional de Río Cuarto, ambas instituciones a través de los profesores de escuelas de nivel secundario de Río Cuarto y su región y profesores de diferentes carreras de grado de esta universidad.El eje del Programa se centró en la articulación entendida como una tarea compartida entre los profesores de nivel secundario y de nivel universitario en torno a tres líneas de acción: a- Reconocimiento de las diferentes opciones institucionales y propuestas de formación de educación universitaria y estrategias de aproximación a la vida universitaria; b- Formación de vocaciones tempranas y c- Producción de material educativo/secuencias didácticas/estrategias de evaluación. El trabajo colaborativo e interdisciplinario de los grupos mixtos constituidos por profesores de ambos niveles y la formación, tanto de estudiantes como de docentes, constituyeron las constantes que acompañaron el desarrollo del Programa de manera sostenida. Fil: Bellingeri, Romina Valeria. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Radioisótopos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Picco, Natalia Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Radioisótopos; Argentina Fil: Bozzo, Aida Andrea. Universidad Nacional de Río Cuarto; Argentina Fil: Borghi, Damiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquímicas y Naturales. Departamento de Anatomia Animal. Laboratorio de Biología Celular y Embriologia; Argentina Fil: Cots, Debora Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquímicas y Naturales. Departamento de Anatomia Animal. Laboratorio de Biología Celular y Embriologia; Argentina Fil: Díaz, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquímicas y Naturales. Departamento de Anatomia Animal. Laboratorio de Biología Celular y Embriologia; Argentina Fil: Romanini, María Cristina. Universidad Nacional de Río Cuarto; Argentina Fil: Flores Bracamonte, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquímicas y Naturales. Departamento de Anatomia Animal. Laboratorio de Biología Celular y Embriologia; Argentina Fil: Costamagna, Iris. Universidad Nacional de Río Cuarto; Argentina

Published
2018

5. Prevalence of Cryptosporidium spp. and Giardia spp., spatial clustering and patterns of shedding in dairy calves from Córdoba, Argentina

Author

Tiranti, Karina, Larriestra, Alejandro, Vissio, Claudina, Picco, Natalia, Alustiza, Fabrisio, Degioanni, Americo, and Vivas, Adriana

Subjects
tipo do solo, soil type, Giardia, Cryptosporidium, bezerros leiteiros, dairy calves, clustering, aglomerados
Abstract

The objectives of this study were to estimate calf and herd prevalence of Cryptosporidium spp. and Giardia spp., the herd prevalence clustering, spatial distribution according to soil type and shedding patterns in dairy calves from Cordoba, Argentina. Six hundred twenty calves younger than 7 weeks of age from 43 dairy herds were sampled. Samples were processed with the formol-ether and modified Ziehl-Neelsen techniques. Univariate analysis and Kruskall-Wallis tests were used. Factors associated were subjected to multivariate analysis with calf shedding intensity as the response variable. Clustering of herd prevalence was assessed by a scan method, and spatial analysis was applied to explore the overlapping of high prevalence herds and soil type. Overall calf prevalence for Cryptosporidium spp. oocysts and Giardia spp. cysts were 19.35% (95% CI: 16.14; 22.54) and 34.50% (95% CI: 30.69; 38.34), respectively. Calves younger than two weeks of age were almost four times more likely to be infected with Cryptosporidium, in comparison to older ones (RR: 3.78, 95% CI: 2.27; 6.26). Giardia spp. shedding showed a similar age pattern (RR: 1.33, 95% CI: 1.02; 1.75). A primary cluster of high Cryptosporidium prevalence was found, and high prevalence herds were located in areas with poor drained soil. Os objetivos deste estudo foram determinar a prevalência de Cryptosporidium spp. e Giardia spp., a presença de aglomerados, a distribuição espacial de acordo com o tipo de solo e padrões de eliminação de cistos e oocistos em bezerros de propriedades leiteiras em Córdoba, Argentina. Amostras fecais foram colhidas de 620 bezerros com menos de sete semanas de idade, provenientes de 43 propriedades leiteiras e examinadas pelas técnicas de formol-éter e Ziehl-Neelsen modificada. Foram realizadas uma análise univariada e o teste de Kruskal-Wallis e, em seguida, uma análise multivariada com a intensidade de eliminação de cistos e oocistos, como um evento. A presença de aglomerados foi determinada com o método de varredura e a análise espacial foi realizada para explorar a sobreposição de rebanhos com alta prevalência e tipo de solo. A prevalência de Cryptosporidium spp. e Giardia spp. foi de 19,35% (IC 95%: 16,14; 22,54) e 34,50% (IC 95%: 30,69; 38,34), respectivamente. A probabilidade de infecção por Cryptosporidium spp. foi quase quatro vezes maior para bezerros com menos de 2 semanas em comparação com os bezerros mais velhos (RR: 3,78, IC 95% 2,27; 6,26). O mesmo padrão de infecção relacionada à idade foi observado para Giardia spp. (RR: 1,33, IC 95% 1,02; 1,75). Foi encontrado um aglomerado primário com alta prevalência de Cryptosporidium spp., e rebanhos com alta prevalência foram localizados em solos mal drenados.

Published
2011

6. Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina

Author

Picco, Natalia Y., primary, Alustiza, Fabrisio E., additional, Bellingeri, Romina V., additional, Grosso, María C., additional, Motta, Carlos E., additional, Larriestra, Alejandro J., additional, Vissio, Claudina, additional, Tiranti, Karina I., additional, Terzolo, Horacio R., additional, Moreira, Ana R., additional, and Vivas, Adriana B., additional

Published
2015
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10. In vitro toxicity evaluation of hydrogel-carbon nanotubes composites on intestinal cells.

Author

Bellingeri, Romina, Alustiza, Fabrisio, Picco, Natalia, Acevedo, Diego, Molina, Maria Alejandra, Rivero, Rebeca, Grosso, Carolina, Motta, Carlos, Barbero, Cesar, and Vivas, Adriana

Subjects
BIOCOMPATIBILITY, NANOCOMPOSITE materials, HYDROGELS, CARBON nanotubes, TRANSMISSION electron microscopy
Abstract

ABSTRACT Composite materials based on carbon nanotubes (CNT) and polymeric hydrogels have become the subject matter of major interest for use as carriers in drug delivery research. The aim of this study was to evaluate the in vitro cytotoxicity of the hydrogel-carbon nanotube-chitosan (hydrogel-CNT-CH) composites on intestinal cells. Oxidized CNT were wrapped with chitosan (CH), Fourier transform infrared (FT-IR) analysis suggest that oxidized CNT interact with CH. Transmission electron microscopy (TEM) images show a CH layer lying around CNT. Chitosan wrapped CNT were incorporated to poly (acrylamide-co-acrylic acid) hydrogels. Swelling behavior in buffers at different pH were evaluated and revealed a significantly lower swelling when it is exposed to a acid buffer solution (pH 2.2). Mechanical properties were evaluated by measurements of elasticity and the material with CNT showed better mechanical properties. The incorporation and liberation of Egg Yolk Immunoglobulin from hydrogel-CNT-CH were also assessed and it revealed an improved performance. To evaluate the effect of these nanocomposites on cellular redox balance, intestinal cells were exposed to hydrogel-CNT-CH composites and antioxidant enzymes were assessed. Cytotoxicity and apoptosis were also evaluated. Hydrogel-CNT-CH composites induce no oxidative stress and there were no evidence of cytotoxicity or cell death. These preliminary findings suggest that hydrogel-CNT-CH composites show improved properties and good biocompatibility in vitro making these biomaterials promising systems for drug delivery purposes. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41370. [ABSTRACT FROM AUTHOR]

Published
2015
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11. Molecular screening of pathogenic Escherichia colistrains isolated from dairy neonatal calves in Cordoba province, Argentina

Author

Picco, Natalia Y., Alustiza, Fabrisio E., Bellingeri, Romina V., Grosso, María C., Motta, Carlos E., Larriestra, Alejandro J., Vissio, Claudina, Tiranti, Karina I., Terzolo, Horacio R., Moreira, Ana R., and Vivas, Adriana B.

Abstract

The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia colistrains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7%) healthy and 225 (36.3%) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coliisolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. colivirulence profiles was spatially described in terms of spatial distribution. A total of 30.1% isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. colivirulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. colivirulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. colistrains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.

Published
2015
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12. Frequency of virulence genes of Escherichia coli among newborn piglets from an intensive pig farm in Argentina.

Author

Alustiza FE, Picco NY, Bellingeri RV, Terzolo HR, and Vivas AB

Subjects
Animals, Animals, Newborn, Argentina, Swine, Virulence, Escherichia coli genetics, Escherichia coli pathogenicity
Abstract

The enterotoxigenic and porcine enteropathogenic Escherichia coli (EtEc and PEPEc) strains are agents associated with swine neonatal diarrhea, causing economic losses in swine production. The main goal of this study was to identify virulence genes of EtEc, verotoxigenic (VtEc) and PEPEc in intestinal strains responsible for swine diseases, by molecular typing using Pcr in newborn piglets from an intensive farm system. Two hundred and sixty seven rectal swabbings from 7-15 days- old landrace x large White crossbred piglets were taken, and 123 randomly selected samples, biochemically compatible with E. coli, were tested for E. coli virulence genes by Pcr. A frequency (%) compatible with: 68 EtEc, 24 VtEc, and 8 EPEc were found. of all E. coli strains studied, 19.51 % carried at least one virulence gene. These data showed conclusively that, in spite of the application of strict sanitary measures in the intensive farm, genes encoding virulence factors of intestinal pathogens compatible with EtEc are still detected; therefore these strains will probably keep circulating among animals.

Published
2012

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