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39 results on '"Piccini, Daniele"'

2. The human nucleoporin Tpr protects cells from RNA-mediated replication stress

Author

Kosar, Martin, Giannattasio, Michele, Piccini, Daniele, Maya-Mendoza, Apolinar, García-Benítez, Francisco, Bartkova, Jirina, Barroso, Sonia I., Gaillard, Hélène, Martini, Emanuele, Restuccia, Umberto, Ramirez-Otero, Miguel Angel, Garre, Massimiliano, Verga, Eleonora, Andújar-Sánchez, Miguel, Maynard, Scott, Hodny, Zdenek, Costanzo, Vincenzo, Kumar, Amit, Bachi, Angela, Aguilera, Andrés, Bartek, Jiri, and Foiani, Marco

Published
2021
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7. The human nucleoporin Tpr protects cells from RNA-mediated replication stress

Author

Universidad de Sevilla. Departamento de Genética, Kosar, Martin, Giannattasio, Michele, Piccini, Daniele, Maya Mendoza, Apolinar, García Benítez, Francisco, Bartkova, Jirina, Barroso Ceballos, Sonia Inés, Gaillard, Hélène, Martini, Emanuele, Restuccia, Umberto, Ramírez Otero, Miguel Ángel, Garre, Massimiliano, Verga, Eleonora, Andújar Sánchez, Miguel, Maynard, Scott, Hodny, Zdenek, Costanzo, Vincenzo, Kumar, Amit, Bachi, Angela, Aguilera López, Andrés, Bartek, Jiri, Foiani, Marco, Universidad de Sevilla. Departamento de Genética, Kosar, Martin, Giannattasio, Michele, Piccini, Daniele, Maya Mendoza, Apolinar, García Benítez, Francisco, Bartkova, Jirina, Barroso Ceballos, Sonia Inés, Gaillard, Hélène, Martini, Emanuele, Restuccia, Umberto, Ramírez Otero, Miguel Ángel, Garre, Massimiliano, Verga, Eleonora, Andújar Sánchez, Miguel, Maynard, Scott, Hodny, Zdenek, Costanzo, Vincenzo, Kumar, Amit, Bachi, Angela, Aguilera López, Andrés, Bartek, Jiri, and Foiani, Marco

Abstract

Although human nucleoporin Tpr is frequently deregulated in cancer, its roles are poorly understood. Here we show that Tpr depletion generates transcription-dependent replication stress, DNA breaks, and genomic instability. DNA fiber assays and electron microscopy visualization of replication intermediates show that Tpr deficient cells exhibit slow and asymmetric replication forks under replication stress. Tpr deficiency evokes enhanced levels of DNA-RNA hybrids. Additionally, complementary proteomic strategies identify a network of Tpr-interacting proteins mediating RNA processing, such as MATR3 and SUGP2, and functional experiments confirm that their depletion trigger cellular phenotypes shared with Tpr deficiency. Mechanistic studies reveal the interplay of Tpr with GANP, a component of the TREX-2 complex. The Tpr-GANP interaction is supported by their shared protein level alterations in a cohort of ovarian carcinomas. Our results reveal links between nucleoporins, DNA transcription and replication, and the existence of a network physically connecting replication forks with transcription, splicing, and mRNA export machinery.

Published
2021

8. The human nucleoporin Tpr protects cells from RNA-mediated replication stress

Author

Associazione Italiana per la Ricerca sul Cancro, Danish Cancer Society Research Center, Danish National Research Foundation, Swedish Research Council, Lundbeck Foundation, Technology Agency of the Czech Republic, European Research Council, Ministerio de Economía y Competitividad (España), European Commission, Ministerio de Educación, Cultura y Deporte (España), Fondazione Umberto Veronesi, Kosar, Martin, Giannattasio, Michele, Piccini, Daniele, Maya-Mendoza, Apolinar, García-Benítez, Francisco, Bartkova, Jirina, Barroso, Sonia, Gaillard, Hélène, Martini, Emanuele, Restuccia, Umberto, Ramírez-Otero, Miguel Ángel, Garre, Massimiliano, Verga, Eleonora, Andújar-Sánchez, Miguel, Maynard, Scott, Hodny, Zdenek, Costanzo, Vicenzo, Kumar, Amit, Bachi, Angela, Aguilera, Andrés, Bartek, Jiri, Foiani, Marco, Associazione Italiana per la Ricerca sul Cancro, Danish Cancer Society Research Center, Danish National Research Foundation, Swedish Research Council, Lundbeck Foundation, Technology Agency of the Czech Republic, European Research Council, Ministerio de Economía y Competitividad (España), European Commission, Ministerio de Educación, Cultura y Deporte (España), Fondazione Umberto Veronesi, Kosar, Martin, Giannattasio, Michele, Piccini, Daniele, Maya-Mendoza, Apolinar, García-Benítez, Francisco, Bartkova, Jirina, Barroso, Sonia, Gaillard, Hélène, Martini, Emanuele, Restuccia, Umberto, Ramírez-Otero, Miguel Ángel, Garre, Massimiliano, Verga, Eleonora, Andújar-Sánchez, Miguel, Maynard, Scott, Hodny, Zdenek, Costanzo, Vicenzo, Kumar, Amit, Bachi, Angela, Aguilera, Andrés, Bartek, Jiri, and Foiani, Marco

Abstract

Although human nucleoporin Tpr is frequently deregulated in cancer, its roles are poorly understood. Here we show that Tpr depletion generates transcription-dependent replication stress, DNA breaks, and genomic instability. DNA fiber assays and electron microscopy visualization of replication intermediates show that Tpr deficient cells exhibit slow and asymmetric replication forks under replication stress. Tpr deficiency evokes enhanced levels of DNA-RNA hybrids. Additionally, complementary proteomic strategies identify a network of Tpr-interacting proteins mediating RNA processing, such as MATR3 and SUGP2, and functional experiments confirm that their depletion trigger cellular phenotypes shared with Tpr deficiency. Mechanistic studies reveal the interplay of Tpr with GANP, a component of the TREX-2 complex. The Tpr-GANP interaction is supported by their shared protein level alterations in a cohort of ovarian carcinomas. Our results reveal links between nucleoporins, DNA transcription and replication, and the existence of a network physically connecting replication forks with transcription, splicing, and mRNA export machinery.

Published
2021

12. Prime considerazioni sulla Scala celeste di Bernardino Baldi (Milano, Biblioteca Ambrosiana, S.Q.+.I.31)

Author

Barbieri, Edoardo R., Giola, Marco, Piccini, Daniele, Carpentieri, Chiara Maria, carpentieri chiara maria (ORCID:0000-0002-6377-3217), Barbieri, Edoardo R., Giola, Marco, Piccini, Daniele, Carpentieri, Chiara Maria, and carpentieri chiara maria (ORCID:0000-0002-6377-3217)

Abstract

The essay presents "La scala celeste" by Bernardino Baldi, composed in 1592 but published only in 1847 by Tito Cicconi. Divided into degrees and dedicated to St. Francis, the text suggests to the reader twelve themes to meditate about to gain eternal salvation. Each chapter consists of a prose introduction and a song. First, the history and physical description of the autograph manuscript (Milan, Biblioteca Ambrosiana, S.Q.+.I.31) is given; then, the contents of the work are offered. In the second part of the study, the song related to the tenth degree ("Della meditazione dell'inferno") is published with a commentary regarding philological, retorical, and prosopographical aspects.

Published
2019

13. Tra lo stil de' moderni e 'l sermon prisco. Studi di allievi e amici offerti a Giuseppe Frasso

Author

Barbieri, Edoardo (ORCID:0000-0002-9808-0656), Giola Marco (ORCID:0000-0003-3758-9499), Piccini, Daniele, Barbieri, Edoardo Roberto, Giola, Marco, Barbieri, Edoardo (ORCID:0000-0002-9808-0656), Giola Marco (ORCID:0000-0003-3758-9499), Piccini, Daniele, Barbieri, Edoardo Roberto, and Giola, Marco

Abstract

Il volume raccoglie una serie di contributi dedicati da colleghi e amici a Giuseppe Frasso per festeggiarlo. L'arco cronologico degli studi è ampio, dalle origini al Novecento. Altrettanto vari gli ambiti indagati: poesia e prosa, personalità decisive della storia letteraria e figure minori, ambienti eruditi, tradizioni testuali, libri manoscritti e libri a stampa. Il ventaglio delle ricerche dimostra con evidenza la ricchezza di un magistero generoso e aperto, mai concluso in se stesso e sempre pronto a confrontarsi con nuovi interessi. Unica costante è il rigore del metodo: la ricerca del vero (accertabile o ipotizzabile) attraverso una filologia integrale, intesa come paziente confronto con le fonti, i documenti, i testi.

Published
2019

19. Canto e disincanto. Primi appunti sul 'genio guastatori' e la poesia 'inclusiva'.

Author

Mussapi, Roberto, Ramat, Silvio, Oliva, Gianni, Pancheri, Alessandro, Baroni, Giorgio, Di Nardo, Martina, Papini, Maria Carla, Luzi, Alfredo, de Nobile, Sandro, Giannantonio, Valeria, Vitacolonna, Luciano, Verdino, Stefano, Luperini, Romano, Cadioli, Alberto, Piccini, Daniele, Bonifacino, Giuseppe, Giovannuzzi, Stefano, Gialloreto, Andrea, Dolfi, Anna, Crotti, Ilaria, Cimini, Mario, Del Gatto, Antonella, Pasquini, Luciana, Barsotti, Anna, Andreazza, Fabio, Di Nallo, Antonella., Langella, Giuseppe, Langella, Giuseppe (ORCID:0000-0003-4085-3107), Mussapi, Roberto, Ramat, Silvio, Oliva, Gianni, Pancheri, Alessandro, Baroni, Giorgio, Di Nardo, Martina, Papini, Maria Carla, Luzi, Alfredo, de Nobile, Sandro, Giannantonio, Valeria, Vitacolonna, Luciano, Verdino, Stefano, Luperini, Romano, Cadioli, Alberto, Piccini, Daniele, Bonifacino, Giuseppe, Giovannuzzi, Stefano, Gialloreto, Andrea, Dolfi, Anna, Crotti, Ilaria, Cimini, Mario, Del Gatto, Antonella, Pasquini, Luciana, Barsotti, Anna, Andreazza, Fabio, Di Nallo, Antonella., Langella, Giuseppe, and Langella, Giuseppe (ORCID:0000-0003-4085-3107)

Abstract

Si indagano le ragioni e gli esiti della crisi del genere lirico nel sistema letterario novecentesco, in una ricognizione ad ampio raggio che va dalla generazione del cosiddetto "genio guastatori" (crepuscolari, futuristi, anarchici, vociani) fino a Luzi e Caproni.

Published
2015

29. The Life Span Determinant p66Shc Localizes to Mitochondria Where It Associates with Mitochondrial Heat Shock Protein 70 and Regulates Trans-membrane Potential

Author

Orsini, Francesca, primary, Migliaccio, Enrica, additional, Moroni, Maurizio, additional, Contursi, Cristina, additional, Raker, Veronica A., additional, Piccini, Daniele, additional, Martin-Padura, Ines, additional, Pelliccia, Giovanni, additional, Trinei, Mirella, additional, Bono, Maria, additional, Puri, Claudia, additional, Tacchetti, Carlo, additional, Ferrini, Monica, additional, Mannucci, Roberta, additional, Nicoletti, Ildo, additional, Lanfrancone, Luisa, additional, Giorgio, Marco, additional, and Pelicci, Pier Giuseppe, additional

Published
2004
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39. Enrichment of DNA replication intermediates by EdU pull down.

Author

Pessina F, Romussi A, Piccini D, Mazzucco G, Varasi M, and Doksani Y

Subjects
DNA Replication, Biotin chemistry, DNA genetics
Abstract

Analysis of replication fork structures in electron microscopy (EM) can provide important mechanistic insights in DNA replication studies. A major challenge in this type of analysis is the paucity of replication intermediates. At any given time only a small fraction of the restriction fragments of genomic DNA will contain a replication fork. To address this issue, we have developed an EdU-pull-down procedure to enrich for replicating DNA. Cells are exposed to a brief pulse of EdU, a cleavable biotin moiety is attached to EdU by copper-catalyzed azide-alkyne cycloaddition (CuAAC), in conditions that minimize the damage to DNA. Biotinylated DNA is purified with streptavidin beads, in conditions that facilitate association of long DNA filaments. Finally, the DNA is eluted by cleaving the biotin moiety. This approach can enrich over 150-times for replicating DNA and about 50-times in replication fork structures, as verified by EM. This procedure could benefit analysis of replication intermediates in EM as well as other techniques for the study of replicating DNA., (Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.)

Published
2024
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