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Abstract There is need for advancing minimally invasive diagnostic techniques for sex differentiation at early life-stage classes in sea turtles. The objectives of this study were to determine whether there are sex-specific effects on the metabolome of the loggerhead sea turtle (Caretta caretta) and to identify potential biomarkers for sex classification at early life-stage classes (50 post-hatchling and 50 juvenile). Comprehensive analyses including plasma chemistry (n = 100) and untargeted (n = 48) metabolomic analyses were performed. Unlike with plasma chemistry analytes, there were significant differences in the metabolomic profiles between sexes. Nine metabolites differed significantly between sexes, with several metabolites deserving greater attention as potential biomarkers: C22H32O6 and pantothenic acid was higher in females than in males, and carboxylic acid ester (C10H16O5), 1-methylhistidine and C7H14N2O7 was higher in males than females. These differences remained constant both across and within the same life-stage class. This work identified a discernible effect on the metabolic profile based on sex of loggerhead sea turtles and it suggests that these metabolites and/or their ratios could serve as potential biomarker candidates for sex classification in this species.

Together with the gradual spread of BIM in the AECO industry, the concept of open BIM began to take root. Semantically HBIM has demonstrated its ability to dialogue with a wide range of stakeholders though multiple visualization and representation tools.But to be able to apply them from the perspective of interoperability among all the professionals, it is necessary to work with standards. For this reason, there has been a growing interest in HBIM, but the process requires specialized standards, today there are only a few recommendations.The bSDD allows linking between all the content inside the database. It provides a standardised workflow to guarantee data quality and information consistency. Projects must be based on openBIM methodologies, using only open, non-proprietary standards and formats, to guarantee an interoperable and collaborative workflow.It has been applied to the Rame Tower, a pre-16th century cultural heritage site, and a characterisation has been achieved based on an international standard language that is interoperable, allowing all stakeholders to share and exchange information about the objects and their properties, increasing the future possibilities for coordination and collaboration throughout the life cycle of the asset.The Digital Twin obtained through this HBIM modelling process is fully dynamic, sharable, and usable in all phases of the asset’s life cycle, and the characterisation and codification based on the interrelation between IFC and bSDD makes the project fully open and sharable.In addition the incorporation of blockchain technology together with HBIM helps to improve aspects such as: authorship and traceability of processes, metadata registration and quality in the creation phases among others.DOI: https://doi.org/10.20365/disegnarecon.32.2024.16

Abstract Background Neural stem and progenitor cells (NSPCs) from extra-neural origin represent a valuable tool for autologous cell therapy and research in neurogenesis. Identification of proneurogenic biomolecules on NSPCs would improve the success of cell therapies for neurodegenerative diseases. Preliminary data suggested that follicle-stimulating hormone (FSH) might act in this fashion. This study was aimed to elucidate whether FSH promotes development, self-renewal, and is proneurogenic on neurospheres (NS) derived from sheep ovarian cortical cells (OCCs). Two culture strategies were carried out: (a) long-term, 21-days NS culture (control vs. FSH group) with NS morphometric evaluation, gene expression analyses of stemness and lineage markers, and immunolocalization of NSPCs antigens; (b) NS assay to demonstrate FSH actions on self-renewal and differentiation capacity of NS cultured with one of three defined media: M1: positive control with EGF/FGF2; M2: control; and M3: M2 supplemented with FSH. Results In long-term cultures, FSH increased NS diameters with respect to control group (302.90 ± 25.20 μm vs. 183.20 ± 7.63 on day 9, respectively), upregulated nestin (days 15/21), Sox2 (day 21) and Pax6 (days 15/21) and increased the percentages of cells immunolocalizing these proteins. During NS assays, FSH stimulated NSCPs proliferation, and self-renewal, increasing NS diameters during the two expansion periods and the expression of the neuron precursor transcript DCX during the second one. In the FSH-group there were more frequent cell-bridges among neighbouring NS. Conclusions FSH is a proneurogenic hormone that promotes OCC-NSPCs self-renewal and NS development. Future studies will be necessary to support the proneurogenic actions of FSH and its potential use in basic and applied research related to cell therapy.

Abstract Background Global warming causes an increase in the levels of sugars in grapes and hence in ethanol after wine fermentation. Therefore, alcohol reduction is a major target in modern oenology. Deletion of the MKS1 gene, a negative regulator of the Retrograde Response pathway, in Saccharomyces cerevisiae was reported to increase glycerol and reduce ethanol and acetic acid in wine. This study aimed to obtain mutants with a phenotype similar to that of the MKS1 deletion strain by subjecting commercial S. cerevisiae wine strains to an adaptive laboratory evolution (ALE) experiment with the lysine toxic analogue S-(2-aminoethyl)-L-cysteine (AEC). Results In laboratory-scale wine fermentation, isolated AEC-resistant mutants overproduced glycerol and reduced acetic acid. In some cases, ethanol was also reduced. Whole-genome sequencing revealed point mutations in the Retrograde Response activator Rtg2 and in the homocitrate synthases Lys20 and Lys21. However, only mutations in Rtg2 were responsible for the overactivation of the Retrograde Response pathway and ethanol reduction during vinification. Finally, wine fermentation was scaled up in an experimental cellar for one evolved mutant to confirm laboratory-scale results, and any potential negative sensory impact was ruled out. Conclusions Overall, we have shown that hyperactivation of the Retrograde Response pathway by ALE with AEC is a valid approach for generating ready-to-use mutants with a desirable phenotype in winemaking.

Abstract Extreme hydrological and thermal regimes characterize the Mediterranean zone and can influence the phenology of greenhouse gas (GHG) emissions in reservoirs. Our study examined the seasonal changes in GHG emissions of a shallow, eutrophic, hardwater reservoir in Spain. We observed distinctive seasonal patterns for each gas. CH4 emissions substantially increased during stratification, influenced predominantly by the increase in water temperature, net ecosystem production, and the decline in reservoir mean depth. N2O emissions mirrored CH4's seasonal trend, significantly correlating to water temperature, wind speed, and gross primary production. Conversely, CO2 emissions decreased during stratification and displayed a quadratic, rather than a linear relationship with water temperature—an unexpected deviation from CH4 and N2O emission patterns—likely associated with photosynthetic uptake of bicarbonate and formation of intracellular calcite that might be exported to sediments. This investigation highlights the imperative of integrating these idiosyncratic patterns into GHG emissions models, enhancing their predictive power.

Abstract Donor-acceptor Stenhouse adducts (DASAs) are molecular photoswitches spurring wide interest because of their dynamic photophysical properties, complex photoswitching mechanism, and diverse applications. Despite breakthroughs in modularity for the donor, acceptor, and triene compartments, the backbone heteroatom remains static due to synthetic challenges. We provide a predictive tool and sought-after strategy to vary the heteroatom, introduce amino DASA photoswitches, and analyze backbone heteroatom effects on photophysical properties. Amino DASA synthesis is enabled by aza-Piancatelli rearrangements on pyrrole substrates, imparting an aromaticity-breaking rearrangement that capitalizes on nitrogen’s additional bonding orbital and the inductive properties of sulfonyl groups. Amino DASA structure is confirmed by single crystal X-ray diffraction, the photochromic properties are characterized, and the photoswitch isomerization is investigated. Overall, the discovered pyrrole rearrangement enables the study of the DASA backbone heteroatom compartment and furthers our insight into the structure-property relationship of this complex photoswitch.

Abstract Reservoirs act as carbon sinks when sedimentation of particulate organic carbon (POC) exceeds CO2 and CH4 emissions. Here, we study the poorly explored process where phytoplankton‐derived acidic polysaccharides (APs) aggregate into particulate organic matter, promoting carbon export to sediments. This source of POC in sediments can mineralize to CO2 and CH4 over various timescales. Our research, centered on a Mediterranean reservoir, elucidates phenological trends of APs and POC sedimentation and identifies their predominant drivers. Our findings present synchronic sedimentation patterns of POC and APs but identify a 2‐week delay between POC sedimentation and CH4 emissions. Despite its eutrophic status, our data demonstrate this reservoir's role as a carbon sink, sequestering 4.33 g C m−2 yr−1. This highlights the need to consider various time scales when quantifying carbon budgets in reservoirs.

La intervención en el patrimonio arquitectónico precisa un estudio previo global que abarque tanto su desarrollo histórico como sus características morfológicas y constructivas, así como su relación con el entorno en que se encuentra. En este trabajo se presenta la metodología empleada para la intervención en la fortificación histórica en tapia de cal y canto de Belver de los Montes, Zamora. La interpretación de las características histórico-constructivas de los restos mediante análisis multidisciplinares permite plantear una forma coherente de abordar las obras de consolidación de un tramo del recinto, que se encuentra en un estado de conservación precario. La metodología desarrollada para este ejemplo concreto podría servir de referencia para futuras intervenciones en elementos de arquitectura fortificada de similares características.

Background: In recent decades, we have observed an increase in ambient temperature due to the effects of climate change. Objective: In this study, we aimed to determine whether there is a correlation between environmental temperature and urgent consultations of pediatric patients related to febrile processes. Research design and method: We conducted an observational, retrospective, single-center study in the pediatric ED of the Hospital Universitario La Paz, Madrid, Spain. We analyzed visits to the ED for febrile processes during the last heat waves in Madrid. Results: Between 2018 and 2022, seven heat waves were recorded in Madrid. A total of 3967 patients with fever as the main reason for consultation attended during this period. In two of the seven heat waves analyzed, febrile-related visits were higher during the heat alert. The first occurred between 6/11/2022 and 6/16/2022, with 345 febrile-related ED visits, 25.4% more than the previous days and 19.7% more than the following days. During this heat wave, we observed a positive correlation between the maximum temperature recorded and the number of visits to the ED for febrile processes (Rs=0.552; p = 0.018). During the heat wave between 09/07/2022 and 17/07/2022, 362 febrile-related ED visits were 3.1% more than the previous days and 24.9% more than the following days. Conclusion: During recent heat waves in our city, we registered an increase in febrile-related visits compared with non-heat alert periods.

Abstract Zero- to ultralow-field nuclear magnetic resonance (ZULF NMR) allows molecular structure elucidation via measurement of electron-mediated spin-spin J-couplings. This study examines zero-field J-spectra from molecules with quadrupolar nuclei, exemplified by solutions of various isotopologues of ammonium cations. The spectra reveal differences between various isotopologues upon extracting precise J-coupling values from pulse-acquire measurements. A primary isotope effect, $$\triangle J=\left({\gamma }_{{}^{14}{{{{{\rm{N}}}}}}}/{\gamma }_{{}^{15}{{{{{\rm{N}}}}}}}\right){J}_{{}^{15}{{{{{\rm{N}}}}}}{{{{{\rm{H}}}}}}}-{J}_{{}^{14}{{{{{\rm{N}}}}}}{{{{{\rm{H}}}}}}}\approx -58$$ △ J = γ 14 N / γ 15 N J 15 N H − J 14 N H ≈ − 58 mHz, is deduced by analysis of the proton-nitrogen J-coupling ratios. This study points toward further experiments with symmetric cations containing quadrupolar nuclei, promising applications in biomedicine, energy storage, and benchmarking quantum chemistry calculations.

Purpose – To understand how organizations, public or private, must increase their productivity perception (PP), independently of the sector. This article aims to analyze PP in the digital transformation (DT) process to determine how it is affected by technostress (TS) and work engagement (WE), two concepts that seem to be forces opposing PP. Design/methodology/approach – The authors use data from a questionnaire addressed to personnel in two organizations (public and private). The analysis applies partial least squares technique to the 505 valid responses obtained from these organizations. This analysis is based not on representativeness but on uniqueness. Findings – The results suggest a positive, significant relationship between DT and PP. This article integrates DT and its effects on aspects of people's health, PP and WE. The model thus includes interactions of technology with human elements. In both business and administrative environments, PP is key to optimizing resources and survival of organizations. Research limitations/implications – DT processes are different and complex because every organization is different. The authors recommend expanding this study to other sectors in both spheres, public and private. Aligning the objectives of the institutions for aid with DT is also quite complicated. Practical implications – This study contributes to improving participating organizations. It also provides government institutions with a clear foundation from which to encourage actions that promote the health and WE of their workforce without reducing productivity. In addition, this study adds novelty to the research line. Originality/value – The authors have deepened this line of research by developing fuller knowledge of the relationships among novel and necessary variables in organizations. The authors provide complementary, different and inspiring value in addressing this line of research.

Aims and objectivesThis systematic review aims to: (1) explore which tools have been used in Spanish to measure compassion; (2) know which of these tools could be used to assess compassion in healthcare settings from the perspective of patients; (3) evaluate the quality of these patient-reported measures in Spanish contexts; and (4) determine which of these instruments would be best suited to be used in healthcare settings.BackgroundCompassion has been recognized as a fundamental dimension of quality healthcare.MethodsSeveral scientific databases were consulted for relevant records published up to December 16th, 2021. In accordance with PRISMA guidelines, 64 studies were included.Results and conclusionswhile existing instruments, validated in Spanish, allow for the measurement of self-compassion or compassion to others, there are no valid and reliable measures currently available in Spanish to measure patient-reported compassion.Relevance to clinical practiceIn order to ensure and promote compassion in the health care context, it is essential to have a valid and reliable tool to measure this construct in a patient-informed way, and this is currently not possible in the Spanish-speaking context because of the lack of such an instrument in Spanish.

Using solar photovoltaic power sources has become a discussed topic in the construction and energy industry. The pressing need to reduce reliance on fossil fuels, increasing costs of traditional electricity generation, and affording photovoltaic modules has sparked a growing interest in solar photovoltaics. This study aims to optimis e the layout of solar photovoltaic systems to minimise environmental impact and building load, comparing the performing south-oriented panels with east-west-oriented panels. The comprehensive analysis performed hinges on a diverse array of determinative factors that demand thoughtful consideration before embarking upon implementing any photovoltaic installation. These salient factors, including but not limited to structural integrity, incorporating ballast for stability enhancement, integrating requisite electrical components, selecting solar panels, quantifying energy production capabilities, assessing carbon emissions, and discerning associated benefits, require thoughtful consideration before implementing any photovoltaic installation. The findings derived from this study underscore that, within the context of the given geographical location, the solar photovoltaic system configured with an East-West orientation represents the optimal choice to reduce both emissions and structural load. This study provides a scientific basis for the construction industry and the energy field and guides the future development of photovoltaic installation projects in a more economic and environmentally friendly direction.