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This paper proposes a Nash bargaining cooperative game model for a microgrid cluster system with double re-energy-load delay considering electricity, heat and gas multi-energy synergies. With the minimization of the operating cost of each microgrid as the objective function, a low-carbon operation model of a multi-energy complementary integrated energy microgrid considering fuzzy opportunity constraints is developed, an optimal operation mechanism including carbon quota and carbon trading is assessed, and a carbon capture system and an electricity-gas conversion device are added to the improved cogeneration unit model. Source-load uncertainty in microgrids is described in terms of new fuzzy parameters of new energy and undefined parameters of load demand. Each microgrid plays a second game with the marginal contribution rate and carbon trading cost rate as the bargaining power. The model is solved in a distributed manner using the ADMM-RGE algorithm. Finally, the simulation results show that the proposed multi-microgrid power-sharing way maximizes the benefits of microgrid alliances; the cooperative help of microgrid alliances is pretty distributed according to the size of each microgrid's energy contribution; carbon capture joint power-gas systems and energy sharing methods between microgrids can effectively reduce carbon emissions during microgrid operation.

Objectives: To evaluate whether trans-rectal spectral optical tomography of total hemoglobin concentration (HbT) can image longitudinal and lateral developments of a canine transmissible venereal tumor (TVT) in a canine prostate. Methods: A near-infrared (NIR) applicator was integrated with a trans-rectal ultrasound (TRUS) transducer to perform ultrasound (US)-coupled optical tomography of the canine prostate. Spectral detection at 785 and 830 nm enabled quantitation of HbT. Canine TVT cells were injected into the right lobe of a dog's prostate gland. Longitudinal imaging assessment of the post-injection prostate was performed by coupled US/NIR imaging over a 45-day duration. Results: By day 7, NIR indicated TVT infiltration in the noninjected left prostatic lobe with the gray-scale US indistinct. By day 31, both NIR and gray-scale US revealed more widespread TVT involvement in the left than in the right lobe, as well as an extensive TVT mass in the caudal aspect of the gland, of which the peak HbT increased 3-fold and the mass volume grew exponentially over the 45-day duration. Increased blood supply to the mass was also observed on Doppler US. Conclusions: TRUS-coupled spectral optical tomography enhances assessment of the laterality and progression of prostate tumor compared with using gray-scale and Doppler TRUS. [ABSTRACT FROM AUTHOR]

In near-eye displays (NEDs), issues such as weight, heat, and power consumption mean that the rendering and computing power is usually insufficient. Due to this limitation, algorithms need to be further improved for the rapid generation of holograms. In this paper, we propose two methods based on the characteristics of the human eye in NEDs to accelerate the generation of the pinhole-type holographic stereogram (HS). In the first method, we consider the relatively fixed position of the human eye in NEDs. The number of visible pixels from each elemental image is very small due to the limited pupil size of an observing eye, and the calculated amount can be dramatically reduced. In the second method, the foveated region rendering method is adopted to further enhance the calculation speed. When the two methods are adopted at the same time, the calculation speed can be increased dozens of times. Simulations demonstrate that the proposed method can obviously enhance the generation speed of a pinhole-type HS.

This study proposes a technique to implement see-through three-dimensional (3-D) display based on point light source integral imaging. This display consists of an edge-lit light guide plate, a polarizer array, and a transparent liquid crystal display (LCD) panel without rear polarizer. The combination of the light guide plate and the polarizer array act as a transparent polarized point light source array (PLSA), and reconstructs the 3-D image with the elemental image array loaded on the LCD panel. The background rays passing through the gaps in the polarized PLSA carry the object information and implement see-through property. The experimental system is constructed based on 1-D integral imaging to verify the proposed method. The see-through and superposition capabilities of the proposed method are demonstrated.

TPG(tiny portable graphic) is a new image compression technology based on the video part of AVS2 standard,whose compression efficiency is notably higher than traditional image formats like JPG,PNG and GIF.Theory and feature of TPG image compression technology were introduced.Then,the compression efficiency of TPG and traditional image formats was compared.Results show that TPG has overwhelming advantage.

Abstract Background Since 1950, Brucella melitensis has been the predominant strain associated with human brucellosis in China. In this study we investigated the genotypic characteristics of B. melitensis isolates from China using a multiple-locus variable-number tandem-repeat analysis (MLVA) and evaluated the utility of MLVA with regards to epidemiological trace-back investigation. Results A total of 105 B. melitensis strains isolated from throughout China were divided into 69 MLVA types using MLVA-16. Nei's genetic diversity indices for the various loci ranged between 0.00 - 0.84. 12 out 16 loci were the low diversity with values < 0.2 and the most discriminatory markers were bruce16 and bruce30 with a diversity index of > 0.75 and containing 8 and 7 alleles, respectively. Many isolates were single-locus or double-locus variants of closely related B. melitensis isolates from different regions, including the north and south of China. Using panel 1, the majority of strains (84/105) were genotype 42 clustering to the 'East Mediterranean' B. melitensis group. Chinese B. melitensis are classified in limited number of closely related genotypes showing variation mainly at the panel 2B loci. Conclusion The MLVA-16 assay can be useful to reveal the predominant genotypes and strain relatedness in endemic or non-endemic regions of brucellosis. However it is not suitable for biovar differentiation of B. melitensis. Genotype 42 is widely distributed throughout China during a long time. Bruce 16 and bruce 30 in panel 2B markers are most useful for typing Chinese isolates.

We generalize the comparison result 2007 on Hamilton-Jacobi equations to nonlinear parabolic equations, then by using Perron's method to study the existence and uniqueness of time almost periodic viscosity solutions of nonlinear parabolic equations under usual hypotheses.

Abstract We here investigate the existence and uniqueness of the nontrivial, nonnegative solutions of a nonlinear ordinary differential equation: satisfying a specific decay rate: with and . Here and . Such a solution arises naturally when we study a very singular self-similar solution for a degenerate parabolic equation with nonlinear convection term defined on the half line .

Covering 2016 up to the end of 2023 Alpinia is the largest genus of flowering plants in the ginger family, Zingiberaceae, and comprises about 500 species. Many Alpinia are commonly cultivated ornamental plants, and some are used as spices or traditional medicine to treat inflammation, hyperlipidemia, and cancers. However, only a few comprehensive reviews have been published on the phytochemistry and pharmacology of this genus, and the latest review was published in 2017. In this review, we provide an extensive coverage of the studies on Alpinia species reported from 2016 through 2023, including newly isolated compounds and potential biological effects. The present review article shows that Alpinia species have a wide spectrum of pharmacological activities, most due to the activities of diarylheptanoids, terpenoids, flavonoids, and phenolics.

What Is Already Known About This Topic?: Brucellosis and severe fever with thrombocytopenia syndrome (SFTS) are neglected zoonoses, attributable respectively to Brucella and the SFTS virus (SFTSV). While the incidence of these diseases has been rising, instances of co-infection remain uncommon., What Is Added by This Report?: This represents the first documented case of a rare coinfection involving Brucella and SFTSV. We carried out an epidemiological analysis of patients diagnosed with brucellosis and those with SFTS at Yidu Central Hospital of Weifang. Our findings demonstrate a temporal and spatial overlap among the affected individuals., What Are the Implications for Public Health Practice?: Our findings suggest that co-infections arising from the spatiotemporal overlap of Brucella and SFTSV are plausible, necessitating heightened awareness and enhanced diagnostic measures., Competing Interests: No conflicts of interest., (Copyright and License information: Editorial Office of CCDCW, Chinese Center for Disease Control and Prevention 2024.)

Perilla frutescens var. acuta (Lamiaceae) is widely used not only as an oil or a spice, but also as a traditional medicine to treat colds, coughs, fever, and indigestion. As an ongoing effort, luteolin-7- O -diglucuronide ( 1 ), apigenin-7- O -diglucuronide ( 2 ), and rosmarinic acid ( 3 ) isolated from P. frutescens var. acuta were investigated for their anti-adipogenic and thermogenic activities in 3T3-L1 cells. Compound 1 exhibited a strong inhibition against adipocyte differentiation by suppressing the expression of Pparg and Cebpa over 52.0% and 45.0%, respectively. Moreover, 2 inhibited the expression of those genes in a dose-dependent manner [ Pparg : 41.7% (5 µM), 62.0% (10 µM), and 81.6% (50 µM); Cebpa : 13.8% (5 µM), 18.4% (10 µM), and 37.2% (50 µM)]. On the other hand, the P. frutescens var. acuta water extract showed moderate thermogenic activities. Compounds 1 and 3 also induced thermogenesis in a dose-dependent manner by stimulating the mRNA expressions of Ucp1 , Pgc1a , and Prdm16 . Moreover, an LC-MS/MS chromatogram of the extract was acquired using UHPLC-MS 2 and it was analyzed by feature-based molecular networking (FBMN) and the Progenesis QI software (version 3.0). The chemical profiling of the extract demonstrated that flavonoids and their glycoside derivatives, including those isolated earlier as well as rosmarinic acid, are present in P. frutescens var. acuta .

Meat discoloration starts at the interface between the bright red oxymyoglobin layer and the interior deoxymyoglobin layer. Currently, limited tools are available to characterize myoglobin forms formed within the sub-surface of meat. The objective was to demonstrate a needle-probe based single-fiber reflectance (SfR) spectroscopy approach for characterizing sub-surface myoglobin forms of beef psoas major muscles during retail storage. A 400-μm fiber was placed in a 17-gauge needle, and the assembly was inserted into the muscle at five depths of 1 mm increment and 1 cm lateral shift. Metmyoglobin content increased at all depths during display and content at 1 mm was greater compared to that of 2 to 5 mm depth. The a* values decreased (P < 0.05) during retail display aligning with the sub-surface formation of metmyoglobin. In summary, the results suggest that needle-probe SfR spectroscopy can determine interior myoglobin forms and characterize meat discoloration., Competing Interests: Declaration of competing interest None., (Copyright © 2024. Published by Elsevier Ltd.)

Understanding scattering insensitiveness in diffuse reflectance spectroscopy (DRS) will be useful to enhancing the spectral specificity to absorption. In DRS based on center-illuminated-area-detection (CIAD), the scattering response can saturate as the relative strength of scattering with respect to the collection size, represented by a dimensionless reduced scattering, increases over a threshold. However, the formation of saturation versus the same range of dimensionless reduced scattering may differ between a fixed reduced scattering over an increasing collection size (case 1) and an increasing reduced scattering over a fixed collection size (case 2), due to the absorption. Part III demonstrates the differences of the scattering saturation as well as the effect of absorption on it in the CIAD geometry between the two cases while assessed over the same range of the dimensionless reduced scattering. A model allows predicting the absorption-dependent levels of saturation and the corner parameters of saturation transition. When assessed for the absorption coefficient to vary over [0.001,0.01,0.1,1] m m -1 , the model-predicted levels of saturation agree with MC results with ≤2.2 % error in both cases. In comparison, the model-predicted corner parameters of saturation show much different agreement with MC results in the two cases, suggesting that the saturation pattern is much better formed in one than in the other. Experiments conforming to the CIAD geometry support the discrepancy of the saturating patterns between the two cases.

Background: With the development of domestic animal husbandry, the spread of brucellosis has accelerated, and the scope of the epidemic has expanded. The timely and accurate diagnosis of human brucellosis continues to challenge clinicians in endemic areas. Droplet digital PCR (ddPCR) technology can quickly and accurately determine DNA load in samples, providing laboratory evidence for diagnosis, prognosis and management of brucellosis patients. In this study, a ddPCR method was established to accurately quantify Brucella DNA load in whole blood samples, and its diagnostic, prognostic, and therapeutic value for human brucellosis was evaluated., Methods: Annealing temperature, primers, and probe targeting the Brucella bcsp31 gene were optimised, and the sensitivity, specificity and repeatability of the ddPCR assay were assessed using 94 whole blood samples from 61 confirmed and 33 suspected cases. Results were compared with those of quantitative PCR (qPCR). Nine follow-up brucellosis patients were also analysed by the two methods after 2 and 6 months of treatment., Results: Optimal primer and probe concentrations were 800 nmol/L and 400 nmol/L, respectively, and the optimal annealing temperature was 55.3 °C. The ddPCR results showed that the limit of detection was 1.87 copies per reaction, with high repeatability. The positive rates for ddPCR and qPCR were 88.5% and 75.4% among 61 serum agglutination test (SAT) positive patients. In addition, 57.6% (19/33) of suspected sero-negative samples were positive by ddPCR, but only 36.3% (12/33) were positive by qPCR. Analysis of nine post-therapy follow-up brucellosis patients revealed that the Brucella DNA load in the whole blood samples decreased after 2 and 6 months of treatment, and was slightly increased following relapse and continuous exposure., Conclusion: The ddPCR assay showed good accuracy for whole blood samples, and could be a potential diagnostic and prognostic tool for detecting Brucella., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

This part proposes a model of time-dependent diffuse photon remission for the center-illuminated-area-detection (CIAD) geometry, by virtue of area integration of the radially resolved time-dependent diffuse photon remission formulated with the master-slave dual-source scheme demonstrated in Part I for steady-state measurements. The time-domain model is assessed against Monte Carlo (MC) simulations limiting to only the Heyney-Greenstein scattering phase function for CIAD of physical scales and medium properties relevant to single-fiber reflectance (SfR) and over a 2 ns duration, in compliance with the timespan of the only experimental report of SfR demonstrated with a 50 µm gradient index fiber. The time-domain model-MC assessments are carried out for an absorption coefficient ranging three orders of magnitude over [0.001,0.01,0.1,1] m m -1 at a fixed scattering, and a reduced scattering coefficient ranging three orders of magnitude over [0.01,0.1,1,10] m m -1 at a fixed absorption, among others. Photons of shorter and longer propagation times, relative to the diameter of the area of collection, respond differently to the scattering and absorption changes. Limited comparisons of MC between CIAD and a top-hat geometry as the idealization of SfR reveal that the time-domain photon remissions of the two geometries differ appreciably in only the early arriving photons.

Diffuse photon remission associated with the center-illuminated-area-detection (CIAD) geometry has been useful for non-contact sensing and may inform single-fiber reflectance (SfR). This series of work advances model approaches that help enrich the understanding and applicability of the photon remission by CIAD. The general approach is to derive the diffuse photon remission by the area integration of the radially resolved diffuse reflectance while limiting the analysis to a medium exhibiting only the Heyney-Greenstein (HG) scattering phase function. Part I assesses the steady-state photon remission in CIAD over a reduced scattering scaled diameter of μ s ' d a r e a ∈[0.5×10 -3 ,10 3 ] that covers the range extensively modeled for SfR. The corresponding radially resolved diffuse reflectance is obtained by concatenating an empirical expression for the semi-ballistic region near the point-of-illumination and a formula utilizing a master-slave dual-source scheme over the semi-diffusive to a diffusive regime while being constrained by an extrapolated zero-boundary condition. The terminal algebraic photon remission is examined against Monte Carlo simulations for an absorption coefficient over [0.001,1] m m -1 , a reduced scattering coefficient over [0.01,1000] m m -1 , a HG scattering anisotropy factor within [0.5,0.95], and a diameter of the area of collection ranging [50,1000]µ m . The algebraic model is also applied to phantom data acquired over a ∼2 c m non-contact CIAD configuration and with a 200 µm SfR probe. The model approach will be extended in a subsequent work towards the time-of-flight characteristics of CIAD.

Objective To investigate whether exosomes derived from gastric cancer cells can affect macrophages in tumor microenvironment through miR-151-3p. Methods The expression of miR-151-3p in tumor tissues of patients with gastric cancer and normal tissues was detected by real time quantitative PCR; Gastric cancer cells overexpressing miR-151-3p were constructed, and exosomes were isolated and identified. The expression of CD11b and CD163 markers on RAW264.7 cells co-incubated with exosomes were detected by flow cytometry, and the effects of exosome carrying miR-151-3p on tumor growth and tumor-associated macrophages were evaluated in mice transplanted tumor model. Results The results of real time quantitative PCR showed that the level of miR-151-3p in gastric tumor tissues was significantly higher than that in normal tissues, and the content of miR-151-3p in gastric juice of most patients after operation was lower than that before operation; The content of miR-151-3p in exosomes of tumor cells overexpressing miR-151-3p was also significantly higher than that of untransfected cells. Exosomes carrying miR-151-3p can induce phenotypic differentiation of M2 in co-incubation with RAW264.7 cells. Similarly, tumor transplantation model also showed that exosomes carrying miR-151-3p can induce tumor-associated macrophages to polarize to M2 and promote tumor growth. Conclusion miR-151-3p derived from gastric cancer exosomes can induce the polarization of M2 macrophages and promote the growth of gastric cancer. The treatment of miR-151-3p may destroy the tumor microenvironment of immunosuppression, which assists the anti-tumor immunotherapy.

Background: Brucellosis poses a serious threat to human and animal health, particularly in developing countries such as China. The Inner Mongolia Autonomous Region is one of the most severely brucellosis-endemic provinces in China. Currently, the host immune responses functioning to control Brucella infection and development remain poorly understood. The aim of this study is to further clarify the key immunity characteristics of diverse stages of brucellosis in Inner Mongolia., Methods: We collected a total of 733 blood samples from acute (n = 137), chronic (n = 316), inapparent (n = 35), recovery (n = 99), and healthy (n = 146) groups from the rural community of Inner Mongolia between 2014 and 2015. The proportions of CD4 + , CD8 + , Th1, Th2, and Th17 T cells in peripheral blood and the expression of TLR2 and TLR4 in lymphocytes, monocytes and granulocytes were examined using flow cytometry analysis. The differences among the five groups were compared using one-way ANOVA and the Kruskal-Wallis method, respectively., Results: Our results revealed that the proportions of CD4 + and CD8 + T cells were significantly different among the acute, chronic, recovery, and healthy control groups (P < 0.05), with lower proportions of CD4 + T cells and a higher proportion of CD8 + T cells in the acute, chronic, and recovery groups. The proportion of Th1 cells in the acute, chronic, and inapparent groups was higher than that in the healthy and recovery groups; however, there was no significant difference between patients and healthy individuals (P > 0.05). The proportion of Th2 lymphocytes was significantly higher in the acute and healthy groups than in the inapparent group (P < 0.05). The proportion of Th17 cells in the acute group was significantly higher than that in the healthy control, chronic, and inapparent groups (P < 0.05). Finally, the highest expression of TLR4 in lymphocytes, monocytes and granulocytes was observed in the recovery group, and this was followed by the acute, chronic, healthy control, and inapparent groups. There was a significant difference between the recovery group and the other groups, except for the acute group (P < 0.05). Moreover, a correlation in TLR4 expression was observed in lymphocytes, monocytes and granulocytes among the five groups (r > 0.5), except for the inapparent group between lymphocytes and granulocytes (r = 0.34)., Conclusions: Two key factors (CD8 + T cells and TLR4) in human immune profiles may closely correlate with the progression of brucellosis. The detailed function of TLR4 in the context of a greater number of cell types or tissues in human or animal brucellosis and in larger samples should be further explored in the future., (© 2022. The Author(s).)

Brucella spp. are facultative intracellular pathogens that can persistently colonize animal host cells and cause zoonotic brucellosis. Brucellosis affects public health and safety and even affects economic development. Our lab found that a Brucella strain isolated from Marmota himalayana exhibited amikacin resistance. To annotate and analyze the potential resistance genes in this strain, we utilized sequencing platforms in this study and cloned potential resistance genes. The findings showed that the isolated strain belonged to B. abortus biovar 1 and was similar to B. abortus 2308. The isolate had amikacin resistance genes encoding aminoglycoside 3'-phosphotransferase. Based on the results of genome analysis, the isolated strain may have obtained amikacin resistance genes from Salmonella spp. through Tn3 family transposons. Notably, this study establishes a foundation for further research on the resistance mechanism of Brucella spp. and provides data that may be useful for the prevention and control of drug-resistant Brucella strains., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Robust models for single-fiber reflectance (SFR) are relatively complex [Opt. Lett.45, 2078 (2020)OPLEDP0146-959210.1364/OL.385845] due to overlapping of the illumination and collection areas that entails probability weighting of the spatial integration of photon-remission. We demonstrate, via analytical means for limiting cases and Monte Carlo simulation of broader conditions, that diffuse photon-remission collected by single-fiber geometry may be scaled over the center-illuminated photon-remission. We specify for a medium revealing Henyey-Greenstein (HG) scattering anisotropy that the diffuse photon-remission from a sub-diffusive area of a top-hat illumination is ∼84.9 % of that collected over the same area when under a centered-illumination. This ratio remains consistent over a reduced-scattering fiber-size product of μ s ' d fib =[10 -5 ,10 0 ], for absorption varying 3 orders of magnitude. When applied to hemoglobin oxygenation changes induced in an aqueous phantom using a 200 µm single-fiber probe, the center-illumination-scaled model of SFR produced fitting results agreeing with reference measurements.

Zingiber cassumunar Roxb. (Zingiberaceae), is an important medicinal plant known as "Plai (Phlai)" in Thailand, "Bangle" in Indonesia, and "Bulei" in China. Traditionally, this plant has been used to treat inflammation, pain, and respiratory problems. The rhizomes are the primary part of the plant that has been used for medicinal purposes due to their constituents with therapeutic properties, including phenylbutenoids, curcuminoids, and essential oils. Since the 1970s, many studies have been conducted on the phytochemicals and bioactivities of Z. cassumunar to establish fundamental scientific evidence that supports its use in traditional medicine. The accumulated biological studies on the extracts, solvent fractions, and constituents of Z. cassumunar have described their diverse medicinal properties, including antioxidant, anti-inflammatory, anticancer, neuroprotective/neurotrophic, cosmeceutical, and antifungal/antimicrobial bioactivities. In this review, we summarize information on the phytochemicals of Z. cassumunar and the bioactivities of its extracts and constituents.

Alpinia oxyphylla Miquel (Zingiberaceae) has been reported to show antioxidant, anti-inflammatory, and neuroprotective effects. In this study, two new eudesmane sesquiterpenes, 7α-hydroperoxy eudesma-3,11-diene-2-one ( 1 ) and 7β-hydroperoxy eudesma-3,11-diene-2-one ( 2 ), and a new eremophilane sesquiterpene, 3α-hydroxynootkatone ( 3 ), were isolated from the MeOH extract of dried fruits of A. oxyphylla along with eleven known sesquiterpenes ( 4 - 14 ). The structures were elucidated by the analysis of 1D/2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and optical rotation data. Compounds ( 1 - 3 , 5 - 14 ) were evaluated for their protective effects against tert -butyl hydroperoxide (tBHP)-induced oxidative stress in adipose-derived mesenchymal stem cells (ADMSCs). As a result, treatment with isolated compounds, especially compounds 11 and 12, effectively reverted the damage of tBHP on ADMSCs in a dose-dependent manner. In particular, 11 and 12 at 50 µM improved the viability of tBHP-toxified ADMSCs by 1.69 ± 0.05-fold and 1.61 ± 0.03-fold, respectively.

The Kubelka-Munk (KM) theory of diffuse photon remission from opaque media is widely applied to quality-control processes. Recent works based on radiative transfer revealed that the KM function as the backbone parameter of the method may saturate at strong absorption to cause the KM approach to be unfit to predict the change of diffuse reflectance from the medium at strong absorption. We demonstrate by empirical means based on Monte Carlo results that diffuse photon remission from a strong-absorbing medium depends simply upon the absorption/scattering ratio when evaluated over a large area centered at the point of illumination differing in geometry from those convenient for the KM approach. Our empirical prediction gives ∼11 % mean errors of the diffuse photon remission from thick opaque medium having an absorption coefficient ranging 0.001 to up to 1000 times stronger than the reduced-scattering coefficient. A slight modification to the KM function in terms of the absorption weighting and absorption-scattering coupling for use within the KM approach also noticeably improves the prediction of diffuse photon remission from thick opaque medium of strong absorption. Our empirical model and the KM approach using the modified KM function were compared against measurements from a thick opaque medium, of which the absorption coefficient was changed over four orders of magnitude.

Fangchinoline (FAN), an alkaloid extracted from Stephania tetrandra, has a variety of biological and pharmacological activities, but evidence of its effects on colon adenocarcinoma (COAD) is limited. Therefore, the present study aimed to elucidate the molecular mechanisms by which FAN affects COAD. The cytotoxicity, viability and proliferation of DLD‑1 and LoVo cells were assessed in the presence of FAN using MTT and colony formation assays. The effects of FAN on apoptosis and the cell cycle in COAD cells were analysed by flow cytometry, and the migration and invasion of these cells were assessed by wound healing and Transwell experiments. Furthermore, a network pharmacological analysis was conducted to investigate the target of FAN and the results were confirmed by western blotting. In addition, a xenograft model was established in nude mice, and ultrasound imaging was used to assess the preclinical therapeutic effects of FAN in vivo. To the best of our knowledge, the results of this study provided the first evidence that FAN inhibited cellular proliferation, stemness, migration, invasion, angiogenesis and epithelial‑mesenchymal transition (EMT), and induced apoptosis and G1‑phase cell cycle arrest. Network pharmacological analysis further confirmed that FAN prevented EMT through the epidermal growth factor receptor (EGFR)‑phosphoinositide 3‑kinase (PI3K)/AKT signalling pathway. Finally, FAN significantly repressed tumour growth and promoted apoptosis in xenografts. Thus, targeting EGFR with FAN may offer a novel therapeutic approach for COAD.

Brucella spp. are facultative intracellular pathogens that can persistently colonize host cells and cause the zoonosis- brucellosis. The WHO recommended a treatment for brucellosis that involves a combination of doxycycline, rifampicin, or streptomycin. The aim of this study was to screen rifampicin-resistance related genes by transcriptomic analysis and gene recombination method at low rifampicin concentrations and to predict the major rifampicin- resistance pathways in Brucella spp. The results showed that the MIC value of rifampicin for B. melitensis bv.3 Ether was 0.5 μg / mL. Meanwhile, B. melitensis had an adaptive response to the resistance of low rifampicin in the early stages of growth, while the SNPs changed in the rpoB gene in the late stages of growth when incubated at 37°C with shaking. The transcriptome results of rifampicin induction showed that the functions of significant differentially expressed genes were focused on metabolic process, catalytic activity and membrane and membrane part. The VirB operon, β-resistance genes, ABC transporters, quorum-sensing genes, DNA repair- and replication -related genes were associated with rifampicin resistance when no variations of the in rpoB were detected. Among the VirB operons, VirB7-11 may play a central role in rifampicin resistance. This study provided new insights for screening rifampicin resistance-related genes and also provided basic data for the prevention and control of rifampicin-resistant Brucella isolates., Competing Interests: The authors have declared that no competing interests exist.