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Your search keyword '"Piao, Jiafang"' showing total 27 results
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27 results on '"Piao, Jiafang"'

26. Enhanced Fluorescence ELISA Based on HAT Triggering Fluorescence “Turn-on” with Enzyme–Antibody Dual Labeled AuNP Probes for Ultrasensitive Detection of AFP and HBsAg

Author

Wu, Yudong, Guo, Weisheng, Peng, Weipan, Zhao, Qian, Piao, Jiafang, Zhang, Bo, Wu, Xiaoli, Wang, Hanjie, Gong, Xiaoqun, and Chang, Jin

Abstract

At present, enzyme-linked immunosorbent assay (ELISA) is considered to be the most appropriate approach in clinical biomarker detection, with good specificity, low cost, and straightforward readout. However, unsatisfactory sensitivity severely hampers its wide application in clinical diagnosis. Herein, we designed a new kind of enhanced fluorescence enzyme-linked immunosorbent assay (FELISA) based on the human alpha-thrombin (HAT) triggering fluorescence “turn-on” signals. In this system, detection antibodies (Ab2) and HAT were labeled on the gold nanoparticles (AuNPs) to form the detection probes, and a bisamide derivative of Rhodamine110with fluorescence quenched served as the substrate of HAT. After the sandwich immunoreaction, HAT on the sandwich structure could catalyze the cleavage of the fluorescence-quenched substrate, leading to a strong fluorescence signal for sensing ultralow levels of alpha fetoprotein (AFP) and hepatitis B virus surface antigen (HBsAg). Under the optimized reaction conditions, AFP and HBsAg were detected at the ultralow concentrations of 10–8ng mL–1and 5 × 10–4IU mL–1, respectively, which were at least 104times lower than those of the conventional fluorescence assay and 106times lower than those of the conventional ELISA. In addition, we further discussed the efficiency of the sensitive FELISA in clinical serum samples, showing great potential in practical applications.

Published
2013
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27. Construction of Liposomes Mimicking Cell Membrane Structure through Frame-Guided Assembly.

Author

Wang C, Piao J, Li Y, Tian X, Dong Y, and Liu D

Subjects
Humans, Hydrophobic and Hydrophilic Interactions, Molecular Conformation, Cell Membrane Structures chemistry, Drug Delivery Systems methods, Liposomes chemistry
Abstract

The shape of eukaryotic cells is determined by the cytoskeleton associated with membrane proteins; however, the detailed mechanism of how the integral morphologies with structural stability is generated and maintained is still not fully understood. Here, based on the Frame-Guided Assembly (FGA) strategy, we successfully prepared hetero-liposomes with structural composition similar to that of eukaryotic cells by screening a series of transmembrane peptides as the leading hydrophobic groups (LHGs). It was demonstrated that the conformation and transmembrane mode of the LHGs played dominant roles during the FGA process. The FGA liposomes were formed with excellent stability, which may further provide evidence for the cytoskeleton-membrane protein-lipid bilayer model. Taking advantage of the biocompatibility and stability, the FGA liposomes were also applied to prepare novel drug delivery vehicles, which is promising in diagnostic imaging and cancer therapy applications., (© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2020
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