27 results on '"Piantoni C."'
Search Results
2. Sexual System, Sex Ratio, and Group Living in the Shrimp Thor amboinensis (De Man): Relevance to Resource-Monopolization and Sex-Allocation Theories
- Author
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BAEZA, J. A. and PIANTONI, C.
- Published
- 2010
3. Reproductive biology of the Amazon Lava Lizard (Tropidurus torquatus) from the Wet Chaco of Corrientes (Argentina): congeneric comparisons of ecotypic and interspecific variations
- Author
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Ortiz, M.A., Boretto, J.M., Piantoni, C., Alvarez, B.B., and Ibarguengoytia, N.R.
- Subjects
Sexual reproduction -- Research ,Lizards -- Genetic aspects -- Natural history ,Zoological research ,Zoology and wildlife conservation - Abstract
Herein we describe the reproductive biology of a population of the Amazon Lava Lizard (Tropidurus torquatus (Wied-Neuwied, 1820)) from Corrientes, northeastern Argentina (Wet Chaco). We describe the male and female reproductive cycles, minimum body size for adults, reproductive output, mean relative clutch mass, fat body cycles, and sexual dimorphism. Our results were compared with data on the reproductive biology of Brazilian populations of T. torquatus and congeneric species. In Corrientes, males of T. torquatus exhibited a continuous reproductive cycle, but with annual variation of testicular parameters associated with spermatogenic activity. In contrast, females were reproductive only from winter to summer (July- February), laying at least two clutches each of six eggs, on average, per reproductive season. The relative clutch mass and egg size values in Corrientes were the highest reported for the species. The annual cycle of energy storage (as fat bodies) was inversely correlated with reproductive activity in both sexes. Males differed from females in snout-vent length, head size, interlimb length, and tail length. We observed interpopulational differences in relative clutch mass, egg volume and mass, incubation period and hatching time, and the minimum body size for sexual maturity probably as a result of phenotypic plasticity or adaptation to local environmental conditions and likely both. Key words: reproductive cycle, Squamata, tropidurid, lizard, Tropidurus torquatus. Nous decrivons la biologie de la reproduction d'une population de lezards Tropidurus torquatus (Wied-Neuwied, 1820) de Corrientes, dans le nord-est de l'Argentine (Chaco humide). Nous decrivons les cycles de reproduction des males et des femelles, la taille minimum du corps des adultes, l'efficacite de la reproduction, la masse moyenne relative des pontes, les cycles des corps adipeux et le dimorphisme sexuel. Nos resultats sont compares a des donnees relatives a la biologie de la reproduction de populations bresiliennes de T. torquatus et d'especes congeneres. A Corrientes, les males de T. torquatus presentent un cycle de reproduction continu, mais des variations annuelles des parametres testiculaires associes a la spermatogenese. En revanche, les femelles ne sont reproductives que de l'hiver a l'ete (de juillet a fevrier), produisant aux moins deux pontes de six oeufs chacune en moyenne chaque saison de reproduction. Les valeurs de la masse relative des pontes et de la taille des oeufs a Corrientes etaient les plus importantes signalees pour l'espece. Le cycle annuel de stockage d'energie (sous forme de corps adipeux) etait inversement correle a l'activite de reproduction pour les deux sexes. Les males se distinguaient des femelles sur le plan de la longueur museau-cloaque, de la taille de la tete, de la longueur entre les membres et de la longueur de la queue. Nous avons observe des differences entre populations en ce qui concerne la masse relative des pontes, le volume et la masse des oeufs, la periode d'incubation et le moment de l'eclosion, ainsi que la taille minimum du corps a maturite sexuelle, ces differences etant probablement dues a la plasticite phenotypique, a l'adaptation a des conditions ambiantes locales ou, vraisemblablement, a une combinaison de ces deux phenomenes. [Traduit par la Redaction] Mots-cles : cycle de reproduction, squamates, tropidurides, lezard, Tropidurus torquatus., Introduction The timing of the reproductive cycles and the determination of the biotic and abiotic factors that affect them is critical in reproductive ecology (James and Shine 1985; Brown and [...]
- Published
- 2014
- Full Text
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4. Age-Related Changes in Cardiac Autonomic Modulation and Heart Rate Variability in Mice
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Piantoni C., Carnevali L., Molla D., Barbuti A., DiFrancesco D., Bucchi A., and Baruscotti M.
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aging ,arrhythmias (cardiac) ,autonomic cardiac modulation ,heart rate variability (HRV) ,mouse model - Abstract
Objective: The aim of this study was to assess age-related changes in cardiac autonomic modulation and heart rate variability (HRV) and their association with spontaneous and pharmacologically induced vulnerability to cardiac arrhythmias, to verify the translational relevance of mouse models for further in-depth evaluation of the link between autonomic changes and increased arrhythmic risk with advancing age. Methods: Heart rate (HR) and time- and frequency-domain indexes of HRV were calculated from Electrocardiogram (ECG) recordings in two groups of conscious mice of different ages (4 and 19 months old) (i) during daily undisturbed conditions, (ii) following peripheral ?-adrenergic (atenolol), muscarinic (methylscopolamine), and ?-adrenergic + muscarinic blockades, and (iii) following ?-adrenergic (isoprenaline) stimulation. Vulnerability to arrhythmias was evaluated during daily undisturbed conditions and following ?-adrenergic stimulation. Results: HRV analysis and HR responses to autonomic blockades revealed that 19-month-old mice had a lower vagal modulation of cardiac function compared with 4-month-old mice. This age-related autonomic effect was not reflected in changes in HR, since intrinsic HR was lower in 19-month-old compared with 4-month-old mice. Both time- and frequency-domain HRV indexes were reduced following muscarinic, but not ?-adrenergic blockade in younger mice, and to a lesser extent in older mice, suggesting that HRV is largely modulated by vagal tone in mice. Finally, 19-month-old mice showed a larger vulnerability to both spontaneous and isoprenaline-induced arrhythmias. Conclusion: The present study combines HRV analysis and selective pharmacological autonomic blockades to document an age-related impairment in cardiac vagal modulation in mice which is consistent with the human condition. Given their short life span, mice could be further exploited as an aged model for studying the trajectory of vagal decline with advancing age using HRV measures, and the mechanisms underlying its association with proarrhythmic remodeling of the senescent heart.
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- 2021
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5. Family-centred care for children and young people with cerebral palsy: results from an Italian multicenter observational study
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Molinaro, A, Fedrizzi, E, Calza, S, Pagliano, E, Galli, J, Fazzi, E, Trabacca, A, Vespino, T, Gallino, F, Villani, A, Picciolini, O, Cozzaglio, M, Baranello, G, Zambonin, F, Daniela, F, Mandalari, P, Mirabile, L, Nacinovich, R, Broggi, F, De Polo, G, Da Ruos, F, Cazzagon, M, Godio, M, Piantoni, C, Dusi, E, Marzaroli, M, Fusari, D, Arisi, D, Magri, S, Landi, N, Roberti, L, Signorini, S, Luparia, A, Sabbadini, M, Riccioli, N, Turconi, A, Molteni, F, Marcelli, M, Di Brina, C, Molinaro A., Fedrizzi E., Calza S., Pagliano E., Galli J., Fazzi E., Trabacca A., Vespino T., Gallino F., Villani A., Picciolini O., Cozzaglio M., Baranello G., Zambonin F., Daniela F., Mandalari P., Mirabile L., Nacinovich R., Broggi F., De Polo G., Da Ruos F., Cazzagon M., Godio M., Piantoni C., Dusi E., Marzaroli M., Fusari D., Arisi D., Magri S., Landi N., Roberti L., Signorini S., Luparia A., Sabbadini M., Riccioli N., Turconi A. C., Molteni F., Marcelli M., Di Brina C., Molinaro, A, Fedrizzi, E, Calza, S, Pagliano, E, Galli, J, Fazzi, E, Trabacca, A, Vespino, T, Gallino, F, Villani, A, Picciolini, O, Cozzaglio, M, Baranello, G, Zambonin, F, Daniela, F, Mandalari, P, Mirabile, L, Nacinovich, R, Broggi, F, De Polo, G, Da Ruos, F, Cazzagon, M, Godio, M, Piantoni, C, Dusi, E, Marzaroli, M, Fusari, D, Arisi, D, Magri, S, Landi, N, Roberti, L, Signorini, S, Luparia, A, Sabbadini, M, Riccioli, N, Turconi, A, Molteni, F, Marcelli, M, Di Brina, C, Molinaro A., Fedrizzi E., Calza S., Pagliano E., Galli J., Fazzi E., Trabacca A., Vespino T., Gallino F., Villani A., Picciolini O., Cozzaglio M., Baranello G., Zambonin F., Daniela F., Mandalari P., Mirabile L., Nacinovich R., Broggi F., De Polo G., Da Ruos F., Cazzagon M., Godio M., Piantoni C., Dusi E., Marzaroli M., Fusari D., Arisi D., Magri S., Landi N., Roberti L., Signorini S., Luparia A., Sabbadini M., Riccioli N., Turconi A. C., Molteni F., Marcelli M., and Di Brina C.
- Abstract
Background: Family-centred care (FCC) is recognized as the model of best practice for the provision of services for children who have physical disabilities and their families. Objective: To assess the overall perception of FCC provided in an Italian network of 17 rehabilitation services, as perceived by parents of children with cerebral palsy and professionals, and to explore whether children, families, service providers and service-related characteristics influence parent satisfaction regarding service provision in an FCC practice. Methods: The Measure of Processes of Care (MPOC-20) for parents/caregivers and the Measure of Processes of Care for Service Providers (MPOC-SP) for healthcare providers were used. For the purposes of the study, an ad hoc information form was developed to collect information concerning children, families, service providers and services. Results: A total of 382 parents/caregivers and 269 healthcare providers completed the MPOC questionnaires. Parents and service providers both identified the domains for enabling partnerships and interpersonal sensitivity as a strength, while the domain relating to general information was always scored the lowest. An advanced maternal age, being a single parent, being unemployed and having lower socio-economic status were factors identified as individually predictive of lower FCC scores on the MPOC-20. Higher intensity treatment, inpatient services, primary healthcare settings and settings identified with limited financial resources and reduced space/time for each family were other variables significantly associated with less favourable MPOC-20 ratings. Conclusions: The perception of FCC provided was fairly positive, with some areas of improvement, such as the domain of provision of information. Professionals should, therefore, provide better communication and take more time in giving information and attention to parents. Potential sources of variation in parent perceptions of FCC based on family characteri
- Published
- 2017
6. P1582Molecular basis of cardiac pacemaker ageing in mouse
- Author
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Atkinson, A., primary, Bucchi, A., additional, Piantoni, C., additional, Difrancesco, D., additional, Baruscotti, M., additional, Boyett, MR., additional, and Dobrzynski, H., additional
- Published
- 2017
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7. Vulnerability to climate warming of four genera of New World iguanians based on their thermal ecology
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Piantoni, C., primary, Navas, C. A., additional, and Ibargüengoytía, N. R., additional
- Published
- 2016
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8. Iotrochota revisited: a new sponge from Belize (Poecilosclerida, Iotrochotidae)
- Author
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Rützler, Klaus, Maldonado, Manuel, Piantoni, C., and Riesgo Gil, Ana
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Caribbean coral reefs ,Diversity ,Systematic revision ,Distributional ecology ,Sympatric distribution ,New species ,Porifera - Abstract
13 páginas, 7 figuras, 2 tablas., The systematics of tropical and subtropical western Atlantic species of Iotrochota is re-examined in light of the discovery of an undescribed species. Iotrochota birotulata (Higgin), the type species, is found to have more characters than previously recognised and is redefined with emphasis on a skeleton of spongin fibres containing stout, curved styles and strongyles (category I) and an interstitial spiculation consisting mainly of longer, slender and straight styles (II). Iotrochota bistylata Boury-Esnault is confirmed as a synonym of the above. The new species, named I. arenosa, sp. nov., differs in external morphology, strong mucus development, incorporation of sand and interstitial spicules that are mainly long, straight strongyles. Iotrochota atra (Whitfield), thought to be a synonym of I. birotulata, is recognised as a separate species occurring exclusively in the Bahamas and is found to be a senior synonym of I. imminuta Pulitzer-Finali; it is morphologically very similar to I. birotulata, but lacks birotulae and has a strongly reduced skeleton of megascleres (mostly one category of delicate strongyles). Iotrochota agglomerata Lehnert & van Soest is recognised as the fourth distinct species for its unusual colour (orange), thinly encrusting habit and special spiculation (styles with tylostylote modifications)., Some of the specimens we examined were provided by the USA Geological Survey’s Biological Resources Division, Minerals Management Service (contract no. 01ERGR0001).This study was partially supported by a grant of the Caribbean Coral Reef Ecosystems Program of the Smithsonian Institution (contribution no. 761) and a MEC grant of the Spanish government
- Published
- 2007
9. Reproductive biology of the Amazon Lava Lizard (Tropidurustorquatus) from the Wet Chaco of Corrientes (Argentina): congeneric comparisons of ecotypic and interspecific variations
- Author
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Ortiz, M.A., primary, Boretto, J.M., additional, Piantoni, C., additional, Álvarez, B.B., additional, and Ibargüengoytía, N.R., additional
- Published
- 2014
- Full Text
- View/download PDF
10. Sexual system, sex ratio, and group living in the shrimp Thor amboinensis (De Man): relevance to resource-monopolization and sex-allocation theories
- Author
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Juan Antonio Baeza and Piantoni, C.
11. Family-centred care for children and young people with cerebral palsy: results from an Italian multicenter observational study
- Author
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Molinaro, A., Fedrizzi, Ermellina, Calza, S., Pagliano, E., Galli, J., Fazzi, E., Trabacca, A., Vespino, T., Gallino, F., Villani, A., Picciolini, Odoardo, Cozzaglio, M., Baranello, G., Zambonin, F., Daniela, F., Mandalari, P., Mirabile, L., Nacinovich, R., Broggi, F., De Polo, G., Da Ruos, F., Cazzagon, M., Godio, M., Piantoni, Chiara, Dusi, E., Marzaroli, M., Fusari, Donatella, Arisi, D., Magri, Stefania, Landi, N., Roberti, L., Signorini, S., Luparia, A., Sabbadini, M., Riccioli, N., Turconi, ANNA CARLA, Molteni, Francesca, Marcelli, M., Di Brina, C., Molinaro, A, Fedrizzi, E, Calza, S, Pagliano, E, Galli, J, Fazzi, E, Trabacca, A, Vespino, T, Gallino, F, Villani, A, Picciolini, O, Cozzaglio, M, Baranello, G, Zambonin, F, Daniela, F, Mandalari, P, Mirabile, L, Nacinovich, R, Broggi, F, De Polo, G, Da Ruos, F, Cazzagon, M, Godio, M, Piantoni, C, Dusi, E, Marzaroli, M, Fusari, D, Arisi, D, Magri, S, Landi, N, Roberti, L, Signorini, S, Luparia, A, Sabbadini, M, Riccioli, N, Turconi, A, Molteni, F, Marcelli, M, and Di Brina, C
- Subjects
Male ,cerebral palsy ,family-centred care ,family-centred service ,MPOC-20 ,MPOC-SP ,service delivery ,Adolescent ,Caregivers ,Cerebral Palsy ,Child ,Child, Preschool ,Delivery of Health Care, Integrated ,Family ,Female ,Health Personnel ,Hospitalization ,Humans ,Infant ,Infant, Newborn ,Interpersonal Relations ,Italy ,Primary Health Care ,Young Adult ,Adolescent Health Services ,Child Health Services ,Integrated ,Preschool ,Newborn ,MED/39 - NEUROPSICHIATRIA INFANTILE ,Delivery of Health Care - Abstract
Background: Family-centred care (FCC) is recognized as the model of best practice for the provision of services for children who have physical disabilities and their families. Objective: To assess the overall perception of FCC provided in an Italian network of 17 rehabilitation services, as perceived by parents of children with cerebral palsy and professionals, and to explore whether children, families, service providers and service-related characteristics influence parent satisfaction regarding service provision in an FCC practice. Methods: The Measure of Processes of Care (MPOC-20) for parents/caregivers and the Measure of Processes of Care for Service Providers (MPOC-SP) for healthcare providers were used. For the purposes of the study, an ad hoc information form was developed to collect information concerning children, families, service providers and services. Results: A total of 382 parents/caregivers and 269 healthcare providers completed the MPOC questionnaires. Parents and service providers both identified the domains for enabling partnerships and interpersonal sensitivity as a strength, while the domain relating to general information was always scored the lowest. An advanced maternal age, being a single parent, being unemployed and having lower socio-economic status were factors identified as individually predictive of lower FCC scores on the MPOC-20. Higher intensity treatment, inpatient services, primary healthcare settings and settings identified with limited financial resources and reduced space/time for each family were other variables significantly associated with less favourable MPOC-20 ratings. Conclusions: The perception of FCC provided was fairly positive, with some areas of improvement, such as the domain of provision of information. Professionals should, therefore, provide better communication and take more time in giving information and attention to parents. Potential sources of variation in parent perceptions of FCC based on family characteristics and the organization of services highlight the importance the need to support services through the provision of greater financial and human resources.
- Published
- 2017
12. In-vitro studies of the NaV1.5 S805L Brugada mutation: The resting cell voltage is a critical element in determining the pathological or physiological phenotype of the current.
- Author
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Molla D, Frosio A, Bertoli G, Piantoni C, Arici M, Bartolucci C, Marchese P, Bazzini C, Barbuti A, Rocchetti M, Severi S, Bucchi A, and Baruscotti M
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- Humans, Action Potentials, Membrane Potentials, Animals, NAV1.5 Voltage-Gated Sodium Channel genetics, NAV1.5 Voltage-Gated Sodium Channel metabolism, Brugada Syndrome genetics, Brugada Syndrome physiopathology, Brugada Syndrome metabolism, Phenotype, Mutation
- Published
- 2024
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13. Pacemaker Channels and the Chronotropic Response in Health and Disease.
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Hennis K, Piantoni C, Biel M, Fenske S, and Wahl-Schott C
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- Humans, Animals, Biological Clocks, Heart Rate, Sinoatrial Node metabolism, Sinoatrial Node physiopathology, Sinoatrial Node physiology, Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels metabolism
- Abstract
Loss or dysregulation of the normally precise control of heart rate via the autonomic nervous system plays a critical role during the development and progression of cardiovascular disease-including ischemic heart disease, heart failure, and arrhythmias. While the clinical significance of regulating changes in heart rate, known as the chronotropic effect, is undeniable, the mechanisms controlling these changes remain not fully understood. Heart rate acceleration and deceleration are mediated by increasing or decreasing the spontaneous firing rate of pacemaker cells in the sinoatrial node. During the transition from rest to activity, sympathetic neurons stimulate these cells by activating β-adrenergic receptors and increasing intracellular cyclic adenosine monophosphate. The same signal transduction pathway is targeted by positive chronotropic drugs such as norepinephrine and dobutamine, which are used in the treatment of cardiogenic shock and severe heart failure. The cyclic adenosine monophosphate-sensitive hyperpolarization-activated current (I
f ) in pacemaker cells is passed by hyperpolarization-activated cyclic nucleotide-gated cation channels and is critical for generating the autonomous heartbeat. In addition, this current has been suggested to play a central role in the chronotropic effect. Recent studies demonstrate that cyclic adenosine monophosphate-dependent regulation of HCN4 (hyperpolarization-activated cyclic nucleotide-gated cation channel isoform 4) acts to stabilize the heart rate, particularly during rapid rate transitions induced by the autonomic nervous system. The mechanism is based on creating a balance between firing and recently discovered nonfiring pacemaker cells in the sinoatrial node. In this way, hyperpolarization-activated cyclic nucleotide-gated cation channels may protect the heart from sinoatrial node dysfunction, secondary arrhythmia of the atria, and potentially fatal tachyarrhythmia of the ventricles. Here, we review the latest findings on sinoatrial node automaticity and discuss the physiological and pathophysiological role of HCN pacemaker channels in the chronotropic response and beyond., Competing Interests: Disclosures None.- Published
- 2024
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14. E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation.
- Author
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Pruenster M, Immler R, Roth J, Kuchler T, Bromberger T, Napoli M, Nussbaumer K, Rohwedder I, Wackerbarth LM, Piantoni C, Hennis K, Fink D, Kallabis S, Schroll T, Masgrau-Alsina S, Budke A, Liu W, Vestweber D, Wahl-Schott C, Roth J, Meissner F, Moser M, Vogl T, Hornung V, Broz P, and Sperandio M
- Subjects
- Humans, Gasdermins, Neutrophils metabolism, E-Selectin metabolism, Calgranulin A metabolism, Calgranulin B metabolism, Inflammation metabolism, Inflammasomes metabolism, NLR Family, Pyrin Domain-Containing 3 Protein metabolism
- Abstract
S100A8/S100A9 is a proinflammatory mediator released by myeloid cells during many acute and chronic inflammatory disorders. However, the precise mechanism of its release from the cytosolic compartment of neutrophils is unclear. Here, we show that E-selectin-induced rapid S100A8/S100A9 release during inflammation occurs in an NLRP3 inflammasome-dependent fashion. Mechanistically, E-selectin engagement triggers Bruton's tyrosine kinase-dependent tyrosine phosphorylation of NLRP3. Concomitant potassium efflux via the voltage-gated potassium channel K
V 1.3 mediates ASC oligomerization. This is followed by caspase 1 cleavage and downstream activation of pore-forming gasdermin D, enabling cytosolic release of S100A8/S100A9. Strikingly, E-selectin-mediated gasdermin D pore formation does not result in cell death but is a transient process involving activation of the ESCRT III membrane repair machinery. These data clarify molecular mechanisms of controlled S100A8/S100A9 release from neutrophils and identify the NLRP3/gasdermin D axis as a rapid and reversible activation system in neutrophils during inflammation., (© 2023. The Author(s).)- Published
- 2023
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15. Lack of the transcription factor Nfix causes tachycardia in mice sinus node and rats neonatal cardiomyocytes.
- Author
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Landi S, Giannetti F, Benzoni P, Campostrini G, Rossi G, Piantoni C, Bertoli G, Bonfanti C, Carnevali L, Bucchi A, Baruscotti M, Careccia G, Messina G, and Barbuti A
- Abstract
Aims: Nfix is a transcription factor belonging to the Nuclear Factor I (NFI) family comprising four members (Nfia, b, c, x). Nfix plays important roles in the development and function of several organs. In muscle development, Nfix controls the switch from embryonic to fetal myogenesis by promoting fast twitching fibres. In the adult muscle, following injury, lack of Nfix impairs regeneration, inducing higher content of slow-twitching fibres. Nfix is expressed also in the heart, but its function has been never investigated before. We studied Nfix role in this organ., Methods: Using Nfix-null and wild type (WT) mice we analyzed: (1) the expression pattern of Nfix during development by qPCR and (2) the functional alterations caused by its absence, by in vivo telemetry and in vitro patch clamp analysis., Results and Conclusions: Nfix expression start in the heart from E12.5. Adult hearts of Nfix-null mice show a hearts morphology and sarcomeric proteins expression similar to WT. However, Nfix-null animals show tachycardia that derives form an intrinsic higher beating rate of the sinus node (SAN). Molecular and functional analysis revealed that sinoatrial cells of Nfix-null mice express a significantly larger L-type calcium current (Cacna1d + Cacna1c). Interestingly, downregulation of Nfix by sh-RNA in primary cultures of neonatal rat ventricular cardiomyocytes induced a similar increase in their spontaneous beating rate and in I
CaL current. In conclusion, our data provide the first demonstration of a role of Nfix that, increasing the L-type calcium current, modulates heart rate., (© 2023 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society.)- Published
- 2023
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16. Chinese natural compound decreases pacemaking of rabbit cardiac sinoatrial cells by targeting second messenger regulation of f-channels.
- Author
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Piantoni C, Paina M, Molla D, Liu S, Bertoli G, Jiang H, Wang Y, Wang Y, Wang Y, DiFrancesco D, Barbuti A, Bucchi A, and Baruscotti M
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- Action Potentials, Animals, China, Myocytes, Cardiac metabolism, Rabbits, Cyclic AMP metabolism, Second Messenger Systems
- Abstract
Tongmai Yangxin (TMYX) is a complex compound of the Traditional Chinese Medicine (TCM) used to treat several cardiac rhythm disorders; however, no information regarding its mechanism of action is available. In this study we provide a detailed characterization of the effects of TMYX on the electrical activity of pacemaker cells and unravel its mechanism of action. Single-cell electrophysiology revealed that TMYX elicits a reversible and dose-dependent (2/6 mg/ml) slowing of spontaneous action potentials rate (-20.8/-50.2%) by a selective reduction of the diastolic phase (-50.1/-76.0%). This action is mediated by a negative shift of the I
f activation curve (-6.7/-11.9 mV) and is caused by a reduction of the cyclic adenosine monophosphate (cAMP)-induced stimulation of pacemaker channels. We provide evidence that TMYX acts by directly antagonizing the cAMP-induced allosteric modulation of the pacemaker channels. Noticeably, this mechanism functionally resembles the pharmacological actions of muscarinic stimulation or β-blockers, but it does not require generalized changes in cytoplasmic cAMP levels thus ensuring a selective action on rate. In agreement with a competitive inhibition mechanism, TMYX exerts its maximal antagonistic action at submaximal cAMP concentrations and then progressively becomes less effective thus ensuring a full contribution of If to pacemaker rate during high metabolic demand and sympathetic stimulation., Competing Interests: CP, MP, DM, SL, GB, HJ, YW, YW, YW, AB No competing interests declared, DD DiFrancesco Dario was supported with a grant the research study on the Chinese medicine drug TMYX by Tianjin Zhongxin Pharmaceutical Group Co., Ltd. Le Ren Tang Pharmaceutical Factory who had absolutely no part in the study plan, data collection and analysis, and manuscript writing. The money was given to the University of Milan and no Honorarium was ever paid to DiFrancesco Dario. The author has no other competing interests to declare, AB was supported with a grant the research study on the Chinese medicine drug TMYX by Tianjin Zhongxin Pharmaceutical Group Co., Ltd. Le Ren Tang Pharmaceutical Factory who had absolutely no part in the study plan, data collection and analysis, and manuscript writing. The money was given to the University of Milan and no Honorarium was ever paid to Annalisa Bucchi. The author has no other competing interests to declare, MB was supported with a grant the research study on the Chinese medicine drug TMYX by Tianjin Zhongxin Pharmaceutical Group Co., Ltd. Le Ren Tang Pharmaceutical Factory who had absolutely no part in the study plan, data collection and analysis, and manuscript writing. The money was given to the University of Milan and no Honorarium was ever paid to Mirko Baruscotti. The author has no other competing interests to declare, (© 2022, Piantoni et al.)- Published
- 2022
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17. The funny current: Even funnier than 40 years ago. Uncanonical expression and roles of HCN/f channels all over the body.
- Author
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Benzoni P, Bertoli G, Giannetti F, Piantoni C, Milanesi R, Pecchiari M, Barbuti A, Baruscotti M, and Bucchi A
- Subjects
- Heart, Heart Rate, Humans, Neurons, Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels, Potassium Channels
- Abstract
Discovered some 40 years ago, the I
f current has since been known as the "pacemaker" current due to its role in the initiation and modulation of the heartbeat and of neuronal excitability. But this is not all, the funny current keeps entertaining the researchers; indeed, several data discovering novel and uncanonical roles of f/HCN channel are quickly accumulating. In the present review, we provide an overview of the expression and cellular functions of HCN/f channels in a variety of systems/organs, and particularly in sour taste transduction, hormones secretion, activation of astrocytes and microglia, inhibition of osteoclastogenesis, renal ammonium excretion, and peristalsis in the gastrointestinal and urine systems. We also analyzed the role of HCN channels in sustaining cellular respiration in mitochondria and their participation to mitophagy under specific conditions. The relevance of HCN currents in undifferentiated cells, and specifically in the control of stem cell cycle and in bioelectrical signals driving left/right asymmetry during zygote development, is also considered. Finally, we present novel data concerning the expression of HCN mRNA in human leukocytes. We can thus conclude that the emerging evidence presented in this review clearly points to an increasing interest and importance of the "funny" current that goes beyond its role in cardiac sinoatrial and neuronal excitability regulation., Competing Interests: Declaration of competing interest None., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2021
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18. Speeding Up the Heart? Traditional and New Perspectives on HCN4 Function.
- Author
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Hennis K, Rötzer RD, Piantoni C, Biel M, Wahl-Schott C, and Fenske S
- Abstract
The sinoatrial node (SAN) is the primary pacemaker of the heart and is responsible for generating the intrinsic heartbeat. Within the SAN, spontaneously active pacemaker cells initiate the electrical activity that causes the contraction of all cardiomyocytes. The firing rate of pacemaker cells depends on the slow diastolic depolarization (SDD) and determines the intrinsic heart rate (HR). To adapt cardiac output to varying physical demands, HR is regulated by the autonomic nervous system (ANS). The sympathetic and parasympathetic branches of the ANS innervate the SAN and regulate the firing rate of pacemaker cells by accelerating or decelerating SDD-a process well-known as the chronotropic effect. Although this process is of fundamental physiological relevance, it is still incompletely understood how it is mediated at the subcellular level. Over the past 20 years, most of the work to resolve the underlying cellular mechanisms has made use of genetically engineered mouse models. In this review, we focus on the findings from these mouse studies regarding the cellular mechanisms involved in the generation and regulation of the heartbeat, with particular focus on the highly debated role of the hyperpolarization-activated cyclic nucleotide-gated cation channel HCN4 in mediating the chronotropic effect. By focusing on experimental data obtained in mice and humans, but not in other species, we outline how findings obtained in mice relate to human physiology and pathophysiology and provide specific information on how dysfunction or loss of HCN4 channels leads to human SAN disease., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Hennis, Rötzer, Piantoni, Biel, Wahl-Schott and Fenske.)
- Published
- 2021
- Full Text
- View/download PDF
19. Molecular characterization of a complex small supernumerary marker chromosome derived from chromosome 18p: an addition to the literature.
- Author
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Marchina E, Forti M, Tonelli M, Maccarini S, Malvestiti F, Piantoni C, Filippini E, Fazzi E, and Borsani G
- Abstract
Background: Small supernumerary marker chromosomes (sSMC) are a heterogeneous group of structurally abnormal chromosomes, with an incidence of 0,044% in newborns that increases up to almost 7 times in developmentally retarded patients. sSMC from all 24 chromosome have been described, most of them originate from the group of the acrocentric, with around half deriving from the chromosome 15. Non-acrocentric sSMC are less common and, in the 30 percent of the cases, are associated with phenotypic effect. Complex sSMC consist of chromosomal material derived from more than one chromosome. Genotype-phenotype correlations in patients with sSMC are difficult to assess. Clinical features depend on factors such as its size, genetic content, the involvement of imprinted genes which may be influenced by uniparental disomy and the level of mosaicism. Trisomy of the short arm of chromosome 18 (18p) is an infrequent finding and does not appear to be associated with a specific syndrome. However, mild intellectual disability with or without other anomalies is reported in almost one-third of the patients., Case Presentation: Here we present clinical and molecular characterization of a new case of de novo complex sSMC consisting of the entire short arm of chromosome 18p associated with a centromere of either chromosome 13 or 21, evidenced in a 5-year-old boy during diagnostic workup for moderate intellectual disability and dysmorphisms. To date, only seven cases of isolated trisomy 18p due to a sSMC have been reported, three of which have been characterized by array CGH. In two of them the breakpoints and the size of the duplication have been described. In the manuscript we also reviewed cases reported in the DECIPHER database carrying similar duplication and also considered smaller duplications within the region of interest, in order to evaluate the presence of critical regions implicated in the pathological phenotype., Conclusions: Our case provides additional information about phenotypic effects of pure trisomy 18p, confirms chromosomal microarray analysis as gold standard to characterize complex sSMC, and supplies additional elements for genetic counselling.
- Published
- 2021
- Full Text
- View/download PDF
20. Independent influence of thermoregulatory cost on the lower and upper set-points of a heliothermic lizard.
- Author
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Leirião L, Piantoni C, Ribeiro PL, and Navas CA
- Subjects
- Animals, Energy Metabolism physiology, Temperature, Walking physiology, Body Temperature physiology, Body Temperature Regulation physiology, Lizards physiology
- Abstract
Studies on ectothermic vertebrates generally lead to average indicators of thermal preferences measured in the laboratory, which do not say about responses to natural environmental change and may not inform about individual variation and its triggering mechanisms. We studied whether and how changes in costs of thermoregulation influence the preferred temperature (T
p ) of individual lizards and their energetic investment in thermoregulation by exposing specimens to three treatments of increasing costs, recording body temperature (Tb ) and distance walked (energetic investment). Moderate costs induced an investment trade-off between energy-investment in thermoregulation and Tb , and highlighted individual variation that decreased with higher costs. Lower average Tp 's were observed in the high costs trials as a result of the decreased values of the lower and upper voluntary Tb 's. As costs increased, lizards walked a shorter overall distance and accepted lower Tb 's, but lizards still engaged in costly thermoregulation. Individual variation resulted in two main thermoregulatory patterns that in certain ecological contexts may have an important role in decision-making and adjusting to temperatures that are far from optimal temperatures for performance and physiological processes., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
21. Links between thermoregulation and aging in endotherms and ectotherms.
- Author
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Flouris AD and Piantoni C
- Abstract
While the link between thermoregulation and aging is generally accepted, much further research, reflection, and debate is required to elucidate the physiological and molecular pathways that generate the observed thermal-induced changes in lifespan. Our aim in this review is to present, discuss, and scrutinize the thermoregulatory mechanisms that are implicated in the aging process in endotherms and ectotherms. Our analysis demonstrates that low body temperature benefits lifespan in both endothermic and ectothermic organisms. Research in endotherms has delved deeper into the physiological and molecular mechanisms linking body temperature and longevity. While research in ectotherms has been steadily increasing during the past decades, further mechanistic work is required in order to fully elucidate the underlying phenomena. What is abundantly clear is that both endotherms and ectotherms have a specific temperature zone at which they function optimally. This zone is defended through both physiological and behavioral means and plays a major role on organismal senescence. That low body temperature may be beneficial for lifespan is contrary to conventional medical theory where reduced body temperature is usually considered as a sign of underlying pathology. Regardless, this phenomenon has been targeted by scientists with the expectation that advancements may compress morbidity, as well as lower disease and mortality risk. The available evidence suggests that lowered body temperature may prolong life span, yet finding the key to temperature regulation remains the problem. While we are still far from a complete understanding of the mechanisms linking body temperature and longevity, we are getting closer.
- Published
- 2014
- Full Text
- View/download PDF
22. Diversity of sponges (Porifera) from cryptic habitats on the Belize barrier reef near Carrie Bow Cay.
- Author
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Rützler K, Piantoni C, Van Soest RW, and Díaz MC
- Subjects
- Animals, Belize, Caribbean Region, Checklist, Biodiversity, Coral Reefs, Porifera anatomy & histology
- Abstract
The Caribbean barrier reef near Carrie Bow Cay, Belize, has been a focus of Smithsonian Institution (Washington) reef and mangrove investigations since the early 1970s. Systematics and biology of sponges (Porifera) were addressed by several researchers but none of the studies dealt with cryptic habitats, such as the shaded undersides of coral rubble, reef crevices, and caves, although a high species diversity was recognized and samples were taken for future reference and study. This paper is the result of processing samples taken between 1972 and 2012. In all, 122 species were identified, 14 of them new (including one new genus). The new species are Tetralophophora (new genus) mesoamericana, Geodia cribrata, Placospongia caribica, Prosuberites carriebowensis, Timea diplasterina, Timea oxyasterina, Rhaphidhistia belizensis, Wigginsia curlewensis, Phorbas aurantiacus, Myrmekioderma laminatum, Niphates arenata, Siphonodictyon occultum, Xestospongia purpurea, and Aplysina sciophila. We determined that about 75 of the 122 cryptic sponge species studied (61%) are exclusive members of the sciophilic community, 47 (39 %) occur in both, light-exposed and shaded or dark habitats. Since we estimate the previously known sponge population of Carrie Bow reefs and mangroves at about 200 species, the cryptic fauna makes up 38 % of total diversity.
- Published
- 2014
- Full Text
- View/download PDF
23. [Aureomycin in ectodermosis pluriorificialis].
- Author
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VALDES JM, PIANTONI C, and BELTRAMO N
- Subjects
- Chlortetracycline, Erythema Multiforme
- Published
- 1950
24. [Acute bacterial bronchopneumopathies (non tuberculous) in childhood].
- Author
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Guidi JA, Valdes JM, Piantoni C, Halac J, and Ramacciotti F
- Subjects
- Argentina, Child, Humans, Bronchitis, Bronchopneumonia, Pneumonia
- Published
- 1966
25. Thoracic deformities as a sequel to purulent pleurisy treated with penicillin.
- Author
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PIANTONI C, TORRES F, and DI MAJO S
- Subjects
- Pleurisy complications, Respiration Disorders, Thorax abnormalities
- Published
- 1945
26. [Bronchiectasis in children; clinico-radiologic aspects].
- Author
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CHATTAS A, DI RIENZO S, and PIANTONI C
- Subjects
- Child, Humans, Bronchiectasis, Radiology
- Published
- 1951
27. [Chloramphenicol as an immunosuppressive agent in the treatment of children with nephrotic syndrome].
- Author
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Piantoni C, Atea J, and Suti R
- Subjects
- Child, Child, Preschool, Humans, Chloramphenicol therapeutic use, Immunosuppressive Agents therapeutic use, Nephrotic Syndrome drug therapy
- Published
- 1970
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