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Teamgym is a new and emerging closed-skill sport, which includes tumbling, trampette, and floor programs performed by teams ranging from 6 to 12 athletes. The purpose of this study was to verify the concurrent validity of the session-rating of perceived exertion ([RPE] session-RPE) to quantifying the internal training load (ITL) of tumbling, trampette, and floor training sessions using Edwards' summated heart rate (HR)-zone method as a criterion measure. Session-RPE was obtained with 2 different instruments (i.e., CR-10 Borg's scale and visual analog scale) multiplied by the training duration (minutes). Ten female teamgym athletes (age = 21.7 ± 1.2 years; height = 164.9 ± 6.6 cm; and body mass = 54.6 ± 5.4 kg) participated in this study. High and significant correlations (r range: 0.77-0.85; R range: 0.59-0.85; p < 0.01) were found between Edwards' HR and the session-RPE methods and between the 2 session-RPE instruments (r range: 0.92-0.97; R2 range: 0.85-0.94; p < 0.01). The significant (p < 0.05) differences emerged between training sessions indicate that session-RPE discriminates ITL in relation to various technical skills. Hence, session-RPE can be a useful and inexpensive tool to quantify ITL in teamgym, and coaches could use this instrument to monitor their periodization plan as experienced by the athletes. [ABSTRACT FROM AUTHOR]

Purpose: Warming up before competition is universally recognized as an effective way to enhance performance. However, only a few articles have directly investigated different warm-up strategies adopted by triathletes and suggested by coaches. The Olympic-distance triathlon is an endurance competition characterized, at least for the elite, by a fast start with a strong correlation to the final position in the race. Thus, executing a proper warm-up protocol would be beneficial in optimizing performance. The present study aimed to provide an overview of the warm-up protocol adopted/suggested by national-caliber triathletes/coaches before an Olympic-distance triathlon race., Methods: Online surveys were created and shared between national- and international-caliber Italian, French, and Spanish triathletes and coaches. Information about the rationale, structure, and specific exercises adopted/suggested during personal warm-up protocols was collected. Thereafter, triathletes were grouped according to the discipline sequence reported., Results: Seventy-nine triathletes and nineteen coaches completed the survey. The cycle-run-swim was the most reported discipline sequence adopted, with a total time of 90.0 (25.0) minutes, against the 62.5 (25.0) minutes suggested by coaches. Conditioning exercises were performed by only 31.6% of triathletes 20 to 10 minutes before the race start., Conclusions: Triathletes who took part in this survey adopted very long protocols with the specific intention of including all disciplines. These results highlight the need to raise awareness in triathletes and coaches on the correct warm-up procedures and to stimulate researchers to design studies that directly investigate the effects of different warm-up protocols before competitions.

Purpose: In the present study, we examined how a 4-week intervention of maximal intended velocity (MIVRT) and controlled velocity resistance training (CRT)-induced task-specific responses in expert individuals., Methods: Twenty elite athletes were randomly assigned to either a MIVRT (n = 10) or CRT (n = 10) group, both following the same volume-load training based on the back-squat three times a week but with different intentions in moving load (force-exertion speed). We assessed one-repetition maximum (1RM), mean propulsive velocity (MPV), and mean propulsive power (MPP) using a progressive-loading test before and after the intervention. A linear position transducer was used to monitor propulsive velocity in training and testing sessions., Results: Both groups significantly increased their 1RM (CRT: + 12.3%, p < 0.001, d = 0.39; MIVRT: + 12.5%, p < 0.001, d = 0.45). Only the MIVRT group showed a significant improvement in MPV (p < 0.01) across different stepping loads, while both groups improved in MPP (MIVRT: + 22.4%, p < 0.001, d = 0.54; CRT: + 8.1%, p = 0.04, d = 0.17)., Conclusions: MIVRT induced significant adaptations in MPV and MPP at various loads (%1RM), underlining its specificity in targeting these parameters. Despite similar enhancements in 1RM, the distinct training protocols suggest that strength gains may stem from either maximal intent in moving loads or longer times under tension. This study highlights the role of execution speed in optimizing power outcomes, emphasizing task specificity as paramount to elicit physiological adaptations in chronically strength-trained individuals., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Background: In Paralympic sports, classification ensures fair competition by grouping athletes based on their impairments. The International Paralympic Committee has provided scientific principles to guide evidence-based classification procedures. In Paralympic Powerlifting, athletes compete in one class, divided by sex and bodyweight categories, overlooking impairment impact on performance., Objective: This study aimed to establish a consensus among international Paralympic powerlifting experts regarding classification and performance issues to guide future research., Methods: A two-round Delphi study was conducted involving 26 experts. The study sought to identify the adequacy of the current classification and competition systems, explore the impact of various impairments, and lay the initial groundwork for a performance determinants model., Results: Experts agreed that existing classification and competition systems in Paralympic powerlifting do not align with Paralympic standards. Impairments from neurological conditions and those causing anthropometric changes were suggested to have opposing performance impacts. Initial directions for a performance determinants model were outlined, focusing on arm and bar kinematics, anthropometry, and body composition., Conclusions: This study underscores the need for comprehensive research in Paralympic powerlifting, revealing critical discrepancies between current classification system and Paralympic standards. Insights into the multifaceted relationship between impairments and performance are provided to shape the future of Paralympic powerlifting research., Competing Interests: Financial disclosure statements have been obtained, and no conflicts of interest have been reported by the authors or by any individuals in control of the content of this article., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)

Abstract: Romagnoli, R and Piacentini, MF. Does fatigue affect the perception of velocity accuracy during resistance training? J Strength Cond Res 38(7): 1243-1247, 2024-The purpose of this study was to investigate whether perception of barbell velocity (PV) is affected by fatigue induced by 2 different training protocols. Twenty-two subjects were randomly divided into 2 groups: 10% velocity loss group (VL10) and repetitions to failure group (EX). Both protocols included 5 sets at 75% 1 repetition maximum but differed in the number of repetitions performed (Reps). Perception of barbell velocity was assessed in the back squat exercise during a test with 3 blinded loads (heavy, medium, light) 1 day rested (REST) and 1 day immediately following 1 of the 2 designated training protocols (POST). The accuracy of the PV was analyzed by calculating the delta score (ds), that is, the difference between perceived velocity (Vp) and real velocity of the barbell (Vr). During training, each group performed significantly different Reps per set (VL10: 3.9 ± 1.4; EX: 13.8 ± 6.3, p < 0.001) and consequently reported different levels of perceived exertion and repetitions in reserve ( p < 0.001). Real velocity and ds did not change between REST and POST-VL10 conditions at all loads. Although a significant decrease in Vr was found at light and medium loads ( p < 0.05) between REST and POST in the EX-Group, no significant differences were detected in the ds. These results demonstrate that Vp is a stable parameter on which practitioners can base their training despite different levels of fatigue., (Copyright © 2024 National Strength and Conditioning Association.)

Perception of Velocity (PV) is the ability to estimate single repetition velocity during resistance training (RT) exercises. The main purpose of the study was to evaluate the effects of Mental Fatigue (MF) on the accuracy of barbell PV. The secondary aims were to evaluate whether MF affected RT performance and ratings of perceived exertion (RPE; OMNI-RES) in the back squat. Twenty-four (14 Females, 10 Males) resistance-trained participants underwent 2 familiarization sessions and 1RM test for the back squat. In two separate sessions, PV was tested for light, medium, and heavy loads in 2 conditions in random order: at rest (REST) and in MF condition (POST-MF) induced by previous incongruent Stroop color-word task. MF and Motivation were assessed through visual analog scales (VAS; 0-100) before and after the Stroop task. For each load subjects performed 2 repetitions and reported the RPE value. Mean propulsive velocity (Vr) of the barbell was recorded with a linear encoder, while the perceived velocity (Vp) of the subjects was self-reported using the Squat-PV scale. The PV accuracy was calculated through the delta score (ds: Vp-Vr). Following the Stroop task MF increased significantly (p < 0.001; F (1, 23) = 52.572), while motivation decreased (p < 0.05; F (1, 23) = 7.401). Ds, Vr, and RPE did not show significant differences between conditions (p > 0.05) for the three loads analyzed. MF induced by previous demanding cognitive task did not affect PV accuracy. Furthermore, subjects maintained unchanged both RT performance and RPE values associated with each load, even when mentally fatigued., (© 2024 The Authors. European Journal of Sport Science published by Wiley‐VCH GmbH on behalf of European College of Sport Science.)

Digital devices have gained popularity in the last 10 years as a tool for exercise prescription, the monitoring of daily physical activity, and nutrition for the management of a health-related parameter. Therefore, the aim of this study was to assess the effectiveness of the use of digital devices to monitor exercise data in sedentary persons with HIV who exercise following an individualized activity pacing (AP) protocol on cardiorespiratory fitness body composition, blood lipid profile, and psychological parameters. Twenty-four PLWH were enrolled in an 18-week randomized, open-label, pilot AP exercise protocol. All participants were monitored by a Health Band connected to a mobile app that transmitted the data to a server. At week 3, they were randomized either in an experimental group (EG), in which an open device configuration enabled them to receive training data feedback ( n = 12), or continued with no data feedback (control group, n = 12). The primary endpoint was improvement from the baseline of 15% of steady-state oxygen consumption (V˙O 2 ) during a 6-min walking test. Technical issues occurred when pairing the health band with the app, which prevented EG participants from regularly receiving data feedback, and with data transmission to the server, which enabled only 40% monitoring of the total training days. Consequently, the study outcomes could not be compared between the two groups, and participants also lost confidence in the study. However, 19 out of 24 participants completed the AP program. Overall, only 6 (32%) improved steady-state V˙O 2 , with no significant changes at W18 from the baseline. Significant reductions were observed of BMI ( p = 0.040), hip circumference ( p = 0.027), and total-( p = 0.049) and HDL-cholesterol ( p = 0.045). The failure of digital device performance substantially affected study procedures, monitoring, and participants' engagement, and likely limited the potential benefits of the AP exercise program.

We assessed the training, work and Life Stress demands of a mixed gender group of 48 top amateur short-distance triathletes using an online retrospective epidemiological survey and the Life Events Survey for Collegiate Athletes. On superficial inspection, these mainly masters athletes appeared to undergo all the types of training that are recommended for the aging athlete. However, there were significant scheduling differences between their weekday vs. their weekend training, suggesting that age-groupers' outside sports commitments may affect their training efficacy. The triathletes claimed to periodize, to obtain feedback on and to modify their training plans when appropriate-and some evidence of this was obtained. Over the year preceding the ITU World Age-Group Championships, they averaged 53%, 33% and 14% of their combined swim, cycle and run training time, respectively, within intensity zones 1, 2 and 3. Although the triathletes specifically stated that their training was focused on preparation for the ITU World Age-Group Championships, the way that they modified their training in the month before the event suggested that this aim was not necessarily achieved. Sports-related stress accounted for most-42.0 ± 26.7%-of their total Life Stress over the preceding year (vs. 12.7 ± 18.6% for Relationship-, 31.3 ± 25.9% for Personal- and 14.0 ± 21.1% for Career-related Stress). It affected most athletes, and was overwhelmingly negative, when it related to failure to attain athletic goal(s), to injury and/or to illness.

Introduction: In Paralympic powerlifting competitions, movement execution symmetry is a technical requirement influenced by individual athlete characteristics and motor strategies. Identifying the elements associated with individual motor strategies can offer valuable insight for improving sport performance. Therefore, this case series study aimed to explore muscle activation symmetry and its intra- and inter-individual variability to determine the muscles mostly related to individual motor strategies in elite Paralympic powerlifters., Methods: Bilateral electromyographic activation of the anterior deltoid (AD), pectoralis major (PM), latissimus dorsi (LD), triceps (TRI) and external oblique (EO) muscles were analysed in five elite Paralympic powerlifters while performing four sets of one-repetition maximum of Paralympic bench press. Muscle activation symmetry indexes (SI) were obtained and transformed to consider individual-independent evaluation. The coefficient of variation (CV), variance ratio (VR), and mean deviation (MD) were computed to assess inter- and intra-individual variability in electromyographic waveforms and SI., Results: Both transformed and non-transformed SI indicated overall symmetric activation in DA, PM, TRI, and LD. Transformed SI revealed asymmetrical muscle activation of EO when grouping data (mean bilateral difference: 10%). Athletes exhibited low intra-individual SI variability in all analysed muscles (CV < 10%) and low inter-individual variability in DA, PM, LD, and TRI (CV < 10%; VR: 4%-11%; MD: 29%-43%). In contrast, higher inter-individual variability was observed in EO (CV: 23%; VR: 23%; MD: 72%-81%)., Conclusion: The highest variability and asymmetry in abdominal muscle activation among athletes emphasize the importance of personalized training approaches for targeting these muscles due to their role in individualizing motor strategies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Rum, Romagnoli, Lazich, Sciarra, Balletti, Piacentini, Boraschi and Bergamini.)

Purpose: Few data are available on sleep characteristics of elite track-and-field athletes. Our study aimed to assess (1) differences in sleep between sexes and among different track-and-field disciplines, (2) the effect of individualized sleep-hygiene strategies on athletes' sleep parameters, and (3) daytime nap characteristics in track-and-field athletes., Methods: Sleep characteristics of 16 elite Olympic-level track-and-field athletes (male: n = 8; female: n = 8) were assessed during the preseason period, at baseline (T0), and during the in-season period, after the adoption of individualized sleep-hygiene strategies (T1). Sleep parameters were objectively monitored by actigraphy for a minimum of 10 days, for each athlete, at both T0 and T1. A total of 702 nights were analyzed (T0 = 425; T1 = 277)., Results: Female athletes displayed better sleep efficiency (88.69 [87.69-89.68] vs 91.72 [90.99-92.45]; P = .003, effect size [ES]: 0.44), lower sleep latency (18.99 [15.97-22.00] vs 6.99 [5.65-8.32]; P < .001, ES: 0.65), higher total sleep time (07:03 [06:56-07:11] vs 07:18 [07:10-07:26]; P = .030, ES: 0.26), earlier bedtime (00:24 [00:16-00:32] vs 00:13 [00:04-00:22]; P = .027, ES: 0.18), and lower nap frequency (P < .001) than male athletes. Long-distance runners had earlier bedtime (00:10 [00:03-00:38] vs 00:36 [00:26-00:46]; P < .001, ES: 0.41) and wake-up time (07:41 [07:36-07:46] vs 08:18 [08:07-08:30]; P < .001, ES: 0.61), higher nap frequency, but lower sleep efficiency (88.79 [87.80-89.77] vs 91.67 [90.95-92.38]; P = .013, ES: 0.44), and longer sleep latency (18.89 [15.94-21.84] vs 6.69 [5.33-8.06]; P < .001, ES: 0.67) than athletes of short-term disciplines. Furthermore, sleep-hygiene strategies had a positive impact on athletes' total sleep time (429.2 [423.5-434.8] vs 451.4 [444.2-458.6]; P < .001, ES: 0.37) and sleep latency (14.33 [12.34-16.32] vs 10.67 [8.66-12.68]; P = .017, ES: 0.19)., Conclusions: Sleep quality and quantity were suboptimal at baseline in Olympic-level track-and-field athletes. Large differences were observed in sleep characteristics between sexes and among different track-and-field disciplines. Given the positive effect of individualized sleep-hygiene strategies on athlete's sleep, coaches should implement sleep education sessions in the daily routine of top-level athletes.

Football is a popular sport, but little is known about the youth-to-senior transition rates in elite players, particularly in large and successful countries. This study aims to investigate the youth-to-senior transition rate in the Italian national football team, both prospectively and retrospectively, and to explore if Relative Age Effects (RAEs) affect this transition. Data from 885 players selected in youth and senior Italian national teams between 2000 and 2021 were included in the study. For each player, the birthdate and the number of selection in Under 16, 17, 19, 21 and senior team was considered. The transition rate was determined by the number of youth players competing in the Senior National team (and vice versa), whilst birth quarter (Q) distributions with a chi-square goodness-of-fit test. Prospectively, the transition rate increased as age increased (i.e., from ~20% in U16 to ~50% in U19). Retrospectively, less than 10-20% of youth players were subsequently selected for the senior team. Data revealed a skewed birth date distribution in all age groups, and the RAEs magnitude decreased when age increased (i.e., ORs for Q1 vs Q4 was ~ 9 in U16 and ~ 1.7 in senior teams). Nevertheless, the RAE magnitude was smaller for successfully transitioned players. In conclusion, most players in the senior team were not previously selected for youth teams suggesting that junior international experience may not be a prerequisite for later success. Moreover, while the birthdate strongly influences the selection of youth national teams, its impact is less evident in the youth-to-senior transition., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Boccia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

To date, the literature has failed to individuate a clear motivation for the performance decrement after a mental fatigue-inducing task. This study aimed to evaluate biomechanical and perceptual variables during a swimming training session in different mental fatigue states. Seven amateur triathletes watched a documentary, utilized a smartphone, or performed an AX-CPT for 45 min randomly on three different days. After, they performed a 15-min warm-up followed by 6 × 200 m at constant pre-set speed plus one 200 m at maximal effort. The mental fatigue status was assessed by the visual analog scale (VAS) and short-Stroop task results before, post-mental task, and post-swimming session. The biomechanical and motor coordination variables during swimming were assessed using five IMU sensors and video analysis. The heart rate and rate of perceived exertion were monitored during the task. No differences in biomechanical and perceptual variables were found between and within conditions. Higher mental fatigue was found only in the AX-CPT condition at post task by VAS. In this preliminary study, no changes in swimming biomechanics were highlighted by mental fatigue, but the warm-up performed may have counteracted its negative effects. Further studies are recommended.

Purpose: Wetsuits have been shown to change swim biomechanics and, thus, increase performance, but not all athletes are comfortable with their use because of possible modifications in motor coordination. The aim of this study was to evaluate the effects of wetsuit use on biomechanical, physiological, and perceptual variables., Methods: Eleven national- and international-level triathletes, familiar with wetsuit use, performed 7 × 200-m front crawl at constant preset speed twice, with and without a full wetsuit. The trunk incline (TI) and index of coordination (IdC) were measured stroke by stroke using video analysis. Stroke, breaths, and kick count, and timing (as breathing/kick action per arm-stroke cycle); stroke length (SL); and underwater length were analyzed using inertial-measurement-unit sensors. Heart rate (HR), rating of perceived exertion (RPE), and swimming comfort were monitored during the task., Results: A lower TI; IdC; number of strokes, kicks, and breaths; HR; and RPE for each 200 m were found in wetsuit compared with swimsuit condition. Higher values of SL and underwater length were found in wetsuit, whereas no differences were found in swimming comfort and timing of kicks and breaths. An increase for swimsuit condition in number of strokes and breaths, HR, and RPE was found during the task compared with the first 200 m., Conclusion: Wetsuit use reduces TI and, thus, drag; increases propelling proficiency; and shows lower fatigability, without modifying motor coordination, compared with swimsuit use at the same speed. The use of a wetsuit during training sessions is recommended to increase comfort and the positive effects on performance.

Background: the aim of the study was to develop and validate a specific perception velocity scale for the Back Squat exercise to discriminate the velocity of each repetition during a set., Methods: 31 resistance trained participants completed 3 evaluation sessions, consisting of 3 blinded loads (light, medium, heavy). For each repetition, barbell mean velocity (Vr) was measured with a linear position transducer while perceived velocity (Vp) was reported using the Squat Perception of Velocity (PV) Scale., Results: Pearson correlation coefficients (r) showed very high values for each intensity in the 3 different days (range r = 0.73-0.83) and practically perfect correlation for all loads (range r = 0.97-0.98). The simple linear regression analysis between Vp and Vr revealed values ranging from R 2 = 0.53 to R 2 = 0.69 in the 3 intensities and values ranging from R 2 = 0.95 to R 2 = 0.97 considering all loads. The reliability (ICC 2 .1 , SEM) of Vp was tested for light (0.85, 0.03), medium (0.90, 0.03) and heavy loads (0.86, 0.03) and for all loads (0.99, 0.11). The delta score (ds = Vp - Vr) showed higher accuracy of the PV at heavy loads., Conclusions: these results show that the PV Squat Scale is a valid and reliable tool that can be used to accurately quantify exercise intensity.

The triathlon Mixed-Team-Relay (MTR) is a new race format present for the first time at the Tokyo Olympic Games in 2021. The results of the ITU Triathlon Mixed Relay World Championship from 2014 to 2019 were collected to provide practical suggestions for forming a successful MTR, such as the importance of each leg and discipline on MTR and Super-Sprint performance. The total relay time (Trelay), the time of each team member (leg-from 1 to 4) (Tleg), and the time of each single discipline (swim, T1, cycle, T2, run) were collected from the official website. Inferential analysis was performed to assess prediction and differences between variables. Leg 3 was shown to be the most important to predict Trelay (0.41), which is also the slower. For both Trelay and Tleg, cycling resulted as the most important (>0.60) and longer (~52%) portion, followed by running and swimming. However, higher importance in swimming was found in successful teams compared to running. For a successful MTR, we suggest: (a) use short-distance specialized triathletes; (b) strengthen cycling and swimming; (c) position in legs 1 and 2 athletes capable of racing in a group; in legs 3 and 4 athletes capable of racing in a non-drafting situation.

Background: Kicking a ball is a very frequent action in sport and leisure time activities and a low proficiency in this skill could limit the participation in recreational sport activities. This issue is emphasised in individuals with Down syndrome (IDS) for which data about motor competence in kicking are limited to children. Here, we aim at evaluating the kicking competence of IDS combining a qualitative and a quantitative method., Methods: Twenty-three adult IDS and 21 typically developed individuals (ITD) volunteered to participate in the study. Peak-to-peak 3D linear acceleration and angular velocity were recorded at 200 samples/s using two inertial measurement units placed on the lower back and lateral malleolus of the dominant limb during kicking. Motor competence in kicking was assessed according to the criteria proposed in the test of gross motor development version 3 (TGMD-3)., Results: Individuals with Down syndrome showed lower motor competence (ITD: 5.9 ± 1.2; IDS: 3.2 ± 2.0) and lower angular velocities about the cranio-caudal (ITD: 3.0 ± 1.8; IDS: 2.1 ± 1.1 rad/s) and medio-lateral axes (ITD: 4.5 ± 1.5; IDS: 3.0 ± 1.1 rad/s) of the trunk compared with ITD. Shank angular velocity about the medio-lateral axis was lower in IDS (ITD: 14.3.6 ± 4.0; IDS: 9.9 ± 2.8 rad/s)., Conclusions: The lower trunk angular velocity in IDS may limit the possibility to rely on the proximal-to-distal sequencing commonly observed in kicking and generate high shank angular velocity upon ball impact. The lower trunk angular velocity may result from orthopaedic features of the pelvic girdle and possibly from a poorer neuromuscular control of core muscles., (© 2022 MENCAP and International Association of the Scientific Study of Intellectual and Developmental Disabilities and John Wiley & Sons Ltd.)

The perception of bar velocity (PV) is a subjective parameter useful in estimating velocity during resistance training. The aim of this study was to investigate if the PV can be improved through specific training sessions, if it differs between the back squat (SQ) and bench press (BP), and if there are differences in perception accuracy in the different intensity zones. Resistance-trained participants were randomly divided in an experimental (EG, n = 16) or a control group (CG, n = 14). After a familiarization trial, both groups were tested before and after 5 weeks of training. The PV was assessed with five blinded loads covering different intensity domains. During the training period, only the EG group received velocity feedback for each repetition. Prior to training, both groups showed a greater PV accuracy in the SQ than in the BP. Post training, the EG showed a significant reduction (p < 0.05) in the delta score (the difference between the real and perceived velocity) for both exercises, while no significant differences were observed in the CG. Prior to training, the perceived velocity was more accurate at higher loads for both exercises, while no difference between loads was observed after training (EG). The results of this study demonstrate that the PV improves with specific training and that differences in the accuracy between loads and exercise modes seen prior to training are leveled off after training.

Despite the common belief that sleep quality at altitude is poor, the scientific evidence to support this notion is still modest. Therefore, the purpose of the present study was to evaluate possible changes of actigraphy-based and subjective sleep parameters in a group of elite open-water swimmers during a 14-day altitude training camp (ATC) at 1500 m. The study subjects were five Olympic-level open-water swimmers (mean age: 25.0 ± 3.2 years; 3 females and 2 males). All subjects wore a wrist activity monitor and filled a sleep diary for 18 consecutive nights, 4 nights before and 14 nights during ATC. The data were then analyzed at four different time points: before ATC (PRE), the first two days of ATC (T1), and after one (T2) and two weeks of ATC (T3). Training load, assessed as the covered distance (km), session rating of perceived exertion (sRPE), and heart rate (HR), was monitored during the week before and the first and second week of ATC. No significant differences in objective and subjective scores of sleep quality were detected, whereas the sleep onset time (p = 0.018; η2p = 0.83, large) and sleep offset time (p < 0.001; η2p = 0.95, large) significantly differed among PRE, T1, T2, and T3: elite athletes started to sleep and woke up ≃ 1 h earlier the first two days of ATC compared to PRE (sleep onset time: p = 0.049; sleep offset time: p = 0.016). Further, an increase in the training volume during the two weeks of the ATC was observed, with the most time spent in a low-intensity regime and an increase in time spent in a high-intensity regime compared to PRE. Sleep quality was not negatively influenced by a 14-day altitude training camp at 1500 m in a group of Olympic-level elite swimmers despite an increase in perceived exertion during training sessions. Nonetheless, early sleep onset and sleep offset times were observed for the first two nights of ATC: elite athletes started to sleep and woke up ≃ 1 h earlier compared to the baseline nights.

The aim of the present study was to test the effect of carbohydrate ingestion, simulating a 10-km open water race competition on energy cost (Csw), perceived exertion (RPE), heart rate (HR), stroke rate (SR) and performance. We hypothesized that carbohydrate ingestion would reduce Csw and RPE in elite open water swimmers (OW-swimmers) and improve performance. Eight elite OW-swimmers swam for 3 × 30 min with 20-s of interval necessary to collect data in the swimming flume at a pre-set pace corresponding to their 10-km race pace, followed by a time to exhaustion test (TTE) at 100% of the peak oxygen uptake (V̇O 2peak ). During the set, OW-swimmers ingested 45-g of carbohydrates (CHO) in 550-mL of water (8% solution) during each of the two intervals or a placebo solution (PLA). HR, RPE, V̇O 2 and SR were measured. Shapiro-Wilk test was used to verify the normal distribution of data. Two-way repeated measures ANOVA and t -test was performed ( p  < 0.05). A significant difference emerged in TTE between the trials (169.00 ± 91.06 s in CHO; 102.31 ± 57.47 s in PLA). HR, RPE and SR increased during the TTE but did not differ between trials. Csw did not show a significant main effect between the two conditions and in time course in both conditions. CHO ingestion significantly increased TTE at 100% of V̇O 2peak after 90-min of swimming at 10-km race pace. These findings indicate that CHO intake during a 10-km open water swimming competition should have a beneficial impact on performance in the final part of the race.

In children, motor competence (MC) and the amount of physical activity are tightly interconnected. In adults with Down syndrome (DS), MC has been poorly addressed, resulting in a limited understanding of the possibility to improve MC over time. Here, we aim to: (1) investigate MC in adults with DS by comparing them with a group of typically developed peers and (2) verify the effect of an adapted karate program on MC. Adults with DS (DSG; n = 57) and typically developed adults (TDG; n = 21) performed the Test of Gross Motor Development version 3 (TGMD-3). The total TGMD-3 score ( TOT TGMD-3), the locomotor ( LOC TGMD-3), and object control ( OBJ TGMD-3) scores were computed. After a 40 week adapted karate program, DSG ( n = 37) underwent the post-training TGMD-3 assessment. Compared to TDG, DSG showed lower TOT TGMD-3 (DSG: 45.5 ± 17.3; TDG: 77.3 ± 9.5), LOC TGMD-3 (DSG: 22.2 ± 10.0; TDG: 36.2 ± 7.6) and OBJ TGMD-3 (DSG: 23.3 ± 10.9; TDG: 41.1 ± 5.6). After the training, TOT TGMD-3, LOC TGMD-3 and OBJ TGMD-3 increased by 35.6%, 30.0% and 40.7%, respectively. Our results suggest that MC acquisition does not evolve into a mature form in adulthood in individuals with DS. Moreover, a brief exposure to an adapted karate program induces an increase in motor competence in DS, even in adulthood.

Quantitative evaluation of synergic action among the different body segments is fundamental to swimming performance. The aim of the present study was to develop an easy-to-use tool for stroke-by-stroke evaluation of a swimmer's integrated timing of stroking, kicking, and breathing. Twelve swimmers were evaluated during one trial of 100 m front-crawl swimming at self-selected speed. Five three-axial inertial sensors were mounted on the head, wrists, and ankles. Algorithms for the wrist entry into the water, the lower limb beat during the downward action, and the exit/entry of the face from/into the water were developed. Temporal events identified by video-based technique, using one sagittal moving camera, were assumed as the gold standard. The performance was evaluated in terms of the root-mean-square error, 90th percentile of absolute error, coefficient of variation, Bland-Altman plots, and correlation analysis. Results of all temporal events showed high agreement with the gold standard, confirmed by a root-mean-square error of less than 0.05 s for absolute temporal parameters and less than 0.7% for the percentages of the stroke cycle duration, and with correlation coefficients higher than 0.856. The protocol proposed was not only accurate and reliable, but also user-friendly and as unobtrusive as possible for the swimmer, allowing a stroke-by-stroke analysis during the training session.

Purpose: To analyze training-intensity distribution (TID) using different independent monitoring systems for internal training load in a group of elite open-water swimmers., Methods: One hundred sixty training sessions were monitored in 4 elite open-water swimmers (2 females and 2 males: 23.75 [4.86] y, 62.25 [6.18] kg, 167 [6.68] cm) during 5 weeks of regular training. Heart-rate-based methods, such as time in zone (TIZ), session goal (SG), and hybrid (SG/TIZ), were used to analyze TID. Similarly to SG/TIZ, a new hybrid approach, the rating of perceived exertion (RPE)/TIZ for a more accurate analysis of TID was used. Moreover, based on the 3-zone model, the session ratings of perceived exertion of the swimmers and the coach were compared., Results: Heart-rate- and RPE-based TID methods were significantly different in quantifying Z1 (P = .012; effect size [ES] = 0.490) and Z2 (P = .006; ES = 0.778), while no difference was observed in the quantification of Z3 (P = .428; ES = 0.223). The heart-rate-based data for Z1, Z2, and Z3 were 83.2%, 7.4%, and 8.1% for TIZ; 80.8%, 8.3%, and 10.8% for SG/TIZ; and 55%, 15.6%, and 29.4% for SG. The RPE-based data were 70.9%, 19.9%, and 9.2% for RPE/TIZ% and 41.2%, 48.9%, and 9.7% for the session rating of perceived exertion. No differences were observed between the coach's and the swimmers' session ratings of perceived exertion in the 3 zones (Z1: P = .663, ES = -0.187; Z2: P = .110, ES = 0.578; Z3: P = .149, ES = 0.420)., Conclusion: Using RPE-based TID methods, Z2 was significantly larger compared with Z1. These results show that RPE-based TID methods in elite open-water swimmers are affected by both intensity and volume.

Purpose: Although wearing a wetsuit while swimming, when permitted, is primarily for safety reasons (ie, to protect against hypothermia), changes in buoyancy, biomechanics, and exercise performance have been reported. This narrative review covers the benefits of different wetsuit models on performance in swimming and triathlon., Methods: A computer search of online databases was conducted to locate relevant published research until March 2021. After the screening process, 17 studies were selected for analysis., Results: Most of the selected studies involved pool swimmers or triathletes completing short or middle distances in a pool while using a full or a long sleeveless wetsuit. Swimming with wetsuit elicited significant improvements in performance (maximum 11%), mainly by decreasing drag and energy cost, by increasing buoyancy, and by affecting technique. Different rates of change in each factor were found according to swimming ability and wetsuit model. In addition, wearing a wetsuit was often rated as uncomfortable by athletes., Conclusions: Although improvement in swimming performance by wearing a wetsuit has been reported in the literature, the amplitude of the improvement remains questionable. The enhancement in swimming performance is attributable merely to improvements in propulsion proficiency and buoyancy, as well as a reduction in drag. The extent to which athletes are familiar with the use of a wetsuit, their swimming ability, and the wetsuit model may play important roles in this improvement. More studies simulating competition and comparing elite versus nonelite athletes are needed.

Introduction: Individuals with Down syndrome (DS) show a delayed acquisition of gross motor skills. Among gross motor skills, hopping is a particular form of jumping that can be performed using one leg. Despite its large use during play and physical activity, this skill in adults with DS has not received much attention so far. Here, we aim at investigating hopping skill in adults with DS both from a quantitative and qualitative point of view., Methods: Center of mass and dominant leg kinematics during hopping over distance were recorded from 24 adult individuals with DS and from 21 typically developed adults (TD) using two inertial measurement units positioned on the posterior aspect of the lower back and on the lateral malleolus of the hopping leg. From linear acceleration and angular velocity signals, hopping frequency (HF), cycle, stance and flight duration (CD, SD, FD), vertical stiffness (K V ) and peak to peak linear acceleration and angular velocities about the cranio-caudal, antero-posterior and medio-lateral axes were extracted. A qualitative process assessment of the hopping skill was carried out using the performance criteria of the test for gross motor development (TGMD-3). The extracted parameters were submitted to analysis of covariance, with stature as a covariate to rule-out possible confounding effects., Results: The qualitative assessment highlighted a poorer hopping performance in the DS group compared to the TD group. DS participants showed higher HF and K V , shorter CD, SD, FD and lower angular velocity about the cranio-caudal axis compared to the TD group. Significant correlations between the temporal parameters of the quantitative assessment and the results of the qualitative assessment were observed., Discussion: The poorer motor competence in hopping in individuals with DS compared to TD peers may be related to the shorter flight time and higher vertical stiffness observed in TD peers. The adopted instrumental approach, overcoming the limitations of subjective evaluations, represents a promising opportunity to quantify motor competence in hopping., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Purpose: The sensation of fatigue experienced at a certain point of the race is an important factor in the regulation of pacing. The rating of perceived exertion (RPE) is considered one of the main mediators utilized by athletes to modify pacing. The aim was to analyze the relationship between pacing and RPE of elite open water swimmers during national indoor pool championships., Methods: A total of 17 elite open water swimmers (males, n = 9; females, n = 8) agreed to provide RPE every 500 m during the finals of the national championships 5-km indoor pool race. Time splits, stroke rate, and RPE were collected every 500 m. The Hazard score was calculated by multiplying the momentary RPE by the remaining fraction of the race. Athletes were placed in one of two categories: medalists or nonmedalists. For all variables, separate mixed analysis of variances (P ≤ .05) with repeated measures were used considering the splits (ie, every 500 m) as within-subjects factor and the groups (ie, medalists and nonmedalists) as between-subjects factor., Results: Average swimming speed showed a significant main effect for split for both males and females (P < .001). A significant interaction was observed between average swimming speed and groups for females (P = .032). RPE increased in both groups (P < .001) with no difference observed between groups. However, the female nonmedalists showed a disproportionate nonlinear increase in RPE (5.20 [2.31]) halfway through the event that corresponded to the point where they started significantly decreasing speed., Conclusions: The results of the present study show different pacing strategies adopted by medalists and nonmedalists despite a similar RPE.

TeamGym is a popular form of gymnastics, including tumbling (Tu), trampette (Tr) and floor exercises (F) characterized by intensive practice placing high levels of stress on athletes. The aim of the study was to investigate athletes' stress-related changes during TeamGym training and competition, considering hormonal and enzymatic responses (i.e., salivary cortisol and alpha-amylase). Ten (5 males and 5 females) TeamGym athletes (age: 22-28 y) were tested twice at the same time before training and competition; furthermore, for excluding circadian effect on hormonal and enzymatic responses, they were tested at the same time during a rest day. Alpha-amylase and cortisol were measured 15 min before the beginning of exercise, after each gymnastic equipment performance, and after thirty minutes from the end of the performance. Factorial ANOVA with repeated measures was used to verify differences between training and competition ( p < 0.05). Competition elicited higher values of alpha-amylase than training ( p ranging from 0.001 to 0.019) and rest ( p ranging from 0.001 to 0.019). Cortisol showed no exercise induced increase, and its concentrations were higher prior to training compared to competition. TeamGym responses confirm other sports findings in stating that competition elicits higher stress response than training and suggest that salivary alpha-amylase is a more sensitive marker than cortisol to psychophysiological stress also in gymnastics intermittent performance.

We are glad to introduce the Third Journal Club of Volume five, the third issue. This edition is focused on relevant studies published in the last years in the field of PhysioMechanics of Human Locomotion, chosen by our Editorial Board members and their colleagues. We hope to stimulate your curiosity in this field and to share with you the passion for the Sports Medicine and Movement Sciences seen also from the scientific point of view. The Editorial Board members wish you an inspiring lecture., Competing Interests: Conflicts of Interest: The authors declare no conflict of interest.

This study aims to assess the correspondence between session rating of perceived exertion (sRPE) breakpoints with both the first lactate threshold (LT1) and the second lactate threshold (LT2) in elite open water swimmers (OWS). Six elite OWS of the National Olympic Team specialized in distances between 5 and 25 km participated to the study. OWS performed a set of 6 times 500 m incremental swimming step test during which blood lactate concentration (BLC), split time (ST), stroke frequency (SF), and rating of perceived exertion (RPE) were collected. To assess the corresponding breakpoints, we considered LT1 as the highest workload not associated with rise in BLC and LT2 as the increase of 2mM above LT1. According to the LT1 and LT2, the identified zones were: Z1 ≤3, Z2 between 4 and 6, Z3 ≥ 7. In conclusion, the intensity zones determined for OWS resulted different from what previously reported for other endurance disciplines.

Purpose: This study aimed to assess 16-wk improvements of physical fitness, metabolic, and psychological parameters in people living with HIV (PLWH) exercising with the support of a smartphone application, as compared with a control group exercising without application., Methods: This was a randomized, open-label, pilot study enrolling PLWH in a 16-wk protocol consisting of moderate physical activity three times per week, which included an initial coach-supervised period of 4 wk, followed by 12 wk where participants trained independently. Participants were allocated to either an experimental group that trained using a smartphone application (APP) or a control group that practiced following a hard copy training program (No-APP). At baseline (BL) and after 16 wk (W16), patients were assessed for cardiorespiratory fitness, body composition, blood lipid profile, and POMS., Results: Forty-eight PLWH were screened and 38 were eligible: 20 were allocated to the APP group and 18 to the No-APP group. Two APP and two No-APP participants were lost to follow-up. Intention-to-treat analysis showed a W16 improvement from BL of ≥15% V˙ O2peak in 13 (72%) of 18 in APP, but only in 3 (19%) of 16 in No-APP participants (P = 0.025). Significant W16 improvements were observed in APP, but not in No-APP participants, in V˙O2peak; fat mass and fat-free mass percent; total cholesterol, LDL cholesterol, and triglycerides; vigor; and total mood by POMS. Accordingly, significant percent change differences between the APP and the No-APP groups were observed in V˙O2peak; fat and fat-free mass percent; total cholesterol, LDL cholesterol, and triglycerides; and depression, vigor, anger, and total mood by POMS., Conclusions: Exercising using a smartphone application improved cardiorespiratory fitness, body composition, cholesterol profiles, and psychological outcomes in PLWH.

The aim of this study is to investigate whether the change in (sub)maximal heart rate after intensified training is associated with the change in performance. Thirty subjects were recruited who performed cardiopulmonary exercise tests to exhaustion 2 weeks before (pre), 1 week after (post) and 5 weeks after (follow-up) an 8-day non-competitive amateur cycling event (TFL). The exercise volume during the TFL was 7.7 fold the volume during the preparation period. Heart rate and cardiopulmonary parameters were obtained at standardised absolute submaximal workloads (low, medium and high intensity) and at peak level each test. Subjects were classified as functionally overreached (FOR) or acute fatigued (AF) based on the change in performance. No differences between FOR and AF were observed for heart rate ( P  = .51). On total group level (AF + FOR), post-TFL heart rate decreased significantly at low (-4.4 beats·min -1 , 95% CI [-8.7, -0.1]) and medium (-5.5 beats·min -1 [-8.5, -2.4]), but not at high intensity. Peak heart rate decreased -3.4 beats·min -1 [-6.1, -0.7]. O 2 pulse was on average 0.49 ml O 2 ·beat -1 [0.09, 0.89] higher at all intensities after intensified training. No changes in ⩒O 2 ( P  = .44) or the ventilatory threshold ( P  = .21) were observed. Pearson's correlation coefficients revealed negative associations between heart rate and O 2 pulse at low ( r  = -.56, P  < .01) and medium intensity ( r  = -.54, P  < .01), but not with ⩒O 2 or any other submaximal parameter. (Sub)maximal heart rate decreased after the TFL. However, this decrease is unrelated to the change in performance. Therefore, heart rate seems inadequate to prescribe and monitor intensified training.

Purpose: Different aspects of pacing in endurance events have been investigated, however, there are very limited information on pacing strategies during open-water swimming. The aim was to describe and compare the pacing profile used by male and female open-water swimmers (OW-swimmers) during the 5-, 10- and 25 km races in the main international competitions., Methods: A total of 438 performances were analysed for 5 km, 579 for 10 km and 189 for 25 km, from 2012 to 2017. Swimmers were divided into four groups based on finishing time. G1 whose finishing times were within 0.5% of the winner's time, G2 between 0.51% and 1% slower than winner's time; G3 between 1.1% and 2% slower than winner's time; G4 over 2% of winner's time. Kolmogorov-Smirnov test was used to verify the normal distribution of data and repeated measures ANOVA was performed., Results: G1 adopted a negative pacing and significantly increased the speed in the last split compared with the other groups during the 5-, 10- and 25-km races in both males and females (p < .001). During the 5- and 10-km race, the last split speed of G1 was significantly faster compared to the other groups in both males and females (p < .05)., Conclusions: OW-swimmers that used a conservative approach remaining in G1 until the finish of the race, increase the possibility to win a medal in the main international competitions.

In 2009, the International Triathlon Union created a new triathlon race format: The World Triathlon Series (WTS), for which only athletes with a top 100 world ranking are eligible. Therefore, the purpose of this study was to analyze the influence of the three disciplines on performance within all the WTS Olympic distance races within two Olympic cycles, and to determine whether their relative contribution changed over the years. Methods: For each of a total of 44 races, final race time and position as well as split times (and positions), and summed time (and position) at each point of the race were collected and included in the analysis. Athletes were divided into 4 groups according to their final race placing (G1: 1st⁻3rd place; G2: 4⁻8th place; G3: 8⁻16th place and G4: ≥17th place). Two-way multivariate ANOVAs were conducted to compare the main effects of years and rank groups. For females, there were significant differences in the swim and bike segment only between G4 and the other groups ( p range from 0.001⁻0.029), whilst for the run segment each group differed significantly from each other ( p < 0.001). For males, there were significant differences in swim only between G4 and the other groups ( p range from 0.001⁻0.039), whilst for the running segment each group differed significantly from the others ( p < 0.001). Although we found running to be the segment where there were significant differences between performance groups, it is apparently important for overall success that a good runner be positioned with the first cycling pack. However, bike splits were not different between either of the four male groups or between the first 3 groups of the females. At this very high level of performance, at least in the males, the bike leg seems to be a smooth transition towards running.

Background: It has been shown that the fastest open-water swimmers (OW-swimmers) increase significantly the speed in the last split of the open-water events. The aim of the present work was to determine if the fastest OW-swimmers have a higher speed in the middle- and long-distance pool swimming events, and to develop a multivariate model that can predict the medalist group in the 10-km competition., Methods: A total of 484 athletes (252-males and 232-females) were included in the analysis. Swimmers were divided into four groups based on their finishing position in the competition. For each swimmer, the absolute best performance (PB) of 200, 400, 800 and 1500-meter in long course, the seasonal best performance (SPB) obtained before the open-water events and critical velocity (CV) were analyzed. Multivariate analysis of variance (MANOVA) was used to detect significant differences between groups and discriminant analysis was used to predict a grouping variable., Results: All the variables analyzed were significantly different between groups ( p < 0.001). The first discriminant function correctly classified 50% of the overall female and male swimmers., Conclusion: Fastest OW-swimmers have a higher speed in middle- and long-distance pool swimming events. Further studies should include different anthropometric and physiological variables to increase the accuracy of classification.

Purpose: The 10-km open-water swimming race is an endurance event that takes place in lakes, rivers, or sea and has been an Olympic event since 2008. The aim of the present brief report is to describe training volume and intensity distribution of elite open-water swimmers during the 2016 Olympic season, verifying if, in order to maximize performance, most of the training would be performed at low intensities., Methods: Eight elite Italian open-water swimmers (3 male and 5 female; 25 [2] y, 1.74 [0.05] m, 68.26 [8.17] kg) specialized in distances between 5 and 25 km participated in the study. Training load was determined using an online training diary. Training intensity was categorized according to the 3-zone model: Z1, light intensity; Z2, moderate intensity; and Z3, high intensity. Session rating of perceived exertion was used to quantify training-intensity distribution. This method assigns the entire session into a single intensity zone based on the rating of perceived exertion recorded 30 min posttraining., Results: Total yearly training volume was 3576.93 (272.390) km (3220.80-4041.97), distributed across 446 (37) (397-484) sessions monitored during the 2016 Olympic season. Training-intensity distribution in each zone was 76.83% (8.11%) in Z1, 17.70% (6.79%) in Z2, and 5.47% (5.93%) in Z3., Conclusions: High volumes in Z1 appear to be an important training method used by elite open-water swimmers. However, future research is necessary to study the effects of different training-intensity distribution on open-water swimming performances.

Resistance training (RT) is considered the most important method to improve the athlete's strength and rate of force development (RFD). In the last decade, the importance of monitoring velocity during RT has drastically grown, because of an increased availability of linear position transducers (LPT) and inertial measurement units (IMU). The purpose of this review is to analyze the existing literature on testing techniques and performance strategies used to enhance strength and power performance of elite athletes, by monitoring the velocity of resistance training. The authors focus in particular on the level of effort of resistance training defined by velocity; how the loss of velocity correlates with the degree of fatigue and how it can be used to enhance the performance of competitive athletes; the use of LPT as part of the daily routine of the strength and conditioning programs in competitive sport. It is therefore critical for the sports scientists to have a correct understanding of the basic concepts of the velocity-based training and their application to elite sports. The ultimate goal is to give some indications on the velocity-based resistance training integration in the programs of different sports in the high performance environment.

The aim of the present study was to test the effectiveness of carbohydrate (CHO) feeding supplemented every 2.5-km, as in official races, on the performance, rating of perceived exertion (RPE), and glycaemia during a 10-km intermittent training workout in elite open-water swimmers. A randomized crossover design was used. Participants completed two 10-km intermittent training sessions (20 × 500-m). The relative velocity was expressed in percentage of a single 500-m. Glycaemia was monitored by continuous glucose monitoring. Participants had to ingest either 1 L of tap water (WAT; 0.50 L·h -1 ) or 120 g of CHO in the form of 8% solution (60 g·h -1 ). The 15-point RPE scale was used during the trials. A two-way ANOVA for repeated measures was performed ( p < 0.05). The relative velocity of each 500-m was not significantly different between the two trials. No significant differences emerged in the relative velocity of the last 500-m between trials. Average RPE was not statistically different between the two trials (11 ± 3 in WAT and 12 ± 3 in CHO). In the last 500-m, glycaemia was significantly higher in the CHO trial (5.92 ± 0.47 mmol·L -1 in CHO; 5.61 ± 0.61 mmol·L -1 in WAT). CHO ingestion did not improve performance or affect RPE during a 10-km intermittent training in elite open-water swimmers.

Purpose: To analyze the pacing profiles of the world's top 800-m annual performances between 2010 and 2016, comparing men's and women's strategies., Methods: A total of 142 performances were characterized for overall race times and 0-to-200-m, 200-to-400-m, 400-to-600-m, and 600-to-800-m split times using available footage from YouTube. Only the best annual performance for each athlete was considered. Overall race and split speed were calculated so that each lap speed could be expressed as a percentage of the mean race speed., Results: The mean speed of the men's 800-m was 7.73 (0.06) m·s-1, with the 0-to-200-m split faster than the others. After the first split, the speed decreased significantly during the 3 subsequent splits (P < .001). The mean speed of the women's 800-m was 6.77 (0.05) m·s-1, with a significative variation in speed during the race (P < .001). The first split was faster than the others (P < .001). During the rest of the race, speed was almost constant, and no difference was observed between the other splits. Comparison between men and women revealed that there was an interaction between split and gender (P < .001), showing a different pacing behavior in 800-m competitions., Conclusions: The world's best 800-m performances revealed an important difference in the pacing profile between men and women. Tactics could play a greater role in this difference, but physiological and behavioral characteristics are likely also important.

Endurance exercise is associated with a transient increase in neutrophil counts in the peripheral blood. Here we investigate the impact of intensified endurance exercise on the neutrophil compartment. We hypothesized that intensified endurance exercise leads to mobilization of neutrophil subsets, which are normally absent in the blood. Furthermore, we followed the potential build-up of neutrophil activation and the impact on overnight recovery of the neutrophil compartment during a seven-day cycling tour. The neutrophil compartment was studied in 28 healthy amateur cyclists participating in an eight-day strenuous cycling tour. Blood samples were taken at baseline, after 4 days and after 7 days of cycling. The neutrophil compartment was analyzed in terms of numbers and its phenotype by deep phenotyping of flow cytometry data with the multi-dimensional analysis method FLOOD. Repeated endurance exercise led to a gradual increase in total neutrophil counts over the days leading to a 1.26 fold-increase (95%CI 1.01-1.51 p = 0.0431) in the morning of day 8. Flow cytometric measurements revealed the appearance of 2 additional neutrophil subsets: CD16brightCD62Ldim and CD16dimCD62Lbright. A complex change in neutrophil phenotypes was present characterized by decreased expression of both CD11b and CD62L and marked increased expression of LAIR-1, VLA-4 and CBRM1/5. The changes in expression were found on all neutrophils present in the blood. Strikingly, in strong contrast to our findings during acute inflammation evoked by LPS challenge, these neutrophils did not upregulate classical degranulation markers. In fact, our FLOOD analysis revealed that the exercise induced neutrophil phenotype did not overlap with the neutrophil subsets arising upon acute inflammation. In conclusion, during multiple days of endurance exercise the neutrophil compartment does not regain homeostasis overnight. Thereby our study supports the concept of a build-up of inflammatory cues during repeated endurance exercise training, causing a prolonged change of the systemic neutrophil compartment., Competing Interests: The fact that this research is part of a private public partnership TI-COAST does not alter the adherence to PLOS ONE policies on sharing data and materials.

Purpose: Reaction time has been proposed as a training monitoring tool, but to date, results are equivocal. Therefore, it was investigated whether reaction time can be used as a monitoring tool to establish overreaching., Methods: The study included 30 subjects (11 females and 19 males, age: 40.8 [10.8] years, VO 2max : 51.8 [6.3] mL/kg/min) who participated in an 8-day cycling event. The external exercise load increased approximately 900% compared with the preparation period. Performance was measured before and after the event using a maximal incremental cycling test. Subjects with decreased performance after the event were classified as functionally overreached (FOR) and others as acutely fatigued (AF). A choice reaction time test was performed 2 weeks before (pre), 1 week after (post), and 5 weeks after (follow-up), as well as at the start and end of the event., Results: A total of 14 subjects were classified as AF and 14 as FOR (2 subjects were excluded). During the event, reaction time at the end was 68 ms (95% confidence interval, 46-89) faster than at the start. Reaction time post event was 41 ms (95% confidence interval, 12-71) faster than pre event and follow-up was 55 ms faster (95% confidence interval, 26-83). The time by class interaction was not significant during (P = .26) and after (P = .43) the event. Correlations between physical performance and reaction time were not significant (all Ps > .30)., Conclusions: No differences in choice reaction time between AF and FOR subjects were observed. It is suggested that choice reaction time is not valid for early detection of overreaching in the field.

Context: Although the popularity of open-water swimming (OWS) events has significantly increased in the last decades, specific studies regarding performance of elite or age-group athletes in these events are scarce., Purpose: To analyze the existing literature on OWS., Methods: Relevant literature was located via computer-generated citations. During August 2016, online computer searches on PubMed and Scopus databases were conducted to locate published research., Results: The number of participants in ultraendurance swimming events has substantially increased in the last 10 y. In elite athletes there is a higher overall competitive level of women than of men. The body composition of female athletes (different percentage and distribution of fat tissue) shows several advantages (more buoyancy and less drag) in aquatic conditions that determine the small difference between males and females. The main physiological characteristics of open-water swimmers (OW swimmers) are the ability to swim at high percentage of [Formula: see text] (80-90%) for many hours. Furthermore, to sustain high velocity for many hours, endurance swimmers need a high propelling efficiency and a low energy cost., Conclusion: Open-water races may be characterized by extreme environmental conditions (water temperature, tides, currents, and waves) that have an overall impact on performance, influencing tactics and pacing. Future studies are needed to study OWS in both training and competition.

The aim of this study was to investigate the influence of chronotype on mood state and ratings of perceived exertion (RPE) before and in response to acute high intensity interval exercise (HIIE) performed at different times of the day. Based on the morningness-eveningness questionnaire, 12 morning-types (M-types; N = 12; age 21 ± 2 years; height 179 ± 5 cm; body mass 74 ± 12 kg) and 11 evening-types (E-types; N = 11; age 21 ± 2 years; height 181 ± 11 cm; body mass 76 ± 11 kg) were enrolled in a randomized crossover study. All subjects underwent measurements of Profile of Mood States (POMS), before (PRE), after 12 (POST12) and 24 h (POST24) the completion of both morning (08.00 am) and evening (08.00 p.m.) training. Additionally, Global Mood Disturbance and Energy Index (EI) were calculated. RPE was obtained PRE and 30 min POST HIIE. Two-way ANOVA with Tukey's multiple comparisons test of POMS parameters during morning training showed significant differences in fatigue, vigor and EI at PRE and POST24 between M-types and E-types. In addition, significant chronotype differences were found only in POST12 after the evening HIIE for fatigue, vigor and EI. For what concerns Borg perceived exertion, comparing morning versus evening values in PRE condition, a higher RPE was observed in relation to evening training for M-types ( P = 0.0107) while E-types showed higher RPE values in the morning ( P = 0.008). Finally, intragroup differences showed that E-types had a higher RPE respect to M-types before ( P = 0.002) and after 30 min ( P = 0.042) the morning session of HIIE. No significant changes during the evening training session were found. In conclusion, chronotype seems to significantly influence fatigue values, perceived exertions and vigor in relation to HIIE performed at different times of the day. Specifically, E-types will meet more of a burden when undertaking a physical task early in the day. Practical results suggest that performing a HIIE at those times of day that do not correspond to subjects' circadian preference can lead to increased mood disturbances and perceived exertion. Therefore, an athlete's chronotype should be taken into account when scheduling HIIE. Trial registration : ACTRN12617000432314, registered 24 March 2017, "retrospectively registered". Web address of trial : https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=371862&showOriginal=true&isReview=true.