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This study investigated whether repeated transitions between seated and standing positions has a different physiological response compared to continuous use of either seated position or standing position during steep uphill cycling among elite cyclists. Ten elite male cyclists completed three 5-min treadmill cycling tests at an inclination of 6.8° with constant individual-based speed resulting in a work intensity close to the aerobic threshold. During the first and third test, the participants used standing position (ST test) and seated position (SE test) or vice versa, whereas in the second test, they made repeated transitions between standing and seated positions every 10 s (RT test). The last 2 min of each test was used to measure the mean values of oxygen uptake (V̇O2) and respiratory exchange ratio, which were used to calculate the metabolic rate (MR) and gross efficiency (GE). Additionally, the blood-lactate concentration before and after (Lapost) each test was determined. One-way repeated measures ANOVA was used to determine the effect of cycling position on the physiological response. No significant differences between tests were observed for the variables related to aerobic energy expenditure (i.e., V̇O2, MR and GE), whereas the RT test was associated with a significantly lower Lapost compared to the ST and SE tests. Steep uphill cycling, at an intensity close to the aerobic threshold, with repeated transitions between standing and seated positions, did not have a higher oxygen consumption; instead, the blood-lactate concentration was lower during the RT test compared to that under continuous use of either seated or standing position. [ABSTRACT FROM AUTHOR]

To better cope with stress in emergencies, emergency personnel undergo virtual reality (VR) stress training. Such training typically includes visual, auditory and sometimes tactile impressions, whereas olfactory stimuli are mostly neglected. This concept paper therefore examines whether odors might be beneficial for further enhancing the experience of presence and immersion into a simulated environment. The aim is to demonstrate the benefits of VR civilian stress training for emergency personnel and to investigate the role of odors as stressors by manipulating the degree of perceived psychophysiological stress via olfactory impressions. Moreover, the current paper presents the development and validation of a convenient and portable fragrance dosing system that allows personalized odor presentation in VR. The presented system can transport reproducible small quantities of an air-fragrance mixture close to the human nose using piezoelectric stainless steel micropumps. The results of the fluidic system validation indicate that the micropump is suitable for releasing odors close to the nose with constant amounts of odor presentation. Furthermore, the theoretical background and the planned experimental design of VR stress training, including odor presentation via olfactory VR technology, are elucidated. [ABSTRACT FROM AUTHOR]

This study investigated how different relative humidity (RH) would impact behavior and physiology of laying hens. One hundred-eighty laying hens (Hy-Line Brown), aged 68-week-old (1,908 ± 78 g), were exposed to daily ambient temperatures of 30 °C with RH set at 25% (RH25), 50% (RH50), or 75% (RH75) for 12 h a day (9:00 am to 9:00 pm) from 70 to 74 weeks of age. All hens had been raised at 24 °C and 50% RH during 2-week adaptation period. Each RH treatment consisted of 10 replicates with 6 hens each in a completely randomized design. Results indicated that rectal temperature, respiratory rate, and heart rate were increased (P ≤ 0.05) in the RH75 group compared with the RH25 and RH50 groups. Plasma malondialdehyde concentrations were elevated in the RH75 group compared RH50 at 3, 7, and 21 days (P ≤ 0.05) following the RH exposure. Plasma glutathione peroxidase activity was lowered (P ≤ 0.05) in the RH75 group compared with the RH25 and RH50 groups at 21 days. Increasing RH led to decreased feeding behavior but showed a tendency to increase drinking behavior. Wings elevated and panting behaviors were higher in the RH75 group compared with the RH25 and RH50 groups (P ≤ 0.05). In conclusion, these findings suggest that elevated RH at constant ambient temperature could negatively influence the behavior and physiology of laying hens. [ABSTRACT FROM AUTHOR]

Activity and physical effort positively affect a person's psychophysical state and emotional experience. Interest in the phenomenon of flow, the state of perceived arousal, stems from its relationship to an individual's intrinsic motivation. Flow is an area of research in many fields, including sports. Nowadays, solutions are being sought to support the traditional assessment of cognitive and affective states using analysis of physiological signals. Therefore, the present study analysed and estimated the physiological responses that may occur during the induction of a flow state between exercises stimulated by additional metrorhythmic stimuli. Thirty-six healthy subjects participated in the study. The effects of various metrorhythmic stimuli on the body's physiological response during the subjects' free gait were examined. The physiological response and flow intensity were evaluated when the rate of individual stimuli was changed, and the rate was enforced. Several statistically significant variables and correlations were determined for physiological indicators depending on the stage of the study conducted and the level of flow experienced. A positive, statistically significant correlation of flow and HRV frequency variables was obtained. The results also confirm previous literature reports on the relationship between flow response and frequency heart rate variability during physical activity. [ABSTRACT FROM AUTHOR]

The circular aquaculture model of largemouth bass pond engineering has the characteristics of high yield and efficiency, but it is prone to stress caused by flow velocity and density, which affects the yield of largemouth bass (Micropterus salmoides). γ‐Aminobutyric acid (GABA) is believed to have the effect of improving growth and stress tolerance. We divided the largemouth bass into three groups: a control group, a flow rate and density combined stress group, and a combined stress feed supplemented with GABA (0.9%) group, and conducted a 60‐day aquaculture experiment. The results showed that the final weight, weight gain rate (WGR), specific growth rate (SGR), and feed efficiency (FE) of largemouth bass significantly decreased in the combined stress group (P < 0.05). The serum aspartate aminotransferase (AST), alanine transaminase (ALT) activity, and glucose (GLU), malondialdehyde (MDA) level of largemouth bass significantly higher than the control group, and the serum lysozyme (LZM) activity and total antioxidant capacity (T‐AOC) were significantly lower than the control group (P < 0.05). After adding GABA, the final weight, WGR, SGR, and FE decreased, and the serum GLU levels, AST, ALT activity, and MDA levels were downregulated, and the serum LZM activity and T‐AOC of largemouth bass were upregulated. But most of the above are still at the level of the control group. Under combined stress, the messenger ribonucleic acid (mRNA) expression levels of growth hormone (GH), insulin‐like growth factor‐Ⅰ (IGF‐I), B‐cell lymphoma‐2 (Blc2), nuclear transcription factor E2‐related factor 2 (Nrf2), catalase (CAT), and superoxide dismutase (SOD) genes were significantly reduced (P < 0.05), while the mRNA expression levels of heat stress protein 70 (HSP70), heat stress protein 90 (HSP90), nuclear factor kappa‐B (NF‐κB), interleukin‐1β (IL‐1β), Bax and keap1 genes were significantly increased (P < 0.05). After the exogenous addition of GABA, all the above genes have a certain degree of callback, but GH, HSP70, HSP90, IL‐1β, Bax, Nrf2, CAT, and SOD have not yet reached the level of the control group. These results indicate that adding GABA to feed can alleviate the adverse effects of combined stress of flow rate and density to a certain extent and provide insights for solving the problems in the circular aquaculture model of largemouth bass. [ABSTRACT FROM AUTHOR]

Ligusticum sinense cv. Chuanxiong (L. Chuanxiong), one of the widely used traditional Chinese medicines (TCM), is currently facing the problem of excessive cadmium (Cd) content. This problem has significantly affected the quality and safety of L. Chuanxiong and become a vital factor restricting its clinical application and international trade development. Currently, to solve the problem of excessive Cd, it is essential to research the response mechanisms of L. Chuanxiong to Cd stress. However, there are few reports on its physiological and biochemical responses under Cd stress. In this study, we conducted the hydroponic experiment under 25 μM Cd stress, based on the Cd content of the genuine producing areas soil. The results showed that 25 μM Cd stress not only had no significant inhibitory effect on the growth of L. Chuanxiong seedlings but also significantly increased the chlorophyll a content (11.79%) and root activity (51.82%) compared with that of the control, which might be a hormesis effect. Further results showed that the absorption and assimilation of NH4+ increased in seedlings under 25 μM Cd stress, which was associated with high photosynthetic pigments. Here, we initially hypothesized and confirmed that Cd exceedance in the root system of L. Chuanxiong was due to the thickening of the root cell wall, changes in the content of the cell wall components, and chelation of Cd by GSH. There was an increase in cell wall thickness (57.64 %) and a significant increase in cellulose (25.48%) content of roots under 25 μM Cd stress. In addition, L. Chuanxiong reduced oxidative stress caused by 25 μM Cd stress mainly through the GSH/GSSG cycle. Among them, GSH-Px (48.26%) and GR (42.64%) activities were significantly increased, thereby maintaining a high GSH/GSSG ratio. This study preliminarily reveals the response of L. Chuanxiong to Cd stress and the mechanism of Cd enrichment. It provides a theoretical basis for solving the problem of Cd excessive in L. Chuanxiong. Physiological and biochemical mechanisms of L. Chuanxiong seedlings under Cd stress. Highlights: 1. Under 25 μM Cd, it was the hormesis effect on the growth of L. Chuanxiong. 2. Seedlings increased the thickness of root cell wall to chelate and fixate Cd. 3. Seedlings enhanced NH4+ absorption and assimilation, thereby increasing chlorophyll content. 4. Seedlings mainly reduced oxidative stress caused by Cd stress through the GSH/GSSG cycle. [ABSTRACT FROM AUTHOR]

Animals spend a considerable proportion of their life span at rest. However, resting status has often been overlooked when investigating how species respond to environmental conditions. This may induce a large bias in understanding the local adaptation of species across environmental gradients and their vulnerability to potential environmental change. Here, we conducted an empirical study on montane agamid lizards, combined with mechanistic modeling, to compare elevational variations in body temperature and metabolisms (cumulative digestion and maintenance cost) between resting and active status. Our study on three populations of an agamid lizard along an elevational gradient revealed a trend of decreasing body temperature toward higher elevations, the main contributor of which was resting status of the lizards. Using population‐specific reaction norms, we predicted greater elevational variation in hourly and cumulative digestion for resting lizards than for active lizards. Climate‐change impacts, estimated as the change in cumulative digestion, also show greater elevational variation when resting status is factored into the analysis. Further, our global analysis of 98 agamid species revealed that in about half of their combined distributional range, the contribution of resting status in determining the elevational variation in cumulative digestion and maintenance cost of lizards was greater than the contribution made by a lizard's active status. Our study highlights the importance of considering resting status when investigating how species respond to environmental conditions, especially for those distributed over tropical and subtropical mountain areas. [ABSTRACT FROM AUTHOR]

Organic farming is gaining recognition as a viable alternative to conventional methods, promising soil health preservation and sustained crop productivity with economic benefits. This study evaluated the physiological, growth, and yield responses of the PSB Rc18 rice variety and appraised its economic feasibility under different production systems. The experiment was laid out in Randomized Complete Block Design (RCBD) with four replications and three treatments: T1-best bet organic production system, T2-farmers' organic production system in Leyte, and T3-farmers' conventional production system in Leyte. The crop growth rate (CGR) of PSB Rc18 remained consistent across the different systems. However, the Net Assimilation Rate (NAR) peaked significantly between 42-56 days after transplanting (DAT) in the T2. Additionally, the Leaf Area Index (LAI) in T1 was comparable to that of T3. Rice grown under T1 reached heading and maturation earlier than T3. Although T3 produced the highest fresh straw, most productive tillers, and heaviest total biomass, the grain yield was similar across all production systems. Economically, T2 outperformed with a superior benefit-cost ratio of $0.55 and $0.94 per USD invested, considering both regular and premium prices for organic palay. These findings highlight organic farming practices' economic and agronomic viability, suggesting that promoting organic farming can be a beneficial alternative to conventional methods in Leyte. This study underscores the potential for integrating organic practices to enhance sustainability and economic outcomes in rice production, making both T1 and T2 significant options for farmers in Eastern Visayas. [ABSTRACT FROM AUTHOR]

Drought stress, as one of the most common environmental factors, seriously affects seed- ling establishment as well as plant growth and productivity. The growth of Toona ciliata is constrained by soil moisture deficit, and drought stress can reduce its productivity and limit its suitable growing environment. To explore the molecular mechanism of Toona ciliata responding to drought stress, leaves of two-year-old Toona ciliata seedlings were used as experimental materials for transcriptome sequencing and physiological index measurements. Under drought stress, the contents of Chl, MDA, POD, SP, SS, and RWC all change differently. We performed transcriptome sequencing, obtaining 4830 differential genes. The enrichment analysis indicates that the primary effects on the leaves of Toona ciliata under drought stress are related to photosynthesis and responses to plant hormone signal transduction. Transcription factor families associated with drought resistance include the NAC, WRKY, bZIP, bHLH, AP2-EREBP, C3H, GRAS, and FRAI transcription factor families. A weighted gene co-expression network analysis (WGCNA) analysis successfully identified 10 hub genes in response to drought stress in Toona ciliata leaves. Real-time quantitative PCR (RT-qPCR) validated the reliability of the transcriptomic data, and the analysis of its results showed a close correlation with the data obtained from RNA-seq. This study clarifies the transcriptional response of Toona ciliata to drought stress, contributing to the revelation of the molecular mechanisms of drought adaptation. [ABSTRACT FROM AUTHOR]

Musculoskeletal Disorders (MSDs) significantly threaten the health and well-being of farmers who carry head loads. Despite the prevalence of MSDs, a major barrier to preventing this issue is the lack of awareness and training among farmers. In Mantalongon, Dalaguete, Cebu, vegetables are predominantly transported by head-load carriages, exposing farmers to substantial health risks. This study aimed to evaluate the perceptual and physiological responses of farmers to identify risk factors associated with head-load carrying. The research involved 36 farmers, examining the relationship between MSDs and various task-related variables, health history, and personal profiles. The results indicated that the farmers rated their work intensity as "moderate" on the Borg scale and could carry loads averaging 147% of their body weight. Despite these strenuous conditions, no significant statistical correlation was found between personal profiles, health histories, and reported pain and discomfort. Four primary risk factors were identified: (i) the load weight carried significantly affected the Rating of Perceived Exertion (RPE) (p=0.003), neck discomfort (p=0.002), upper back discomfort (p=0.001), and heart rate variation (p=0.025), (ii) the terrain traveled was associated with upper back discomfort (p=0.025), lower back discomfort (p=0.046), and leg discomfort (p=0.031), (iii) the distance traveled significantly influenced heart rate variation (p=0.001), and (iv) the lifting technique was correlated with lower back discomfort (p=0.023). These findings underscore the urgent need for targeted interventions to mitigate MSD risks among farmers. Implementing comprehensive awareness and training programs can provide farmers with the necessary knowledge and skills to reduce the physical strain associated with carrying head loads, thus improving their health and overall well-being. [ABSTRACT FROM AUTHOR]

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Maritime studies, encompassing a range of disciplines, increasingly rely on advanced data analytics, particularly in the context of navigation. As technology advances, the statistical averaging of large datasets has become a critical component of these analyses. However, recent studies have highlighted discrepancies between statistical predictions and observable realities, especially in high-stress environments like port approach procedures conducted by marine pilots. This study analyzed physiological responses recorded during simulation exercises involving experienced marine pilots. The focus was not on the specific outcomes of the simulations but on the potential faults arising from conventional statistical signal processing, particularly mean-centered approaches. A large dataset of signals was generated, including one signal with a dominant characteristic intentionally designed to introduce imbalance, mimicking the uneven distribution of real-world data. Initial analysis suggested that the average physiological response of the pilots followed an S-shaped curve, indicative of a psychophysiological reaction to stress. However, further post hoc analysis revealed that this pattern was primarily influenced by a single participant's data. This finding raises concerns about the generalizability of the S-curve as a typical stress response in maritime pilots. The results underscore the limitations of relying solely on conventional statistical methods, such as mean-centered approaches, in interpreting complex datasets. The study calls into question the validity of standardizing data interpretations based on dominant characteristic curves, particularly in environments as intricate as maritime navigation. The research highlights the need for a re-evaluation of these methods to ensure more reliable and nuanced conclusions in maritime studies. This study contributes to the ongoing discourse on data interpretation in maritime research, emphasizing the critical need to reassess conventional statistical signal processing techniques. By recognizing the potential pitfalls in data generalization, the study advocates for more robust analytical approaches to better capture the complexities of realworld maritime challenges. [ABSTRACT FROM AUTHOR]

The potential of Euglena gracilis for carbon sequestration offers significant opportunities in the capture and utilization of carbon dioxide (CO2 ). In this study, a mutant LE-ZW of E. gracilis, capable of efficient growth and carbon sequestration, was obtained through ultraviolet mutagenesis combined with high carbon acclimation. Subsequently, the potential of LE-ZW for carbon assimilation was systematically analyzed. The results demonstrated that the cell density of the LE-ZW was 1.33 times that of the wild type and its carbon sequestration efficiency was 6.67 times that of the wild type when cultured at an optimal CO2 concentration of 5% until day 10. At this time, most key enzyme genes associated with the photosystem membrane protein complex, photosynthetic electron transport chain, antenna protein, and carbon fixation were up-regulated in mutant LE-ZW. Furthermore, after 10  days of culture under 10% CO2, the cell density and carbon sequestration efficiency of LE-ZW reached 1.10 times and 1.54 times of that under 5% CO2, respectively. Transcriptome analysis revealed significant up-regulation of key enzyme genes associated with carbon fixation, central carbon metabolism, and photosynthesis in LE-ZW under a 10% CO2 concentration. Physiological indices such as the amount of oxygen evolution, the values of Fv/Fm, the expression levels of photosynthetic protein genes and the enzyme activity of key enzymes related to photosynthetic carbon assimilation were corroborated by transcriptome data, elucidating that the mutant LE-ZW exhibited augmented photosynthetic carbon sequestration capacity and metabolic activity, thereby demonstrating robust adaptability to a high-carbon environment. This research contributes to a deeper understanding of the carbon assimilation mechanism in photosynthetic protists under elevated CO2 concentrations. [ABSTRACT FROM AUTHOR]

Plant biological and physiological ecological characteristics are changed by cultivation and domestication, but little is known about the responses of medicinal plant to heavy metal pollution. The effects of Cd addition on biological characters, Cd accumulation and physiological response of Erigeron breviscapus were studied. In this study, pot culture was conducted with four Cd concentration gradients of 0, 2.5, 5, 15 mg·kg-1, meanwhile, the tolerances of two types of Erigeron breviscapus to cadmium were evaluated by membership function method. Results showed that the increase of soil Cd concentration increased significantly the Cd content of Erigeron breviscapus but tended to decrease plant height and SPAD value. However, the leaf length and width, soluble protein and MDA of Erigeron breviscapus varied slightly with increasing of Cd concentration in soil. At higher Cd concentration (15 mg·kg-1), the biomass of two types of Erigeron breviscapus was significantly higher than that without Cd addition treatment, while SPAD value of wild type was significantly higher than cultivated type. There were no significant differences in activities of SOD, POD, CAT and contents of GSH, NPT, PCS between the two types of Erigeron breviscapus at the same concentration of Cd. The Cd tolerance index, average enrichment coefficient and transfer coefficient of both two types Erigeron breviscapus was greater than 1, 0.5 and 0.5, respectively, but those index and coefficient were not significantly different between two types. Cultivated and wild type of Erigeron breviscapus had strong Cd enrichment ability and Cd tolerance, the cultivated Erigeron breviscapus belonged to medium Cd tolerance type, while wild type belonged to high Cd tolerance type. After cultivation and domestication, the Cd tolerance ability of Erigeron breviscapus decreased, but transfer and enrichment ability did not change significantly. [ABSTRACT FROM AUTHOR]

[Objectives] his study aimed to explore the effects of drought stress on morphological changes, photosynthetic characteristics and other physiological indexes of ginger at seedling stage, and to provide reference for the study of drought tolerance mechanism and the selection of drought-tolerant varieties of ginger. [Methods] Five main varietie (‘Shandongdajiang'‘Fengtoujiang'‘Zhugenjiang'‘Luopingxiaohuangjiang'and‘Guizhouxiaohuangjiang') were selected as test materials from the main ginger producing areas of North China, Central China and Southwest China. Drought stress was simulated by 250 g·L-1 PEG-6000 solution, normal water treatment (CK) and drought treatment (DS) was set to determine the growth and physiological indexes such as leaf moisture content, leaf water potential, photosynthesis, stomatal phenotype, chlorophyll content and other growth and physiological indexes of different varieties of ginger under different treatments at 15 days, and the lignin deposition of ginger stems was observed by paraffin sectioning method. [Results] Compared with CK, the leaves of various ginger varieties had different degrees of curly yellowness at 15 days of drought treatment. The free water content (FWC), relative water content (RWC) and total water content (TWC) of leaves decreased significantly, the content of bound water (BWC) of ‘Shandongdajiang' increased, and the BWC content of the remaining four varieties decreased significantly. The water potential of the leaves of various varieties of ginger decreased significantly, and the ‘Fengtoujiang' decreased the most. In addition, the net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), transpiration rate (Tr) of leaves decreased, the length and width of the stomata decreased, and the length and width of guard cells did not change significantly. Among the five varieties, the photosynthetic index of ‘Luopingxiaohuangjiang' was the most affected, and the stomatal conductance and transpiration rate decreased by 100% and 93.84%, respectively. The contents of chlorophyll a (Chla), chlorophyll b (Chlb) and total chlorophyll a+b (Chla+b) decreased but not significantly, while the content of carotenoid (Car) increased in‘Shandongdajiang 'but not significantly, and decreased significantly in the other four varieties. After drought treatment, the amount of stem lignin deposition increased in‘Shandongdajiang 'and‘ Luopingxiaohuangjiang'. [Conclusions] Drought stress affected the growth and development of ginger seedlings, and there were differences in drought tolerance among different ginger varieties. The drought tolerance of the five ginger varieties was ranked from strong to weak as follows: ‘Shandongdajiang'‘Fengtoujiang'‘Guizhouxiaohuangjiang'‘Zhugenjiang'‘Luopingxiaohuangjiang'.‘Shandongdajiang'had strong drought tolerance, which could be used for further analysis of the molecular mechanism of drought resistance and creation of drought resistance breeding high-quality germplasm materials. [ABSTRACT FROM AUTHOR]

This study simulated the effects of direct predatory pressure, indirect predatory pressure, and conspecific injury signals on sea cucumber (Apostichopus japonicus) to determine changes in the activity of immune defense enzymes (lysozyme, acid phosphatase, alkaline phosphatase) and antioxidant stress enzymes (catalase, superoxide dismutase, malondialdehyde). Samples of sea cucumber juveniles were collected at 3 h, 12 h, 72 h, and 96 h post predatory stress, and six enzymes related to immune defense and antioxidant stress were selected for activity assays, namely, lysozyme (LZM), acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA). The results indicate that under direct predatory pressure, the activity of catalase in sea cucumbers was significantly higher than that of the control group at 3 h (P < 0.05), while the activities of acid phosphatase, alkaline phosphatase, and catalase were significantly lower at 72 h (P < 0.05). Under indirect predatory pressure, the activity of malondialdehyde in sea cucumbers was significantly higher than that of the control group at 12 h (P < 0.05), the activity of alkaline phosphatase was significantly higher at 72 and 96 h (P < 0.05), the activity of catalase was significantly lower at 72 h, and the activity of superoxide dismutase was significantly higher at 72 h (P < 0.05). Under the influence of conspecific injury signals, the activity of malondialdehyde in sea cucumbers was significantly higher than that of the control group at 12 h (P < 0.05), and the activity of superoxide dismutase was significantly higher at 96 h (P < 0.05). The sea cucumber enhanced its antioxidant capacity 3 h after facing a predator, while its immune defense mechanism was suppressed at 72 h. When facing indirect predatory pressure, the sea cucumber may have made immune and antioxidant preparations for the arrival of unknown risks. The experimental results show that predatory pressure has a significant impact on the immune and antioxidant functions of sea cucumbers, which may be related to the physiological state and environmental adaptability of the sea cucumber. This study provides a new perspective for understanding how sea cucumbers cope with predatory pressure in the natural environment and offers theoretical support for the cultivation management of sea cucumbers. [ABSTRACT FROM AUTHOR]

Drawing inspiration from the recent trend of Sauce-Flavored Latte, this paper ingeniously explores the dual narrative of "wine meets coffee", unraveling the anthropomorphic tale of ethanol and caffeine. Delving into their chemical properties, physiological mechanisms, and metabolic pathways, the narrative aims to enlighten readers about the underlying chemistry and health implications, enabling a more informed and moderate consumption of beverages. [ABSTRACT FROM AUTHOR]

Denphal-type Dendrobium is the famous cut and potted flower in the world, and most cultivated in tropical and subtropical regions. However, it often suffers from cold in winter in subtropical regions. To verify the physiological response of Denphal-type Dendrobium under low temperature in this study, the mature and young plantlets of Dendrobium Udomsri Beauty were treated under 15, 10, and 5 °C, respectively. And then the electrical conductivity (EC), soluble protein, soluble sugar, free proline, malondialdehyde (MDA), chlorophyll content, and the rate of defoliation after regrowth were measured. The results showed that both mature plant and young seedlings of Dendrobium Udomsri Beauty, the EC, soluble protein, soluble sugar, free proline, MDA content, and defoliation rate were increased with the decrease of treatment temperature and the extension of treatment times. The content of chlorophyll decreased gradually with the decrease of treatment temperature and the extension of treatment times. The correlation analysis showed that soluble sugar, free proline, MDA, chlorophyll content, and defoliation rate were significantly correlated with the semi-lethal temperature. It is indicated that the content of free proline, MDA, chlorophyll, and defoliation rate could be used as the effective indexes for the comprehensive assessment of cold tolerance of Dendrobium Udomsri Beauty. [ABSTRACT FROM AUTHOR]

Water and nitrogen are widely used agricultural inputs in potato production areas. However, the excessive and inappropriate use of these significant inputs may lead to the rapid depletion of water resources and inappropriate nitrogen leakage. In potato production, insight into the effects of water and nitrogen on the physiological properties will help to overcome these difficulties. This study was therefore conducted to determine the effects of different nitrogen and irrigation levels on the physiological (photosynthesis rate, leaf area index, leaf chlorophyll content, and leaf temperature) characteristics of potato. Field experiments were conducted during the 2021–2022 and 2022–2023 primary potato cropping seasons to select the suitable combination of irrigation and fertilization levels for potato production in the Nigde Province of the Central Anatolia Region of Turkey. Employing a factorial design, the study encompassed six nitrogen fertilization rates and three irrigation levels. Measurements were systematically recorded at five distinct intervals (40, 50, 60, 70, and 80 days) post-emergence. The outcomes underscored that nitrogen treatments augmented physiological traits, manifesting in heightened photosynthetic rates, chlorophyll content, and leaf area indices, barring leaf temperature, in comparison to the control group (devoid of nitrogen treatments). Full irrigation (I1) yielded the utmost values for both photosynthesis and leaf area index, with I1N3 (full irrigation and intermediate nitrogen supply) was identified as the most efficacious treatment for these parameters. This study identifies the most appropriate nitrogen and irrigation application for optimum physiological yield of the potato for crop producers. However, it is important to note that the study focused solely on one commercial potato cultivar (Agria), and further research on various potato cultivars is recommended for more conclusive results. [ABSTRACT FROM AUTHOR]

Cadmium (Cd) pollution has emerged as a critical global environmental concern, due to its significant toxicity, environmental persistence, and the pervasiveness of contamination. Significantly, the bioaccumulation of Cd in agricultural crops constitutes a primary vector for its entry into the human diet. This issue warrants urgent attention from both the scientific community and policymakers to develop and implement effective mitigation strategies. This review delves into the physiological impacts of Cd stress on plants, including the suppression of photosynthetic activity, amplification of oxidative stress, and disruptions in mineral nutrient homeostasis. Additionally, the resistance mechanisms deployed by plants in response to Cd stress have been explored, and the prospective contributions of molecular breeding strategies in augmenting crop tolerance to Cd and minimizing its bioaccumulation have been assessed. By integrating and analyzing these findings, we seek to inform future research trajectories and proffer strategic approaches to enhance agricultural sustainability, safeguard human health, and protect environmental integrity. [ABSTRACT FROM AUTHOR]

Background and Study Aim Monitoring physiological responses during training is crucial for understanding how athletes adapt to various exercise intensities. However, the specific effects of combining Small-Sided Game (SSG) and Speed-Endurance Training (SET) on these parameters require the search for more effective solutions. This study aims to determine the effect of combined SSG and SET on heart rate (HR) and rate of perceived exertion (RPE). Material and Methods This quantitative research employed an experimental method with a weekly pretest-posttest design. The study population consisted of eighty-two members of the Unesa Soccer Student Activity Unit. A sample of eighteen individuals was selected through simple random sampling. The sample had the following characteristics: age 20±0.69 years, weight 65±9.54 kg, height 165.22±4.31 cm, and BMI 22.82±1.92. Data were collected using the Polar H-10 device, and RPE interviews were conducted ten minutes after each training session. Data analysis was performed using paired sample t-tests and Wilcoxon tests. Results The results showed a significant difference in HR for pair 1 (p = 0.037), but no significant differences for pair 2 (p = 0.058) or pair 3 (p = 0.076). However, the results for RPE indicated significant differences in pair 1 (p = 0.001), pair 2 (p = 0.004), and pair 3 (p = 0.002). The combination of SSG and SET led to an increase in HR during the first and second weeks. In the third week, HR decreased. RPE increased from the first to the second week. It then stabilized in the third and fourth weeks, indicating a consistent level of effort. The decrease in HR during the third week, despite the same effort level, suggests that the athletes successfully adapted to the training program. Conclusions The combination of SSG and SET demonstrates potential as an effective training method for improving both physiological performance and adaptation in athletes. These findings offer valuable insights into how athletes respond to structured training programs, providing a foundation for future research and practical applications in sports training. The results contribute to a deeper understanding of the role that specific training combinations play in athlete development and recovery. [ABSTRACT FROM AUTHOR]

The design of vehicle cabin seats is crucial in transportation, as it directly affects the safety and comfort of both drivers and passengers. To design seat parameters that enhance sitting comfort, a quantitative evaluation of sitting comfort involving an understanding of users' physiological responses is necessary. This study aimed to assess users' physiological responses to relaxation induced by changes in seat parameters using electroencephalography and electrocardiography. We examined the physiological responses and subjective evaluations of relaxation in fifteen participants, focusing on the effects of reclining, ottoman, and slab. The results demonstrated an improvement in the subjective level of relaxation with changes in all seat parameters set here. However, central nervous system responses and autonomic nervous system reactions varied based on alterations in posture angles and seat pressure distributions. This underscores the importance of physiological markers, encompassing indicators of autonomic and central nervous system responses, in evaluating relaxation in relation to changes in posture angles and seat pressure distribution. [ABSTRACT FROM AUTHOR]

Genetic transformation of forest trees is essential for validating gene functions and breeding new varieties through molecular means. Appropriate selective pressure is critical for creating an effective screening system. 'Beilinxiongzhu 1' sensitivity testing showed that the critical tolerance concentrations for hygromycin (Hyg), kanamycin (Kan), and glyphosate (PPT) in leaf explants were 2.0 mg/L, 20 mg/L, and 1.0 mg/L, respectively. Among the physiological indicators, soluble sugar content, soluble protein content, and endogenous hormone levels were identified as key markers of the effects of the different antibiotic treatments. Transcriptome analysis showed that Hyg treatment resulted in a large number of differentially expressed genes (DEGs) involved in leaf cell wall synthesis and glucose metabolism. Under Kan treatment, the DEGs were associated with pathways such as ribosome biosynthesis and histone packaging in eukaryotes. Under PPT treatment, significant DEGs were related to ABC transporters. DEGs common to all three antibiotics were involved in glutathione metabolism pathways. A weighted gene co-expression network analysis identified TRXH2, H3.2, H2B, GST, U71K1, and CHS as key genes in response to antibiotic stress. By elucidating the physiological and molecular mechanisms by which different antibiotics affect leaf sprouting, our study serves as a reference for research into the genetic transformation of poplar leaves. [ABSTRACT FROM AUTHOR]

Background and Study Aim. Monitoring physiological responses during training is crucial for understanding how athletes adapt to various exercise intensities. However, the specific effects of combining Small-Sided Game (SSG) and Speed-Endurance Training (SET) on these parameters require the search for more effective solutions. This study aims to determine the effect of combined SSG and SET on heart rate (HR) and rate of perceived exertion (RPE). Material and Methods. This quantitative research employed an experimental method with a weekly pretest-posttest design. The study population consisted of eighty-two members of the Unesa Soccer Student Activity Unit. A sample of eighteen individuals was selected through simple random sampling. The sample had the following characteristics: age 20±0.69 years, weight 65±9.54 kg, height 165.22±4.31 cm, and BMI 22.82±1.92. Data were collected using the Polar H-10 device, and RPE interviews were conducted ten minutes after each training session. Data analysis was performed using paired sample t-tests and Wilcoxon tests. Results. The results showed a significant difference in HR for pair 1 (p = 0.037), but no significant differences for pair 2 (p = 0.058) or pair 3 (p = 0.076). However, the results for RPE indicated significant differences in pair 1 (p = 0.001), pair 2 (p = 0.004), and pair 3 (p = 0.002). The combination of SSG and SET led to an increase in HR during the first and second weeks. In the third week, HR decreased. RPE increased from the first to the second week. It then stabilized in the third and fourth weeks, indicating a consistent level of effort. The decrease in HR during the third week, despite the same effort level, suggests that the athletes successfully adapted to the training program. Conclusions. The combination of SSG and SET demonstrates potential as an effective training method for improving both physiological performance and adaptation in athletes. These findings offer valuable insights into how athletes respond to structured training programs, providing a foundation for future research and practical applications in sports training. The results contribute to a deeper understanding of the role that specific training combinations play in athlete development and recovery.

Abstract Ligusticum sinense cv. Chuanxiong (L. Chuanxiong), one of the widely used traditional Chinese medicines (TCM), is currently facing the problem of excessive cadmium (Cd) content. This problem has significantly affected the quality and safety of L. Chuanxiong and become a vital factor restricting its clinical application and international trade development. Currently, to solve the problem of excessive Cd, it is essential to research the response mechanisms of L. Chuanxiong to Cd stress. However, there are few reports on its physiological and biochemical responses under Cd stress. In this study, we conducted the hydroponic experiment under 25 μM Cd stress, based on the Cd content of the genuine producing areas soil. The results showed that 25 μM Cd stress not only had no significant inhibitory effect on the growth of L. Chuanxiong seedlings but also significantly increased the chlorophyll a content (11.79%) and root activity (51.82%) compared with that of the control, which might be a hormesis effect. Further results showed that the absorption and assimilation of NH4 + increased in seedlings under 25 μM Cd stress, which was associated with high photosynthetic pigments. Here, we initially hypothesized and confirmed that Cd exceedance in the root system of L. Chuanxiong was due to the thickening of the root cell wall, changes in the content of the cell wall components, and chelation of Cd by GSH. There was an increase in cell wall thickness (57.64 %) and a significant increase in cellulose (25.48%) content of roots under 25 μM Cd stress. In addition, L. Chuanxiong reduced oxidative stress caused by 25 μM Cd stress mainly through the GSH/GSSG cycle. Among them, GSH-Px (48.26%) and GR (42.64%) activities were significantly increased, thereby maintaining a high GSH/GSSG ratio. This study preliminarily reveals the response of L. Chuanxiong to Cd stress and the mechanism of Cd enrichment. It provides a theoretical basis for solving the problem of Cd excessive in L. Chuanxiong. Graphical Abstract Physiological and biochemical mechanisms of L. Chuanxiong seedlings under Cd stress.

Organic farming is gaining recognition as a viable alternative to conventional methods, promising soil health preservation and sustained crop productivity with economic benefits. This study evaluated the physiological, growth, and yield responses of the PSB Rc18 rice variety and appraised its economic feasibility under different production systems. The experiment was laid out in Randomized Complete Block Design (RCBD) with four replications and three treatments: T1-best bet organic production system, T2-farmers’ organic production system in Leyte, and T3-farmers’ conventional production system in Leyte. The crop growth rate (CGR) of PSB Rc18 remained consistent across the different systems. However, the Net Assimilation Rate (NAR) peaked significantly between 42-56 days after transplanting (DAT) in the T2. Additionally, the Leaf Area Index (LAI) in T1 was comparable to that of T3. Rice grown under T1 reached heading and maturation earlier than T3. Although T3 produced the highest fresh straw, most productive tillers, and heaviest total biomass, the grain yield was similar across all production systems. Economically, T2 outperformed with a superior benefit-cost ratio of $0.55 and $0.94 per USD invested, considering both regular and premium prices for organic palay. These findings highlight organic farming practices' economic and agronomic viability, suggesting that promoting organic farming can be a beneficial alternative to conventional methods in Leyte. This study underscores the potential for integrating organic practices to enhance sustainability and economic outcomes in rice production, making both T1 and T2 significant options for farmers in Eastern Visayas.

Pre-weaning growth is crucial for achieving high weaning weights, which are expected to positively correlate with the overall performance of does/ewes. Milk replacer, designed to meet the nutritional needs of pre-weaning kids, offers a solution to this challenge. This study aimed to analyze and evaluate the impact of incorporating maggot flour into milk replacer on the performance of pre-weaned Peranakan Etawa (PE) crossbreed goats. The parameters observed included milk replacer intake, nutrient consumption, body weight gain, weaning weight, rectal temperature, respiratory rate, and feed cost per gain. The study involved 10 two-week-old local pre-weaned PE goats, each with an average body weight of 5.33 ± 0.93 kg, divided into two treatment groups with five replicates each. The treatments included a commercial milk replacer (P0) and a milk replacer containing maggot flour (P1). Data were analyzed using an independent t-test at a 5% significance level. The results showed that daily body weight gain in pre-weaned PE goats fed milk replacer containing maggot flour did not differ significantly (P0.05) from those fed the commercial milk replacer. The physiological responses of the pre-weaned goats, including rectal temperature and respiratory rate, were similar between treatments and did not negatively affect their physiological status. The lowest feed cost per gain was observed in the group fed the maggot flour-based milk replacer (P1), indicating its potential economic advantage.

This study investigated the effects of varying dietary protein and lipid levels on the growth performance and whole-body composition of climbing perch (Anabas testudineus) fry. Fish (initial weight: 0.34 ± 0.01 g) were fed nine semi-purified diets with three protein (35%, 40%, and 45%) and three lipids (6%, 8%, and 10%) levels for 60 days. While survival rates were unaffected by dietary treatments, fish fed a diet containing 40% protein and 8% lipid exhibited significantly higher (P < 0.05) weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER), and protein productive value (PPV), and lower feed conversion ratio (FCR) compared to other groups. Dietary protein and lipid levels had significantly (P < 0.05) affected whole-body composition. Second-order polynomial regression analysis showed that the optimal dietary protein and lipid requirements for climbing perch fry are in the ranges of 40.54—40.68% and 8.17—8.30%, respectively. These findings provide valuable insights for formulating nutritionally balanced diets to support optimal growth and development of A. testudineus fry. [ABSTRACT FROM AUTHOR]

Abstract Soil salinization, a prevalent form of environmental stress, leads to significant soil desertification and impacts agricultural productivity by altering the internal soil environment, slowing cellular metabolism, and modifying cellular architecture. This results in a marked reduction in both the yield and diversity of crops. Maize, which is particularly susceptible to salt stress, serves as a critical model for studying these effects, making the elucidation of its molecular responses essential for crop improvement strategies. This study focuses on the phytochrome-interacting factor 3 (PIF3), previously known for its role in freezing tolerance, to assess its function in salt stress tolerance. Utilizing two transcript variants of maize ZmPIF3 (ZmPIF3.1 and ZmPIF3.2), we engineered Arabidopsis transgenic lines to overexpress these variants and analyzed their phenotypic, physiological, biochemical, and transcriptomic responses to salt stress. Our findings reveal that these transgenic lines displayed not only enhanced salt tolerance but also improved peroxide decomposition and reduced cellular membrane damage. Transcriptome analysis indicated significant roles of hormonal and Ca2+ signaling pathways, along with key transcription factors, in mediating the enhanced salt stress response. This research underscores a novel role for ZmPIF3 in plant salt stress tolerance, offering potential avenues for breeding salt-resistant crop varieties.

Abstract [Objective] The study aims to examine the characteristics of innate immune physiological responses induced by chitin and flagellin-derived peptide flg22 in pepper seedlings, and to explore the relationship between innate immune physiological responses and pepper multiple resistance to diseases. [Methods] Five local pepper cultivars from Sichuan were used as experimental materials. The disease indexes of bacterial wilt and phytophthora blight in pepper seedlings were analyzed. Seedlings were cultured by hydroponics and treated with chitin and flg22 exogenously. Root growth, stomata aperture, callose deposition, reactive oxygen species (ROS) accumulation, SOD, CAT activities as well as gene expression under different induction times were analyzed. The physiological responses and their relationship with disease resistance were evaluated comprehensively using biostatistics. [Results] (1) The lowest disease index of bacterial wilt and phytophthora blight was ‘Chuanteng No.10’ (‘CT10’) cultivar, which was the strongest in disease resistance. The highest disease index of bacterial wilt and phytophthora blight was ‘Tiaojiao’ (‘TJ’) cultivar, which was the weakest in disease resistance. (2) Exogenous chitin and flg22 inhibited the root growth rate of pepper seedlings in five cultivars, induced stomatal closure on detached leaves, promoted callose deposition in cell wall, and increased ROS levels with improved SOD and CAT activities. By calculating the average membership function value of the innate immune physiological response, it showed that the highest was ‘CT10’ and the lowest was ‘TJ’. The average membership function value has a significant negative correlation with the disease index of bacterial wilt and phytophthora blight. (3) flg22 and chitin induced the expression of genes involved in innate immune response in ‘CT10’, such as CaWRKY22, CaMAPK7, and ChiIV3. [Conclusion] Exogenous flg22 and chitin induce the innate immune physiological responses in pepper seedlings, with variations among the varieties. The average membership function of ‘CT10’ is the highest and the multi-resistance level is the best, due to the up-regulated expression of CaWRKY22, CaMAPK7, and ChiIV3.

Long-term storage of crop seeds is critical for the conservation of germplasm resources, ensuring food supply, and supporting sustainable production. Rice, as a major food staple, has a substantial stock for consumption and production worldwide. However, its food value and seed viability tend to decline during storage. Understanding the physiological responses and molecular mechanisms of aging tolerance forms the basis for enhancing seed storability in rice. This review outlines the latest progress in influential factors, evaluation methods, and identification indices of seed storability. It also discusses the physiological consequences, molecular mechanisms, and strategies for breeding aging-tolerant rice in detail. Finally, it highlights challenges in seed storability research that require future attention. This review offers a theoretical foundation and research direction for uncovering the mechanisms behind seed storability and breeding aging-tolerant rice.

Establishment of oak seedlings, which is an important factor in forest restoration, is affected by drought that hampers the survival, growth, and development of seedlings. Therefore, it is necessary to understand how seedlings respond to and recover from water-shortage stress. We subjected seedlings of two oak species, Quercus acutissima and Quercus palustris, to drought stress for one month and then rewatered them for six days to observe physiological and genetic expression changes. Phenotypically, the growth of Q. acutissima was reduced and severe wilting and recovery failure were observed in Q. palustris after an increase in plant temperature. The two species differed in several physiological parameters during drought stress and recovery. Although the photosynthesis-related indicators did not change in Q. acutissima, they were decreased in Q. palustris. Moreover, during drought, content of soluble sugars was significantly increased in both species, but it recovered to original levels only in Q. acutissima. Malondialdehyde content increased in both the species during drought, but it did not recover in Q. palustris after rewatering. Among the antioxidant enzymes, only superoxide dismutase activity increased in Q. acutissima during drought, whereas activities of ascorbate peroxidase, catalase, and glutathione reductase increased in Q. palustris. Abscisic acid levels were increased and then maintained in Q. acutissima, but recovered to previous levels after rewatering in Q. palustris. RNA samples from the control, drought, recovery day 1, and recovery day 6 treatment groups were compared using transcriptome analysis. Q. acutissima exhibited 832 and 1076 differentially expressed genes (DEGs) related to drought response and recovery, respectively, whereas Q. palustris exhibited 3947 and 1587 DEGs, respectively under these conditions. Gene ontology enrichment of DEGs revealed "response to water," "apoplast," and "Protein self-association" to be common to both the species. However, in the heatmap analysis of genes related to sucrose and starch synthesis, glycolysis, antioxidants, and hormones, the two species exhibited very different transcriptome responses. Nevertheless, the levels of most DEGs returned to their pre-drought levels after rewatering. These results provide a basic foundation for understanding the physiological and genetic expression responses of oak seedlings to drought stress and recovery. [ABSTRACT FROM AUTHOR]

Cultivation of sugar beet (Beta vulgaris L. subsp. vulgaris) is vital for sugar and industrial uses, but it faces challenges like drought and cold in northern regions of China. Enhancing stress tolerance, especially in seedlings, is crucial. The primary objective of this study was to investigate the influence of TM35 (mepiquat chloride (MC) production waste) on sugar beet seedlings. We added varying concentrations of TM35 (0, 0.5, 1, 2, and 4% relative to soil dry weight) into the soil. After 20 days of cultivation, we assessed the growth, physiological indicators, and nutrient levels of sugar beet seedlings, followed by a second round of measurements after subjecting the sugar beets to drought and freezing stress. Under normal conditions, TM35 reduced sugar beet height, leaf area, and osmoregulatory substances (soluble protein, soluble sugar, proline), but boosted biomass, leaf number, photosynthesis (Pn), and chlorophyll. Under drought or freezing stress, TM35 had the same effects on growth as under normal conditions. It also reduced electrolyte leakage (EL) and malondialdehyde (MDA) levels in sugar beet leaves, increased osmoregulatory substances (soluble proteins, soluble sugars, proline), and decreased antioxidant enzyme activities (peroxidase, POD; superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX). Notably, TM35 increased the survival of sugar beet seedlings under freezing stress. Based on the growth of sugar beet seedlings under normal conditions and the combined growth and physiological responses of sugar beet under drought and freezing stress, applying 0.5% TM35 in soil under normal conditions can promote the growth of sugar beet, while applying 2% TM35 under drought or freezing stress can improve the resistance of sugar beet to stress. [ABSTRACT FROM AUTHOR]

Global warming has profound effects on the living conditions and metabolism of organisms, including fish. The metabolic rate of fish increases as the temperature increases within its thermal tolerance range. Temperature changes can trigger a range of physiological reactions, including the activation of the stress axis and the production of HSPs. Under stress conditions, HSPs play a crucial role in antioxidant systems, immune responses, and enzyme activation. This study examined the effects of heat shock products (HSPs) on fish under temperature stress. Various HSP inducers (HSPis), including Pro-Tex®, amygdalin, and novel synthetic compounds derived from pirano piranazole (SZ, MZ, HN-P1, and HN-P2), were evaluated in isolated cells of sterlet sturgeon (Acipenser ruthenus) treated with temperature changes (18, 22, and 26 °C). Cells from the liver, kidney, and gills were cultured in vitro in the presence and absence of temperature stress and treated with HSPi compounds. To assess HSP27, HSP70, and HSP90 expression patterns, Western blotting was used. The HSPis and HSPi + temperature stress treatments affected the antioxidant capacity and immune parameters, among other enzyme activities. The results showed that HSPi compounds increase cell survival in vitro, positively modulate HSP expression and antioxidant levels, and decrease immune parameters. HSPi can increase A. ruthenus tolerance to temperature stress. In addition, the results indicate that these compounds can reverse adverse temperature effects. Further research is needed to determine how these ecological factors affect fish species' health in vivo and in combination with other stressors. [ABSTRACT FROM AUTHOR]

Hylurgus ligniperda invaded Shandong, China, through imported forest timber, posing a threat to China's forest health. Exotic insects with broad environmental tolerance, including low temperatures, may have a better chance of surviving the winters and becoming invasive. Understanding the cold-tolerance strategies of H. ligniperda may help to design sustainable pest management approaches. In this study, we aim to investigate the cold-tolerance ability and relevant physiological indicators in overwintering H. ligniperda adults to determine any possible overwintering strategies. Supercooling points (SCPs) for adults H. ligniperda differed significantly across months and reached the lowest level in the mid- and post-overwintering period, the minimum SCPs −6.45 ± 0.18 °C. As the cold exposure temperature decreased, the survival rate of adults gradually decreased, and no adult survived more than 1 day at −15 °C, and the LLT50 for 1 day was −7.1 °C. Since H. ligniperda adults can survive internal ice formation, they are freeze-tolerant insects. Throughout the overwintering period, the SCPs and the water, protein, sorbitol, and glycerol content in adults decreased initially and then increased. We reported significant correlations between total protein, sorbitol, trehalose, and glycerol content in the beetles and SCPs. Glycogen, lipid, protein, trehalose, and sorbitol content in adult beetles may directly affect their cold-tolerance capacity and survival during winter. This study provides a physiological and biochemical basis for further study of metabolism and cold-tolerance strategies in H. ligniperda adults, which may help predict population dynamics and distribution potential of pests. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the biochemical indices in the saliva of leisure and sport horses undergoing different levels of workload. The experiment was conducted on 40 horses (4--28 years): sport horses (n = 20), and leisure horses (n = 20). The saliva samples were collected non-invasively 15 min before riding (A), 1 min before riding (B), 1 min after riding (C) and 15 min after riding (D). Albumin (Alb), alkaline phosphatase (ALP), α-amylase (AMY), creatine kinase (CK), creatinine (Crea), glucose (Glc), lactate (Lac), triacylglycerols (TG), total protein (TP), urea and lipid peroxidation (TBARS) were measured in saliva. Riding caused a significant change in many biochemical indices (Alb, CK, Glc, Lac, TBARS and urea) compared to baseline values in leisure horses (P < 0.05) but only in a few indices (Glc, Crea and TBARS) in sport horses. On the other hand, when comparing groups of leisure and sport horses, higher concentrations of Alb, Glc, TG and urea were found in sport horses whereas raised concentrations of CK and Crea were found in leisure horses. The obtained results indicate the adaptation of trained horses to physical effort. Moreover, this study confirms other possibilities of using salivary biochemical properties for physical stress assessment. Other more detailed comparative studies of load response in horses may provide useful information to quantify the reference range of individual stress indices. [ABSTRACT FROM AUTHOR]

Plants play a crucial role as a removal pathway in constructed wetlands, demonstrating the ability to absorb and tolerate antibiotics from wastewater. However, the specific contribution of plants in this regard has not yet to be sufficiently established. To gain a more comprehensive insight into the associated processes, we selected three common wetland plant species, Canna indica L. (C. indica), Cyperus alternifolius L. (C. alternifolius), and Thalia dealbata Fraser (T. dealbata), to evaluate their capacity for uptake, accumulation, and physiological response in the removal of sulfamethoxazole (SMX) at varying initial concentrations (10, 30, 100, and 300 µg/L) under hydroponic conditions. The results showed that SMX removal was more efficient at lower concentrations (10 and 30 µg/L) than at higher concentrations (100 and 300 µg/L). Moreover, plant systems were found to consistently outperform unplanted systems in SMX removal. Among the assessed species, C. indica was identified as being relatively effective in the removal of SMX, whereas the performance of C. alternifolius was notably less pronounced. A positive correlation was observed between the concentration of SMX in the plant tissues and that in the external aqueous medium. However, plant tissue residues contributed only a minor fraction to the overall removal of SMX. Wetland plants absorb SMX through their roots, and we accordingly detected significantly higher concentrations in submerged plant tissues. Furthermore, we also detected reductions in net photosynthetic rates indicative of potential phytotoxicity, which is associated with the accumulation of antibiotic in the shoot tissues. Accumulation of SMX in the roots and rhizomes was also found to be associated with the development of shorter roots, with this effect becoming more pronounced with an increase in the concentration of exogenous SMX. However, despite these adverse effects, plants can detoxify antibiotics via the glutathione pathway. Of the assessed plant species, C. indica was identified as the most SMX tolerant, as indicated by Km and Vmax values, with C. alternifolius being the least tolerant. Our findings in this study reveal the potential value of wetland plants in the sequestration of antibiotics and provide evidence for the underlying mechanisms of action. These findings could make an important contribution to the implementation of phytoremediation in antibiotic-contaminated water. Three wetland plants with fibrous root systems, namely Canna indica, Cyperus alterniflius, and Thalia dealbata, were selected to investigate the removal efficiencies of sulfamethoxazole in the hydroponic system by different emergent plants, quantify the contribution of uptake and accumulation for sulfamethoxazole in plant tissues, and assess the physiological responses of plants and their effect on the removal of sulfamethoxazole. The knowledge obtained from this study shows the potential use of wetland plants for removing antibiotics and the inherent mechanisms, which will be useful for the application of phytoremediation in antibiotic contaminated water. [ABSTRACT FROM AUTHOR]

We recruited 162 healthy elderly adults to determine the thermal, physiological, and psychological effects of horticultural activities (flower arranging, transplanting, and rubble masonry) in outdoor open spaces. We linked these to local climate conditions, physiology, and comfort through a questionnaire survey. The results showed that: (1) the neutral physiological equivalent temperature (NPET) before the horticultural activities were 22.18 ℃ for flower arranging, 23.67 ℃ for transplanting, and 20.78 ℃ for rubble masonry, while the NPET decreased to 18.53 ℃, 20.73 ℃ and 18.04 ℃ (respectively) after activities. (2) The heart rate and blood oxygen saturation changed significantly (p < 0.05) only after rubble masonry. (3) The average positive affect (PA) scores increased after flower arranging by 4.83, transplanting by 3.30, and rubble masonry by 4.00. (4) After activities, the thermal sensation vote was mainly influenced by globe temperature (41.36%), air temperature (33.47%), and wind speed (25.17%). Thermal comfort vote could be promoted because of 37.35% of an increasing positive and 21.20% of decreasing negative emotion. [ABSTRACT FROM AUTHOR]

Long-term storage of crop seeds is critical for the conservation of germplasm resources, ensuring food supply, and supporting sustainable production. Rice, as a major food staple, has a substantial stock for consumption and production worldwide. However, its food value and seed viability tend to decline during storage. Understanding the physiological responses and molecular mechanisms of aging tolerance forms the basis for enhancing seed storability in rice. This review outlines the latest progress in influential factors, evaluation methods, and identification indices of seed storability. It also discusses the physiological consequences, molecular mechanisms, and strategies for breeding aging-tolerant rice in detail. Finally, it highlights challenges in seed storability research that require future attention. This review offers a theoretical foundation and research direction for uncovering the mechanisms behind seed storability and breeding aging-tolerant rice. [ABSTRACT FROM AUTHOR]

The phytoremediation of soil and water that has been significantly contaminated with metals has potential ecological and economical ramifications, as well as the advantages of high efficiency, and is an environmentally friendly method of ecological pollution control. This study aimed to examine the impact of varying concentrations of Copper (Cu2+) (0, 5, 10, 15, 20, and 25 mg·L−1) on the growth, development, physiology, biochemistry, mineral elements, and features of Cu2+ enrichment of Impatiens uliginosa. This plant is endemic to Yunnan Province in China and is a wetland species. The results showed that the root lengths, stem diameters, plant height, and stem and leaf biomass of I. uliginosa showed a phenomenon of "low promotion and high inhibition," while the root biomass showed a trend of gradual decreasing. At the early stage of Cu2+ stress (day 6), the activities of peroxidase and catalase and the contents of malondialdehyde (MDA) of I. uliginosa were directly proportional to the concentration of Cu2+. As the treatment time increased, the activation of a defense mechanism in vivo enabled I. uliginosa to adapt to the high Cu2+ environment, and the content of MDA gradually decreased. As the concentration of Cu2+ increased, its contents in the roots, stems, and leaves also gradually increased. In particular, when the concentration of Cu2+ reached 25 mg·L−1, its contents in the roots of I. uliginosa increased by 39.16-fold compared with that of the control group (CK). The concentration-dependent influence of the contents of iron (Fe) and zinc (Zn) in the roots and leaves were observed. Low concentrations of Cu2+ promoted iron content in roots and leaves, and vice versa, while Zn content decreased with the increasing concentration of Cu2+. It was conclusively shown that I. uliginosa has the potential to remediate low concentrations of Cu2+ pollution in water and is a textbook ornamental plant to remediate bodies of water that are polluted with Cu2+. [ABSTRACT FROM AUTHOR]

A Living wall system (LWS) is a biophilic design element that has been introduced into indoor environments in recent years. Previous studies have demonstrated that the LWS improves indoor visual comfort and air quality. However, studies on its psychological effects on occupants are still scarce. In this paper, the psychological effects were investigated by recording and analyzing the parameters of occupants' physiological responses including an electroencephalogram (EEG) and heart rate variability (HRV). A cross-over experiment was conducted among 43 participants under three different desk work environments based on various materials involving an LWS, a fake LWS, and a bare white wall. The results conclude that LWSs effectively reduce the accumulation speed of negative psychological states caused by desk work to about 1/3, compared with a regular office environment without an LWS. However, occupants tend to be less excited and focused after resting in the LWS environment. Therefore, it is recommended to place LWSs in the rest area rather than close to the desk area in an office building, considering the balance between mental health and work efficiency. [ABSTRACT FROM AUTHOR]

UV-B is an important environmental factor that differentially affects plant growth and secondary metabolites. The effects of supplemental ultraviolet-B (sUV-B) exposure (T1, 1.40 kJ·m−2·day−1; T2, 2.81 kJ·m−2·day−1; and T3, 5.62 kJ·m−2·day−1) on the growth biomass, physiological characteristics, and secondary metabolites were studied. Our results indicated that leaf thickness was significantly (p < 0.05) reduced under T3 relative to the control (natural light exposure, CK); The contents of 6-BA and IAA were significantly reduced (p < 0.05); and the contents of ABA, 10-deacetylbaccatin III, and baccatin III were significantly (p < 0.05) increased under T1 and T2. The paclitaxel content was the highest (0.036 ± 0.0018 mg·g−1) under T3. The cephalomannine content was significantly increased under T1. Hmgr gene expression was upregulated under T1 and T3. The gene expressions of Bapt and Dbtnbt were significantly (p < 0.05) upregulated under sUV-B exposure, and the gene expressions of CoA, Ts, and Dbat were significantly (p < 0.05) downregulated. A correlation analysis showed that the 6-BA content had a significantly (p < 0.05) positive correlation with Dbat gene expression. The IAA content had a significantly (p < 0.05) positive correlation with the gene expression of Hmgr, CoA, Ts, and Dbtnbt. The ABA content had a significantly (p < 0.05) positive correlation with Bapt gene expression. Dbat gene expression had a significantly (p < 0.05) positive correlation with the 10-deacetylbaccatin content. Hmgr gene expression was positively correlated with the contents of baccatin III and cephalomannine. Bapt gene expression had a significantly (p < 0.01) positive correlation with the paclitaxel content. A factor analysis showed that the accumulation of paclitaxel content was promoted under T2, which was helpful in clarifying the accumulation of taxane compounds after sUV-B exposure. [ABSTRACT FROM AUTHOR]

Flooding stress is an increasingly serious problem in wetlands, often affecting large areas of crops and timber production areas. The current study aimed to explore the species differences in responses to flooding stress between Q. nuttallii and Q. palustris in an outdoor environment. All the tested plants survived after a 60-day flooding treatment that left 5 cm of water above the soil surface. This suggests that the two species are flood-tolerant, so they can be applied in the construction of riparian protection forests and wetland restoration. Compared with control conditions, flooding treatment significantly decreased seedling height and diameter and the Pn, Gs, Tr, Fv/Fm, ABS/CSm, TR0/CSm, ET0/CSm, RE0/CSm, IAA, and GA3 content and significantly increased the content of MDA, H2O2, soluble sugars, SOD, POD, ADH, ABA, and JA. Under control conditions, Q. nuttallii showed significantly greater growth and photosynthetic capability than Q. palustris. In contrast, Q. palustris exhibited less inhibition of growth and photosynthesis, oxidative stress levels, and antioxidant enzyme activities than Q. nuttallii under flooding conditions. The findings indicate that Q. palustris has better defense mechanisms against the damage caused by flooding stress than Q. nuttallii. Q. nuttallii was more sensitive and responsive to flooding than Q. palustris. [ABSTRACT FROM AUTHOR]

Purpose: The aim was to analyze the differences between wheelchair basketball players with Spinal Cord Injury (SCI) and without Spinal Cord injury (Non-SCI) in psycho-physiological response and neuromuscular fatigue in a series of small-sided games (SSG). Methods: Thirteen high-level wheelchair basketball male players (age 28.8 ± 9.2 years, body mass 75.9 ± 11.3 kg) participated in four versus four SSG once a week for five consecutive weeks. Absolute and relative heart rate (HR) during SSG and muscular and respiratory perceived exertion (RPEmus and RPEres), tympanic temperature, and acceleration capacity were recorded at the end of each SSG. Results: No significant differences were observed between SCI and non-SCI players in relative HR variables in SSG series. However, the SCI group had a lower absolute HR (mean HR and peak HR) (p <.05 or p <.01, ES = 0.73–1.39) and higher perceived muscular load (RPEmusTL) (p <.01, ES = 0.75–0.82) during SSG. Regarding the evolution of the physiological response, no differences were observed between the four bouts in the SCI group. However, the Non-SCI group showed an increase in the absolute and relative variables of HR and RPEres and RPEmus (p <.05 or p <.01, ES = 0.45–1.06). On the contrary, only the SCI group showed a loss of acceleration capacity between the four series (p <.05, ES = 0.28–0.35). Conclusion: The results of this study show that there are differences between SCI and non-SCI wheelchair basketball players in their physiological response and acceleration capacity during SSG tasks. [ABSTRACT FROM AUTHOR]

Aeollanthus repens, native to East Africa, thrives in seasonally dry tropical biomes and boasts qualities ideal for both ornamental and ground cover purposes. However, despite its potential, its current resistance levels remain uncertain. Assessing its adaptability could offer valuable insights for its wider adoption and utilization. In this study, researchers employed 3-month-old cuttings of A. repens, subjecting them to six distinct environments by manipulating the temperature (25/20 °C and 35/30 °C) and soil moisture levels (100%, 20%, and 40%). Their leaf physiological and photosynthetic indices were assessed at intervals of 5, 10, and 15 days following exposure to stress. The findings unveiled that exposure to prolonged moisture, elevated temperatures, or a combination of both led to an increase in osmoregulatory substances in the leaves. This increase was accompanied by heightened enzyme activity and an increased intercellular carbon dioxide concentration, followed by a subsequent decline. Additionally, chlorophyll content, net photosynthetic rate, stomatal conductance, and transpiration rate exhibited a decreasing trend over time. Through a comprehensive assessment of stress tolerance utilizing a composite affiliation function value index, the study concluded that A. repens exhibits optimal growth in a certain high-temperature environments and demonstrates substantial resistance to waterlogging, drought, and simultaneous high-temperature stress. However, the resilience of A. repens appears to diminish under the compounded stresses of high temperature and drought. [ABSTRACT FROM AUTHOR]

Hypoxia is a globally pressing environmental problem in aquatic ecosystems. In the present study, a comprehensive analysis was performed to evaluate the effects of hypoxia on physiological responses (hematology, cortisol, biochemistry, hif gene expression and the HIF pathway) of hybrid sturgeons (Acipenser schrenckii ♂ × Acipenser baerii ♀). A total of 180 hybrid sturgeon adults were exposed to dissolved oxygen (DO) levels of 7.00 ± 0.2 mg/L (control, N), 3.5 ± 0.2 mg/L (moderate hypoxia, MH) or 1.00 ± 0.1 mg/L (severe hypoxia, SH) and were sampled at 1 h, 6 h and 24 h after hypoxia. The results showed that the red blood cell (RBC) counts and the hemoglobin (HGB) concentration were significantly increased 6 h and 24 h after hypoxia in the SH group. The serum cortisol concentrations gradually increased with the decrease in the DO levels. Moreover, several serum biochemical parameters (AST, AKP, HBDB, LDH, GLU, TP and T-Bil) were significantly altered at 24 h in the SH group. The HIFs are transcription activators that function as master regulators in hypoxia. In this study, a complete set of six hif genes were identified and characterized in hybrid sturgeon for the first time. After hypoxia, five out of six sturgeon hif genes were significantly differentially expressed in gills, especially hif-1α and hif-3α, with more than 20-fold changes, suggesting their important roles in adaptation to hypoxia in hybrid sturgeon. A meta-analysis indicated that the HIF pathway, a major pathway for adaptation to hypoxic environments, was activated in the liver of the hybrid sturgeon 24 h after the hypoxia challenge. Our study demonstrated that hypoxia, particularly severe hypoxia (1.00 ± 0.1 mg/L), could cause considerable stress for the hybrid sturgeon. These results shed light on their adaptive mechanisms and potential biomarkers for hypoxia tolerance, aiding in aquaculture and conservation efforts. [ABSTRACT FROM AUTHOR]

Soil is home to microbiota with diverse metabolic activities. These microorganisms play vital roles in many ecological processes. Thus, the assessment of microbial functional diversity is an important quality indicator of soil ecosystems. In this study, we collected soil samples from three distinct forest habitats, i.e., an agroforest, a primary forest (PF), and a secondary forest, within the Angat Watershed Reservation in Bulacan, Northern Philippines. Community-level physiological profiling (CLPP) was done with the BIOLOG EcoPlate™ to analyze the responses of the soil microbial communities from the three forest habitats in the absence or presence of antibiotics. The BIOLOG EcoPlate represents 31 utilizable carbon sources. Based on the CLPP analysis, soil samples from the PF showed significantly higher utilization of most carbon sources than the other forest types (p < 0.05). Thus, less disturbed forest types constitute more functionally diverse microbial communities. The presence of antibiotics significantly decreased the carbon utilization patterns of the soil microbial communities (p < 0.05), indicating the possible use of CLPP in monitoring contamination in soil. [ABSTRACT FROM AUTHOR]

Selenium (Se) is crucial for both plants and humans, with plants acting as the main source for human Se intake. In plants, moderate Se enhances growth and increases stress resistance, whereas excessive Se leads to toxicity. The physiological mechanisms by which Se influences rice seedlings' growth are poorly understood and require additional research. In order to study the effects of selenium stress on rice seedlings, plant phenotype analysis, root scanning, metal ion content determination, physiological response index determination, hormone level determination, quantitative PCR (qPCR), and other methods were used. Our findings indicated that sodium selenite had dual effects on rice seedling growth under hydroponic conditions. At low concentrations, Se treatment promotes rice seedling growth by enhancing biomass, root length, and antioxidant capacity. Conversely, high concentrations of sodium selenite impair and damage rice, as evidenced by leaf yellowing, reduced chlorophyll content, decreased biomass, and stunted growth. Elevated Se levels also significantly affect antioxidase activities and the levels of proline, malondialdehyde, metal ions, and various phytohormones and selenium metabolism, ion transport, and antioxidant genes in rice. The adverse effects of high Se concentrations may directly disrupt protein synthesis or indirectly induce oxidative stress by altering the absorption and synthesis of other compounds. This study aims to elucidate the physiological responses of rice to Se toxicity stress and lay the groundwork for the development of Se-enriched rice varieties. [ABSTRACT FROM AUTHOR]

Adaptability and significant economic value of the neem tree are well-known, as it can flourish in a variety of environmental conditions. While the neem seed production is highly sensitive to prevailing weather conditions during the reproductive phase and flowering behaviour of the tree. A study was conducted at Tamil Nadu Agricultural University, Coimbatore in 2023 with the primary objective of validating the weather influence on neem seed production using the logics of physiological responses, as a continuation of research conducted the previous year (2022). During the pre-flowering and flowering stages, diverse weather conditions led to notable changes in the physiological traits of neem trees, which displayed varying patterns of flowering. Trees that flowered consistently showed elevated levels of indole acetic acid (IAA) oxidase, relative water content, and nitrate reductase compared to those that lacked flowers or produced intermittently. In the flowering stage, the neem trees responded positively in terms of physiological aspects like IAA oxidase, relative water content, nitrate reductase, and exhibited lower proline levels, which can be attributed to the optimal maximum temperature, rainfall, and soil moisture. Proline levels rose during the pre-flowering stage due to soil moisture deficits but fell during the flowering stage with the onset of rain. These physiological changes, driven by climatic factors, are likely to enhance the flowering, fruiting, and overall yield of neem trees. [ABSTRACT FROM AUTHOR]

The use of personal protective equipment (PPE) for virus handling has the side effect of heat stress, which requires intervention to improve. This study aimed to evaluate the comfort of a newly designed PPE ensemble for virus handling. Three types of PPE ensembles were tested: reg-ular PPE as a control, PPE plus breathable cooling wear (cooling wear), and PPE plus a portable airflow cooling device (cooling device). Twelve participants simulated six activities, including physical activities, activities requiring concentration, and manual dexterity activities, for one hour. The microclimate conditions, perceived discomfort, and physiological responses were measured after each experimental activity. The results show that the use of cooling wear and a cooling device had a significant effect on the microclimate conditions, perceived comfort, and physiological responses of users, proving superior to the use of regular PPE. A cooling device can improve the microclimate more than cooling wear, thereby directly increasing perceived comfort and decreasing physiological responses. It can be concluded that the use of cooling wear and a cooling device effectively increases the comfort of wearing PPE. The cooling device is more suitable for use in tropical climates with hot and humid characteristics, so it is a better choice than cooling wear. [ABSTRACT FROM AUTHOR]