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Animal waste can contribute substantially to nutrient cycling and ecosystem productivity in many environments. However, little is known of the biogeochemical impact of animal excretion in wetland habitats. Here we investigate the effects of wood frog (Lithobates sylvaticus) tadpole aggregations on nutrient recycling, microbial metabolism and carbon cycling in geographically isolated wetlands. We used a paired mesocosm and field study approach that utilized measurements of tadpole excretion rates, microbial extracellular enzyme activities, and litter degradation. We found a strong relationship between tadpole development and nutrient excretion, demonstrating that ontological changes impact tadpole‐mediated nutrient cycling in wetland habitats. Further, the interplay between population‐level tadpole excretion and wetland hydrologic conditions increased ambient NH4+$$ {\mathrm{NH}}_4^{+} $$ and PO43−$$ {\mathrm{PO}}_4^{3-} $$ concentrations by 56 and 14 times, respectively, compared to adjacent wetlands without tadpoles. Within our mesocosm study, microbes decreased extracellular enzyme production associated with nitrogen acquisition in response to the presence of tadpole‐derived nitrogen. In addition to microbial metabolic responses, tadpole presence enhanced litter breakdown in both mesocosms and wetlands by 7% and 12%, respectively, in comparison to reference conditions. These results provide evidence for the functional and biogeochemical role of tadpole aggregations in wetland habitats, with important implications for ecosystem processes, biodiversity conservation, and ecosystem management. [ABSTRACT FROM AUTHOR]

Background: Despite the widespread use in ischemic stroke, cancer, and malnutrition, the predictive ability of serum albumin to globulin ratio (A/G) among patients suffering from aneurysmal subarachnoid hemorrhage (aSAH) remains unknown. This study aimed to determine if serum A/G ratio is associated with the occurrence of delayed cerebral ischemia (DCI) after aSAH. Methods: We retrospectively viewed the medical records of aSAH patients from 08/2017 to 08/2022. Serum albumin and globulin laboratory test results were collected within 24 hours after admission. Serum A/G were dichotomized based on whether the DCI occurred. Logistic regression was used to determine the predictors of DCI. The relationship between serum A/G and the occurrence of DCI was analyzed with receiver operating characteristic(ROC) curve. Results: A total of 363 eligible patients with aSAH were included in the study, among which DCI occurred in 87 patients(23.97%). Serum A/G[OR=2.720, 95%CI (1.190-6.270), P=0.018], non-surgical[OR=0.228, 95%CI (0.065-0.621), P=0.008], lactate dehydrogenase[OR=1.004, 95%CI (1.000-1.008), P=0.029], P[OR=0.354, 95%CI (0.130-0.926), P=0.038], plasma fibrinogen[OR=1.266, 95%CI (1.019-1.583), P=0.035] were associated with the occurrence of DCI. ROC showed that serum A/G, non-surgical, LDH, P, plasma fibrinogen could predict the occurrence of DCI in aSAH patients with values 0.575, 0.560, 0.602, 0.571 and 0.539 for serum A/G, non-surgical, LDH, P, plasma fibrinogen, respectively. Conclusions: In conclusion, serum A/G levels are correlated with DCI in individuals with aSAH, and high serum A/G levels on admission may be associated with the occurrence of DCI. [ABSTRACT FROM AUTHOR]

Corn grain with a high phosphorus (P) content (mainly in the form of phytate-P) may need to be processed to improve the digestibility of nutrients for young calves. Processing corn grains can improve the accessibility of phytate-P to the rumen enzymes and increase the bioavailability of P, which benefits the growth and development of calves. The objective of this study was to investigate the effects of feeding starter diets with steam-flaked corn (SFC) compared with ground corn (GC) with 2 P contents of 0.4% and 0.7% DM basis on intake, growth performance, nutrient digestibility, blood metabolites and urinary purine derivatives (PD) in dairy calves. A total of 48 female Holstein dairy calves (3 d old; average initial weight 39.7 ± 3.9 kg) were randomly assigned to a 2 × 2 factorial arrangement of treatments (12 calves/treatment) in a randomized complete block design. The treatment groups were: (1) a starter diet of GC with 0.4% P (GC-0.4P); (2) a starter diet of GC with 0.7% P (GC-0.7P); (3) a starter diet of SFC with 0.4% P (SFC-0.4P); (4) a starter diet of SFC with 0.7% P (SFC-0.7P). Calves received 6 L/d of transition milk on d 2 to 3 and 5 L/d of whole milk on d 4 to 30, which was increased to 7 L/d on d 31 to 45, then decreased to 5 L/d on d 46 to 60 and reduced to a single feeding of 2 L on d 61 to 62. All calves had free access to starter feed and water. All calves were weaned on d 63 and remained in the study until d 83. Rumen fluid samples were collected on d 38 (preweaning) and d 76 (postweaning). Blood samples were collected on d 40 and 80 and urine samples were collected on 4 consecutive days from d 79 to 82 to analyze urinary excretion of PD. The phytate-P content ranged from 0.23 to 0.17 for GC and SFC, respectively. In particular, the interaction between corn processing method and P content showed that the SFC-0.7P diets had a greater intake of starter feed during the pre- and postweaning periods compared with the other experimental groups. In addition, calves fed the SFC-0.7P diet had greater ADG, BW, withers height at weaning, better OM digestibility, higher blood BHB levels, and higher microbial protein synthesis compared with all other groups. Feeding the SFC diet also resulted in improved feed efficiency, improved P digestibility, and a tendency toward a lower rumen pH, albeit with a tendency toward an increase in blood glucose concentration during the preweaning period. In addition, the inclusion of 0.7% P to the starter diet resulted in increased fiber digestibility and a slight improvement in growth performance, which was particularly evident in hip height. Overall, the inclusion of SFC in the calf starter diet, especially in combination with a 0.7% DM basis P supplement, improved growth performance and nutrient utilization in dairy calves compared with GC. The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. [ABSTRACT FROM AUTHOR]

Accurate real-time estimation of nutrient concentrations in potato (Solanum tuberosum L.) canopies is crucial for advanced decision support systems in site-specific nutrient management. This study investigated the effectiveness of unmanned aerial vehicle (UAV) based hyperspectral imaging in predicting nitrogen (N), phosphorus (P), potassium (K), and sulfur (S) concentrations in potato plants comparing two sampling types such as petiole/leaves and above-ground biomass (AGB) sampling. Furthermore, this study also investigates the prediction of AGB, total, and marketable yield of two potato cultivars, 'Atlantic' (chipping) and 'Red La Soda' (tablestock). Four UAV flights over experimental sites were made, and hyperspectral imaging sensors (393–995 nm, 273 bands) were conducted, which coincided with the in-field sample collection as ground truth. Data were analyzed using the partial least square regression model after preprocessing and extracting spectra from images. The model showed high accuracy in estimating plant N concentration from petiole/leaf samples (external validation R2 = 0.58; [external validation RMSE = 0.31 × 104 mg kg−1]), as well as for P (0.75 [0.05 × 104 mg kg−1]) and S (0.58 [0.03 × 104 mg kg−1]). Potassium estimation accuracy improved with biomass sampling (0.47 [1.19 × 104 mg kg−1]). Above-ground biomass estimation had higher accuracy for 'Atlantic' (0.75 [1.29 Mg ha−1]) than for 'Red La Soda' (0.57 [1.38 Mg ha−1]). The model accurately estimated total and marketable tuber yields for both cultivars, with variations noted based on flight timing related to the crop stage. Cultivar 'Red La Soda' achieved the highest total yield accuracy on the first (0.76 [3.31 Mg ha−1]) and fourth flights (0.76 [3.31]), while the 'Atlantic' had the highest accuracy on the third flight (0.50 [4.11]). Model outputs, including standardized coefficients and variable importance in prediction, visualizing band contributions to measured parameter predictions are presented. This study concludes that hyperspectral imaging successfully estimates the potato nutrient concentration and predicts the in-season potato yield, which can contribute significantly to the potato management decision support system. However, it underscores the importance of multiyear high temporal data acquisition with variable potato varieties to establish a reliable AGB and yield estimation model to improve performance. [ABSTRACT FROM AUTHOR]

Nutritive components such as crude protein, crude fibre, cellulose, hemicellulose, lignin, nitrogen, phosphorus and potassium, of important fodder plant species in successional grassland in Manipur have been evaluated throughout the year. The amount of crude protein and fibre varied from 2.38% (January) to 25.75% (May) and 25.20% (January) to 69.87% (July), respectively in different species. Cellulose, hemicellulose and lignin contents also ranged from 37.19% (March) to 60.42% (May), 19.89% (March) to 44.59% (September) and 4.28% (March) to 28.21% (January), respectively, in different species across the months. The highest nutritive value of plant species i.e., nitrogen, phosphorus and potassium content was found to be during the rainy months of July/September. Out of 13 fodder plant species, Desmodium triflorum exhibited highest content for crude protein and nitrogen, Fimbristylis dichotoma for crude fibre, and potassium, Arundinella setosa for cellulose, Bothriochloa intermedia for Hemicellulose, Dichanthium annulatum for lignin, Andropogon ascinodes for phosphorus during the study period. These species are more palatable in early vegetative stage and considered excellent nutritious fodders. [ABSTRACT FROM AUTHOR]

Cropland (CRL) abandonment is a worldwide phenomenon of land use change with significant impacts on agro‐ecosystems. This research attempts to deepen the analysis of the positive and negative effects arising by focusing on the changes in soil properties and the amount and composition of soil particles detached after several periods of rainfall and the resulting exported sediment that occurs in abandoned areas with natural revegetation compared to CRL. The study was carried out in an agroforestry catchment with a temperate climate, on which CRL and abandoned land with natural vegetation were compared. The soil was sampled along two representative hillslopes integrating elements of the landscape and land use/land covers. Sediments were collected after seven periods in which flood events were recorded during the period July 2016 to December 2017, using artificial‐lawn mats. Soil and sediment composition (texture, soil organic carbon [SOC] and nutrients [total nitrogen and total phosphorous]) under both land uses were assessed and compared and related to rainfall characteristics using principal component analysis. Nutrient enrichment factors in the sediments compared to soils were also evaluated. The results highlight that after abandonment, SOC increased significantly, reaching contents almost three times higher than in CRL. Consequently, soil erodibility decreased, resulting in substantially lower sediment generation after erosive rainfall events. On average, sediment generation was three times lower in abandoned areas than in CRL, despite their steeper slopes. Soil total nitrogen also increased on abandoned lands, reaching values about twice as high as those in CRL. However, total phosphorous content was almost twice as high in CRL than in abandoned land posing a potential risk for water due to higher erosion rates recorded in CRL. The results confirmed the association of phosphorous with smaller particles and also demonstrated the total phosphorous‐SOC link in abandoned land. Furthermore, the effect of rainfall intensity on phosphorous mobilisation was confirmed, whilst nitrogen losses were mainly related to the total amount of rainfall recorded. In a scenario of increasing extreme precipitation, both total amount and increased precipitation intensity may exacerbate water pollution problems following nutrient loss in cultivated areas. This research contributes to identifying the impacts on agroforestry systems within the current context of converting natural areas into cultivated land, whilst in other regions revegetated natural areas are developed from abandoned agricultural land. [ABSTRACT FROM AUTHOR]

Phosphorus (P) is crucial for tree growth and development, and it significantly influences the rhizosphere microbial community. However, the effects of phosphorus addition on the microbial communities in the rhizosphere soil of Idesia polycarpa remain understudied. In this study, two-year-old "Yuji" Idesia polycarpa seedlings were used to investigate the effects of phosphorus fertilization at four different levels of 0 g (control, CK), 0.92 g (low phosphorus, LP), 1.83 g (medium phosphorus, MP), and 2.75 g (high phosphorus, HP) per plant. The fertilizers were applied every 40 days over 120 days. MiSeq high-throughput sequencing of the 16S rRNA and ITS1 genes was employed to analyze the microbial community composition and diversity of bacteria and fungi in the rhizosphere soil under different phosphorus levels. The results showed that compared with CK treatment, the application of phosphorus fertilizer changed the physicochemical properties of the soil. The LP treatment significantly increased the soil pH, while the HP treatment group exhibited the highest soil-available phosphorus (AP) content. LP treatment significantly increased the number of microbial OTUs in the early and rapid growth stages and the richness and diversity of microbial communities. In addition, the bacterial community structure was significantly correlated with soil pH and AP, while the fungal community had no significant effect. The primary metabolic pathway function of bacteria in the rhizosphere soil of Idesia polycarpa seedlings is mainly metabolism, while fungi are mainly biosynthesis. Compared with CK treatment, 20 differential metabolic pathways were screened out in the bacterial community. Only two differential metabolic pathways were screened out in the fungal community by LP treatment at 120 days. In summary, applying low-level phosphate fertilizer is conducive to promoting the diversity of rhizosphere soil microorganisms. Therefore, potted planting of Idesia polycarpa seedlings is more suitable for applying low phosphorus levels. [ABSTRACT FROM AUTHOR]

The COVID-19 pandemic has underscored the critical importance of understanding the intricate relationship between micronutrient levels and disease outcomes. This study explores the impact of Vitamin D, calcium, phosphorus, magnesium, and alkaline phosphatase (ALP) on COVID-19 severity and mortality. The study involves 200 participants (100 COVID-19 patients, 100 controls), we meticulously analyzed micronutrient dynamics. Calcium, phosphorus, magnesium and ALP was measured spectrophotometrically. Vitamin D was measured using Chemiluminescent method. The study reveals that diminished levels of calcium, phosphorus, magnesium, and with elevated ALP, are significantly associated with COVID-19 cases. Whereas the Vitamin D levels in severe group was increased when compared to mild cases but decreased than control group. Disease severity correlated with declining calcium (r = − 0.35, p < 0.01), phosphorus (r = − 0.26, p < 0.05), and magnesium (r = − 0.21, p < 0.05), and increased ALP (r = 0.42, p < 0.001). Post-discharge, calcium (p < 0.05) and phosphorus (p < 0.01) showed positive trends, while ALP (p < 0.001) decreased. Notably, calcium (OR = 0.63, p < 0.05) and ALP (OR = 1.87, p < 0.001) emerged as significant predictors of disease severity. The findings not only illuminate potential therapeutic avenues but also emphasize the need to optimize nutrient levels, including magnesium, for COVID-19 prevention and management. Given the complexities of these relationships, further rigorous exploration, including well-designed trials and understanding underlying mechanisms, is imperative to unravel the dynamics of these nutrient interactions in the context of COVID-19. [ABSTRACT FROM AUTHOR]

Current study was undertaken to investigate the effect of different levels of phosphorous (P) and phosphorous plus nitrogen (NP) fertilizer on nutritive value and in situ digestion kinetics of oat fodder in Nili Ravi buffalo bulls. Four cannulated buffalo bulls were used for in situ screening of oat fodder harvested at 100 days of age sown using different levels of phosphorous and phosphorous plus nitrogen fertilizers. Dry matter (DM) and crude protein (CP) content were higher (p<0.05) in oat fodder supplemented with P and NP fertilizer as compared to control. The supplementation of P fertilizer resulted in decreased NDF and hemicellulose and did not show any effect on ADF content of oat fodder. At 48 h of incubation, in situ digestibility of DM, CP, NDF, ADF and hemicellulose was greater (p<0.05) with medium levels (N20P20 and N25P25) of P and NP fertilizer as compared to control. The extent of DM, CP, NDF, ADF and hemicellulose digestion and rate of degradation was increased in response to P and NP fertilization. However, the lag time of DM, CP, NDF, ADF and hemicellulose of oat fodder was the inverse because it decreased in repose to medium level of P and NP fertilization as compared to control. [ABSTRACT FROM AUTHOR]

Water quality in land-based fish production can be controlled through either instantaneous water exchange or costly wastewater treatment followed by recirculation. Agricultural-aquaculture integration is an excellent alternative technique for reducing nutrient discharge levels, boosting profitability, and converting fish culture wastewater into valuable products. The current study employed a solar energy system to power two separate IMTA-aquaponics systems (Nutrient Film Technique, NFT, and Floating Raft Systems, FRS) for the cultivation of Nile tilapia, African catfish, thin-lipped grey mullet, freshwater crayfish, freshwater mussels, and a variety of vegetables. Tilapia and catfish were fed exclusively on diets under the IMTA system. All wastewater from tilapia and catfish ponds, both dissolved and solid, flows sequentially to ponds containing other cultivated species. The water then flows through the IMTA system's terminal point to the NFT and FRS systems before returning to the tilapia and catfish ponds, allowing complete control of the nutrient flow throughout this entire circular system. Two 147-day production cycles were concluded. The results from the second production cycle are reported. Total biomass gain for aquatic species in the IMTA system was 736.46 kg, compared to 145.49 kg in the tilapia and 271.01 kg in the catfish monoculture systems. The current IMTA system had a cumulative feed conversion ratio (FCR) of 0.90, while the FCRs for tilapia and catfish were 1.28 and 1.42, respectively. Nile tilapia and catfish consumed 571.90 kg of feed containing 25.70 kg of nitrogen (N) and 9.70 kg of phosphorus (P), reflecting, and gaining 11.41 and 3.93 kg of dietary N and P, representing 44.40 and 40.46% dietary N and P retention, respectively. In the IMTA system, the addition of mullet and prawn as detrivores aquatic animals improves dietary N and P utilization efficiency to 59.06 and 51.19%, respectively, while the addition of mussels as herbivore animals improves dietary N and P utilization efficiency to 65.61 and 54.67%, respectively. Finally, using FRS and NFT as hydroponic systems increased dietary N and P efficiency to 83.51% N and 96.82% P, respectively. This study shows that the IMTA-Aquaponic system, as a bio-integrated food production system, can convert the majority of fish-fed residues into valuable products suitable for desert, rural, and urban areas in impoverished and developing countries. [ABSTRACT FROM AUTHOR]

Attapulgite (ATP) is a biocompatible clay mineral that efficiently absorbs water. It is widely used in water treatment due to its environmental friendliness and cost‐effectiveness. This study aimed to develop a volume‐expansion structure‐based attapulgite flocculant (VES‐ATP) using aluminum salt and attapulgite (ATP) under alkaline conditions, specifically for the treatment of water containing low levels of phosphorus. The VES‐ATP was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, and X‐ray photoelectron spectroscopy. The removal of phosphorus by the VES‐ATP was conducted by varying the mass ratio of Al to attapulgite (denoted as RmAl/mATP), ATP dosage, and pH. The results showed that the VES‐ATP had a good expansion and dispersibility in the presence of alkalized aluminum species. The basicity as the molar ratio of OH to Al (0.8 or 1.6) determined the expansion feasibility, and the coverage degree of Al onto ATP, as indicated by the mass ratio of Al to attapulgite (denoted as RmAl/mATP), determined Al flocculation efficiency. Higher values such as RmAl/mATP = 4:1 and 2:1 may result in a better flocculation. Low phosphorus treatment was successfully achieved through Al flocculation and ATP adsorption, including complexation, hydrogen bonding, and electrostatic attraction. As expected, the VES‐ATP generated larger size flocs with a bigger fractal dimension than that with the sole Al flocculation. As a result, the total phosphorus could be reduced to the level below 5 μg/L. It is more efficient in the pH range of 5–9. Overall, the coupling of aluminum and attapulgite has significantly enhanced both purification capabilities of phosphorus. Practitioner Points: Polymeric aluminum‐modified attapulgite was efficient for removal of low phosphorus concentration.Phosphorus concentrations can be reduced to below 5 μg/L.Polymeric aluminum and attapulgite are both safe, and this technology is suitable for water treatment. [ABSTRACT FROM AUTHOR]

Phosphorus (P) discharge from anthropogenic sources, notably sewage effluent and agricultural runoff, significantly contributes to eutrophication in aquatic environments. Stringent regulations have heightened the need for effective P removal technologies in wastewater treatment processes. This paper provides a comprehensive review of current P removal methods, focusing on both biological and chemical approaches. Biological treatments discussed include enhanced biological P removal in activated sludge systems, biological trickling filters, biofilm reactors, and constructed wetlands. The efficiency of microbial absorption and novel biotechnological integrations, such as the use of microalgae and fungi, are also examined. Chemical treatments reviewed encompass the application of metal salts, advanced oxidation processes such as chlorination, ozonation, and the Fenton reaction, as well as emerging techniques including the Electro-Fenton process and photocatalysis. Analytical methods for P, including spectrophotometric techniques and fractionation analyses, are evaluated to understand the dynamics of P in wastewater. This review critically assesses the strengths and limitations of each method, aiming to identify the most effective and sustainable solutions for P management in wastewater treatment. The integration of innovative strategies and advanced technologies is emphasized as crucial for optimizing P removal and ensuring compliance with environmental regulations. [ABSTRACT FROM AUTHOR]

Chronic kidney disease‐mineral bone disorder (CKD‐MBD) is a syndrome commonly observed in subjects with impaired renal function. Phosphate metabolism has been implicated in the pathogenesis of CKD‐MBD and according to the phosphorocentric hypothesis may be the key player in the pathogenesis of these abnormalities. As phosphorous is an essential component for life, absorption from the bowel, accumulation and release from the bones, and elimination through the kidneys are all homeostatic mechanisms that maintain phosphate balance through very sophisticated feedback mechanisms, which comprise as main actors: vitamin D (VD), parathyroid hormone (PTH), calciproteins particles (CPPs), fibroblast growth factor‐23 (FGF‐23) and other phosphatonins and klotho. Indeed, as the renal function declines, factors such as FGF‐23 and PTH prevent phosphate accumulation and hyperphosphatemia. However, these factors per se may be responsible for the organ damages associated with CKD‐MBD, such as bone osteodystrophy and vascular calcification. We herein review the current understanding of the CKD‐MBD focusing on phosphorous metabolism and the impact of phosphate manipulation on surrogate and hard outcomes. [ABSTRACT FROM AUTHOR]

Sulfur (S) is an essential nutrient vital for ideal plant growth and optimal crop yield. Potatoes in sandy soils of northeast Florida often face challenges due to low organic matter content and a high water table. The S application guidelines must be developed to avoid the under and over-application of S and to optimize integrated nutrient management plans for potatoes in Florida's sandy soils. A comprehensive study evaluated the response of S application in potatoes with S uptake, tuber yield, and quality. For 2 years (2021 and 2022), separate field experiments were conducted at three different locations, while location 1 had a trial replicated (Trial 1 and 2). The study was established in a randomized complete block design with four replications, in which five different rates (0, 45, 90, 135, and 180 kg S ha−1, as gypsum [17% S]) were applied at pre-planting. The results of this study show variations in soil-available S concentrations in response to different application rates and sampling times. The dry above-ground biomass production, S uptake, and potato tuber yield remained largely unaffected by S application. However, specific gravity at location 1 reported the response of the S application in interaction with trials. Location-specific effects, compounded by yearly variations, played a significant role, particularly at location 1. External and internal tuber quality showed inconsistent responses to the S application and interactions with trials and year factor. This study reported the significant influence of irrigation water (containing high amounts of SO42−) on the overall experiment. So, these findings underscore the importance of water nutrient concentration in developing crop nutrient recommendations. In conclusion, this study provides valuable insights into the complex relationship between S application, S uptake, and yield in the unique agricultural context of the sandy soils of Florida. [ABSTRACT FROM AUTHOR]

Abstract Purpose Metabolic bone disease of prematurity (MBDP) remains a significant cause of morbidity in extremely premature newborns. In high-risk patients, suspected diagnosis and subsequent treatment modifications, with limitations in terms of sensitivity and specificity, rely on low phosphorus levels and/or high levels of alkaline phosphatase (ALP). We investigated the potential of fibroblast growth factor-23 (FGF23) as an early marker for MBDP when measured at 3–4 weeks of life in at-risk patients. Methods A single-center prospective observational non-interventional study including preterm newborns of both sexes, with a gestational age of less than 32 weeks and/or a birth weight of less than 1500 g. In the standard biochemical screening for MBDP performed between 3 and 4 weeks of life within a nutritional profile, the determination of FGF23 was included along with other clinical and metabolic studies. The study was conducted at Marqués de Valdecilla University Hospital in Santander, Spain, from April 2020 to March 2021. Participants provided informed consent. Biochemical analyses were conducted using various platforms, and follow-up evaluations were performed at the discretion of neonatologists. Patients at high risk for MBDP received modifications in treatment accordingly. The sample was descriptively analyzed, presenting measures of central tendency and dispersion for continuous variables, and absolute numbers/percentages for categorical ones. Tests used included t-tests, Mann‒Whitney U tests, chi-square tests, logistic regressions, Pearson correlation, and ROC curve analysis (IBM SPSS Statistics version 19). Significance level: P

Abstract Water quality in land-based fish production can be controlled through either instantaneous water exchange or costly wastewater treatment followed by recirculation. Agricultural-aquaculture integration is an excellent alternative technique for reducing nutrient discharge levels, boosting profitability, and converting fish culture wastewater into valuable products. The current study employed a solar energy system to power two separate IMTA-aquaponics systems (Nutrient Film Technique, NFT, and Floating Raft Systems, FRS) for the cultivation of Nile tilapia, African catfish, thin-lipped grey mullet, freshwater crayfish, freshwater mussels, and a variety of vegetables. Tilapia and catfish were fed exclusively on diets under the IMTA system. All wastewater from tilapia and catfish ponds, both dissolved and solid, flows sequentially to ponds containing other cultivated species. The water then flows through the IMTA system's terminal point to the NFT and FRS systems before returning to the tilapia and catfish ponds, allowing complete control of the nutrient flow throughout this entire circular system. Two 147-day production cycles were concluded. The results from the second production cycle are reported. Total biomass gain for aquatic species in the IMTA system was 736.46 kg, compared to 145.49 kg in the tilapia and 271.01 kg in the catfish monoculture systems. The current IMTA system had a cumulative feed conversion ratio (FCR) of 0.90, while the FCRs for tilapia and catfish were 1.28 and 1.42, respectively. Nile tilapia and catfish consumed 571.90 kg of feed containing 25.70 kg of nitrogen (N) and 9.70 kg of phosphorus (P), reflecting, and gaining 11.41 and 3.93 kg of dietary N and P, representing 44.40 and 40.46% dietary N and P retention, respectively. In the IMTA system, the addition of mullet and prawn as detrivores aquatic animals improves dietary N and P utilization efficiency to 59.06 and 51.19%, respectively, while the addition of mussels as herbivore animals improves dietary N and P utilization efficiency to 65.61 and 54.67%, respectively. Finally, using FRS and NFT as hydroponic systems increased dietary N and P efficiency to 83.51% N and 96.82% P, respectively. This study shows that the IMTA-Aquaponic system, as a bio-integrated food production system, can convert the majority of fish-fed residues into valuable products suitable for desert, rural, and urban areas in impoverished and developing countries.

Sewage sludge is an important soil conditioner, and source of nutrients with a potential for use in agriculture. However, such benefit needs to be weighed against the risks due to the presence of heavy metals, and other substances that may endanger human health, plants, soil and the ecosystem. This research had the objective of evaluating the agricultural potential of sewage sludge together with the risks that may be present in use. Samples of sewage sludge from seven wastewater treatment plants in Eswatini were analyzed for selected physical and chemical parameters, and heavy metal concentrations using commonly established laboratory procedures. The analysis results indicated that, sludge samples exhibited high organic matter content, cation exchange capacity, macro nutrients such as nitrogen and phosphorus and micro nutrients (trace metal elements) needed for plant growth. Anaerobically digested sludge samples showed higher carbon to nitrogen ratio because of biomass loss in the form of methane and carbon dioxide. The heavy metal concentrations are all within safe limits except the sludge from Matsapha area that had levels of chromium, and nickel above regulatory limits. With respect to their heavy metal contents, most of the sludge samples would qualify as Class A sludge ready to be used as organic fertilizer for agriculture without regular monitoring of heavy metals in the soil to which they are applied. The study results indicated the importance of sewage sludge for their agricultural potential through supplementation of both micro and macro nutrients needed for plant growth, for improving the soil properties such as water holding capacity and permeability, through the increase of organic matter content, and retention of nutrients due to the high cation exchange capacity. All these benefits are realized with sewage sludge being a lower cost alternative to commercial fertilizers. Moreover, the low concentration of heavy metals present in the sludge presents lower risk that may arise in the course of utilizing the sludge as agricultural supplement. [ABSTRACT FROM AUTHOR]

Modern water resource recovery facilities (WRRFs) play a critical role in reducing anthropogenic inputs of phosphorus (P) and nitrogen (N) to aquatic ecosystems, thereby helping to mitigate eutrophication. The enhanced biological phosphorous removal (EBPR) in activated sludge aims to establish a microbial community enriched with active polyphosphate accumulating organisms (PAOs), making it an environmental-friendly and sustainable method for P removal. However, successful application of EBPR relies on both a sufficient supply and appropriate types of organic carbon (C). This review aims to assess the contribution of microorganisms in activated sludge to remove P and discusses efficient C sources that have been used in EBPR. The knowledge gaps within the biological P removal process are evaluated and discussed. Among various organic compounds, acetate and propionate emerge as the most favorable options for achieving stable and efficient P removal by PAOs. However, if these organic compounds are manufactured using fossil fuel-derived materials, their addition will reduce the economic and environmental benefits of EBPR. In comparison, in-line fermentation and side-stream EBPR (S2EBPR) are more advantageous in generating C sources for PAOs by using on-site activated sludge and/or other organic wastes, such as crude glycerol (CG) and food waste (FW). Additionally, future research needs concerning EBPR are discussed to address potential issues related to insufficient C availability in wastewater influent and how microbial competition can be overcome in a sustainable and economical manner. [ABSTRACT FROM AUTHOR]

Horticultural peat extraction can mobilize dissolved organic matter (DOM) and inorganic nutrients (nitrogen and phosphorous) to surface waters, harming aquatic ecosystems and water quality. However, it is uncertain how peat extraction affects solute concentration across hydrological and seasonal conditions and how biogeochemical processing in downstream drainage networks responds. Over two years, we used repeated, spatially extensive sampling in stream networks of two mixed land‐use catchments (<200 km2) on the subhumid interior plains of western Canada. We used random forest models to disentangle the effects of land cover, hydrology, and temperature on water chemistry. Peatlands were the dominant source of DOM to streams, but we detected no substantial effect of peat extraction on DOM concentration or composition. Stream discharge was the most important predictor of DOM composition, with generally humic‐like DOM becoming fresher during snowmelt and summer base flow. We detected no effect from peat extraction on soluble reactive phosphorous (SRP) or nitrate (NO3−). However, total ammonia nitrogen (TAN) was an order of magnitude higher in subcatchments with >40% extracted peatland cover (median: 1.5 mg TAN L−1) compared to catchments with similar intact peatland cover. Mass balance analysis suggested that DOM and inorganic nutrients synchronously attenuated during low flows. During high flows, DOM and inorganic nitrogen were conservatively transported, while SRP was attenuated, likely sorbing to suspended particles. Our study suggests that excess TAN mobilized by peat extraction is utilized in headwaters during low flow but propagates downstream during high flow, with implications for eutrophication that land managers should consider. Key Points: Peat extraction in a subhumid continental climate significantly increased stream ammonia concentration, but not carbon or phosphorusStream hydrology strongly influenced dissolved organic matter (DOM) composition, with low flows shifting DOM from aromatic to aliphaticHydrology affected the downstream persistence of DOM and nitrogen, which attenuated at low flows. Phosphorus was attenuated at all flows [ABSTRACT FROM AUTHOR]

Purpose: Metabolic bone disease of prematurity (MBDP) remains a significant cause of morbidity in extremely premature newborns. In high-risk patients, suspected diagnosis and subsequent treatment modifications, with limitations in terms of sensitivity and specificity, rely on low phosphorus levels and/or high levels of alkaline phosphatase (ALP). We investigated the potential of fibroblast growth factor-23 (FGF23) as an early marker for MBDP when measured at 3–4 weeks of life in at-risk patients. Methods: A single-center prospective observational non-interventional study including preterm newborns of both sexes, with a gestational age of less than 32 weeks and/or a birth weight of less than 1500 g. In the standard biochemical screening for MBDP performed between 3 and 4 weeks of life within a nutritional profile, the determination of FGF23 was included along with other clinical and metabolic studies. The study was conducted at Marqués de Valdecilla University Hospital in Santander, Spain, from April 2020 to March 2021. Participants provided informed consent. Biochemical analyses were conducted using various platforms, and follow-up evaluations were performed at the discretion of neonatologists. Patients at high risk for MBDP received modifications in treatment accordingly. The sample was descriptively analyzed, presenting measures of central tendency and dispersion for continuous variables, and absolute numbers/percentages for categorical ones. Tests used included t-tests, Mann‒Whitney U tests, chi-square tests, logistic regressions, Pearson correlation, and ROC curve analysis (IBM SPSS Statistics version 19). Significance level: P < 0.05. Results: In the study involving 25 at-risk premature newborns, it was found that 20% (n = 5) were diagnosed with MBDP. Three of these patients (60%) were identified as high-risk based on standard biochemical evaluation at 3–4 weeks of age, while the other two patients (40%) were diagnosed in subsequent weeks. However, in all 5 patients, measurement of FGF23 levels would allow for early identification and optimization of treatment before other markers become altered. Low levels of FGF23 at 3–4 weeks, even with normal phosphorus and ALP levels, indicate the need for modifications in nutritional supplementation. Conclusions: MBDP remains a significant concern in extremely premature newborns. Current diagnostic methods rely on limited biochemical markers. Early detection of low FGF23 levels enables timely interventions, potentially averting demineralization. Key points: Question: Is fibroblast growth factor-23 (FGF23) measured at 3–4 weeks of life a reliable early marker for metabolic bone disease of prematurity (MBDP) in at-risk preterm newborns? Findings: This study was a single-center prospective observational noninterventional study conducted in a neonatal unit in Spain. The primary outcome was to investigate FGF23 as a potential early marker for MBDP. The study found that low levels of FGF23 at 3–4 weeks of life, even with normal phosphorus and alkaline phosphatase levels, indicated the need for modifications in nutritional supplementation. FGF23 levels at this time point were found to predict subsequent hypophosphatemic status, making it the most sensitive early marker for MBDP in at-risk preterm newborns. Meaning: Early detection of low FGF23 levels at 3–4 weeks of life can help identify at-risk preterm newborns with MBDP and prompt timely treatment modifications, potentially preventing demineralization and improving outcomes in this vulnerable population. [ABSTRACT FROM AUTHOR]

This study investigates the efficacy of a novel low-cost phosphate adsorbent, denoted as SH-CGCS, derived from coal gasification coarse slag (CGCS) via an alkali activation method. SH-CGCS is a mesoporous material with a specific surface area (64 m2/g) approximately six times larger than CGCS (11 m2/g), which enhances its adsorption capacity compared with CGCS. Furthermore, SH-CGCS achieves a phosphate adsorption capacity of 38.5 mg/g in strongly acidic water (pH 3) and demonstrates robust acid resistance, which makes it particularly effective for phosphate removal from acidic wastewater. Results from coexisting anion experiments affirm the good adsorption selectivity of SH-CGCS for phosphate. Moreover, SH-CGCS exhibits proficiency in treating water containing low phosphate concentrations under flowing conditions. The maximum phosphate adsorption capacity of SH-CGCS calculated using the Langmuir model is 23.92 mg/g, surpassing that of other reported adsorbents. Importantly, saturated SH-CGCS can be regenerated and reused, which contributes to its practical applicability. The adsorption mechanisms of SH-CGCS for phosphate involve ligand exchange, inner-sphere complexation, surface precipitation, and electrostatic adsorption. Thus, this study not only enhances the overall utility of CGCS but also presents a simple and efficient method for removing phosphate. Our findings indicate that SH-CGCS holds considerable potential as a phosphate adsorbent, offering a promising solution for wastewater treatment. [ABSTRACT FROM AUTHOR]

Finding methods, which may improve wheat (Triticumaestivum L.) growth and tolerance under drought stress, are of significance. The hypothesis was plant growth regulators (PGRs) are able to alter wheat physiology in a way so that the plant would be able to resist the stress. Accordingly, PGRs affecting wheat response in drought stress was investigated in greater details. Different PGRs, at different drought levels (80 (S1), 100- (S2) and 120-mm (S3) evaporation from pan class A) affecting wheat biochemical and nutritional properties including nutrient uptake (N, P, K) were tested. Experimental treatments including control (water, T1), and PGRs of gibberellic acid (GA3, 110 mgL−1, T2), salicylic acid (SA, 1.5 mM, T3), and benzyl adenine (60 mgL−1, T4), GA3 + SA (T5), as well as superoxide dismutase (SOD, 5 gL−1, T6) were tested in two different locations. Wheat biochemical and nutritional parameters including grain protein, chlorophyll a and b, leaf relative water at flowering and ripening, wet and dry gluten index, soluble sugars, and grain nutrient uptake were significantly enhanced by PGRs. The grain protein was in the range of 9.90 (S3T1) and 15.01% (S1T5); however, T5 treatment significantly enhanced it at the second (14.47%) and third (13.96%) level of drought. Although the single use of PGR was effective on the alleviation of stress, the combined use of GA3 + SA followed by the single use of SOD, were the most effective ones. Treating wheat plants with the tested PGRs is recommendable to improve wheat biochemical and nutritional properties in the arid and semi-arid areas. [ABSTRACT FROM AUTHOR]

Herein, we present the application of a phosphorus-doped graphitic carbon nitride (P-g-C3N4) for the photodegradation of ciprofloxacin and sulfamethoxazole in water. The photocatalyst was prepared from doping g-C3N4 with phosphorus using different compositions of phosphoric acid (2%, 4%, and 6% w/v). The resultant photocatalysts (2%P-g-C3N4, 4%P-g-C3N4, and 6%P-g-C3N4) were characterized using Fourier transform infrared (FTIR), X-ray diffraction spectroscopy (XRD), scanning electron microscopy–energy-dispersive X-ray (SEM–EDX), and ultraviolet–visible diffuse reflectance mode spectrophotometry (UV–Vis DRS). Photocatalytic degradation studies of the targeted pollutants were performed and monitored using UV–Vis and liquid chromatography coupled with mass spectroscopy (LC–MS). The UV–Vis DRS showed a shift from 2.70 to 2.48 eV in the band gap after doping g-C3N4 with phosphorus. The degradation of sulfamethoxazole by P-g-C3N4 was found to be significantly higher (70%) as compared to g-C3N4 (50%). On the other hand, the removal of ciprofloxacin was found to be 60% for P-g-C3N4, while 50% was found to be the removal efficiency of g-C3N4. The high removal efficiencies were associated with the generated electron holes together with the hydroxyl radicals which played a predominant role in the successful degradation of ciprofloxacin and sulfamethoxazole. Recyclability studies showed that the photocatalyst obtained a high photocatalytic degradation of 65% toward sulfamethoxazole after five cycles. Degradation by-products such as anthralin acid (m/z 307) for ciprofloxacin and monohydroxylated I10 (m/z 269) for sulfamethoxazole were detected using LC–MS. Therefore, P-g-C3N4 serves as a promising photocatalyst for the effective remediation of wastewater generated by pharmaceutical industries. [ABSTRACT FROM AUTHOR]

ABSTRACT: The purpose of the study was to investigate the effects of the reduced dietary calcium (Ca) and phosphorus (P) level and supplementation of 25-hydroxycholecalciferol (25-OHD3) on the expression of vitamin D receptor (VDR) and antimicrobial peptides and gut microbiota of broiler chickens with/without Eimeria challenge. A total of 576 fourteen-day-old broiler chicks were randomly allocated according to a 2 × 2 × 2 factorial design with main effects including Eimeria challenging (125,000 Eimeria acervulina, 25,000 Eimeria maxima, and 25,000 Eimeria tenella), dietary Ca and P levels (0.84% Ca and 0.42% available P or 0.64% Ca and 0.22% available P), and supplementation of 25-OHD3 (3,000 IU/kg) of 6 replicates. Three-way ANOVA was performed, and the effects of 3 main factors and their interactions were investigated. The reduced dietary Ca and P level downregulated cathelicidins 3 (CATH3) in the upper jejunum in the Eimeria challenging condition (interaction; P < 0.05). The reduced dietary Ca and P level decreased the relative mRNA expression of jejunal avian beta defensin 5 (AvBD5) in the Eimeria challenging condition (interaction; P < 0.05). The reduced dietary Ca and P level tended to decrease the relative mRNA expression of jejunal AvBD9 in the Eimeria challenging condition (interaction; P = 0.051). The reduced dietary Ca and P level decreased observed features (alpha diversity parameter for richness) in the upper jejunal microbiota in the Eimeria challenging condition (interaction; P < 0.05). The supplementation of 25-OHD3 decreased the relative abundance of the phylum Bacteroidetes (P < 0.05) and increased the relative abundance of the family Ruminococcaceae (P < 0.05) in the cecal digesta. The supplementation of 25-OHD3 decreased the serum endotoxin level in the Eimeria challenging condition (interaction; P < 0.05). Therefore, the reduced dietary Ca and P level modulated the upper jejunal microbiota via modulating the expression of antimicrobial peptides, and the supplementation of 25-OHD3 favorably modulated the cecal microbiota in broiler chickens with/without Eimeria challenge.

BackgroundDespite the widespread use in ischemic stroke, cancer, and malnutrition, the predictive ability of serum albumin to globulin ratio (A/G) among patients suffering from aneurysmal subarachnoid hemorrhage (aSAH) remains unknown. This study aimed to determine if serum A/G ratio is associated with the occurrence of delayed cerebral ischemia (DCI) after aSAH.MethodsWe retrospectively viewed the medical records of aSAH patients from 08/2017 to 08/2022. Serum albumin and globulin laboratory test results were collected within 24 hours after admission. Serum A/G were dichotomized based on whether the DCI occurred. Logistic regression was used to determine the predictors of DCI. The relationship between serum A/G and the occurrence of DCI was analyzed with receiver operating characteristic(ROC) curve.ResultsA total of 363 eligible patients with aSAH were included in the study, among which DCI occurred in 87 patients(23.97%). Serum A/G[OR=2.720, 95%CI (1.190-6.270), P=0.018], non-surgical[OR=0.228, 95%CI (0.065-0.621), P=0.008], lactate dehydrogenase[OR=1.004, 95%CI (1.000-1.008), P=0.029], P[OR=0.354, 95%CI (0.130-0.926), P=0.038], plasma fibrinogen[OR=1.266, 95%CI (1.019-1.583), P=0.035] were associated with the occurrence of DCI. ROC showed that serum A/G, non-surgical, LDH, P, plasma fibrinogen could predict the occurrence of DCI in aSAH patients with values 0.575, 0.560, 0.602, 0.571 and 0.539 for serum A/G, non-surgical, LDH, P, plasma fibrinogen, respectively.ConclusionsIn conclusion, serum A/G levels are correlated with DCI in individuals with aSAH, and high serum A/G levels on admission may be associated with the occurrence of DCI.

This research delves into the untapped potential of phosphorous and zinc solubilizing rhizobacteria, known as Zn-P isolates, from wheat rhizosphere inceptisols. A total of thirty rhizosphere soil samples were collected, resulting in recovery of forty unique bacterial isolates. After initial screening, out of forty isolates, recovered on the basis of halozone formation on the nutrient agar medium. four were subjected to biochemical and further molecular identification. Four isolates, identified as Bacillus subtilis (Zn-P-1), Pseudomonas aureginosa (Zn-P-2), Staphylococcus aureus (Zn-P-3), and Methylobacterium organophyllum (Zn-P-4) through a set of 16S rRNA primers, forward (5́- GGATGAGCCCGCGGCCTA-3́) and reverse (5́- CGGTGTGTACAAGGCCCGG-3́), exhibited superior solubilization efficiency of phosphorous and zinc These strains were evaluated via in vitro and pot culture assays. The study found that Zn-P-1 demonstrated the highest zinc solubilization (134.87 mg/l) when ZnO was used as the zinc source as compared to ZnCO3 and Zn-EDTA as also highest at 72.32 mg/l in CaH2PO4 and lowest at 14.44 mg/l with KH2PO4 using P sources, thus highlighting the role of Bacillus in zinc and phosphorous activity with substrate type. The inoculation of tri-calcium phosphate (TCP) and ZnCO3, along with Bacillus and Methylobacterium, led to increased phosphorous and zinc solubilization, uptake, and use efficiency, marking these rhizobacteria as potentially beneficial for nutrient enhancement and PGPR activities in wheat crops grown in inceptisols.

Bambara groundnuts (Vigna subterranea (L.) Verdc) provide essential proteins for African farmers, but the yield is largely affected by Alectra vogelii infestation, particularly on nutrient-depleted soils. Laboratory and field investigations were conducted to assess the influence of phosphorus rates and Bambara groundnut genotypes on Alectra vogelii infestation. In the laboratory, a 4 × 26 factorial experiment, varying phosphorus rates (0, 20, 30 and 40 kg ha−1) across 26 genotypes, revealed a significant (p

In this trial, a 3 × 2 factorial design was implemented with dietary treatments consisting of Diet-1 (1.00% calcium [Ca], 0.45% available phosphorus [P]), Diet-2 (0.80% Ca, 0.35% P), and Diet-3 (0.70% Ca, 0.30% P), alongside two phytase levels: 0 and 5,000 FTU/kg. A total of 540 Ross-308 broilers were used. Significant improvements (P

ABSTRACTWe hypothesized that both performance and bone mineralization would show improvements when cholecalciferol supplementation was increased above the recommended NRC (1994) [Nutrient requirements of poultry, 9th rev. ed. Washington, DC: National Academy Press] level. In this study, male Ross 308 chickens (n = 540) were supplemented with diets containing either a low level of 200 IU/kg or a higher dose of 5,000 IU/kg of cholecalciferol (VD). The results showed that during the starter phase, weight gain was significantly higher in VD5000 and the control compared to VD0, with significantly lower feed intake in VD0. Consequently, FCR was significantly better in VD200 and VD5000 compared to VD0. In the finisher phase, weight gain, feed intake, and FCR were significantly higher in VD200 and VD5000 compared to VD0. Tibia ash, Ca, and P were significantly higher in VD5000 compared to VD200. Carcass weight was significantly higher in VD200 and VD5000 compared to VD0. Total tract N retention and GE were significantly higher in VD5000 compared to VD0. The study concludes that cholecalciferol supplementation at 5000 IU/kg enhances growth performance, tibia mineralization, and nutrient digestibility in broilers fed with 30% reduced Ca and P in their diets.

The general trend in biomining (i.e., bioleaching and biooxidation) is the use of media with high concentrations of the nutrients (nitrogen as ammonium, phosphorous as phosphate, and K), which are considered to be essential for microbial growth. The depletion of any of the nutrients would affect negatively the bioleaching (and biooxidation) capacity of the microorganisms, so the formulation of the different media ensures that there is a surplus of nutrients. However, some of these nutrients (e.g., phosphate, K) may be already present in the ore and are made available to the microorganisms when the ore is exposed to the low-pH media used during bioleaching. The effect of phosphate addition (109 mg/L) and depletion on the bioleaching of low-grade sulfidic ore alongside the determination of ammonium (i.e., 25 mg/L, 50 mg/L, 109 mg/L, 409 mg/L, and 874 g/L) requirements were studied. The results of the experiments presented showed that the addition of phosphate did not have any effect on the bioleaching of the low-grade sulfidic ore while the addition of ammonium was necessary to obtain higher redox potentials (>650 mV vs. Ag/AgCl) and higher metal (Co, Cu, Ni, and Zn) dissolutions. Temperature was the factor that shaped the microbial communities, at 30°C, the microbial community at the end of all the experiments was dominated by Acidithiobacillus sp. as well as at 42°C, except when nutrients were not added and Sulfobacillus sp. was the dominant microorganism. At 55°C, DNA recovery was unsuccessful, and at 60°C, the microbial communities were dominated by Sulfolobus sp. In conclusion, the amount of nutrients in bioleaching could be reduced significantly to achieve the redox potentials and metal dissolution desired in bioleaching without affecting the microbial communities and bioleaching efficiencies. [ABSTRACT FROM AUTHOR]

A general approach to the direct deoxygenative transformation of primary, secondary, and tertiary alcohols has been developed. It undergoes through phosphoranyl radical intermediates generated by the addition of exogenous iodine radical to trivalent alkoxylphosphanes. Since these alkoxylphosphanes are readily in situ obtained from alcohols and commercially available, inexpensive chlorodiphenylphosphine, a diverse range of alcohols with various functional groups can be utilized to proceed deoxygenative cross‐couplings with alkenes or aryl iodides. The selective transformation of polyhydroxy substrates and the rapid synthesis of complex organic molecules are also demonstrated with this method. [ABSTRACT FROM AUTHOR]

The effects of calcium phosphate (CaP) materials on macrophage polarization state vary with their physicochemical properties. The study aims to elucidate the impact of phosphate ion‐mediated energy metabolism on M2 macrophage polarization and the corresponding regulatory mechanism. The phosphate ions released from CaP ceramic as bioenergetic factor is identified; its concentration is closely associated with the polarized state. After being taken up by the sodium‐dependent phosphate transporter 1, extracellular phosphate ions produce energy via oxidative phosphorylation by facilitating tricarboxylic acid flux, thereby contributing to M2 macrophage polarization. Further mechanistic analysis reveals that the elevation of the bioenergetic basis can drive macrophage M2 polarization via the AMP‐activated protein kinase‐mammalian target of rapamycin (AMPK‐mTOR) axis. Another regulatory effect is that of the adenosine triphosphate (ATP), a signaling molecule. Intracellular ATP is released into the extracellular space and degraded to adenosine, which serves as a signaling molecule through the A2b adenosine receptor to activate the cyclic adenosine monophosphate (cAMP) pathway, thereby promoting M2 macrophage polarization. Overall, these findings may transform the existing knowledge on cell metabolism and energy homeostasis from bystanders to pivotal factors guiding M2 macrophage polarization and have implications for the future design of biomimetic CaP scaffolds. [ABSTRACT FROM AUTHOR]

Background: Farmers need information regarding soil fertility at every location of their fields to attain a higher level of precision in nutrient management. Nonetheless, the acquisition and processing of soil samples are labor‐intensive and time‐utilizing, and the related cost remains high‐priced to farmers. Artificial intelligence is the most speedily growing area combined into approximately all aspects of human life. Soil macronutrients like nitrogen (N), phosphorous (P), and potassium (K) have a significant role in precision agriculture. There is a huge need for powerful and rapid measurement systems to measure accurately the macronutrients in the soil for optimal crop productivity, especially in site‐specific crop management system, where the application of fertilizer can be regulated spatially with respect to crop demand. Nevertheless, it can present a research direction to design an advanced scheme in order to predict the properties of soil. A portable sensor device is a basic need of an agriculture system for the accurate and rapid monitoring of soil macronutrients. Aim: In this research, the soil nutrients identified from the collected soil samples using optical sensors are evaluated for their accuracy using a deep learning approach. Methods: A deep residual network is exploited for the soil nutrient prediction after augmenting the gathered soil data. Finally, various performance evaluation measures, like mean squared error (MSE), mean absolute error (MAE), and root mean squared error (RMSE), are calculated to detect how accurately the sensor predicted the soil nutrients. Results: From the experimental analysis, it is stated that the proposed model attained low MSE value of 4.59 e−09, the low RMSE value of 6.78 e−05, and the low MAE value of 4.66 e−05 for N prediction. Likewise, the proposed model attained the least MSE value of 1.41 e−05, the least RMSE value of 0.0003, and the least MAE value of 0.0001 for P prediction. Conclusion: Finally, for K prediction, the proposed model achieved the least MSE value of 1.54 e−06, least RMSE value of 1.24 e−03, and the least MAE value of 1.38 e−05. [ABSTRACT FROM AUTHOR]

A phosphorous‐containing benzoxazine, (3‐(4‐hydroxyphenyl)‐3,4‐dihydro‐2H‐benzo[e][1,3]oxazin‐4‐yl)diphenylphosphine oxide (DPOBH), was synthesized and used as flame retardant and co‐curing agent in the presence of 4,4′‐diaminodiphenylsulfone (DDS) for diglycidyl ether of bisphenol‐A (DGEBA) epoxy resin. The flame retardancy, thermal stability, Tg, dielectric properties, and water absorption properties of epoxy resins (EP) were investigated and compared. The incorporation of DPOBH results in ca. 40%–45% increase of limiting oxygen index (LOI) values. UL‐94 V‐0 rating could be achieved for EP/DPOBH1.2 sample with the LOI value of 35.1%. Compared with EP, ca. 47% decrease of peak heat release rate (PHRR) could be obtained for EP/DPOBH1.2. According to the results of cone calorimetry tests (CCT), DPOBH could play roles in both gas and condensed phases. Moreover, the incorporation of DPOBH could reduce the water absorption rate, dielectric constant, and dielectric loss. All results suggest that DPOBH is a type of flame retardant suitable for EP used in electronic fields. [ABSTRACT FROM AUTHOR]

Increased urbanization and industrialization globally have led to the widespread pollution of water bodies (e.g., lakes) by heavy metals (HMs) and nutrients. These pollutants accumulate in water and surface sediments, posing risks to both aquatic organisms and human health. In November 2022, surface sediment samples from three lakes—Lianhua Lake, Mati Lake, and North Lake—were collected to assess nutrient (nitrogen and phosphorous) and HM content. Total N (TN), total P (TP), and HM concentrations were analyzed. The pollution status was evaluated using comprehensive pollution index (FF) methods and the potential ecological risk index (RI) ( E r i ). The results were as follows: (1) Variations in nutrient and HM contents were observed among the three lakes. Lianhua Lake exhibited the highest average TN content (1600 mg/kg), while North Lake had the highest average TP content (2230 mg/kg). The average concentrations of Cd, Hg, and As in the surface sediment surpassed the soil background values of Hubei Province, reaching 1.41, 2.74, and 1.76 times the background values, respectively. Notably, Hg exceeded the standard in Lianhua Lake by 3.39 times, followed by North Lake (2.52 times) and Mati Lake (2.24 times). (2) The FF and potential E r i revealed that the average RI values for Mati Lake, North Lake, and Lianhua Lake were 106.88, 126.63, and 162.18, respectively. These indices categorized the ecological risk levels as moderate, while nutrient salts in the surface water reached a severe pollution level. (3) Correlation and PCA indicated that Cu, Pb, Cd, and Ni were linked to mineral smelting, aquaculture feed, and agricultural fertilizers. Hg and nutrient salts originated from atmospheric deposition of surrounding domestic waste water and traffic exhaust gases. Agricultural activities seemed to contribute to As concentration in the lakes, while Cr has its main origin in the weathering of the rock matrix. [ABSTRACT FROM AUTHOR]

Fluid catalytic cracking (FCC) is a technique that converts heavy-fraction feed into fuel. The FCC catalyst components consist of a composite material made of zeolite, filler, binder, and an active matrix. The active matrix is used as a pre-cracker for the heavy-fraction feed. This study examined the impact of the Si/Al ratio and the addition of phosphorus on the physical properties and activity of the active matrix. The synthesis technique refers to US patent 6723297 B2. The utilized variants consisted of SiO2 ranging from 50 to 80 weight percent and a phosphorous addition ranging from 1 to 2 weight percent. The physical characteristics of the active matrix were assessed using nitrogen physisorption and NH3-Temperature programmed adsorption/desorption techniques. A chemical activity test was conducted using the micro activity test (MAT) method, with vacuum gas oil (VGO) as the feedstock. This test was done in accordance with the ASTM D 5154 - 03 standard. The results indicated that the silica composition in the active matrix is directly related to the average pore diameter but inversely related to the specific surface area. Additionally, the inclusion of phosphorus had a similar impact. The silica-alumina-phosphorous variant containing 75%-wt of SiO2 exhibited the most superior active matrix activity, achieving the maximum acquisition of light cycle oil (LCO) at 33%-wt. [ABSTRACT FROM AUTHOR]

Introduction: The majority of patients in the intensive care unit (ICU) are at risk for at least one electrolyte abnormality and critically ill patients are the most vulnerable population to the effects of suboptimal electrolyte levels. These patients have unique characteristics impacting implications of the electrolyte disturbances and management. Areas Covered: In the ICU, magnesium, potassium, calcium, phosphorous, and sodium are the most commonly encountered electrolytes that can be abnormal in the critically ill. Critical illness both is affected by and affects disturbances of these electrolytes which can result in more severe illness, longer duration of mechanical ventilation, increased dialysis support, longer length of stay, and increased mortality. Often, patients have multiple imbalances that require correction. Removal or mitigation of the cause should be undertaken whenever possible and should be concurrent with any therapeutic management used to correct the electrolyte imbalance. Selection of medication to correct electrolyte imbalances should be undertaken according to available evidence and drug-specific characteristics that impact medication delivery in the critically ill. Electrolyte disturbances, measurements, and corrections are ubiquitous in the ICU. Published literature on the management of electrolytes in the critically ill is limited. Management should consider factors unique to the critically ill when selecting optimal treatment approach and it is essential to individualize treatment to a patient's unique needs and adapt management to the patient's chancing clinical situation. [ABSTRACT FROM AUTHOR]

Phosphorus is a nonrenewable mineral and essential macronutrient for all living organisms. Nevertheless, sustainable utilization of phosphorous is questionable due to the low bioavailability of phosphorus. Nano formulation is a cutting-edge option to increase the bioavailability of phosphorus. If various chemical and physical techniques are available for the nano formulation, the biological route is a great concern due to its simple, eco-friendly and cost-effective nature. Among biological methods, microbial synthesis is a currently focused way due to the smart ability of microbes to synthesis nanoparticles. Microorganisms are consisted of potential materials to synthesize well-defined stable and pure phosphorus-related nanoparticles. Due to the tiny size and stability, microbially synthesized nanoparticles increase the bioavailability of phosphorus. Microbial synthesis of phosphorus-related nanoparticles is not well studied up to now and the area is open for plenty of studies. Deep discovering of the nano formulating mechanism at the genetic level is highly required for the development of nanoparticle synthesis by microbes. Scaling up the technique for mass production is required. Toxicological assessments of phosphorus-related nanoparticles are highly required before the application in the agricultural sector to confirm the safety. If in-depth studies can thorough the knowledge in the field and minimize the existing limitations, microbial synthesis of phosphorus-related nanoparticles would be revolutionized the agricultural sector.

Abstract Introduction The majority of patients in the intensive care unit (ICU) are at risk for at least one electrolyte abnormality and critically ill patients are the most vulnerable population to the effects of suboptimal electrolyte levels. These patients have unique characteristics impacting implications of the electrolyte disturbances and management. Areas Covered In the ICU, magnesium, potassium, calcium, phosphorous, and sodium are the most commonly encountered electrolytes that can be abnormal in the critically ill. Critical illness both is affected by and affects disturbances of these electrolytes which can result in more severe illness, longer duration of mechanical ventilation, increased dialysis support, longer length of stay, and increased mortality. Often, patients have multiple imbalances that require correction. Removal or mitigation of the cause should be undertaken whenever possible and should be concurrent with any therapeutic management used to correct the electrolyte imbalance. Selection of medication to correct electrolyte imbalances should be undertaken according to available evidence and drug-specific characteristics that impact medication delivery in the critically ill. Electrolyte disturbances, measurements, and corrections are ubiquitous in the ICU. Published literature on the management of electrolytes in the critically ill is limited. Management should consider factors unique to the critically ill when selecting optimal treatment approach and it is essential to individualize treatment to a patient’s unique needs and adapt management to the patient’s chancing clinical situation.

Understanding how invasive species respond to changes in abiotic factors and what influences their ability to colonise newly disturbed areas is necessary to predict species expansion, prioritise management efforts, and develop ecological models. We conducted a fully factorial greenhouse experiment using rhizome fragments to examine the effects of four nutrients levels, three soil moisture levels, and two light levels (with five replicates, n = 120) on the growth and resource allocation patterns of Arundo donax, a large‐statured invasive wetland species. We predicted that A. donax's performance‐related traits—traits that directly influence the functionality and fitness of an individual—such as aboveground biomass (AGB), belowground biomass (BGB), net photosynthesis, and average stem height, would be highest under high light, high soil moisture and high nutrient conditions due to the ability of aggressive invasive species to capitalise on greater resource availability. Analyses using general linear mixed‐effects models revealed significant interactions between soil moisture and light. Plants grown in saturated, high light conditions exhibited the highest values for performance‐related traits. However, contrary to predictions, nutrients did not significantly influence these traits. Multivariate analysis of variance demonstrated that nutrients did influence biomass allocation patterns, with plants grown with added nitrogen and phosphorus displaying higher AGB:BGB and stem:leaf ratios. This research highlights A. donax's unique response to nutrient addition and the plasticity of biomass allocation patterns. By understanding how invasive species respond to common abiotic factors, we can better predict their expansion and prioritise management efforts, such as focusing on areas of low overstory shading, while also providing crucial information for ecological model development. [ABSTRACT FROM AUTHOR]

The mid‐Proterozoic (∼1.8–0.8 Ga) ocean‐atmosphere system is hypothesized to have experienced fluctuations in redox conditions with transient oxygenation events. One of these happened at ∼1.4 Ga, and it is speculated that this event may link to the emplacement of large igneous province (LIP) at this time. However, direct evidence for this relationship remains to be proved. Here, we report Hg/TOC, P, and trace element concentrations across the ∼1.4 Ga oxygenation event in the Xiamaling Formation of North China. A prominent increase in Hg/TOC is slightly earlier than that of nutrient contents (especially P), pyrite and TOC abundances, suggesting that this distinct oxygenation event was likely the result of LIP activity at ∼1.4 Ga, which increased nutrient and sulfate supply from continental weathering to the ocean, sustaining elevated primary productivity, organic carbon and pyrite burial. This study indicates that LIP weathering could trigger transient oxygenation events during the mid‐Proterozoic. Plain Language Summary: The mid‐Proterozoic (∼1.8–0.8 Ga) retains persistent low oxygen concentrations in atmosphere and shallow seawater but is occasionally punctuated by transient oxygenation events. Multiple lines of evidence from sedimentary geochemistry have argued a global oxygenation event at ∼1.4 Ga, and speculated that this event was closely related to large igneous province (LIP) activity at this time. Direct link between LIP activity and the oxygenation event, however, has rarely been addressed yet. As mercury in sedimentary rocks has been widely used to trace volcanism, we therefore report Hg/TOC, P, and trace element concentrations across the ∼1.4 Ga oxygenation event. The result shows that a prominent increase in Hg/TOC is clearly recognized in the shales that stratigraphically just underly the layers recording the oxygenation event, suggesting a close causal link between LIP emplacement and the oxygenation event. Accompanied with this Hg/TOC ratio peak, increased nutrient contents (especially P), pyrite and TOC abundances are also observed. Based on these observations, we proposed a model that the Xiamaling oxygenation event was the result of the LIP's emplacement at ∼1.4–1.35 Ga, which increased nutrient and sulfate supply from continental weathering to the ocean, sustaining elevated primary productivity, organic carbon and pyrite burial. Key Points: High Hg/TOC ratios associated with large igneous province (LIP) emplacement were detected in the ∼1.4 Ga Xiamaling Formation shales of North ChinaHigh P content accompanied with this interval indicates enhanced phosphorous input from LIP weathering to the oceanA causal link between the LIP weathering and oxygenation event at ∼1.4 Ga was suggested [ABSTRACT FROM AUTHOR]

From 2021 to 2023, national soybean production has generally decreased due to shrinking cultivation areas. This study aimed to evaluate soybean productivity by applying biochar and regulating water volume. The experiment was conducted in the greenhouse of the Kuningan Soil Laboratory in April–November 2022 using a two-factor Complete Group Randomized Design; the first factor was the biochar dose (0, 10, 15, and 20 tons/ha), and the second factor was the water volume (50% (600 mL), 100% (1200 mL), and 125% (1800 mL). The physical parameters observed were moisture content and texture. The chemical parameters observed include pH, C-organic, cation exchange capacity (CEC), base cations (Ca, Mg, K, Na), N-total, P-total, and P-available. The data were analyzed using analysis of variance followed by Duncan's Multiple Range Test to compare between treatments. The results showed that applying biochar with a dose of 20 tons/ha and 100% water volume can significantly improve soil chemical properties. pH, CEC, Ca-available, Mg-available, K-available, N-available, P-total, P-available, and N-total are markedly affected by the correct biochar dose and water volume. The more applications of biochar, the better the chemical properties of the soil. The provision of water according to needs will increase the availability of nutrients for plants. [ABSTRACT FROM AUTHOR]

Phosphorous (P) removal in wastewater treatment is essential to prevent eutrophication in water bodies. Side‐stream enhanced biological phosphorous removal (S2EBPR) is utilized to improve biological P removal by recirculating internal streams within a side‐stream reactor to generate biodegradable carbon (C) for polyphosphate accumulating organisms (PAOs). In this study, a full‐scale S2EBPR system in a water resource recovery facility (WRRF) was evaluated for 5 months. Batch experiments revealed a strong positive correlation (r = 0.91) between temperature and C consumption rate (3.56–8.18 mg‐COD/g‐VSS/h) in the system, with temperature ranging from 14°C to 18°C. The anaerobic P‐release to COD‐uptake ratio decreased from 0.93 to 0.25 mg‐P/mg‐COD as the temperature increased, suggesting competition between PAOs and other C‐consumers, such as heterotrophic microorganisms, to uptake bioavailable C. Microbial community analysis did not show a strong relationship between abundance and activity of PAO in the tested WRRF. An assessment of the economic feasibility was performed to compare the costs and benefits of a full scale WRRF with and without implementation of the S2EBPR technology. The results showed the higher capital costs required for S2EBPR were estimated to be compensated after 5 and 11 years of operation, respectively, compared to chemical precipitation and conventional EBPR. The results from this study can assist in the decision‐making process for upgrading a conventional EBPR or chemical P removal process to S2EBPR. Practitioner Points: Implementation of S2EBPR presents adaptable configurations, exhibiting advantages over conventional setups in addressing prevalent challenges associated with phosphorous removal.A full‐scale S2EBPR WRRF was monitored over 5 months, and activity tests were used to measure the kinetic parameters.The seasonal changes impact the kinetic parameters of PAOs in the S2EBPR process, with elevated temperatures raising the carbon demand.PAOs abundance showed no strong correlation with their activity in the full‐scale S2EBPR process in the tested WRRF.Feasibility assessment shows that the benefits from S2EBPR operation can offset upgrading costs from conventional BPR or chemical precipitation. [ABSTRACT FROM AUTHOR]

Symbioses with beneficial microbes are widespread in plants, but these relationships must balance the energy invested by the plants with the nutrients acquired. Symbiosis with arbuscular mycorrhizal (AM) fungi occurs throughout land plants, but our understanding of the genes and signals that regulate colonization levels is limited, especially in non-legumes. Here, we demonstrate that in tomato, two CLV3/EMBRYO-SURROUNDING REGION (CLE) peptides, Sl CLE10 and Sl CLE11, act to suppress AM colonization of roots. Mutant studies and overexpression via hairy transformation indicate that Sl CLE11 acts locally in the root to limit AM colonization. Indeed, SlCLE11 expression is strongly induced in AM-colonized roots, but Sl CLE11 is not required for phosphate suppression of AM colonization. Sl CLE11 requires the FIN gene that encodes an enzyme required for CLE peptide arabinosylation to suppress mycorrhizal colonization. However, Sl CLE11 suppression of AM does not require two CLE receptors with roles in regulating AM colonization, Sl FAB (CLAVATA1 ortholog) or Sl CLV2. Indeed, multiple parallel pathways appear to suppress mycorrhizal colonization in tomato, as double mutant studies indicate that SlCLV2 and FIN have an additive influence on mycorrhizal colonization. Sl CLE10 appears to play a more minor or redundant role, as cle10 mutants did not influence intraradical AM colonization. However, the fact that cle10 mutants had an elevated number of hyphopodia and that ectopic overexpression of SlCLE10 did suppress mycorrhizal colonization suggests that Sl CLE10 may also play a role in suppressing AM colonization. Our findings show that CLE peptides regulate AM colonization in tomato and at least Sl CLE11 likely requires arabinosylation for activity. [ABSTRACT FROM AUTHOR]

Agricultural sustainability and land degradation can be monitored through studying soil nutrient dynamics. This study was conducted to investigate the balance and stocks of nitrogen (N) and phosphorus (P) under major land use types and land management (LM) practices in three contrasting agroecological environments in Ethiopia. We employed a nutrient monitoring approach and assessed soil N and P inputs and outputs at watershed and plot scales. Field data were collected from 72 sites representative of the major land uses of the three watersheds for the watershed scale analysis. In addition to the watershed data, 16 experimental runoff plots were established and the response of nutrient balances to improved LM practices for the major land uses, including cropland with three treatments (control, farmyard manure [FYM] and soil bund), grazing land with three treatments (control, exclosure and FYM + exclosure) and bushland with two treatments (control and exclosure). Nutrient balances, flows and stocks varied significantly among agroecological settings, land-use types and LM practices. Severe and negative N balances were found in all agroecological environments, with midland (-70.8) > highland (-61.7) > lowland (-50.3 kg ha-1 yr-1), whereas slightly positive P balances were found in all agro-ecosystems. As a result, the midland (4.2% yr-1) and highland (2.1% yr-1) sites had greater N depletion rates than the lowland site (1.0% yr-1). Our results also demonstrated that applied LM practices improved N balances: by 32%-40% in cropland through FYM and mineral fertilizer, by 7%-18% in grazing land via FYM and exclosure, and by 6%-36% in degraded bushland via exclosure, implying that, implementing FYM and mineral fertilizer for cropland and implementing FYM with exclosure on non-croplands can reverse nutrient depletion in the study sites and in other areas having similar agroecological conditions. [ABSTRACT FROM AUTHOR]

Background and aims: Soil properties and climate influence leaf chemical traits producing intraspecific variation in plants. Studies evaluating their importance in the South American Temperate Forest (STF) species are scarce. This study aimed to evaluate the intraspecific differences in five evergreen species of the rainforest considering two contrasting areas (i.e. temperature and precipitation), linking soil and climate with plants traits. Methods: Soil properties (Corg%, N%, C/N, δ13C, δ15N, pH and temperature), climate variables (mean annual precipitation and temperature [MAP; MAT]) and leaf chemical traits (C%, N% and P%, C/N, N/P, δ13C and δ15N) were measured and compared between two areas in the Northern Patagonia (42°- 44°S). In addition, the relationship of leaf chemical traits with soil and climate was assessed. Results: Significant differences were found in soil (Corg%, C/N and pH; p < 0.05) and climate (p < 0.05), with MAP identified as the most common factor controlling soil properties (Corg%, C/N and δ15N). Intraspecific differences in leaf chemical traits were found between areas, but not in all traits. The most common leaf chemical trait with significant differences was C%. Higher mean C% values were found in the island in plants and soils. High number of correlations (n = 13 correlations; p < 0.05) were found between leaf chemical traits. On the other hand, only MAP was a significant predictor of δ13C in the leaves. Conclusion: The leaf chemical traits variability suggests a species-specific response to the soil and climate conditions, with important influence of precipitation as the most common predictor of soil properties and δ13C in the leaves. [ABSTRACT FROM AUTHOR]

The general trend in biomining (i.e., bioleaching and biooxidation) is the use of media with high concentrations of the nutrients (nitrogen as ammonium, phosphorous as phosphate, and K), which are considered to be essential for microbial growth. The depletion of any of the nutrients would affect negatively the bioleaching (and biooxidation) capacity of the microorganisms, so the formulation of the different media ensures that there is a surplus of nutrients. However, some of these nutrients (e.g., phosphate, K) may be already present in the ore and are made available to the microorganisms when the ore is exposed to the low-pH media used during bioleaching. The effect of phosphate addition (109 mg/L) and depletion on the bioleaching of low-grade sulfidic ore alongside the determination of ammonium (i.e., 25 mg/L, 50 mg/L, 109 mg/L, 409 mg/L, and 874 g/L) requirements were studied. The results of the experiments presented showed that the addition of phosphate did not have any effect on the bioleaching of the low-grade sulfidic ore while the addition of ammonium was necessary to obtain higher redox potentials (>650 mV vs. Ag/AgCl) and higher metal (Co, Cu, Ni, and Zn) dissolutions. Temperature was the factor that shaped the microbial communities, at 30°C, the microbial community at the end of all the experiments was dominated by Acidithiobacillus sp. as well as at 42°C, except when nutrients were not added and Sulfobacillus sp. was the dominant microorganism. At 55°C, DNA recovery was unsuccessful, and at 60°C, the microbial communities were dominated by Sulfolobus sp. In conclusion, the amount of nutrients in bioleaching could be reduced significantly to achieve the redox potentials and metal dissolution desired in bioleaching without affecting the microbial communities and bioleaching efficiencies.