Search

The study focused on the impact of the insoluble fraction of Persian gum–sodium alginate and a blend of the insoluble fraction of Persian gum–sodium alginate (IFPG‐Al) with whey protein isolate (WPI) on sprayed Ziziphus jujuba extract (JE) powder. The addition of whey protein led to powders with higher moisture (10%), higher solubility (99.19%), and lower powder yield (27.82%). The powders fabricated with WPI depicted the best protection of polyphenolic compounds (3933.4 mg/L) and the highest encapsulation efficiency activity (74.84%). Additionally, they had a higher Tg (62.63°C), which indicates more stability of the powders during shelf life. The sphericity of the majority of the particles was noticeable in powders, but multi‐sided concavities were visible in the protein‐containing particles. Based on the particle size's results, IFPG‐Al/WPI capsules fabricated relatively smaller particles (2.54 μm). It can be acknowledged that the presence of protein in particles can bring fruitful results by preserving valuable bioactive compounds. [ABSTRACT FROM AUTHOR]

Probiotics are defined as “live microorganisms that provide health benefits to the host when administered in adequate amounts.” Probiotics have beneficial effects on human health, including antibacterial activity against intestinal pathogens, regulation of blood cholesterol levels, reduction of colitis and inflammation incidence, regulation of the immune system, and prevention of colon cancer. In addition to probiotic bacteria, some phenolic compounds found in foods we consume (both food and beverages) have positive effects on human health. p-coumaric acid (p-CA) is one of the most abundant phenolic compounds in nature and human diet. The interactions between these two different food components (phenolics and probiotics), resulting in more beneficial combinations called synbiotics, are not well understood in terms of how they will affect the gut microbiota by promoting the probiotic properties and growth of probiotic bacteria. Thus, this study aimed to investigate synbiotic relationship between p-CA and Lactobacillus acidophilus LA-5 (LA-5), Lacticaseibacillus rhamnosus GG (LGG). Probiotic bacteria were grown in the presence of p-CA at different concentrations, and the effects of p-CA on probiotic properties, as well as its in vitro effects on AChE and BChE activities, were investigated. Additionally, Surface analysis was conducted using FTIR. The results showed that treatment with p-CA at different concentrations did not exhibit any inhibitory effect on the growth kinetics of LA-5 and LGG probiotic bacteria. Additionally, both probiotic bacteria demonstrated high levels of antibacterial properties. It showed that it increased the auto-aggregation of both probiotics. While p-CA increased co-aggregation of LA-5 and LGG against Escherichia coli, it decreased co-aggregation against Staphylococcus aureus. Probiotics grown with p-CA were more resistant to pepsin. While p-CA increased the resistance of LA-5 to bile salt, it decreased the resistance of LGG. The combinations of bacteria and p-CA efficiently suppressed AChE and BChE with inhibition (%) 11.04–68.43 and 13.20-65.72, respectively. Furthermore, surface analysis was conducted using FTIR to investigate the interaction of p-coumaric acid with LA-5 and LGG, and changes in cell components on the bacterial surface were analyzed. The results, recorded in range of 4000 –600 cm-1 with resolution of 4 cm-1, demonstrated that p-CA significantly affected only the phosphate/CH ratio for both bacteria. These results indicate the addition of p-CA to the probiotic growth may enhance the probiotic properties of bacteria. [ABSTRACT FROM AUTHOR]

Cellular slime molds are excellent model organisms in the field of cell and developmental biology because of their simple developmental patterns. During our studies on the identification of bioactive molecules from secondary metabolites of cellular slime molds toward the development of novel pharmaceuticals, we revealed the structural diversity of secondary metabolites. Cellular slime molds grow by feeding on bacteria, such as Klebsiella aerogenes and Escherichia coli, without using medium components. Although changing the feeding bacteria is expected to affect dramatically the secondary metabolite production, the effect of the feeding bacteria on the production of secondary metabolites is not known. Herein, we report the isolation and structure elucidation of clavapyrone (1) from Dictyostelium clavatum, intermedipyrone (2) from D. magnum, and magnumiol (3) from D. intermedium. These compounds are not obtained from usual cultural conditions with Klebsiella aerogenes but obtained from coincubated conditions with Pseudomonas spp. The results demonstrate the diversity of the secondary metabolites of cellular slime molds and suggest that widening the range of feeding bacteria for cellular slime molds would increase their application potential in drug discovery. [ABSTRACT FROM AUTHOR]

Ber fruit (Zizyphus mauritiana Lamk) contains abundant polyphenols and is highly susceptible to enzymatic browning after harvest. In order to reduce browning and maintain fruit quality of ber fruit, a factorial experiment was conducted in a completely randomized design with four replications. Experimental treatments included control, Zataria multiflora essential oil (ZEO) (0.5%), ZEO (1%), Aloe vera gel -based (20%) enriched with sesame oil 1%, honey 1% (ASH), ASH + ZEO (0.5%), ASH + ZEO (1%) and storage time (0, 7, 14, 21 and 28 days) at 6 ± 1°C and relative humidity of 90±5%. The results showed that edible coating application had significant effect on the quality characteristics of the ber fruit during storage. The combination of ASH with ZEO (0.5%), as an edible coating, significantly alleviated enzymatic browning by reducing PPO and POD activities on fruit during 28 days of storage. Fruits treated with ASH with ZEO (0.5%) reduced browning index (70%) compared to the control; while increased total phenol content and antioxidant activity (18.14%, and 31.16%, respectively) compared to the control after 28 days of storage. Also, ASH in combination with ZEO (0.5%) treatment decreased electrolyte leakage and malondialdehyde (19.7%, and 17.8%, respectively) compared to the control after 28 days of storage. These results indicated that ASH edible coating in combination with ZEO (0.5%) can be a useful method for delaying ripening and senescence, extending the storage life of ber fruit. [ABSTRACT FROM AUTHOR]

Mushrooms have been an acclaimed food for their unique flavor and medicinal properties since ancient times. Modern research shows that mushrooms are rich in various nutrients and biologically active substances. In recent years, researchers have become increasingly interested in mushrooms because they contain important secondary metabolites, such as phenolic compounds with significant bioactive properties. This review introduces the nutritional components and secondary metabolites in mushrooms, focusing on the bioactive functions and potential applications of mushroom polyphenols. Finally, the current challenges and future research trends of mushroom polyphenols are briefly discussed. In the aspect of nutritional value, mushrooms are high in protein and insoluble fiber, while low in fat and sodium, making them a low-energy, healthy food. Mushrooms contain a large amount of beneficial bioactive substances for health, including phenolic compounds, as well as tocopherols, terpenoids, and phytosterols. Mushroom polyphenols have antioxidant, anti-inflammatory, anti-cancer, anti-tyrosine, antihyperglycemic, and other biological activities beneficial to human health and medical applications, especially in the various degenerative disease and cancer treatments. However, based on the properties of phenolic compounds, research and development in commercial applications still face many issues that need to be addressed by researchers. [ABSTRACT FROM AUTHOR]

Caulerpa lentillifera (sea grapes) stands out for its nutritional profile and associated health advantages. Seaweeds, including sea grapes, exhibit diverse chemical compositions that are influenced by environmental conditions. This study aimed to compare the chemical composition, metabolite content, antioxidant activity and a-glucosidase inhibition of sea grapes collected at different sites (Phetchaburi and Trang) and at different times during the month of January, April and July 2022. Both collection times and sites have influenced all assessed parameters. The sea grapes collected from Trang had a significantly higher amount of metabolite, antioxidant activity and a-glucosidase inhibitory activity than those from Phetchaburi at p < 0.001. The highest amount of phenolic compound (93.76 ± 2.39 mg GAE/g extract) and reducing power (EC50 = 25.86 ± 0.20 mg/mL) occurred in sea grapes in April month, while those collected in January and April had high DPPH and ABTS radical scavenging (EC50 < 7.50 mg/mL). The highest inhibitory activity against a-glucosidase (EC50 = 19.27 ± 0.40 mg/mL) was found in the sea grapes collected in January. The LC-QTOF MS/MS result of sea grape extract indicated similar compounds in amino acids, fatty acids, lipids, nucleic acids, carboxylic acids, cyanogenic glucosides, steroids, terpenoids and vitamins. The disparity in phenolic profiles between Phetchaburi (9) and Trang (14) sea grapes was related to higher phenolic compound and flavonoid contents, coupled with antioxidant properties in Trang. These findings supported the possible use of sea grapes from Trang as a reservoir of bioactive compounds. [ABSTRACT FROM AUTHOR]

This study aims to investigate the properties of B. griseipurpureus Corner concerning its efficacy against various bacterial strains and its antioxidant capacity, along with its phytochemical composition. The antibacterial activity was evaluated using the agar well diffusion method, revealing pronounced efficacy against gram-negative bacteria, particularly noteworthy against E. coli ESBL182, known for its antibiotic resistance. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of B. griseipurpureus Corner extract against E. coli ESBL182 were determined to be 75 and 300 mg/ml, respectively. The extract exhibited bacteriostatic activity by inhibiting cell growth, leading to a reduction in optical density at 600 nm. Additionally, examination of the supernatant from untreated and treated E. coli ESBL182 cells with B. griseipurpureus Corner extract indicated absorbance values of 0.088 and 0.248 at 260 nm, and 0.045 and 0.286 at 280 nm, respectively. Further investigation utilizing a scanning electron microscope revealed alterations in the morphology of treated cells, which displayed elongation and fragmentation, in contrast to untreated cells. The DPPH assay indicated that the IC50 of B. griseipurpureus Corner extract was 31.22 mg/ml. The IC50 value obtained from the ABTS assay was 47.31 mg/ml. Additionally, the FRAP assay revealed that the concentration of ascorbic acid equivalent in B. griseipurpureus Corner extract was 1.06 mg/g crude extract. Phytochemical analysis, conducted using a spectrophotometer at wavelengths of 750 nm and 510 nm, respectively, indicated phenolic and flavonoid contents of 0.22 mg gallic acid/g fresh weight and 3.23 mg quercetin/g fresh weight in the extract. [ABSTRACT FROM AUTHOR]

The main physiological and biochemical characteristics and elemental composition of three lichen species of the genus Hypogymnia (Nyl.) Nyl. in one habitat were studied using spectroscopic methods. The model species were placed in the following order of decreasing degree of anthropotolerance: H. physodes (L.) Nyl. → H. tubulosa (Schaer.) Hav. → H. vittata (Ach.) Parrique. The contents of chlorophylls a and b, phenolic compounds, pheophytinization quotient, and antiradical activity were determined by a spectrophotometric method. The antioxidant activity was determined by an amperometric method. The physiological and biochemical parameters for each of the three species corresponded to those for background ecotopes. These parameters and the integrity of the system of correlations between the parameters were lower in species with a low degree of anthropotolerance. Twenty-three elements were found in thalli of the model species using atomic emission spectroscopy with inductively coupled plasma. They included macro- and microelements, heavy metals, and metalloids. The maximum concentrations of most elements were found in H. vittata; the minimum concentrations, in H. physodes. An analysis of the interaction between the physiological and biochemical characteristics and the contents of the elements indicated the presence of a complex system of correlations in each species. Differences in this system of correlations may have been due to the specific composition of secondary metabolites, which determine the features of adaptive reactions. The use of various optical spectroscopy methods enabled an evaluation of not only the functional specificity of the studied species but also its connection to their anthropotolerance level. Low resistance to anthropogenic influences was combined with lower coordination of physiological and biochemical characteristics and low integrity of the system of correlations. The most vulnerable species H. vittata had the minimal values of the main functional parameters, a poorer correlation of them with the elemental composition, and higher concentrations of some toxic elements. The use of a complex analysis of the physiological and biochemical characteristics and elemental compositions using various spectral methods was crucial for the bioindication and ecological physiology of lichens. [ABSTRACT FROM AUTHOR]

Manganese toxicity is a major obstacle to agriculture in acid soils. Dark pericarp disease (DPD) is a newly spread physiological disorder induced by excess Mn in litchi, leading to undesirable fruit appearance and substantial economic loss. In this work, broadcast of alkaline soil conditioner in winter, followed by foliar sprays of ascorbic acid and sulfur solution at fruit development, was adopted to examine the effect of these combinations on DPD alleviation in a litchi orchard, with DPD morbidities of 70~85% in recent ten years. The combination of soil conditioner broadcast and foliar water spray was used as the control. At harvest, DPD incidence was significantly decreased by sulfur spray (3.3 ± 1.0%) and slightly reduced by ascorbic acid spray (10.7 ± 8.0%) compared to the control (12.9 ± 7.6%). Soil pH and available Mn were significantly increased and reduced by the soil conditioner broadcast. Sulfur spray significantly inhibited Mn uptake but enhanced the accumulation of Mg, Ca, sugars and cyanidin-3-rutinoside in the pericarp, leading to improved fruit pigmentation. Antioxidase activities were regulated to resist Mn stress by sulfur spray. The spray of ascorbic acid could not mitigate DPD as expected, probably due to the dose used. Conclusively, this study provides a practicable approach to mitigate Mn phytoavailability in acid soils. [ABSTRACT FROM AUTHOR]

Sabah is the main seaweed producer in Malaysia especially red seaweeds which are commercially cultivated for the production of food gum known as carrageenan. Seaweeds are also high in phytochemical such as antioxidant compounds. Three seaweeds species from the Semporna Seawater, Sabah, namely Kappaphycus alvarezii, Caulerpa lentillifera and Sargassum polycystum were chosen for this study for the analysis of their antioxidant activities. K. alvarezii species is commercially cultivated whereas the other two (C. lentillifera and S. polycystum) are wild species. All seaweeds species underwent drying process in a cabinet dryer prior to the analyses. Six types of solvents which were water, ethanol, methanol, chloroform, ethyl acetate and hexane were used for the extraction process. Every concentrated, extracted solutions were then measured for their antioxidant activities based on total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) and β-carotene bleaching assay. Results showed that TPC of S. polycystum was significantly higher (p<0.05) than K. alvarezii but not significantly different (P>0.05) from C. lentillifera. Even though the extraction yield of water was the highest, but the greatest antioxidant activity values were seen in methanolic extraction in comparison to the other solvents in TPC, DPPH and FRAP. This indicated that most of the antioxidant compounds in all the three seaweed samples are polar. However, high antioxidant activity in terms of β-carotene bleaching assay were seen in other solvent extractions, which were chloroform, hexane and ethyl acetate. This indicated that seaweed also contain non-polar antioxidant compounds such as β-carotene. Overall, the best extraction method of antioxidant compounds in seaweed samples was using methanol as the extractant. [ABSTRACT FROM AUTHOR]

This study investigated the production of Sangyod rice bran hydrolysate (SYRB) from Sangyod rice, focusing on incubation times (1, 3, and 5 h) and alcalase enzyme concentrations (0, 0.7, and 1% v/v). The results demonstrated a concentration-dependent relationship: higher alcalase concentrations increased hydrolysate yield. Prolonged incubation, especially with alcalase, enhanced substrate breakdown, further increasing hydrolysate production. The degree of hydrolysis, reflecting peptide bond cleavage, depended on both incubation time and enzyme concentration, emphasizing the role of enzyme activity in efficiency. Moreover, color analysis (L*, a*, b*) and color difference (∆E) revealed intricate changes from enzymatic hydrolysis. Proximate composition analysis showed higher protein and lipid content with increased enzyme concentration and longer incubation times, whereas ash content varied with both factors. Hydrolysate powders exhibited higher moisture content than raw rice bran, indicating the impact of the hydrolysis process. The study also explored SYRB's antioxidant properties and cytotoxicity, which were sensitive to incubation time and alcalase concentration. Longer incubation increased DPPH scavenging activity, with the highest efficacy at 3 h. Meanwhile, ABTS scavenging displayed a delicate balance with alcalase concentration. The cytotoxicity study of SYRB revealed that all concentrations of SYRB were non-toxic to C2C12 cells, with cell viability values exceeding 70%. [ABSTRACT FROM AUTHOR]

Phenolic compounds are known to have a significant effect on human defense system due to their anti-inflammatory efficacy. This can slow down the aging process and strengthen the human immune system. With the growing interest in green chemistry concept, extraction of phenolic compounds from plants has been geared towards a sustainable path with the use of green and environmentally friendly solvents such as natural deep eutectic solvents (NADES). This review discusses both the conventional extraction and the advanced extraction methods of phenolic compounds using NADES with focus on microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) techniques ensued by a rationale comparison between them. Employing choline chloride-based natural deep eutectic solvents (NADES) is highlighted as one of the promising strategies in green solvent extraction of phenolic compounds in terms of their biodegradability and extraction mechanism. The review also discusses assistive extraction technologies using NADES for a better understanding of their relationship with extraction efficiency. In addition, the review includes an overview of the challenges of recovering phenolic compounds from NADES after extraction, the potential harmful effects of NADES as well as their future perspective. [ABSTRACT FROM AUTHOR]

This study reports on the synthesis of graphene oxide layered bismuth telluride and grafted by 4-aminobenzoic acid, as a novel nanoadsorbent, and its potential for removal of bisphenol A. The nanoadsorbent was characterized by different techniques, such as Fourier transform infrared, X-ray diffraction spectroscopy, thermogravimetric analysis, scanning electron microscopy, and energy-dispersive X-ray. The sorption equilibrium was well fitted on the non-linear Langmuir isotherm model (R2= 0.9782). The kinetic models show that bisphenol A sorption fits the non-linear pseudo-second-order model (R2=0.9999). The maximum sorption capacity of 41.56 mg g-1 was obtained for nanoadsorbent under a pH of 5, nanoadsorbent dosage of 0.01 g, a temperature of 25 °C, and a contact time of 15 min. The nanoadsorbent was recyclable by 80% regeneration efficiency after the fifth cycle of usage. This work demonstrated that the synthesized nanoadsorbent was a novel and promising adsorbent for the removal of bisphenol A with an efficiency of >91% from the environmental water and industrial sewage samples. [ABSTRACT FROM AUTHOR]

Purslane (Portulaca oleracea L.) has been known as a medical herb with valuable nutritional and pharmacological properties. Herein, water, methanol, and acetone extracts were prepared from two morphologically different purslane samples, thereafter their content of β-carotene, chlorophyll a and b, total phenolic compounds (TPC), total flavonoids, and total flavonols was evaluated. Additionally, DPPH• scavenging activity and ferrous iron (Fe+2) chelating activity of these extracts were also measured. The acetone extract had the highest amounts of β-carotene (14.16 mg g-1 DW), chlorophyll a (40.46 mg g-1 DW), chlorophyll b (9.94 mg g-1 DW), TPC (51.01 mg GAE g-1 DW), flavonoids (133.23 mg RE g-1 DW), and flavonols (46.94 mg RE g-1 DW). Whereas the lowest values were observed in the water extract, as 0.03 mg g-1 DW, 0.03 mg g-1 DW, 0.06 mg g-1 DW, (34.24 mg GAE g-1 DW, 10.98 mg RE g-1 DW, 3.28 mg RE g-1 DW, respectively. The acetone extract also showed higher DPPH• scavenging activity (IC50 = 57.23 μg mL-1) compared to methanol (IC50 = 65.80 μg mL-1) and water (IC50 = 71.47 μg mL-1) extracts. Regarding the ferrous iron (Fe+2) chelating activity, methanol extract exhibited the highest value followed by water and acetone extracts, being 12.78, 10.20 and 6.08%, respectively. A positive correlation was detected between the DPPH• scavenging activities of the purslane extracts and their content of TPC, flavonoid, flavonol, β-carotene, chlorophyll a, and chlorophyll b. On the other hand, there was no significant correlation between chelating activity of the extracts and their content of β-carotene, chlorophyll a, TPC, flavonoids, flavonols, and DPPH scavenging activity. Purslane extracts could be suggested as a natural antioxidant substance for many pharmaceutical and food applications. [ABSTRACT FROM AUTHOR]

Polygonum cognatum Meissn, a perennial herbaceous belonging to the Polygonaceae family, is an aromatic plant. High‐performance liquid chromatography/diode array detector method was developed and validated for the phytochemical analysis of the plant. Also, various methods were used to investigate the antioxidant, antimicrobial, and cytotoxic activities of the methanolic extracts. Antioxidant activities were researched by 2,2′‐diphenyl‐1‐picrylhydrazyl and cupric reducing antioxidant capacity methods. Among the tested standard microbial strains, Candida albicans was found to be more sensitive with a 24.60 ± 0.55 mm inhibition zone according to the diffusion tests. In the microdilution tests, the minimum inhibitory concentration and minimum bactericidal/fungicidal concentration values were 4.75 and ≥ 4.75 mg/mL, respectively, for all tested pathogens. Human colon carcinoma cells were used to investigate cytotoxicity by using 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide analysis (IC50 = 2891 μg/mL for Plant A, IC50 = 3291 μg/mL for Plant B). Molecular docking and absorption, distribution, metabolism, excretion, and toxicity analysis were used to explain inhibition mechanisms of major phenolic compounds of plants against Tankyrase 1, Tankyrase 2 enzymes, and deoxyribonucleic acid gyrase subunit B and found compatible with experimental results. [ABSTRACT FROM AUTHOR]

To determine the free-radical scavenging activity and total phenolic content of the ethanol extract of Poinsettia leaves (Euphorbia pulcherrima Willd.) from Lombok Island, we used the 1,1-diphenyl-2-picrylhydrazyl method and expressed the total phenolic content as gallic acid equivalent (GAE) per gram of ethanol extract. The results show the strong antioxidant activity (79.77 ppm) of the ethanol extract of red Poinsettia leaves and the moderate antioxidant activity of green Poinsettia leaves (118.350 ppm). The ethanol extracts of red and green Poinsettia leaves possessed an average of 63.276 ± 0.228 and 22.715 ± 0.090 mg GAE/g extract total phenolic contents, respectively. In addition, the ethanol extracts of red and green Poinsettia leaves exhibited moderate and strong free-radical inhibitory activities, respectively. These findings indicate that color affects the antioxidant ability of leaves and their phenolic content. [ABSTRACT FROM AUTHOR]

Type 2 diabetes mellitus (T2DM) is a global problem with increasing prevalence. The current treatments have made an immense progress with some side effects, such as drug resistance, acute kidney toxicity, and increased risk of heart attack. Banana (Musa spp.) peel comprises 40% of banana fruit contains high phenolic compounds whilst some studies have suggested a correlation between phenolic compounds and antidiabetic activity. One of the novel protein targets that has been identified as a potential anti-diabetic treatment is PTP1B (PDB ID:2NT7). Therefore, this study aimed to screen the potential PTP1B inhibitor for antidiabetic treatment from phenolic compounds in banana peel. QSAR, molecular docking, ADME-Tox, and molecular dynamics analysis were deployed to examine forty-three phenolic compounds in banana peel. Eighteen ligands were screened by QSAR analysis and eight of them had a lower binding energy than the standard (ertiprotafib) in molecular docking, with urolithin A and chrysin were the lowest. Both passed Lipinski's rule of five, had a good intestinal absorption, and no blood-brain barrier penetration, however, their mutagenicity, carcinogenicity, and irritation to the skin and eyes were still in questions. Molecular dynamics analysis found both of them were in a stable conformation with PTP1B. This study suggested a potential of urolithin A and chrysin as PTP1B inhibitor for antidiabetic treatment. Additionally, further experimentation is required to validate this finding. [ABSTRACT FROM AUTHOR]

In northern peatlands, reduction of Sphagnum dominance in favour of vascular vegetation is likely to influence biogeochemical processes. Such vegetation changes occur as the water table lowers and temperatures rise. To test which of these factors has a significant influence on peatland vegetation, we conducted a 3‐year manipulative field experiment in Linje mire (northern Poland). We manipulated the peatland water table level (wet, intermediate and dry; on average the depth of the water table was 17.4, 21.2 and 25.3 cm respectively), and we used open‐top chambers (OTCs) to create warmer conditions (on average increase of 1.2°C in OTC plots compared to control plots). Peat drying through water table lowering at this local scale had a larger effect than OTC warming treatment per see on Sphagnum mosses and vascular plants. In particular, ericoid shrubs increased with a lower water table level, while Sphagnum decreased. Microclimatic measurements at the plot scale indicated that both water‐level and temperature, represented by heating degree days (HDDs), can have significant effects on the vegetation. In a large‐scale complementary vegetation gradient survey replicated in three peatlands positioned along a transitional oceanic–continental and temperate–boreal (subarctic) gradient (France–Poland–Western Siberia), an increase in ericoid shrubs was marked by an increase in phenols in peat pore water, resulting from higher phenol concentrations in vascular plant biomass. Our results suggest a shift in functioning from a mineral‐N‐driven to a fungi‐mediated organic‐N nutrient acquisition with shrub encroachment. Both ericoid shrub encroachment and higher mean annual temperature in the three sites triggered greater vascular plant biomass and consequently the dominance of decomposers (especially fungi), which led to a feeding community dominated by nematodes. This contributed to lower enzymatic multifunctionality. Our findings illustrate mechanisms by which plants influence ecosystem responses to climate change, through their effect on microbial trophic interactions. [ABSTRACT FROM AUTHOR]

Puerarin, which was used as a natural isoflavone in this study, was obtained from the Kudzu plant. The inhibitory effect of puerarin on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes was investigated. The IC50 values of AChE (177.73 nM) and BChE (315.07 nM) were determined. The binding mechanism of puerarin, which has a high inhibitory effect on the active site of the enzyme, was demonstrated by the molecular docking method. The antioxidant activity was evaluated for the same molecule. For this purpose, Fe3+‐Fe2+ reducing capacity, ABTS radical scavenging activity, CUPRAC method, DPPH⋅ scavenging activity and DMPD radical scavenging method were used. The percentage (%) inhibition amount of puerarin molecule was calculated as 99.33 % for ABTS radical scavenging activity, which is the more preferred method for natural products. The absorbtion value of iron reduction of Puerarin was 0.122±0.010, and the value of copper reduction was 0.633±0.026. Our research efforts include uncovering the potent antioxidant potential of puerarin and, simultaneously, a comparative study of tacrine, primarily emphasizing its inhibitory effects against AChE and BChE enzymes. The puerarin is expected to be a guide for drug discovery against many problems, especially oxidative cell damage in Alzheimer's disease, mitochondrial dysfunction (MD), cancer, and cardiovascular diseases. [ABSTRACT FROM AUTHOR]

Pink-pigmented facultative methylotrophs bacteria are a plant's surface inhabitant, especially at the leaf. They are known as Methylobacterium species. The antioxidant activity, phenolic compounds, and level of toxicity of this bacteria pigment have been studied. Recently, no previous research focused on the same bacterium found in Melicope lunu-ankenda (Gaertn.) T. G. Hartley, which is a component of the Malaysian ulam leaf. This study employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, along with total phenolic content determination to assess the antioxidant activities of the methanolic and ethanolic pigment extract. Additionally, the consumption safety level of the pigment extract used brine shrimp lethality assay. From these findings, ethanolic pigment extract has a higher antioxidant capacity than methanolic extract. The DPPH half-maximal inhibitory concentration (IC50) value of methanolic pigment extract is higher than ethanolic extract (0.72 ± 0.04 mg/ml), but the IC50 value is vice versa for ABTS (4.59 ± 2.17 mg/ml). Furthermore, ethanolic extracts have a high FRAP assay value (1.09 ± 0.19 mg/mg of trolox equivalent at 0.78 mg/ml sample) and phenolic content (1.39 ± 0.07 mg/mg of gallic acid equivalent at 0.78 mg/ml sample) compared to methanolic pigment extracts. Fortunately, the methanolic and ethanolic pigment extract's lethal concentration values (4.52 and 9.94 mg/ml) are considered safe for food application since their toxicity level is higher than 1 mg/ml. [ABSTRACT FROM AUTHOR]

Seaweeds are rich in phenolic compounds such as phlorotannins and have antioxidant, antibacterial, and anti-inflammatory activities. As exogenous bioactive compounds, seaweed polyphenols exist in the form of aglycone, esters, glycosides, and polymers. Most of them are not directly absorbed in the human digestive tract but undergo extensive modifications by digestive enzymes or bacteria before absorption or excretion. Up-to-date in vitro and in vivo seaweed polyphenol bioaccessibility studies have been reviewed, outlining seaweed polyphenols, especially phlorotannins, have low oral bioaccessibility, bioavailability, and high bioactivity paradox. Moreover, special attention is also given to other factors such as food matrix, food processing methods, and host factors on the absorption of seaweed polyphenols. In digestion tract, proteins can form both covalent and non-covalent bonds with polyphenols and thus hinder the absorption of polyphenols. However, lipids and polysaccharides in food can enhance the bioaccessibility of polyphenols to some extent. It is worth noticing that food processing technologies and host gut flora composition can also alter the absorption and bioactivity of specific seaweed phenolics. [ABSTRACT FROM AUTHOR]

Gaziantep (in Turkey) is one of the oldest cities in the World, in which the history of winemaking and viticulture began in ancient ages. It is home to wild white grapes Vitis vinifera Dökülgen and Paf. Three young white wines were produced from these two grapes. Ten phenolics and some chemical characteristics were quantitatively detected in the white wines and musts depending on white wine processes. Phenolic compounds significantly (p<0.05) increased during the fermentation process. After aging, (+)- catechin and procyanidin B2 contents of white wines were ranged from 1.12 to 1.35 and from 60.69 to 69.02 mg/L respectively. After aging, the quercetin, rutin and myricetin contents of white wines-1, 2 and 3 were ranged from 0.47 to 0.59, from 0.28 to 0.32 and from 0.11 to 13 mg/L respectively. White wines represented with abundant flavanols, tyrosol and chlorogenic acid produced from Dökülgen and Paf mixture with 7:3 ration. It was found that there is a significant difference between hydroxycinnamic acid and hydroxybenzoic acid content of white wines. Dökülgen and Paf white grapes contribute higher amount of phenolic characteristics, better acidity and sugar to white wine while Dökülgen grape contributed more sugar. [ABSTRACT FROM AUTHOR]

Hypertension (HTN) is one of the leading causes of death in the world. Agri-food by-products are emerging as a novel source of natural antihypertensive agents allowing for their valorization and making food and agricultural industries more environmentally friendly. In this regard, wine making process generates large amounts of by-products rich in phenolic compounds that have shown potential to exert several beneficial effects including antihypertensive properties. The aim of this study was to review the blood pressure-lowering effects of winery by-products. In addition, molecular mechanisms involved in their bioactivity were also evaluated. Among the winery by-products, grape seed extracts have widely shown antihypertensive properties in both animal and human studies. Moreover, recent evidence suggests that grape stem, skin and pomace and wine lees may also have great potential to manage HTN, although more studies are needed in order to confirm their potential in humans. Improvement of endothelial dysfunction and reduction of oxidative stress associated with HTN are the main mechanisms involved in the blood pressure-lowering effects of these by-products. [ABSTRACT FROM AUTHOR]

Phenolic compounds are one of the main organic pollutants in the environment that can seriously affect ecosystems, even at very low concentrations. Due to the resistance of phenolic compounds to microorganisms, conventional biological treatment methods face challenges in effectively addressing this pollution problem. In this study, a novel laccase mimic (Tris-Cu nanozyme) is prepared using a simple and rapid synthesis strategy based on the coordination of copper ions and amino groups in Tris(hydroxymethyl)aminomethane (Tris). It is found that the Tris-Cu nanozyme exhibits good catalytic activity against a variety of phenolic compounds, the Km, Vmax and Kcat are determined to be 0.18 mM, 15.62 μM·min−1 and 1.57 × 107 min−1 using 2,4-dichlorophenol (2,4-DP) as the substrate, respectively. Then, based on the laccase-like activity of the Tris-Cu nanozyme, a novel colorimetric method for 2,4-DP (the limit of detection (LOD) = 2.4 μM, S/N = 3) detection in the range of 10–400 μM was established, and its accuracy was verified by analyzing tap and lake water samples. In addition, the Tris-Cu nanozyme shows excellent removal abilities for six phenolic compounds in experiments. The removal percentages for 2,4-DP, 2-chlorophenol (2-CP), phenol, resorcinol, 2,6-dimethoxyphenol (2,6-DOP), and bisphenol A (BPA) are 100%, 100%, 100%, 100%, 87%, and 81% at 1 h, respectively. In the simulated effluent, the Tris-Cu nanozyme maintains its efficient catalytic activity towards 2,4-DP, with a degradation percentage of 76.36% at 7 min and a reaction rate constant (k0) of 0.2304 min−1. Therefore, this metal–organic complex shows promise for applications in the monitoring and degrading of environmental pollutants. [ABSTRACT FROM AUTHOR]

A mangrove plant known as Rhizophora apiculata is employed by Malay for treating skin diseases, diarrhea, vomiting, and nausea, as an antiseptic, for tanning, and also as fuelwood and fodder. Its large-scale use can be attributed to its high-quality timber, availability as well and the presence of a chemical named tannin that is employed for reinforcing fishing lines, nets, and ropes. The tannin content of R. apiculata's roots, bark, and leaves is regarded to be a natural inhibitor of fungal infections. This study is focused on determining the different kinds of functional groups, as well as individual phenolic compounds present in R. apiculata for identifying new bioactive compounds via decoding of the traditional values of Malay remedies. There is a high demand for such natural bioactive compounds, particularly in the healthcare and pharmaceutical markets. Alkaline fractional extracts were employed to design an analytical extraction method for R. apiculata. As per the HPLC results, there were three phenolic acids detected namely Caffeic acid, 4-Hydroxybenzoic acid, and Vanillic acid. Meanwhile, ten volatile compounds were identified by the GCTOF-MS. With regards to antibacterial activity, S. aureus, S. epidermidis, and E. coli were inhibited by R. apiculata leaf extract, while C. albicans and Fusarium sp. were inhibited by their antifungal activity. [ABSTRACT FROM AUTHOR]

The Abelmoschus esculentus plant, better known as okra, is an interesting crop from a nutritional standpoint. The okra plant is native to the African region but can now be found throughout tropical and subtropical areas of the world. This plant, known for its healing abilities, has been used as a traditional medicine to treat several diseases and external ailments, such as wounds or boils. This article reviews the potential health benefits from okra consumption, as well as the bioactive compounds that are suggested to be responsible. Furthermore, the okra plant and its derivatives have been evaluated in the formulation and manufacture of new functional food products. The latest advances in this direction, which includes characterizing the technical properties of functional foods fortified with okra are also presented in this review. A series of bioactive compounds such as flavonoids and catechins have been found in the okra plant, which were associated with numerous biological properties observed in research studies that reported potential anti-diabetic, anti-cancer, anti-hypertensive, and antimicrobial effects, among others, as a result of their consumption. These potential health benefits contribute to the development of new and useful functional foods, with okra (or its derivatives) being used as the highlighted ingredient. [ABSTRACT FROM AUTHOR]

Fruit by-products, due to their unique chemical composition containing dietary fibers and bioactive compounds, may favor the growth of probiotic strains. This study evaluated the fermentation of araticum, baru, and pequi by-products using Lactobacillus acidophilus (La-5, LA3, and NCFM) and Bifidobacterium animalis subsp. lactis (Bb-12) probiotic strains. We assessed probiotic viability, short-chain fatty acid levels, and bioactive compound levels after 48 h of fermentation. Araticum and pequi by-products led to counts higher than 6 log CFU/mL after 48-h fermentation for all Lactobacillus strains, but only the araticum by-product supported the growth of the Bb-12 strain. Fermentation of araticum by-product resulted in greater amounts of acetate (39.97 mM for LA3 and 39.08 mM for NCFM) and propionate (0.20 mM for NCFM), while baru by-product showed greater amounts of butyrate (0.20 mM for La-5 and Bb-12). Fermentation of araticum and baru by-products resulted in an increase in bioactive compounds, with the latter showing total phenolic compounds and antioxidant activity from 1.4 to 1.7 and from 1.3 to 3.1 times higher, respectively, than the negative control treatment. Araticum by-product exhibited a higher potential for prebiotic effects, and fermentation by the tested probiotic strains is essential to increase bioactive compound levels. [ABSTRACT FROM AUTHOR]

Antibiotic resistance reported in Pseudomonas sp. is associated with various opportunistic infections and is a concern in the public health system. The present study investigated the inhibitory effects of ethanol extracts of Lonicera japonica and Andrographis paniculata on Pseudomonas sp. The total phenolic compound (TPC) calculated in gallic acid equivalent (GAE) was 508.6±117.96 mg GAE/100 g dried weight (DW) in L. japonica while A. paniculata contained 129.0 ± 21.00 mg GAE/100 g DW. Both herbs significantly reduced the swarming zone diameters of Pseudomonas sp., even at a concentration as low as 5 mg/mL when compared to the controls. The anti-swarming effect of L. japonica and A. paniculata may be one of the mutual mechanisms underlying their antimicrobial properties. However, only the extract of L. japonica showed cell anti-adhesion activity, which can prevent biofilm formation. Andrographis paniculata extract on the other hand shows the highest activity in disrupting the cell membrane of Pseudomonas sp. [ABSTRACT FROM AUTHOR]

Beans (Phaseolus vulgaris), also known as the common beans, are broadly cultivated in temperate and semitropical regions. P. vulgaris contain different varieties, such as kidney beans, black beans, navy beans, pinto beans, and Great Northern beans. This legume is an excellent source of nutrients, including protein, carbohydrates, vitamins, minerals, and phytochemical compounds. The phytochemicals in beans comprise bioactive substances such as phenolic compounds, phytosterols, and oligosaccharides. These bioactive substances exhibit health and therapeutic benefits including antioxidant, cholesterol-lowering, anti-cancer, anti-diabetic, and anti-inflammatory potential. However, there is another category of essential phytochemicals, the antinutrients (e.g. enzyme inhibitors, phytic acids/phytates, saponins, and lectins), that negatively influence nutrient digestion and absorption. This review aims to describe the nutritional and phytochemical compounds of beans as well as the impact of different processing methods on bean phytochemicals, including soaking, extrusion, germination, roasting, steaming, autoclaving, boiling, cooking, microwaving, high-pressure processing, ultrasound, and pulsed electric field. In addition, the differences regarding volatile compounds; the impacts of storage on phytochemicals in beans; their bioaccessibility and digestibility; and the potential health benefits of bean consumption are introduced and evaluated. [ABSTRACT FROM AUTHOR]

Perilla frutescens is a medicinal herb that is commonly cultivated in Asian countries. Perilla seed is extensively pressed for cooking oil extraction. However, phenolic chemicals are still abundant in pressed perilla seed meal (PSM), which was previously thought to be useless after oil extraction. In our study, PSM was extracted using five solvents (water and 25%, 50%, 75%, and 100% ethanol) based on different ethanol concentrations, and its antioxidant activity, phenolic compounds, and inhibitory effects against key enzymes related to diabetes mellitus were evaluated. The 75% ethanol extract had higher phenolic (105.58 mg GAE/g DW) and flavonoid (66.52 mg QE/g DW) contents and showed better antioxidant and inhibitory effects against α‐glucosidase and α‐amylase. Analysis of the phenolic compounds of the five extracts by HPLC indicated the presence of apigenin, rosmarinic acid, benzoic acid, caffeic acid, and vanillic acid. Therefore, because of its high antioxidant activity and inhibitory capacity against enzymes relevant to diabetes, the 75% ethanol extract of perilla seed meal has the most potential to be used as a functional or nutraceutical food in the prevention and treatment of oxidation and diabetes. [ABSTRACT FROM AUTHOR]

Research on the composition of phenolic compounds in extracts of leaf blades of cloudberry (Rubus chamaemorus L.) populations from Lonno, Yelnya, Boloto Mokh, and Zhada reserves was conducted. The highest content of phenolic compounds was observed in the female clone of the Lonno population and amounted to 651.54 ± 21.64 mg-equiv of gallic acid per g of extract, of which 53.5 ± 5.01 mg-equiv of rutin per g of flavonoid extract. The following phenolic compounds were identified in the extracts using chromatography–mass-spectrometry: quercetin-3-O-xyloside, ellagic acid, quercetin-3-O-glucoside, quercetin-3-O-glucuronide, kaempferol-3-β-D-glucopyranoside, chlorogenic acid, and rutin. The contents of kaempferol-3-β-D-glucopyranoside, quercetin-3-O-glucoside, ellagic acid, and rutin were determined. [ABSTRACT FROM AUTHOR]

Legumes are a rich source of bioactive compounds such as phenolic or polyphenolic compounds, particularly tocopherols. Legumes antioxidants are widespread for their radical scavenging proprieties as active biologic compounds belonging to various chemical classes. Polyphenols are the most studied molecules of both nutritional and pharmaceutical interest. Furthermore, an overview concerning the antioxidant capacity and determination is mandatory for the precise and accurate method selection, involving cost-effectiveness and time-saving, towards gathering networks between research and development fields. The current review aims to summarize the presence of the natural antioxidant, their multiple biological effects, and the various approaches to methods determinations. [ABSTRACT FROM AUTHOR]

Citrus rootstocks are a relevant tree part that contributes to crops adapting to biotic and abiotic conditions, it becoming a key factor to face the current era of climate change. Although the emphasis of most studies on rootstocks has been placed on the yield and optimization of the citrus fruit grown in different environments, other studies have paid attention to the effect of rootstock on fruit quality. Hence, the quality of citrus fruit is becoming increasingly more relevant as consumers demand high internal and external qualities to eat citrus fruit fresh. To better understand how rootstock influences citrus fruit quality, the literature that focuses on physico-chemical parameters, nutritional compounds and physiological disorders has been revised. This review points out the influence that the external aspects surrounding plants has on fruit quality, such as the rootstock/scion interaction, the water uptake capacity of roots, the modification of the photosynthetic rate or availability of nutrient minerals. [ABSTRACT FROM AUTHOR]

Peanut sprout is a high-quality healthy food, which not only has beneficial effects, but also a higher phenol content than peanut seed. In this study, peanut sprout was treated with five cooking methods, namely boiling, steaming, microwave heating, roasting, and deep-frying, and the phenol content, monomeric phenol composition, and antioxidant activity were determined. The results showed that, compared with unripened peanut sprout, the total phenol content (TPC) and total flavonoid content (TFC) decreased significantly after the five ripening processes, and the highest retention of phenols and flavonoids was associated with microwave heating (82.05% for TPC; 85.35% for TFC). Compared with unripened peanut sprout, the monomeric phenol composition in germinated peanut was variable after heat processing. After microwave heating, except for a significant increase in the cinnamic acid content, no changes in the contents of resveratrol, ferulic acid, sinapic acid, and epicatechin were observed. Furthermore, there was a significant positive correlation of TPC and TFC with 2,2-diphenyl-1-picrylhydrazyl scavenging capacity, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging capacity, and ferric ion reducing antioxidant power in germinated peanut, but not with hydroxyl free radical scavenging capacity, in which the main monomer phenolic compounds were resveratrol, catechin, and quercetin. The research results indicate that microwave heating can effectively retain the phenolic substances and antioxidant activity in germinated peanuts, making it a more suitable ripening and processing method for germinated peanuts. [ABSTRACT FROM AUTHOR]

In the present study, we used exogenous naringenin (0.5 mM) pretreatment before the stress (25 mM NaCl) on the growth and tolerance of safflower seedlings under non-salinity conditions and salinity conditions. Our results showed that salinity treatment significantly declined the biomass, leaf relative water content, chlorophyll content, K+ content, and K+/Na+ ratio by 28%, 28%, 12%, 36%, and 56%, respectively, as compared to untreated control. The results obtained in the present study showed the beneficial effects of the pretreatment of naringenin in safflower seedlings under non-salinity conditions concerning increasing plant biomass, total phenolic compound, radical scavenging activity (RSA), soluble sugar content, proline, glutathione, enzymatic antioxidants, and K+ content. Nevertheless, naringenin pretreated plants showed a clear increment in the values of biomass, RSA, total phenolic compound, and catalase enzyme activity parameters under salinity stress. Salinity stress caused ionic phytotoxicity and oxidative stress by enhancing Na+ content, H2O2 accumulation, malondialdehyde (MDA), and antioxidants. However, naringenin alleviated salt-induced oxidative stress by decreasing H2O2 and MDA content in the leaves and improving the catalase activity in treated plants. Generally, it could be concluded pretreatment of naringenin before stress could partly diminish NaCl-caused oxidative stress in safflower seedlings, probably due to improvement in enzymatic and non-enzymatic antioxidants and reduced cell membrane damage. We report for the first time that applying exogenous naringenin pretreatment before the stress could improve growth and diminish NaCl-caused oxidative stress in safflower seedlings, probably due to the improvement in enzymatic and non-enzymatic antioxidants and reduced cell membrane damage. This implies that applying exogenous naringenin pretreatment before the stress is a promising approach for sustainable crop production under salinity stress. [ABSTRACT FROM AUTHOR]

The effects of pre- and postharvest calcium gluconate (Ca-Glu) treatments on some physicochemical characteristics and bioactive compounds of sweet cherry cv. Sweetheart during cold storage were investigated. For preharvest treatments, the Ca-Glu (1%) solution was applied to the cherry trees two times at 21 and 35 days after full bloom stage. Control trees were sprayed with distilled water at the same days. Sweet cherries, sprayed with and without Ca-Glu, were dipped into cold water (4 °C) containing calcium gluconate (1%) for 30 s and only in cold water (4 °C) as control, after harvest Following each treatment, cherries were placed in plastic boxes and stored at 1 ± 0.5 °C and 90 ± 5% relative humidity for 3 weeks. The weight losses of cherries increased over time but calcium (Ca) treatments, especially pre-and postharvest combination, limited these increases compared to control groups. The best result for suppressing the respiration rate of cherries was also obtained from combined treatment. Moreover, combined treatment delayed the losses of titratable acidity, fruit firmness, decay rate and sensory quality in sweet cherries during storage comparison with the pre or postharvest application of Ca-Glu alone. The effect of Ca-Clu treatments on stem chlorophyll content and antioxidant activity was not significant. Preharvest and combined treatments retarded the loss of ascorbic acid content of cherries compared to postharvest and control treatments. The total phenolic and anthocyanin content increased regularly throughout storage, regardless of treatment; however, Ca treatments delayed the accumulation of these compounds. As a result, the combined Ca-Glu treatment could be a promising method for maintaining some physicochemical characteristics and bioactive compounds in sweet cherries during cold storage. [ABSTRACT FROM AUTHOR]

The effects of gamma irradiation and pasteurization on the stability of anthocyanins and antioxidant capacity during storage of jussara pulp were investigated. Jussara pulp was divided into 6 portions: control (no treatment), irradiated pulp (2, 4, 6, 8 kGy) and pasteurized pulp (92 °C/1 minute). Portions were stored at 4°C for 60 days. The phenolic extract was prepared with a solution of methanol/water/formic acid. The following analyses were performed every 15 days: contents of total phenolic compounds by Folin-Ciocauteau, cyanidin-3-glycoside and cyanidin-3-rutiniside by HPLC and antioxidant capacity (ABTS and DPPH). Total phenolics and anthocyanins decreased with the increasing irradiation dose and storage time. Pasteurization did not affect the phenolic concentration immediately after processing. However, the contents of TPC and cyanidin-3-rutiniside were reduced during storage of the pasteurized pulp. No processed samples presented characteristics similar to the control at the end of storage. [ABSTRACT FROM AUTHOR]

Objective: Although there are scientific studies on the effectiveness of various products (syrup, molasses, extract, etc.) obtained from black mulberry (morus nigra) in the treatment of oral mucositis, the results are not yet sufficient to establish a standard. Black mulberry is not included in the metaanalysis yet. There is a need for randomized controlled studies and high-proof results regarding its effect. Therefore, the results obtained from the studies will be an important data source for the literature and consensus studies on OM. The aim of this study is to determine the syrup types obtained from Black Mulberry and the Total Phenolic Compound, Total Flavonoid and Total Antioxidant Activity Values of Lyophilized Black Mulberry (morus nigra), whose content has not been determined before and evaluated for the first time, and to explain their importance in oral mucositis care. Method: The total phenolic, total flavonoid and total antioxidant activities of Lyophilized Black Mulberry (morus nigra) and Black Mulberry Syrups that we will use in our study were evaluated spectrophotometrically. Results: Lyophilized Black Mulberry (morus nigra) 1; The total phenolic compound value was 3.23% mg/GAE, the total flavonoid amount was 0.58 mg/KE, and the total antioxidant activity value was 2.88% mg/TEAC. According to Lyophilized Black Mulberry (morus nigra) 1 purchased from a commercial company, Lyophilized Black Mulberry (morus nigra) 2 prepared in the laboratory, Black Mulberry Syrup 1, Black Mulberry Syrup 2 and Black Mulberry Syrup 3 with the highest market value in pharmacies, fresh Black Mulberry (morus nigra) is the most determined as a product with close content. Conclusion: It was determined that Lyophilized Black Mulberry (morus nigra) 1 and Lyophilized Black Mulberry (morus nigra) 2 had very good values in terms of total phenolic, total flavonoid and total antioxidant activities. In this respect, it is thought that lyophilized black mulberry (morus nigra) can be used in the treatment of effective oral mucositis in addition to its forms such as syrup, extract and molasses. [ABSTRACT FROM AUTHOR]

Advanced glycation end-products (AGEs) favor inflammation and oxidative stress, playing a role in chronic diseases pathogenesis. Grape polyphenols exert antiglycative and antioxidant effects which may contribute to prevent chronic diseases. However, clinical evidence of grape polyphenols on chronic disease prevention and treatment by glycation markers modulation are limited. Therefore, we aimed to critically analyze studies about that topic to investigate the antiglycative power of dietary grape polyphenol, and to explore the molecular mechanism involved. This systematic review was conducted and reported according to PRISMA guidelines. The following search terms were used: "grape", "extract", "grape seed extract", "grape skin extract", "polyphenol extract", "grape polyphenol(s)", "grape juice", "resveratrol", "quercetin", "catechin", "epicatechin", "procyanidin(s)", and "anthocyanin(s)". Seven studies were included. Glycated hemoglobin was not affected. The interventions duration may not have been enough to detect changes. Grape polyphenols reduced fructosamine and methylglyoxal (MGO) concentrations, and increased endogenous secretory RAGE (esRAGE) gene expression but did not affect the serum concentration. Resveratrol antiglycative effects are mainly due its ability to trap MGO and downregulate RAGE. In conclusion, grape polyphenols may have a positive impact on early glycation products, AGEs and esRAGE. Future studies are needed to explore how they modulate AGEs and receptors in chronic diseases. [ABSTRACT FROM AUTHOR]

Agaricus biosporus is a mushroom known for its use for nutritional and medicinal purposes. This study tested whether silver nanoparticles (AgNPs) synthesized from phenolic compounds extracted from this mushroom have an efficient antimicrobial impact against pathogenic bacteria and yeast isolated from urine, sputum, and vaginal swabs. Different methods were employed in the characterization of AgNPs. The microorganisms of isolated bacteria and yeast were identified according to morphological features, results of biochemical tests, and the VITEK 2 compact system. The susceptibility to antibiotics was determined by the disk method. The bacteria and yeast resisted all antibiotics employed in this research. But the AgNPs had good antimicrobial activity against pathogenic bacteria and yeast. The highest inhibition zone of AgNPs against Pseudomonas aeruginosa in a 100 mg/mL concentration was 30 mm, while the minimal inhibition zone of AgNPs was 12 mm against Escherichia coli at the same concentration. The antibacterial effect of the synthesized AgNPs was concentration-dependent against Gram-positive and Gram-negative bacteria. With respect to the effect on yeast growth, there was a clear inhibitory effect of the synthesized AgNPs at 100 mg/mL, when the largest zone of inhibition was found against Candida glabrata (29 mm), and the minimal inhibition zone was found against C. guillermondi and C. albican (23 mm). Similarly, the AgNPs synergized with antibiotics were used against pathogenic bacterial and yeast isolates because when antibiotics and AgNPs were combined, the inhibition zones were larger compared to those of the antibiotics alone. [ABSTRACT FROM AUTHOR]

Propolis is a complex natural compound that honeybees obtain from plants and contributes to hive safety. It is rich in phenolic and flavonoid compounds, which contain antioxidant, antimicrobial, and anticancer properties. In this study, the chemical composition and antioxidant activities of propolis were investigated; ABTS•+, DPPH• and DMPD•+ were prepared using radical scavenging antioxidant methods. The phenolic and flavonoid contents of propolis were 53 mg of gallic acid equivalent (GAE)/g and 170.164 mg of quercetin equivalent (QE)/g, respectively. The ferric ion (Fe3+) reduction, CUPRAC and FRAP reduction capacities were also studied. The antioxidant and reducing capacities of propolis were compared with those of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and Trolox reference standards. The half maximal inhibition concentration (IC50) values of propolis for ABTS•+, DPPH• and DMPD•+ scavenging activities were found to be 8.15, 20.55 and 86.64 μg/mL, respectively. Propolis extract demonstrated IC50 values of 3.7, 3.4 and 19.6 μg/mL against α-glycosidase, acetylcholinesterase (AChE) and carbonic anhydrase II (hCA II) enzyme, respectively. These enzymes' inhibition was associated with diabetes, Alzheimer's disease (AD) and glaucoma. The reducing power, antioxidant activity and enzyme inhibition capacity of propolis extract were comparable to those demonstrated by the standards. Twenty-eight phenolic compounds, including acacetin, caffeic acid, p-coumaric acid, naringenin, chrysin, quinic acid, quercetin, and ferulic acid, were determined by LC-MS/MS to be major organic compounds in propolis. The polyphenolic antioxidant-rich content of the ethanol extract of propolis appears to be a natural product that can be used in the treatment of diabetes, AD, glaucoma, epilepsy, and cancerous diseases. [ABSTRACT FROM AUTHOR]

The aim of this work was to quantify and identify the phenolic composition of Centaurea kilae Bosse and record the anti‐alzheimer, anti‐urease, anti‐lipase, antidiabetic, anti‐melanogenesis, and antibacterial properties. IC50 values for enzymes were obtained between 0.370–180.1 μg/mL and this plant inhibited HMG_CoA R and urease enzymes more with IC50 values of 0.370 and 39.09 μg/mL, respectively. Furthermore, molecular docking calculations were done subsequently, using targets, HMGCoA_R, glucosidase, amylase, and lipase to detect the structural necessity in activity for the phenolic compounds of Centaurea kilae plant. Chemical compounds of methanolic extracts of Centaurea kilae determined in this work. Finally, antibacterial and antifungal effects of this plant investigated. [ABSTRACT FROM AUTHOR]

In this study, optimization of the extraction of phenol compounds from Mentha piperita using ultrasonic waves with response surface methodology (RSM) was assessed. In this regard, a central composite design with three independent variables of time (5, 27.5, and 50 min), temperature (25, 45, and 65°C), and concentrations of ethanol in the water–ethanol solution (0%, 50%, and 100%) was used. Besides, the antioxidant activity tests (DPPH radical scavenging assay, ferric reducing antioxidant power [FRAP], and oxidative stability indexes [OSI]) were examined. Significant effects of independent variables on the extraction of phenol compound, DPPH radical scavenging power, and OSI of M. piperita extract, with the regression coefficients of 0.89, 0.92, and 0.94, respectively, were noted. However, no significant difference in terms of the FRAP among different treatments was noted. Also, the best antioxidant activity of M. piperita was obtained by using the ultrasonic wave for 50 min at 65°C and 59.6% v/v ethanol/water solution. While the findings of RSM confirmed the experimental results, due to the favored properties of M. piperita extract by the proposed method, further research to investigate possible applications in the food industry is recommended. [ABSTRACT FROM AUTHOR]

It has been demonstrated that lignin was efficiently degraded by Lentinula edodes (L. edodes). However, the process of lignin degradation and utilization by L. edodes has not been discussed in detail. Therefore, the effects of lignin on L. edodes mycelium growth, chemical compositions, and phenolic profiles were investigated herein. It has been revealed that 0.10% lignin acted as the most effective concentration to accelerate mycelia growth, which yielded the highest biomass of 5.32 ± 0.07 g/L. Furthermore, a 0.10% concentration of lignin promoted the accumulation of phenolic compounds, especially protocatechuic acid, with peak value of 48.5 ± 1.2 μg/g. In contrast, the higher concentration of lignin (0.20%) exerted an inhibitory effect on the growth of L. edodes. Overall, the application of lignin at the optimal concentration of 0.10% could not only enhance the mycelial growth but also accumulate the phenolic acids and raise the nutritional and medical values of L. edodes. [ABSTRACT FROM AUTHOR]

Extra virgin olive oils (EVOOs) obtained from five Turkish olive cultivars widely produced in the Aegean and Marmara regions were investigated based on their total antioxidant capacity (TAC), total phenolic content (TPC), pigment contents, fatty acid (FA) profiles, phenolic compounds (PC), volatile compounds (VC), and sensory properties. The results showed that all properties of EVOO samples were significantly affected by the olive cultivar used. The pigment contents in Ayvalık (9.90 mg·kg−1) and Uslu (9.00 mg·kg−1) oils were higher than the others (p < 0.05). The greatest values for oleic acid (74.13%) and TPC (350.6 mg·kg−1) were observed in Gemlik and Domat oils, respectively (p < 0.05). Edincik oil showed the maximum hydroxytyrosol content (48.022 mg·kg−1) and TAC value (515.36 mg TE·kg−1) (p < 0.05). The Edincik, Domat, and Uslu oils were significantly not different for the total content of C6 compounds derived by lipoxygenase, which are the main volatiles responsible for the typical aroma of EVOOs (p > 0.05). Domat oil also exhibited the highest scores for bitterness and pungency perceptions (p < 0.05). The fruitiness scores of the oil samples (except for Ayvalık oil) were close to each other, even if they were statistically different (p < 0.05). Principal component analysis (PCA) indicated that the Ayvalık oil was separated from the others due to its poor-quality characteristics. As a result, it can be stated that Domat olive oil has better quality than the others. [ABSTRACT FROM AUTHOR]

BACKGROUND: Food processing produces large volumes of waste that can be transformed into useful and relevant ingredients. The by‐product of oil extraction from faveleira seeds is a potentially low‐cost raw material that can be used for obtaining functional foods. This study aimed to analyze the physicochemical properties, chemical composition, and antioxidant activity of faveleira (Cnidoscolus quercifolius Pohl) seed press cake flour (FSPCF). Additionally, the chemical composition, antioxidant activity, and physical, microbiological, and sensory aspects of cookies with 0, 25%, or 50% substitution of refined wheat flour (RWF) with FSPCF (0‐FSPCF, 25‐FSPCF, and 50‐FSPCF cookies, respectively) were evaluated. RESULTS: FSPCF exhibited good physicochemical properties, high antioxidant activity (0.45 ± 0.00 and 42.83 ± 1.30 g TE g−1 for the DPPH and ABTS methods, respectively), and high polyphenol content (particularly gallic acid at 21015.85 ± 4981.76 g kg−1) and is also rich in minerals and fiber (359.40 ± 1.10 g kg−1). Replacement of RWF with FSPCF increased the activity of antioxidants and the levels of polyphenols, ash, lipids, proteins, and fibers. The 50‐FSPCF cookie possessed the highest linoleic acid content (97.50 ± 8.47 g kg−1). Flour replacement influenced the weight of the cookies without affecting the other physical characteristics. The cookies yielded good sensory acceptance and purchase intentions. Contamination was not detected. CONCLUSION: Faveleira flour possesses high nutritional and bioactive value and can be used as a functional ingredient in cookies and possibly in other bakery products such as bread, cakes, and pastas. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Paulownia spp. is a genus of trees in the Paulowniaceae family. It is native to southeastern Asia (especially China), where it has been cultivated for decorative, cultural, and medicinal purposes for over 2000 years. Depending on taxonomic classification, there are 6 to 17 species of Paulownia; P. tomentosa, P. elongata, P. fortunei, and P. catalpifolia are considered the most popular. Nowadays, Paulownia trees are planted in Asia, Europe, North America, and Australia for commercial, medical, and decorative purposes. Lately, growing interest in Paulownia has led to the development of various hybrids, the best-known being Clone in vitro 112, Shan Tong, Sundsu 11, and Cotevisa 2. Paulownia Clone in vitro 112 is an artificially created hybrid of two species of Paulownia: P. elongata and P. fortunei. The present review of selected papers from electronic databases including PubMed, ScienceDirect, and SCOPUS before 15 November 2022 describes the phytochemical characteristics, biological properties, and economic significance of various organs from different Paulownia species and hybrids, including P. tomentosa, P. elongata, P. fortunei, and Paulownia Clone in vitro 112. Many compounds from Paulownia demonstrate various biological activities and are promising candidates for natural preparations; for example, the leaves of Clone in vitro 112 have anti-radical and anticoagulant potential. However, further in vivo studies are needed to clarify the exact mechanism of action of the active substances and their long-term effects. [ABSTRACT FROM AUTHOR]

In this study, Iranian Allium sativum controversum extracts, as a valuable source of bioactive compounds such as antioxidants, extracted by solvents were analyzed. Based on the analysis of total phenolic content (TPC) and total flavonoid content (TFC) and radical scavenging activity (1,1‐diphenyl‐2‐picrylhydrazyl (DPPH)) of each extract, ethanol extracts were finally added to the sausage formulation at 0.5 and 1.5%w/v. Treatments were kept at refrigerated temperature (4–5°C) for 1, 15, and 30 days, and DPPH and microbial assays were performed on the treatments and the control samples. Experimental data were performed in a completely randomized design with the factorial arrangement. Hydroalcoholic extract had the highest total phenols and the aqueous extract of Allium sativum controversum showed the highest radical scavenging activity (11.85 ± 0.81 mg/g). No colony counts were observed on the first day of the coliform count. On 15 to 30 days, the control sample showed the highest count and the treatment containing Allium sativum controversum extract (1.5%) had the lowest coliform count. During the first month, the control sample had the highest count of Staphylococcus aureus. Regarding mold and yeast, a treatment containing Allium sativum controversum extract (1.5%) and the control sample had the lowest and highest count, respectively. The results showed that using Allium sativum controversum extracts and increased radical scavenging activity reduced microbial growth during the storage period. [ABSTRACT FROM AUTHOR]

The aims of the present study were to evaluate the protective effects of gallic acid against doxorubicin-induced ovarian toxicity in mice, and to verify the possible involvement of PI3K and mTOR signaling pathway members (PTEN, Akt, FOXO3a and rpS6) in the gallic acid protective actions. Mice were pretreated with NaCl (0.15 M, p.o.) (control and doxorubicin groups) or gallic acid (50, 100 or 200 mg/kg body weight, p.o.) once daily, for 5 days, and on the third day of treatment, after 1 h of treatment administration, the mice received saline solution (i.p.) (control group) or doxorubicin (10 mg/kg of body weight, i.p.). Next, the ovaries were harvested for histological (follicular morphology and activation), fluorescence (GSH and mitochondrial activity), and immunohistochemical (PCNA, cleaved caspase-3, TNF-α, p-PTEN, Akt, p-Akt, p-rpS6 and p-FOXO3a) analyses. The results showed that cotreatment with 50 mg/kg gallic acid plus doxorubicin preserved the percentage of normal follicles and cell proliferation, reduced the percentage of cleaved caspase-3 follicles, prevented inflammation, and increased GSH concentrations and mitochondrial activity compared to doxorubicin treatment alone. Furthermore, cotreatment 50 mg/kg gallic acid plus doxorrubicin increased expression of Akt, p-Akt, p-rpS6 and p-FOXO3a compared to the doxorubicin alone. In conclusion, 50 mg/kg gallic acid protects the mouse ovary against doxorubicin-induced damage by improving GSH concentrations and mitochondrial activity and cellular proliferation, inhibiting inflammation and apoptosis, and regulating PI3K and mTOR signaling pathway. • Gallic acid increases survival and inhibits inflammation in ovaries treated with doxorubicin. • Doxorubicin reduces antioxidant mechanisms and increased TNF-α and apoptosis. • Gallic acid regulates the PI3K and mTOR pathway in doxorubicin-treated ovaries. [ABSTRACT FROM AUTHOR]

Phenolic compounds extracted from walnut shells are potentially good natural sources of antioxidants for the food industry and have numerous health benefits. Walnuts have more antioxidant capacity than any other nut because the shell is primarily composed of lignin, a strong source of phenols. Studies demonstrated that lignin characterizes the shell strength level and is a source of antioxidants due to its chemical composition. In the current study, an extract obtained by extraction with a hydroalcoholic solvent of various concentrations from a walnut shell was investigated. The results of this study have proven that walnut shell extract contains the main sources of mineral elements and vitamins, which are of great importance. According to the biological value, this extract contains essential amino acids for the body. The high content of quercetin and catechin shows the antioxidant activity of the extract. In the present article, the authors disclose methods for obtaining an experimental batch of a prophylactic product based on walnut shells and give the product a technological characteristic. Consequently, a product was developed for prophylactic usage of 10 ml per 100 ml of water and must be taken 1-2 times a day for 21 days. The required product amount was calculated from the daily intake of vitamins, minerals, and flavonoids. [ABSTRACT FROM AUTHOR]