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Two new species of the genus Trispinaria Quicke, 1986, from Vietnam, viz. T. seminigra Long, sp. nov. and T. vietnamica Long, sp. nov., are described and fully illustrated. Additionally, this is the first record of the genus Trispinaria in Vietnam. A checklist with distributions of previously described species of the genus Trispinaria is given. Comparative characters of the Vietnamese species are provided and modified key couplets are provided to facilitate their identification.

While CD4 Th1 cells are required for resistance to intramacrophage infections, adoptive transfer of Th1 cells is insufficient to protect against Salmonella infection. Using an epitope-tagged vaccine strain of Salmonella, we found that effective protection correlated with expanded Salmonella-specific memory CD4 T cells in circulation and nonlymphoid tissues. However, naive mice that previously shared a blood supply with vaccinated partners lacked T cell memory with characteristics of tissue residence and did not acquire robust protective immunity. Using a YFP-IFN-γ reporter system, we identified Th1 cells in the liver of immunized mice that displayed markers of tissue residence, including P2X7, ARTC2, LFA-1, and CD101. Adoptive transfer of liver memory cells after ARTC2 blockade increased protection against highly virulent bacteria. Taken together, these data demonstrate that noncirculating memory Th1 cells are a vital component of immunity to Salmonella infection and should be the focus of vaccine strategies.

The development of a subunit Salmonella vaccine has been hindered by the absence of detailed information about antigenic targets of protective Salmonella-specific T and B cells. Recent studies have identified SseB as a modestly protective Ag in susceptible C57BL/6 mice, but the mechanism of protective immunity remains undefined. In this article, we report that simply combining Salmonella SseB with flagellin substantially enhances protective immunity, allowing immunized C57BL/6 mice to survive for up to 30 d following challenge with virulent bacteria. Surprisingly, the enhancing effect of flagellin did not require flagellin Ag targeting during secondary responses or recognition of flagellin by TLR5. Although coimmunization with flagellin did not affect SseB-specific Ab responses, it modestly boosted CD4 responses. In addition, protective immunity was effectively transferred in circulation to parabionts of immunized mice, demonstrating that tissue-resident memory is not required for vaccine-induced protection. Finally, protective immunity required host expression of IFN-γR but was independent of induced NO synthase expression. Taken together, these data indicate that Salmonella flagellin has unique adjuvant properties that improve SseB-mediated protective immunity provided by circulating memory.

This is the most complete list of Aleiodes species of the subfamily Rogadinae from Vietnam, and of the total seventy five species of this genus reported, sixty three species of the subgenus Aleiodes, five of Arcaleiodes, three of Chelonorhogas, and four species of the genus Heterogamus. There are thirty species newly recorded from the Rogadinae fauna of Vietnam. The information on valid names, the published papers of the first record of Aleiodes species in Vietnam and the distribution of Aleiodes species were also provided. Additionally, there are three species listed with information about their hosts, being agricultural pests controlled to some degree by these parasitoids. [ABSTRACT FROM AUTHOR]

It has been demonstrated that the two main subsets of peripheral T cells, V1 and V4, have divergent functions in many diseases models. Recently, we reported that V4 T cells played a protective role in tumor immunity through eomesodermin-controlled mechanisms. However, the precise roles of V1 T cells in tumor immunity, especially whether V1 T cells have any interaction with V4 T cells, remain unknown. We demonstrated in this paper that V1 T cells suppressed V4 T cell-mediated antitumor function both in vitro and in vivo, and this suppression was cell contact independent. Using neutralizing anti-IL-4 Ab or IL-4-/- mice, we determined the suppressive factor derived from V1 T cells was IL-4. Indeed, treatment of V4 T cells with rIL-4 significantly reduced expression levels of NKG2D, perforin, and IFN-. Finally, V1 T cells produced more IL-4 and expressed significantly higher level of GATA-3 upon Th2 priming in comparison with V4 T cells. Therefore, to our knowledge, our results established for the first time a negative regulatory role of V1 T cells in V4 T cell-mediated antitumor immunity through cell contact-independent and IL-4-mediated mechanisms. Selective depletion of this suppressive subset of T cells may be beneficial for tumor immune therapy. [ABSTRACT FROM AUTHOR]

In response to infection, CD8+ T cells integrate multiple signals and undergo an exponential increase in cell numbers. Simultaneously, a dynamic differentiation process occurs, resulting in the formation of short-lived effector cells (SLECs; CD127lowKLRG1high) and memory precursor effector cells (CD127highKLRG1low) from an early effector cell that is CD127lowKLRG1low in phenotype. CD8+ T cell differentiation during vesicular stomatitis virus infection differed significantly than during Listeria monocytogenes infection with a substantial reduction in early effector cell differentiation into SLECs. SLEC generation was dependent on Ebi3 expression. Furthermore, SLEC differentiation during vesicular stomatitis virus infection was enhanced by administration of CpG-DNA, through an IL-12-dependent mechanism. Moreover, CpG-DNA treatment enhanced effector CD8+ T cell functionality and memory subset distribution, but in an IL-12-independent manner. Population dynamics were dramatically different during secondary CD8+ T cell responses, with a much greater accumulation of SLECs and the appearance of a significant number of CD127highKLRG1high memory cells, both of which were intrinsic to the memory CD8+ T cell. These subsets persisted for several months but were less effective in recall than memory precursor effector cells. Thus, our data shed light on how varying the context of T cell priming alters downstream effector and memory CD8+ T cell differentiation. [ABSTRACT FROM AUTHOR]