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Previously, two men were cured of HIV-1 through CCR5Δ32 homozygous (CCR5Δ32/Δ32) allogeneic adult stem cell transplant. We report the first remission and possible HIV-1 cure in a mixed-race woman who received a CCR5Δ32/Δ32 haplo-cord transplant (cord blood cells combined with haploidentical stem cells from an adult) to treat acute myeloid leukemia (AML). Peripheral blood chimerism was 100% CCR5Δ32/Δ32 cord blood by week 14 post-transplant and persisted through 4.8 years of follow-up. Immune reconstitution was associated with (1) loss of detectable replication-competent HIV-1 reservoirs, (2) loss of HIV-1-specific immune responses, (3) in vitro resistance to X4 and R5 laboratory variants, including pre-transplant autologous latent reservoir isolates, and (4) 18 months of HIV-1 control with aviremia, off antiretroviral therapy, starting at 37 months post-transplant. CCR5Δ32/Δ32 haplo-cord transplant achieved remission and a possible HIV-1 cure for a person of diverse ancestry, living with HIV-1, who required a stem cell transplant for acute leukemia.

ABSTRACTThe UN’s Zero Tolerance Policy, which bans all relationships between UN staff and locals, portrays all relationships as exploitative, fails to account for nuances in these relationships and does not acknowledge the agency of local women or communities. This study uses community-based qualitative data from eastern DRC that shares narratives on a wide variety of consensual relationships between peacekeepers and local women. Our paper uses a data-driven approach, including a post-colonial feminist lens, and ideas of structural agency to provide an expanded definition of agency that invites readers to re-examine their views of women in conflict settings. Finally, we provide clear recommendations for the UN and other international non-governmental agencies on policies related to sexual exploitation and abuse.

Objective We aimed to determine the association of statins with progression to a high risk for advanced fibrosis in primary care patients with metabolic dysfunction-associated steatotic liver disease (MASLD).Design This retrospective cohort study of electronic health record data included patients with MASLD and an initial low or indeterminate risk for advanced fibrosis, determined by Fibrosis-4 Index (FIB-4) score (

Background: The management of locally advanced rectal cancer (LARC) relies on a multimodal approach. Neither instrumental work-up nor molecular biomarkers are currently available to identify a risk-adapted strategy. Objectives: We aim to investigate the role of circulating tumor DNA (ctDNA) and its clearance at different timepoints during chemo-radiotherapy (CRT) and correlate them with clinical outcomes. Design: Between November 2014 and November 2019, we conducted a monocentric prospective observational study enrolling consecutive patients with LARC managed with neoadjuvant standard CRT (capecitabine and concomitant pelvic long-course radiotherapy), followed by consolidation capecitabine in selected cases and surgery. Methods: Blood samples for ctDNA were obtained at pre-planned timepoints. We evaluated the correlation of baseline variant allele frequency (VAF) with pathologic complete response (pCR) down-staging, node regression (pN0), event-free survival (EFS), and overall survival (OS). Results: Among 112 screened patients, 61 were enrolled. In all, 38 (62%) had a positive ctDNA at baseline with VAF > 0 and 23 had negative ctDNA (VAF = 0). Among patients with negative ctDNA, 30% had a complete response, while only 13% of positive ctDNA patients had pCR [odds ratio (OR) 0.35 (95% confidence interval (CI): 0.10–1.26), p = 0.11]. Similarly, 96% and 74% of pN0 were observed among negative and positive ctDNA patients, respectively [OR 0.13 (95% CI: 0.02–1.07), p = 0.058]. The presence of a baseline VAF > 0 was associated with a trend toward a lower EFS compared with VAF = 0 patients [hazard ratio (HR) = 2.30, 95% CI: 0.63–8.36, p = 0.21]. Within the limitations of small sample size, no difference in OS was observed according to the baseline ctDNA status (HR = 1.18, 95% CI: 0.35–4.06, p = 0.79). Conclusion: Within the limitations of a reduced number of patients, patients with baseline negative ctDNA seem to show a higher probability of pN0 status and a trend toward improved EFS. Prospective translational studies are required to define the role of ctDNA analysis in the multimodal treatment of LARC.

This study evaluated the significance and predictive value of ultrasonographic and physical examination on arrival at an Austrian fattening farm. Treatment frequency and average daily weight gain (ADG) were related to physical and ultrasonographic examination results. Additionally, the effect of an intranasal vaccination in half of the examined calves was studied. The clinical and ultrasonographic health status 600 calves was recorded at the beginning and end of fattening. Half of the calves received an intranasal vaccination (Bovalto® Respi Intranasal). Overall, 44.5% showed an abnormal respiratory scoring (RS) and 56.0% showed signs of respiratory diseases in transthoracic ultrasonography (TUS) at arrival on the farm. For both RS and TUS, a categorization between ILL and HEALTHY was conducted. Results showed lower ADG in ILL calves (RS median: 0.93 kg/d; TUS median: 0.96 kg/d) compared to HEALTHY calves (RS median: 1.01 kg/d; TUS median: 1.01 kg/d). The median ADG was lower in not treated and ILL calves (RS median 0.90 kg/d; TUS: 0.93 kg/d) compared to treated and ILL calves (RS median 1.01 kg/d; TUS: 1.02 kg/d). Vaccination did not affect growth performance or occurrence of ILL, though treatment frequency was lower in VAC calves (17.0% in NVAC; 11.3% in VAC). The implementation of examination protocols for respiratory diseases may have a positive impact on production parameters (e.g., treatment frequency and ADG).

Abstract Background Chewing is an essential physiological process in horses; yet, the physical form of feeds may affect their chewing and feeding behaviour with consequences for equine digestion and health. Objectives and methods The present study evaluated the potential of a commercial forage cubes made from alfalfa and mixed meadow grasses to maintain chewing activity when compared with a traditional long and fibre‐rich hay. An additional aim was to measure the dust formation during feeding. The experiment was a crossover design with six horses (11 ± 4 years old, mean ± SD), fed with 5 kg (as‐fed basis) of their feed (long hay or alfalfa cubes) overnight. Eating and chewing activity was measured using the EquiWatch system with a sensor‐based halter. Results Data showed that by feeding the same amount of feed overnight, cube feeding led to 24% shorter eating time (on average 67 min less) and 26% fewer total chews compared to the long hay. On an hourly basis, horses also spent more time eating and chewing the long hay compared to the cubes. The cube feeding led to a higher dust concentration of the inhalable fraction (

Abstract Background The association between microbes and cancer has been reported repeatedly; however, it is not clear if molecular tumour properties are connected to specific microbial colonisation patterns. This is due mainly to the current technical and analytical strategy limitations to characterise tumour-associated bacteria. Methods Here, we propose an approach to detect bacterial signals in human RNA sequencing data and associate them with the clinical and molecular properties of the tumours. The method was tested on public datasets from The Cancer Genome Atlas, and its accuracy was assessed on a new cohort of colorectal cancer patients. Results Our analysis shows that intratumoural microbiome composition is correlated with survival, anatomic location, microsatellite instability, consensus molecular subtype and immune cell infiltration in colon tumours. In particular, we find Faecalibacterium prausnitzii, Coprococcus comes, Bacteroides spp., Fusobacterium spp. and Clostridium spp. to be strongly associated with tumour properties. Conclusions We implemented an approach to concurrently analyse clinical and molecular properties of the tumour as well as the composition of the associated microbiome. Our results may improve patient stratification and pave the path for mechanistic studies on microbiota-tumour crosstalk.

En este trabajo se aborda la integralidad como idea–fuerza desde la cual se ha planificado la gestión del centro de extensión territorializado de la Facultad de Filosofía y Letras de la Universidad de Buenos Aires y la curricularización de la extensión mediante los Seminarios de Prácticas Socioeducativas Territorializadas. Las definiciones de política académica adoptadas implican un conjunto de mediaciones y dinámicas de gestión universitaria que se encuentran en el marco mayor de la educación superior como política pública, anclan en la experiencia histórica institucional y desafían las formas occidentales en que son pensadas y organizadas las unidades académicas. Así, la integralidad, en el caso de Facultad de Filosofía y Letras, configura un conjunto de múltiples movimientos interrelacionados y profundamente imbricados que superan con amplitud la integración de la extensión, docencia e investigación.

Carbonate-based electrolytes in Li-ion batteries exhibit long range order in a frozen state, which enables their non-destructive analysis by diffraction methods. In the current study the spatial distribution of lithium and electrolyte inside the graphite anode was determined in cycled Li-ion cells using monochromatic spatially-resolved neutron diffraction measurements at 150 K. The results indicate a loss of lithium and electrolyte and their non-uniform distribution in the graphite anode in aged Li-ion cells. The observed lithium and electrolyte losses are directly correlated with two electrochemical performance degradation mechanisms, which are responsible for the cell capacity fade.

The history of intellectuals consists of a long-spanning entangled web of influences, interdependencies, and inspirations. In this paper, we construe the history of intellectuals by means of a formalized network approach, and structurally analyze how communities form and develop based on their intellectual influences. We are working with a unique data set of Linked Open Data, which we critically reflect upon. In this paper we tackle the question of whether community detection can help us to identify schools of thought, as well as patterns in the influence relations of scholars. We provide a detailed description of the process of extracting Linked Open Data, the construction of longitudinal networks, and the methodology of identifying and evaluating intellectual communities in the dataset using a community detection algorithm. Finally, we track the dynamic evolution of these communities over time, and characterize the structural patterns of their evolution, and the mechanisms of their development. We contextualize the changes in selected network structures in order to establish the merit of this method for a new perspective on the history of intellectuals, their influences, and their ideas.