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2. Assessing the status of amphibian breeding sites in Italy: a national survey

Author

Andreone, Franco, Angelini, Jacopo, Associazione Natura Cascina Bellezza ONLUS, Associazione, Zirichiltaggi, Bassu, Lara, Bennati, R., Bernini, Franco, Bettiol, Katia, Biancardi, Biggi, Emanuele, Bionda, Radames, Bocca, Massimo, Bonato, Lucio, Buzzetti Filippo Maria, Carafa, Marco, Carletti, Silvia, Cassol, Michele, Casu, Viviana, Cavalieri, C., Cornetti, Luca, Corti, Claudia, Dal Lago Antonio, Damico, Maurizio, Delisio, Lorenzo, Delmastro Giovanni Battista, Di Cerbo Anna Rita, Di Francesco, N., Di Tizio Luciano, Donelli, O., Doria, Giuliano, Emiliani, Davide, Eusebio Bergò Paolo, Evangelista, Massimo, Faraone Francesco Paolo, Fattizzo, Tiziano, Ferri, Vincenzo, Ferro, M., Fiacchini, David, Ficetola, Francesco, Foglia, Gessica, Fulco, Egidio, Giacalone, G., Giberti, P., Gola, Grieco, Cristina, Guarino Fabio Maria, Iannelli, A., Iantorno, A., Incao, Jiménez Gonzales Pilar, Lamagni, Luca, Lillo, Francesco, Lo Valvo Mario, Manca, J., Marchesi, M., Mazzotti, Stefano, Menegon, Michele, Mezzasalma, Marcello, Miserocchi, Danio, Montioni, Francesca, Mosini, Andrea, Nistri Anna Maria, Nitti, Nicola, Novaga, R., Odierna, Gaetano, Oneto, Fabrizio, Ottonello, Dario, Parolin, Enrico, Pedrini, Paolo, Pellegrini, Mario, Pellitteri Rosa Daniele, Penazzi, Rocco, Petruzzi, F., Piazzini, Sandro, Picariello, Orfeo, Poggiani, Luciano, Pollo, R., Pupin, Fabio, Razzetti, Edoardo, Richard, Jacopo, Riservato, Elisa, Romanazzi, Enrico, Romano, Antonio, Romano Antonio Vito, Rossini, Marco, Sacchi, Roberto, Sala, Luigi, Salvidio, Sebastiano, Sassi, A., Scali, Stefano, Scirocco, T., Seglie, Daniele, Semenzato, Massimo, Silvano, Fabrizio, Sindaco, Roberto, Sommacal, Monica, Spada, Arianna, Sperone, Emilio, Spilinga, Cristiano, Svanella, Abbondio, Tormen, Fausto, Tormen, Giuseppe, Tripepi, Sandro, Vaccaro, Angelo, Vanni, Stefano, Ventrella, Pasquale, Nulchis, Valentina, Zampogno, Elena, Zuffi Marco, A. L., Salvidio, S., Andreone, F., Angelini, J. Bassu L., Bennati, R., Bernini, F., Bettiol, K., Biancardi, C, Biggi, E., Bionda, R., Bocca, M., Bonato, L., Buzzetti, Fm, Carafa, M., Carletti, S., Cassol, M., Casu, V., Cavalieri, C., Cornetti, L., Corti, C., Dal Lago, A., Damico, M., Delisio, L., Delmastro, Gb, Di Cerbo, A. R., Di Francesco, N., Di Tizio, L., Donelli, O., Doria, G., Emiliani, D., Eusebio, Bp, Eusebio Bergo, P., Evangelista, M., Faraone, F. P., Fattizzo, T., Ferri, V., Ferro, M., Fiacchini, D., Ficetola, F., Foglia, G., Fulco, E., Giacalone, G., Giberti, P., Gola, Grieco, C., Guarino, FABIO MARIA, Iannelli, A., Iantorno, A., Incao, Jiménez Gonzales, Pilar, Lamagni, L., Lillo, F., Lo Valvo, M., Manca, J., Marchesi, M., Mazzotti, S., Menegon, M., Mezzasalma, Marcello, Miserocchi, D., Montioni, F., Mosini, A., Nistri, A. M., Nitti, N., Novaga, R., Odierna, Gaetano, Oneto, F., Ottonello, D., Parolin, E., Pedrini, P., Pellegrini, M., Pellitteri Rosa, D., Penazzi, R., Petruzzi, F., Piazzini, S., Picariello, ORFEO LUCIO ANTONIO, Poggiani, L., Pollo, R., Pupin, F., Razzetti, E., Richard, J., Riservato, E., Romanazzi, E., Romano, A., Romano, A. V., Rossini, M., Sacchi, R., Sala, L., Sassi, A., Scali, S., Scirocco, T., Seglie, D., Semenzato, M., Silvano, F., Sindaco, R., Sommacal, M., Spada, A., Sperone, E., Spilinga, C., Svanella, A., Tormen, F., Tormen, G., Tripepi, S., Vaccaro, A., Vanni, S., Ventrella, P., Nulchis, V., Zampogno, E., and Zuffi, M. A. L. .

Published
2011

3. Assessing the status of amphibian breeding sites in Italy: a national survey

Author

Salvidio, Sebastian, Andreone, Franco, Angelini, Jacopo, Bassu, Lara, Bennati, R., Bernini, Franco, Bettiol, Katia, Biancardi, Biggi, Emanuele, Bionda, Radames, Bocca, Massimo, Bonato, Lucio, Buzzetti, Filippo Maria, Carafa, Marco, Carletti, Silvia, Cassol, Michele, Casu, Viviana, Cavalieri, C., Cornetti, Luca, Corti, Claudia, Dal Lago, Antonio, Damico, Maurizio, Delisio, Lorenzo, Delmastro, Giovanni Battista, Di Cerbo, Anna Rita, Di Francesco, N., Di Tizio, Luciano, Donelli, O., Doria, Giuliano, Emiliani, Davide, Eusebio, Bergo Paolo, Evangelista, Massimo, Faraone, Francesco Paolo, Fattizzo, Tiziano, Ferri, Vincenzo, Ferro, M., Fiacchini, David, Ficetola, Francesco, Foglia, Gessica, Fulco, Egidio, Giacalone, G., Giberti, P., Gola, Grieco, Cristina, Guarino, Fabio Maria, Iannelli, A., Iantorno, A., Incao, Jimenez, Gonzales Pilar, Lamagni, Luca, Lillo, Francesco, Lo Valvo, Mario, Manca, J., Marchesi, M., Mazzotti, Stefano, Menegon, Michele, Mezzasalma, Marcello, Miserocchi, Danio, Montioni, Francesca, Mosini, Andrea, Nistri, Anna Maria, Nitti, Nicola, Novaga, R., Odierna, Gaetano, Oneto, Fabrizio, Ottonello, Dario, Parolin, Enrico, Pedrini, Paolo, Pellegrini, Mario, Pellitteri-Rosa, Daniele, Penazzi, Rocco, Petruzzi, F., Piazzini, Sandro, Picariello, Orfeo, Poggiani, Luciano, Polio, R., Pupin, Fabio, Razzetti, Edoardo, Richard, Jacopo, Riservato, Elisa, Romanazzi, Enrico, Romano, Antonio, Romano, Antonio Vito, Rossini, Marco, Sacchi, Roberto, Sala, Luigi, Sassi, A., Scali, Stefano, Scirocco, T., Seglie, Daniele, Semenzato, Massimo, Silvano, Fabrizio, Sindaco, Roberto, Sommacal, Monica, Spada, Arianna, Emilio Sperone, Spilinga, Cristiano, Svanella, Abbondio, Tormen, Fausto, Tormen, Giuseppe, Tripepi, Sandro, Vaccaro, Angelo, Vanni, Stefano, Ventrella, Pasquale, Nulchis, Valentina, Zampogno, Elena, and Zuffi, Marco A. L.

Subjects
Amphibian breeding sites, Italian amphibian survey, conservation, freshwater habitat loss, scientific monitoring, volunteers
Published
2011

13. Assessing the status of amphibian breeding sites in Italy: a national survey

Author

Salvidio, S., Andreone, F., Angelini, J., Bassu, L., Bennati, R., Bernini, F., Bettiol, K., Biancardi, Biggi, E., Bionda, R., Bocca, M., Bonato, L., Buzzetti, F. M., Carafa, M., Carletti, S., Cassol, M., Casu, V., Cavalieri, C., Cornetti, L., Corti, C., Dal Lago, A., Damico, M., Delisio, L., Delmastro, G. B., Di Cerbo, A. R., Di Francesco, N., Di Tizio, L., Donelli, O., Doria, G., Emiliani, D., Eusebio, B. P., Evangelista, M., Faraone, F. P., Fattizzo, T., Ferri, V., Ferro, M., Fiacchini, D., Ficetola, F., Foglia, G., Fulco, E., Giacalone, G., Giberti, P., Gola, Grieco, C., Guarino, F. M., Iannelli, A., Iantorno, A., Incao, Jimenez, G. P., Lamagni, L., Lillo, F., Lo Valvo, M., Manca, J., Marchesi, M., Mazzotti, S., Menegon, M., Mezzasalma, M., Miserocchi, D., Montioni, F., Mosini, A., Nistri, A. M., Nitti, N., Novaga, R., Odierna, G., Oneto, F., Ottonello, D., Parolin, E., Pedrini, P., Pellegrini, M., Pellitteri-Rosa, D., Penazzi, R., Petruzzi, F., Piazzini, S., Picariello, O., Poggiani, L., Polio, R., Pupin, F., Razzetti, E., Richard, J., Riservato, E., Romanazzi, E., Romano, A., Romano, A. V., Rossini, M., Sacchi, R., Luigi Sala, Sassi, A., Scali, S., Scirocco, T., Seglie, D., Semenzato, M., Silvano, F., Sindaco, R., Sommacal, M., Spada, A., Sperone, E., Spilinga, C., Svanella, A., Tormen, F., Tormen, G., Tripepi, S., Vaccaro, A., Vanni, S., Ventrella, P., Nulchis, V., Zampogno, E., Zuffi, M. A. L., Soc Herpetologica, Italica, Assoc Nat Cascina Bellezza, Onlus, and Assoc, Zirichiltaggi

14. High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy.

Author

Bianco A, Normanno G, Capozzi L, Del Sambro L, Di Fato L, Miccolupo A, Di Taranto P, Caruso M, Petruzzi F, Ali A, and Parisi A

Abstract

The Bacillus cereus group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by B. cereus sensu lato ( s.l. ). One isolate per positive sample was characterized by Multilocus Sequence Typing (MLST) and for the presence of ten virulence genes. Based on MLST, all isolates were classified into 73 different sequence types (STs), of which 12 isolates were assigned to new STs. Virulence genes detection revealed that 90% and 61% of the isolates harboured the nheABC and the hblCDA gene cluster, respectively. Ninety-four percent of the isolates harboured the enterotoxin genes entS and entFM ; 8% of the isolates possessed the ces gene. Thirty-eight different genetic profiles were identified, suggesting a high genetic diversity. Our study clearly shows the widespread diffusion of potentially toxigenic isolates of B. cereus s.l. in milk and cheeses in the Apulia region highlighting the need to adopt GMP and HACCP procedures along every step of the milk and cheese production chain in order to reduce the public health risk linked to the consumption of foods contaminated by B. cereus s.l.

Published
2023
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15. Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry.

Author

Manzulli V, Rondinone V, Buchicchio A, Serrecchia L, Cipolletta D, Fasanella A, Parisi A, Difato L, Iatarola M, Aceti A, Poppa E, Tolve F, Pace L, Petruzzi F, Rovere ID, Raele DA, Del Sambro L, Giangrossi L, and Galante D

Abstract

Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry (MALDI-TOF MS) technology is currently increasingly used in diagnostic laboratories as a cost effective, rapid and reliable routine technique for the identification and typing of microorganisms. In this study, we used MALDI-TOF MS to analyze a collection of 160 strains belonging to the Bacillus cereus group (57 B. anthracis , 49 B. cereus , 1 B. mycoides , 18 B. wiedmannii , 27 B. thuringiensis , 7 B. toyonensis and 1 B. weihenstephanensis ) and to detect specific biomarkers which would allow an unequivocal identification. The Main Spectra Profiles (MSPs) were added to an in-house reference library, expanding the current commercial library which does not include B. toyonensis and B. wiedmannii mass spectra. The obtained mass spectra were statistically compared by Principal Component Analysis (PCA) that revealed seven different clusters. Moreover, for the identification purpose, were generated dedicate algorithms for a rapid and automatic detection of characteristic ion peaks after the mass spectra acquisition. The presence of specific biomarkers can be used to differentiate strains within the B. cereus group and to make a reliable identification of Bacillus anthracis , etiologic agent of anthrax, which is the most pathogenic and feared bacterium of the group. This could offer a critical time advantage for the diagnosis and for the clinical management of human anthrax even in case of bioterror attacks.

Published
2021
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16. VOC 202012/01 Variant Is Effectively Neutralized by Antibodies Produced by Patients Infected before Its Diffusion in Italy.

Author

Rondinone V, Pace L, Fasanella A, Manzulli V, Parisi A, Capobianchi MR, Ostuni A, Chironna M, Caprioli E, Labonia M, Cipolletta D, Della Rovere I, Serrecchia L, Petruzzi F, Pennuzzi G, and Galante D

Subjects
Animals, COVID-19 epidemiology, Chlorocebus aethiops, Humans, Italy, Neutralization Tests, Pandemics, United Kingdom, Vero Cells, Antibodies, Neutralizing blood, Antibodies, Viral blood, COVID-19 immunology, SARS-CoV-2 immunology
Abstract

The coronavirus disease 2019 (Covid-19) pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and presents a global health emergency that needs urgent intervention. Viruses constantly change through mutation, and new variants of a virus are expected to occur over time. In the United Kingdom (UK), a new variant called B.1.1.7 has emerged with an unusually large number of mutations. The aim of this study is to evaluate the level of protection of sera from 12 patients infected and later healed in Apulia Region (Italy) with Covid-19 between March and November 2020, when the English variant was not circulating in this territory yet, against the new VOC 202012/01 variant by seroneutralization assay. The sera of patients had already been tested before, using a virus belonging to the lineage B.1 and showed an antibody neutralizing titer ranging between 1:160 and 1:320. All the 12 sera donors confirmed the same titers of neutralizing antibodies obtained with a strain belonging to the lineage B.1.1.7 (VOC 202012/01). These data indicate that antibodies produced in subjects infected with variants of Sars-CoV-2 strain before the appearance of the English one, seem to have a neutralizing power also against this variant.

Published
2021
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17. [Use of flunitrazepam during epidural anesthesia].

Author

Fierro G, Valente G, Spadaro G, Petruzzi F, and De Pinto N

Subjects
Adult, Aged, Blood Pressure drug effects, Drug Evaluation, Female, Heart Rate drug effects, Humans, Male, Middle Aged, Respiration drug effects, Time Factors, Anesthesia, Epidural methods, Anti-Anxiety Agents administration & dosage, Flunitrazepam administration & dosage
Published
1982

18. [Use of hyperbaric oxygen therapy in surgery].

Author

Valente G, Fierro G, Costanzo C, Pelaia P, Boccianti R, Cottini F, and Petruzzi F

Subjects
Arterial Occlusive Diseases therapy, Crush Syndrome therapy, Embolism, Air therapy, Free Radicals, Gangrene therapy, Gas Gangrene therapy, Humans, Ischemia therapy, Oxygen blood, Hyperbaric Oxygenation
Published
1987

19. [Retrocaval ureter].

Author

Stella S, Redler A, Nemec S, and Petruzzi F

Subjects
Adult, Female, Humans, Hydronephrosis etiology, Radiography, Ureter diagnostic imaging, Ureter surgery, Ureter abnormalities
Published
1979

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