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24 results on '"Petrovic, Isidora"'

5. Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells

Author

Lazic, Stefan, primary, Duzanic, Filip, additional, Stanisavljevic Ninkovic, Danijela, additional, Drakulic, Danijela, additional, Mojsin, Marija, additional, Milivojevic, Milena, additional, Balint, Vanda, additional, Petrovic, Isidora, additional, Kovacevic Grujicic, Natasa, additional, Schwirtlich, Marija, additional, and Stevanovic, Milena, additional

Published
2022
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10. Apigenin-7-O-glucoside versus apigenin

Author

Smiljkovic, Marija, Stanisavljevic, Danijela, Stojkovic, Dejan, Petrovic, Isidora, Marjanovic Vicentic, Jelena, Popovic, Jelena, Golic Grdadolnik, Simona, Markovic, Dejan, Sanković-Babić, Snežana, Glamoclija, Jasmina, Stevanovic, Milena, and Sokovic, Marina

Subjects
apigenin, cytotoxic, apigenin-7-O-glucoside, HCT116, antifungal, Candida spp
Abstract

Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin-7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell’s viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used., EXCLI Journal;Vol. 16 2017

Published
2017

23. Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals.

Author

Kovacevic-Grujicic, Natasa, Mojsin, Marija, Djurovic, Jelena, Petrovic, Isidora, and Stevanovic, Milena

Subjects
PROTEINS, TRANSCRIPTION factors, GENOMICS, PROMOTERS (Genetics), HUMAN genetics
Abstract

SOX proteins constitute a large family of diverse and well conserved transcription factors implicated in the control of various developmental processes. Previously we have cloned and characterized human SOX3, SOX14 and SOX18 genes and performed functional characterization of their promoter regions. To better understand organization and function of SOX3, SOX14 and SOX18 promoters and to determine evolutionary conserved regulatory regions, we performed comparative genomic analyses of orthologous genes promoters. Mammalian orthologs of the human SOX3, SOX14 and SOX18 genes show high sequence identity in their promoter regions, particularly within basal promoters of the respective human genes. Binding sites for transcription factors NF-Y, Sp1 and USF1, previously shown to play critical roles in transcriptional regulation of these human genes, are highly conserved in sequence and position among diverse mammalian species. Conservation of binding sites might indicate their highly significant roles in maintaining the transcriptional regulation of these genes among different species. [ABSTRACT FROM AUTHOR]

Published
2008
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24. Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions.

Author

Smiljkovic M, Stanisavljevic D, Stojkovic D, Petrovic I, Marjanovic Vicentic J, Popovic J, Golic Grdadolnik S, Markovic D, Sankovic-Babice S, Glamoclija J, Stevanovic M, and Sokovic M

Abstract

Bioactive potential of apigenin derivative apigenin-7- O -glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin-7- O -glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7- O -glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7- O -glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7- O -glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7- O -glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7- O -glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7- O -glucoside has more potent activity compared to apigenin in all assays that we used.

Published
2017
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