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7. Persistence of Chronic Lymphocytic Leukemia Stem-like Populations under Simultaneous In Vitro Treatment with Curcumin, Fludarabine, and Ibrutinib: Implications for Therapy Resistance

Author

Bistué-Rovira, Àngel, primary, Rico, Laura G., additional, Bardina, Jorge, additional, Juncà, Jordi, additional, Granada, Isabel, additional, Bradford, Jolene A., additional, Ward, Michael D., additional, Salvia, Roser, additional, Solé, Francesc, additional, and Petriz, Jordi, additional

Published
2024
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11. Fast-screening flow cytometry method for detecting nanoplastics in human peripheral blood

Author

Salvia, Roser, primary, Rico, Laura G., additional, Bradford, Jolene A., additional, Ward, Michael D., additional, Olszowy, Michael W., additional, Martínez, Cristina, additional, Madrid-Aris, Álvaro Domingo, additional, Grífols, Joan R., additional, Ancochea, Águeda, additional, Gomez-Muñoz, Laia, additional, Vives-Pi, Marta, additional, Martínez-Cáceres, Eva, additional, Fernández, Marco A., additional, Sorigue, Marc, additional, and Petriz, Jordi, additional

Published
2023
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18. Accurate identification of cell doublet profiles: Comparison of light scattering with fluorescence measurement techniques.

Author

Rico, Laura G., Bardina, Jorge, Bistué‐Rovira, Àngel, Salvia, Roser, Ward, Mike D., Bradford, Jolene A., and Petriz, Jordi

Abstract

Doublet discrimination is usually based on pulse analysis of light scatter parameters. A combination of two pulse parameters (Area, A; Height, H; or Width, W) can be used to discriminate a pulse originated in a single cell from a pulse originated from cells stuck together. Fluorescence signals can be also used to discriminate aggregates, being essential to identify cells in the G2/M phase from doublets in the G0/G1 phase in cell cycle/DNA applications. The most used method combines FSC‐A versus FSC‐H, whereas other strategies combine FSC‐H versus FSC‐W, SSC‐H versus SSC‐A and SSC‐H versus SSC‐W. However, when studying activated or proliferating cells, scatter discrimination can be difficult. In this study, we have compared the use of light scattering with fluorescence measurement techniques for successful doublet discrimination for single cells. Effective use of FSC and SSC height, area and width are commonly used to eliminate aggregates. However, fluorescence‐based methods using viable DNA stains provide a good compromise between performance and accurate manual gating methods, especially for highly concentrated cell products and pathological specimens. Viable DNA dyes, such as Vybrant™ DyeCycle™ Violet stain or Hoechst 33342, can be used to detect nucleated cells in blood and in bone marrow, or to discriminate cell aggregates and debris based on no‐lyse no‐wash assays, where scatter degradation is a dominant component of the measured data, which increases with event rate. [ABSTRACT FROM AUTHOR]

Published
2023
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19. Erythrocyte lysing solutions have a detrimental effect in flow cytometric dendritic cell detection.

Author

Rico, Laura G., Salvia, Roser, Juncà, Jordi, Ward, Mike D., Bradford, Jolene A., Sorigue, Marc, and Petriz, Jordi

Abstract

Flow cytometry (FCM) enumeration of peripheral blood dendritic cells (PBDCs) is a minimally invasive procedure extremely useful for immunological studies. Numbers of PBDCs vary depending on age, lifestyle, or in pathologies like cancer, leukemia or immunodeficiencies. Conventional methods for PBDC identification by FCM involve red blood cell lysis using either formaldehyde or ammonium chloride‐based solutions. This specific procedure has been widely reported to cause a detrimental effect as well as an artifactual detection of target populations. Alternatively, minimal sample perturbation assays that avoid the use of erythrolytic solutions with centrifugation steps and preserve the native cellular state are simpler and more robust than conventional methods. In this study, we aimed to evaluate how conventional FCM assays can alter dendritic cell (DC) counting when compared with minimal sample perturbation protocols, in terms of absolute cell counting, percentage and stain index (SI) of PBDC subsets. We evaluated the use of three different erythrolytic solutions (CyLyse, OptiLyse C, and Pharm Lyse) on a series of n = 20 peripheral blood specimens for conventional and plasmacytoid DCs detection as well as for leukocyte and basophil detection. Our results showed a significant reduction of leukocytes and specifically, of DCs and basophils in terms of absolute number when using erythrolytic solutions. In conclusion, our study shows that PBDC counting is heavily affected when lysing solutions are used, indicating that these stellate‐shaped populations appear to be more labile. [ABSTRACT FROM AUTHOR]

Published
2023
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20. Impact of red blood cell lysing on rare event analysis.

Author

Rico, Laura G., de la Calle, Fernando Raimúndez, Salvia, Roser, Ward, Mike D., Bradford, Jolene A., Juncà, Jordi, Sorigue, Marc, and Petriz, Jordi

Abstract

The challenges associated with analyzing rare cells are dependent on a series of factors, which usually require large numbers of cells per sample for successful resolution. Among these is determining the minimum number of total events needed to be acquired as defined by the expected frequency of the target cell population. The choice of markers that identify the target population, as well as the event rate and the number of aborted events/second, will also determine the statistically significant detection of rare cell events. Sample preparation is another important but often overlooked factor in rare cell analysis, and in this study we examine Poisson theory and methods to determine the effect of sample manipulation on rare cell detection. After verifying the applicability of this theory, we have evaluated the potential impact of red cell lysis on rare cell analysis, and how cell rarity can be underestimated or overestimated based on erythrolytic sensitivity or resistance of healthy leukocytes and pathological rare cells. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Individual Quality Assessment of Autografting by Probability Estimation for Clinical Endpoints: A Prospective Validation Study from the European Group for Blood and Marrow Transplantation

Author

Lanza, Francesco, Campioni, Diana C., Hellmann, Andrzej, Milone, Giuseppe, Wahlin, Anders, Walewski, Jan, Spedini, Pierangelo, Fiamenghi, Cristina, Cuneo, Antonio, Knopińska, Wanda, Swierkowska-Czeneszew, Monica, Petriz, Jordi, Fruehauf, Stefan, Farge, Dominique, Mohty, Mohamad, Passweg, Jacob, Ruuto, Tapani, Madrigal, Alejandro, and Johnsen, Hans E.

Published
2013
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25. Més a prop de la quantificació de la resposta immunitària específica front al càncer i les malalties infeccioses

Author

Petriz, Jordi

Abstract

En els tractaments contra el càncer o de les malalties infeccioses és molt important poder determinar l'activitat citotòxica cel·lular, és a dir, la capacitat defensiva que té el sistema immunitari enfront d'una cèl·lula alterada. No obstant això, la manipulació de mostres biològiques, a més de tedioses, comprometen el correcte funcionament de les cèl·lules en el moment de ser estudiades. En aquest article Jordi Petriz presenta un nou mètode que permet estudiar mostres recentment extretes minimitzant l'impacte del seu maneig, a més de reduir el temps a només dues hores. En los tratamientos contra el cáncer o de las enfermedades infecciosas es muy importante poder determinar la actividad citotóxica celular, es decir, la capacidad defensiva que tiene el sistema inmunitario frente a una célula alterada. Sin embargo, la manipulación de muestras biológicas, además de tediosas, comprometen el correcto funcionamiento de las células en el momento de ser estudiadas. En este artículo Jordi Petriz presenta un nuevo método que permite estudiar muestras recién extraídas minimizando el impacto de su manejo, además de reducir el tiempo a sólo dos horas.

Published
2021

26. Cap a l'automatització de l'anàlisi d'un enzim clau en medicina : la fosfatasa alcalina granulocítica

Author

Petriz, Jordi

Abstract

Alguns mètodes de diagnòstic emprats en recerca i en la pràctica clínica habitual s'han mantingut pràcticament invariables durant dècades. Un grup d'investigació de l'Institut de Recerca Contra la Leucèmia Josep Carreras, ha desenvolupat un nou mètode per a la determinació de l'activitat fosfatasa alcalina granulocítica. Es tracta d'un avenç que permet la quantificació d'aquesta activitat enzimàtica d'una manera molt ràpida i reproduïble, com un pas molt important cap a la seva automatització. Algunos métodos de diagnóstico empleados en investigación y en la práctica clínica habitual se han mantenido prácticamente invariables durante décadas. Un grupo de investigación del Instituto de Investigación Contra la Leucemia Josep Carreras, ha desarrollado un nuevo método para la determinación de la actividad fosfatasa alcalina granulocítica. Se trata de un adelanto que permite la cuantificación de esta actividad enzimática de una manera muy rápida y reproducible, como un paso muy importante hacia su automatización.

Published
2021

27. Unmasking the expression of PD-L1 in Myeloid Derived Suppressor Cells: A case study in lung cancer to discover new drugs with specific on-target efficacy

Author

G. Rico, Laura, Aguilar-Hernández, Andrés, Ward, M. D., Bradford, Jolene A, Junca, Jordi, Rosell, Rafael, Petriz, Jordi, and Universitat Autònoma de Barcelona

Subjects
0301 basic medicine, PD-L1, Cancer Research, Conformational change, medicine.medical_treatment, MDSCs, Stimulation, lcsh:RC254-282, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, medicine, Lung cancer, Letters to the Editor, medicine.diagnostic_test, biology, Mechanism (biology), Chemistry, Immunotherapy, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Cancer research, biology.protein, Myeloid-derived Suppressor Cell
Abstract

• PD-L1 displays variation of spatial conformation in response to phorbol ester stimulation, which may confer a critical enhancement in binding affinity. • PD-L1 conformational change may be associated with an immunoregulatory mechanism that affects therapies targeting the PD-1/PD-L1 checkpoint. • Eliminating MDSCs by promoting PD-L1 stabilized unfolded states on both PMN- and M-MDSCs could improve immunotherapy efficacy. • In view of these conformational properties, analysis of accumulation, expansion and survival of pathological immunosuppressive MDSCs could help to better understand and overcome the mechanisms of immunotherapy resistance, by developing new treatment strategies aimed at promoting PD-L1 stabilized unfolded states.

Published
2020

29. Més a prop d'identificar les cèl·lules mare leucèmiques

Author

Petriz, Jordi

Abstract

La citometria de flux és una tecnologia que permetria identificar i aïllar cèl·lules mare malignes molt minoritàries com les leucèmiques, responsables de la recaiguda i la resistència als tractaments quimioterapèutics, enfront de la manca de marcadors genètics i fenotípics universals que ho posin en relació. En aquest sentit, un grup investigador de l'Institut de recerca contra la Leucèmia Josep Carreras ha desenvolupat un assaig diagnòstic innovador tenint en compte la funcionalitat enzimàtica de les cèl·lules, concretament de l'activitat fosfatasa alcalina. Reconèixer-les en diagnosticar la leucèmia milloraria el pronòstic de pacients d'alt risc i l'aplicació i desenvolupament de teràpies més efectives. La citometría de flujo es una tecnología que permitiría identificar y aislar células madre malignas muy minoritarias como las leucémicas, responsables de la recaída y la resistencia a los tratamientos quimioterapéuticos, frente a la falta de marcadores genéticos y fenotípicos universales que lo pongan en relación. En este sentido, un grupo investigador del Instituto de investigación contra la Leucemia Josep Carreras ha desarrollado un ensayo diagnóstico innovador teniendo en cuenta la funcionalidad enzimática de las células, concretamente de la actividad fosfatasa alcalina. Reconocerlas en diagnosticar la leucemia mejoraría el pronóstico de pacientes de alto riesgo y la aplicación y desarrollo de terapias más efectivas. Flow cytometry is a technology that would allow the identification and isolation of very minority malignant stem cells, such as leukemias, responsible for relapse and resistance to chemotherapeutic treatments, due to the lack of universal genetic and phenotypic markers that relate them. In this sense, a research group at the Josep Carreras Leukemia Research Institute has developed an innovative diagnostic test, taking into account the enzymatic functionality of the cells, in particular the alkaline phosphatase activity. Recognizing them in diagnosing leukemia would improve the prognosis for high-risk patients and the application and development of more effective therapies.

Published
2020

32. Diagnostic performance of the ClearLLab 10C B cell tube

Author

Espasa, Andrea, primary, Torrents, Silvia, additional, Morales‐Indiano, Cristian, additional, Rico, Laura G., additional, Bardina, Jorge, additional, Ancochea, Agueda, additional, Bistué‐Rovira, Àngel, additional, Linio, Rosa, additional, Raya, Minerva, additional, Vergara, Sara, additional, Juncà, Jordi, additional, Grifols, Joan‐Ramon, additional, Petriz, Jordi, additional, Soria, Maria‐Gloria, additional, and Sorigue, Marc, additional

Published
2020
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36. Flow cytometry counting of CD34+ cells in whole blood

Author

Fornas, Oscar, Garcia, Joan, and Petriz, Jordi

Subjects
Cell surface antigens -- Physiological aspects, Cell surface antigens -- Research, Flow cytometry -- Methods, Flow cytometry -- Usage, Hematopoietic stem cells -- Measurement, Hematopoietic stem cells -- Research
Abstract

Author(s): Oscar Fornas [1]; Joan Garcia [1]; Jordi Petriz (corresponding author) [1, 2] Hematopoietic stem cells are able to self-renew and to differentiate into progenitors of all hematopoietic lineages [1, [...]

Published
2000
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38. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author

Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Acs, Andreas, additional, Adam, Dieter, additional, Adam‐Klages, Sabine, additional, Agace, William W., additional, Aghaeepour, Nima, additional, Akdis, Mübeccel, additional, Allez, Matthieu, additional, Almeida, Larissa Nogueira, additional, Alvisi, Giorgia, additional, Anderson, Graham, additional, Andrä, Immanuel, additional, Annunziato, Francesco, additional, Anselmo, Achille, additional, Bacher, Petra, additional, Baldari, Cosima T., additional, Bari, Sudipto, additional, Barnaba, Vincenzo, additional, Barros‐Martins, Joana, additional, Battistini, Luca, additional, Bauer, Wolfgang, additional, Baumgart, Sabine, additional, Baumgarth, Nicole, additional, Baumjohann, Dirk, additional, Baying, Bianka, additional, Bebawy, Mary, additional, Becher, Burkhard, additional, Beisker, Wolfgang, additional, Benes, Vladimir, additional, Beyaert, Rudi, additional, Blanco, Alfonso, additional, Boardman, Dominic A., additional, Bogdan, Christian, additional, Borger, Jessica G., additional, Borsellino, Giovanna, additional, Boulais, Philip E., additional, Bradford, Jolene A., additional, Brenner, Dirk, additional, Brinkman, Ryan R., additional, Brooks, Anna E. S., additional, Busch, Dirk H., additional, Büscher, Martin, additional, Bushnell, Timothy P., additional, Calzetti, Federica, additional, Cameron, Garth, additional, Cammarata, Ilenia, additional, Cao, Xuetao, additional, Cardell, Susanna L., additional, Casola, Stefano, additional, Cassatella, Marco A., additional, Cavani, Andrea, additional, Celada, Antonio, additional, Chatenoud, Lucienne, additional, Chattopadhyay, Pratip K., additional, Chow, Sue, additional, Christakou, Eleni, additional, Čičin‐Šain, Luka, additional, Clerici, Mario, additional, Colombo, Federico S., additional, Cook, Laura, additional, Cooke, Anne, additional, Cooper, Andrea M., additional, Corbett, Alexandra J., additional, Cosma, Antonio, additional, Cosmi, Lorenzo, additional, Coulie, Pierre G., additional, Cumano, Ana, additional, Cvetkovic, Ljiljana, additional, Dang, Van Duc, additional, Dang‐Heine, Chantip, additional, Davey, Martin S., additional, Davies, Derek, additional, De Biasi, Sara, additional, Del Zotto, Genny, additional, Dela Cruz, Gelo Victoriano, additional, Delacher, Michael, additional, Della Bella, Silvia, additional, Dellabona, Paolo, additional, Deniz, Günnur, additional, Dessing, Mark, additional, Di Santo, James P., additional, Diefenbach, Andreas, additional, Dieli, Francesco, additional, Dolf, Andreas, additional, Dörner, Thomas, additional, Dress, Regine J., additional, Dudziak, Diana, additional, Dustin, Michael, additional, Dutertre, Charles‐Antoine, additional, Ebner, Friederike, additional, Eckle, Sidonia B. G., additional, Edinger, Matthias, additional, Eede, Pascale, additional, Ehrhardt, Götz R.A., additional, Eich, Marcus, additional, Engel, Pablo, additional, Engelhardt, Britta, additional, Erdei, Anna, additional, Esser, Charlotte, additional, Everts, Bart, additional, Evrard, Maximilien, additional, Falk, Christine S., additional, Fehniger, Todd A., additional, Felipo‐Benavent, Mar, additional, Ferry, Helen, additional, Feuerer, Markus, additional, Filby, Andrew, additional, Filkor, Kata, additional, Fillatreau, Simon, additional, Follo, Marie, additional, Förster, Irmgard, additional, Foster, John, additional, Foulds, Gemma A., additional, Frehse, Britta, additional, Frenette, Paul S., additional, Frischbutter, Stefan, additional, Fritzsche, Wolfgang, additional, Galbraith, David W., additional, Gangaev, Anastasia, additional, Garbi, Natalio, additional, Gaudilliere, Brice, additional, Gazzinelli, Ricardo T., additional, Geginat, Jens, additional, Gerner, Wilhelm, additional, Gherardin, Nicholas A., additional, Ghoreschi, Kamran, additional, Gibellini, Lara, additional, Ginhoux, Florent, additional, Goda, Keisuke, additional, Godfrey, Dale I., additional, Goettlinger, Christoph, additional, González‐Navajas, Jose M., additional, Goodyear, Carl S., additional, Gori, Andrea, additional, Grogan, Jane L., additional, Grummitt, Daryl, additional, Grützkau, Andreas, additional, Haftmann, Claudia, additional, Hahn, Jonas, additional, Hammad, Hamida, additional, Hämmerling, Günter, additional, Hansmann, Leo, additional, Hansson, Goran, additional, Harpur, Christopher M., additional, Hartmann, Susanne, additional, Hauser, Andrea, additional, Hauser, Anja E., additional, Haviland, David L., additional, Hedley, David, additional, Hernández, Daniela C., additional, Herrera, Guadalupe, additional, Herrmann, Martin, additional, Hess, Christoph, additional, Höfer, Thomas, additional, Hoffmann, Petra, additional, Hogquist, Kristin, additional, Holland, Tristan, additional, Höllt, Thomas, additional, Holmdahl, Rikard, additional, Hombrink, Pleun, additional, Houston, Jessica P., additional, Hoyer, Bimba F., additional, Huang, Bo, additional, Huang, Fang‐Ping, additional, Huber, Johanna E., additional, Huehn, Jochen, additional, Hundemer, Michael, additional, Hunter, Christopher A., additional, Hwang, William Y. K., additional, Iannone, Anna, additional, Ingelfinger, Florian, additional, Ivison, Sabine M, additional, Jäck, Hans‐Martin, additional, Jani, Peter K., additional, Jávega, Beatriz, additional, Jonjic, Stipan, additional, Kaiser, Toralf, additional, Kalina, Tomas, additional, Kamradt, Thomas, additional, Kaufmann, Stefan H. E., additional, Keller, Baerbel, additional, Ketelaars, Steven L. C., additional, Khalilnezhad, Ahad, additional, Khan, Srijit, additional, Kisielow, Jan, additional, Klenerman, Paul, additional, Knopf, Jasmin, additional, Koay, Hui‐Fern, additional, Kobow, Katja, additional, Kolls, Jay K., additional, Kong, Wan Ting, additional, Kopf, Manfred, additional, Korn, Thomas, additional, Kriegsmann, Katharina, additional, Kristyanto, Hendy, additional, Kroneis, Thomas, additional, Krueger, Andreas, additional, Kühne, Jenny, additional, Kukat, Christian, additional, Kunkel, Désirée, additional, Kunze‐Schumacher, Heike, additional, Kurosaki, Tomohiro, additional, Kurts, Christian, additional, Kvistborg, Pia, additional, Kwok, Immanuel, additional, Landry, Jonathan, additional, Lantz, Olivier, additional, Lanuti, Paola, additional, LaRosa, Francesca, additional, Lehuen, Agnès, additional, LeibundGut‐Landmann, Salomé, additional, Leipold, Michael D., additional, Leung, Leslie Y.T., additional, Levings, Megan K., additional, Lino, Andreia C., additional, Liotta, Francesco, additional, Litwin, Virginia, additional, Liu, Yanling, additional, Ljunggren, Hans‐Gustaf, additional, Lohoff, Michael, additional, Lombardi, Giovanna, additional, Lopez, Lilly, additional, López‐Botet, Miguel, additional, Lovett‐Racke, Amy E., additional, Lubberts, Erik, additional, Luche, Herve, additional, Ludewig, Burkhard, additional, Lugli, Enrico, additional, Lunemann, Sebastian, additional, Maecker, Holden T., additional, Maggi, Laura, additional, Maguire, Orla, additional, Mair, Florian, additional, Mair, Kerstin H., additional, Mantovani, Alberto, additional, Manz, Rudolf A., additional, Marshall, Aaron J., additional, Martínez‐Romero, Alicia, additional, Martrus, Glòria, additional, Marventano, Ivana, additional, Maslinski, Wlodzimierz, additional, Matarese, Giuseppe, additional, Mattioli, Anna Vittoria, additional, Maueröder, Christian, additional, Mazzoni, Alessio, additional, McCluskey, James, additional, McGrath, Mairi, additional, McGuire, Helen M., additional, McInnes, Iain B., additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Miller, Stephen D., additional, Mills, Kingston H.G., additional, Minderman, Hans, additional, Mjösberg, Jenny, additional, Moore, Jonni, additional, Moran, Barry, additional, Moretta, Lorenzo, additional, Mosmann, Tim R., additional, Müller, Susann, additional, Multhoff, Gabriele, additional, Muñoz, Luis Enrique, additional, Münz, Christian, additional, Nakayama, Toshinori, additional, Nasi, Milena, additional, Neumann, Katrin, additional, Ng, Lai Guan, additional, Niedobitek, Antonia, additional, Nourshargh, Sussan, additional, Núñez, Gabriel, additional, O'Connor, José‐Enrique, additional, Ochel, Aaron, additional, Oja, Anna, additional, Ordonez, Diana, additional, Orfao, Alberto, additional, Orlowski‐Oliver, Eva, additional, Ouyang, Wenjun, additional, Oxenius, Annette, additional, Palankar, Raghavendra, additional, Panse, Isabel, additional, Pattanapanyasat, Kovit, additional, Paulsen, Malte, additional, Pavlinic, Dinko, additional, Penter, Livius, additional, Peterson, Pärt, additional, Peth, Christian, additional, Petriz, Jordi, additional, Piancone, Federica, additional, Pickl, Winfried F., additional, Piconese, Silvia, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Podolska, Malgorzata Justyna, additional, Poon, Zhiyong, additional, Pracht, Katharina, additional, Prinz, Immo, additional, Pucillo, Carlo E. M., additional, Quataert, Sally A., additional, Quatrini, Linda, additional, Quinn, Kylie M., additional, Radbruch, Helena, additional, Radstake, Tim R. D. J., additional, Rahmig, Susann, additional, Rahn, Hans‐Peter, additional, Rajwa, Bartek, additional, Ravichandran, Gevitha, additional, Raz, Yotam, additional, Rebhahn, Jonathan A., additional, Recktenwald, Diether, additional, Reimer, Dorothea, additional, Reis e Sousa, Caetano, additional, Remmerswaal, Ester B.M., additional, Richter, Lisa, additional, Rico, Laura G., additional, Riddell, Andy, additional, Rieger, Aja M., additional, Robinson, J. Paul, additional, Romagnani, Chiara, additional, Rubartelli, Anna, additional, Ruland, Jürgen, additional, Saalmüller, Armin, additional, Saeys, Yvan, additional, Saito, Takashi, additional, Sakaguchi, Shimon, additional, Sala‐de‐Oyanguren, Francisco, additional, Samstag, Yvonne, additional, Sanderson, Sharon, additional, Sandrock, Inga, additional, Santoni, Angela, additional, Sanz, Ramon Bellmàs, additional, Saresella, Marina, additional, Sautes‐Fridman, Catherine, additional, Sawitzki, Birgit, additional, Schadt, Linda, additional, Scheffold, Alexander, additional, Scherer, Hans U., additional, Schiemann, Matthias, additional, Schildberg, Frank A., additional, Schimisky, Esther, additional, Schlitzer, Andreas, additional, Schlosser, Josephine, additional, Schmid, Stephan, additional, Schmitt, Steffen, additional, Schober, Kilian, additional, Schraivogel, Daniel, additional, Schuh, Wolfgang, additional, Schüler, Thomas, additional, Schulte, Reiner, additional, Schulz, Axel Ronald, additional, Schulz, Sebastian R., additional, Scottá, Cristiano, additional, Scott‐Algara, Daniel, additional, Sester, David P., additional, Shankey, T. Vincent, additional, Silva‐Santos, Bruno, additional, Simon, Anna Katharina, additional, Sitnik, Katarzyna M., additional, Sozzani, Silvano, additional, Speiser, Daniel E., additional, Spidlen, Josef, additional, Stahlberg, Anders, additional, Stall, Alan M., additional, Stanley, Natalie, additional, Stark, Regina, additional, Stehle, Christina, additional, Steinmetz, Tobit, additional, Stockinger, Hannes, additional, Takahama, Yousuke, additional, Takeda, Kiyoshi, additional, Tan, Leonard, additional, Tárnok, Attila, additional, Tiegs, Gisa, additional, Toldi, Gergely, additional, Tornack, Julia, additional, Traggiai, Elisabetta, additional, Trebak, Mohamed, additional, Tree, Timothy I.M., additional, Trotter, Joe, additional, Trowsdale, John, additional, Tsoumakidou, Maria, additional, Ulrich, Henning, additional, Urbanczyk, Sophia, additional, van de Veen, Willem, additional, van den Broek, Maries, additional, van der Pol, Edwin, additional, Van Gassen, Sofie, additional, Van Isterdael, Gert, additional, van Lier, René A.W., additional, Veldhoen, Marc, additional, Vento‐Asturias, Salvador, additional, Vieira, Paulo, additional, Voehringer, David, additional, Volk, Hans‐Dieter, additional, von Borstel, Anouk, additional, von Volkmann, Konrad, additional, Waisman, Ari, additional, Walker, Rachael V., additional, Wallace, Paul K., additional, Wang, Sa A., additional, Wang, Xin M., additional, Ward, Michael D., additional, Ward‐Hartstonge, Kirsten A, additional, Warnatz, Klaus, additional, Warnes, Gary, additional, Warth, Sarah, additional, Waskow, Claudia, additional, Watson, James V., additional, Watzl, Carsten, additional, Wegener, Leonie, additional, Weisenburger, Thomas, additional, Wiedemann, Annika, additional, Wienands, Jürgen, additional, Wilharm, Anneke, additional, Wilkinson, Robert John, additional, Willimsky, Gerald, additional, Wing, James B., additional, Winkelmann, Rieke, additional, Winkler, Thomas H., additional, Wirz, Oliver F., additional, Wong, Alicia, additional, Wurst, Peter, additional, Yang, Jennie H. M., additional, Yang, Juhao, additional, Yazdanbakhsh, Maria, additional, Yu, Liping, additional, Yue, Alice, additional, Zhang, Hanlin, additional, Zhao, Yi, additional, Ziegler, Susanne Maria, additional, Zielinski, Christina, additional, Zimmermann, Jakob, additional, and Zychlinsky, Arturo, additional

Published
2019
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41. Flow cytometric significance of cellular alkaline phosphatase activity in acute myeloid leukemia

Author

G. Rico, Laura, Junca, Jordi, Ward, Michael D., Bradford, Jolene A., Petriz, Jordi, and Universitat Autònoma de Barcelona

Subjects
0301 basic medicine, medicine.medical_specialty, CD34, Stem cells, acute myeloid leukemia, leukemic stem cells, Group A, Gastroenterology, Group B, 03 medical and health sciences, 0302 clinical medicine, stem cells, Internal medicine, Alkaline phosphatase, medicine, Leukemic stem cells, Acute myeloid leukemia, business.industry, Myeloid leukemia, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Molecular Profile, Stem cell, business, alkaline phosphatase, Research Paper, Cohort study
Abstract

// Laura G. Rico 1 , Jordi Junca 1 , 2 , Michael D. Ward 3 , Jolene A. Bradford 3 and Jordi Petriz 1 1 Functional Cytomics Group, Institut de Recerca contra la Leucemia Josep Carreras, IJC Campus ICO-Germans Trias i Pujol, Institut Germans Trias i Pujol (IGTP), Universitat Autonoma de Barcelona, UAB, Badalona, Spain 2 Institut Catala d’Oncologia, Hospital Germans Trias i Pujol (HGTiP), Badalona, Spain 3 Thermo Fisher Scientific, Eugene, Oregon, USA Correspondence to: Jordi Petriz, email: jpetriz@carrerasresearch.org Keywords: alkaline phosphatase; acute myeloid leukemia; stem cells; leukemic stem cells; CD34 Received: August 20, 2019 Accepted: November 13, 2019 Published: December 10, 2019 ABSTRACT In this prospective hospital-based cohort study that included 43 newly diagnosed patients with acute myeloid leukemia, flow cytometric cellular alkaline phosphatase (ALP) activity within primitive leukemic cells allowed us to identify two groups of patients at diagnosis according to the numbers of leukemic blasts expressing ≥ 12% of ALP+ cells (27 patients, Group A) and less than 12% of ALP+ cells (16 patients, Group B). Differences in outcome for complete response, relapse or treatment resistance, and exitus were statistically analyzed and were significant, when comparing the two groups. The overall survival (OS) and event-free survival (EFS) differences between Group A and B were statistically significant. The survival of Group A patients was significantly shorter than those for Group B. No significant relationship was detected in outcome when comparing ELN prognostic-risk group based on cytogenetic and molecular profile (patients in the favorable, intermediate, and adverse risk groups). Flow cytometric cellular ALP activity at diagnosis may be used to estimate relapses and disease persistence more accurately. The limitations of our study include the small number of patients enrolled and a short follow-up, due to its prospective nature.

Published
2019

42. La citometria amb enfocament acústic permet el diagnòstic de patologies relacionades amb els glòbuls vermells

Author

Petriz, Jordi

Abstract

L'estructura habitual dels eritròcits és un disc bicòncau. Moltes patologies són generades per una distorsió en aquesta estructura per diferents causes com un defecte en l'arquitectura de la membrana. La citometria amb enfocament acústic permet l'avaluació de partícules no esfèriques. El grup de Citòmica Funcional a l'Institut de Recerca de Leucèmia Josep Carreras junt amb altres grups ha estudiat aquest avantatge i ha demostrat la seva utilitat per avaluar les diferents causes de les distorsions o la qualitat dels eritròcits després de la donació de sang de manera immediata. La estructura habitual de los eritrocitos es un disco cóncavo. Sin embargo, muchas patologías son generadas por una distorsión en esta estructura por diferentes causas como por ejemplo un defecto en la arquitectura de la membrana. La citometría con enfoque acústico permite la evaluación de partículas no esféricas. Por esta razón, el grupo de Citómica Funcional a l'Institut de Recerca de Leucèmia Josep Carreras en colaboración con otros grupos ha estudiado esta ventaja de la citometría acústica y ha demostrado su utilidad para evaluar las diferentes causas que pueden provocar distorsiones a los glóbulos rojos o la calidad de los eritrocitos después de la donación de sangre de manera inmediata. The normal shape for a red blood cell is a bi-concave disc. However, a lot of pathologies are generated by a distortion in this shape due to different causes like a defects in the cell membrane skeletal architecture. Acoustic cytometry allows the study of the orientation of non-spherical particles. That is why the Citómica Funcional group in l'Institut de Recerca de Leucèmia Josep Carreras in collaboration with other groups has studied this advantage and has proven its utility to evaluate the different causes of the red blood cells distortions or to evaluate immediately the quality of erythrocytes following blood donation.

Published
2019

45. Diagnostic performance of the ClearLLab 10C B cell tube.

Author

Espasa, Andrea, Torrents, Silvia, Morales‐Indiano, Cristian, Rico, Laura G., Bardina, Jorge, Ancochea, Agueda, Bistué‐Rovira, Àngel, Linio, Rosa, Raya, Minerva, Vergara, Sara, Juncà, Jordi, Grifols, Joan‐Ramon, Petriz, Jordi, Soria, Maria‐Gloria, and Sorigue, Marc

Published
2021
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48. A Novel Flow Cytometric Method to Study Cytotoxic Activity in Whole Blood Samples.

Author

Rico, Laura G., Ward, Michael D., Bradford, Jolene A., and Petriz, Jordi

Abstract

For several decades, cell‐mediated cytotoxicity has been measured using the 51Cr release assay. This assay, however, has several drawbacks and flow cytometry has been used as an alternative to measure cytotoxic activity. Here, we present a quantitative method for cell‐mediated cytotoxicity studies, preserving cellular function with minimal sample manipulation. Cytotoxic activity is simply and reproducibly measured as the ability of cytotoxic cells to lyse K562 target cells previously loaded with Calcein‐AM vital stain. After spiking a known number of fluorescent viable K562 target cells into whole blood, cell mixtures are incubated for 2 h in a cell incubator and the remaining spiked cells are counted by flow cytometry. In order to discriminate nucleated cells, erythrocytes, and debris, unlysed whole blood is stained with a cell permeable DNA vital fluorescent dye. Cell‐mediated lysis is measured by comparing target counts for different effector‐to‐target ratios. Since the cytotoxicity of these dyes is relatively low, this method can be broadly applied to studies of innate immune response to tumors and infections, especially where target‐killing activity might be compromised by small volume samples or low frequency of cytotoxic cells. © 2020 International Society for Advancement of Cytometry [ABSTRACT FROM AUTHOR]

Published
2021
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