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openaire: EC/H2020/771049/EU//FREEDLES Funding Information: We thank Lucie Homolová, Mihkel Kaha, Bijay Karki, Lauri Korhonen, Mait Lang, Petr Lukeš, Titta Majasalmi, Jan Pisek, Ville Ranta, Daniel Schraik and the staff of Hyytiälä forestry field station for assistance in the field campaigns and data processing during 2018 – 2020. This study has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No 771049). The text reflects only the authors' view, and the Agency is not responsible for any use that may be made of the information it contains. This study was also partly funded by the Academy of Finland grant DIMEBO (323004). Publisher Copyright: © 2023 The Author(s) Remote sensing using spectral data has been commonly applied to retrieve tree layer properties while the monitoring of forest floor remains a less studied topic. We investigated the links between light availability at forest floor, and forest floor's spectral reflectance properties (350–2500 nm) and fractional cover across boreal and temperate Europe. We hypothesized that tree canopy structure (and thus, light availability at forest floor) is linked not only to the vegetation composition of forest floor, as has been shown previously, but also to forest floor's spectral reflectance properties, and that these relationships differ between forest biomes. Data were collected in situ from a total of 67 forest stands in southern boreal, hemiboreal, temperate floodplain, and temperate mountain sites. The variation of light availability at forest floor was linked to both the forest floor's composition and spectral reflectance properties. Each study site exhibited site-specific spectral features and a different mean reflectance spectrum. Openness in tree canopies was related to an increase in the fractional cover of vascular plants and to a decrease of plant litter, consequently enhancing the forest floors’ spectral absorptance features in the red and shortwave-infrared wavelengths, as well as reflectance in the near-infrared region. Also, the variations of normalized difference index values and red edge positions as functions light availability at forest floor and forest floor's composition were different for each site. Our results suggest that incorporating biome-specific relationships between tree canopy structure and forest floor reflectance properties would improve interpretation of optical remote sensing data. The measurement data are openly available.

Background Sleep health (SH) is considered a key determinant of human physiological and psychological well-being. In line with this, previous studies have found that poor sleep is associated with various psychiatric disorders, in particular, with anxiety and depression. Although little is known about the neural mechanisms underlying these associations, recent findings suggest that essential dimensions of SH are associated with altered amygdala reactivity (AR); however, evidence to date is inconsistent and reliant on small sample sizes. Methods To address this problem, the current preregistered study investigated associations between SH and AR to negative facial expressions in the UK Biobank cohort (25,758 participants). Drawing on a large sample size and consistent data acquisition, 5 dimensions of SH (insomnia symptoms, sleep duration, daytime sleepiness, chronotype, and sleep medication) were examined. Results Exploratory analyses revealed that short sleep duration was associated with decreased AR. The remaining SH dimensions and a composite measure of all SH dimensions were not associated with AR. Conclusions To our knowledge, this is the largest study to test associations between SH and AR. Habitual short sleep duration may be associated with decreased AR, possibly indicating compensation for impaired prefrontal processes and hampered emotion regulation.

Background While laparoscopic approach for right hemicolectomy (LRH) is considered appropriate for the surgical treatment of both malignant and benign diseases of right colon, there is still debate about how to perform the ileo-colic anastomosis. The ColonDxItalianGroup (CoDIG) was designed as a cohort, observational, prospective, multi-center national study with the aims of evaluating the surgeons’ attitude regarding the intracorporeal (ICA) or extra-corporeal (ECA) anastomotic technique and the related surgical outcomes. Methods One hundred and twenty-five Surgical Units experienced in colorectal and advanced laparoscopic surgery were invited and 85 of them joined the study. Each center was asked not to change its surgical habits. Data about demographic characteristics, surgical technique and postoperative outcomes were collected through the official SICE website database. One thousand two hundred and twenty-five patients were enrolled between March 2018 and September 2018. Results ICA was performed in 70.4% of cases, ECA in 29.6%. Isoperistaltic anastomosis was completed in 85.6%, stapled in 87.9%. Hand-sewn enterotomy closure was adopted in 86%. Postoperative complications were reported in 35.4% for ICA and 50.7% for ECA; no significant difference was found according to patients’ characteristics and technologies used. Median hospital stay was significantly shorter for ICA (7.3 vs. 9 POD). Postoperative pain in patients not prescribed opioids was significantly lower in ICA group. Conclusions In our survey, a side-to-side isoperistaltic stapled ICA with hand-sewn enterotomy closure is the most frequently adopted technique to perform ileo-colic anastomosis after any indications for elective LRH. According to literature, our study confirmed better short-term outcomes for ICA, with reduction of hospital stay and postoperative pain. Trial registration Clinical trial (Identifier: NCT03934151).

Background: Compliance to adjuvant chemotherapy (AC) for patients undergoing rectal surgery ranges from 43% to 73.6%. Reasons reported for not initiating or completing AC include onset of postoperative complications, drug toxicity, disease progression and/or patient preferences. Little is known regarding the impact of obesity on the compliance to AC in this setting. Methods: This multicenter, retrospective study analyzed compliance to AC and treatment-related morbidity in 511 patients having undergone surgery with curative intent for rectal cancer in six Italian colorectal centers between January 2013 and December 2017. Results:70 patients were obese (BMI > 30 kg/m2). The proportion of open procedures (22.9% vs. 13.4%) and conversions (14.3% vs. 4.8%) was greater in obese compared to non-obese patients (p

openaire: EC/H2020/771049/EU//FREEDLES Funding Information: We thank Aarne Hovi, Ville Ranta, and Daniel Schraik for collaboration. This work was supported by the Academy of Finland [BOREALITY, grant number 286390 ; and DIMEBO , grant number 3323004 ]; and by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [grant agreement No 771049 ]. The article reflects only the authors’ view and the Agency is not responsible for any use that may be made of the information it contains. Funding Information: We thank Aarne Hovi, Ville Ranta, and Daniel Schraik for collaboration. This work was supported by the Academy of Finland [BOREALITY, grant number 286390; and DIMEBO, grant number 3323004]; and by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [grant agreement No 771049]. The article reflects only the authors? view and the Agency is not responsible for any use that may be made of the information it contains. Publisher Copyright: © 2021 Modern satellite and airborne optical images have increasingly higher resolutions and enable the study of all layers of forests, not just the forest canopy. To understand the contribution of different types of understory on the overall spectral reflectance signal, ground reference data are needed from different types of forests. In this paper, we present the analysis of spectral reflectance factors (350-2300 nm) and fractional covers of understory from 36 boreal forest stands. The data were collected during peak growing season in a southern boreal forest area in Finland. The study stands represent four different forest site fertility types. We used a spectrometer to measure understory spectra in nadir and vegetation quadrats to estimate fractional cover. We showed that the understory has specific spectral features related to the site fertility type and fractional cover. Our results suggest that remote sensing can be used to differentiate forest site fertility types and estimate understory green fractional cover in northern European boreal forests. The collected data are openly available in an open data repository.

openaire: EC/H2020/771049/EU//FREEDLES This article describes a dataset of multiangular scattering properties of small trees (height = 0.38–0.7 m) at visible, near-infrared, and shortwave-infrared wavelengths (350–2500 nm), and provides supporting auxiliary data that comprise leaf, needle, and bark spectra, and structural characteristics of the trees. Multiangular spectra were measured for 18 trees belonging to three common European tree species: Scots pine (Pinus sylvestris L.), Norway spruce (Picea abies (L.) H. Karst), and sessile oak (Quercus petraea (Matt.) Liebl.). The measurements were performed in 47 different view angles across a hemisphere, using a laboratory goniometer and a non-imaging spectrometer. Leaf and needle spectra were measured for each tree, using a non-imaging spectrometer coupled to an integrating sphere. Bark spectra were measured for one sample tree per species. In addition, leaf and needle fresh mass, surface area of leaves, needles, and woody parts, silhouette area, and spherically averaged silhouette to total area ratio (STAR) for each tree were measured or derived from the measurements. The data are useful for modeling the shortwave reflectance characteristics of small trees and potentially forests, and thus benefit climate modeling or interpretation of remote sensing data.

Background: The management of rectal cancer is multimodal and involves a multidisciplinary team of cancer specialists with expertise in medical oncology, surgical oncology, radiation oncology and radiology. It is crucial for highly specialized centers to collaborate via networks that aim to maintain uniformity in every aspect of treatment and rigorously gather patients’ data, from the first clinical evaluation to the last follow-up visit. The Advanced International Mini-Invasive Surgery (AIMS) academy clinical research network aims to create a rectal cancer registry. This will prospectively collect the data of patients operated on for non-metastatic rectal cancer in high volume colorectal surgical units through a well design pre-fashioned database for non-metastatic rectal cancer, in order to take all multidisciplinary aspects into consideration. Methods/Design: The protocol describes a multicenter prospective observational cohort study, investigating demographics, frailty, cancer-related features, surgical and radiological parameters, and oncological outcomes among patients with non-metastatic rectal cancer who are candidates for surgery with curative intent. Patients enrolled in the present registry will be followed up for 5 years after surgery. Discussion: Standardization and centralization of data collection for neoplastic diseases is a virtuous process for patient care. The creation of a register will allow the control of the quality of treatments provided and permit prospective and retrospective studies to be carried out on complete and reliable high quality data. Establishing data collection in a prospective and systematic fashion is the only possibility to preserve the enormous resource that each patient represents.

openaire: EC/H2020/771049/EU//FREEDLES Understanding the reflectance anisotropy of forests and the underlying scattering mechanisms is needed to improve the accuracy of retrievals of fundamental forest characteristics from optical remote sensing data. In this paper, we developed a laboratory measurement set-up for a large goniometer (LAGOS) and measured multi-angular spectra (350–2500 nm) of 18 small trees, composed of three common European tree species: Scots pine (Pinus sylvestris L.), Norway spruce (Picea abies (L.) H. Karst), sessile oak (Quercus petraea (Matt.) Liebl.). For all trees, we measured tree spectra in 47 view angles in the upper hemisphere. To our knowledge, this is the first study reporting multi-angular reflectance spectra of single trees. We also measured the reflectance and transmittance spectra of needles and leaves, as well as reflectance spectra of bark of the sample trees. We analyzed the spectro-directional characteristics of the trees, and the inter- and intraspecific variations of these characteristics. The anisotropy oftrees was shown to be strongly asymmetrical and characteristic to species: while pine and spruce exhibited strong hotspot effects, oak showed a strong specular component. Our results indicate that simultaneous measurements of both spectral and directional characteristics of trees may enhance the discrimination of species and thus, support the retrieval of information of their biophysical properties.

Targeting relapsed acute myeloid leukemia (AML) with engineered T cells holds exceptional promise, but obstacles to efficacy exist. Understanding resistance mechanisms is essential to improve therapy and may broadly inform cancer immune escape. We and others have developed transgenic T cell receptor (TCR) therapies targeting the intracellular WT1 oncoprotein that promotes growth of most high-risk AMLs. TCR therapies generally target epitopes processed by the proteasome for Class I presentation. As mutation or loss of WT1 or loss of HLA expression is infrequent in AML, we hypothesized resistant clones may have defective antigen processing. A 25-year-old man with AML recurrent after a 2nd allogeneic stem cell transplant received reinduction chemotherapy and transgenic donor-derived T cells recognizing the HLA-A2 restricted WT1126-134 epitope (NCT01640301). The patient experienced a 12-month remission followed by relapse despite robust T cell persistence (>6% of CD8+ T cells). The relapsed leukemia expressed WT1 and HLA-A2 but was refractory to retreatment with additional WT1126-134-restricted T cells. Single cell RNA sequencing (scRNAseq) was performed on cryopreserved PBMCs from remission (n=4976 cells) and relapsed (n=2780) time points (10x Genomics platform). During apparent remission, transgenic T cells had a transcriptome distinct from native CD8s, expressing activation markers including HLA-DR, IL32, CD69 and CTLA4, suggesting recent TCR triggering from antigen encounter. However, at the time AML progression was observed, transgenic cells clustered with native quiescent CD8s, implying they were no longer encountering the targeted epitope. Transcriptome analysis of relapsed AML demonstrated downregulation of a single subunit of the immune proteasome (β1i), which we recently demonstrated in cell lines to be critical for processing the targeted WT1126-134 epitope. This patient's refractory AML was killed by T cells restricted to an alternate HLA-A2 epitope of WT1 processed independently of β1i, confirming the functional impact of immune proteasome downregulation. At an early responding timepoint, scRNAseq detected clinically unrecognized rare circulating AML cells already β1i-low, suggesting β1i loss represented immunoediting/outgrowth of a pre-existing clone as a consequence of T cell immunotherapy pressure. ScRNAseq is poised to become an important diagnostic in immunotherapy, with sufficient depth to profile both transgenic T cells and low burden/minimal residual AML, and, in our case, successfully identified a potentially targetable mechanism of immune escape. Our data suggest T cell therapy in AML and other tumors may be improved by in depth analysis of individual leukemic cells, and from selecting target epitopes processed by both standard and immunoproteasomes and/or early combination therapy combining TCRs recognizing differentially processed epitopes. Citation Format: Kelly G. Paulson, Thomas M. Schmitt, Daniel Egan, Valentin Voillet, Miranda C. Lahman, Felecia D. Wagener, Daniel S. Hunter, Petri R. Muhlhauser, Paul C. Hendrie, Cecilia Yeung, Nathalie Vigneron, Benoit Van den Eynde, Jason H. Bielas, Merav Bar, Raphael Gottardo, Aude G. Chapuis, Philip D. Greenberg. Single cell RNA sequencing reveals AML immunoediting under pressure from engineered T cell therapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 2980.

Leaf reflectance and transmittance spectra are urgently needed in interpretation of remote sensing data and modeling energy budgets of vegetation. The measurement methods should be fast to operate and preferably portable to enable quick collection of spectral databases and in situ measurements. At the same time, the collected spectra must be comparable across measurement campaigns. We compared three different methods for acquiring leaf reflectance and transmittance spectra. These were a single integrating sphere (ASD RTS-3ZC), a small double integrating sphere (Ocean Optics SpectroClip-TR), and a leaf clip (PP Systems UNI501 Mini Leaf Clip). With all methods, an ASD FieldSpec 4 spectrometer was used to measure white paper and tree leaves. Single and double integrating spheres showed comparable within-method variability in the measurements. Variability with leaf clip was slightly higher. The systematic difference in mean reflectance spectra between single and double integrating spheres was only minor (average relative difference of 1%), whereas a large difference (14%) was observed in transmittance. Reflectance measured with leaf clip was on average 14% higher compared to single integrating sphere. The differences between methods influenced also spectral vegetation indices calculated from the spectra, particularly those that were designed to track small changes in spectra. Measurements with double integrating sphere were four, and with leaf clip six times as fast as with single integrating sphere, if slightly reduced signal level (integration time reduced from optimum) was allowed for the double integrating sphere. Thus, these methods are fast alternatives to a conventional single integrating sphere. However, because the differences between methods depended on the measured target and wavelength, care must be taken when comparing the leaf spectra acquired with different methods.