1. Mind the leaf anatomy while taking ground truth with portable chlorophyll meters
- Author
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Zuzana Lhotáková, Eva Neuwirthová, Markéta Potůčková, Lucie Červená, Lena Hunt, Lucie Kupková, Petr Lukeš, Petya Campbell, and Jana Albrechtová
- Subjects
Chlorophyll ,Leaf structure ,Leaf pigments ,Leaf with hypodermis ,Vegetation index ,Remote sensing ,Medicine ,Science - Abstract
Abstract A wide range of portable chlorophyll meters are increasingly being used to measure leaf chlorophyll content as an indicator of plant performance, providing reference data for remote sensing studies. We tested the effect of leaf anatomy on the relationship between optical assessments of chlorophyll (Chl) against biochemically determined Chl content as a reference. Optical Chl assessments included measurements taken by four chlorophyll meters: three transmittance-based (SPAD-502, Dualex-4 Scientific, and MultispeQ 2.0), one fluorescence-based (CCM-300), and vegetation indices calculated from the 400–2500 nm leaf reflectance acquired using an ASD FieldSpec and a contact plant probe. Three leaf types with different anatomy were included: dorsiventral laminar leaves, grass leaves, and needles. On laminar leaves, all instruments performed well for chlorophyll content estimation (R2 > 0.80, nRMSE 0.90, nRMSE
- Published
- 2025
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