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Liquid chromatography-high-resolution mass spectrometry (LC-HRMS), as applied to untargeted metabolomics, enables the simultaneous detection of thousands of small molecules, generating complex datasets. Alignment is a crucial step in data processing pipelines, whereby LC-MS features derived from common ions are assembled into a unified matrix amenable to further analysis. Variability in the analytical factors that influence liquid chromatography separations complicates data alignment. This is prominent when aligning data acquired in different laboratories, generated using non-identical instruments, or between batches from large-scale studies. Previously, we developed metabCombiner for aligning disparately acquired LC-MS metabolomics datasets. Here, we report significant upgrades to metabCombiner that enable the stepwise alignment of multiple untargeted LC-MS metabolomics datasets, facilitating inter-laboratory reproducibility studies. To accomplish this, a "primary" feature list is used as a template for matching compounds in "target" feature lists. We demonstrate this workflow by aligning four lipidomics datasets from core laboratories generated using each institution's in-house LC-MS instrumentation and methods. We also introduce batchCombine, an application of the metabCombiner framework for aligning experiments composed of multiple batches. metabCombiner is available as an R package on Github and Bioconductor, along with a new online version implemented as an R Shiny App.

This paper describes the design, implementation and research of the Cyber Sleuth Science Lab (CSSL), an innovative educational program and supporting virtual learning environment, that combines pedagogical theory, gender inclusive instruction strategies, scientific principles/practices, gamification methods, computational thinking, and real-world problem solving. This program provides underrepresented youth, especially girls, with digital forensic knowledge, skills and career pathways, challenging them to explore complex social issues related to technology and to become cyber sleuths using real-world digital forensic methods and tools to solve investigative scenarios. Students also learn about related careers while improving their cyber street smarts. The CSSL incorporates additional "outside of the computer" activities to strengthen students' engagement such as structured in-classroom discussions, mock trials, and in-person interactions with practitioner role models. The CSSL was piloted in various forms to assess the suitability for in-school and out-of-school settings, and the students predominantly represented racial minorities. Research in this project relied on a mixed methods approach for data collection and analysis, including qualitative and quantitative methods, reinforced using learning analytics generated from the students clicking through the interface and interacting with the system. Analysis of gathered data indicate that the virtual learning environment developed in this project is highly effective for teaching digital forensic knowledge, skills, and abilities that are directly applicable in the workplace. Furthermore, the strategies for gender inclusive STEM instruction implemented in CSSL are effective for engaging girls without being harmful to boys' engagement. Learning STEM through digital forensic science taps into girls' motivations to address real-world problems that have direct relevance to their lives, and to protect and serve their community. After participating in the educational program, girls expressed a significantly greater increase in interest, relative to boys, in learning more about careers related to digital forensics and cybersecurity.

Background/objectivesLittle is known about the separate or combined effects of state and national nutrition policies regulating food and beverages in schools on child overweight/obesity (OV/OB) and related racial/ethnic disparities. We investigated the influence of school nutrition policies enacted in California, independently and in combination with the United States' national policy "Healthy Hunger Free Kids Act" (HHFKA) on childhood OV/OB and racial/ethnic disparities.Subjects/methodsAn interrupted time series design was used with data from 12,363,089 child-level records on 5th- and 7th-graders in California public schools to estimate sex- and racial/ethnic-specific time trends in OV/OB prevalence during three periods: before the California nutrition policies (2002-2004); when only California policies were in effect (2005-2012); and when they were in effect simultaneously with HHFKA (2013-2016).ResultsBefore the state's policies, OV/OB prevalence increased annually among children in most subgroups. Improvements in OV/OB trends were observed for almost all groups after the California policies were in effect, with further improvements after the addition of HFFKA. The total change in annual log-odds of OV/OB, comparing the periods with both state and federal policies versus no policies, ranged from -0.08 to -0.01 and varied by grade, sex, and race/ethnicity. Within each sex and grade, the greatest changes were among African-American (-0.08 to -0.02, all p

In scientific disciplines, most postdoctoral fellowships focus on research training. Postdoctoral fellows ("postdocs") develop research expertise and research projects that they will use in future independent faculty positions. This research focus often precludes opportunities for undergraduate teaching. However, most academic faculty positions require faculty to teach at the undergraduate level. The result is that many postdocs are exceptionally well-qualified to meet the research expectations of future faculty positions, but lack experience and training in innovative and evidence-based undergraduate teaching strategies. Training in evidence-based teaching approaches can result in two tangible outcomes. First, the quality of applications by the postdocs for tenure-track faculty positions at institutions with substantive teaching expectations can be improved. Second, we can anticipate stronger alignment of teaching and learning expectations between new faculty and their undergraduate students. There are many programs that provide training in teaching to early career researchers. We describe the design and implementation of a mentored teaching experience that faced some unique challenges, including a large geographic distance between the postdocs and the teaching mentor and teaching site. We describe how we addressed the challenges, what the benefits to various stakeholders have been, and the key elements that contributed to the success of the program.

Following a 2019 workshop led by the Center for Global Health Studies at the Fogarty International Center on the topic of childhood obesity prevention and research synergies transpiring from cross-border collaborations, we convened a group of experts in the United States and Latin America to conduct a narrative review of the epidemiological literature on the role of obesogenic endocrine disrupting chemicals (EDCs) in the etiology of childhood obesity among Latino youth in the United States and Latin America. In addition to summarizing and synthesizing results from research on this topic published within the last decade, we place the findings within a lifecourse biobehavioral framework to aid in identification of unique exposure-outcome relationships driven by both biological and behavioral research, identify inconsistencies and deficiencies in current literature, and discuss the role of policy regulations, all with the goal of identifying viable avenues for prevention of early life obesity in Latino/Hispanic populations.

International migration has economic and health implications. The acculturation process to the host country may be linked to childhood obesity. We use the Community Energy Balance (CEB) framework to analyze the relationship between migration and childhood obesity in Mexican households with international migrants. Using longitudinal data from the Mexican Family Life Survey (MxFLS), we examine how migrant networks affect childhood obesity in origin communities. We also review binational health programs that could be effective at tackling childhood obesity in migrant households from Mexico. Children embedded in migrant networks are at greater risk of developing overweight or obesity, suggesting a significant relationship between childhood obesity and international migration in Mexican households. Based on our search criteria, our analysis of health outreach programs shows that Ventanillas de Salud (VDS)/Health Windows has great promise to prevent childhood obesity in a culturally sensitive and trustful environment. The CEB framework is useful to understand how migration contributes to the risk of childhood overweight and obesity in migrant households. VDS is a feasible and replicable strategy with great potential to address childhood obesity among migrant families accounting for the dynamic and binational determinants of childhood obesity.

International migration has economic and health implications. The acculturation process to the host country may be linked to childhood obesity. We use the Community Energy Balance (CEB) framework to analyze the relationship between migration and childhood obesity in Mexican households with international migrants. Using longitudinal data from the Mexican Family Life Survey (MxFLS), we examine how migrant networks affect childhood obesity in origin communities. We also review binational health programs that could be effective at tackling childhood obesity in migrant households from Mexico. Children embedded in migrant networks are at greater risk of developing overweight or obesity, suggesting a significant relationship between childhood obesity and international migration in Mexican households. Based on our search criteria, our analysis of health outreach programs shows that Ventanillas de Salud (VDS)/Health Windows has great promise to prevent childhood obesity in a culturally sensitive and trustful environment. The CEB framework is useful to understand how migration contributes to the risk of childhood overweight and obesity in migrant households. VDS is a feasible and replicable strategy with great potential to address childhood obesity among migrant families accounting for the dynamic and binational determinants of childhood obesity.

Introduction: Heavy metals such as Lead (Pb) and Mercury (Hg) can affect adipose tissue mass and function. Considering the high prevalence of exposure to heavy metals and obesity in Mexico, we aim to examine if exposure to Pb and Hg in adolescence can modify how fat is accumulated in early adulthood. Methods: This study included 100 participants from the ELEMENT cohort in Mexico. Adolescent Pb and Hg blood levels were determined at 14-16 years. Age- and sex-specific adolescent BMI Z-scores were calculated. At early adulthood (21-22 years), fat accumulation measurements were performed (abdominal, subcutaneous, visceral, hepatic, and pancreatic fat). Linear regression models with an interaction between adolescent BMI Z-score and Pb or Hg levels were run for each adulthood fat accumulation outcome with normal BMI as reference. Results: In adolescents with obesity compared to normal BMI, as Pb exposure increased, subcutaneous (p-interaction = 0.088) and visceral (p-interaction < 0.0001) fat accumulation increases. Meanwhile, Hg was associated with subcutaneous (p-interaction = 0.027) and abdominal (p-interaction = 0.022) fat deposition among adolescents with obesity. Conclusions: Heavy metal exposure in adolescence may alter how fat is accumulated in later periods of life.

Purpose: Little is known regarding women's lived experiences of how diet impacts sleep. Based on ethnographic interviews among working-class women from Mexico City, our primary aim was to identify themes related to diet and sleep among midlife women. Informed by qualitative analyses, a secondary aim was to examine associations between tea and sleep duration in a broader cohort. Materials and methods: We conducted a cross-sectional study that entailed in-depth ethnographic interviews about sleep and other behaviors, including diet, with a purposive sample of 30 women from the ELEMENT cohort. Ethnographer field notes and transcripts were analyzed using thematic analysis. Guided by findings from the interviews demonstrating that tea consumption might be associated with sleep, we conducted post-hoc analyses of the relationship between tea and sleep duration using data from food frequency questionnaires and actigraphy, respectively, in the broader cohort (n = 406). Results: The mean (SD) age of the ethnographic sample was 50.0 (9.0) years. The top noted theme was the use of herbal tea (in Spanish infusion) to improve sleep; most women (29/30) discussed herbal teas, characterizing them as a "natural remedy" to facilitate sleep. The mean (SD) age of the broader sample (N = 406) was 48.4 (6.2) years. Post-hoc analyses revealed positive associations between tea without sugar (though not necessarily herbal tea) and sleep duration. We found that every serving of tea without sugar consumed was associated with an 18.0 min per night [β (SE) = 18.0 (7.8); p = 0.022] and a 13.4 min per night [β (SE) =13.4 (5.6); p = 0.017] increase in weekend and 7-day sleep duration, respectively. Conclusions: Within a sample of 30 midlife women, dietary practices were described in relation to sleep, specifically the consumption of herbal teas to promote sleep. [ABSTRACT FROM AUTHOR]

Background: Aboriginal and Torres Strait Islander youth are vulnerable to racism, trauma and Lateral Violence (LV) where negative feelings and behaviours are directed towards members within their own oppressed group. Conversely, Lateral Empowerment (LE) is the collective prevention and repair of the effects of LV and promotes resilience and strength. There is limited peer reviewed literature directly relating to LV and LE. Objective: This review focuses on grey literature to gain greater insight into the understanding and experiences of LV/LE among Aboriginal and Torres Strait Islander young people within Australia. Method: This grey literature scoping review identified N = 38 documents between January 1980 and September 2023 related to LV or LE to gain a greater insight into the understanding of LV and LE among the Aboriginal and Torres Strait Islander community outside of published publications. Results: The results elucidated that the experience of LV for Aboriginal and Torres Strait Islander youth is largely based upon internalised racism pertaining to Aboriginal identity. Strength-based gender-specific approaches which focus upon positive cultural experiences, the use of lived experience mentors and the inclusion of family were identified as the foundation for LE. Conclusion: The grey literature review highlights that the Aboriginal and Torres Strait Islander community are taking an active role in addressing and preventing LV through culturally informed practices and approaches based upon the truth telling of Australia's colonial history. There remains the need for specific approaches directed at Aboriginal and Torres Strait Islander youth to help prevent and address LV/LE. KEY POINTS: What is already known about this topic: LV is an emerging area of discourse within Aboriginal and Torres Strait Islander Communities. LV is prevalent within Aboriginal and Torres Strait Islander communities as a symptom of the ongoing oppression of colonisation, with young people particularly vulnerable and most at risk. LE is a relatively new term that aims to counteract LV by empowering Aboriginal and Torres Strait Islander people to have control over their lives. What the topic adds: This analysis provides a thorough review of unpublished information ("grey literature") pertaining to LV and LE for Aboriginal and Torres Strait Islander people within Australia, with a specific focus on young people. Insight into the voices of Aboriginal and Torres Strait Islander young people and their communities in relation to their understanding and lived experiences of LV/LE. Insight into the experiences of Aboriginal and Torres Strait Islander youth and their communities with regard to approaches to counteract the effects of LV. [ABSTRACT FROM AUTHOR]

Background/Objectives: This study aimed to examine associations between meal timing habits and sleep health in midlife Mexican women. Methods: Data comprised 379 midlife Mexican women who participated in a phone survey conducted within the Early Life Exposures in Mexico to Environmental Toxicants (ELEMENT) project during the early stages of the COVID-19 pandemic. Women answered questions related to meal habits and sleep duration, latency, and quality. We used linear regression to investigate the associations between meal timing, frequency of meals/snacks, eating window (duration between first and last eating occasion of the day), duration between last meal, bedtime, sleep duration, and logistic regression to examine the associations between meal timing, sleep latency, and sleep quality, adjusting for confounders. Results: Later timing of meals throughout the day, and a shorter interval between the last meal of the day and bedtime, were associated with prolonged sleep latency and worse sleep quality. Associations with sleep duration were mixed: a longer eating window and a later largest and last meal were each associated with shorter sleep duration, while a later first meal and a shorter interval between the last meal of the day and bedtime were associated with longer sleep duration. Conclusions: Meal timing habits are associated with sleep duration, latency, and quality in midlife women. [ABSTRACT FROM AUTHOR]

Acquiescence is often defined as the systematic selection of agreeable ("strongly agree") or affirmative ("yes") responses to survey items, regardless of item content or directionality. This definition implies that acquiescence is immune to item characteristics; however, the influence of item characteristics on acquiescence remains largely unexplored. We examined the influence of eight item characteristics on acquiescence in a telephone survey of 400 Latinos and non-Latino Whites: qualified wording, mental comparisons, negated wording, unfamiliar terms, ambiguous wording, knowledge accessibility, item length, and polysyllabic wording. Negated and ambiguous wording was associated with reduced acquiescence for the full sample as well as subsamples stratified by ethnicity and sociodemographic characteristics. This effect was strongest among younger, more educated, and non-Latino white respondents. No other item characteristics had a significant influence on respondent acquiescence. Findings from this study suggest that acquiescence may be affected by interactions between respondent and item characteristics.