1. The gene encoding the late nonstructural 36K protein of vaccinia virus is essential for virus reproduction
- Author
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O. I. Ryazankina, Sergei N. Shchelkunov, and Peter V. Gashnikov
- Subjects
Cancer Research ,Virus Integration ,viruses ,Vaccinia virus ,Genome, Viral ,Viral Nonstructural Proteins ,Biology ,Virus Replication ,medicine.disease_cause ,Recombinant virus ,Virus ,Viral Proteins ,chemistry.chemical_compound ,Virology ,Chlorocebus aethiops ,medicine ,Animals ,Poxviridae ,Orthopoxvirus ,Gene ,Cells, Cultured ,Recombination, Genetic ,biology.organism_classification ,Molecular biology ,Rats ,Phenotype ,Infectious Diseases ,Herpes simplex virus ,chemistry ,DNA, Viral ,Vaccinia ,Plasmids - Abstract
Two genetic markers--the thymidine kinase gene of herpes simplex virus, and the beta-galactosidase gene of Escherichia coli--were incorporated into the 36K protein gene (IL1 gene according to the nomenclature of the Copenhagen strain of vaccinia virus; Goebel et al., 1990) from the HindIII-P DNA fragment of the LIVP strain (variant of Lister strain) of vaccinia virus (VV). After recombination of the obtained integration plasmid pVZ64-TK with the VV genome (tk-), it was found that the resultant TK+ viruses were unstable with respect to the Lac+ phenotype. On the basis of hybridization of DNA fragments of selected clones, a scheme for the formation of hybrid viruses is proposed, and an approach to a simple phenotypical discrimination between essential and non-essential genes for VV viability is described.
- Published
- 1993
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