1. Determination ofN7‐glycidamide guanine adducts in human blood DNA following exposure to dietary acrylamide using liquid chromatography/tandem mass spectrometry
- Author
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Donald J. L. Jones, Raj Singh, Victoria Emms, Peter B. Farmer, Derryn Grant, Paulene Quinn, Colleen Maxwell, Antria Mina, Leong L. Ng, Sandra Schumacher, and Robert G. Britton
- Subjects
Dietary Exposure ,Acrylamide ,DNA Adducts ,Guanine ,Tandem Mass Spectrometry ,Organic Chemistry ,Epoxy Compounds ,Humans ,DNA ,Lymphocytes ,Spectroscopy ,Chromatography, Liquid ,Analytical Chemistry - Abstract
Acrylamide is classified as a probable human carcinogen that is metabolised to glycidamide, which can covalently bind to DNA. The aim of this study was to investigate the formation of N7-glycidamide guanine (N7-GA-Gua) adducts in human blood DNA following exposure to acrylamide present in carbohydrate-rich foods as part of the normal human diet.Lymphocyte DNA was extracted from blood samples obtained from healthy human volunteers. Following thermal depurination of the DNA samples, N7-GA-Gua adducts were quantified using a validated liquid chromatography/tandem mass spectrometry (LC/MS/MS) method incorporating a stable isotope labelled internal standard. Estimated dietary acrylamide intake was recorded by completion of food frequency questionnaires for the 24 hours prior to volunteer blood donation.An LC/MS/MS method was validated with a limit of detection of 0.25 fmol and a lower limit of quantitation of 0.50 fmol on column. N7-GA-Gua adducts were detected in human blood DNA with the levels ranging between 0.3 to 6.3 adducts per 10Identification and quantification of N7-GA-Gua adducts in the blood DNA of healthy volunteers suggests that dietary acrylamide exposure may lead to the formation of DNA adducts. This important finding warrants further investigation to ascertain a correlation between environmental/dietary acrylamide exposure and levels of DNA adducts.
- Published
- 2022
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