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We hypothesized that near-future elevated CO2 would affect the antipredatory behavior of two freshwater organisms; a pulmonate gastropod (Physella columbiana) and a cladoceran crustacean (Daphnia magna). Studies have found that pCO2 and increased acidification due to CO2 impedes fright responses to predators by activating GABAA receptors. After administration of predator-derived kairomones and conspecific alarm cues, we also briefly exposed some of the animals to gabazine which is a GABAA receptor antagonist to restore a fright response. We found that added carbon dioxide negatively affected the antipredatory behavior of both species but gabazine did not reverse this effect. To further examine the effect of CO2 and gabazine, we also tested the effect of stressful crowding, cold, and acidic conditions on the production of male daphnid offspring. An increase in ratio of male to female offspring is a common and expected response to stress by daphnids. We found that stress increased the production of males and gabazine reversed this at a pH of 5.5 but not at pH 6.2 or 6.5. Our study suggest that while the main negative effects of anthropogenic CO2 enrichment can be robust, the myriad indirect effects of CO2 make predictions about future predator prey systems less clear.

Mycobacteriophages DrHayes, Urkel, and SamuelLPlaqson were isolated from soil samples in Spokane, WA, using Mycobacterium smegmatis mc 2 155 grown at room temperature. The three genomes differ by only a few nucleotides, are 60,526 bp long, have 97 predicted protein-coding genes and one tRNA gene, and are members of subcluster K1.