1. Giardia lamblia: Identification of peroxisomal-like proteins.
- Author
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Acosta-Virgen K, Chávez-Munguía B, Talamás-Lara D, Lagunes-Guillén A, Martínez-Higuera A, Lazcano A, Martínez-Palomo A, and Espinosa-Cantellano M
- Subjects
- 3,3'-Diaminobenzidine chemistry, Animals, Antibodies, Protozoan biosynthesis, Antibodies, Protozoan immunology, Blotting, Western, Cerium chemistry, Coenzyme A Ligases immunology, Coenzyme A Ligases metabolism, Computational Biology, Fluorescent Antibody Technique, Giardia lamblia enzymology, Giardia lamblia immunology, Giardia lamblia ultrastructure, Histocytochemistry, Microscopy, Confocal, Microscopy, Electron, Transmission, Microscopy, Immunoelectron, Oxidoreductases metabolism, Peroxins analysis, Peroxins immunology, Peroxisomes enzymology, Protozoan Proteins analysis, Rabbits, Staining and Labeling, Giardia lamblia chemistry, Peroxisomes chemistry, Protozoan Proteins isolation & purification
- Abstract
The protozoan parasite Giardia lamblia has traditionally been reported as lacking peroxisomes, organelles involved in fatty acid metabolism and detoxification of reactive oxygen species. We here report the finding with transmission electron microscopy of an oxidase activity in cytoplasmic vesicles of trophozoites and cysts of G. lamblia. These vesicles were positive to 3,3'-diaminobenzidine and to cerium chloride staining. In addition, using bioinformatic tools, two peroxisomal proteins were identified in the G. lamblia proteome: acyl-CoA synthetase long chain family member 4 (ACSL-4) and peroxin-4 (PEX-4). With confocal and immunoelectron microscopy using polyclonal antibodies both proteins were identified in cytoplasmic vesicles of trophozoites. Altogether, our results suggest for the first time the presence of peroxisomal-like proteins in the cytoplasm of G. lamblia., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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