7,098 results on '"Periodic acid"'
Search Results
2. Reversible O‑Acetyl Migration within the Sialic Acid Side Chain and Its Influence on Protein Recognition
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Ji, Yang, Sasmal, Aniruddha, Li, Wanqing, Oh, Lisa, Srivastava, Saurabh, Hargett, Audra A, Wasik, Brian R, Yu, Hai, Diaz, Sandra, Choudhury, Biswa, Parrish, Colin R, Freedberg, Darón I, Wang, Lee-Ping, Varki, Ajit, and Chen, Xi
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Biological Sciences ,Organic Chemistry ,Chemical Sciences ,Infectious Diseases ,Biotechnology ,Emerging Infectious Diseases ,Acetylation ,Animals ,Cattle ,Chromatography ,High Pressure Liquid ,Hemagglutinins ,Viral ,Molecular Structure ,Oxidation-Reduction ,Periodic Acid ,Phenylenediamines ,Polysaccharides ,Protein Binding ,Sialic Acids ,Torovirus ,Viral Fusion Proteins ,Biological sciences ,Chemical sciences - Abstract
O-Acetylation is a common naturally occurring modification of carbohydrates and is especially widespread in sialic acids, a family of nine-carbon acidic monosaccharides. O-Acetyl migration within the exocyclic glycerol-like side chain of mono-O-acetylated sialic acid reported previously was from the C7- to C9-hydroxyl group with or without an 8-O-acetyl intermediate, which resulted in an equilibrium that favors the formation of the 9-O-acetyl sialic acid. Herein, we provide direct experimental evidence demonstrating that O-acetyl migration is bidirectional, and the rate of equilibration is influenced predominantly by the pH of the sample. While the O-acetyl group on sialic acids and sialoglycans is stable under mildly acidic conditions (pH < 5, the rate of O-acetyl migration is extremely low), reversible O-acetyl migration is observed readily at neutral pH and becomes more significant when the pH increases to slightly basic. Sialoglycan microarray studies showed that esterase-inactivated porcine torovirus hemagglutinin-esterase bound strongly to sialoglycans containing a more stable 9-N-acetylated sialic acid analog, but these compounds were less resistant to periodate oxidation treatment compared to their 9-O-acetyl counterparts. Together with prior studies, the results support the possible influence of sialic acid O-acetylation and O-acetyl migration to host-microbe interactions and potential application of the more stable synthetic N-acetyl mimics.
- Published
- 2021
3. Periodic Acid Modification of Chemical‐Bath Deposited SnO2 Electron Transport Layers for Perovskite Solar Cells and Mini Modules.
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Wu, Ziyi, Su, Jiazheng, Chai, Nianyao, Cheng, Siyang, Wang, Xuanyu, Zhang, Ziling, Liu, Xuanling, Zhong, Han, Yang, Jianfei, Wang, Zhiping, Liu, Jianbo, Li, Xin, and Lin, Hong
- Subjects
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SOLAR cells , *CHEMICAL solution deposition , *PEROVSKITE , *ELECTRON transport , *TIN oxides , *STANNIC oxide - Abstract
Chemical bath deposition (CBD) has been demonstrated as a remarkable technology to fabricate high‐quality SnO2 electron transport layer (ETL) for large‐area perovskite solar cells (PSCs). However, surface defects always exist on the SnO2 film coated by the CBD process, impairing the devices' performance. Here, a facile periodic acid post‐treatment (PAPT) method is developed to modify the SnO2 layer. Periodic acid can react with hydroxyl groups on the surface of SnO2 films and oxidize Tin(II) oxide to Tin(IV) oxide. With the help of periodic acid, a better energy level alignment between the SnO2 and perovskite layers is achieved. In addition, the PAPT method inhibits interfacial nonradiative recombination and facilitates charge transportation. Such a multifunctional strategy enables to fabricate PSC with a champion power conversion efficiency (PCE) of 22.25%, which remains 93.32% of its initial efficiency after 3000 h without any encapsulation. Furthermore, 3 × 3 cm2 perovskite mini‐modules are presented, achieving a champion efficiency of 18.10%. All these results suggest that the PAPT method is promising for promoting the commercial application of large‐area PSCs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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4. Single-Step Regioselective Oxidative Iodination of Tetrahydrocarbazoles using Periodic Acid in Methanol.
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Naykode, M. S., Lokhande, M. N., and Lokhande, P. D.
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IODINATION , *CYCLOHEXANONES , *ACIDS , *HYDRAZINE , *HYDRAZINES - Abstract
Methanol was used as a solvent to perform a one-pot direct oxidation of tetrahydrocarbazoles with periodic acid, followed by regioselective iodination. The oxidative iodination products, substituted 3-iodo-1,2,3,4-tetrahydro-9H-carbazol-1-ones, were obtained in 60–82% yields. Initial tetrahydrocarbazole derivatives were prepared from cyclohexanone and substituted phenyl hydrazines. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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5. Evaluation of the diagnosis of helicobacter pylori from stomach biopsy samples by staining methods.
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Karaman, Ulku, Direkel, Sahin, Erdem, Havva, Kaya, Yasemin, Arici, Yeliz Kasko, and Gul, Tuba
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HELICOBACTER pylori ,HEMATOXYLIN & eosin staining ,STOMACH biopsy ,METAPLASIA ,PERIODIC acid - Abstract
In this study, it was aimed to retrospectively investigate the presence of H. pylori with Modified Giemsa and Hematoxylin & Eosin in gastric antrum biopsy samples of patients who applied to Ordu University hospital with dyspeptic complaints. Also, cross-sections were stained with Giemsa, Wright's eosin methylene blue and modified Giemsa dyes to compare their effectiveness in diagnosis. The population of the study consisted of 2679 gastric biopsy samples sent to the pathology laboratory between 2014 and 2018. Gastric biopsy samples were screened, and samples stained with Hematoxylin & Eosin, Giemsa and Periodic Acid Schift (PAS) were re-examined for H. pylori In the microscopic examination, the samples were also evaluated in terms of intestinal metaplasia, activation and atrophy. In the study, 37 negative, 31 mildly positive, 31 moderately positive and 31 severely positive samples were randomly selected, in addition to routine staining methods. Selected samples were re-sectioned, stained with Giemsa and Wright's eosin dye, and Mayg Grunwald-Giemsa (MGG) dye, Giemsa and Wright's eosin methods were compared. A total of 2679 patients, 49.15% male and 50.85% female, were included in the study. The mean age of patients aged 17-93 was 50.42±15.32. H. pylori positivity was found to be 46.8% in the study. It was determined in the study that there was a significant association between H. pylori severity and inflammation (p<0.01). The increase in H. pylori severity also increased the incidence of activation positivity. The ratio of atrophic patients (56.4%) was higher in patients with severe H. pylori positivity (43.6%). Conclusion: It was observed that the incidence of the bacterium was high in Ordu province, and it was suggested that the public should be informed about the transmission and prevention ways of H. pylori. Moreover, since similar results were obtained with the staining methods applied in the study, it was concluded that Giemsa and Wright's eosin dyes can be used because they are easy and cheap to diagnose. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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6. Lieber-DeCarli Diet Promotes Different Hepatic Histological Changes During Early and Late Pregnancy: An Example of Maternal Resilience.
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DIAZ-MUNOZ, MAURICIO, DE LOS RIOS-ARELLANO, ERICKA ALEJANDRA, CRUZ-RAMOS, JOSE A., CAMACHO-BARRON, MARIELA, LUNA-MORENO, DALIA, ANAYA-LOYOLA, MIRIAM ARACELY, SANTOYO-TELLES, FELIPE, and NAVARRO-MEZA, MONICA
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ETHANOL ,GESTATIONAL age ,LIVER cells ,PERIODIC acid ,LIVER glycogenic function - Abstract
Background/Aim: During pregnancy, maternal liver can be affected by ethanol (ETOH) intake, whose effects depend on concentration levels ingested. This study aims to describe histological and serum marker characteristics of maternal liver during two metabolic conditions: gestation (G), and sustained ETOH intake, in early and late pregnancy. Materials and Methods: Wistar rats were fed with Lieber-DeCarli diet during pregnancy, following an experimental protocol that allows a semi-chronic intake of ETOH (5%). Liver and serum samples were processed for histological characterization and biochemical profiling. Hematoxylin/eosin and Schiff's Periodic Acid staining were used. Results: During pregnancy, a significant elevation in ballooned and edamatous hepatocytes, and a significant increase in micro and macrovesicular deposits were observed in rats fed with the ETOH diet at gestation days 3G, 8G and 15G. These changes were reverted by 20G. Liver glycogen content increased significantly at 15G. Serum metabolites in pregnant rats fed with the ETOH diet showed a significant reduction in urea (from 3G to 15G), an increase in albumin and uric acid at 20G, and a reduction in creatinine. Number of offsprings and weight of male newborns were reduced by 20% and 14%, respectively. Liver function markers in serum showed no significant changes. Conclusion: ETOH diet intake promotes hepatic histological changes and histological modifications during pregnancy. These results support the assumption that pregnancy is an adaptive procedure that is associated with nutritional conditions and has a strong influence on hepatic histology. They suggest that pregnancy promotes a state of resilience to the liver function during the sustained intake of 5% ETOH. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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7. Efficacy of periodic acid–Schiff stain in the diagnosis of onychomycosis – A cross‑sectional study.
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Salahudeen, Mohamed, Ramdas, Anita, Elumalai, Arthi, Ravichandran, Kandasamy, and Kuruvila, Sheela
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ONYCHOMYCOSIS , *PERIODIC acid , *DERMATOLOGY , *TERTIARY care - Abstract
Background: Onychomycosis is the fungal infection of the nail which needs to be differentiated from other causes of dystrophic nails due to its long-term treatment. Direct microscopic examination with potassium hydroxide (KOH) mount along with fungal culture shows inconsistent sensitivity. Thus, there is a need of more accurate and efficient method to detect onychomycosis. Aims and Objectives: This study aims to compare periodic acid–Schiff (PAS) stain with KOH and nail fungal culture in the diagnosis of onychomycosis. Materials and Methods: This was a cross-sectional study conducted in the dermatology outpatient department of a tertiary care center in South India from July 2019 to July 2021. A total of 93 patients with clinical suspicion of onychomycosis were included in the study and evaluated for the following three methods: KOH mount, nail fungal culture, and PAS stain. Results: Of the 93 patients, PAS was positive in 82 (88%) cases, KOH mount was positive in 32 (34%) cases, and nail fungal culture showed positive results in 49 (53.2%) patients. The percentage of positivity of combined PAS and culture was 89 (95.7%) and combined KOH and culture was 63 (65.7%). The culture yielded growth in 49 patients showing dermatophytes in 2, yeasts in 35, and nondermatophyte molds (NDMs) in 12 specimens The most common isolated species was Candida tropicalis found in 11 (22.4%) followed by Candida species in 8 (16.3%), Candida albicans in 7 (14.2%), and Trichosporon species in 6 (12.2%) specimens. Among the NDMs, Fusarium oxysporum was the most isolated found in 5 (10.2%). Conclusion: The importance of this study is to highlight the utility of PAS stain in the diagnosis of onychomycosis which otherwise may be missed with KOH and culture. PAS can be performed as an additional test to KOH mount and nail fungal culture to close the diagnostic gap. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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8. Sustainable bioelectric activation of periodate for highly efficient micropollutant abatement.
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Zou R, Yang W, Rezaei B, Tang K, Zhang P, Andersen HR, Sylvest Keller S, and Zhang Y
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- Wastewater, Periodic Acid, Oxidation-Reduction, Carbamazepine, Hydrogen Peroxide, Water Pollutants, Chemical analysis
- Abstract
The periodate (PI)-based advanced oxidation process is valued for environmental remediation, but current activation methods involve high costs, secondary contamination risks, and limited applicability due to external energy inputs (e.g., UV), catalyst incorporation (e.g., Fe
2+ ), or environmental modifications (e.g., freezing). In this work, novel bioelectric activation of PI using the electrons generated by electroactive bacteria was developed and investigated for rapid removal of carbamazepine (CBZ), achieving 100 %, 100 %, and 76 % removal efficiency for 4.22 µM of CBZ in 20 min at pH 2, 120 min at pH 6.4, and HRT of 30 min at pH 8.5, respectively, with a 1 mM PI dose and without an input voltage. It was deduced that electrons derived from bacteria could directly activate PI using Ti mesh electrodes and generate •IO3 via single electron transfer under strongly acidic conditions (e.g., pH 2). Nevertheless, under weak alkaline conditions (e.g., pH 8.5), biogenic electrons indirectly activated PI by generating OH- via 4e- reduction at the Ti mesh cathode, resulting in the formation of •O2 - and1 O2 . In addition to the metal cathode, a carbon-based cathode finely modulates the 2e- reduction, yielding H2 O2 and activating PI to mainly form •OH. Moreover, primarily non-toxic IO3 - was produced during treatment, while no detectable reactive iodine species (HOI, I2 , and I3 - ) were observed. Furthermore, the bioelectric activation of PI demonstrated its capability to remove various micropollutants present in secondary-treated municipal wastewater, showcasing its broad-spectrum degradation ability. This study introduces a novel, cost-effective, and environmentally friendly PI activation technique with promising applicability for micropollutant elimination in water treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)- Published
- 2024
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9. Infiltration of chitin by protein coacervates defines the squid beak mechanical gradient
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Tan, YerPeng, Hoon, Shawn, Guerette, Paul A, Wei, Wei, Ghadban, Ali, Hao, Cai, Miserez, Ali, and Waite, J Herbert
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Amino Acid Sequence ,Animals ,Beak ,Biomechanical Phenomena ,Catechols ,Chitin ,Cross-Linking Reagents ,Decapodiformes ,Hardness ,Molecular Sequence Data ,Periodic Acid ,Protein Binding ,Protein Structure ,Tertiary ,Proteins ,Proteomics ,Sequence Analysis ,RNA ,Water ,Medicinal and Biomolecular Chemistry ,Biochemistry and Cell Biology ,Biochemistry & Molecular Biology - Abstract
The beak of the jumbo squid Dosidicus gigas is a fascinating example of how seamlessly nature builds with mechanically mismatched materials. A 200-fold stiffness gradient begins in the hydrated chitin of the soft beak base and gradually increases to maximum stiffness in the dehydrated distal rostrum. Here, we combined RNA-Seq and proteomics to show that the beak contains two protein families. One family consists of chitin-binding proteins (DgCBPs) that physically join chitin chains, whereas the other family comprises highly modular histidine-rich proteins (DgHBPs). We propose that DgHBPs play multiple key roles during beak bioprocessing, first by forming concentrated coacervate solutions that diffuse into the DgCBP-chitin scaffold, and second by inducing crosslinking via an abundant GHG sequence motif. These processes generate spatially controlled desolvation, resulting in the impressive biomechanical gradient. Our findings provide novel molecular-scale strategies for designing functional gradient materials.
- Published
- 2015
10. Structural characterization of a glycoprotein from white jade snails (Achatina Fulica) and its wound healing activity.
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Li Z, Song Y, Ling Y, Liu Y, Yi J, Hao L, Zhu J, Kang Q, Huang J, and Lu J
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- Animals, Humans, Mice, Spectroscopy, Fourier Transform Infrared, Periodic Acid, Wound Healing, Polysaccharides pharmacology, Polysaccharides chemistry, Glycoproteins, Snails
- Abstract
Snail mucus is rich in proteins and polysaccharides, which has been proved to promote wound healing in mice in our previous research. The aim of this study was to investigate the effective component in snail mucus that can exert the wound healing potential and its structural characterization. Here, the glycoprotein from the snail mucus (SM1S) was obtained by DEAE-Sepharose Fast Flow and Sephacryl S-300 columns. The structural characteristics of SM1S were investigated via chromatographic techniques, periodic acid oxidation, FT-IR spectroscopy and NMR spectroscopy. Results showed that SM1S was a glycoprotein with a molecular weight of 3.8 kDa (83.23 %), consists of mannose, glucuronic acid, glucose, galactose, xylose, arabinose, fucose at a ratio of 13.180:4.875:1043.173:7.552:1:3.501:2.058. In addition, the periodic acid oxidation and NMR analysis showed that SM1S contained 1,6-glycosidic bonds, and might also contain 1 → 4 and 1 → 2 glycosidic or 1 → 3 glycosidic bonds. Furthermore, the migration experiment of human skin fibroblasts in vitro suggested that SM1S had a good effect to accelerate the scratch healing of cells. This study suggested that SM1S may be a prospective candidate as a natural wound dressing for the development of snail mucus products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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11. Simulated sunlight/periodate-triggered formation of toxic halogenated bisphenols in highly saline water.
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Xie Y, Zhang K, Shen Z, Feng M, and Wang C
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- Benzhydryl Compounds chemistry, Saline Waters, Oxidation-Reduction, Sunlight, Water Pollutants, Chemical chemistry, Periodic Acid, Phenols
- Abstract
Periodate (PI)-based oxidation using the activators, such as metal ions and light irradiation, has emerged as a feasible treatment strategy for the effective remediation of contaminated water and wastewater. Given the pervasive nature of PI residues and solar exposure during application, the role of solar light in remediating the challenging highly saline water matrices needs to be elucidated. In this study, bisphenol A (BPA) was selected as the targeted micropollutant, which can be efficiently eliminated by the simulated sunlight (SSL)/PI system in the presence of high-level Cl
- (up to 846.0 mM) at pH 7.0. The presence of different background constituents of water, such as halides, nitrate, and dissolved organic matter, had no effect, or even accelerated BPA abatement. Particularly, the ubiquitous Br- or I- appreciably enhanced the BPA transformation efficiency, which may be ascribed to the generation of high-selective reactive HOBr or HOI. The in silico predictions suggested that the transformation products generated by halide-mediated SSL/PI systems via halogen substitutions showed greater persistence, bioaccumulation, and aquatic toxicity than BPA itself. These findings highlighted a widespread phenomenon during PI-based oxidative treatment of highly saline water, which needs special attention under solar light illumination., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2024
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12. Activation of periodate by biocarbon-supported multiple modified nanoscale iron for the degradation of bisphenol A in high-temperature aqueous solution.
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Zhao J, Chen J, Wang Q, Xiong R, and Ma J
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- Humans, Temperature, Iron chemistry, Water Pollutants, Chemical analysis, Benzhydryl Compounds, Periodic Acid, Phenols
- Abstract
As reported, the persistent toxic and harmful pollutant bisphenol A (BPA) from industrial emissions has been consistently found in aquatic environments inhabited by humans. Periodate (PI)-based advanced oxidation processes (AOPs) have been employed to degrade BPA, although activating PI proves more challenging compared to other oxidants. A novel nano iron metal catalyst, sulfided nanoscale iron-nickel bimetallic nanoparticle supported on biocarbon (S-(nFe
0 -Ni)/BC) was synthesized and utilized to activate PI for the removal of BPA. The morphology, structure, and composition of S-(nFe0 -Ni)/BC were characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy-energy dispersive spectrometer (SEM-EDS), and fourier-transform infrared spectrum (FTIR). The catalyst demonstrates an excellent ability to activate PI, achieving a BPA removal efficacy of 86.4%, accompanied by a 33% reduction in total organic carbon (TOC) in the {S-(nFe0 -Ni)/BC}/PI system. BPA degradation exhibited a significant change at the 5-min mark. In the first stage (0-5 min), nonlinear dynamic fitting research, combined with scavenging experiments, unveiled the competitive degradation of pollutants primarily driven by iodate radical ( IO 3 · ), singlet oxygen 1 O 2 , and hydroxyl radical ( · OH ). The competitive dynamics aligned with the ExpAssoc model. The contribution rates of different active species during the second stage (5-120 min) were calculated. The contributions of main species to BPA removal follow the order of IO 3 · > 1 O 2 > · OH throughout the entire process. The influence of various parameters, such as the dosage of S-(nFe0 -Ni)/BC, initial PI concentration, BPA concentration, pH, temperature, and the presence of coexisting anions, was also examined. Finally, a plausible reaction mechanism in the system is proposed, suggesting that the {S-(nFe0 -Ni)/BC}/PI system involves a heterogeneous synergistic reaction occurring primarily on the surface of S-(nFe0 -Ni)/BC. Therefore, this study proposes a promising approach for PI-based AOPs to degrade organic pollutants, aiming to mitigate the irreversible harm caused by such pollutants to organisms and the environment., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2024
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13. Natural pyrite-stimulative periodate activation: efficiency and mechanism study.
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Ying H and Mao Y
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- Ferric Compounds, Environmental Monitoring, Oxidation-Reduction, Ferrous Compounds, Chlortetracycline chemistry, Water Pollutants, Chemical analysis, Iron, Periodic Acid, Sulfides
- Abstract
Natural pyrite (NP) is an alternative catalyst for wastewater purification via advanced oxidation processes (AOPs). However, the activation performance and mechanism of periodate (PI) by NP have not yet been revealed. Herein, this work examines the activation performance of NP towards PI and its application in the degradation of antibiotic wastewater. Interestingly, 95.69% of chlortetracycline (CTC) was degraded by NP within 20 min via PI activation. Besides, NP shows effective degradation of various pollutants such as rhodamine B (65.81%), sulfamethoxazole (89.04%), and sodium butylxanthate (99.77%) within 20 min. The active species quenching experiment suggested that the active species ∙ OH , IO 3 ∙ ,
1 O2 and the active complex of PI bonded with NP surface participated in CTC degradation. In addition, Fe(II) on NP surface is the main active site for PI activation, while Sn 2- species accelerates the reduction of Fe(III) to Fe(II) and promotes sustained PI activation. This work provides new ideas for the application of NP in environmental pollution control., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)- Published
- 2024
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14. Highly efficient degradation of acetaminophen via nano zero-valent iron biochar with periodate system at low temperature.
- Author
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Zhuo SN, Zhang W, Ren HY, and Liu BF
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- Acetaminophen, Temperature, Wastewater, Charcoal, Iron, Water Pollutants, Chemical analysis, Periodic Acid
- Abstract
The development of more efficient advanced oxidation systems for serving various advanced treatment of wastewater is quite necessary and urgent. In this study, a nano-zero valent iron/periodate (nZVI-BC/PI) advanced oxidation system has been constructed, achieving a rapid degradation of acetaminophen (ACT, 1 mg/L) within 1 min (100 % at pH = 11) at low temperature (5℃). This system shows a great degradation in a wide range of pH (1 ∼ 11), improving the pH limitation of PI oxidation system. During the reaction process, ·OH as the main active species collaborate with
1 O2 , Fe (IV), ·O2 - and electron transfer to degrade ACT. In this system, iron ion leaching is low (0.019 mg/L), ACT was effectively degraded (74.36 %∼97.32 %) under different water, moreover, the material has an expected recyclability. The research provides a significant guidance for the advanced treatment of wastewater especially in cold regions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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15. Fluorescent periodic acid Schiff-like staining combined with standard cytologic staining of the same cytologic specimen may facilitate cytopathologic diagnosis.
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Hachisuka D, Nakano S, Takase H, Sugiyama M, Sugiura M, Fujii K, Masaki A, Murase T, and Inagaki H
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- Humans, Periodic Acid, Staining and Labeling, Azure Stains, Glycogen, Coloring Agents, Carcinoma
- Abstract
Background: Periodic acid Schiff (PAS) staining which detects glycogen and mucosubstances is frequently used as an ancillary method for an accurate cytopathologic diagnosis. Unfortunately, cytologic slides for PAS stain are not routinely prepared. Aqueous 7-amino-4-methylcoumarin (AMC) is colorless and transparent under bright field illumination but exhibits strong fluorescence under ultraviolet (UV) light and can be used as a Schiff reagent. We recently reported that combining [author: Please define (H&E) in the first occurrence if necessary.]H&E and AMC is useful for histopathologic diagnosis of various disease conditions. In this study, we investigated whether standard cytologic staining (Papanicolaou [Pap] and Giemsa) combined with AMC was useful for cytopathologic analysis., Methods: Specimens of non-neoplastic human tissues and archived cytologic specimens of various disease conditions were stained with a combination of Pap and AMC (Pap/AMC) or Giemsa and AMC (Giemsa/AMC)., Results: The addition of AMC had no significant effect on Pap or Giemsa staining, and the cytomorphology under bright field microscopy was perfectly preserved. The AMC fluorescent signals observed under UV light were intense and the staining pattern was identical to that obtained by PAS staining. Diastase digestion differentiated glycogen from other AMC-positive elements. The efficacy of using Pap/AMC and Giemsa/AMC for archived cytologic specimens was demonstrated in several diseases including cases of endometrial carcinoma, adenoid cystic carcinoma, metastatic signet-ring cell carcinoma, candidiasis, and trichomoniasis., Conclusion: Pap/AMC and Giemsa/AMC are useful in aiding cytopathologic diagnosis especially when the information gained from PAS staining is critical and cytologic specimens for PAS are not available., (© 2023 Wiley Periodicals LLC.)
- Published
- 2024
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16. Morphological and etiological analyses of C3 and non-C3 glomerulonephritis in primary membranoproliferative glomerulonephritis using periodic acid-methenamine silver stain electron microscopy: a retrospective multicentered study.
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Honma S, Sato N, Sakaguchi R, Hashiguchi A, Uesugi N, Nakamura Y, Sasano H, and Joh K
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- Humans, Methenamine, Periodic Acid, Retrospective Studies, Complement C3 analysis, Microscopy, Electron, Glomerulonephritis, Membranoproliferative, Glomerulonephritis
- Abstract
This study elucidated the etiology of C3 glomerulonephritis (C3GN) and non-C3GN with primary membranoproliferative glomerulonephritis (MPGN) using transmission electron microscopy (TEM) and periodic acid-methenamine silver stain (PAM-EM). Thirty-one primary MPGN cases were analyzed by TEM and PAM-EM to distinguish among MPGN I, MPGN II, MPGN III Burkholder subtype (MPGN IIIB), and Anders and Strife subtype (MPGN IIIA/S). Each case was also classified into C3GN or non-C3GN according to the standard C3GN definition using immunostaining. Four cases of MPGN II met C3 glomerulopathy; whereas, four cases of MPGN IIIB did not meet C3 glomerulopathy. Seven of 11 cases (64%) of MPGN I without GBM disruption and 7 of 12 cases (58%) of MPGN IIIA/S with GBM disruption met the non-C3GN criteria with significant immunoglobulins' deposition. Regardless of the C3GN or non-C3GN diagnosis, the deposits in primary MPGN I and MPGN IIIA/S exhibited ill-defined, amorphous, and foggy characteristics similar to those found in postinfectious GN but were different from immune complex (IC) deposits seen in MPGN IIIB. Not only C3GN but also non-C3GN was due to mechanisms other than IC deposition as found in postinfectious GN. Consequently, GBM disruption of MPGN IIIA/S was not due to IC deposition., (© 2023. The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.)
- Published
- 2024
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17. Recent advances on the preparation conditions, structural characteristics, physicochemical properties, functional properties and potential applications of dialdehyde starch: A review.
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Yong H and Liu J
- Subjects
- Oxidation-Reduction, Oxidants, Starch chemistry, Starch analogs & derivatives, Periodic Acid
- Abstract
Starch, a natural storage polysaccharide of plant kingdom, has many industrial applications. However, native starch has some inherent shortages, which can be overcome by structural modification. Dialdehyde starch, one kind of oxidized starch produced by periodate oxidation, has good physical properties and bioactivities with wide applications in different fields. Dialdehyde starch is typically achieved by oxidizing native starch slurry through periodate oxidation under controlled reaction conditions. Several factors including the source of starch, the type of oxidant, the molar ratio of oxidant to starch, reaction temperature, reaction time and solution pH value can influence the synthesis of dialdehyde starch. Dialdehyde starch shows different spectroscopic/chromatographic characters and physicochemical properties from native starch. Moreover, dialdehyde starch exhibits good antioxidant activity, antimicrobial activity and cross-linking property. Based on these functional properties, dialdehyde starch has shown application potentials in food packaging, thermoplastic production, enzyme immobilization, heavy metal ion adsorption, drug delivery, wood adhesion and leather tanning. In this review, the preparation conditions, structural characteristics, physicochemical properties, functional properties and potential applications of dialdehyde starch are summarized for the first time. The future research and development prospects of dialdehyde starch are also discussed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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18. A step towards tuning the jute fiber structure and properties by employing sodium periodate oxidation and coating with alginate.
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Ivanovska A, Milošević M, Lađarević J, Jankoska M, Matić T, Svirčev Z, and Kostić M
- Subjects
- Periodic Acid, Oxidation-Reduction, Hydrophobic and Hydrophilic Interactions, Alginates chemistry
- Abstract
This paper outlines a novel simple protocol for tuning the structure and properties of jute using sodium periodate (NaIO
4 ) oxidation and coating with alginate. When compared to the raw jute, fabrics oxidized with a 0.2 or 0.4 % NaIO4 solution for 30-120 min exhibited an increased aldehyde group content (0.185 vs. 0.239-0.398 mmol/g), a significantly increased negative zeta potential (from -8.57 down to -20.12 mV), a slight disruption of fiber crystallinity, 15.1-37.5 % and 27.9-49.8 % lower fabric maximum force and stiffness, respectively. Owing to the removal of hydrophobic surface barrier, decreased crystallinity index and the presence of micropores on the fabrics' surfaces, oxidized fabrics have a 22.3-29.6 % improved ability for moisture sorption compared to raw fabric. Oxidized fabrics characterized by very long wetting times and excellent antioxidant activities (> 98 %), can find applications as hydrophobic packaging materials. To further extend the utilization of jute in biocarpet engineering such as water-binding geo-prebiotic supports, oxidized fabrics were coated with alginate resulting in 7.9-24.9 % higher moisture sorption and 352-660 times lower wetting times than their oxidized counterparts. This modification protocol has never been applied to lignocellulosic fibers and sheds new light on obtaining jute fabrics with tuned structure and properties intended for various applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
19. Controlling the characteristics of raw natural rubber by partial degradation in the latex stage using a water-soluble degrading agent.
- Author
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Yusof, Nurul Hayati, Baratha Nesan, Krishna Veni, and Mohd Rasdi, Fatimah Rubaizah
- Subjects
- *
MOLECULAR weights , *RUBBER , *CHAIN scission , *NUCLEAR magnetic resonance , *LATEX , *RUBBER goods , *CARBONYL group - Abstract
Partial degradation of high ammonia natural rubber (HANR) in the latex stage was successfully carried out in the presence of periodic acid as a water-soluble degrading agent at ambient temperatures, i.e. 30 °C. The number average molecular weight (Mn), weight average of molecular weight (Mw), gel content and Mooney viscosity were decreased as the periodic acid concentrations increased. The polydispersity was slightly increased with increasing periodic acid concentrations. The relationship between Mw, gel content and Mooney viscosity was established. Mw in the range of 8.0 × 105 g/mol to 1.2 × 106 g/mol, and gel content between 30 and 45 wt% successfully achieved the Mooney viscosity of raw natural rubber ranging from 60 to 85 MU. To obtain such properties, periodic acid concentration between 17 and 19 phr was used to partially degrade the natural rubber in the latex stage. The structural characterisations determined by Fourier transform infra-red (FTIR) and nuclear magnetic resonance (NMR) spectroscopies confirmed the occurrence of chain scission to form carbonyl terminal groups as a result of partial degradation. In summary, the Mooney viscosity of raw natural rubber can be controlled by partial degradation of natural rubber in the latex stage, contributed by a controlled decrease in molecular weight and gel content. Hence, this work may be useful in altering the properties of raw natural rubber that are suitable for processing of dry rubber products. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
20. HISTOLOGICAL AND SCANNING STUDY ON THE LIVER OF NEWBORN AND ADULT WHITE SWISS MICE.
- Author
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K. A., AL-Zubaidi and Hirallah, Ahmed Ali K.
- Subjects
HISTOLOGY ,LIVER ,MICE ,EOSIN ,PERIODIC acid - Abstract
The current study was carried out to investigate the histological and ultrastructural changes that occurred in the liver of Swiss mice (Mus musculus) of different postnatal ages. To obtain this target, specimens from liver were collected from twenty mice at four different ages 1,10,30,60 postnatal days, which were set as (1st) day old (G1), 10th day (G2), 30th day (G3) and 60th day (G4) respectively. Routine histological stain, Harris Hematoxyline and Eosin (H&E) and Periodic Acid Schiff (PAS) in addition to scanning electronic microscope, were conducted to achieve current targets. The histological examination of liver revealed many events occurred with progress of ages, hematopoietic elements were decreased in number, the capsule was decrease in thickness which in adult was thinner than that in young. Also, the hepatocytes and there nucleus increased in volume with acquired hexagonal-shape. The sinusoids were decreased in diameter from high expansion to very narrow in adult stage. Central vein showed increasead in diameter. The portal area well-formed at 1st day old with contain 1-4 of bile ducts and increased in number of portal area up to adult. The glycogen was absent through the 1st day old and appeared at 10th day old up to adult with PAS stain. Adipose droplets were seen for first time in hepatocellular cytoplasmic in 30th day old with PAS. In the subsequent ages both their size and number of hematopoietic compartments were decrease. Hepatic liver lobules were initiated in 10th day old and in 30th day old the prominent boundaries were seen, Whereas, it well-formed at 60th day old. The sub-capsular hematopoietic elements appeared from 30th day old up to 60th day old. Scanning electronic microscope examination of liver revealed that the shape of hepatocyte change from polyhedral in 1st day old to hexagonal shape at the 30th day old. The hematopoietic foci seen at (G1) scattered throughout the parenchyma and become very rare, as a small foci at the (G2). The triad surrounded by the periportal plates or "limiting plates" and the central vein was have not valves. The present study concluded a many of histological changes which referred to development in the structural and functional of liver throughout the life span. [ABSTRACT FROM AUTHOR]
- Published
- 2020
21. A comprehensive investigation on modified cellulose nanocrystals and their films properties
- Author
-
Nassima El Miri, Ellinor Bævre Heggset, Sara Wallsten, Anna Svedberg, Kristin Syverud, and Magnus Norgren
- Subjects
Oxygen ,Aldehydes ,Structural Biology ,Ammonium Compounds ,Periodic Acid ,Nanoparticles ,General Medicine ,Silanes ,Cellulose ,Molecular Biology ,Biochemistry - Abstract
In this work, we aimed to tune cellulose nanocrystals (CNCs) properties by introducing different functional groups (aldehyde, carboxyl, silane, and ammonium groups) on the surface through different chemical modifications. These functional groups were obtained by combining: the periodate oxidation with TEMPO-oxidation, aminosylation or cationization. CNCs produced and their films were characterized to elucidate their performances. The results showed that the properties of obtained CNCs varied depending on the grafted functionalities on the surface. The results reveal that after each modification a colloidal stability is preserved. Interestingly, Periodate oxidation of cellulose nanocrystals results in film components that interact through intra- and intermolecular hemiacetals and lead to films with a tensile strength of 116 MPa compared to the pristine CNCs, in contrast the subsequent modifications led to lower tensile strength. Of note, remarkable thermal stability has been achieved after modifications reaching a maximum of 280 °C. The oxygen barrier properties of the films after modifications varied between 0.48 and 0.54 cm
- Published
- 2022
22. Doxycycline Suppresses Vasculogenic Mimicry in Human Pterygium Fibroblasts
- Author
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Meng-Xuan, He, Jun-Fang, Zhang, Ling, Yang, Bai, Qin, Hong-Wei, Gu, Qiu-Yang, Tang, Huai-Jin, Guan, and Hai-Hong, Shi
- Subjects
Vascular Endothelial Growth Factor A ,Neovascularization, Pathologic ,Periodic Acid ,Fibroblasts ,Pterygium ,Sensory Systems ,Cellular and Molecular Neuroscience ,Ophthalmology ,Matrix Metalloproteinase 9 ,Cell Line, Tumor ,Doxycycline ,Humans ,Matrix Metalloproteinase 2 ,Conjunctiva - Abstract
To explore the effect of doxycycline on vasculogenic mimicry (VM) formation and the potential mechanism in human pterygium fibroblasts in order to find novel targets for pterygium therapy.First, we demonstrate the existence of VM in 73 pterygium specimens by CD31 and periodic acid Schiff (PAS) dual staining. Then we used cell counting kit-8, clone formation assay and flow cytometry to prove the inhibitory effect of doxycycline on cell proliferation and apoptosis. The VM formation was evaluated through wound healing assay, cell transwell assay and three-dimensional cell culture combined with PAS staining. Finally, we used Western blot to testify the correlation of the VM and the factors in protein level preliminarily.Our results showed that VM existed in human pterygium specimens exactly. Otherwise, in human pterygium fibroblasts, doxycycline induced a dose-dependent inhibitory effect on cell proliferation and apoptosis induction. Besides, doxycycline significantly suppressed vasculogenic mimicry tube formation, cell migration and invasion. Furthermore, doxycycline impaired the expression of MMP-9, MMP-2 and VEGF which may related to pterygium VM formation.Doxycycline decelerated pterygium progression might be through inhibiting VM formation according to the downregulation of MMP-9, MMP-2 and VEGF, which may provide the basis of further studies involving doxycycline for pterygium treatment.
- Published
- 2022
23. A symptomatic intercalated duct lesion of the parotid gland: a case report with immunohistochemical and genetic analyses
- Author
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Kimihide Kusafuka, Satoshi Baba, Yoshiharu Kitani, Kazuki Hirata, Akinori Murakami, Aya Muramatsu, Kazumori Arai, and Makoto Suzuki
- Subjects
Male ,Adenoma ,Mucin-1 ,Periodic Acid ,S100 Proteins ,General Medicine ,Middle Aged ,Adenocarcinoma ,Salivary Gland Neoplasms ,Pathology and Forensic Medicine ,Ki-67 Antigen ,Amylases ,Humans ,Parotid Gland ,Keratins ,Molecular Biology ,beta Catenin - Abstract
Intercalated duct lesions (IDLs) are usually asymptomatic. We report a case of IDL, in which a palpable mass formed. The patient was a 45-year-old Japanese male, who noticed a mass in the left parotid region. The nodular lesion was well-circumscribed, but did not have a fibrous capsule or exhibit infiltrative growth. It contained a small cystic space and consisted of basaloid cells arranged in a cribriform pattern and inner ductal cells. It had some solid areas of nest-like proliferation displaying mild cellular atypia. Immunohistochemically, the luminal cells were positive for cytokeratin (CK)7 and epithelial membrane antigen, and the abluminal cells were positive for CK5/6, p63, and DOG1. S-100 protein-positive stromal cells were also seen. The lesion's cells were all positive for SOX10, and the nuclei of some basaloid cells were positive for β-catenin. The Ki-67 labeling index was 3.8%. The ductal cells contained diastase-digestion-resistant, Periodic acid Schiff-positive zymogen granules. Genetically, the lesion harbored a missense mutation in the CTNNB1 gene. We diagnosed the lesion as an IDL. As IDLs are usually small non-neoplastic lesions, symptomatic cases are rare. Based on its common immunohistochemical and genetic features, IDL may be a precursor of basal cell adenoma/adenocarcinoma, such as intercalated duct adenoma.
- Published
- 2022
24. Clinicopathological characteristics of signet‐ring cell carcinoma derived from gastric fovelar epithelium
- Author
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Zhi Shang Zhang, Wei Yi Deng, Si Lin Huang, Bin Feng Yang, Fang Heng Zhu, Bo Jiang, Su Nan Wang, and Yang Kun Wang
- Subjects
Adult ,Adenomatous Polyps ,Ki-67 Antigen ,Stomach Neoplasms ,Periodic Acid ,Gastroenterology ,Humans ,Neoplasm Recurrence, Local ,Carcinoma, Signet Ring Cell ,Epithelium - Abstract
We aimed to investigate the immunophenotype, differential diagnosis, and clinicopathological characteristics of signet-ring cell carcinoma (SRCC) derived from gastric foveolar epithelium.Clinical characteristics, endoscopic findings, histopathological features, and follow-up data of seven cases of SRCC derived from gastric foveolar epithelium with small intramucosal lesions were analyzed.Seven patients with a mean age of 38.3 years were diagnosed with SRCC derived from gastric foveolar epithelium and small intramucosal lesions, all of them were negative for CDH-1 germline mutation. The glands proliferated and expanded, and then morphologically transformed into signet-ring cells and formed clonal hyperplastic SRCC, which expanded laterally along the gastric foveolar cells to a length of 3-6 mm. Periodic acid Schiff staining was positive, while CK7 and MUC6 were negative, in all cases. Ki-67-positive cells ranged 37%-60%. During a follow-up period of 6-30 months, no patients experienced tumor recurrence or metastasis.SRCC derived from gastric foveolar epithelium is originated from the proliferative region of the bottom of the gastric pit and gland neck. It is easily missed diagnosed or misdiagnosed as it grows laterally along the gastric foveolar cells. Biological behavior, genetics, and etiology of such SRCC, as well as the clinicopathological characteristics, need to be further studied.
- Published
- 2022
25. Facile route to tri-carboxyl chitin nanocrystals from di-aldehyde chitin modified by selective periodate oxidation
- Author
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Jie, Jiang, Jiaju, Zhang, Ting, Li, Xuhui, Zhang, Yang, Wang, Bihua, Xia, Jing, Huang, Yimin, Fan, and Weifu, Dong
- Subjects
Aldehydes ,Food ,Structural Biology ,Periodic Acid ,Nanoparticles ,Chitin ,General Medicine ,Molecular Biology ,Biochemistry ,Refuse Disposal - Abstract
Chitin, a kind of polysaccharide mainly obtained from food waste, has emerged as an important biodegradable biopolymer in composite materials. The difficulty of aldehyde modification, which greatly limited the application of chitin nanocrystals, was addressed by applying a facile route of partial deacetylation followed by periodate oxidation in this study. Deacetylation occurred on the surface of both crystalline and amorphous regions, which were significantly degraded in the following periodate oxidation due to the inevitable cleavage of chitin chains, leading to an increase in the crystallinity index of obtained di-aldehyde chitin. The degree of deacetylation and periodate addition had limited improvement in the aldehyde content of di-aldehyde chitin with a maximum value of around 0.42 mmol/g. With further 2,2,6,6-tetramethylpiperidine-1-oxyl-mediated oxidation, the carboxyl content of tri-carboxyl chitin was improved to 1.58 mmol/g, which played a critical role in the dispersion efficiency and morphology of chitin nanocrystals. The obtained rod-like chitin nanocrystals with a ζ-potential value of -42 mV and an average size of 97 nm have potential application in dye-adsorption and emulsion stabilizers.
- Published
- 2022
26. Microanalysis of the stomach of southern white‐breasted hedgehog ( Erinaceus concolor ): Histological, histochemical, immunohistochemical, and scanning electron microscopic studies
- Author
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Maryam Almasi and Nader Goodarzi
- Subjects
Serotonin ,Histology ,Periodic Acid ,Stomach ,Electrons ,Glucagon ,Medical Laboratory Technology ,Dogs ,Gastric Mucosa ,Hedgehogs ,Gastrins ,Animals ,Alcian Blue ,Anatomy ,Instrumentation - Abstract
This study was designed to provide more detailed knowledge on the stomach histochemistry and immunohistochemistry in the southern white-breasted hedgehog (Erinaceus concolor). Two animals were used in the present work. Periodic acid Schiff's (PAS) and Alcian blue were used for histochemical purposes. SOX9, gastrin, serotonin, and glucagon markers were traced immunohistochemically. The mucosa was extremely folded in the fundus with numerous opening of glands. The body and pylorus mucosa were almost smooth and equipped with gastric gland openings. A simple columnar epithelium covered the stomach entirely. Cardiac glands region was mucus secreting with both positive and negative reactions to PAS. Fundic mucosa was contained cardiac glands near to the cardia, and toward the body it was divided into the light and dark zones. These zones and body contained proper gastric gland, which constituted of parietal, chief, and mucous neck cells. These glands contained PAS-positive cells on their basal portions. The pyloric glands were mucus secreting but negative for PAS. All gastric glands were Alcian blue-negative, but epithelium showed moderate reaction especially in the pylorus. SOX and gastrin were express highly in the body and fundus. The expression of serotonin and glucagon was rare. Comparatively, some similarities between the stomach of hedgehog and dog can be assumed. The present findings provide additional information concerning the histochemical characteristics and endocrine cells distribution in the stomach of the southern white-breasted hedgehog (Erinaceus concolor). Further detailed studies are required to enhance the current knowledge on histophysiology of the digestive system in this species as a pet and exotic animal. HIGHLIGHTS: The stomach was simple glandular type. The fundus was divided into light and dark zones similar to the dog. The proper gastric glands were periodic acid Schiff's positive at their basal parts.
- Published
- 2022
27. o -Semiquinone Radical and o -Benzoquinone Selectively Degrade Aniline Contaminants in the Periodate-Mediated Advanced Oxidation Process.
- Author
-
Zhou Y, Lei Y, Kong Q, Cheng F, Fan M, Deng Y, Zhao Q, Qiu J, Wang P, and Yang X
- Subjects
- Hydrogen Peroxide, Oxidation-Reduction, Benzoquinones, Aniline Compounds, Catechols, Ferric Compounds, Water Pollutants, Chemical analysis, Periodic Acid
- Abstract
Advanced oxidation processes (AOPs) often employ strong oxidizing inorganic radicals (e.g., hydroxyl and sulfate radicals) to oxidize contaminants in water treatment. However, the water matrix could scavenge the strong oxidizing radicals, significantly deteriorating the treatment efficiency. Here, we report a periodate/catechol process in which reactive quinone species (RQS) including the o -semiquinone radical ( o -SQ
•- ) and o -benzoquinone ( o -Q) were dominant to effectively degrade anilines within 60 s. The second-order reaction rate constants of o -SQ•- and o -Q with aniline were determined to be 1.0 × 108 and 4.0 × 103 M-1 s-1 , respectively, at pH 7.0, which accounted for 21% and 79% of the degradation of aniline with a periodate-to-catechol molar ratio of 1:1. The major byproducts were generated via addition or polymerization. The RQS-based process exhibited excellent anti-interference performance in the degradation of aniline-containing contaminants in real water samples in the presence of diverse inorganic ions and organics. Subsequently, we extended the RQS-based process by employing tea extract and dissolved organic matter as catechol replacements as well as metal ions [e.g., Fe(III) or Cu(II)] as periodate replacements, which also exhibited good performance in aniline degradation. This study provides a novel strategy to develop RQS-based AOPs for the highly selective degradation of aniline-containing emerging contaminants.- Published
- 2024
- Full Text
- View/download PDF
28. Thermo-activated periodate oxidation process for tetracycline degradation: Kinetics and byproducts transformation pathways.
- Author
-
Lu G, Li X, Li W, Liu Y, Wang N, Pan Z, Zhang G, Zhang Y, and Lai B
- Subjects
- Tetracycline toxicity, Tetracycline chemistry, Oxidation-Reduction, Periodic Acid, Anti-Bacterial Agents toxicity, Water Pollutants, Chemical chemistry
- Abstract
Periodate-based advanced oxidation processes have been diffusely practiced for pollutant decontamination. However, the thermo-activation of periodate process (heat/PI), an effective water pollution removal process, has been rarely discussed, and the degradation pathway of this heat/PI system requires investigation. In this work, tetracycline antibiotics were selected as the model micropollutant for the comprehensive evaluation of the heat/PI system. The heat/PI system exhibited good performance for tetracycline (TC) remediation with temperature increases. The principal reactive oxidative species in the heat/PI system was confirmed using quenching experiments and electron paramagnetic resonance experiments. Further, the potential reactive sites in the TC were identified based on the density functional theory calculation. Based on the detection results of intermediates, there was no significant difference in byproducts generated during TC degradation under various temperatures in the heat/PI system. The Toxicity Estimation Software Tool (T.E.S.T.) method was applied to calculate the individual toxicity of the byproducts. This study contributes to a comprehensive explanation of the process of the thermal activation of periodate, and in particular, it explains the source of oxidation power, the transformation of byproducts, and the toxicity of reaction systems., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
29. Efficient degradation of antibiotic wastewater by biochar derived from water hyacinth stems via periodate activation: pyridinic N and carbon structures improved the electron transfer process.
- Author
-
Duan W, Zhang M, and Zhou R
- Subjects
- Anti-Bacterial Agents, Carbon, Wastewater, Electrons, Charcoal chemistry, Tetracycline chemistry, Adsorption, Eichhornia, Water Pollutants, Chemical chemistry, Periodic Acid
- Abstract
Biochar-activated periodate (PI) is a promising technology toward antibiotic wastewater purification. However, the mechanism of pyrolysis temperature on PI activation efficiency by biochar has not yet been revealed. Herein, this work selected water hyacinth stems as raw materials to prepare biochar with different pyrolysis temperatures (400, 500, 600, and 700 °C), and applied it to degrade tetracycline (TC) wastewater through PI activation. The results show that biochar with a pyrolysis temperature of 700 °C (BC-700) possesses the best TC degradation performance (∼100% within 30 min). Besides, the degradation of TC by BC-700 is less interfered by coexisting anions and water matrix, and exhibits good reusability. Quenching experiments and open circuit voltage tests verified that IO
3 •, 1O2 , and reactive complex BC-PI* are active species involved in TC degradation. In addition, by constructing the relationship between biochar surface properties and degradation rate kobs , it was revealed that the dominant role of pyridinic N in PI adsorption and formation of reactive complexes as well as the promotion of sp2 -hybridized carbon in the electron transfer process. This work provides novel insights into the application of biochar in antibiotic wastewater treatment via PI activation.- Published
- 2024
- Full Text
- View/download PDF
30. Preordering Periodic Acid-Schiff Staining: A Quality Improvement Study.
- Author
-
Wolner ZJ, Mustin DE, and Stoff BK
- Subjects
- Humans, Periodic Acid, Coloring Agents, Staining and Labeling, Quality Improvement, Skin Neoplasms
- Abstract
Abstract: Periodic acid-Schiff (PAS) stain is a commonly used ancillary test for inflammatory and infectious dermatoses, yet infrequently changes the diagnosis. Previous studies have shown that clinical suspicion and histopathologic features are poor predictors of PAS positivity. Current appropriate use criteria from the American Society of Dermatopathology supports PAS staining when histopathologic features could be consistent with a dermatophyte infection. At the authors' institution, PAS stains are preordered on biopsies of inflammatory and infectious diagnoses to aid in a timelier sign out. Our aim was to reduce the percentage of PAS stains preordered on all dermatology specimens over a 6-month period without reducing the percentage of fungal infections identified. Review of a 12-month preintervention period found that our laboratory received 6104 biopsies for which PAS stain was preordered on 616 (10.1%). Based on a review of the preintervention period, preordering PAS on cases with clinical suspicion for cutaneous T-cell lymphoma was stopped unless there was clinical suspicion for eczematous dermatitis, vesiculobullous disorders, or fungal infection. The proposed intervention resulted in a 3.7% reduction in the number of PAS stains ordered while PAS-positivity rate remained unchanged. The described quality improvement process may be used as a model for other laboratories., Competing Interests: The authors declare no conflicts of interest., (Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
31. Chromium(VI) oxide-mediated oxidation of polyalkyl-polypyridines to polypyridine-polycarboxylic acids with periodic acid.
- Author
-
Yamazaki, Shigekazu
- Subjects
- *
CHROMIUM , *OXIDATION , *SULFURIC acid , *CHROMIUM oxide , *ACIDS , *OXIDIZING agents - Abstract
4,4′-Dicarboxy-2,2′-bipyridine was synthesized quantitatively by chromium(VI) oxide-mediated oxidation of 4,4′-dimethyl-2,2′-bipyridine or 4,4′-diethyl-2,2′-bipyridine with periodic acid as the terminal oxidant in sulfuric acid. 5,5′-Dicarboxy-2,2′-bipyridine and 6,6'-dicarboxy-2,2′-bipyridine were also synthesized by the method from the corresponding dimethyl bipyridines in excellent yields. 4,4′,4″-Tricarboxy-2,2′:6′,2″-terpyridine was obtained in 80% yield from 4,4′,4″-triethyl-2,2′:6′,2″-terpyridine, and 4,4′,4″,4′″-tetracarboxy-2,2′:6′,2″:6″,2′″-quaterpyridine was obtained in 72% yield from 4,4′,4″,4′″-tetraethyl-2,2′:6′,2″:6″,2′″-quaterpyridine by the same procedure. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
32. Oxidative phenol-arene and phenol-phenol cross-coupling using periodic acid.
- Author
-
Gao, Peng-Cheng, Chen, Huan, Grigoryants, Vladimir, and Zhang, Qiang
- Abstract
Abstract A simple, metal-free protocol for unsymmetrical biaryl coupling using H 5 IO 6 is reported. H 5 IO 6 was evaluated for a novel application in the oxidative cross-coupling of phenol-arene, phenol-phenol, and phenol-naphthol compounds. In this work, most of the couplings were completed within 30 min at ambient temperature. 30 coupling products were conveniently obtained using only 0.5 equivalent of H 5 IO 6 in HFIP. A mechanism by which the transformation occurs is proposed. Graphical abstract Image 1 [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
33. Catalyst-Free Periodate Activation by Solar Irradiation for Bacterial Disinfection: Performance and Mechanisms
- Author
-
Fuyang Liu, Zhengmao Li, Qiqi Dong, Chenyi Nie, Shuai Wang, Boaiqi Zhang, Peng Han, and Meiping Tong
- Subjects
Disinfection ,Periodic Acid ,Escherichia coli ,Sunlight ,Environmental Chemistry ,General Chemistry ,Catalysis ,Water Purification - Abstract
Periodate (PI)-based advanced oxidation process has recently attracted great attention in the water treatment processes. In this study, solar irradiation was used for PI activation to disinfect waterborne bacteria. The PI/solar irradiation system could inactivate
- Published
- 2022
34. Clinical and histopathological features of conjunctival 'Teddy bear' granuloma: A case series
- Author
-
Alejandra Lozano-Bustillo, Dolores Ríos y Valles-Valles, Ivette Hernández-Ayuso, and Abelardo A. Rodríguez-Reyes
- Subjects
Male ,Granuloma, Foreign-Body ,Periodic Acid ,COVID-19 ,General Medicine ,Conjunctivitis ,Conjunctival Diseases ,Cicatrix ,Ophthalmology ,Adrenal Cortex Hormones ,Child, Preschool ,Formaldehyde ,Communicable Disease Control ,Eosine Yellowish-(YS) ,Humans ,Female ,Child ,Hematoxylin ,Pandemics - Abstract
Purpose To describe the clinical features, histopathological findings, and prognosis of conjunctival Teddy bear granuloma (TBG) diagnosed in a Mexican ophthalmologic referral center in a period of 64 years. Methods We reviewed clinical and histopathological material from all patients with documented conjunctival TBG. Patient′s age, gender, location, clinical signs and symptoms, duration, treatment and, specimen size, were evaluated. Formalin-fixed paraffin embedded 5 microns hematoxylin-eosin stained slides and periodic acid-Schiff special stain as well as examination under polarized light microscopy were performed in all cases. Results A total of 5 conjunctival TBG were collected. The ages of the patients ranged between 2 and 11 with a mean of 6 years. Female to male ratio was 4:1. The right inferior fornix was involved in 4 cases (80%) with clinical diagnosis of conjunctival mass of unknown etiology. Treatment consisted of surgical removal by slit-lamp biomicroscopy (3 cases) and in the operating room (2 cases). No complications were informed in a short follow-up period. Histopathologically, numerous cross-sections of synthetic and non-synthetic fibers surrounded by multinucleated foreign body giant cells, epithelioid histiocytes, lymphocytes, plasma cells and sparse neutrophils were seen. Polarization light microscopy demonstrated numerous birefringent colorful foreign fibers. The follow-up period of the patients ranged between 1 and 32 weeks with a mean of 10 weeks. Conclusions Most patients with TBG are children with vague, non-specific symptoms troubling precise clinical diagnosis. Treatment of choice involves surgical excision of the foreign body granuloma followed by topical corticosteroids. All surgical specimens obtained must be sent for histopathological examination. Special awareness is recommended during COVID-19 pandemic lockdown to prevent potential exposure to fibers that may cause conjunctival TBG.
- Published
- 2022
35. Effects of PM2.5 on mucus secretion and tissue remodeling in a rabbit model of chronic rhinosinusitis.
- Author
-
Zhao, Renwu, Guo, Zhiqiang, Dong, Weiyang, Deng, Congrui, Han, Zhijin, Liu, Jian, Wang, Hanwei, Zhuang, Guoshun, and Zhang, Ruxin
- Subjects
- *
PATHOLOGICAL physiology , *SCANNING electron microscopy , *PERIODIC acid , *SINUSITIS , *POLYPS - Abstract
Background: According to epidemiologic studies, fine particulate matter (particulate matter ≤2.5 μm, PM2.5) is closely associated with increases in the incidence and severity of chronic rhinosinusitis (CRS). However, the role of PM2.5 in the pathophysiology of CRS remains largely unknown. In this study, we investigated the effects of PM2.5 on nasal cilia, tissue remodeling, and mucus hypersecretion in a rabbit model of CRS. Methods: CRS rabbits were exposed to ambient PM2.5 via a PM2.5 inhalation exposure system. The degree of ciliary damage was evaluated using scanning electron microscopy (SEM). Histopathologic changes were determined using periodic acid‐Schiff (PAS) staining of goblet cells and Masson trichrome (MT) staining for collagen in the nasal mucosa. MUC5AC expression was assessed by immunohistochemistry. Results: PM2.5 exposure substantially aggravated ciliary disruption in rabbits with CRS. PM2.5 also significantly increased goblet cell hyperplasia and collagen deposition, as reflected by PAS and MT staining. The nasal mucosa of CRS rabbits displayed markedly elevated MUC5AC expression after PM2.5 exposure. Conclusion: Increases in ciliary disruption, nasal remodeling, and mucus hypersecretion may be the pathophysiologic mechanisms underlying the PM2.5‐induced aggravation of CRS. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
36. Electrochemical oxidation of the iodate ion
- Author
-
Mc Ardle, Siobhan
- Subjects
541 ,Dialdehyde starch ,Periodic acid ,Periodate - Published
- 1994
37. A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases
- Author
-
Satoshi Nojima, Shoichi Ishida, Kei Terayama, Katsuhiko Matsumoto, Takahiro Matsui, Shinichiro Tahara, Kenji Ohshima, Hiroki Kiyokawa, Kansuke Kido, Koto Ukon, Shota Y. Yoshida, Tomoki T. Mitani, Yuichiro Doki, Tsunekazu Mizushima, Yasushi Okuno, Etsuo A. Susaki, Hiroki R. Ueda, and Eiichi Morii
- Subjects
Hydrazines ,Imaging, Three-Dimensional ,Crohn Disease ,Staining and Labeling ,Hepatology ,Polysaccharides ,Periodic Acid ,Gastroenterology ,Humans ,Colitis, Ulcerative ,Inflammatory Bowel Diseases - Abstract
Tissue-clearing and three-dimensional (3D) imaging techniques aid clinical histopathological evaluation; however, further methodological developments are required before use in clinical practice.We sought to develop a novel fluorescence staining method based on the classical periodic acid-Schiff stain. We further attempted to develop a 3D imaging system based on this staining method and evaluated whether the system can be used for quantitative 3D pathological evaluation and deep learning-based automatic diagnosis of inflammatory bowel diseases.We successfully developed a novel periodic acid-FAM hydrazide (PAFhy) staining method for 3D imaging when combined with a tissue-clearing technique (PAFhy-3D). This strategy enabled clear and detailed imaging of the 3D architectures of crypts in human colorectal mucosa. PAFhy-3D imaging also revealed abnormal architectural changes in crypts in ulcerative colitis tissues and identified the distributions of neutrophils in cryptitis and crypt abscesses. PAFhy-3D revealed novel pathological findings including spiral staircase-like crypts specific to inflammatory bowel diseases. Quantitative analysis of crypts based on 3D morphologic changes enabled differential diagnosis of ulcerative colitis, Crohn's disease, and non-inflammatory bowel disease; such discrimination could not be achieved by pathologists. Furthermore, a deep learning-based system using PAFhy-3D images was used to distinguish these diseases The accuracies were excellent (macro-average area under the curve = 0.94; F1 scores = 0.875 for ulcerative colitis, 0.717 for Crohn's disease, and 0.819 for non-inflammatory bowel disease).PAFhy staining and PAFhy-3D imaging are promising approaches for next-generation experimental and clinical histopathology.
- Published
- 2022
38. Iodometry in Alkaline Medium, Iodatometry, Periodimetry, and Bromometry
- Author
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Burgot, Jean-Louis and Burgot, Jean-Louis
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- 2012
- Full Text
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39. Clear cell urothelial carcinoma of bladder: Case report of a rare and aggressive variant with review of literature.
- Author
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Sahetia KM, Dave VN, Pal M, and Menon S
- Subjects
- Male, Humans, Middle Aged, Urinary Bladder pathology, Periodic Acid, Biomarkers, Tumor genetics, Carcinoma, Transitional Cell chemistry, Carcinoma, Transitional Cell diagnosis, Urinary Bladder Neoplasms diagnosis, Urinary Bladder Neoplasms pathology, Carcinoma, Renal Cell pathology, Kidney Neoplasms pathology
- Abstract
Clear cell urothelial carcinoma is a rare variant of urothelial carcinoma. It's recognition and accurate diagnosis are essential in deciding appropriate treatment protocols considering the prognosis of this variant. A 57-year-old male presented with a history of hematuria and lower urinary tract symptoms for 6 months. Microscopically, the tumor was arranged in sheets and had a nested pattern. The tumor was composed of round to polygonal cells with abundant clear cytoplasm (>90% clear cell differentiation), resembling a conventional clear renal cell carcinoma. On special stain, the tumor was positive for periodic acid-Schiff (PAS) and negative for periodic acid-Schiff with diastase (PAS-D) and mucicarmine stain. The urothelial origin of clear cells was confirmed by positivity for GATA Binding protein 3(GATA3) and High Molecular Weight Cytokeratin (HMWCK) immunohistochemistry and negativity for NK3 homeobox 1(NKX3.1), Prostate specific antigen (PSA) and Paired box gene 8 (PAX8) immunohistochemistry., (Copyright © 2023 Copyright: © 2023 Indian Journal of Cancer.)
- Published
- 2023
- Full Text
- View/download PDF
40. Detection of bacteria in dental samples using the Periodic acid-Schiff (PAS) histological stain.
- Author
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Giardino L, Generali L, Del Fabbro M, Tartaglia GM, Bidossi A, and Savadori P
- Subjects
- Humans, Periodic Acid, Staining and Labeling, Microscopy, Coloring Agents, Bacteria
- Abstract
The bacterial cell wall mainly consists of glycoproteins and polysaccharides, which could be detected in dental tissue with specific stain protocols. The present study aimed to investigate bacteria stainability in dental histological samples of human teeth by a histochemical method. Eight extracted teeth, because severely decayed, were decalcified, dehydrated, paraffin-embedded, and serially sectioned at 4 µm thickness each. The serial sections were then stained with Periodic acid-Schiff (PAS). Furthermore, SEM analysis was performed on the same slide of one previously histologically investigated tooth to acquire more details on the structures stained by the PAS method obtained from the histological procedures. Afterward, some American Type Culture Collection (ATCC) strains, smeared on glass slides, were stained following the staining method used in histological samples. Stained rod and cocci forms by PAS stain, observed under light microscopy, were predominantly detected inside dentinal tubules and root canal space of histologically examined specimens, suggesting their bacterial origin. Additional SEM analysis on the identical histological stained slide showed the precise nature of these forms (bacteria) and supplemental information regarding their vitality status. In addition, ATCC smeared strain samples showed variable PAS stainability of microorganisms investigated. Due to its properties, the PAS histochemical stain could be a valid and helpful aid for non- or weakly stainable microorganisms in infected tissues to be associated with other methods of investigation., Competing Interests: Declaration of Competing Interest The author declares that there are no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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- View/download PDF
41. Malakoplakia with aberrant ALK expression by immunohistochemistry: a case report.
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Zhang XY, Li J, Chen SL, Li Y, Wang H, and He JH
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- Female, Humans, Aged, Anaplastic Lymphoma Kinase, Immunohistochemistry, In Situ Hybridization, Fluorescence, Periodic Acid, Malacoplakia diagnosis
- Abstract
Background: Malakoplakia is a rare inflammatory disease of the urogenital tract. There have been no reports of malakoplakia expressing anaplastic lymphoma kinase (ALK) to date. Here, we present one case of malakoplakia with aberrant ALK expression by immunohistochemistry and discuss the clinical significance., Case Presentation: A 65-year-old Chinese woman with a history of diabetes presented with solid masses in the liver and kidney and elevated lesions on the mucosal surface of the colon. Right nephrectomy and partial liver resection were performed. Microscopically, sheets of histiocytes with poor intercellular adhesion were seen, with Michaelis-Gutmann bodies present in both the intracellular and extracellular interstitium. CD10-, CD68-, and CD163-positive cells were present, with Michaelis-Gutmann bodies confirmed by staining with Alcian blue, periodic acid-Schiff (PAS), periodic acid-Schiff with diastase, Von Kossa, and Prussian blue. Aberrant ALK1 and ALK (D5F3) expression was observed in the cytoplasm and nucleus of cells. However, ALK gene mutation was not detected by fluorescence in situ hybridization or whole exome next-generation sequencing. NGS revealed nine individual somatic gene mutations: GOT1L1, GLIS2, SPOUT1, TMEM97, MUC3A, NSD2, SFXN5, ADAD1 and RAD50. The significance of the somatic gene mutations detected in this study is not clear, and the relationship between them and malakoplakia cannot be clarified by existing scientific studies. The pathological diagnosis was malakoplakia with aberrant ALK expression by immunohistochemistry. The antibiotics imipenem and vancomycin were started based on the results of drug sensitivity analysis and the patient was subsequently discharged. She experienced no discomfort during 30 months of follow-up., Conclusion: This is the first reported case of malakoplakia with aberrant ALK expression, it should be differentiated from ALK-positive histiocytosis to avoid misdiagnosis., (© 2023. BioMed Central Ltd., part of Springer Nature.)
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- 2023
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- View/download PDF
42. Improved Dewaterability of Waste Activated Sludge by Fe(II)-Activated Potassium Periodate Oxidation
- Author
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Hong Xiao, Qing Liu, Yingjun Wang, Ying Zhu, Dexin Fang, Ganxue Wu, Zhenxing Zeng, and Hong Peng
- Subjects
sludge dewaterability ,ferrous ion ,potassium periodate ,extracellular polymeric substances ,Sewage ,Potassium Compounds ,Health, Toxicology and Mutagenesis ,Periodic Acid ,Public Health, Environmental and Occupational Health ,Ferrous Compounds - Abstract
Fe(II)-activated potassium periodate (KIO4) oxidation was used to improve the dewaterability of waste-activated sludge for the first time. Compared with those of raw sludge, the capillary suction time (CST), specific resistance filtration (SRF), and water content of filter cake (WC) of sludge treated using the Fe(II)/KIO4 process under the optimal conditions (i.e., the initial pH = 6.8, KIO4 dose = 1.4 mmol/g volatile suspended solids, Fe(II)/KIO4 molar ratio = 1.2) decreased by 64.34%, 84.13%, and 6.69%, respectively. For conditioned sludge flocs, the Zeta potential and particle size were increased, and hydrophilic proteins in extracellular polymeric substances (EPS) were partly degraded, accompanied by the transformation of tightly bound EPS into soluble EPS and the conversion of dense sludge flocs into loose and porous ones. During Fe(II)/KIO4 oxidation, Fe(IV) and the accompanying •OH were determined as the predominant reactive species and the underlying mechanism of sludge EPS degradation was proposed. This work provides a prospective method for conditioning the sludge dewaterability.
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- 2022
43. [Mechanism of 'Ephedrae Herba-Descurainiae Semen Lepidii Semen' combination in treatment of bronchial asthma based on network pharmacology and experimental verification]
- Author
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Bei-Bei, Zhang, Meng-Nan, Zeng, Qin-Qin, Zhang, Ru, Wang, Ju-Fang, Jia, Peng-Li, Guo, Meng, Liu, Wei-Sheng, Feng, and Xiao-Ke, Zheng
- Subjects
Interleukin-6 ,Ovalbumin ,Ephedra ,Periodic Acid ,Network Pharmacology ,Asthma ,Dexamethasone ,Interleukin-10 ,Rats ,ErbB Receptors ,Molecular Docking Simulation ,Drug Combinations ,Phosphatidylinositol 3-Kinases ,Animals ,Eosine Yellowish-(YS) ,Cyclin D1 ,Mitogen-Activated Protein Kinase 8 ,Quercetin ,RNA, Messenger ,Hematoxylin ,Proto-Oncogene Proteins c-akt ,Drugs, Chinese Herbal - Abstract
This study aims to investigate mechanism ofquot;Ephedrae Herba-Descurainiae Semen Lepidii Semenquot; combination(MT) in the treatment of bronchial asthma based on network pharmacology and in vivo experiment, which is expected to lay a theoretical basis for clinical application of the combination. First, the potential targets of MT in the treatment of bronchial asthma were predicted based on network pharmacology, and thequot;Chinese medicine-active component-target-pathway-diseasequot; network was constructed, followed by Gene Oncology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment of the potential targets. Molecular docking was used to determine the binding activity of key candidate active components to hub genes. Ovalbumin(OVA, intraperitoneal injection for sensitization and nebulization for excitation) was used to induce bronchial asthma in rats. Rats were classified into control group(CON), model group(M), dexamethasone group(DEX, 0.075 mg·kg~(-1)), and MT(1∶1.5) group. Hematoxylin and eosin(HE), Masson, and periodic acid-Schiff(PAS) staining were performed to observe the effect of MT on pathological changes of lungs and trachea and goblet cell proliferation in asthma rats. The levels of transforming growth factor(TGF)-β1, interleukin(IL)6, and IL10 in rat serum were detected by enzyme-linked immunosorbent assay(ELISA), and the mRNA and protein levels of mitogen-activated protein kinase 8(MAPK8), cyclin D1(CCND1), IL6, epidermal growth factor receptor(EGFR), phosphatidylinositol 3-kinase(PI3 K), and protein kinase B(Akt) by qRT-PCR and Western blot. Network pharmacology predicted that MAPK8, CCND1, IL6, and EGFR were the potential targets of MT in the treatment of asthma, which may be related to PI3 K/Akt signaling pathway. Quercetin and β-sitosterol in MT acted on a lot of targets related to asthma, and molecular docking results showed that quercetin and β-sitosterol had strong binding activity to MAPK, PI3 K, and Akt. In vivo experiment showed that MT could effectively alleviate the symptoms of OVA-induced asthma rats, improve the pathological changes of lung tissue, reduce the production of goblet cells, inhibit the inflammatory response of asthma rats, suppress the expression of MAPK8, CCND1, IL6, and EGFR, and regulate the PI3 K/Akt signaling pathway. Therefore, MT may relieve the symptoms and inhibit inflammation of asthma rats by regulating the PI3 K/Akt signaling pathway, and quercetin and β-sitosterol are the candidate active components.
- Published
- 2022
44. Pathological Comparison of Rat Pulmonary Models Induced by Silica Nanoparticles and Indium-Tin Oxide Nanoparticles
- Author
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Yi Guan, Nan Liu, Yan Yu, Qiang Zhou, Meiyu Chang, Yongheng Wang, and Sanqiao Yao
- Subjects
Male ,Biophysics ,Pharmaceutical Science ,Metal Nanoparticles ,Bioengineering ,Indium ,Collagen Type I ,Biomaterials ,Rats, Sprague-Dawley ,International Journal of Nanomedicine ,Drug Discovery ,Animals ,Hematoxylin ,Lung ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Organic Chemistry ,Periodic Acid ,Tin Compounds ,General Medicine ,Pneumonia ,Silicon Dioxide ,Fibrosis ,Rats ,Eosine Yellowish-(YS) ,Nanoparticles ,Bronchoalveolar Lavage Fluid - Abstract
Yi Guan,1 Nan Liu,1 Yan Yu,1 Qiang Zhou,1,2 Meiyu Chang,1,2 Yongheng Wang,1 Sanqiao Yao1,2 1School of Public Health, North China University of Science and Technology, Tangshan, Peopleâs Republic of China; 2School of Public Health, Xinxiang Medical University, Xinxiang, Peopleâs Republic of ChinaCorrespondence: Sanqiao Yao, Email sanqiaoyao@xxmu.edu.cnPurpose: The objective of this study was to evaluate and compare the histopathological implications of silica nanoparticles (Nano-SiO2) and indium-tin oxide nanoparticles (Nano-ITO), in vivo.Methods: Male Sprague-Dawley rats were exposed to Nano-SiO2 (50 mg/kg) and Nano-ITO (6 mg/kg) by a single intratracheal instillation, respectively. Broncho-alveolar lavage fluid (BALF) and lung tissue were obtained at 7, 14, 28, and 56 days post exposure for analysis of BALF inflammatory factors, total protein, and for lung tissue pathology. Histopathological and ultrastructural change in lungs were investigated by hematoxylin and eosin, Massonâs trichrome, sirius red staining, periodic acid Schiff stain, and transmission electron microscopy. The expression of SP-A, collagen type I and III in lung tissue was determined by immunohistochemistry and ELISA.Results: The rats in both models exhibited obvious collagen fibrosis and the severity of the lung injury increased with time after exposure to respective dosage increased. Several parameters of pulmonary inflammation and fibrosis significantly increased in both groups, which was reflected by increased LDH activity, total proteins, TNF-α, and IL-6 levels in BALF, and confirmed by histopathological examination. The results also showed that the two models exhibited different features. Exposure to Nano-ITO caused persistent chronic lung inflammation, illustrated by the infiltration of a large amount of enlarged and foamy macrophages and neutrophils into the lung parenchyma. In Nano-SiO2 exposed rat lung tissue, granulomatous inflammation was most prominent followed by progressive and massive fibrotic nodules. Compared with the Nano-SiO2 rats, Nano-ITO exposed rats exhibited significantly severe pulmonary alveolar proteinosis (PAP) pathological changes, lower fibrosis, and higher levels of inflammatory biomarkers. However, Nano-SiO2 exposed rats had greater fibrosis pathological changes and more severe granulomas than Nano-ITO exposed rats.Conclusion: This study suggests that the Nano-SiO2-induced model has greater value in research into granulomas and fibrosis, while the Nano-ITO-induced model has greater repeatability in area of PAP.Keywords: silica nanoparticles, indium-tin oxide nanoparticles, silicosis, indium lung disease, pulmonary alveolar proteinosis
- Published
- 2022
45. Histological Procedures Carried out During Coconut Somatic Embryogenesis
- Author
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Luis Sáenz, Carbonell, Carlos Oropeza, Salín, Ben Hur Chuc, Armendariz, and Alfonso Azpeitia, Morales
- Subjects
Cocos ,Staining and Labeling ,Periodic Acid ,Embryonic Development - Abstract
Histological methods allow to characterize the developmental changes that occur in plant tissues during in vitro culturing of explants. Different procedures to assess these changes have been reported in the literature. Here we describe a simple histological procedure to evaluate the development of explants using the fixation with paraformaldehyde, embedding with methacrylate resin and staining with periodic acid Schiff's reagent and naphthol blue black. This method allows to obtain high contrast in the stained cells, for a better characterization of cellular events that occur during coconut somatic embryogenesis.
- Published
- 2022
46. [The value of Alcian blue periodic acid Schiff staining and Ki-67 expression in diagnosing gastric reactive epithelial hyperplasia and dysplasia]
- Author
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Z Y, Shi, W H, Hou, Y, Wang, Z Q, Tian, Q, Cao, X M, Guo, J, Lu, X, Li, H, Chen, and M L, Jin
- Subjects
Hyperplasia ,Ki-67 Antigen ,Staining and Labeling ,Stomach Neoplasms ,Periodic Acid ,Humans ,Alcian Blue - Published
- 2022
47. Controlled degradation of polyisoprene and polybutadiene: A comparative study of two methods.
- Author
-
Berto, Pierre, Grelier, Stéphane, and Peruch, Frédéric
- Subjects
- *
POLYISOPRENE , *POLYBUTADIENE , *CHEMICAL decomposition , *ALDEHYDES , *PERIODIC acid - Abstract
Low molar mass carbonyl telechelic cis,1–4 polyisoprene (CTPI) and aldehyde telechelic cis, 1–4 polybutadiene (ATPB) were easily prepared by the controlled degradation of high molar mass 1,4- cis polyisoprene (PI) and 1,4- cis polybutadiene (PB) in a molar mass range of 5000–80 000 g mol −1 . Two methods are compared: the direct one using only the periodic acid (H 5 IO 6 ) to randomly cleave the chain and a second one where the chain are first epoxidized before being cleaved by the periodic acid. In both cases, a control of the final chain length was observed with nevertheless a better control of the final chain length, dispersity and chain-ends using the 2-steps procedure. Importance of the washing step in order to avoid side reactions on the carbonyl chain-ends is also discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
48. Paper miniaturization via periodate oxidation of cellulose.
- Author
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Strong, E. Brandon, Kirschbaum, C. Ward, Martinez, Andres W., and Martinez, Nathaniel W.
- Subjects
CELLULOSE ,PAPER ,MICROFABRICATION ,SODIUM ,CHROMATOGRAPHIC analysis - Abstract
Abstract: Cellulose-based paper is a versatile material with a diverse array of applications. While paper is not commonly thought of as a material that shrinks, here we present a method for miniaturizing paper via periodate oxidation. Chromatography paper was exposed to varying concentrations of periodate (0.1-0.5 M) over a 96-h period. Following optimization of miniaturization parameters, fourteen different types of paper were miniaturized and reductions in surface area ranging from 60 to 80% were observed. All cellulose paper types, but not cellulose-derivatives, displayed successful miniaturization. Results were highly tunable dependent upon periodate concentration and reaction time. Potential applications of the technique are discussed, including its use as a microfabrication method.Graphical Abstract:
[ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
49. Evaluation of a new fluorescent reagent, fluorescent brightener 85, for the diagnosis of suspected onychomycosis compared with potassium hydroxide.
- Author
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Yue, Xueping, Wang, Aiping, Wang, Hongwei, Li, Qing, and Yue, Xiuling
- Subjects
- *
MYCOSES , *POTASSIUM hydroxide , *CANDIDA , *NAIL diseases , *PERIODIC acid - Abstract
Summary: Onychomycosis is a common fungal infection in dermatology clinics. The commonly used diagnostic method, potassium hydroxide (KOH) direct microscopy, does not have a high‐positive detection rate. Therefore, a new diagnostic method is needed to confirm onychomycosis. The objective of this research was to use fluorescent brightener 85 (FB 85), a new fluorescent reagent for fungi detection, and to evaluate this new method based on the diagnostic positive rate, accuracy and efficiency in suspected onychomycosis compared with the KOH method. Subungual debris was collected from clinically suspected onychomycosis cases and divided into two parts. The parts were examined by FB 85 and 10% KOH solution, respectively. In total, 108 patients with suspected onychomycosis were recruited. The positive rates of FB 85 and KOH were 88.9% and 55.6%, respectively. The patients showed significant statistical differences (
P < .001). Compared with the KOH method, the FB 85 fluorescent method was an accurate and efficient method with a higher positive rate. This method could increase the diagnostic accuracy and efficiency of onychomycosis detection. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
50. Preparation of Liquid Epoxidized Natural Rubber by Oxidative Degradations Using Periodic Acid, Potassium Permanganate and UV-Irradiation.
- Author
-
Rooshenass, Pejvak, Yahya, Rosiyah, and Gan, Seng Neon
- Subjects
EPOXY compounds ,RUBBER ,PERIODIC acid ,POTASSIUM permanganate ,ULTRAVIOLET radiation ,GEL permeation chromatography - Abstract
Epoxidized natural rubber (ENR) needs to be degraded into shorter chain lengths, to form liquid epoxidized natural rubber (LENR), for applications such as coating and adhesives. Since ENR contains both C=C and epoxide groups as reactive sites for degradation reactions, thus, LENR could be prepared by different methods through cleavages of C=C or epoxide groups, or a combination of both sites. Different mechanisms would produce different terminals on the LENR. This paper reports the oxidative degradation by (a) periodic acid, (b) potassium permanganate and (c) ultra violet (UV) irradiation. The degraded rubbers were characterized by gel permeation chromatography (GPC), nuclear magnetic resonance (NMR) and Fourier transform infra-red spectroscopy (FTIR). Ester and ketone terminals were formed in all the three methods, but lactone and hydrofuranic structures were observed only in degradation by UV irradiation. NMR spectrum reveals that cyclization of ENR has occurred during degradation by periodic acid. At lower periodic acid concentration, degradation takes place only via C=C cleavage, but at higher concentration, the attack to the epoxide groups becomes more prominent. Potassium permanganate has attacked both the double bonds and epoxide groups. On the other hands, epoxide group was not affected during degradation by UV irradiation, which cleaved only the C=C bonds. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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