1. Glycoprotein 5-Derived Peptides Induce a Protective T-Cell Response in Swine against the Porcine Reproductive and Respiratory Syndrome Virus.
- Author
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Calderon-Rico, Fernando, Bravo-Patiño, Alejandro, Mendieta, Irasema, Perez-Duran, Francisco, Zamora-Aviles, Alicia Gabriela, Franco-Correa, Luis Enrique, Ortega-Flores, Roberto, Hernandez-Morales, Ilane, and Nuñez-Anita, Rosa Elvira
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PORCINE reproductive & respiratory syndrome , *CYTOTOXIC T cells , *CARRIER proteins , *PEPTIDES , *T cells , *VIRUS cloning , *CELLULAR immunity - Abstract
We analyzed the T-cell responses induced by lineal epitopes of glycoprotein 5 (GP5) from PRRSV to explore the role of this protein in the immunological protection mediated by T-cells. The GP5 peptides were conjugated with a carrier protein for primary immunization and booster doses. Twenty-one-day-old pigs were allocated into four groups (seven pigs per group): control (PBS), vehicle (carrier), PTC1, and PTC2. Cytokine levels were measured at 2 days post-immunization (DPI) from serum samples. Cytotoxic T-lymphocytes (CTLs, CD8+) from peripheral blood were quantified via flow cytometry at 42 DPI. The cytotoxicity was evaluated by co-culturing primed lymphocytes with PRRSV derived from an infectious clone. The PTC2 peptide increased the serum concentrations of pro-inflammatory cytokines (i.e., TNF-α, IL-1β, IL-8) and cytokines that activate the adaptive cellular immunity associated with T-lymphocytes (i.e., IL-4, IL-6, IL-10, and IL-12). The concentration of CTLs (CD8+) was significantly higher in groups immunized with the peptides, which suggests a proliferative response in this cell population. Primed CTLs from immunized pigs showed cytolytic activity in PRRSV-infected cells in vitro. PTC1 and PTC2 peptides induced a protective T-cell-mediated response in pigs immunized against PRRSV, due to the presence of T epitopes in their sequences. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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