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Your search keyword '"Perera, Harini A."' showing total 11 results
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11 results on '"Perera, Harini A."'

2. Imaging, Identification and Inhibition of Microorganisms Using AIEgens

Author

Perera, Harini A., Yan, Mingdi, Olivucci, Massimo, Editor-in-Chief, Wong, Wai-Yeung, Editor-in-Chief, Bayley, Hagan, Series Editor, Hughes, Greg, Series Editor, Hunter, Christopher A., Series Editor, Hwang, Seong-Ju, Series Editor, Ishihara, Kazuaki, Series Editor, Kirchner, Barbara, Series Editor, Krische, Michael J., Series Editor, Larsen, Delmar, Series Editor, Lehn, Jean-Marie, Series Editor, Luque, Rafael, Series Editor, Siegel, Jay S., Series Editor, Thiem, Joachim, Series Editor, Venturi, Margherita, Series Editor, Wong, Chi-Huey, Series Editor, Wong, Henry N.C., Series Editor, Yam, Vivian Wing-Wah, Series Editor, Yan, Chunhua, Series Editor, You, Shu-Li, Series Editor, Tang, Youhong, editor, and Tang, Ben Zhong, editor

Published
2022
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6. Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe.

Author

Qing Xia, Perera, Harini A., Bolarinho, Rylie, Piskulich, Zeke A., Zhongyue Guo, Jiaze Yin, Hongjian He, Mingsheng Li, Xiaowei Ge, Qiang Cui, Ramström, Olof, Mingdi Yan, and Ji-Xin Cheng

Subjects
*CARBOHYDRATES, *CLICK chemistry, *INTRACELLULAR tracking, *TREHALOSE, *FLUORESCENT probes, *GLYCANS
Abstract

Real-time tracking of intracellular carbohydrates remains challenging. While click chemistry allows bio-orthogonal tagging with fluorescent probes, the reaction permanently alters the target molecule and only allows a single snapshot. Here, we demonstrate click-free mid-infrared photothermal (MIP) imaging of azide-tagged carbohydrates in live cells. Leveraging the micromolar detection sensitivity for 6-azido-trehalose (TreAz) and the 300-nm spatial resolution of MIP imaging, the trehalose recycling pathway in single mycobacteria, from cytoplasmic uptake to membrane localization, is directly visualized. A peak shift of azide in MIP spectrum further uncovers interactions between TreAz and intracellular protein. MIP mapping of unreacted azide after click reaction reveals click chemistry heterogeneity within a bacterium. Broader applications of azido photothermal probes to visualize the initial steps of the Leloir pathway in yeasts and the newly synthesized glycans in mammalian cells are demonstrated. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe.

Author

Xia Q, Perera HA, Bolarinho R, Piskulich ZA, Guo Z, Yin J, He H, Li M, Ge X, Cui Q, Ramström O, Yan M, and Cheng JX

Subjects
Humans, Trehalose metabolism, Trehalose chemistry, Carbohydrates chemistry, Fluorescent Dyes chemistry, Biological Transport, Azides chemistry, Click Chemistry methods
Abstract

Real-time tracking of intracellular carbohydrates remains challenging. While click chemistry allows bio-orthogonal tagging with fluorescent probes, the reaction permanently alters the target molecule and only allows a single snapshot. Here, we demonstrate click-free mid-infrared photothermal (MIP) imaging of azide-tagged carbohydrates in live cells. Leveraging the micromolar detection sensitivity for 6-azido-trehalose (TreAz) and the 300-nm spatial resolution of MIP imaging, the trehalose recycling pathway in single mycobacteria, from cytoplasmic uptake to membrane localization, is directly visualized. A peak shift of azide in MIP spectrum further uncovers interactions between TreAz and intracellular protein. MIP mapping of unreacted azide after click reaction reveals click chemistry heterogeneity within a bacterium. Broader applications of azido photothermal probes to visualize the initial steps of the Leloir pathway in yeasts and the newly synthesized glycans in mammalian cells are demonstrated.

Published
2024
Full Text
View/download PDF

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