367 results on '"Pereira JC"'
Search Results
2. HEMOVIGILÂNCIA – ANÁLISE DE REAÇÕES TRANSFUSIONAIS NOTIFICADAS NUMA AGÊNCIA TRANSFUSIONAL DO GRUPO GSH
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Barbosa, ML, primary, Alves, BE, additional, Neta, AMB, additional, Freitas, AFA, additional, Alves, SNT, additional, Cerqueira, MAF, additional, Pereira, JC, additional, Machado, LMS, additional, and Silva, AJ, additional
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- 2023
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3. Partial pulpotomy and tooth reconstruction of a crown-fractured permanent incisor: a case report.
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Svizero NR, Bresciani E, Francischone CE, Franco EB, and Pereira JC
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Pulp exposure due to traumatic injuries in the anterior permanent teeth of adolescents is a common occurrence. A vital permanent maxillary incisor with complex crown fracture and pulp exposure was treated by partial pulpotomy and assessed clinically through pulpal sensitivity tests and radiographically for periapical healing. Partial pulpotomy consisted of pulp tissue removal to a depth of only 1 to 2 mm, then capping the pulpal wound with calcium hydroxide, lining with resin-modified glass-ionomer cement, and restoration with resin composite. At each recall (7, 15, 21, 50, 90, 150, and 250 days), no spontaneous pain was observed; the pulp showed signs of vitality and absence of periapical radiolucency after 90 days. For long-term success, partial pulpotomy is recommended as an option for cases of traumatic pulp exposure in permanent incisors with crown fractures. [ABSTRACT FROM AUTHOR]
- Published
- 2003
4. Treatment of Dentin Hypersensitivity Using Nano-Hydroxyapatite Pastes: A Randomized Three-Month Clinical Trial
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Wang, L, primary, Magalhães, AC, primary, Francisconi-dos-Rios, LF, primary, Calabria, MP, primary, Araújo, DFG, primary, Buzalaf, MAR, primary, Lauris, JRP, primary, and Pereira, JC, primary
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- 2016
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5. Biological Restorations as an Alternative to Reconstructing Posterior Teeth: A Case Report
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Albuquerque, NLG, primary, Mendonça, JS, primary, Fonteles, CSR, primary, Pereira, JC, primary, and Santiago, SL, primary
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- 2015
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6. Distribution of 137Cs in Soils Due to the Goiania Accident and Decisions for Remedial Action During the Recovery Phase
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Pereira Jc, Vianna Me, Reis Wg, Godoy Jm, Rochedo Er, Jairo Pinto de Oliveira, Amaral Ec, Campos Mj, and do Rio Ma
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Topsoil ,Waste management ,Epidemiology ,Health, Toxicology and Mutagenesis ,Human decontamination ,Contamination ,Remedial action ,Cesium Radioisotopes ,Accidents ,Soil water ,Radioactive contamination ,Humans ,Soil Pollutants, Radioactive ,Environmental science ,Radiology, Nuclear Medicine and imaging ,Radioisotope Teletherapy ,Brazil ,Decontamination ,Nuclear chemistry ,Recovery phase - Abstract
In September 1987, a powder radioactive source was removed from a teletherapy machine in Goiânia, Brazil. Subsequently, it was ruptured in a residential garden causing the dissemination of 137Cs throughout the city. Soil resuspension processes and burial of contaminated house waste in unused gardens were the major contributors to the Cs dissemination in soils at the secondary contaminated sites. Only locations within a radius of 50 m from the primary contaminated sites presented the need for remedial action. The radiation dose-rate measurements and the soil profiles were good indicators of the extent of the secondary contamination and were fundamental for the decisions taken regarding decontamination procedures. In cases of surface contamination, 60% on average of the total activity remained in the upper 1.5-cm layer over the first 5 mo after the accident, and topsoil removal proved to be an effective procedure for decontamination.
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- 1991
7. Comparative In Vitro Effect of TiF4 to NaF and Potassium Oxalate on Reduction of Dentin Hydraulic Conductance
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Calabria, M, primary, Porfirio, R, primary, Fernandes, S, primary, Wang, L, primary, Buzalaf, M, primary, Pereira, JC, primary, and Magalhães, AC, primary
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- 2014
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8. Efeito Agudo de uma Sessão de Treinamento em Circuito sobre os Níveis da Pressão Arterial e Índice Tornozelo/Braço (Itb) em Mulheres Hipertensas Fisicamente Ativas
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LIMA, RRC, primary, OLIVEIRA, LS, additional, PEREIRA, JC, additional, SILVA, YA, additional, FIGUEIREDO, SANR, additional, LIMA NETO, AJ, additional, and SOUSA, MSC, additional
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- 2013
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9. The Effect of Prophylaxis Method on Microtensile Bond Strength of Indirect Restorations to Dentin
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Soares, CJ, primary, Pereira, JC, primary, Souza, SJB, primary, Menezes, MS, primary, and Armstrong, SR, primary
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- 2012
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10. Effect of Conventional and Resin-modified Glass-Ionomer Liner on Dentin Adhesive Interface of Class I Cavity Walls After Thermocycling
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Sampaio, PCP, primary, de Almeida Júnior, AA, primary, Francisconi, LF, primary, Casas-Apayco, LC, primary, Pereira, JC, primary, Wang, L, primary, and Atta, MT, primary
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- 2011
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11. Supplements with different ruminal undegradable protein levels for dairy heifers in tropical pasture:intake, digestibility and plasma urea nitrogen
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Souffrant, WB, Metges, CC, Oliveira, RL, Pereira, JC, Vieira, RAM, do Nascimento, D, Pacheco, BM, Ribeiro, MD, Leonel, FP, Souffrant, WB, Metges, CC, Oliveira, RL, Pereira, JC, Vieira, RAM, do Nascimento, D, Pacheco, BM, Ribeiro, MD, and Leonel, FP
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The effects of supplements with different levels (high, medium, low) of ruminal undegradable protein (RUP) by Brown-Swiss heifers grazing Brachiaria brizantha cv. Marandu on the intake, digestibility and plasma urea nitrogen concentrations were evaluated. The experiment lasted 90 days, divided in three periods of 30 days, corresponding to May, June and July 2000. Fifteen purebred Brown Swiss heifers (five by treatment), averaging 19.7 months and 394 kg live weight (LW), were used. The pasture availability was monitored to maintain offer of 6% LW, in green dry matter (GDM). The heifers were fed 2.5 kg/head/day of concentrate supplement, offered twice a day. Extrusa was collected in animals fistulated in the esophagus. Indigestible neutral detergent fiber (NDF) in the feeds and feces was used to determine the intake and digestibility. There were no effects of supplements on the pasture, however there were differences among periods, because, as dry season progressed, the forage availability decreased and leaf/colm ratio and senescent material proportion increased. There was no effect of treatments on forage intake or forage + supplement. DM, organic matter (OM) and CP digestibility, and total digestible nutrients (TDN) content were lower for the diets with high RUP content. The animals fed supplement with high RUP content showed average plasma N-urea concentrations lower than those fed supplement with average RUP contents, followed by those fed diets with low RUP.
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- 2003
12. Ruminal degradation kinetics of carob pulp
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Pereira, Jc, González, J, Alvir, Mr, Rodriguez, Cécile, and Revues Inra, Import
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[SDV.SA.ZOO] Life Sciences [q-bio]/Agricultural sciences/Zootechny ,ComputingMilieux_MISCELLANEOUS - Published
- 1994
13. Evaluation of gentamicin first-dose pharmacokinetics in septic critically ill patients: pilot study
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Pereira, JC Gonçalves, primary, Martins, A, additional, and Póvoa, P, additional
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- 2009
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14. Fractionation and in vitro degradation kinetics of extrusa nitrogenous compounds from grazing cattle
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Vieira, RAM, Pereira, JC, Malafaia, PAM, de Queiroz, AC, Goncalves, AL, Vieira, RAM, Pereira, JC, Malafaia, PAM, de Queiroz, AC, and Goncalves, AL
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- 2000
15. Chaos tool implementation for non-singer and singer voice comparison (preliminary study)
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Dajer, Me, primary, Pereira, Jc, additional, and Maciel, Cd, additional
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- 2007
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16. Ruminal degradation kinetics of carob pulp
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Pereira, JC, primary, González, J., additional, Alvir, MR, additional, and Rodriguez, C., additional
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- 1994
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17. Nursing assistance systematization proposal by the NANDA/NIC/NOC taxonomies for the diagnosis of defficient [sic] knowledge.
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Pereira JC, Stuchi RAG, and Arreguy-Sena C
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- 2010
18. Influence of airborne-particle abrasion on mechanical properties and bond strength of carbon/epoxy and glass/bis-GMA fiber-reinforced resin posts.
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Soares CJ, Santana FR, Pereira JC, Araujo TS, and Menezes MS
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STATEMENT OF PROBLEM: Controversy exists concerning the use of fiber-reinforced posts to improve bond strength to resin cement because some precementation treatments can compromise the mechanical properties of the posts. PURPOSE: The purpose of this study was to analyze the influence of airborne-particle abrasion on the mechanical properties and microtensile bond strength (MTBS) of carbon/epoxy and glass/bis-GMA fiber-reinforced resin posts. MATERIAL AND METHODS: Flexural strength (delta(f)), flexural modulus (E(f)), and stiffness (S) were assessed using a 3-point bending test for glass fiber-reinforced and carbon fiber-reinforced resin posts submitted to airborne-particle abrasion (AB) with 50-mum Al(2)O(3), and for posts without any surface treatment (controls) (n=10). Forty glass fiber (GF) and 40 carbon fiber (CF) posts were submitted to 1 of 4 surface treatments (n=10) prior to MTBS testing: silane (S); silane and adhesive (SA); airborne-particle abrasion with 50-mum Al(2)O(3) and silane (ABS); airborne-particle abrasion, silane, and adhesive (ABSA). Two composite resin restorations (Filtek Z250) with rounded depressions in the lateral face were bilaterally fixed to the post with resin cement (RelyX ARC). Next, the specimen was sectioned with a precision saw running perpendicular to the bonded surface to obtain 10 bonded beam specimens with a cross-sectional area of 1 mm(2). Each beam specimen was tested in a mechanical testing machine (EMIC 2000 DL), under stress, at a crosshead speed of 0.5 mm/min until failure. Data were analyzed by 2-way ANOVA followed by Tukey HSD test (alpha=.05). Failure patterns of tested specimens were analyzed using scanning electron microscopy (SEM). RESULTS: The 3-point bending test demonstrated significant differences among groups only for the post type factor for flexural strength, flexural modulus, and stiffness. The carbon fiber posts exhibited significantly higher mean flexural strength (P=.001), flexural modulus (P=.003), and stiffness (P=.001) values when compared with glass fiber posts, irrespective of surface treatment. An alteration in the superficial structure of the posts could be observed by SEM after airborne-particle abrasion. MTBS testing showed no significant effect for the surface treatment type; however, significant effects for post system factor and for interaction between the 2 factors were observed. For the carbon fiber post, the ABSA surface treatment resulted in values significantly lower than the S surface treatment. SEM analysis of MTBS-tested specimens demonstrated adhesive and cohesive failures. CONCLUSIONS: Airborne-particle abrasion did not influence the mechanical properties of the post; however, it produced undesirable surface changes, which could reduce the bond strength to resin cement. For the surface treatments studied, if silane is applied, the adhesive system and airborne-particle abrasion are not necessary. (J Prosthet Dent 2008;99:444-454). [ABSTRACT FROM AUTHOR]
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- 2008
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19. Cardiovascular risk profile and health self-evaluation in Brazil: a population-based study.
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Pereira JC, Barreto SM, and Passos VMA
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OBJECTIVES: To estimate the prevalence and clustering of cardiovascular risk factors (hypertension, smoking, physical inactivity, excessive use of alcohol, inadequate diet, and central obesity) and investigate the association of these factors with health self-evaluation after adjustment for sociodemographic features and clinical diagnosis of diabetes and ischemic heart disease. METHODS: We studied 9 211 Brazilians with age between 30 and 69 years, residents of 16 state capitals, included in a cross-sectional population-based study carried out by the Ministry of Health (Inquérito Domiciliar sobre Comportamentos de Risco e Morbidade Referida de Doenças e Agravos Nao Transmissíveis, 2002-2003). The response variable was the score of cardiovascular risk factor clustering, which ranged from zero to 3: no exposure or exposure to 1, 2, and 3 or more risk factors. Data were analyzed using multinomial logistic regression, having as reference the category of zero risk factors, with and without hypertension. RESULTS: The prevalence of all risk factors except alcohol consumption was higher than 25%. The presence of two or more risk factors occurred in 47.49%; 17.91% of the participants presented zero risk factors. The clustering of risk factors was inversely associated with income and schooling and higher in the age group between 50 and 59 years, without significant differences between sexes. Health self-evaluation was worse in participants with more risk factors. The inclusion of hypertension in the score resulted in a worsening of the risk profile with increase in age and in a significant association with reported ischemic heart disease and diabetes. CONCLUSIONS: The high prevalence of cardiovascular risk factors in Brazilian adults underscores the importance of policies aimed at reducing these factors. The association between worse self-perception of health and a higher number of cardiovascular risk factors calls attention to the importance, broadness and complexity of this subjective health marker in population surveys and suggests that self-perception of poor health is not sufficient to encourage healthy attitudes. [ABSTRACT FROM AUTHOR]
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- 2009
20. Supplements with different ruminal undegradable protein levels for dairy heifers in tropical pasture: intake, digestibility and plasma urea nitrogen
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Oliveira, Rl, Pereira, Jc, Ricardo Vieira, Do Nascimento, D., Pacheco, Bm, Ribeiro, Md, Leonel, Fp, Souffrant, Wb, and Metges, Cc
21. Determination of carbohydrate fractions that constitute the total carbohydrates and ruminal kinetics of neutral detergent fiber in some ruminant feeds
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Pedro Malafaia, Valadares, Sd, Vieira, Ram, Da Silva, Jfc, and Pereira, Jc
22. Determination and ruminal kinetic of protein fractions is some ruminant feeds
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Malafaia, Pam, Valadares, Sd, Ricardo Vieira, Da Silva, Jfc, and Pereira, Jc
23. Ruminal kinetics of some feeds investigated by gravimetric and metabolic techniques
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Malafaia, Pam, Valadares, Sd, Ricardo Vieira, Da Silva, Jfc, and Pereira, Jc
24. Wavelet-based dynamic time warping
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Shi-Huang Chen, Carlos Dias Maciel, Paulo Rogério Scalassara, Rodrigo Capobianco Guido, Sylvio Barbon, José Carlos Pereira, Everthon Silva Fonseca, Lucimar Sasso Vieira, Fabrício Lopes Sanchez, Barbon Junior, S, Guido, Rc, Vieira, L, Fonseca, E, Sanchez, Fl, Scalassara, Pr, Maciel, Cd, Pereira, Jc, and Chen, Sh
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Discrete wavelet transform ,Dynamic time warping ,business.industry ,Applied Mathematics ,Numerical analysis ,Pattern recognition ,Image processing ,Signal ,Computational Mathematics ,ComputingMethodologies_PATTERNRECOGNITION ,Wavelet ,Pattern recognition (psychology) ,Pattern matching ,Artificial intelligence ,business ,Pattern recognition in spoken language ,Mathematics - Abstract
Dynamic Time Warping (DTW), a pattern matching technique traditionally used for restricted vocabulary speech recognition, is based on a temporal alignment of the input signal with the template models. The principal drawback of DTW is its high computational cost as the lengths of the signals increase. This paper shows extended results over our previously published conference paper, which introduces an optimized version of the DTW I hat is based on the Discrete Wavelet Transform (DWT). (C) 2008 Elsevier B.V. All rights reserved.
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- 2009
25. A neural-wavelet architecture for voice conversion
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Rodrigo Capobianco Guido, Fabrício Lopes Sanchez, Lucimar Sasso Vieira, Sylvio Barbon Junior, Carlos Dias Maciel, José Carlos Pereira, Everthon Silva Fonseca, Guido, Rc, Vieira, L, Barbon Junior, S, Sanchez, Fl, Maciel, Cd, Fonseca, E, and Pereira, Jc
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Wavelet ,Artificial Intelligence ,Computer science ,Cognitive Neuroscience ,Speech recognition ,Wavelet transform ,Speech corpus ,TIMIT ,Architecture ,Joint (audio engineering) ,Computer Science Applications ,Wavelet packet decomposition - Abstract
In this letter we propose a new architecture for voice conversion that is based on a joint neural-wavelet approach. We also examine the characteristics of many wavelet families and determine the one that best matches the requirements of the proposed system. The conclusions presented in theory are confirmed in practice with utterances extracted from TIMIT speech corpus. (c) 2007 Elsevier B.V. All rights reserved.
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- 2007
26. Wavelet-based cepstrum calculation
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Rodrigo Capobianco Guido, Carlos Dias Maciel, Paulo Rogério Scalassara, Fabrício Lopes Sanchez, Lucimar Sasso Vieira, José Carlos Pereira, Júnior Sylvio Barbon, Shi-Huang Chen, Everthon Silva Fonseca, Sanchez, Fl, Barbon Junior, S, Vieira, L, Guido, Rc, Fonseca, E, Scalassara, Pr, Maciel, Cd, Pereira, Jc, and Chen, Sh
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business.industry ,Applied Mathematics ,Stationary wavelet transform ,Wavelet transform ,Cascade algorithm ,Pattern recognition ,Speaker recognition ,Pitch period ,Wavelet packet decomposition ,Computational Mathematics ,Wavelet ,ComputingMethodologies_PATTERNRECOGNITION ,Cepstrum ,Speech analysis ,Artificial intelligence ,Mel-frequency cepstrum ,business ,Algorithm ,Mathematics - Abstract
In this paper we present a new wavelet-based algorithm for low-cost computation of the cepstrum. It can be used for real time precise pitch determination in automatic speech and speaker recognition systems. Many wavelet families are examined to determine the one that works best. The results confirm the efficacy and accuracy of the proposed technique for pitch extraction. (C) 2008 Elsevier B.V. All rights reserved.
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- 2009
27. Autoregressive decomposition and pole tracking applied to vocal fold nodule signals
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Fabrício Lopes Sanchez, Rodrigo Capobianco Guido, José Carlos Pereira, Lucimar Sasso Vieira, Paulo Rogério Scalassara, Carlos Dias Maciel, Arlindo Neto Montagnoli, Sylvio Barbon Junior, Everthon Silva Fonseca, Scalasara, Pr, Maciel, Cd, Guido, Rc, Pereira, Jc, Fonseca, E, Montagnoli, An, Barbon Junior, S, Vieira, L, and Sanchez, Fl
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Signal processing ,Autoregressive model ,Artificial Intelligence ,Vocal fold nodule ,Computer science ,Speech recognition ,Signal Processing ,medicine ,Computer Vision and Pattern Recognition ,medicine.disease ,Software ,Frequency spectrum ,Jitter - Abstract
This letter describes a novel algorithm that is based on autoregressive decomposition and pole tracking used to recognize two patterns of speech data: normal voice and disphonic voice caused by nodules. The presented method relates the poles and the peaks of the signal spectrum which represent the periodic components of the voice. The results show that the perturbation contained in the signal is clearly depicted by pole's positions. Their variability is related to jitter and shimmer. The pole dispersion for pathological voices is about 20% higher than for normal voices, therefore, the proposed approach is a more trustworthy measure than the classical ones. (c) 2007 Published by Elsevier B.V.
- Published
- 2007
28. Efficacy and safety of induction chemotherapy in oral cavity cancer: An eight-year experience at a Portuguese reference center.
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Barbosa-Martins J, Rolo A, Lima B, Pereira JC, Araújo C, Guimarães J, Dinis J, and Vieira C
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- Humans, Female, Male, Middle Aged, Retrospective Studies, Aged, Adult, Portugal epidemiology, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Antineoplastic Combined Chemotherapy Protocols adverse effects, Treatment Outcome, Neoplasm Staging, Prognosis, Carcinoma, Squamous Cell drug therapy, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell mortality, Disease-Free Survival, Aged, 80 and over, Induction Chemotherapy methods, Mouth Neoplasms drug therapy, Mouth Neoplasms pathology, Mouth Neoplasms mortality
- Abstract
Background: Induction chemotherapy has been described as an option in locally advanced oral cavity squamous cell carcinoma when the surgical morbidity is expected to be high. This work aimed to evaluate the outcome and safety of induction chemotherapy in this setting., Methods: We performed a retrospective and observational study including patients with oral cavity squamous cell carcinoma, treated with induction chemotherapy between January 2010 and December 2018. Outcomes included induction chemotherapy toxicity, treatment response, disease-free survival and overall survival., Results: A total of 108 oral cavity squamous cell carcinoma patients were included. Ninety-six (88.9%) had stage IV disease, while 12 (11.1%) had stage III. Eighty-four patients (80.8%) achieved at least a partial response to induction chemotherapy at clinical evaluation, and 75 (72.1%) at radiological evaluation. Seventy-eight patients have been proposed for subsequent definitive treatments, with no differences obtained in prognosis, when comparing surgical to non-surgical approaches. In patients treated with definitive treatments, improved five-year disease-free survival was obtained if at least a clinical (56.3%; p=0.001) or radiological (52.9%; p=0.001) partial response was achieved after induction chemotherapy. Similarly, superior five-year overall survival was verified for those achieving at least clinical (51.1%; p<0.0001) or radiological (52.6%; p=0.001) partial response. Also, accomplishing a pathologic complete response (n=22.6%) significantly improved disease-free survival (p=0.039) and overall survival (p=0.005). Grade 3 and 4 toxicities were observed in 52 patients (41.8%)., Conclusion: Responses to induction chemotherapy predicted prognosis in our population, however important toxicities were observed. Further studies are necessary to identify induction chemotherapy response predictors and subgroups who may benefit from this approach., Competing Interests: Declaration of conflicting interestThe author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: JBM: Honoraria: Roche, Pierre Fabre. The remaining authors declare no conflict of interest.
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- 2024
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29. Continuous monitoring after laparoscopic Roux-En-Y gastric bypass: a pathway to ambulatory care surgery - a pilot study.
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Ferreira-Santos R, Pinto JP, Pinho JP, Ribeiro AC, da Costa M, Vieira V, Ferreira C, Manso F, and Pereira JC
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Same-day discharge (SDD) after Laparoscopic Roux-En-Y Gastric Bypass (LRYGB) faces resistance due to possible undetected postoperative complications. These present with changes in vital signs, which continuous remote monitoring devices can detect. This study compared continuous vital signs monitoring using the Isansys Patient Status Engine™ with standard nursing vital signs measurements to assess the device's reliability in postoperative surveillance of patients undergoing LRYGB. We conducted a pilot study including patients who underwent LRYGB. During their hospital stay, patients were continuously monitored using the Isansys Patient Status Engine™ with Lifetouch™, Lifetemp™, and Nonin Pulse Oximeter™ sensors. The heart rate (HR), body temperature, and oxygen saturation (SpO
2 ) collected by the device were compared with standard nursing assessments. Thirteen patients with a mean body mass index of 41.5 ± 4.4 kg/m2 were included. No major complications occurred. The median HR assessed by standard and continuous monitoring did not significantly differ (75.5 [69-88] vs. 77 [66-91] bpm, p = 0.995), nor did the mean values of SpO2 (94.7 ± 2.0 vs. 93.7 ± 1.8%, p = 0,057). A significant difference was observed in median body temperature between the nursing staff and the monitoring device (36.3 [36.1-36.7] vs. 36.1 [34.5-36.6] degrees Celsius, p = 0.012), with a tendency for lower temperature measurements by the device. In conclusion, this is the first study on continuous postoperative surveillance using the Isansys Patient Status Engine™ monitoring device for LRYGB patients. Our results introduce a novel tool for more efficient surgery. Prospective randomized experimental studies are warranted to evaluate this method's efficacy and safety., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)- Published
- 2024
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30. Estimating sequence diversity of prion protein gene ( PRNP ) in Portuguese populations of two cervid species: red deer and fallow deer.
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Pereira JC, Gonçalves-Anjo N, Orge L, Pires MA, Rocha S, Figueira L, Matos AC, Silva J, Mendonça P, Carvalho P, Tavares P, Lima C, Alves A, Esteves A, Pinto ML, Pires I, Gama A, Sargo R, Silva F, Seixas F, Vieira-Pinto M, and Bastos E
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- Animals, Prion Proteins genetics, Portugal, Prions genetics, Deer genetics, Prion Diseases veterinary, Wasting Disease, Chronic genetics, Wasting Disease, Chronic metabolism
- Abstract
Among the transmissible spongiform encephalopathies (TSEs), chronic wasting disease (CWD) in cervids is now a rising concern in wildlife within Europe, after the detection of the first case in Norway in 2016, in a wild reindeer and until June 2022 a total of 34 cases were described in Norway, Sweden and Finland. The definite diagnosis is post-mortem , performed in target areas of the brain and lymph nodes. Samples are first screened using a rapid test and, if positive, confirmed by immunohistochemistry and Western immunoblotting. The study of the genetics of the prion protein gene, PRNP , has been proved to be a valuable tool for determining the relative susceptibility to TSEs. In the present study, the exon 3 of PRNP gene of 143 samples from red deer ( Cervus elaphus ) and fallow deer ( Dama dama ) of Portugal was analysed. Three single nucleotide polymorphisms (SNPs) were found in red deer - codon A136A, codon T98A, codon Q226E - and no sequence variation was detected in fallow deer. The low genetic diversity found in our samples is compatible with previous studies in Europe. The comparison with results from North America suggests that the free-ranging deer from our study may present susceptibility to CWD, although lack of experimental data and the necessity of continuous survey are necessary to evaluate these populations.
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- 2023
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31. Integration of fluorescence in situ hybridization and chromosome-length genome assemblies revealed synteny map for guinea pig, naked mole-rat, and human.
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Romanenko SA, Kliver SF, Serdyukova NA, Perelman PL, Trifonov VA, Seluanov A, Gorbunova V, Azpurua J, Pereira JC, Ferguson-Smith MA, and Graphodatsky AS
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- Humans, Guinea Pigs, Animals, Synteny, In Situ Hybridization, Fluorescence, Karyotype, Genome, Mole Rats genetics
- Abstract
Descriptions of karyotypes of many animal species are currently available. In addition, there has been a significant increase in the number of sequenced genomes and an ever-improving quality of genome assembly. To close the gap between genomic and cytogenetic data we applied fluorescent in situ hybridization (FISH) and Hi-C technology to make the first full chromosome-level genome comparison of the guinea pig (Cavia porcellus), naked mole-rat (Heterocephalus glaber), and human. Comparative chromosome maps obtained by FISH with chromosome-specific probes link genomic scaffolds to individual chromosomes and orient them relative to centromeres and heterochromatic blocks. Hi-C assembly made it possible to close all gaps on the comparative maps and to reveal additional rearrangements that distinguish the karyotypes of the three species. As a result, we integrated the bioinformatic and cytogenetic data and adjusted the previous comparative maps and genome assemblies of the guinea pig, naked mole-rat, and human. Syntenic associations in the two hystricomorphs indicate features of their putative ancestral karyotype. We postulate that the two approaches applied in this study complement one another and provide complete information about the organization of these genomes at the chromosome level., (© 2023. The Author(s).)
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- 2023
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32. Buthionine sulfoximine and chemoresistance in cancer treatments: a systematic review with meta-analysis of preclinical studies.
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Dos Reis Oliveira C, Pereira JC, Barros Ibiapina A, Roseno Martins IR, de Castro E Sousa JM, Ferreira PMP, and Carneiro da Silva FC
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- Humans, Buthionine Sulfoximine pharmacology, Buthionine Sulfoximine therapeutic use, Methionine Sulfoximine therapeutic use, Methionine Sulfoximine toxicity, Drug Resistance, Neoplasm, Neoplasms drug therapy, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use
- Abstract
Buthionine sulfoximine (BSO) is a synthetic amino acid that blocks the biosynthesis of reduced glutathione (GSH), an endogenous antioxidant cellular component present in tumor cells. GSH levels have been associated with tumor cell resistance to chemotherapeutic drugs and platinum compounds. Consequently, by depleting GSH, BSO enhances the cytotoxicity of chemotherapeutic agents in drug-resistant tumors. Therefore, the aim of this study was to conduct a systematic review with meta-analysis of preclinical studies utilizing BSO in cancer treatments. The systematic search was carried out using the following databases: PubMed, Web of Science, Scopus, and EMBASE up until March 20, 2023, in order to collect preclinical studies that evaluated BSO, alone or in association, as a strategy for antineoplastic therapy. One hundred nine investigations were found to assess the cytotoxic potential of BSO alone or in combination with other compounds. Twenty-one of these met the criteria for performing the meta-analysis. The evidence gathered indicated that BSO alone exhibits cytotoxic activity. However, this compound is generally used in combination with other antineoplastic strategies, mainly chemotherapy ones, to improve cytotoxicity to carcinogenic cells and treatment efficacy. Finally, this review provides important considerations regarding BSO use in cancer treatment conditions, which might optimize future studies as a potential adjuvant antineoplastic therapeutic tool.
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- 2023
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33. Chromosome evolution in Iberolacerta, a genus that deviates from the standard karyotype formula of Lacertidae.
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Naveira H, Rojo V, Gómez-Seoane I, Ferguson-Smith MA, Pereira JC, and Martínez-Lage A
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- Animals, Comparative Genomic Hybridization, Karyotyping, Karyotype, Evolution, Molecular, Sex Chromosomes genetics, Lizards genetics
- Abstract
This paper describes the preparation of flow-sorted chromosome paints from the Iberian Rock lizard Iberolacerta monticola, exemplifying their subsequent use in cross-species comparisons of chromosome painting. We carried out comparative analyses of chromosome evolution in the congeneric species I. galani and I. bonnali, as well as in two other species of Lacertini (Lacerta schreiberi and Timon lepidus) whose sex chromosomes were also studied through comparative genomic hybridization. Most species of Lacertini possess a diplod number of 2n = 38, with 36 acrocentric macrochromosomes and 2 microchromosomes. However, the nine species included in the genus Iberolacerta do not possess microchromosomes. Furthermore, very conspicuous differences from the standard Lacertini karyotype were observed in the three Pyrenean species of this genus, which included several biarmed metacentrics and a Z
1 Z2 W multiple sex-chromosome system. With the possible exception of L. schreiberi, all the species of the family Lacertidae described to date appear to share homologous Z chromosomes, which date back to the last common ancestor of the whole group. We provide conclusive evidence that L. schreiberi should no longer be considered an exception to this rule, and demonstrate that the loss of microchromosomes in Iberolacerta was produced by their fusion to a middle-sized chromosome. Furthermore, we show that the multiple sex-chromosome system of the Pyrenean species of Iberolacerta originated from the fusion of the ancestral W chromosome with one of the shortest autosomes, and provide additional evidence of the fast evolution of DNA sequences linked to the W chromosome in Lacertini., (© 2023. The Author(s).)- Published
- 2023
- Full Text
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34. Systematic review of population-based bladder cancer registries: How criteria heterogeneity affects the comparison of incidences.
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Caballero JM, Gili JM, Pereira JC, Gomáriz A, Castillo C, and Martín-Baranera M
- Subjects
- Humans, Incidence, Registries, Longevity, SEER Program, Urinary Bladder Neoplasms epidemiology, Carcinoma in Situ
- Abstract
Background: The population-based registry of bladder cancer (BC) raises specific problems intrinsic to the tumor, as the inclusion of noninfiltrating, potentially malignant and multiple tumors. We performed a systematic review (PRISMA guidelines) of population-based BC registries to obtain information on their geographic areas involved, last dates of real incidence of BC, and rules coding used in BC for uncertain behavior, in situ and multiple tumors., Methods: Using MEDLINE and Google Scholar, we identified scientific publications of in the last 10 years in English or Spanish, whether they were related to a national or international cancer registry, provided information on registry rules, and provided data on the incidence of BC., Results: After the first screening, a total of 194 references were obtained. After a second analysis, three registries were selected: International Agency for Research on Cancer (IARC) is a world registry providing real incidence of BC in the period 2008-2012. Surveillance, Epidemiology, and End Results (SEER) Program registered incidence until 2017 in more than 90% of the US population. Spanish Network of Cancer Registries (REDECAN) unifies 14 Spanish registries (27.4% of the population) with real incidence data from 2010 to 2015. The coding and inclusion rules have been modified, but currently, most registries include BC in situ and uncertain behavior tumors. Whenever a new case occurs 36 months after a previous diagnosis, SEER registers those as multiple incident cancers in the same location, while IARC and REDECAN only allow one cancer per location during the lifespan of the patient., Conclusions: Comparison of the incidence of BC among different population-based cancer registries is prone to bias due to the methodological differences regarding the inclusion of carcinomas in situ, indeterminate, and multiple tumors. A good cancer registry could provide better surveillance strategies for BC patients., (© 2022 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
- Published
- 2023
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35. Risk Factors Involved in the High Incidence of Bladder Cancer in an Industrialized Area in North-Eastern Spain: A Case-Control Study.
- Author
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Caballero JM, Gili JM, Pereira JC, Gomáriz A, Castillo C, and Martín-Baranera M
- Abstract
Bladder cancer (BC) is the most common of the malignancies affecting the urinary tract. Smoking and exposure to occupational and environmental carcinogens are responsible for most cases. Vallès Occidental is a highly industrialized area in north-eastern Spain with one of the highest incidences of BC in men. We carried out a case-control study in order to identify the specific risk factors involved in this area. Three hundred and six participants were included (153 cases BC and 153 controls matched for age and sex): in each group, 89.5% ( n = 137) were male and the mean age was 71 years (range 30-91; SD = 10.6). There were no differences between groups in family history, body mass index, or dietary habits. Independent risk factors for CV were smoking (OR 2.08; 95% CI 1.30-3.32; p = 0.002), the use of analgesics in nonsmokers (OR 10.00; 95% CI 1.28-78.12; p = 0.028), and profession (OR: 8.63; 95% CI 1.04-71.94; p = 0.046). The consumption of black and blond tobacco, the use of analgesics in nonsmokers, and occupational exposures are risk factors for the development of BC in this area, despite the reduction in smoking in the population and the extensive measures taken in the last few decades in major industries to prevent exposure to occupational carcinogens.
- Published
- 2023
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36. Right colon cancer: The influence of specific location on recurrence and survival.
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Cavadas AS, Gonçalves E, Pereira CC, Rodrigues J, and Pereira JC
- Subjects
- Humans, Female, Male, Retrospective Studies, Longitudinal Studies, Prognosis, Colonic Neoplasms surgery, Colon, Transverse surgery, Colon, Transverse pathology
- Abstract
Aim: This study aimed to investigate whether the site of the tumour within the right colon affects survival in patients who underwent right colectomy for colon cancer., Methods: An observational retrospective longitudinal study was performed in patients who underwent right colectomy for non-metastatic, invasive right-sided colon cancer. Patients were categorized into two groups based on tumour location: (i) caecum and ascending colon; (ii) hepatic flexure and proximal transverse colon. Demographic and clinical features were characterized, and a survival analysis was performed., Results: Of the 198 patients enroled in the study, 134 (67.8%) had caecal or ascending colon cancer and 64 (32.3%) had hepatic flexure or transverse colon cancer. Seventy (35.4%) were female and the mean age at the time of surgery was 71.6 (SD 11.4). The groups were comparable with respect to the number of lymph nodes sampled, the pTNM stage, the histological differentiation grade and the likelihood of patients receiving adjuvant chemotherapy. Recurrence rate was nearly twice as high in the hepatic flexure and proximal transverse colon group (12.5% vs 6.7%), but this difference was not statistically significant (p = 0.174). Kaplan-Meier analysis showed no differences in disease-free (p = 0.255) and overall survival (p = 0.258) between the groups., Conclusion: In our population, specific location of right-sided colon cancers does not appear to have an influence on survival. Further investigation is needed to determine if tumour subsite has an impact on the recurrence rate, and whether it should be considered in defining prognosis and treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper, (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
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37. Hospital-acquired pneumonia is more frequent and lethal in stroke patients: A nationwide 4-year study.
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Gonçalves-Pereira JC, Marino F, Mergulhão P, Nunes B, and Froes F
- Subjects
- Humans, Hospitalization, Incidence, Hospitals, Risk Factors, Healthcare-Associated Pneumonia epidemiology, Stroke epidemiology, Pneumonia epidemiology, Cross Infection epidemiology
- Abstract
We report a higher incidence of hospital-acquired pneumonia (HAP) in patients admitted with stroke (odds ratio, 5.6; 95% CI, 5.4-5.8). Patients with HAP and stroke had an elevated risk of death (odds ratio, 1.2; 95% CI, 1.1-1.3). The incidence and mortality of HAP in stroke patients increased across all age groups.
- Published
- 2023
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38. Letter to the Editor: Comment on "Prediction of Survival Outcomes Based on Preoperative Clinical Parameters in Gastric Cancer".
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Bektaş M, Pereira JC, and van der Peet DL
- Subjects
- Humans, Weight Loss, Stomach Neoplasms surgery, Obesity, Morbid surgery
- Published
- 2022
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39. Observations on chromosome-specific sequencing for the construction of cross-species chromosome homology maps and its resolution of human:alpaca homology.
- Author
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Ferguson-Smith MA, Pereira JC, Borges A, and Kasai F
- Abstract
Background: The history of comparative chromosome mapping is briefly reviewed, with discussion about the problem that occurs in chromosome painting when size heteromorphisms between homologues cause contamination in chromosomes sorted by flow cytometry that are used in the preparation of chromosome-specific DNA probes., Main Body: As an example, we show in the alpaca (Vicagna pacos) that sequencing of contaminated chromosome sorts can reveal chromosome homologies from alignment with human and mouse genome reference sequences. The procedure identifies syntenic blocks of DNA separated in the human karyotype that are associated in the closely related alpaca and dromedary (Camelus dromedarius) karyotypes. This example provides proof of principal for the validity of the method for comparative chromosome mapping., Conclusion: It is suggested that the approach presented here may have application in the construction of comparative chromosome maps between distantly related taxa, such as monotremes and mammals., (© 2022. The Author(s).)
- Published
- 2022
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40. Laparoscopic and endoscopic cooperative surgery for full-thickness resection and sentinel node dissection for early gastric cancer.
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Vanella S, Godas M, Pereira JC, Pereira A, Apicella I, and Crafa F
- Abstract
The endoscopic submucosal dissection (ESD) technique has become the gold standard for submucosal tumors that have negligible risk of lymph node metastasis (LNM), due to its minimal invasiveness and ability to improve quality of life. However, this technique is limited in stage T1 cancers that have a low risk of LNM. Endoscopic full thickness resection can be achieved with laparoscopic endoscopic cooperative surgery (LECS), which combines laparoscopic gastric wall resection and ESD. In LECS, the surgical margins from the tumor are clearly achieved while performing organ-preserving surgery. To overcome the limitation of classical LECS, namely the opening of the gastric wall during the procedure, which increases the risk of peritoneal tumor seeding, non-exposed endoscopic wall-inversion surgery was developed. With this full-thickness resection technique, contact between the intra-abdominal space and the intragastric space was eliminated., Competing Interests: Conflict-of-interest statement: All the authors declare no conflicts of interest., (©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.)
- Published
- 2022
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41. Artificial Intelligence in Bariatric Surgery: Current Status and Future Perspectives.
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Bektaş M, Reiber BMM, Pereira JC, Burchell GL, and van der Peet DL
- Subjects
- Algorithms, Artificial Intelligence, Humans, Weight Loss, Bariatric Surgery, Obesity, Morbid surgery
- Abstract
Background: Machine learning (ML) has been successful in several fields of healthcare, however the use of ML within bariatric surgery seems to be limited. In this systematic review, an overview of ML applications within bariatric surgery is provided., Methods: The databases PubMed, EMBASE, Cochrane, and Web of Science were searched for articles describing ML in bariatric surgery. The Cochrane risk of bias tool and the PROBAST tool were used to evaluate the methodological quality of included studies., Results: The majority of applied ML algorithms predicted postoperative complications and weight loss with accuracies up to 98%., Conclusions: In conclusion, ML algorithms have shown promising capabilities in the prediction of surgical outcomes after bariatric surgery. Nevertheless, the clinical introduction of ML is dependent upon the external validation of ML., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
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42. Environmental Factors Involved in the High Incidence of Bladder Cancer in an Industrialized Area in North-Eastern Spain.
- Author
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Caballero JM, Pérez-Márquez M, Gili JM, Pereira JC, Gomáriz A, Castillo C, and Martín-Baranera M
- Subjects
- Aged, Aged, 80 and over, Carcinogens analysis, Environmental Exposure adverse effects, Environmental Exposure analysis, Female, Humans, Incidence, Male, Middle Aged, Particulate Matter analysis, Spain epidemiology, Air Pollutants analysis, Air Pollution adverse effects, Environmental Pollutants analysis, Urinary Bladder Neoplasms chemically induced, Urinary Bladder Neoplasms etiology
- Abstract
Background: Bladder cancer (BC) is the most common of those affecting the urinary tract, and a significant proportion of the cases are attributable to tobacco use as well as occupational and environmental factors., Objective: The aim of this study is to estimate the current incidence of BC in an industrialized area in northeastern Spain and to analyze its time trends over three decades from an ecological perspective., Methods: Patients diagnosed with histologically confirmed primary BC, during 2018-2019, in an area in northeastern Spain (430,883 inhabitants) were included. Crude and age-standardized incidence rates were estimated per 100,000 person-years based on the number of individuals getting their first diagnosis. An exploratory time trend analysis was carried out to describe the evolution in tobacco use and occupational or environmental risk factors and the incidence of BC in the same area from the 1990s., Results: 295 patients were included (age 72.5 ± 10.3 years; 89.8% men). The crude rate was 62.6 (95% CI: 51.9-73.2) for men and 6.8 (95% CI: 3.4-10.3) for women. The annual rate adjusted to the European Standard Population was 85.3 (95% CI:75.0-95.5) for men and 7.0 (95% CI:4.5-9.5) for women. From 1994 to 2018, the prevalence of smokers decreased in men (42.3% to 30.9%) as well as in the active population working in the industry (44.36% to 22.59%). Nevertheless, the car fleet, especially diesel, has increased considerably. The annual mean concentrations of air (PM
10 , PM2.5 , O3 , and NO2 ) and water (nitrates, arsenic, trihalomethanes) pollutants were within the regulatory limit values, but not the maximum levels., Conclusions: The incidence of BC is one of the highest in men but not in women, despite the decrease in tobacco use and industrial activity (perhaps related to high latency after carcinogen exposure cessation) and despite the control of environmental pollution (the maximum regulatory limit probably needs to be lowered). Finally, a similar exposure to the carcinogen would result in a gender-specific differential incidence., Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper., (Copyright © 2022 José M. Caballero et al.)- Published
- 2022
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43. Application of a recombinant GH10 endoxylanase from Thermoascus aurantiacus for xylooligosaccharide production from sugarcane bagasse and probiotic bacterial growth.
- Author
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Nascimento CEO, Simões LCO, Pereira JC, da Silva RR, de Lima EA, de Almeida GC, Penna ALB, Boscolo M, Gomes E, and da Silva R
- Subjects
- Cellulose, Endo-1,4-beta Xylanases genetics, Glucuronates, Hydrolysis, Oligosaccharides, Xylans, Probiotics, Saccharum, Thermoascus
- Abstract
Xylooligosaccharides (XOs) are a promising class of prebiotics capable of selectively stimulating the growth of the beneficial intestinal microbiota against intestinal pathogens. They can be obtained from xylan present in residual lignocellulosic material from agriculture. Thus, in this study we produced XOs by extracting xylan from sugarcane bagasse and hydrolyzing it using the GH10 xylanase from Thermoascus aurantiacus expressed by Pichia pastoris. An alkaline method to extract xylan is described, which resulted in 83.40% of xylan recovery and low amounts of cellulose and lignin. The enzymatic hydrolysate exhibited a mixture of XOs containing mainly xylobiose, xylotriose and xylotetraose. These oligosaccharides stimulated the growth of Lactobacillus casei, L. rhamnosus, L. fermentum and L. bulgaricus strains, which were able to produce organic acids, especially acetic acid. These findings demonstrate the possibility to redirect crop by-products to produce XOs and their use as a supplement to stimulate the growth of probiotic strains., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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44. In vitro nematocidal activity of Punica granatum L. against gastrointestinal helminths in goats.
- Author
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da Silva Felix RC, Barbosa TN, Marques HP, de Oliveira Rebouças CK, da Silveira Pereira JC, Batista JIL, de Paiva Soares KM, da Silva MDC, and Bezerra ACDS
- Abstract
The objective of this study was to evaluate the in vitro ovicidal activity, phytochemistry, and toxicity of a saline extract obtained from peel of Punica granatum L fruits. The ovicidal activity was evaluated by the hatching inhibition of eggs recovered from fecal samples of naturally infected goats; the phytochemical analysis was carried out using the fruit peel; and the toxicity was tested on Artemia salina , using saline extract. The results showed that the ovicidal effect of the tested extract was 99% (25 mg mL
-1 ), 99% (12.5 mg mL-1 ), 98% (6.25 mg mL-1 ), and 95% (3.12 mg mL-1 ), higher than that of the control drug, thiabendazole (83%). The phytochemical analysis showed presence of phenols, anthraquinones, and condensed and hydrolysable tannins in the fruit extract. The toxicity test of the extract of P. granatum showed an LC50 of 6.19 mg mL-1 , which indicates a safe use for a concentration of 3.12 mg mL-1 , since it was the tested concentration that was below the reliable LC50. The saline extract from peels of P. granatum has ovicidal activity, important secondary metabolites, and absence of toxicity at the lowest concentration tested. However, in vivo tests in experimental models are recommended before performing experiments in ruminants., Competing Interests: Conflict of interestThe authors have no relevant financial or non-financial interests to disclose., (© Indian Society for Parasitology 2021.)- Published
- 2022
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45. A Method for Determination of the Transmission Efficiency of a Silica Optical Fiber Cable Using a Solar Power Tower.
- Author
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Rosa LG, De Almeida G, Garcia Pereira JC, Martínez-Hernández A, and González-Aguilar J
- Abstract
After being adequately captured and concentrated, solar radiation can be conducted by optical fiber bundles/cables and directly used for illumination (lighting) or heating of confined spaces, or indirectly used by converting it in other forms of energy (e.g., for producing electricity). This article reports preliminary tests conducted on a 7-m-long optical fiber bundle/cable with an effective aperture circular area of 14 mm in diameter, specially designed and manufactured by a leading company to transmit up to 1000 W
th of unfiltered concentrated sunlight. The cable was tested in the typical receiver position at the top of a solar concentration central tower. The main purpose was the experimental determination of the transmission efficiency of the cable in function of the incidence angle using selected groups of heliostats belonging to the heliostat field. The testing methodology proved to be capable of evaluating the performance of the cable. The cable withstood the tests without revealing any type of damage. The results obtained showed that the transmissivity of the cable is higher than 50% when the incidence angle of the solar radiation is lower than 14.7°, increasing sharply to circa 95% when the incidence angle is lower than 4.5°.- Published
- 2022
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46. Whole-chromosome fusions in the karyotype evolution of Sceloporus (Iguania, Reptilia) are more frequent in sex chromosomes than autosomes.
- Author
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Lisachov AP, Tishakova KV, Romanenko SA, Molodtseva AS, Prokopov DY, Pereira JC, Ferguson-Smith MA, Borodin PM, and Trifonov VA
- Subjects
- Animals, Male, Synaptonemal Complex genetics, Biological Evolution, Karyotype, Lizards genetics, Sex Chromosomes genetics
- Abstract
Whole-chromosome fusions play a major role in the karyotypic evolution of reptiles. It has been suggested that certain chromosomes tend to fuse with sex chromosomes more frequently than others. However, the comparative genomic synteny data are too scarce to draw strong conclusions. We obtained and sequenced chromosome-specific DNA pools of Sceloporus malachiticus , an iguanian species which has experienced many chromosome fusions. We found that four of seven lineage-specific fusions involved sex chromosomes, and that certain syntenic blocks which constitute the sex chromosomes, such as the homologues of the Anolis carolinensis chromosomes 11 and 16, are repeatedly involved in sex chromosome formation in different squamate species. To test the hypothesis that the karyotypic shift could be associated with changes in recombination patterns, we performed a synaptonemal complex analysis in this species and in Sceloporus variabilis (2 n = 34). It revealed that the sex chromosomes in S. malachiticus had two distal pseudoautosomal regions and a medial differentiated region. We found that multiple fusions little affected the recombination rate in S. malachiticus . Our data confirm more frequent involvement of certain chromosomes in sex chromosome formation, but do not reveal a connection between the gonosome-autosome fusions and the evolution of recombination rate. This article is part of the theme issue 'Challenging the paradigm in sex chromosome evolution: empirical and theoretical insights with a focus on vertebrates (Part II)'.
- Published
- 2021
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47. Growth resistance and resilience of mixed silver fir and Norway spruce forests in central Europe: Contrasting responses to mild and severe droughts.
- Author
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Bottero A, Forrester DI, Cailleret M, Kohnle U, Gessler A, Michel D, Bose AK, Bauhus J, Bugmann H, Cuntz M, Gillerot L, Hanewinkel M, Lévesque M, Ryder J, Sainte-Marie J, Schwarz J, Yousefpour R, Zamora-Pereira JC, and Rigling A
- Subjects
- Climate Change, Europe, Forests, Norway, Droughts, Ecosystem
- Abstract
Extreme droughts are expected to increase in frequency and severity in many regions of the world, threatening multiple ecosystem services provided by forests. Effective strategies to adapt forests to such droughts require comprehensive information on the effects and importance of the factors influencing forest resistance and resilience. We used a unique combination of inventory and dendrochronological data from a long-term (>30 years) silvicultural experiment in mixed silver fir and Norway spruce mountain forests along a temperature and precipitation gradient in southwestern Germany. We aimed at examining the mechanisms and forest stand characteristics underpinning the resistance and resilience to past mild and severe droughts. We found that (i) fir benefited from mild droughts and showed higher resistance (i.e., lower growth loss during drought) and resilience (i.e., faster return to pre-drought growth levels) than spruce to all droughts; (ii) species identity determined mild drought responses while species interactions and management-related factors strongly influenced the responses to severe droughts; (iii) intraspecific and interspecific interactions had contrasting effects on the two species, with spruce being less resistant to severe droughts when exposed to interaction with fir and beech; (iv) higher values of residual stand basal area following thinning were associated with lower resistance and resilience to severe droughts; and (v) larger trees were resilient to mild drought events but highly vulnerable to severe droughts. Our study provides an analytical approach for examining the effects of different factors on individual tree- and stand-level drought response. The forests investigated here were to a certain extent resilient to mild droughts, and even benefited from such conditions, but were strongly affected by severe droughts. Lastly, negative effects of severe droughts can be reduced through modifying species composition, tree size distribution and stand density in mixed silver fir-Norway spruce forests., (© 2021 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)
- Published
- 2021
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48. Enhanced recovery after surgery (ERAS ® ) protocol adapted to the Brazilian reality: a prospective cohort study for thoracic patients.
- Author
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Abrão FC, Araujo de França S, de Abreu IRLB, das Neves Pereira JC, Del Massa EC, Oliver A, and Cavalcante MGC
- Abstract
Background: In Low-Middle Income Countries (LMICs), resource optimization and infrastructure availability are recurrently in debate. In order to assist the development and implementation of guidelines, LMICs often exemplify from High-Income Countries protocols. At the final, it will be: content adaption is often needed. In this study, we demonstrated the preliminary analysis of the Brazilian experience by adapting the ERAS
® Protocol for thoracic surgery patients (PROSM)., Methods: Patients' data were extracted from the surgical group database that operated in the city of Sao Paulo. Patients' data were organized for analysis after the institution's ethics committee gave their approval. Patients' variables were analyzed and compared to a control group. Subgroup analysis included patients without ICU Admission., Results: PROSM patients had reduced ICU length of stay (LOS) (Mean of 0.3±0.58 days, 1.2±1.65 days, P=0.001), Hospital LOS (Mean of 1.6±1.32 days, 3.9±3.25 days, P=0.001) and Chest Drain duration (Median 1.0±1.00 days, 3.0±3.00 days, P=0.001). Analyses of patients that were not admitted to the ICU demonstrated reduced Hospital LOS and Chest drain duration. Cost analysis, such as procedure, daily, and post-surgical costs were also significantly lower towards PROSM group., Conclusions: This study revealed important aspects for improvement of the delivered care quality and opportunity for expenditure management. We expect to assist more countries to improve knowledge under the implementation of enhanced protocols., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://dx.doi.org/10.21037/jtd-21-920). The authors have no conflicts of interest to declare., (2021 Journal of Thoracic Disease. All rights reserved.)- Published
- 2021
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49. Obliterating potential of active products for dentin hypersensitivity treatment under an erosive challenge.
- Author
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Francisconi-Dos-Rios LF, Dantas LM, Calabria MP, Pereira JC, Mosquim V, and Wang L
- Subjects
- Dentin, Dentin Permeability, Humans, Microscopy, Electron, Scanning, Dentin Desensitizing Agents pharmacology, Dentin Desensitizing Agents therapeutic use, Dentin Sensitivity drug therapy
- Abstract
Objectives: To compare the effect of 5 desensitizing agents on the hydraulic conductance (Lp) of dentin and to analyze its surface under a Laser Scanning Confocal Microscope (LSCM) before and after an erosive challenge., Methods: Lp was analyzed in the following sequence: in the presence of smear layer (pMin), after a 15-second acid etching (pMax), after treatment with a desensitizing agent (pTreat) and after a 1-minute erosive challenge (6% citric acid - pEro). Fifty 1.0 ± 0.2 mm-thick dentin disks were prepared from sound human third molars and were randomly distributed into 5 groups (n = 10): FG-Fluoride gel (control), SA-Sensiactive, PR-Sensitive Pro-Relief, NP-Desensibilize Nano-P and EV-Enamel Pro Varnish. Data were analyzed by two-way ANOVA and Tukey tests (α<0.05). Additional specimens were analyzed under a Laser Scanning Confocal Microscope (LSCM) and by Energy Dispersive X-ray Spectroscopy (EDS)., Results: All materials reduced the Lp in some extent, except for FG and EV. After the erosive challenge, SA was the only material effectively able to keep the same Lp of the post-treatment phase, while the other products showed lower resistance upon an erosive challenge. Under the LSCM, the SA and NP materials were more effective to obliterate the opened dentin tubules and demonstrated higher resistance upon an erosive challenge. The EDS analysis evidenced levels of Ca, O, P, Si, Na and S., Conclusions: Product containing potassium oxalate was the most effective in reducing Lp of dentin samples before and after an erosive challenge. Under a LSCM, products containing potassium oxalate or hydroxyapatite crystals seemed to be occluding the dentin tubules after an erosive challenge., (Copyright © 2021. Published by Elsevier Ltd.)
- Published
- 2021
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50. Removal of Imidacloprid from Water by Microalgae Nannochloropsis sp. and Its Determination by a Validated RP-HPLC Method.
- Author
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Encarnação T, Santos D, Ferreira S, Valente AJM, Pereira JC, Campos MG, Burrows HD, and Pais AACC
- Subjects
- Animals, Bees, Chromatography, High Pressure Liquid, Neonicotinoids, Nitro Compounds, Wastewater, Water, Microalgae, Stramenopiles
- Abstract
The large-scale use of pesticides is one of the main causes of the dramatic degradation of our environment. Pesticides such as imidacloprid (IMID) have been linked to declines in bee health and toxicity to other beneficial insects. They pose a threat to human health due to their persistence in the environment and accumulation in the food chain. Therefore, it is essential to test possible environmentally-friendly solutions for their elimination. The present study evaluates the efficiency of microalgae Nannochloropsis sp. for the removal of IMID from synthetic wastewater. The influence of aeration, light, and the presence of UV radiation on the degradation of IMID were factors considered in the study. A rapid RP-HPLC method was developed and validated for the analysis and quantification of IMID in the context of bioremediation with microalgae. Nannochloropsis sp. removed 4.39 µg mL
-1 from an initial content of 9.59 µg mL-1 (reaching approximately 50%) of IMID in the first 20 h. This study demonstrated that the removal of IMID by the marine microalgae Nannochloropsis sp. is both effective and light-dependent., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2021
- Full Text
- View/download PDF
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