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14 results on '"Pereira, Welbert Oliveira"'

2. AKT inhibition interferes with the expression of immune checkpoint proteins and increases NK-induced killing of HL60-AML cells

Author

Gama, Sofia Mônaco, Varela, Vanessa Araújo, Ribeiro, Natalia Mazini, Bizzarro, Bruna, Hernandes, Camila, Aloia, Thiago Pinheiro Arrais, Amano, Mariane Tami, and Pereira, Welbert Oliveira

Subjects
PD-L1, Gal-9, Killer cells, natural, Leukemia, myeloid, acute, AKT
Abstract

Objective To determine the role of the AKT pathway in the regulating of natural Killer-induced apoptosis of acute myeloid leukemia cells and to characterize the associated molecular mechanisms. Methods BALB/c nude mice were injected with HL60 cells to induce a xenogenic model of subcutaneous leukemic tumors. Mice were treated with perifosine, and their spleens were analyzed using biometry, histopathology, and immunohistochemistry. Gene expression analysis in leukemia cells was performed by real-time PCR. Protein analysis of leukemia and natural Killer cells was performed by flow cytometry. AKT inhibition in HL60 cells, followed by co-culture with natural Killer cells was performed to assess cytotoxicity. Apoptosis rate was quantified using flow cytometry. Results Perifosine treatment caused a reduction in leukemic infiltration in the spleens of BALB/c nude mice. In vitro , AKT inhibition reduced HL60 resistance to natural Killer-induced apoptosis. AKT inhibition suppressed the immune checkpoint proteins PD-L1, galectin-9, and CD122 in HL60 cells, but did not change the expression of their co-receptors PD1, Tim3, and CD96 on the natural Killer cell surface. In addition, the death receptors DR4, TNFR1, and FAS were overexpressed by AKT inhibition, thus increasing the susceptibility of HL60 cells to the extrinsic pathway of apoptosis. Conclusion The AKT pathway is involved in resistance to natural Killer-induced apoptosis in HL60 cells by regulating the expression of immune suppressor receptors. These findings highlight the importance of AKT in contributing to immune evasion mechanisms in acute myeloid leukemia and suggests the potential of AKT inhibition as an adjunct to immunotherapy.

Published
2023

4. Inhibition of Wnt/Beta-Catenin Pathway Impairs Natural Killer (NK) Cells Induced Apoptosis of Myeloid Leukemia Cells

Author

Datoguia, Tarcila Santos, primary, Varela, Vanessa Araújo, additional, Jardim, Elayne Bragança, additional, Amano, Mariane Tami, additional, Mazini, Natalia, additional, Bizarro, Bruna, additional, Almeida, Danilo Candido de, additional, Hamerschlak, Nelson, additional, Santos, Fabio Pires de Souza, additional, and Pereira, Welbert Oliveira, additional

Published
2020
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5. Physical exercise contributes to cisplatin-induced nephrotoxicity protection with decreased CD4+ T cells activation

Author

Miyagi, Mariana Yasue Saito, primary, Latancia, Marcela Teatin, additional, Testagrossa, Leonardo Abreu, additional, Andrade-Oliveira, Vinicius de, additional, Pereira, Welbert Oliveira, additional, Hiyane, Meire Ioshie, additional, Enjiu, Lucas Maceratesi, additional, Pisciottano, Marcus, additional, Seelaender, Marilia Cerqueira Leite, additional, Camara, Niels Olsen Saraiva, additional, and Amano, Mariane Tami, additional

Published
2018
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8. Presence of JAK2V617F Mutation in Endothelial Cells from Budd-Chiari Syndrome Patients Is Not Correlated with Ph-Negative Myeloproliferative Neoplasm

Author

Helman, Ricardo, primary, Pereira, Welbert Oliveira, additional, Campregher, Paulo Vidal, additional, Marti, Luciana Cavalheiro, additional, Hamerschlak, Nelson, additional, Bello, Isabel, additional, Nakashima, Sandra Saemi, additional, Chiattone, Carlos S, additional, and Santos, Fabio P S, additional

Published
2014
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9. Genomic Profile of Patients with Triple Negative (JAK2, CALR and MPL) Essential Thrombocythemia and Primary Myelofibrosis

Author

Campregher, Paulo Vidal, primary, Helman, Ricardo, additional, Pereira, Welbert Oliveira, additional, Puga, Renato D, additional, Lisboa, Bianca Garcia, additional, Ascendino, Evelyn Helena, additional, Miyagi, Mariana, additional, Yoshida, Sandra Nakashima, additional, Bello, Isabel, additional, Diniz, Michelli Silva, additional, Perini, Guilherme Fleury, additional, Velloso, Elvira, additional, Guerra, Joao Carlos, additional, Silveira, Paulo A.A., additional, Bacal, Nydia, additional, Petroni, Roberta Cardoso, additional, Musacchio, Juliane, additional, Santucci, Rodrigo, additional, Schriefer, Ana Fernandes, additional, Salvino, Marco A., additional, Rodolfo, Soares Almeida, additional, Tibúrcio, Manuela Pinto, additional, Perobelli, Leila Martins, additional, Souto, Eizabeth Xisto, additional, Farias, Danielle Leao Cordeiro De, additional, Higashi, Marcia, additional, Fadel, Adriana Valente, additional, Datoguia, Tarcila S, additional, Kassab, Carolina, additional, Padua, Leandro, additional, Bollman, Patricia W, additional, Costa, Fernando Ferreira, additional, Hamerschlak, Nelson, additional, and Santos, Fabio Pires de Souza, additional

Published
2014
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10. Whole Exome Sequencing of Philadelphia-Negative (Ph-negative) Myeloproliferative Neoplasms (MPNs) and Myelodysplastic/Myeloproliferative Disorders (MDS/MPD)

Author

Santos, Fabio P S, primary, Puga, Renato D, additional, Helman, Ricardo, additional, Pereira, Welbert Oliveira, additional, Datoguia, Tarcila S, additional, Lisboa, Bianca, additional, Miyagi, Mariana, additional, da Mata, Evelyn HA, additional, Santucci, Rodrigo, additional, Perini, Guilherme F, additional, Souto, Eizabeth Xisto, additional, Kassab, Carolina, additional, Padua, Leandro, additional, Bollman, Patricia W, additional, Soares, Rodolfo, additional, Higashi, Marcia, additional, Fadel, Adriana Valente, additional, Musacchio, Juliane, additional, Schriefer, Ana Fernandes, additional, Salvino, Marco Aurelio, additional, Farias, Danielle Leao Cordeiro De, additional, Perobelli, Leila Martins, additional, Silveira, Paulo A. A., additional, Bacal, Nydia, additional, Guerra, Joao Carlos, additional, Bezerra, Alanna Mara P. S, additional, Pasqualin, Denise da Cunha, additional, Lima, Marinus M, additional, Costa, Erika MM, additional, Mattos, Vinicius R. P., additional, Bello, Isabel C, additional, Diniz, Michelli Silva, additional, Nakashima, Sandra S, additional, Velloso, Elvira, additional, Hamerschlak, Nelson, additional, and Campregher, Paulo Vidal, additional

Published
2014
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11. The Presence of ASXL1 Mutations As Well As a Total Number of Myeloid Driver Mutations Higher Than Two Is Strongly Associated with the Diagnosis of Primary Myelofibrosis As Opposed to Essential Thrombocythemia

Author

Campregher, Paulo Vidal, primary, Helman, Ricardo, additional, Pereira, Welbert Oliveira, additional, Puga, Renato D, additional, Lisboa, Bianca, additional, Ascendino, Evelyn Helena, additional, Miyagi, Mariana, additional, Yoshida, Sandra Nakashima, additional, Bello, Isabel C, additional, Diniz, Michelli Silva, additional, Perini, Guilherme Fleury, additional, Datoguia, Tarcila S, additional, Velloso, Elvira, additional, Guerra, Joao Carlos, additional, Silveira, Paulo A. A., additional, Bacal, Nydia, additional, Petroni, Roberta Cardoso, additional, Musacchio, Juliane, additional, Santucci, Rodrigo, additional, Schriefer, Ana Fernandes, additional, Salvino, Marco A., additional, Soares, Rodolfo, additional, Tibúrcio, Manuela Pinto, additional, Perobelli, Leila Martins, additional, Souto, Eizabeth Xisto, additional, Farias, Danielle Leao Cordeiro De, additional, Higashi, Marcia, additional, Fadel, Adriana Valente, additional, Kassab, Carolina, additional, Padua, Leandro, additional, Bollman, Patricia W, additional, Pasqualin, Denise da Cunha, additional, Bezerra, Alanna Mara P. S, additional, Lima, Marinus de Moraes, additional, Costa, Erika MM, additional, Mattos, Vinicius R. P., additional, Costa, Fernando Ferreira, additional, Hamerschlak, Nelson, additional, and Santos, Fabio Pires de Souza, additional

Published
2014
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12. Incidence and Impact of Activating Mutations of the RAS-RAF-MEK-ERK Pathway in Patients with Philadelphia-Negative (Ph-negative) Myeloproliferative Neoplasms (MPNs) and Myelodysplastic/Myeloproliferative Disorders–Unclassified (MDS/MPD-U)

Author

Santos, Fabio PS, primary, Lisboa, Bianca, additional, Datoguia, Tarcila S, additional, Helman, Ricardo, additional, Pereira, Welbert Oliveira, additional, Puga, Renato D, additional, Miyagi, Mariana, additional, da Mata, Evelyn HA, additional, Santucci, Rodrigo, additional, Perini, Guilherme F, additional, Souto, Eizabeth Xisto, additional, Kassab, Carolina, additional, Padua, Leandro, additional, Bollman, Patricia W, additional, Higashi, Marcia, additional, Soares, Rodolfo, additional, Fadel, Adriana Valente, additional, Musacchio, Juliane, additional, Schriefer, Ana Fernandes, additional, Salvino, Marco Aurelio, additional, Farias, Danielle Leao Cordeiro De, additional, Perobelli, Leila Martins, additional, Costa, Erika MM, additional, Lima, Marinus M, additional, Mattos, Vinicius R. P., additional, Silveira, Paulo A. A., additional, Bacal, Nydia, additional, Guerra, Joao Carlos, additional, Bezerra, Alanna Mara P. S, additional, Pasqualin, Denise da Cunha, additional, Bello, Isabel C, additional, Diniz, Michelli Silva, additional, Nakashima, Sandra S, additional, Velloso, Elvira, additional, Hamerschlak, Nelson, additional, and Campregher, Paulo Vidal, additional

Published
2014
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13. MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a and microRNA-10b dysregulation implicated in cell proliferation

Author

Severino, Patricia, primary, Brüggemann, Holger, additional, Andreghetto, Flavia Maziero, additional, Camps, Carme, additional, Klingbeil, Maria de Fatima Garrido, additional, de Pereira, Welbert Oliveira, additional, Soares, Renata Machado, additional, Moyses, Raquel, additional, Wünsch-Filho, Victor, additional, Mathor, Monica Beatriz, additional, Nunes, Fabio Daumas, additional, Ragoussis, Jiannis, additional, and Tajara, Eloiza Helena, additional

Published
2013
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14. AKT inhibition interferes with the expression of immune checkpoint proteins and increases NK-induced killing of HL60-AML cells.

Author

Gama SM, Varela VA, Ribeiro NM, Bizzarro B, Hernandes C, Aloia TPA, Amano MT, and Pereira WO

Subjects
Animals, Mice, Humans, HL-60 Cells, Mice, Nude, Proto-Oncogene Proteins c-akt, Immune Checkpoint Proteins, Leukemia, Myeloid, Acute drug therapy
Abstract

Objective: To determine the role of the AKT pathway in the regulating of natural Killer-induced apoptosis of acute myeloid leukemia cells and to characterize the associated molecular mechanisms., Methods: BALB/c nude mice were injected with HL60 cells to induce a xenogenic model of subcutaneous leukemic tumors. Mice were treated with perifosine, and their spleens were analyzed using biometry, histopathology, and immunohistochemistry. Gene expression analysis in leukemia cells was performed by real-time PCR. Protein analysis of leukemia and natural Killer cells was performed by flow cytometry. AKT inhibition in HL60 cells, followed by co-culture with natural Killer cells was performed to assess cytotoxicity. Apoptosis rate was quantified using flow cytometry., Results: Perifosine treatment caused a reduction in leukemic infiltration in the spleens of BALB/c nude mice. In vitro , AKT inhibition reduced HL60 resistance to natural Killer-induced apoptosis. AKT inhibition suppressed the immune checkpoint proteins PD-L1, galectin-9, and CD122 in HL60 cells, but did not change the expression of their co-receptors PD1, Tim3, and CD96 on the natural Killer cell surface. In addition, the death receptors DR4, TNFR1, and FAS were overexpressed by AKT inhibition, thus increasing the susceptibility of HL60 cells to the extrinsic pathway of apoptosis., Conclusion: The AKT pathway is involved in resistance to natural Killer-induced apoptosis in HL60 cells by regulating the expression of immune suppressor receptors. These findings highlight the importance of AKT in contributing to immune evasion mechanisms in acute myeloid leukemia and suggests the potential of AKT inhibition as an adjunct to immunotherapy.

Published
2023
Full Text
View/download PDF

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