111 results on '"Perdigon, Gabriela"'
Search Results
2. Beneficial Effects of Probiotic Consumption on the Immune System
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Galdeano, Carolina Maldonado, Cazorla, Silvia Inés, Dumit, José María Lemme, Vélez, Eva, and Perdigón, Gabriela
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- 2019
3. Oral administration of milk fermented by Lactobacillus casei CRL431 was able to decrease metastasis from breast cancer in a murine model by modulating immune response locally in the lungs
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Mendez Utz, Virginia Emilce, Perdigón, Gabriela, and de Moreno de LeBlanc, Alejandra
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- 2019
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4. Lactobacillus casei CRL 431 administration decreases inflammatory cytokines in a diet-induced obese mouse model
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Novotny Núñez, Ivanna, Maldonado Galdeano, Carolina, de Moreno de LeBlanc, Alejandra, and Perdigón, Gabriela
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- 2015
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5. Evaluation of immune response, microbiota, and blood markers after probiotic bacteria administration in obese mice induced by a high-fat diet
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Novotny Núñez, Ivanna, Maldonado Galdeano, Carolina, de Moreno de LeBlanc, Alejandra, and Perdigón, Gabriela
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- 2014
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6. Thymus, undernutrition, and infection: Approaching cellular and molecular interactions
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Savino, Wilson, primary, Durães, Jonathan, additional, Maldonado-Galdeano, Carolina, additional, Perdigon, Gabriela, additional, Mendes-da-Cruz, Daniella Arêas, additional, and Cuervo, Patricia, additional
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- 2022
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7. The administration of milk fermented by the probiotic Lactobacillus casei CRL 431 exerts an immunomodulatory effect against a breast tumour in a mouse model
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Aragón, Félix, Carino, Silvia, Perdigón, Gabriela, and de Moreno de LeBlanc, Alejandra
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- 2014
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8. Influence of a probiotic lactobacillus strain on the intestinal ecosystem in a stress model mouse
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Palomar, Martin Manuel, Maldonado Galdeano, Carolina, and Perdigón, Gabriela
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- 2014
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9. Thymus, undernutrition, and infection: Approaching cellular and molecular interactions
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Savino, Wilson, Durães, Jonathan, Maldonado-Galdeano, Carolina, Perdigon, Gabriela, Mendes-da-Cruz, Daniella Arêas, Cuervo, Patricia, Savino, Wilson, Durães, Jonathan, Maldonado-Galdeano, Carolina, Perdigon, Gabriela, Mendes-da-Cruz, Daniella Arêas, and Cuervo, Patricia
- Abstract
Undernutrition remains a major issue in global health. Low protein-energy consumption, results in stunting, wasting and/or underweight, three deleterious forms of malnutrition that affect roughly 200 million children under the age of five years. Undernutrition compromises the immune system with the generation of various degrees of immunodeficiency, which in turn, renders undernourished individuals more sensitive to acute infections. The severity of various infectious diseases including visceral leishmaniasis (VL), influenza, and tuberculosis is associated with undernutrition. Immunosuppression resulting from protein-energy undernutrition severely impacts primary and secondary lymphoid organs involved in the response to related pathogens. The thymus—a primary lymphoid organ responsible for the generation of T lymphocytes—is particularly compromised by both undernutrition and infectious diseases. In this respect, we will discuss herein various intrathymic cellular and molecular interactions seen in undernutrition alone or in combination with acute infections. Many examples illustrated in studies on humans and experimental animals clearly revealed that protein-related undernutrition causes thymic atrophy, with cortical thymocyte depletion. Moreover, the non-lymphoid microenvironmental compartment of the organ undergoes important changes in thymic epithelial cells, including their secretory products such as hormones and extracellular matrix proteins. Of note, deficiencies in vitamins and trace elements also induce thymic atrophy. Interestingly, among the molecular interactions involved in the control of undernutrition-induced thymic atrophy is a hormonal imbalance with a rise in glucocorticoids and a decrease in leptin serum levels. Undernutrition also yields a negative impact of acute infections upon the thymus, frequently with the intrathymic detection of pathogens or their antigens. For instance, undernourished mice infected with Leishmania infantum (that causes VL)
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- 2022
10. Probiotics and the immune state
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Perdigón, Gabriela, Alvarez, Susana, and Fuller, Roy
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- 1992
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11. Survival effect of probiotics in a rat model of colorectal cancer treated with capecitabine
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Gigola, Graciela, primary, Carriere, Pedro, additional, Novoa Díaz, María Belén, additional, Perdigon, Gabriela, additional, Zwenger, Ariel Osvaldo, additional, and Gentili, Claudia, additional
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- 2021
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12. Gut immune stimulation by non pathogenic Gram(+) and Gram(−) bacteria. Comparison with a probiotic strain
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Dogi, C.A., Galdeano, C. Maldonado, and Perdigón, Gabriela
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- 2008
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13. Effect of long-term continuous consumption of fermented milk containing probiotic bacteria on mucosal immunity and the activity of peritoneal macrophages
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de Moreno de LeBlanc, A., Chaves, S., Carmuega, E., Weill, R., Antóine, J., and Perdigón, Gabriela
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- 2008
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14. Consumo de leches fermentadas probióticas y su impacto sobre el sistema inmune
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Maldonado Galdeano, María Carolina, Cazorla, Silvia Ines, Balcells, Maria Florencia, Martínez Monteros, María José, Lemme Dumit, José María, Perdigon, Gabriela del Valle, Ferrari, Alejandro, Vinderola, Celso Gabriel, and Weill, Ricardo
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purl.org/becyt/ford/1 [https] ,ALIMENTOS FERMENTADOS ,SKISTEMA INMUNE ,PROBIOTICOS ,purl.org/becyt/ford/1.6 [https] - Abstract
Los productos fermentados son conocidos desde épocas milenariaspor sus beneficios sobre la salud de los consumidores. A partir de allísurge el concepto de probióticos, que según la Organización Mundialde la Salud y el Código Alimentario Argentino son microorganismos vivosque, cuando se administran en cantidades adecuadas, ejercen unefecto benéfico sobre el consumidor. Muchos de estos microorganismoshan sido aislados de la microbiota del hospedador y son capacesde impactar positivamente en el sistema inmune de mucosa. En los últimostiempos, los estudios exhaustivos sobre estos microorganismoshan permitido conocer algunos de los mecanismos de acción por loscuales ejercen sus efectos. Algunas bacterias probióticas interaccionandirectamente con las células epiteliales intestinales, induciendo cambiosen las mismas, que llevan a la liberación de sustancias que permiteniniciar un diálogo entre las células y de ese modo orquestar la respuestainmune en intestino. La mayoría de los probióticos ingresan al organismoa través de los alimentos o suplementos alimenticios y una vez enel intestino, van a reforzar la barrera intestinal y mejorar el balance dela microbiota intestinal. Se ha demostrado de manera científica que elefecto no se limita al ambiente de intestino, sino que también puedetener efecto en otros sitios mucosos y no mucosos distantes. Estudiosrecientes demostraron que los probióticos pueden actuar a nivel de órganosdistantes, como timo.El timo es de fundamental importancia en la diferenciación y maduracióninmune de los linfocitos T, por lo que está íntimamente involucradoen el correcto funcionamiento del sistema inmune. Fisiológicamente,el timo involuciona con la edad, haciendo a estos individuos más vulnerablesa infecciones. Los cambios nutricionales y algunas infeccionestambién pueden afectar al timo. La posibilidad de prolongar el correctofuncionamiento de este órgano mediante el consumo de alimentos fermentadosque contengan microorganismos probióticos, resulta en unaestrategia atractiva y prometedora. En este capítulo mostramos parte dela evidencia científica que avala el consumo de probióticos y productosfermentados para mantener el balance del sistema inmune y mejorar lacalidad de vida. Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Balcells, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Martínez Monteros, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Lemme Dumit, José María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
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- 2020
15. Milk fermented by Lactobacillus helveticus R389 and its non-bacterial fraction confer enhanced protection against Salmonella enteritidis serovar Typhimurium infection in mice
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Vinderola, Gabriel, Matar, Chantal, and Perdigón, Gabriela
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- 2007
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16. Effects of the oral administration of the exopolysaccharide produced by Lactobacillus kefiranofaciens on the gut mucosal immunity
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Vinderola, Gabriel, Perdigón, Gabriela, Duarte, Jairo, Farnworth, Edward, and Matar, Chantal
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- 2006
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17. Immunomodulating capacity of commercial fish protein hydrolysate for diet supplementation
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Duarte, Jairo, Vinderola, Gabriel, Ritz, Barry, Perdigón, Gabriela, and Matar, Chantal
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- 2006
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18. Effects of kefir fractions on innate immunity
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Vinderola, Gabriel, Perdigon, Gabriela, Duarte, Jairo, Thangavel, Deepa, Farnworth, Edward, and Matar, Chantal
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- 2006
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19. Effects of milk fermented by Lactobacillus helveticus R389 on immune cells associated to mammary glands in normal and a breast cancer model
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de Moreno de LeBlanc, Alejandra, Matar, Chantal, Thériault, Catherine, and Perdigón, Gabriela
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- 2005
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20. Microorganismos probióticos y sus efectos benéficos en la salud humana. Realidad vs. mito
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Perdigon, Gabriela del Valle, Maldonado Galdeano, María Carolina, and Cazorla, Silvia Ines
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Medicina Básica ,CIENCIAS MÉDICAS Y DE LA SALUD ,INTESTINAL MICROBIOTA ,MICROBIOTA INTESTINAL ,Inmunología ,purl.org/becyt/ford/3 [https] ,purl.org/becyt/ford/3.1 [https] ,SALUD ,HEALTH ,PROBIÓTICOS ,PROBIOTICS - Abstract
El intestino humano es el hábitat natural de una población numerosa, diversa y dinámica de microorganismos, denominada microbiota intestinal. Esta comunidad diversa de microorganismos tiene funciones específicas, que son claves en el mantenimiento de una buena salud. Uno de los aspectosrelevantes de la misma es la capacidad para promover el establecimiento y la maduración del sistema inmune. A partir del conocimiento de las funciones de la microbiota, surge la asociación de efectos nocivos y beneficiosos de bacterias vivas con la salud y, casi paralelamente a ello, el concepto de probióticos. Estos últimos puedenformar parte de la microbiota intestinal o también se encuentran disponibles en numerosos alimentos como yogur, conservas, vinos y pan, que ha llevado a numerosos científicos a analizar el efecto probiótico tanto en el huésped sano como en grupos de riesgo. Al presente existe una vasta evidencia científica experimental que demuestra el efecto benéfico de los probióticos, sugiriendo la potencialidad de su empleo en humanos; sin embargo, es necesario continuar con la validación clínica en diferentes patologías concretas que aseguren lainocuidad de los mismos. The human intestine is the natural habitat of a large, diverse and dynamic population of microorganisms, called intestinal microbiota. This diverse community of microorganisms has specific functions, which are keys in the maintenance of good health. One of the relevant aspects of it, is the ability to promote the establishment and maturation of the immune system.From the knowledge of the functions of the microbiota, there arises the association of harmful and beneficial effects of living bacteria with health and almost parallel to it, the concept of probiotics. The latter can be part of the gut microbiota or are also available in many foods such as yogurt, preserves, wines, bread, which has led many scientists to analyze the probiotic effect in both healthy hosts and risk groups. At present there is vast experimental scientific evidence that provesthe beneficial effect of probioticssuggesting the potential of its use in humans. However, it is necessary to continue with clinical validation in different specific pathologies, which ensure the safety of them. Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Cazorla, Silvia Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
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- 2018
21. Should yoghurt cultures be considered probiotic?
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Guarner, Francisco, Perdigon, Gabriela, Corthier, Gérard, Salminen, Seppo, Koletzko, Berthold, and Morelli, Lorenzo
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- 2005
22. Evidencias científicas de los mecanismos inducidos por probióticos en la modulación del sistema inmune
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Maldonado Galdeano, María Carolina, Cazorla, Silvia Ines, Perdigon, Gabriela del Valle, Lemme Dumit, José María, De Paula, Juan Andrés, Vinderola, Celso Gabriel, and Weill, Ricardo
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purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,purl.org/becyt/ford/3.3 [https] ,Nutrición, Dietética ,CIENCIAS MÉDICAS Y DE LA SALUD ,Biología ,Ciencias de la Salud ,purl.org/becyt/ford/3 [https] ,PROBIOTICOS ,SISTEMA INMUNE ,purl.org/becyt/ford/1.6 [https] ,MICROBIOTA ,CIENCIAS NATURALES Y EXACTAS - Abstract
El tracto gastrointestinal es uno de los ecosistemas microbiológicamente más activos, pues contiene una gran masa de bacterias, levaduras y otros microorganismos. Esta comunidad diversa y dinámica tiene funciones específicas, que son claves en el mantenimiento de una buena salud. El papel primordial de esta microbiota, es mantener la homeostasis de la mucosa intestinal y promover la maduración del sistema inmune.Algunas de las bacterias que constituyen la microbiota y otras que forman parte de los alimentos (yogur, leches cultivadas, quesos, y muchos otros alimentos no lácteos), tienen la capacidad de actuar de manera beneficiosa sobre la salud. Estos microorganismos reciben elnombre de probióticos y ha llevado a numerosos científicos a analizar el efecto de éstos tanto en huésped sano como en grupos de riesgo.Al presente existe una vasta evidencia científica experimental que demuestra el efecto benéfico de probióticos, y se describen los posibles mecanismos que intervienen en dichos efectos. Todos estos estudios dan sustento científico para el empleo de probióticos en humano. Si bien resulta crucial complementar estas evidencias con la experimentación clínica dirigida a demostrar mecanismos, beneficios y seguridad en el humano, todo parece indicar que el consumo de alimentos adicionados con probióticos podrían ser altamente beneficiosos en el hombre. Sin embargo, es necesario continuar con la validación clínica en diferentes patologías concretas, que aseguren la inocuidad de los mismos. Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Lemme Dumit, José María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
- Published
- 2018
23. Malnutrition: Role of the Diet on the Microbiota and In the Functioning of the Gut Immune System
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Maldonado Galdeano, María Carolina, Novotny Núñez, Ivanna, and Perdigon, Gabriela del Valle
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Ciencias Biológicas ,purl.org/becyt/ford/1 [https] ,Otras Ciencias Biológicas ,OBESITY ,GUT MICROBIOTA ,purl.org/becyt/ford/1.6 [https] ,PROBIOTICS ,CIENCIAS NATURALES Y EXACTAS ,MUCOSAL IMMUNE SYSTEM - Abstract
The intestinal microbiota in health is characterized by stability and diversity; any changes under different metabolic or inflammatory diseases cause an imbalance, changes in the composition and consequently modification in the intestinal homeostasis.The intestinal microbiota is influenced by the microorganisms that enter with the diet. Malnutrition processes, both obesity and malnourishment, induces changes in the intestinal microbiota composition, affecting mainly lactobacillus, bifidobacteria and bacteroides populations. These changes are accompanied by alterations in the intestinal villi architecture and in the intestinal barrier function.The intestinal ecosystem is a complex microenvironment where, multiple cells type (prokaryote and eukaryote) interacting constantly. The microbiota modifications induced by changes in the eating habits have repercussion on intestinal immunity.Probiotic microorganisms contained in different foods constitute an alternative to induce a positive balance in the microbiota. On the other hand several studies demonstrate their ability to stimulate the immune system at the intestinal level.We summarize here the results published specifically on the relationship between microbiota, obesity and intestinal immune system. Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
- Published
- 2016
24. Protective effect of lactobacillus casei CRL 431 against salmonella in a mouse model: mechanisms involved
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Castillo, Natalia Alejandra, de Moreno, Maria Alejandra, Maldonado Galdeano, María Carolina, Perdigon, Gabriela del Valle, and Roma, Anastasia
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Otras Ciencias de la Salud ,CIENCIAS MÉDICAS Y DE LA SALUD ,MUCOSAL IMMUNITY ,food and beverages ,Ciencias de la Salud ,SALMONELLA ,MECHANISMS - Abstract
Salmonella species are Gram-negative bacilli that constitute the causing agents of several animal and human illnesses. They cause acute or chronic infections that can be local (e.g gastroenteritis produced by non-typhoidal serovars) or systemic (e.g. enteric fever produced by typhoidal serovars). Non-typhoidal serovars produce 93.8 million cases of gastroenteritis worldwide, leading to 155,000 deaths each year. According to a foodborne disease surveillance network data supported by the World Health Organization (2001-2005), Salmonella (S.) Enteritidis was the most common serotype worldwide (65% of the isolates), followed by S. Typhimurium (12%) and S. Newport (4%). Numerous studies have proposed the use of probiotics to improve gut health, especially in the protection against enteropathogens. In this sense, the probiotic strain Lactobacillus (L.). casei CRL 431 showed protective effect against S. Typhimurium in BALB/c mouse model. This chapter reports an update of the main biological and immune mechanisms involved in the protection observed with this probiotic bacterium. The effect of other two lactobacilli strains (L. delbruekii subsp. bulgaricus CRL 423 and L. acidophilus CRL 730), which displayed immunostimulating ability but did not show protective capacity against Salmonella was also added comparing with L. casei CRL431. This comparative analysis was conducted to define some effects on the mucosal immune system that would be desired in a probiotic strain to achieve protection against this particular pathogen. The results showed that L. casei CRL 431 was the only strain with protective effect against Salmonella. The continuous treatment (before and after infection) with this strain improved animal survival, and diminished pathogen counts in liver, spleen and large intestine. L. casei CRL 431 administration increased also the number of IgA(+) cells in lamina propria of the small intestine, as well as total and specific anti-Salmonella s-IgA in intestinal fluids. Increased IgA levels correlated with the increased IL-6 levels observed in the animals that received this probiotic bacterium. The preventive administration of L. casei before the infection stimulated the phagocytic activity of peritoneal, Peyer’s patches and spleen macrophages. Probiotic administration post infection increased expression and secretion of IFNγ. The probiotic bacterium also attenuated the intestinal inflammation by reducing tissue damage and polymorphonuclear infiltration, and maintaining the levels of the regulatory cytokine IL-10. Although L. acidophilus CRL 730 increased the number of IgA(+) cells, and L. bulgaricus CRL 423 increased phagocytic activity of macrophages, these mechanisms by themself were not enough to confer protection against S. Typhimurium infection in mouse. Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
- Published
- 2014
25. Difference in the signals induced by commensal or probiotic bacteria to the gut epithelial and immune cells
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Maldonado Galdeano, María Carolina, Dogi, Cecilia Ana, Perdigon, Gabriela del Valle, Kitazawa, Haruki, Villena, Julio Cesar, and Alvarez, Gladis Susana
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Ciencias Biológicas ,Otras Ciencias Biológicas ,COMMENSAL BACTERIA ,IMMUNE SYSTEM ,MECHANIMS ,PROBIOTICS ,CIENCIAS NATURALES Y EXACTAS - Abstract
The main function of the immune system is to protect the body against harmful agents or pathogens, therefore, the immune cells are distributed throughout the body mainly at the mucosal sites, places that are continuously exposed to antigens from the external environment. However, one of the unresolved mysteries is how immune cells can distinguish between self and non-self antigens, especially between pathogenic and non-pathogenic microorganisms. Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Dogi, Cecilia Ana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
- Published
- 2014
26. Probiotics as protective agents against Enterobacteria
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Perdigon, Gabriela del Valle, Castillo, Natalia Alejandra, Maldonado Galdeano, María Carolina, and de Moreno, Maria Alejandra
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Medicina Básica ,CIENCIAS MÉDICAS Y DE LA SALUD ,MUCOSAL IMMUNITY ,Inmunología ,ENTEROPATHOGEN ,purl.org/becyt/ford/3 [https] ,purl.org/becyt/ford/3.1 [https] ,SALMONELLA ,PROBIOTICS - Abstract
Salmonella is one of the major causative agents of foodborne diseases, morbidity and mortality worldwide. Salmonella enterica serovar typhimurium(S. typhimurium) produces in mice an infection with similar pathogenesis and clinical manifestations than S. typhiin humans. Probiotics influence the innate and adaptive immunity and the activity of the intestinal microbiota. This knowledge allowed us to propose the use of probiotic bacteria against Salmonella infection. On this topic, the mechanisms mediating this protection are not yet established. We determine the effect of the oral administration of probiotic bacterium L. casei CRL-431 on the biological and immune mechanisms involved in the prevention and treatment of the S. Typhimurium infection, using BALB/c mice. Oral administration of probiotic to healthy mice, induced activation of gut immune cells in a regulated state. The continuous L. casei CRL-431 administration (previous and post-infection) protected mice challenged with S. Typhimurium. The mechanisms involved were: a) The modulation of innate immune response by increased TLR expression on epithelial and immune cells. b) Epithelial cell activation, evidenced by increases in secretion of IL-6 and MCP-1. c) Increased number of macrophages, dendritic cells and IgA (+) cells in lamina propria of the small intestine, and increased secretion of total S-IgA. d)Reduction of inflammation, with reduced TNF-alpha levels and myeloperoxidase activity, less PMN infiltration in lamina propria and regulation by IL-10. e)Increased phagocytic activity in macrophage isolated from Peyer’s patches, spleen and peritoneum, correlated with an increase of IFNgamma. The results showed that L. casei CRL-431 induces biological and immune mechanisms, which confer resistance to S. typhimuriuminfection, showing lesser severity of the infection. The safety of continuous administration of this probiotc strain, allows us to suggest its use as an adjuvant of the mucosal immune system in the prevention, and during Salmonella infection. Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina; Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina; Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina
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- 2013
27. Bacterias probióticas como suplemento dietario promisorio para la salud
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Novotny Núñez, Ivanna, Maldonado Galdeano, María Carolina, Castillo, Natalia Alejandra, de Moreno, Maria Alejandra, and Perdigon, Gabriela del Valle
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Medicina Básica ,leche fermentada probiotica ,CIENCIAS MÉDICAS Y DE LA SALUD ,malnutricion ,Inmunología ,purl.org/becyt/ford/3 [https] ,purl.org/becyt/ford/3.1 [https] ,probioticos ,sistema inmune ,infeccion intestinal - Abstract
El ecosistema intestinal constituye un microambiente complejo donde las células inmunes e intestinales están en constante interacción con la microbiota normal. La maduración y la modulación del sistema inmune intestinal se encuentran influenciadas por el establecimiento de la microbiota comensal, cuyo equilibrio puede ser afectado (benéficamente o no) por microorganismos que ingresan a través de la alimentación. Entre estos microorganismos se encuentran los probióticos que se caracterizan por conferir un efecto beneficioso en la salud del hospedador. Entre las propiedades atribuidas a estos microorganismos, podemos mencionar la capacidad de reforzar la barrera intestinal y la de mejorar la respuesta inmune. Utilizando modelos experimentales en ratón, se demostró que los microorganismos probióticos y/o las leches fermentadas que los contienen pueden modular el sistema inmune sistémico y de mucosa, proporcionando protección contra bacterias enteropatógenas y actuando como adyuvantes de la respuesta inmune sistémica frente a una vacunación en hospedadores inmunodeprimidos por malnutrición. The intestinal ecosystem is a complex microenvironment where immune and intestinal cells are in constant interaction with the normal microbiota. The maturation and modulation of the intestinal immune system are influenced by the establishment of commensal microbiota, whose balance can be affected by microorganisms that enter through the food. Some of these microorganisms called probiotics, confer a health benefit on the host. Among the properties attributed to these microorganisms, it could be mentioned the ability to reinforce the intestinal barrier and enhance the immune response. Using experimental models performed in mice, it was demonstrated that the probiotic microorganisms and / or fermented milks containing them, could modulate the systemic and mucosal immune system, providing protection against enteropathogenic bacteria and acting as adjuvant of the systemic immune response against a vaccination in immunosuppressed hosts by malnutrition. Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
- Published
- 2012
28. Bacterias gram (+) probióticas: influencia sobre el sistema inmune
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Maldonado Galdeano, María Carolina and Perdigon, Gabriela del Valle
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purl.org/becyt/ford/3.3 [https] ,Salud Ocupacional ,CIENCIAS MÉDICAS Y DE LA SALUD ,MICROBIOTA INTESTINAL ,Ciencias de la Salud ,purl.org/becyt/ford/3 [https] ,PROBIOTICOS ,IMMUNE SYSTEM ,PROBIOTICS ,SISTEMA INMUNE INTESTINAL - Abstract
La microbiota intestinal juega un papel importante en el desarrollo y maduración del sistema inmune de mucosas. Entre la gran población de bacterias que forman parte de la misma, se encuentran las bacterias acido lácticas y las bifidobacterias, las cuales son usadas frecuentemente como probióticos debido a los beneficios sobre la salud atribuidos a las mismas. Para mantener la homeostasis intestinal es imprescindible el balance de esta microbiota, el cual puede ser afectado por una gran cantidad de factores como tratamiento con antibióticos, stress, quiomoterapia y por la dieta entre otros. De allí que surge la posibilidad de emplear a los microorganismos probióticos presentes en muchos alimentos para mejorar el balance intestinal y actuar como adyuvantes del sistema inmune. Conocer el mecanismo por el cual estas bacterias pueden tener efecto sobre el sistema inmune es de importancia para poder utilizarlos de modo correcto. Los estudios realizados muestran que dichos microorganismos ayudan a reforzar las defensas naturales a nivel de mucosas para mantener en estado de alerta permanente al sistema de defensa, de manera que la respuesta frente a un estímulo sea rápida y controlada. The intestinal microbiota plays an important role in the development and maturation of the mucosal immune system. Lactic acid bacteria are constituents of the microbiota and they are frequently used as probiotics due of the health benefits attributed to them. To maintain intestinal homeostasis is essential the balance of this microbiota. This balance can be affected by many factors such as antibiotics, stress, diet quiomoterapia and others. Hence arises the possibility of using the probiotic microorganisms present in many foods to improve intestinal balance and act as immune adjuvants. The Knowledge the mechanism by which these bacteria may have an effect on the immune system is important to use them correctly. Studies show that these organisms help reinforce the natural defenses at mucosal to maintain constant alertness to the defense system, so that the response to a stimulus is rapid and controlled manner. Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Perdigon, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
- Published
- 2011
29. Leches fermentadas, sus aplicaciones en cáncer e inflamación intestinal
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de Moreno, Maria Alejandra, Perdigon, Gabriela del Valle, and Marcos Sánchez, Ascensión
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CIENCIAS MÉDICAS Y DE LA SALUD ,INFLAMACION INTESTINAL ,LECHES FERMENTADAS ,PROBIOTICOS ,CANCER ,Otras Ciencias Médicas - Abstract
Las bacterias ácido-lácticas (BAL) están presentes en muchos alimentos, como el yogur, y son frecuentemente utilizadas como probióticos para favorecer algunas funciones biológicas del hospedador como anfitrión. Un gran número de investigadores han evaluado los efectos terapéuticos del yogur y las BAL contra enfermedades como el cáncer, infecciones e inflamación intestinal. Se ha descrito la prevención del cáncer con el consumo de las BAL y el yogur en modelos experimentales. El efecto preventivo de los probióticos empleado sobre la carcinogénesis podría estar asociado con cambios en la microbiota intestinal capaces de suprimir el crecimiento de las bacterias que convierten las sustancias procarcinogénicas en carcinogénicas; de este modo, se reduce la cantidad de carcinógenos en el intestino. Otros mecanismos podrían estar relacionados con la inflamación mediada por mecanismos inmunológicos. Nuestro grupo ha trabajado en dos tipos de cáncer en los que la administración de leches fermentadas con BAL ha conseguido un efecto beneficioso en el cáncer de colon quimicamente inducido y en el cáncer de mama en modelo experimental endocrino-dependiente. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina
- Published
- 2011
30. Role of Probiotics and Functional Foods in Health: Gut Immune Stimulation by Two Probiotic Strains and a Potential Probiotic Yoghurt
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Galdeano, Carolina Maldonado, primary, Nunez, Ivanna Novotny, additional, Carmuega, Esteban, additional, Moreno de LeBlanc, Alejandra de, additional, and Perdigon, Gabriela, additional
- Published
- 2015
- Full Text
- View/download PDF
31. Probiotics in cancer prevention
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de Moreno, Maria Alejandra, Bibas Bonet, María Eugenia, Leblanc, Jean Guy Joseph, Sesma, Fernando Juan Manuel, Perdigon, Gabriela del Valle, Ross Watson, Ronald, and Preedy, Victor
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BREAST TUMOUR ,Medicina Básica ,CIENCIAS MÉDICAS Y DE LA SALUD ,LACTIC ACID BACTERIA ,Inmunología ,COLON CANCER ,YOGURT - Abstract
Probiotics have been given credit for numerous health-promoting effects; one of which is their anticarcinogenic properties. Even when the epidemiological data and those obtained from human trails are promising, animal models are still necessary to elucidate the mechanisms by which they can act. The effects of probiotics on intestinal disorders have been the most extensively studied. In a model of colon cancer inhibition through yoghurt feeding, yoghurt exerts its antitumor activity through different mechanisms: 1) modulates the immune system response and exerts an anti-inflammatory effect in the large intestine; 2) activates apoptosis pathways; and 3) decreases certain enzyme activities involved in the development of tumors in the intestine. The introduction of antioxidant enzyme genes in probiotic strains that have natural anti-inflammatory properties could generate very potent strains that could be used for the prevention of inflammatory diseases or post-cancer drug treatments. It is also possible to obtain a beneficial effect in other mucosal site distant to the intestine with the oral administration of a probiotics or fermented milks. Fermented milk administration can regulate the immune response in an estrogen- dependent breast tumor model. Probiotic strain selection plays an important role in the antitumoral effects observed and the principal cause that can be attributed to the probiotic and / or fermented product against cancer is the immune surveillance, which differs according to the site where the tumor attacks. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Bibas Bonet, María Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología. Cátedra de Inmunología; Argentina
- Published
- 2010
32. Alimentos Funcionales: su relación con la Inmunonutrición
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Perdigon, Gabriela del Valle, de Moreno, Maria Alejandra, Maldonado Galdeano, María Carolina, Chaves, Analia Silvina, Carmuega, Esteban, and Weill, Ricardo
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Medicina Básica ,CIENCIAS MÉDICAS Y DE LA SALUD ,Inmunología ,ALIMENTOS FUNCIONALES ,PROBIOTICOS ,purl.org/becyt/ford/3 [https] ,purl.org/becyt/ford/3.1 [https] ,INMUNONUTRICION - Abstract
En base al concepto de que un estilo de vida y dieta saludables contribuyen al bienestar general de las personas, es que surgen los llamados alimentos funcionales. Estos son considerados como alimentos o componentes de la dieta, que se consumen como parte de una dieta normal y que más allá de la nutrición básica aportan componentes biológicamente activos, que ofrecen beneficios para la salud y reducen el riesgo de sufrir enfermedades. Puede tratarse de un alimento natural, un alimento al que se ha añadido, eliminado o modificado un componente por medios biotecnológicos, un alimento en el que se ha modificado la biodisponibilidad de uno o más de sus componentes o una combinación de las anteriores. Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Maldonado Galdeano, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Chaves, Analia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Carmuega, Esteban. Nutritia; Argentina Fil: Weill, Ricardo. Danone; Argentina
- Published
- 2009
33. Antitumour Activity Of Yogurt
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de Moreno, Maria Alejandra and Perdigon, Gabriela del Valle
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YOGHURT ,Medicina Básica ,CIENCIAS MÉDICAS Y DE LA SALUD ,Inmunología ,food and beverages ,COLON CANCER ,ANTITUMOUR RESPONSE - Abstract
Several studies have demonstrated that fermented milk consumption decrease the incidence of colorectal cancer. Using a chemically induced murine colon cancer model it was reported that conventional yogurt inhibits tumour development. In this model, the inflammatory immune response caused by the carcinogen (DMH) showed a great increase in IgG+ B cells, CD8+ T lymphocytes and in proinflammatory cytokines (TNFá and IFNã). Yogurt feeding inhibited tumour development by decreasing the inflammatory immune response and increasing the number of IgA+ cells, CD4+ T lymphocytes, cytokines such as IL-10 and decreasing NO radicals. Yogurt also induced the apoptosis mechanisms. The local immune stimulation produced by yogurt feeding increased monocytes/macrophages population and the cytokines release in the nodular tissue and in the Peyer’s patches suggesting that these cells could be responsible for IFNγ and TNFα production. The enhancement of IL-10 found would favour the regulation of the immune response, not only in the inhibition model of the tumour growth, but also when yogurt is given long term. The immune mechanisms involved by yogurt to decrease the inflammatory immune response caused by the carcinogen were different to those observed with an antiinflammatory drug (indomethacin). Indomethacin did not increase immune infiltrative cell activity in the large intestine and the cytokine levels were diminished. Nitric oxide synthase enzyme determinations showed that in mice fed with yogurt, the IFNγ enhancement was not related to inflammation, but to an immunomodulation. We demonstrated that the only single yogurt supplementation was unable to inhibit tumour development in the initiation stage, however it inhibited the tumour growth (promption and progression) when it was administered cyclically after tumour induction. Cellular apoptosis increase observed could explain the importance for the TNFα levels found in the mice fed long term with yogurt. The normal microflora has an important function in the intestinal inflammatory process preceding tumour development, lactic acid bacteria present in yogurt play a role in this process since it has been shown that these bacteria and fermented milk products act on the microbial enzyme activities associated with colon carcinogenesis. This chapter will show that yogurt can inhibit the promotion and progression of chemically induced colon cancer in mice through its antiinflammatory effect, cell apoptosis and by its immunomodulating properties Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
- Published
- 2006
34. Remote-site stimulation and duration of the immune response by kefir
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Vinderola, Celso Gabriel, Duarte, J., Thangavel, D., Perdigon, Gabriela del Valle, Farnworth, E., and Matar, C.
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Alimentos y Bebidas ,purl.org/becyt/ford/2 [https] ,Otras Ingenierías y Tecnologías ,Remote-site ,INGENIERÍAS Y TECNOLOGÍAS ,kefir ,immune response ,purl.org/becyt/ford/2.11 [https] - Abstract
Kefir is a fermented milk (drink) produced by the action of lactic acid bacteria, yeasts and acetic acid bacteria. We recently reported a comparative study on the effect of kefir containing viable or non-viable bacteria by studying their modulatory activity on the intestinal immune response. A functional dose was established in a murine model and the pattern of regulatory and pro-inflammatory cytokines induced was also studied. The existence of a common mucosal immune system implies that the immune cells stimulated in one mucosal tissue can spread and relocate through various mucosal sites. The aim of this work was to determine the effect of an oral administration of kefir on the duration of the intestinal mucosa immune response and the modulatory activity in distal mucosal sites, specifically in the peritoneal and pulmonary acrophages and in the bronchial tissue. BALB/c mice were fed with kefir or pasteurized kefir at doses previously determined as functional for intestinal mucosa immunomodulation. Kefir feeding was stopped and the number of IgA, IgG, IL-4, IL-6, IL-10, IIFNg and TNFa producing cells was determined in the lamina propria of small intestine immediately, and after 2 and 7 days of kefir withdrawal. IgAproducing cells were also measured in the bronchial tissue of lungs immediately and 2 and 7 days after kefir withdrawal. Phagocytic activity of peritoneal and pulmonary macrophages was also determined. The oral administration of kefir or pasteurized kefir increased the number of IgA+ cells not only in the gut lamina propria, but also in the bronchial tissue, supporting the concept of local antibody secretion after remote-site stimulation in the intestinal tract. Both peritoneal and pulmonary macrophages were activated by kefir or pasteurized kefir feeding. Peritoneal macrophages were stimulated faster than pulmonary macrophages (for kefir). The enhanced phagocytic activity achieved by kefir or pasteurized kefir lasted longer for the peritoneal than for the pulmonary macrophages. Due to the increased bronchial IgA and phagocytic activity of pulmonary macrophages after kefir feeding observed in this study, the oral administration of kefir could act as a natural adjuvant for enhancing the specific immune response against respiratory pathogens. The parameters studied returned to control values within a week of cessation of kefir administration. This would suggest that there is a low risk of overstimulating the gut mucosal immune system during periodic consumption of viable kefir. Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Duarte, J.. University of Moncton; Canadá Fil: Thangavel, D.. University of Moncton; Canadá Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina Fil: Farnworth, E.. 4Agriculture and Agri-Food Canada; Canadá Fil: Matar, C.. University of Moncton; Canadá
- Published
- 2005
35. Reduction of β-Glucuronidase and nitroreductase activity by yoghurt in a murine colon cancer model
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de Moreno, Maria Alejandra and Perdigon, Gabriela del Valle
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YOGHURT ,Medicina Básica ,CIENCIAS MÉDICAS Y DE LA SALUD ,STRATER BACTERIA ,Inmunología ,food and beverages ,purl.org/becyt/ford/3 [https] ,purl.org/becyt/ford/3.1 [https] ,COLON CANCER ,B-GLUCURONIDASE ,NITROREDUCTASE - Abstract
Yoghurt feeding inhibits induced colon cancer in mice. Several studies showed the immunomodulatory effect of yoghurt which can explain this inhibition. It is possible that yoghurt bacteria can also affect gut flora enzymes related to colon carcinogenesis as reported for other probiotics in different animal tumours. The aim of this work was to evaluate the role of yoghurt starter bacteria and their cell-free fermentation products on the reduction of procarcinogen enzyme activities (β-glucuronidase and nitroreductase). Mice injected with 1,2-dimethylhydrazine (DMH) and fed with yoghurt were used for this study. Mice given milk or yoghurt supernatant (cell free extract) were used to evaluate if the yoghurt antitumour effect is due to the starter bacteria or other components released during fermentation, that could inhibit these enzymes. We determined that yoghurt by itself maintained enzymes activities similar or lower than nontreatment control group, and the enzyme activity was also lower than milk or yoghurt supernatant groups. DMH increased the activity of the enzymes. Mice injected with DMH and fed cyclically with yoghurt presented lower enzymes activities than the tumour control group. Feeding yoghurt decreased procarcinogenic enzyme levels in the large intestine contents of mice bearing colon tumour. The results of this study provide another mechanism by which yoghurt starter bacteria interact with the large intestine of the mice and prevent colon cancer. Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
- Published
- 2005
36. Gut mucosal immunostimulation by lactic acid bacteria
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Vintiñi, Elisa Ofelia, Alvarez, Gladis Susana, Medina, Marcela Susana, Medici, Marta Graciela, Valverde de Budeguer, Marta Hercilia, and Perdigon, Gabriela del Valle
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Medicina Básica ,CIENCIAS MÉDICAS Y DE LA SALUD ,MUCOSAL IMMNUNE CELLS ,LACTIC ACID BACTERIA ,INTESTINE ,Inmunología - Abstract
The beneficial properties of lactic acid bacteria (LAB) on human health have been frequently demonstrated. The interaction of LAB with the lymphoid cells associated to the gut to activate the mucosal immune system and the mechanisms by which they can exert an adjuvant effect is still unclear, as well as if this property is common for all the LAB. We studied the influence of the oral administration of different geneous of LAB such as Lactobacillus casei, L. acidophilus, L. rhamnosus, L. delbrueckii subsp. bulgaricus, L. plantarum, Lactococcus lactis and Streptococcus thermophilus. We determined if the LAB assayed were able to stimulate the specific, the non-specific immune response (inflammatory response), or both. We demonstrated that all the bacteria assayed were able to increase the number of IgA producing cells associated to the lamina propria of small intestine. This effect was dose dependent. The increase in IgA+ producing cells was not always correlated with an increase in the CD4+ T cell number, indicating that some LAB assayed only induced clonal expansion of B cells triggered to produce IgA. Most of them, induced an increase in the number of cells involved in the inflammatory immune response. CD8+ T cell were diminished or not affected, with exception of L. plantarum that induced an increase at low dose. This fact would mean that LAB are unable to induce cytotoxicity mechanisms. We demonstrated the importance in the selection of LAB to be used as gut mucosal adjuvant. The different behaviours observed among them on the gut mucosal immune response, specially those that induce inflammatory immune response, show that not all the LAB can be used as oral adjuvant and that the beneficial effect of them can not generalized to genous or specie. The immunoadjuvant capacity would be a property of the strain assayed. Fil: Vintiñi, Elisa Ofelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina Fil: Alvarez, Gladis Susana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Medina, Marcela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Medici, Marta Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Valverde de Budeguer, Marta Hercilia. Universidad Nacional de Tucuman. Facultad de Medicina. Departamento Biomedico. Cátedra de Histologia; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
- Published
- 2000
37. ILSI Brazil International Workshop on Functional Foods: a narrative review of the scientific evidence in the area of carbohydrates, microbiome, and health
- Author
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Latulippe, MarieE., primary, Meheust, Agnès, additional, Augustin, Livia, additional, Benton, David, additional, Berčík, Přemysl, additional, Birkett, Anne, additional, Eldridge, AlisonL., additional, Faintuch, Joel, additional, Hoffmann, Christian, additional, Miller Jones, Julie, additional, Kendall, Cyril, additional, Lajolo, Franco, additional, Perdigon, Gabriela, additional, Antonio Prieto, Pedro, additional, Rastall, RobertA., additional, Sievenpiper, JohnL., additional, Slavin, Joanne, additional, and Wenzel de Menezes, Elizabete, additional
- Published
- 2013
- Full Text
- View/download PDF
38. Yoghurt Consumption Regulates the Immune Cells Implicated in Acute Intestinal Inflammation and Prevents the Recurrence of the Inflammatory Process in a Mouse Model
- Author
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Chaves, Silvina, primary, Perdigon, Gabriela, additional, and De Leblanc, Alejandra De Moreno, additional
- Published
- 2011
- Full Text
- View/download PDF
39. Use of superoxide dismutase and catalase producing lactic acid bacteria in TNBS induced Crohn's disease in mice
- Author
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LeBlanc, Jean Guy, primary, del Carmen, Silvina, additional, Miyoshi, Anderson, additional, Azevedo, Vasco, additional, Sesma, Fernando, additional, Langella, Philippe, additional, Bermúdez-Humarán, Luis G., additional, Watterlot, Laurie, additional, Perdigon, Gabriela, additional, and de Moreno de LeBlanc, Alejandra, additional
- Published
- 2011
- Full Text
- View/download PDF
40. Evaluation of the Anti-Inflammatory Effect of Milk Fermented by a Strain of IL-10-Producing Lactococcus lactis Using a Murine Model of Crohn’s Disease
- Author
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del Carmen, Silvina, primary, de Moreno de LeBlanc, Alejandra, additional, Perdigon, Gabriela, additional, Bastos Pereira, Vanessa, additional, Miyoshi, Anderson, additional, Azevedo, Vasco, additional, and LeBlanc, Jean Guy, additional
- Published
- 2011
- Full Text
- View/download PDF
41. Anti-infective mechanisms induced by a probiotic Lactobacillus strain against Salmonella enterica serovar Typhimurium infection
- Author
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de Moreno de LeBlanc, Alejandra, primary, Castillo, Natalia A., additional, and Perdigon, Gabriela, additional
- Published
- 2010
- Full Text
- View/download PDF
42. Anti-inflammatory Properties of Lactic Acid Bacteria: Current Knowledge,Applications and Prospects
- Author
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LeBlanc, Jean, primary, de LeBlanc, Alejandra, additional, Perdigon, Gabriela, additional, Miyoshi, Anderson, additional, Rochat, Tatiana, additional, Bermudez-Humaran, Luis, additional, Langella, Philippe, additional, Sesma, Fernando, additional, and Azevedo, Vasco, additional
- Published
- 2008
- Full Text
- View/download PDF
43. Effect of milk fermented with aLactobacillus helveticusR389(+) proteolytic strain on the immune system and on the growth of 4T1 breast cancer cells in mice
- Author
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Rachid, Mirta, primary, Matar, Chantal, additional, Duarte, Jairo, additional, and Perdigon, Gabriela, additional
- Published
- 2006
- Full Text
- View/download PDF
44. Role of Intestinal Epithelial Cells in Immune Effects Mediated by Gram-Positive Probiotic Bacteria: Involvement of Toll-Like Receptors
- Author
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Vinderola, Gabriel, primary, Matar, Chantal, additional, and Perdigon, Gabriela, additional
- Published
- 2005
- Full Text
- View/download PDF
45. Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model
- Author
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GAUFFIN CANO, PAOLA, primary, AG蹺RO, GRACIELA, additional, and PERDIGON, GABRIELA, additional
- Published
- 2002
- Full Text
- View/download PDF
46. Effect of Lactobacillus casei and Yogurt Administration on Prevention of Pseudomonas aeruginosa Infection in Young Mice
- Author
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Alvarez, Susana, primary, Herrero, Claudia, additional, Bru, Elena, additional, and Perdigon, Gabriela, additional
- Published
- 2001
- Full Text
- View/download PDF
47. Immunomodulating effects of milks fermented by Lactobacillus helveticus and its non-proteolytic variant
- Author
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MATAR, CHANTAL, primary, VALDEZ, JUAN CARLOS, additional, MEDINA, MARCELA, additional, RACHID, MIRTHA, additional, and PERDIGON, GABRIELA, additional
- Published
- 2001
- Full Text
- View/download PDF
48. Lactic Acid Bacteria Induce Apoptosis Inhibition in Salmonella typhimurium Infected Macrophages
- Author
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Carlos Valdez, Juan, primary, Rachid, Mirta, additional, Gobbato, Nadia, additional, and Perdigon, Gabriela, additional
- Published
- 2001
- Full Text
- View/download PDF
49. Optimal Effect of Lactobacillus delbruecki subsp. bulgaricus, Among Other Lactobacilli Species, on the Number of IgA and Mast Cells Associated with the Mucosa in Immunosuppressed Mice
- Author
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Bibas Bonet, Maria E., primary, De Petrino, Silvia F., additional, Meson, Oscar, additional, De Budeguer, Marta V., additional, and Perdigon, Gabriela, additional
- Published
- 1999
- Full Text
- View/download PDF
50. Immunostimulating activity of cell walls from lactic acid bacteria and related species
- Author
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De Ambrosini, Vilma Morata, primary, Gonzalez, Silvia, additional, Perdigon, Gabriela, additional, De Ruiz Holgado, Aida P., additional, and Oliver, Guillermo, additional
- Published
- 1998
- Full Text
- View/download PDF
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