1. Comparative effects of ammonium nitrogen on perchlorate degradation performance under heterotrophic condition with different carbon sources.
- Author
-
Xie Y, Gu L, Wang Y, Liu W, and Huo Y
- Subjects
- Heterotrophic Processes, Bacteria metabolism, Bacteria drug effects, Nitrogen metabolism, Ammonium Compounds metabolism, Quaternary Ammonium Compounds metabolism, Glucose metabolism, Perchlorates metabolism, Carbon chemistry, Carbon metabolism, Water Pollutants, Chemical metabolism, Biodegradation, Environmental
- Abstract
Perchlorate (ClO
4 - ) mainly exists in the form of ammonium perchlorate in industrial production. However, the degradation mechanisms of different concentrations of ammonium nitrogen (NH4 + -N) and ClO4 - mixed pollutants in the environment are not well understood. This study aims to explore the potential of different types of carbon sources for ClO4 - and NH4 + -N biodegradation. Experimental results showed that the concentration and type of carbon sources are decisive to simultaneous removal of NH4 + -N and ClO4 - . Under condition of C(COD)/C(ClO4 - ) ratio of 21.15 ± 4.40, the simultaneously removal efficiency of ClO4 - and NH4 + -N in acetate (Ace) was relatively higher than that in methanol (Met). C(NH4 + -N)/C(ClO4 - ) ratio of 9.66 ± 0.51 and C(COD)/C(ClO4 - ) ratio of 2.51 ± 0.87 promoted ClO4 - reduction in glucose-C (Glu-C). However, high concentration of Glu could cause pH decrease (from 7.57 to 4.59), thereby inhibiting ClO4 - reduction. High-throughput sequencing results indicated that Proteobacteria and Bacteroidetes have made a major contribution to the simultaneous removal of NH4 + -N and ClO4 - . They are two representative bacterial phyla for participating in both ClO4 - reduction and denitrification. Notably, the abundance of main ClO4 - degrading bacteria (such as Proteobacteria, Chloroflexi, and Firmicutes) significantly increased by 528.57 % in Glu-C. It can be inferred that the concentration of carbon source and NH4 + -N were the most important factors determining the removal efficiency of ClO4 - by influencing changes in the core microbial community. This study will provide new techniques and mechanistic insights for the simultaneous removal of mixed ClO4 - and nitrogen pollutants, which can also provide theoretical support for innovation in future biological treatment processes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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