1. Association of hemolytic activity of Pseudomonas entomophila, a versatile soil bacterium, with cyclic lipopeptide production
- Author
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Isabelle Vallet-Gely, Bruno Lemaitre, Luis A. Augusto, Pierre Cosson, Maria Péchy-Tarr, Gérard Bolbach, Alexey Novikov, Peter Liehl, Martine Caroff, Christoph Keel, Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Centre de génétique moléculaire (CGM), Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Département de Microbiologie Fondamentale, Université de Lausanne (UNIL), Département de Physiologie Cellulaire et Métabolisme, Université de Genève (UNIGE), Université de Lausanne = University of Lausanne (UNIL), and Université de Genève = University of Geneva (UNIGE)
- Subjects
RNA, Untranslated ,MESH: Drosophila ,Swarming motility ,MESH: Amino Acid Sequence ,MESH: Virulence ,MESH: Genome, Bacterial ,Applied Microbiology and Biotechnology ,MESH: RNA, Untranslated ,chemistry.chemical_compound ,MESH: Lipopeptides ,Peptides, Cyclic/*biosynthesis/chemistry/metabolism ,MESH: Endopeptidase Clp ,RNA, Untranslated/genetics/metabolism ,MESH: Pest Control, Biological ,MESH: Animals ,MESH: Endopeptidases ,MESH: Bacterial Proteins ,Soil Microbiology ,Repressor Proteins/genetics/metabolism ,chemistry.chemical_classification ,0303 health sciences ,MESH: Gene Expression Regulation, Bacterial ,Ecology ,biology ,Virulence ,Hemolytic Agents ,Pseudomonas ,Lipopeptide ,Endopeptidase Clp ,Pseudomonas putida ,RNA, Bacterial ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Biochemistry ,MESH: Repressor Proteins ,Pseudomonadales ,Bacterial Proteins/genetics/metabolism ,Drosophila ,MESH: Genes, Bacterial ,MESH: RNA, Bacterial ,Pseudomonas entomophila ,RNA, Bacterial/genetics/metabolism ,Biotechnology ,Virulence Factors ,Peptides, Cyclic ,Lipopeptides/*biosynthesis/chemistry/metabolism ,Virulence Factors/genetics/metabolism ,Microbiology ,Virulence/genetics ,03 medical and health sciences ,Lipopeptides ,Bacterial Proteins ,Nonribosomal peptide ,Endopeptidases ,Invertebrate Microbiology ,Animals ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Amino Acid Sequence ,ddc:612 ,Pest Control, Biological ,Drosophila/genetics/metabolism ,MESH: Peptides, Cyclic ,Endopeptidases/genetics/metabolism ,030304 developmental biology ,MESH: Virulence Factors ,030306 microbiology ,MESH: Pseudomonas ,Gene Expression Regulation, Bacterial ,Hemolytic Agents/chemistry/*metabolism ,biology.organism_classification ,Repressor Proteins ,chemistry ,MESH: Soil Microbiology ,Genes, Bacterial ,Pseudomonas/*genetics/metabolism/pathogenicity ,Endopeptidase Clp/genetics/metabolism ,MESH: Hemolytic Agents ,Genome, Bacterial ,Food Science - Abstract
Pseudomonas entomophila is an entomopathogenic bacterium that is able to infect and kill Drosophila melanogaster upon ingestion. Its genome sequence suggests that it is a versatile soil bacterium closely related to Pseudomonas putida . The GacS/GacA two-component system plays a key role in P. entomophila pathogenicity, controlling many putative virulence factors and AprA, a secreted protease important to escape the fly immune response. P. entomophila secretes a strong diffusible hemolytic activity. Here, we showed that this activity is linked to the production of a new cyclic lipopeptide containing 14 amino acids and a 3-C 10 OH fatty acid that we called entolysin. Three nonribosomal peptide synthetases (EtlA, EtlB, EtlC) were identified as responsible for entolysin biosynthesis. Two additional components (EtlR, MacAB) are necessary for its production and secretion. The P. entomophila GacS/GacA two-component system regulates entolysin production, and we demonstrated that its functioning requires two small RNAs and two RsmA-like proteins. Finally, entolysin is required for swarming motility, as described for other lipopeptides, but it does not participate in the virulence of P. entomophila for Drosophila . While investigating the physiological role of entolysin, we also uncovered new phenotypes associated with P. entomophila , including strong biocontrol abilities.
- Published
- 2010
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