1. Isolation and characterisation of GLP-1 7-36 amide from rat intestine Elevated levels in diabetic rats
- Author
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B. Kreymann, M. A. Ghatei, S.M. Kanse, S.R. Bloom, Yiangos Yiangou, and Graham R. Williams
- Subjects
Glucagon-like peptide-1 ,endocrine system ,medicine.medical_specialty ,Peptide termination factor ,Arginine ,Colon ,Glucagon-Like Peptides ,Biophysics ,(Rat) ,Mass spectrometry ,Biochemistry ,High-performance liquid chromatography ,Mass Spectrometry ,Diabetes Mellitus, Experimental ,Eating ,Residue (chemistry) ,Diabetes mellitus ,Glucagon-Like Peptide 1 ,Ileum ,Structural Biology ,Internal medicine ,Genetics ,medicine ,Animals ,Tissue Distribution ,Intestinal Mucosa ,Molecular Biology ,Chromatography, High Pressure Liquid ,Chromatography ,Gastrointestinal tract ,Molecular mass ,Chemistry ,digestive, oral, and skin physiology ,Rats, Inbred Strains ,Cell Biology ,Chromatography, Ion Exchange ,Glucagon ,medicine.disease ,Peptide Fragments ,Rats ,Intestines ,Molecular Weight ,Endocrinology ,Peptides ,hormones, hormone substitutes, and hormone antagonists - Abstract
Glucagon-like peptide-1 (GLP-1) was purified to homogeneity by HPLC and anion-exchange chromatography. A molecular mass of 3297.4 Da was obtained by FAB mass spectrometry which corresponded exactly to GLP-1 7–36 NH2, providing evidence that amidation occurs at an arginine residue during the post-translational processing of GLP-1. The distribution of GLP-1 7–36 NH2-like immunoreactivity (GLP-1 7–36 NH2 IR) was determined in the rat gastrointestinal tract. Highest concentrations were found in terminal ileum and colon. Streptozocin-induced diabetic rats, who showed a significant increase in food intake, had a significant increase of GLP-1 7–36 NH2 IR in the colon.
- Published
- 1988