1. Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365.
- Author
-
Liu D, Garrigues S, Culleton H, McKie VA, and de Vries RP
- Subjects
- Promoter Regions, Genetic, Recombinant Proteins metabolism, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Proteolysis, Peptide Hydrolases metabolism, Peptide Hydrolases genetics, Transcription Factors metabolism, Transcription Factors genetics, Trans-Activators, Aspergillus genetics, Aspergillus metabolism, Aspergillus enzymology, Fungal Proteins genetics, Fungal Proteins metabolism
- Abstract
Aspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is primarily attributed to the lack of functionality of its gene's promoter sequence. In contrast, a different mechanism is likely causing the lack of PrtT activity, which is the main transcriptional regulator of protease production. The findings presented here not only expand our understanding of the genetic basis behind the distinct characteristics of A. vadensis CBS 113365, but also underscore its potential as a favourable alternative for recombinant protein production., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF