26 results on '"Pensado-López, Alba"'
Search Results
2. Edelfosine nanoemulsions inhibit tumor growth of triple negative breast cancer in zebrafish xenograft model
- Author
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Saraiva, Sofia M., Gutiérrez-Lovera, Carlha, Martínez-Val, Jeannette, Lores, Sainza, Bouzo, Belén L., Díez-Villares, Sandra, Alijas, Sandra, Pensado-López, Alba, Vázquez-Ríos, Abi Judit, Sánchez, Laura, and de la Fuente, María
- Published
- 2021
- Full Text
- View/download PDF
3. An in vitro model for osteoarthritis using long‐cultured inflammatory human macrophages repeatedly stimulated with TLR agonists
- Author
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Ummarino, Aldo, primary, Pensado‐López, Alba, additional, Migliore, Roberta, additional, Alcaide‐Ruggiero, Lourdes, additional, Calà, Nicholas, additional, Caputo, Michele, additional, Gambaro, Francesco M., additional, Anfray, Clément, additional, Ronzoni, Flavio L., additional, Kon, Elizaveta, additional, Allavena, Paola, additional, and Andón, Fernando Torres, additional
- Published
- 2023
- Full Text
- View/download PDF
4. A novel in vitro model of long-lasting inflammation using primary human macrophages for osteoarthritis research
- Author
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Ummarino, Aldo, primary, Pensado-López, Alba, additional, Migliore, Roberta, additional, Alcaide-Ruggiero, Lourdes, additional, Calà, Nicholas, additional, Caputo, Michele, additional, Gambaro, Francesco Manlio, additional, Anfray, Clément, additional, Ronzoni, Flavio L., additional, Kon, Elizaveta, additional, Allavena, Paola, additional, and Andón, Fernando Torres, additional
- Published
- 2023
- Full Text
- View/download PDF
5. Zebrafish as a platform to evaluate the potential of lipidic nanoemulsions for gene therapy in cancer
- Author
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Cascallar, María, primary, Hurtado, Pablo, additional, Lores, Saínza, additional, Pensado-López, Alba, additional, Quelle-Regaldie, Ana, additional, Sánchez, Laura, additional, Piñeiro, Roberto, additional, and de la Fuente, María, additional
- Published
- 2022
- Full Text
- View/download PDF
6. What zebrafish and nanotechnology can offer for cancer treatments in the age of personalized medicine
- Author
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Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cascallar, María, Alijas, Sandra, Pensado López, Alba, Vázquez Ríos, Abi Judit, Sánchez Piñón, Laura Elena, Piñeiro, Roberto, De la Fuente, María, Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cascallar, María, Alijas, Sandra, Pensado López, Alba, Vázquez Ríos, Abi Judit, Sánchez Piñón, Laura Elena, Piñeiro, Roberto, and De la Fuente, María
- Abstract
Cancer causes millions of deaths each year and thus urgently requires the development of new therapeutic strategies. Nanotechnology-based anticancer therapies are a promising approach, with several formulations already approved and in clinical use. The evaluation of these therapies requires efficient in vivo models to study their behavior and interaction with cancer cells, and to optimize their properties to ensure maximum efficacy and safety. In this way, zebrafish is an important candidate due to its high homology with the human genoma, its large offspring, and the ease in developing specific cancer models. The role of zebrafish as a model for anticancer therapy studies has been highly evidenced, allowing researchers not only to perform drug screenings but also to evaluate novel therapies such as immunotherapies and nanotherapies. Beyond that, zebrafish can be used as an “avatar” model for performing patient-derived xenografts for personalized medicine. These characteristics place zebrafish in an attractive position as a role model for evaluating novel therapies for cancer treatment, such as nanomedicine.
- Published
- 2022
7. Zebrafish as a platform to evaluate the potential of lipidic nanoemulsions for gene therapy in cancer
- Author
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Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cascallar, María, Lores, Saínza, Pensado López, Alba, Quelle Regaldie, Ana, Sánchez Piñón, Laura, Piñeiro, Roberto, Hurtado, Pablo, Fuente Freire, María de la, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cascallar, María, Lores, Saínza, Pensado López, Alba, Quelle Regaldie, Ana, Sánchez Piñón, Laura, Piñeiro, Roberto, Hurtado, Pablo, and Fuente Freire, María de la
- Abstract
Gene therapy is a promising therapeutic approach that has experienced significant groth in recent decades, with gene nanomedicines reaching the clinics. However, it is still necessary to continue developing novel vectors able to carry, protect, and release the nucleic acids into the target cells, to respond to the widespread demand for new gene therapies to address current unmet clinical needs. We propose here the use of zebrafish embryos as an in vivo platform to evaluate the potential of newly developed nanosystems for gene therapy applications in cancer treatment. Zebrafish embryos have several advantages such as low maintenance costs, transparency, robustness, and a high homology with the human genome. In this work, a new type of putrescine-sphingomyelin nanosystems (PSN), specifically designed for cancer gene therapy applications, was successfully characterized and demonstrated its potential for delivery of plasmid DNA (pDNA) and miRNA (miR). On one hand, we were able to validate a regulatory effect of the PSN/miR on gene expression after injection in embryos of 0 hpf. Additionally, experiments proved the potential of the model to study the transport of the associated nucleic acids (pDNA and miR) upon incubation in zebrafish water. The biodistribution of PSN/pDNA and PSN/miR in vivo was also assessed after microinjection into the zebrafish vasculature, demonstrating that the nucleic acids remained associated with the PSN in an in vivo environment, and could successfully reach disseminated cancer cells in zebrafish xenografts. Altogether, these results demonstrate the potential of zebrafish as an in vivo model to evaluate nanotechnology-based gene therapies for cancer treatment, as well as the capacity of the developed versatile PSN formulation for gene therapy applications
- Published
- 2022
8. Desarrollo y caracterización de una línea mutante de pez cebra para auts2a, gen candidato a trastornos del espectro autista
- Author
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Sánchez Piñón, Laura Elena, Allegue Toscano, Catarina, Universidade de Santiago de Compostela. Escola de Doutoramento Internacional (EDIUS), Universidade de Santiago de Compostela. Programa de Doutoramento en Medicina Molecular, Pensado López, Alba, Sánchez Piñón, Laura Elena, Allegue Toscano, Catarina, Universidade de Santiago de Compostela. Escola de Doutoramento Internacional (EDIUS), Universidade de Santiago de Compostela. Programa de Doutoramento en Medicina Molecular, and Pensado López, Alba
- Abstract
La etiología multifactorial de los trastornos del espectro autista (TEA) dificulta en gran medida la comprensión de los mecanismos desencadenantes, por lo que investigar la implicación de genes candidatos es fundamental. El empleo de líneas mutantes de pez cebra ofrece información con gran potencial para ser trasladada a la práctica clínica. En esta tesis se ha desarrollado una línea mutante para auts2a, ortólogo del gen humano AUTS2, mediante CRISPR/Cas9, y se ha caracterizado a nivel fenotípico, celular y transcriptómico. Conocidas las alteraciones que la disrupción del gen provoca, se pretende establecer a auts2a como potencial diana terapéutica para la búsqueda de nuevos tratamientos y así implementar el consejo genético, lo que redundaría en una mejora de la calidad de vida de los pacientes y sus familiares.
- Published
- 2022
9. What Zebrafish and Nanotechnology Can Offer for Cancer Treatments in the Age of Personalized Medicine
- Author
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Cascallar, María, primary, Alijas, Sandra, additional, Pensado-López, Alba, additional, Vázquez-Ríos, Abi, additional, Sánchez, Laura, additional, Piñeiro, Roberto, additional, and de la Fuente, María, additional
- Published
- 2022
- Full Text
- View/download PDF
10. Desarrollo y caracterización de una línea mutante de pez cebra para auts2a, gen candidato a trastornos del espectro autista
- Author
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Pensado López, Alba, Sánchez Piñón, Laura Elena, Allegue Toscano, Catarina, Universidade de Santiago de Compostela. Escola de Doutoramento Internacional (EDIUS), and Universidade de Santiago de Compostela. Programa de Doutoramento en Medicina Molecular
- Subjects
TEA ,Investigación::24 Ciencias de la vida::2409 Genética::240999 Otras (Genética molecular) [Materias] ,Neurodesarrollo ,Pez cebra ,AUTS2 ,CRISPR/Cas9 - Abstract
La etiología multifactorial de los trastornos del espectro autista (TEA) dificulta en gran medida la comprensión de los mecanismos desencadenantes, por lo que investigar la implicación de genes candidatos es fundamental. El empleo de líneas mutantes de pez cebra ofrece información con gran potencial para ser trasladada a la práctica clínica. En esta tesis se ha desarrollado una línea mutante para auts2a, ortólogo del gen humano AUTS2, mediante CRISPR/Cas9, y se ha caracterizado a nivel fenotípico, celular y transcriptómico. Conocidas las alteraciones que la disrupción del gen provoca, se pretende establecer a auts2a como potencial diana terapéutica para la búsqueda de nuevos tratamientos y así implementar el consejo genético, lo que redundaría en una mejora de la calidad de vida de los pacientes y sus familiares.
- Published
- 2022
11. Zebrafish Models for the Safety and Therapeutic Testing of Nanoparticles with a Focus on Macrophages
- Author
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Pensado-López, Alba, primary, Fernández-Rey, Juan, additional, Reimunde, Pedro, additional, Crecente-Campo, José, additional, Sánchez, Laura, additional, and Torres Andón, Fernando, additional
- Published
- 2021
- Full Text
- View/download PDF
12. Zebrafish Models for the Safety and Therapeutic Testing of Nanoparticles with a Focus on Macrophages
- Author
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Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Pensado López, Alba, Fernández Rey, Juan Manuel, Reimunde, Pedro, Crecente Campo, José, Sánchez Piñón, Laura, Torres Andón, Fernando, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Pensado López, Alba, Fernández Rey, Juan Manuel, Reimunde, Pedro, Crecente Campo, José, Sánchez Piñón, Laura, and Torres Andón, Fernando
- Abstract
New nanoparticles and biomaterials are increasingly being used in biomedical research for drug delivery, diagnostic applications, or vaccines, and they are also present in numerous commercial products, in the environment and workplaces. Thus, the evaluation of the safety and possible therapeutic application of these nanomaterials has become of foremost importance for the proper progress of nanotechnology. Due to economical and ethical issues, in vitro and in vivo methods are encouraged for the testing of new compounds and/or nanoparticles, however in vivo models are still needed. In this scenario, zebrafish (Danio rerio) has demonstrated potential for toxicological and pharmacological screenings. Zebrafish presents an innate immune system, from early developmental stages, with conserved macrophage phenotypes and functions with respect to humans. This fact, combined with the transparency of zebrafish, the availability of models with fluorescently labelled macrophages, as well as a broad variety of disease models offers great possibilities for the testing of new nanoparticles. Thus, with a particular focus on macrophage–nanoparticle interaction in vivo, here, we review the studies using zebrafish for toxicological and biodistribution testing of nanoparticles, and also the possibilities for their preclinical evaluation in various diseases, including cancer and autoimmune, neuroinflammatory, and infectious diseases.
- Published
- 2021
13. Edelfosine nanoemulsions inhibit tumor growth of triple negative breast cancer in zebrafish xenograft model
- Author
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Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Gutiérrez Lovera, Carlha, Martinez-Val, Jeannette, Lores Touriño, Sainza, LÓPEZ BOUZO, BELÉN, Díez Villares, Sandra, Sandra, Alijas Pérez, Pensado López, Alba, Vázquez Ríos, Abi Judit, Sánchez Piñón, Laura, de la Fuente Freire, Maria, Saraiva, Sofía M., Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Gutiérrez Lovera, Carlha, Martinez-Val, Jeannette, Lores Touriño, Sainza, LÓPEZ BOUZO, BELÉN, Díez Villares, Sandra, Sandra, Alijas Pérez, Pensado López, Alba, Vázquez Ríos, Abi Judit, Sánchez Piñón, Laura, de la Fuente Freire, Maria, and Saraiva, Sofía M.
- Abstract
Triple negative breast cancer (TNBC) is known for being very aggressive, heterogeneous and highly metastatic. The standard of care treatment is still chemotherapy, with adjacent toxicity and low efficacy, highlighting the need for alternative and more effective therapeutic strategies. Edelfosine, an alkyl-lysophospholipid, has proved to be a promising therapy for several cancer types, upon delivery in lipid nanoparticles. Therefore, the objective of this work was to explore the potential of edelfosine for the treatment of TNBC. Edelfosine nanoemulsions (ET-NEs) composed by edelfosine, Miglyol 812 and phosphatidylcholine as excipients, due to their good safety profile, presented an average size of about 120 nm and a neutral zeta potential, and were stable in biorelevant media. The ability of ET-NEs to interrupt tumor growth in TNBC was demonstrated both in vitro, using a highly aggressive and invasive TNBC cell line, and in vivo, using zebrafish embryos. Importantly, ET-NEs were able to penetrate through the skin barrier of MDA-MB 231 xenografted zebrafish embryos, into the yolk sac, leading to an effective decrease of highly aggressive and invasive tumoral cells’ proliferation. Altogether the results demonstrate the potential of ET-NEs for the development of new therapeutic approaches for TNBC
- Published
- 2021
14. Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells
- Author
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Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cabezas Sáinz, Pablo, Coppel, Carlos, Pensado López, Alba, Fernández García, Pedro, Muinelo Romay, Laura, López López, Rafael, Rubiolo Gaytán, Juan Andrés, Sánchez Piñón, Laura Elena, Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cabezas Sáinz, Pablo, Coppel, Carlos, Pensado López, Alba, Fernández García, Pedro, Muinelo Romay, Laura, López López, Rafael, Rubiolo Gaytán, Juan Andrés, and Sánchez Piñón, Laura Elena
- Abstract
Published studies show that most of the human cancer xenograft studies in zebrafish embryos have used incubation temperatures in the range of 32–34 °C for 3–6 days post-injection, trying to find a compromise temperature between the zebrafish embryos (28 °C) and the human injected cells (37 °C). While this experimental setup is widely used, a question remains: is possible to overcome the drawbacks caused by a suboptimal temperature for the injected cells? To clarify the effect of temperature and injected cells on the host, in this study, we analyzed the development and health of the last in response to different temperatures in the presence or absence of injected human cancer cells. Comparing different incubation temperatures (28, 34 and 36 °C), we determined morphological abnormalities and developmental effects in injected and non-injected embryos at different time points. Besides this, the expression of selected genes was determined by qPCR to determine temperature affected metabolic processes in the embryos. The results indicate that an incubation temperature of 36 °C during a period of 48 h is suitable for xenotransplantation without morphological or metabolic changes that could be affecting the host or the injected cells, allowing them to proliferate near their optimal temperature
- Published
- 2021
15. Métodos experimentales en el pez cebra (Danio rerio) para la caracterización de genes humanos relacionados con trastornos del neurodesarrollo
- Author
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Martínez Portela, Paulino, Pensado López, Alba, Universidade de Santiago de Compostela. Facultade de Veterinaria, Fernández Santos, Maider, Martínez Portela, Paulino, Pensado López, Alba, Universidade de Santiago de Compostela. Facultade de Veterinaria, and Fernández Santos, Maider
- Abstract
Los trastornos del neurodesarrollo son un grupo de trastornos que se manifiestan en períodos tempranos del desarrollo. Entre ellos se encuentran la discapacidad intelectual y los trastornos del espectro autista, que se solapan con gran frecuencia y en cuya etiología influyen factores genéticos y ambientales. Los análisis realizados para descubrir genes involucrados en la discapacidad intelectual han señalado la implicación de TRIP12, el cual también puede provocar autismo. Para investigar la implicación de TRIP12 en este tipo de trastornos, se realizó un bloqueo transitorio del gen mediante morfolinos en pez cebra, analizando el fenotipo morfológico resultante y la locomoción, y se realizó una validación de genes housekeeping así como del bloqueo transitorio a través de análisis de expresión génica por qPCR. Asimismo, se caracterizaron genotípica y fenotípicamente individuos de una línea mutante en pez cebra de trip12 generada mediante el sistema CRISPR/Cas9. Los resultados del análisis fenotípico de los individuos con bloqueo transitorio del gen revelan diferencias significativas (p < 0,05) en la longitud del cuerpo, el diámetro de la cabeza, la distancia del otolito al ojo y el área del ojo, siendo todas las medidas menores en individuos wild type. En relación al análisis fenotípico de los individuos mutantes, existe un aumento estadísticamente significativo respecto a los individuos wild type en todas las medidas (p < 0,05), a excepción del diámetro de la cabeza, que no presenta diferencias en ningún grupo. En cuanto al análisis de locomoción, se detectaron diferencias significativas (p < 0,05), presentando los individuos morfantes un menor movimiento que los wild type tanto en condiciones de luz como de oscuridad.
- Published
- 2021
16. Cellular and Molecular Mechanisms Underlying Glioblastoma and Zebrafish Models for the Discovery of New Treatments
- Author
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Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Reimunde Figueira, Pedro Marcos, Pensado López, Alba, Carreira Crende, Martín, Lombao Iglesias, Vanesa, Sánchez Piñón, Laura Elena, Torrecilla Parra, Marta, Ramírez, Cristina M., Anfray, Clément, Torres Andón, Fernando, Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Reimunde Figueira, Pedro Marcos, Pensado López, Alba, Carreira Crende, Martín, Lombao Iglesias, Vanesa, Sánchez Piñón, Laura Elena, Torrecilla Parra, Marta, Ramírez, Cristina M., Anfray, Clément, and Torres Andón, Fernando
- Abstract
Glioblastoma (GBM) is the most common of all brain malignant tumors; it displays a median survival of 14.6 months with current complete standard treatment. High heterogeneity, aggressive and invasive behavior, the impossibility of completing tumor resection, limitations for drug administration and therapeutic resistance to current treatments are the main problems presented by this pathology. In recent years, our knowledge of GBM physiopathology has advanced significantly, generating relevant information on the cellular heterogeneity of GBM tumors, including cancer and immune cells such as macrophages/microglia, genetic, epigenetic and metabolic alterations, comprising changes in miRNA expression. In this scenario, the zebrafish has arisen as a promising animal model to progress further due to its unique characteristics, such as transparency, ease of genetic manipulation, ethical and economic advantages and also conservation of the major brain regions and blood–brain–barrier (BBB) which are similar to a human structure. A few papers described in this review, using genetic and xenotransplantation zebrafish models have been used to study GBM as well as to test the anti-tumoral efficacy of new drugs, their ability to interact with target cells, modulate the tumor microenvironment, cross the BBB and/or their toxicity. Prospective studies following these lines of research may lead to a better diagnosis, prognosis and treatment of patients with GBM
- Published
- 2021
17. Zebrafish Models for the Safety and Therapeutic Testing of Nanoparticles with a Focus on Macrophages
- Author
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Pensado-López, Alba, Fernández-Rey, Juan, Reimunde, Pedro, Crecente-Campo, José, Sánchez, Laura, Torres Andón, Fernando, Pensado-López, Alba, Fernández-Rey, Juan, Reimunde, Pedro, Crecente-Campo, José, Sánchez, Laura, and Torres Andón, Fernando
- Abstract
[Abstract] New nanoparticles and biomaterials are increasingly being used in biomedical research for drug delivery, diagnostic applications, or vaccines, and they are also present in numerous commercial products, in the environment and workplaces. Thus, the evaluation of the safety and possible therapeutic application of these nanomaterials has become of foremost importance for the proper progress of nanotechnology. Due to economical and ethical issues, in vitro and in vivo methods are encouraged for the testing of new compounds and/or nanoparticles, however in vivo models are still needed. In this scenario, zebrafish (Danio rerio) has demonstrated potential for toxicological and pharmacological screenings. Zebrafish presents an innate immune system, from early developmental stages, with conserved macrophage phenotypes and functions with respect to humans. This fact, combined with the transparency of zebrafish, the availability of models with fluorescently labelled macrophages, as well as a broad variety of disease models offers great possibilities for the testing of new nanoparticles. Thus, with a particular focus on macrophage–nanoparticle interaction in vivo, here, we review the studies using zebrafish for toxicological and biodistribution testing of nanoparticles, and also the possibilities for their preclinical evaluation in various diseases, including cancer and autoimmune, neuroinflammatory, and infectious diseases.
- Published
- 2021
18. Cellular and Molecular Mechanisms Underlying Glioblastoma and Zebrafish Models for the Discovery of New Treatments
- Author
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Reimunde, Pedro, Pensado-López, Alba, Carreira Crende, Martín, Lombao Iglesias, Vanesa, Sánchez Piñón, Laura, Torrecilla-Parra, Marta, Ramírez Hidalgo, Cristina, Anfray, Clément, Torres Andón, Fernando, Reimunde, Pedro, Pensado-López, Alba, Carreira Crende, Martín, Lombao Iglesias, Vanesa, Sánchez Piñón, Laura, Torrecilla-Parra, Marta, Ramírez Hidalgo, Cristina, Anfray, Clément, and Torres Andón, Fernando
- Abstract
[Abstract] Glioblastoma (GBM) is the most common of all brain malignant tumors; it displays a median survival of 14.6 months with current complete standard treatment. High heterogeneity, aggressive and invasive behavior, the impossibility of completing tumor resection, limitations for drug administration and therapeutic resistance to current treatments are the main problems presented by this pathology. In recent years, our knowledge of GBM physiopathology has advanced significantly, generating relevant information on the cellular heterogeneity of GBM tumors, including cancer and immune cells such as macrophages/microglia, genetic, epigenetic and metabolic alterations, comprising changes in miRNA expression. In this scenario, the zebrafish has arisen as a promising animal model to progress further due to its unique characteristics, such as transparency, ease of genetic manipulation, ethical and economic advantages and also conservation of the major brain regions and blood–brain–barrier (BBB) which are similar to a human structure. A few papers described in this review, using genetic and xenotransplantation zebrafish models have been used to study GBM as well as to test the anti-tumoral efficacy of new drugs, their ability to interact with target cells, modulate the tumor microenvironment, cross the BBB and/or their toxicity. Prospective studies following these lines of research may lead to a better diagnosis, prognosis and treatment of patients with GBM.
- Published
- 2021
19. Modeling Cancer Using Zebrafish Xenografts: Drawbacks for Mimicking the Human Microenvironment
- Author
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Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cabezas Sáinz, Pablo, Pensado López, Alba, Sainz Anding, Bruno, Sánchez Piñón, Laura Elena, Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Cabezas Sáinz, Pablo, Pensado López, Alba, Sainz Anding, Bruno, and Sánchez Piñón, Laura Elena
- Abstract
The first steps towards establishing xenografts in zebrafish embryos were performed by Lee et al., 2005 and Haldi et al., 2006, paving the way for studying human cancers using this animal species. Since then, the xenograft technique has been improved in different ways, ranging from optimizing the best temperature for xenografted embryo incubation, testing different sites for injection of human tumor cells, and even developing tools to study how the host interacts with the injected cells. Nonetheless, a standard protocol for performing xenografts has not been adopted across laboratories, and further research on the temperature, microenvironment of the tumor or the cell–host interactions inside of the embryo during xenografting is still needed. As a consequence, current non-uniform conditions could be affecting experimental results in terms of cell proliferation, invasion, or metastasis; or even overestimating the effects of some chemotherapeutic drugs on xenografted cells. In this review, we highlight and raise awareness regarding the different aspects of xenografting that need to be improved in order to mimic, in a more efficient way, the human tumor microenvironment, resulting in more robust and accurate in vivo results
- Published
- 2020
20. Experimental Models to Study Autism Spectrum Disorders: hiPSCs, Rodents and Zebrafish
- Author
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Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Ciencias Forenses, Anatomía Patolóxica, Xinecoloxía e Obstetricia, e Pediatría, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Pensado López, Alba, Veiga Rúa, Sara, Carracedo Álvarez, Ángel María, Allegue Toscano, Catarina, Sánchez Piñón, Laura Elena, Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Ciencias Forenses, Anatomía Patolóxica, Xinecoloxía e Obstetricia, e Pediatría, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física, Pensado López, Alba, Veiga Rúa, Sara, Carracedo Álvarez, Ángel María, Allegue Toscano, Catarina, and Sánchez Piñón, Laura Elena
- Abstract
Autism Spectrum Disorders (ASD) affect around 1.5% of the global population, which manifest alterations in communication and socialization, as well as repetitive behaviors or restricted interests. ASD is a complex disorder with known environmental and genetic contributors; however, ASD etiology is far from being clear. In the past decades, many efforts have been put into developing new models to study ASD, both in vitro and in vivo. These models have a lot of potential to help to validate some of the previously associated risk factors to the development of the disorder, and to test new potential therapies that help to alleviate ASD symptoms. The present review is focused on the recent advances towards the generation of models for the study of ASD, which would be a useful tool to decipher the bases of the disorder, as well as to conduct drug screenings that hopefully lead to the identification of useful compounds to help patients deal with the symptoms of ASD
- Published
- 2020
21. Modeling cancer using zebrafish xenografts: Drawbacks for mimicking the human microenvironment
- Author
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Xunta de Galicia, Cabezas-Sainz, Pablo, Pensado-López, Alba, Sainz, Bruno Jr., Sánchez, Laura, Xunta de Galicia, Cabezas-Sainz, Pablo, Pensado-López, Alba, Sainz, Bruno Jr., and Sánchez, Laura
- Abstract
The first steps towards establishing xenografts in zebrafish embryos were performed by Lee et al., 2005 and Haldi et al., 2006, paving the way for studying human cancers using this animal species. Since then, the xenograft technique has been improved in different ways, ranging from optimizing the best temperature for xenografted embryo incubation, testing different sites for injection of human tumor cells, and even developing tools to study how the host interacts with the injected cells. Nonetheless, a standard protocol for performing xenografts has not been adopted across laboratories, and further research on the temperature, microenvironment of the tumor or the cell–host interactions inside of the embryo during xenografting is still needed. As a consequence, current non-uniform conditions could be affecting experimental results in terms of cell proliferation, invasion, or metastasis; or even overestimating the effects of some chemotherapeutic drugs on xenografted cells. In this review, we highlight and raise awareness regarding the different aspects of xenografting that need to be improved in order to mimic, in a more efficient way, the human tumor microenvironment, resulting in more robust and accurate in vivo results.
- Published
- 2020
22. Cellular and Molecular Mechanisms Underlying Glioblastoma and Zebrafish Models for the Discovery of New Treatments
- Author
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Reimunde, Pedro, primary, Pensado-López, Alba, additional, Carreira Crende, Martín, additional, Lombao Iglesias, Vanesa, additional, Sánchez, Laura, additional, Torrecilla-Parra, Marta, additional, Ramírez, Cristina M., additional, Anfray, Clément, additional, and Torres Andón, Fernando, additional
- Published
- 2021
- Full Text
- View/download PDF
23. Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells
- Author
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Cabezas-Sainz, Pablo, primary, Coppel, Carlos, additional, Pensado-López, Alba, additional, Fernandez, Pedro, additional, Muinelo-Romay, Laura, additional, López-López, Rafael, additional, Rubiolo, Juan A., additional, and Sánchez, Laura, additional
- Published
- 2021
- Full Text
- View/download PDF
24. Experimental Models to Study Autism Spectrum Disorders: hiPSCs, Rodents and Zebrafish
- Author
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Pensado-López, Alba, primary, Veiga-Rúa, Sara, additional, Carracedo, Ángel, additional, Allegue, Catarina, additional, and Sánchez, Laura, additional
- Published
- 2020
- Full Text
- View/download PDF
25. Modeling Cancer Using Zebrafish Xenografts: Drawbacks for Mimicking the Human Microenvironment
- Author
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Cabezas-Sáinz, Pablo, primary, Pensado-López, Alba, additional, Sáinz, Bruno, additional, and Sánchez, Laura, additional
- Published
- 2020
- Full Text
- View/download PDF
26. Métodos experimentales en el pez cebra (Danio rerio) para la caracterización de genes humanos relacionados con trastornos del neurodesarrollo
- Author
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Fernández Santos, Maider, Martínez Portela, Paulino, Pensado López, Alba, and Universidade de Santiago de Compostela. Facultade de Veterinaria
- Subjects
Pez Cebra ,Trastornos del neurodesarrollo ,Morfolinos ,Trip12 ,CRISPR/Cas9 ,Discapacidad intelectual ,Trastornos del Espectro Autista ,Investigación::31 Ciencias agrarias::3109 Ciencias veterinarias [Materias] - Abstract
Traballo Fin de Graorn Veterinaria. Curso 2020-2021 Los trastornos del neurodesarrollo son un grupo de trastornos que se manifiestan en períodos tempranos del desarrollo. Entre ellos se encuentran la discapacidad intelectual y los trastornos del espectro autista, que se solapan con gran frecuencia y en cuya etiología influyen factores genéticos y ambientales. Los análisis realizados para descubrir genes involucrados en la discapacidad intelectual han señalado la implicación de TRIP12, el cual también puede provocar autismo. Para investigar la implicación de TRIP12 en este tipo de trastornos, se realizó un bloqueo transitorio del gen mediante morfolinos en pez cebra, analizando el fenotipo morfológico resultante y la locomoción, y se realizó una validación de genes housekeeping así como del bloqueo transitorio a través de análisis de expresión génica por qPCR. Asimismo, se caracterizaron genotípica y fenotípicamente individuos de una línea mutante en pez cebra de trip12 generada mediante el sistema CRISPR/Cas9. Los resultados del análisis fenotípico de los individuos con bloqueo transitorio del gen revelan diferencias significativas (p < 0,05) en la longitud del cuerpo, el diámetro de la cabeza, la distancia del otolito al ojo y el área del ojo, siendo todas las medidas menores en individuos wild type. En relación al análisis fenotípico de los individuos mutantes, existe un aumento estadísticamente significativo respecto a los individuos wild type en todas las medidas (p < 0,05), a excepción del diámetro de la cabeza, que no presenta diferencias en ningún grupo. En cuanto al análisis de locomoción, se detectaron diferencias significativas (p < 0,05), presentando los individuos morfantes un menor movimiento que los wild type tanto en condiciones de luz como de oscuridad.
- Published
- 2021
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