26 results on '"Peniche, G"'
Search Results
2. Determining sex in golden eagle (Aquila chrysaetos) nestlings
- Author
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Peniche, G., primary, Shaw, D. J., additional, Dures, S. G., additional, Ciavaglia, S., additional, Thompson, D. B. A., additional, Anderson, N. E., additional, and Meredith, A. L., additional
- Published
- 2022
- Full Text
- View/download PDF
3. Estimation of milk production in hair ewes by two methods of measurement
- Author
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Irina Peniche G, Luis Sarmiento F, and Ronald Santos R
- Subjects
Hand milking ,lambs ,weighing ,Veterinary medicine ,SF600-1100 - Abstract
ABSTRACT Objective. The objective of the current study was to compare two methods to estimate daily milk production in crossbred hair ewes. Materials and methods. Eight multiparous, crossbred (Pelibuey x Katahdin) lactating hair ewes were used in a completely randomized design with repeated measurements, for 56 days. Ewes were fed ad libitum with a diet based on concentrate. Milk production was estimated twice a week for each ewe by both methods, suckled-hand (SH) and weigh-suckle-weigh (WSW) method. Results. Milk production (p>0.05) means were 031.9±95.6 and 1119.0±95.6 g/day/ewe for SH and WSW method, respectively. Conclusions. No significant differences (p>0.05) were found between methods for milk yield.
- Published
- 2015
4. Establishing haematological and biochemical reference intervals for free-ranging Scottish golden eagle nestlings (Aquila chrysaetos)
- Author
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Peniche G., Shaw D.J., Thompson D.B.A., Brain J.C., Reid R., Weston E., Benn S., Anderson D., Grant J., Pate L., Anderson N.E., and Meredith A.L.
- Subjects
Nestling ,haematology ,biochemistry ,reference interval ,Management, Monitoring, Policy and Law ,golden eagle ,Ecology, Evolution, Behavior and Systematics ,Nature and Landscape Conservation - Abstract
Health assessment of individuals is an important aspect of monitoring endangered wildlife populations. Haematological and biochemical values are a common health assessment tool, and whilst reference values are well established for domestic species, they are often not available for wild animal species. This study established 31 haematological and biochemical reference intervals for golden eagle (Aquila chrysaetos) nestlings in Scotland, in order to improve the understanding of the species’ health and support conservation efforts. Reference intervals were created from 47 nestlings (ages 2–7.5 weeks old) across 37 nests, to date, the largest sample of wild individuals of this species and age cohort sampled for these purposes. Upper reference intervals for concentrations of lymphocytes, total protein, cholesterol, triglycerides, uric acid, and monocytes, calculated in this study, are higher than those found for adult raptors and the interval span is higher than that observed in adult raptors for concentrations of AST, albumin, eosinophil, LDH, and monocyte count. Statistically significant positive correlations were found with age and concentrations of haemoglobin, lymphocytes, serum pH, and creatine kinase, and significant negative correlations with age for concentrations of thrombocytes, heterophils, total protein, globulin, and lactate dehydrogenase. Packed cell volume was significantly higher for females than males, and concentration of calcium and eosinophils were higher for individuals in good body condition than those in moderate body condition. The reference intervals produced by this study will be of important use to the veterinary and conservation management communities and will aid the long-term monitoring of the Scottish golden eagle population health.
- Published
- 2022
5. Establishing haematological and biochemical reference intervals for free-ranging Scottish golden eagle nestlings (Aquila chrysaetos)
- Author
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Peniche, G, Shaw, DJ, Thompson, DBA, Brain, JC, Reid, R, Weston, E, Benn, S, Anderson, D, Grant, J, Pate, L, Anderson, NE, Meredith, AL, Peniche, G, Shaw, DJ, Thompson, DBA, Brain, JC, Reid, R, Weston, E, Benn, S, Anderson, D, Grant, J, Pate, L, Anderson, NE, and Meredith, AL
- Abstract
Health assessment of individuals is an important aspect of monitoring endangered wildlife populations. Haematological and biochemical values are a common health assessment tool, and whilst reference values are well established for domestic species, they are often not available for wild animal species. This study established 31 haematological and biochemical reference intervals for golden eagle (Aquila chrysaetos) nestlings in Scotland, in order to improve the understanding of the species’ health and support conservation efforts. Reference intervals were created from 47 nestlings (ages 2–7.5 weeks old) across 37 nests, to date, the largest sample of wild individuals of this species and age cohort sampled for these purposes. Upper reference intervals for concentrations of lymphocytes, total protein, cholesterol, triglycerides, uric acid, and monocytes, calculated in this study, are higher than those found for adult raptors and the interval span is higher than that observed in adult raptors for concentrations of AST, albumin, eosinophil, LDH, and monocyte count. Statistically significant positive correlations were found with age and concentrations of haemoglobin, lymphocytes, serum pH, and creatine kinase, and significant negative correlations with age for concentrations of thrombocytes, heterophils, total protein, globulin, and lactate dehydrogenase. Packed cell volume was significantly higher for females than males, and concentration of calcium and eosinophils were higher for individuals in good body condition than those in moderate body condition. The reference intervals produced by this study will be of important use to the veterinary and conservation management communities and will aid the long-term monitoring of the Scottish golden eagle population health.
- Published
- 2022
6. Disease Risk Analysis and Post‐Release Health Surveillance for a Reintroduction Programme: the Pool Frog Pelophylax lessonae
- Author
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Sainsbury, A. W., Yu‐Mei, R., Ågren, E., Vaughan‐Higgins, R. J., Mcgill, I. S., Molenaar, F., Peniche, G., and Foster, J.
- Published
- 2017
- Full Text
- View/download PDF
7. EVALUACIÓN HISTOLÓGICA DE LA REPARACIÓN TISULAR POST PALATECTOMÍA PARCIAL EQUINA
- Author
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Hamilton Escobar B, Irina Peniche G, Leonardo Álvarez A, and Mastoby Martínez M
- Subjects
Palatitis ,Equinos ,Palatectomía ,Reparación tisular. ,Veterinary medicine ,SF600-1100 - Abstract
Objetivo. Evaluar la reparación tisular posterior a la realización de técnicas de palatectomía parcial,como tratamiento de la palatitis en equinos. Materiales y métodos. Se utilizaron 12 caballos,divididos aleatoriamente en dos grupos, ambos con seis animales, a los del primer grupo se lespracticó palatectomía parcial con suturación y a los del segundo grupo, se les realizó palatectomíaparcial y luego cauterización. Los grupos fueron divididos en tres subgrupos de dos animales cadauno. A los primeros subgrupos, suturados y cauterizados, se les tomó biopsia el día 5; a los segundossubgrupos, el día 15 y a los terceros el día 30 posquirúrgico. Resultados. El día 5 posquirúrgico,en ambos grupos se observó la presencia de infiltrado celular inflamatorio de tipo mononuclear(macrófago), inicio del proceso de epitelización, proliferación fibroblástica y formación de nuevosvasos sanguíneos; notándose severa ulceración del epitelio con abundantes detritos celulares soloen los animales cauterizados. El día 15 posquirúrgico, en los animales suturados, se observó infiltradocelular mononuclear, proliferación epitelial y fibroblástica, y presencia de nuevos vasos sanguíneos.Las muestras de los animales cauterizados, presentaron además, ulceración epitelial, detrituscelulares y formación de tejido de granulación desorganizado. El día 30 posquirúrgico, los animalessuturados, no mostraron inflamación evidente, el epitelio se observó con características normalesaunque más grueso que el adyacente. En los animales cauterizados, no se observó infiltrado celularmononuclear, aunque continuaba la ulceración epitelial, igualmente existía, proliferaciónfibroblástica desorganizada y abundantes fibras colágenas. Conclusión. Es posible concluir que elproceso de reparación tisular se vio favorecido por la técnica de palatectomía parcial con suturación.
- Published
- 2006
8. The genome sequence of the European golden eagle, Aquila chrysaetos chrysaetos Linnaeus 1758.
- Author
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Mead, D, Ogden, R, Meredith, A, Peniche, G, Smith, M, Corton, C, Oliver, K, Skelton, J, Betteridge, E, Doulcan, J, Holmes, N, Wright, V, Loose, M, Quail, MA, McCarthy, SA, Howe, K, Chow, W, Torrance, J, Collins, J, Challis, R, Durbin, R, Blaxter, M, Mead, D, Ogden, R, Meredith, A, Peniche, G, Smith, M, Corton, C, Oliver, K, Skelton, J, Betteridge, E, Doulcan, J, Holmes, N, Wright, V, Loose, M, Quail, MA, McCarthy, SA, Howe, K, Chow, W, Torrance, J, Collins, J, Challis, R, Durbin, R, and Blaxter, M
- Abstract
We present a genome assembly from an individual female Aquila chrysaetos chrysaetos (the European golden eagle; Chordata; Aves; Accipitridae). The genome sequence is 1.23 gigabases in span. The majority of the assembly is scaffolded into 28 chromosomal pseudomolecules, including the W and Z sex chromosomes.
- Published
- 2021
9. Evaluacion histologica de la reparacion tisular post palatectomia parcial equina
- Author
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Escobar B., Hamilton, Peniche G., Irina, Alvarez A., Leonardo, and MartÃnez M., Mastoby
- Published
- 2006
10. Long-term health effects of harness-mounted radio transmitters in red kites (Milvus milvus) in England
- Author
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Peniche, G., Vaughan-Higgins, R., Carter, Ian, Pocknell, A., Simpson, D., and Sainsbury, A.
- Published
- 2011
- Full Text
- View/download PDF
11. Eimeria spp. in captive-reared corncrakes (Crex crex): results of GeneScan assay consistent with high prevalence of infection and extra-intestinal life stages
- Author
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Serna, H, Pocknell, A, Sainsbury, A W, Peniche, G, Blake, D P, and Beckmann, K M
- Subjects
parasitic diseases - Abstract
Eimeria crecis and Eimeria nenei have been detected in association with enteric disease (‘coccidiosis’) in the corncrake (Crex crex: Family Rallidae, Order Gruiformes). Both parasite species are common in apparently healthy free-living corncrakes, but captive-bred juvenile birds reared for reintroduction appeared particularly susceptible to clinical coccidiosis. We investigated the occurrence and relative pathogenicity of these Eimeria species in this juvenile corncrake population and developed a diagnostic species-specific polymerase chain reaction (PCR) for their identification. PCR amplification and sequencing of 18S rDNA was performed on genomic DNA extracted from samples of corncrake intestine, liver and spleen. Sequences generated were used to design a GeneScan diagnostic PCR assay targeting a species-specific TTA indel located within the 18S rDNA – the results suggested this assay was more sensitive than the 18S rDNA/amplicon sequencing approach. Eimeria sp. DNA (consistent with Eimeria sp. infection) was detected at a high prevalence and E. crecis was the predominant species. Each Eimeria species was detected in cases with and without histological evidence of coccidiosis: parasite detection was not statistically associated with disease. In addition to intestinal tissue, liver and spleen samples were positive for Eimeria sp. DNA. Its detection in tissues other than intestine is unusual and a novel finding in corncrakes, although extra-intestinal infection occurs with closely-related Eimeria species in cranes (Family Gruidae, Order Gruiformes). Eimeria sp. infection of corncrakes appears typically to be chronic, and to exhibit extra-intestinal spread: as in cranes, these characteristics may be adaptations to the host’s migratory nature.
- Published
- 2018
12. Estimation of milk production in hair ewes by two methods of measurement
- Author
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Luis Sarmiento F, Irina Peniche G, and Ronald Santos R
- Subjects
lcsh:Veterinary medicine ,General Veterinary ,Hand milking ,Aquatic Science ,Biology ,Milk production ,AGROVOC) ,lambs ,Crossbreed ,weighing ,Animal science ,Milk yield ,lcsh:SF600-1100 ,Animal Science and Zoology ,weighing (Source ,Veterinaria ,Completely randomized design - Abstract
Objective. The objective of the current study was to compare two methods to estimate daily milk production in crossbred hair ewes. Materials and methods. Eight multiparous, crossbred (Pelibuey x Katahdin) lactating hair ewes were used in a completely randomized design with repeated measurements, for 56 days. Ewes were fed ad libitum with a diet based on concentrate. Milk production was estimated twice a week for each ewe by both methods, suckled-hand (SH) and weigh-suckle-weigh (WSW) method. Results. Milk production (p>0.05) means were 1031.9±95.6 and 1119.0±95.6 g/day/ewe for SH and WSW method, respectively. Conclusions. No significant differences (p>0.05) were found between methods for milk yield.RESUMENObjetivo. El objetivo del presente trabajo fue comparar dos métodos para estimar la producción diaria de leche en ovejas de pelo cruzadas. Materiales y métodos. Se utilizaron ocho ovejas multíparas, cruzadas (Pelibuey x Katahdin) y lactantes, en un diseño completamente al azar, con medidas repetidas, durante 56 días. Las ovejas fueron alimentadas ad libitum con una dieta basada en concentrado. La producción de leche se estimó dos veces a la semana a cada oveja, mediante ambos métodos, el doble pesaje del cordero-ordeño manual de la ovejas (DPC-OMO) y el doble pesaje del cordero (DPC). Resultados. La producción de leche estimada (p>0.05) fue de 1031.9±95.6 y 1119.0±95.6 g/día/ovejas para el DPC-OMO y DPC, respectivamente. Conclusión. No se encontraron diferencias significativas (p>0.05) entre métodos de estimación de la producción de leche.
- Published
- 2015
13. Disease risk analysis and post-release health surveillance for a reintroduction programme: the pool frog Pelophylax lessonae
- Author
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Sainsbury, A W, Chang, Y M, Ågren, E, Vaughan-Higgins, R J, McGill, I S, Molenaar, F, Peniche, G, and Foster, J
- Abstract
There are risks from disease in undertaking wild animal reintroduction programmes. Methods of disease risk analysis have been advocated to assess and mitigate these risks, and post‐release health and disease surveillance can be used to assess the effectiveness of the disease risk analysis, but results for a reintroduction programme have not to date been recorded. We carried out a disease risk analysis for the reintroduction of pool frogs (Pelophylax lessonae) to England, using information gained from the literature and from diagnostic testing of Swedish pool frogs and native amphibians. Ranavirus and Batrachochytrium dendrobatidis were considered high‐risk disease threats for pool frogs at the destination site. Quarantine was used to manage risks from disease due to these two agents at the reintroduction site: the quarantine barrier surrounded the reintroduced pool frogs. Post‐release health surveillance was carried out through regular health examinations of amphibians in the field at the reintroduction site and collection and examination of dead amphibians. No significant health or disease problems were detected, but the detection rate of dead amphibians was very low. Methods to detect a higher proportion of dead reintroduced animals and closely related species are required to better assess the effects of reintroduction on health and disease.
- Published
- 2017
14. Estimation of milk production in hair ewes by two methods of measurement
- Author
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Peniche G, Irina, Sarmiento F, Luis, and Santos R, Ronald
- Subjects
ordeño manual ,weighing ,Corderos ,Hand milking ,pesaje ,lambs - Abstract
Objective. The objective of the current study was to compare two methods to estimate daily milk production in crossbred hair ewes. Materials and methods. Eight multiparous, crossbred (Pelibuey x Katahdin) lactating hair ewes were used in a completely randomized design with repeated measurements, for 56 days. Ewes were fed ad libitum with a diet based on concentrate. Milk production was estimated twice a week for each ewe by both methods, suckled-hand (SH) and weigh-suckle-weigh (WSW) method. Results. Milk production (p>0.05) means were 1031.9±95.6 and 1119.0±95.6 g/day/ewe for SH and WSW method, respectively. Conclusions. No significant differences (p>0.05) were found between methods for milk yield. Objetivo. El objetivo del presente trabajo fue comparar dos métodos para estimar la producción diaria de leche en ovejas de pelo cruzadas. Materiales y métodos. Se utilizaron ocho ovejas multíparas, cruzadas (Pelibuey x Katahdin) y lactantes, en un diseño completamente al azar, con medidas repetidas, durante 56 días. Las ovejas fueron alimentadas ad libitum con una dieta basada en concentrado. La producción de leche se estimó dos veces a la semana a cada oveja, mediante ambos métodos, el doble pesaje del cordero-ordeño manual de la ovejas (DPC-OMO) y el doble pesaje del cordero (DPC). Resultados. La producción de leche estimada (p>0.05) fue de 1031.9±95.6 y 1119.0±95.6 g/día/ovejas para el DPC-OMO y DPC, respectivamente. Conclusión. No se encontraron diferencias significativas (p>0.05) entre métodos de estimación de la producción de leche.
- Published
- 2015
15. Disease risk analysis and post-release health surveillance for a reintroduction programme: The pool frog Pelophylax lessonae
- Author
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Sainsbury, A.W., Yu-Mei, R., Ågren, E., Vaughan-Higgins, R.J., Mcgill, I.S., Molenaar, F., Peniche, G., Foster, J., Sainsbury, A.W., Yu-Mei, R., Ågren, E., Vaughan-Higgins, R.J., Mcgill, I.S., Molenaar, F., Peniche, G., and Foster, J.
- Abstract
There are risks from disease in undertaking wild animal reintroduction programmes. Methods of disease risk analysis have been advocated to assess and mitigate these risks, and post-release health and disease surveillance can be used to assess the effectiveness of the disease risk analysis, but results for a reintroduction programme have not to date been recorded. We carried out a disease risk analysis for the reintroduction of pool frogs (Pelophylax lessonae) to England, using information gained from the literature and from diagnostic testing of Swedish pool frogs and native amphibians. Ranavirus and Batrachochytrium dendrobatidis were considered high-risk disease threats for pool frogs at the destination site. Quarantine was used to manage risks from disease due to these two agents at the reintroduction site: the quarantine barrier surrounded the reintroduced pool frogs. Post-release health surveillance was carried out through regular health examinations of amphibians in the field at the reintroduction site and collection and examination of dead amphibians. No significant health or disease problems were detected, but the detection rate of dead amphibians was very low. Methods to detect a higher proportion of dead reintroduced animals and closely related species are required to better assess the effects of reintroduction on health and disease.
- Published
- 2016
16. Disease Risk Analysis and Post-Release Health Surveillance for a Reintroduction Programme: the Pool FrogPelophylax lessonae
- Author
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Sainsbury, A. W., primary, Yu-Mei, R., additional, Ågren, E., additional, Vaughan-Higgins, R. J., additional, Mcgill, I. S., additional, Molenaar, F., additional, Peniche, G., additional, and Foster, J., additional
- Published
- 2016
- Full Text
- View/download PDF
17. Estimation of milk production in hair ewes by two methods of measurement
- Author
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Peniche G., Irina, Sarmiento Franco, Luis, Santos Ricalde, Ronald, Peniche G., Irina, Sarmiento Franco, Luis, and Santos Ricalde, Ronald
- Abstract
Objective. The objective of the current study was to compare two methods to estimate daily milk production in crossbred hair ewes. Materials and methods. Eight multiparous, crossbred (Pelibuey x Katahdin) lactating hair ewes were used in a completely randomized design with repeated measurements, for 56 days. Ewes were fed ad libitum with a diet based on concentrate. Milk production was estimated twice a week for each ewe by both methods, suckled-hand (SH) and weigh-suckle-weigh (WSW) method. Results. Milk production (p>0.05) means were 1031.9±95.6 and 1119.0±95.6 g/day/ewe for SH and WSW method, respectively. Conclusions. No significant differences (p>0.05) were found between methods for milk yield., Objetivo. El objetivo del presente trabajo fue comparar dos métodos para estimar la producción diaria de leche en ovejas de pelo cruzadas. Materiales y métodos. Se utilizaron ocho ovejas multíparas, cruzadas (Pelibuey x Katahdin) y lactantes, en un diseño completamente al azar, con medidas repetidas, durante 56 días. Las ovejas fueron alimentadas ad libitum con una dieta basada en concentrado. La producción de leche se estimó dos veces a la semana a cada oveja, mediante ambos métodos, el doble pesaje del cordero-ordeño manual de la ovejas (DPC-OMO) y el doble pesaje del cordero (DPC). Resultados. La producción de leche estimada (p>0.05) fue de 1031.9±95.6 y 1119.0±95.6 g/día/ovejas para el DPC-OMO y DPC, respectivamente. Conclusión. No se encontraron diferencias significativas (p>0.05) entre métodos de estimación de la producción de leche.
- Published
- 2015
18. Estimation of milk production in hair ewes by two methods of measurement
- Author
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Peniche G, Irina, primary, Sarmiento F, Luis, additional, and Santos R, Ronald, additional
- Published
- 2015
- Full Text
- View/download PDF
19. Evaluación histológica de la reparación tisular posterior a la realización de técnicas de palatectomía parcial equina
- Author
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Irina Peniche G, Hamilton Escobar B, Mastoby Martínez M, and Leonardo Álvarez A
- Subjects
lcsh:Veterinary medicine ,General Veterinary ,lcsh:SF600-1100 ,Animal Science and Zoology ,Palatitis ,tissue repair ,equine ,palectomy ,Aquatic Science - Abstract
Objetivo. Evaluar la reparación tisular posterior a la realización de técnicas de palatectomía parcial, como tratamiento de la palatitis en equinos. Materiales y métodos. Se utilizaron 12 caballos, divididos aleatoriamente en dos grupos, ambos con seis animales, a los del primer grupo se les practicó palatectomía parcial con suturación y a los del segundo grupo, se les realizó palatectomía parcial y luego cauterización. Los grupos fueron divididos en tres subgrupos de dos animales cada uno. A los primeros subgrupos, suturados y cauterizados, se les tomó biopsia el día 5; a los segundos subgrupos, el día 15 y a los terceros el día 30 posquirúrgico. Resultados. El día 5 posquirúrgico, en ambos grupos se observó la presencia de infiltrado celular inflamatorio de tipo mononuclear (macrófago), inicio del proceso de epitelización, proliferación fibroblástica y formación de nuevos vasos sanguíneos; notándose severa ulceración del epitelio con abundantes detritos celulares solo en los animales cauterizados. El día 15 posquirúrgico, en los animales suturados, se observó infiltrado celular mononuclear, proliferación epitelial y fibroblástica, y presencia de nuevos vasos sanguíneos. Las muestras de los animales cauterizados, presentaron además, ulceración epitelial, detritus celulares y formación de tejido de granulación desorganizado. El día 30 posquirúrgico, los animales suturados, no mostraron inflamación evidente, el epitelio se observó con características normales aunque más grueso que el adyacente. En los animales cauterizados, no se observó infiltrado celular mononuclear, aunque continuaba la ulceración epitelial, igualmente existía, proliferación fibroblástica desorganizada y abundantes fibras colágenas. Conclusión. Es posible concluir que el proceso de reparación tisular se vio favorecido por la técnica de palatectomía parcial con suturación.
- Published
- 2006
20. EVALUACIÓN HISTOLÓGICA DE LA REPARACIÓN TISULAR POST PALATECTOMÍA PARCIAL EQUINA
- Author
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Escobar B, Hamilton, Peniche G, Irina, Álvarez A, Leonardo, and Martínez M, Mastoby
- Subjects
reparación tisular ,palatectomía ,equinos ,Palatitis ,tissue repair ,palectomy ,equine - Abstract
Objetivo. Evaluar la reparación tisular posterior a la realización de técnicas de palatectomía parcial, como tratamiento de la palatitis en equinos. Materiales y métodos. Se utilizaron 12 caballos, divididos aleatoriamente en dos grupos, ambos con seis animales, a los del primer grupo se les practicó palatectomía parcial con suturación y a los del segundo grupo, se les realizó palatectomía parcial y luego cauterización. Los grupos fueron divididos en tres subgrupos de dos animales cada uno. A los primeros subgrupos, suturados y cauterizados, se les tomó biopsia el día 5; a los segundos subgrupos, el día 15 y a los terceros el día 30 posquirúrgico. Resultados. El día 5 posquirúrgico, en ambos grupos se observó la presencia de infiltrado celular inflamatorio de tipo mononuclear (macrófago), inicio del proceso de epitelización, proliferación fibroblástica y formación de nuevos vasos sanguíneos; notándose severa ulceración del epitelio con abundantes detritos celulares solo en los animales cauterizados. El día 15 posquirúrgico, en los animales suturados, se observó infiltrado celular mononuclear, proliferación epitelial y fibroblástica, y presencia de nuevos vasos sanguíneos. Las muestras de los animales cauterizados, presentaron además, ulceración epitelial, detritus celulares y formación de tejido de granulación desorganizado. El día 30 posquirúrgico, los animales suturados, no mostraron inflamación evidente, el epitelio se observó con características normales aunque más grueso que el adyacente. En los animales cauterizados, no se observó infiltrado celular mononuclear, aunque continuaba la ulceración epitelial, igualmente existía, proliferación fibroblástica desorganizada y abundantes fibras colágenas. Conclusión. Es posible concluir que el proceso de reparación tisular se vio favorecido por la técnica de palatectomía parcial con suturación. Objective. To evaluate the tissue repair posterior to realization of technical partial paladin, as treatment of palatitis in equine. Materials and methods. 12 horses were used, divided aleatorily in two groups, both with six animals, to those of first group were practiced partial paladin with saturation and to those of the second group, partial paladin were practiced and then cauterization. The groups were divided in three subgroups of two animals each one. To the first ones sutured and cauterized, biopsy were taken the day 5; to the second ones the day 15 and at the third ones the day 30 post surgeon. Results. The day 5 post surgeon, in both groups the presence of cellular infiltrate inflammatory was observed of mononuclear type, initiation of the epithelization process, fibroblastic proliferation and formation of new sanguine vessels; being noticed severe ulceration of the epithelium with abundant cellular detrituses in the cauterized animals. The day 15 post surgeon, in the sutured animals was observed infiltrated cellular mononuclear, epithelial proliferation and fibroblastic, and presence of new sanguine vessels. Samples of the cauterized animals also presented epithelial ulceration, cellular detritus and formation of disorganized granulation tissue. The day 30 post surgeon the sutured animals didn’t show evident inflammation, the epithelium was observed with normal characteristics although thicker that the adjacent one. In the cauterized animals, infiltrated cellular mononuclear was not observed, although the epithelial ulceration continued, equally existed disorganized fibroblastic proliferation and abundant collagen fibers. Conclusion. It is possible to conclude that the process of tissue repair was favored by the technique of partial paladin with suturation.
- Published
- 2006
21. Mercury speciation in Scottish raptors reveals high proportions of inorganic mercury in Scottish golden eagles (Aquila chrysaetos): Potential occurrence of mercury selenide nanoparticles.
- Author
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Lancaster ST, Peniche G, Alzahrani A, Blanz M, Newton J, Taggart MA, Corns WT, Krupp EM, and Feldmann J
- Subjects
- Animals, Isotopes, Propylamines, Sulfides, Eagles, Mercury analysis, Methylmercury Compounds, Nanoparticles, Raptors, Strigiformes
- Abstract
Knowledge of the uptake and fate of mercury (Hg) compounds in biota is important in understanding the global cycling of Hg and its transfer pathways through food chains. In this study, we analysed total mercury (T-Hg) and methylmercury (MeHg) concentrations in 117 livers of Scottish birds of prey that were found across Scotland and submitted for post-mortem examination through the Raptor Health Scotland project between 2009 and 2019. Statistical comparisons focussed on six species (barn owl, Tyto alba; Eurasian common buzzard, Buteo buteo; golden eagle, Aquila chrysaetos; hen harrier, Circus cyaneus; Eurasian sparrowhawk, Accipiter nisus; and tawny owl, Strix aluco) and showed that golden eagles had a statistically lower fraction of MeHg compared to other raptor species. Further investigation using stable carbon and stable nitrogen isotope ratio measurements carried out for the golden eagles (n = 15) indicated that the increased presence of inorganic mercury (iHg) correlated with a marine influence on the primarily terrestrial diet. Additional bioimaging (n = 1) with laser ablation - inductively coupled plasma - mass spectrometry indicated the co-location of Hg and selenium (Se) within the liver tissue and transmission electron microscopy showed evidence of nanoparticles within the range of 10-20 nm. Further analysis using single particle - inductively coupled plasma - mass spectrometry (n = 4) confirmed the presence of Hg nanoparticles. Together, the evidence suggests the presence of mercury selenide (HgSe) nanoparticles in the liver of some golden eagles that, to our knowledge, has never been directly observed in terrestrial birds of prey. This study points to two alternative hypotheses: these golden eagles may be efficient at breaking down MeHg and form HgSe nanoparticles as a detoxification mechanism (as previously observed in cetaceans), or some golden eagles with elevated iHg may have accumulated these nanoparticles by foraging on stranded cetaceans or seabirds., Competing Interests: Declaration of competing interest There are no conflicts to declare., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
22. The genome sequence of the European golden eagle, Aquila chrysaetos chrysaetos Linnaeus 1758.
- Author
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Mead D, Ogden R, Meredith A, Peniche G, Smith M, Corton C, Oliver K, Skelton J, Betteridge E, Doulcan J, Holmes N, Wright V, Loose M, Quail MA, McCarthy SA, Howe K, Chow W, Torrance J, Collins J, Challis R, Durbin R, and Blaxter M
- Abstract
We present a genome assembly from an individual female Aquila chrysaetos chrysaetos (the European golden eagle; Chordata; Aves; Accipitridae). The genome sequence is 1.23 gigabases in span. The majority of the assembly is scaffolded into 28 chromosomal pseudomolecules, including the W and Z sex chromosomes., Competing Interests: No competing interests were disclosed., (Copyright: © 2021 Mead D et al.)
- Published
- 2021
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23. Concentration and origin of lead (Pb) in liver and bone of Eurasian buzzards (Buteo buteo) in the United Kingdom.
- Author
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Taggart MA, Shore RF, Pain DJ, Peniche G, Martinez-Haro M, Mateo R, Homann J, Raab A, Feldmann J, Lawlor AJ, Potter ED, Walker LA, Braidwood DW, French AS, Parry-Jones J, Swift JA, and Green RE
- Subjects
- Animals, Europe, Humans, Liver, United Kingdom, Birds, Lead
- Abstract
Ingestion of lead (Pb) derived from ammunition used in the hunting of game animals is recognised to be a significant potential source of Pb exposure of wild birds, including birds of prey. However, there are only limited data for birds of prey in Europe regarding tissue concentrations and origins of Pb. Eurasian buzzards (Buteo buteo) found dead in the United Kingdom during an 11-year period were collected and the concentrations of Pb in the liver and femur were measured. Concentrations in the liver consistent with acute exposure to Pb were found in 2.7% of birds and concentration in the femur consistent with exposure to lethal levels were found in 4.0% of individuals. Pb concentration in the femur showed no evidence of consistent variation among or within years, but was greater for old than for young birds. The Pb concentration in the liver showed no effect of the birds' age, but varied markedly among years and showed a consistent tendency to increase substantially within years throughout the UK hunting season for gamebirds. The resemblance of the stable isotope composition of Pb from buzzard livers to that of Pb from the types of shotgun ammunition most widely-used in the UK increased markedly with increasing Pb concentration in the liver. Stable isotope results were consistent with 57% of the mass of Pb in livers of all of the buzzards sampled being derived from shotgun pellets, with this proportion being 89% for the birds with concentrations indicating acute exposure to Pb. Hence, most of the Pb acquired by Eurasian buzzards which have liver concentrations likely to be associated with lethal and sublethal effects is probably obtained when they prey upon or scavenge gamebirds and mammals shot using Pb shotgun pellets., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
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- 2020
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24. Eimeria spp. in captive-reared corncrakes (Crex crex): results of a GeneScan assay consistent with high prevalence of infection and extra-intestinal life stages.
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Serna H, Pocknell A, Sainsbury AW, Peniche G, Blake DP, and Beckmann KM
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- Animals, Bird Diseases parasitology, Birds, Coccidiosis epidemiology, Coccidiosis parasitology, DNA, Protozoan analysis, Intestines parasitology, Polymerase Chain Reaction veterinary, Prevalence, Species Specificity, Bird Diseases epidemiology, Coccidiosis veterinary, Eimeria isolation & purification
- Abstract
Eimeria crecis and Eimeria nenei have been detected in association with enteric disease ("coccidiosis") in the corncrake (Crex crex: Family Rallidae, Order Gruiformes). Both parasite species are common in apparently healthy free-living corncrakes, but captive-bred juvenile birds reared for reintroduction appeared particularly susceptible to clinical coccidiosis. We investigated the occurrence and relative pathogenicity of these Eimeria species in this juvenile corncrake population and developed a diagnostic species-specific polymerase chain reaction (PCR) for their identification. PCR amplification and sequencing of 18S rDNA were performed on genomic DNA extracted from samples of corncrake intestine, liver and spleen. Sequences generated were used to design a GeneScan diagnostic PCR assay targeting a species-specific TTA indel located within the 18S rDNA - the results suggested this assay was more sensitive than the 18S rDNA/amplicon sequencing approach. Eimeria sp. DNA (consistent with Eimeria sp. infection) was detected at a high prevalence and E. crecis was the predominant species. Each Eimeria species was detected in cases with and without histological evidence of coccidiosis: parasite detection was not statistically associated with disease. In addition to intestinal tissue, liver and spleen samples were positive for Eimeria sp. DNA. Its detection in tissues other than intestine is unusual and a novel finding in corncrakes, although extra-intestinal infection occurs with closely related Eimeria species in cranes (Family Gruidae, Order Gruiformes). Eimeria sp. infection of corncrakes appears typically to be chronic, and to exhibit extra-intestinal spread: as in cranes, these characteristics may be adaptations to the host's migratory nature.
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- 2018
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25. Protecting Free-Living Dormice: Molecular Identification of Cestode Parasites in Captive Dormice (Muscardinus avellanarius) Destined for Reintroduction.
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Peniche G, Olson PD, Bennett DJ, Wong L, Sainsbury AW, and Durrant C
- Subjects
- Animals, Cestoda genetics, England, Parasites, Polymerase Chain Reaction, Cestoda pathogenicity, Myoxidae parasitology
- Abstract
The success of any population translocation programme relies heavily on the measures implemented to control and monitor the spread of disease. Without these measures, programmes run the risk of releasing immunologically naïve species or, more dangerously, introducing novel infectious agents to native populations. As a precaution, a reintroduction programme for the common or hazel dormouse, Muscardinus avellanarius, in England screens dormice before release following captive breeding. Using PCR sequencing of a range of genes, we tested whether the same species of tapeworm(s) were present in captive and free-living dormice. Whilst only Rodentolepis straminea were identified in free-living dormice, cestode ova found in a captive individual produced a molecular match closely related to Hymenolepis microstoma and a previously unrecorded Rodentolepis species. To prevent putting at risk the free-living population, we recommended the continued treatment of dormice showing tapeworm infection before release. Our work demonstrates how molecular techniques can be used to inform reintroduction programmes, reduce risk from disease and increase chances of reintroduction success.
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- 2017
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26. Genetic structure of introduced populations: 120-year-old DNA footprint of historic introduction in an insular small mammal population.
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Simpson S, Blampied N, Peniche G, Dozières A, Blackett T, Coleman S, Cornish N, and Groombridge JJ
- Abstract
Wildlife populations have been introduced to new areas by people for centuries, but this human-mediated movement can disrupt natural patterns of genetic structure by altering patterns of gene flow. Insular populations are particularly prone to these influences due to limited opportunities for natural dispersal onto islands. Consequently, understanding how genetic patterns develop in island populations is important, particularly given that islands are frequently havens for protected wildlife. We examined the evolutionary origins and extent of genetic structure within the introduced island population of red squirrels (Sciurus vulgaris) on the Channel Island of Jersey using mitochondrial DNA (mtDNA) control region sequence and nuclear microsatellite genotypes. Our findings reveal two different genetic origins and a genetic architecture reflective of the introductions 120 years ago. Genetic structure is marked within the maternally inherited mtDNA, indicating slow dispersal of female squirrels. However, nuclear markers detected only weak genetic structure, indicating substantially greater male dispersal. Data from both mitochondrial and nuclear markers support historic records that squirrels from England were introduced to the west of the island and those from mainland Europe to the east. Although some level of dispersal and introgression across the island between the two introductions is evident, there has not yet been sufficient gene flow to erase this historic genetic "footprint." We also investigated if inbreeding has contributed to high observed levels of disease, but found no association. Genetic footprints of introductions can persist for considerable periods of time and beyond traditional timeframes of wildlife management.
- Published
- 2013
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