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In this paper, the flow characteristics of the polishing fluid between the polishing pad and the workpiece are studied for the robotic wet polishing process, and the distribution of the polishing fluid radial velocity Ur and the liquid film thickness z at different rotating radii r are revealed. The two-dimensional computational domain consisting of the polishing pad surface, the workpiece wall and the polishing fluid is established. The particle-liquid two-phase flow simulation is carried out in Fluent, and the influence of different rotation rate ω of the polishing pad and different robot swing speeds v2 on the change and distribution of polishing fluid flow rate and temperature are elaborated. The position distribution of the abrasive particles in the wet polishing process and the velocity distribution of particles in the x and y directions impacting on the workpiece surface are simulated and analyzed for polishing fluids with different average abrasive diameters dp. The three-dimensional calculation domain for wet polishing is established; the workpiece surface erosion is simulated in Fluent; the material removal rate MRR and standard deviation of material removal σ on the workpiece surface are calculated considering different combinations of polishing fluid properties Ci and polishing kinematics Pi. Under the same process parameters, the material removal rate test value MRRT and the standard deviation of material removal test value σT are compared with the simulated values, respectively. The results show that under the combination of 64 groups of physical parameters C1-C64 of the polishing fluid, the error between the test value MRRT, σT and the simulation value MRR, σ is within 5%. With 64 sets of polishing kinematics parameters P1-P64, the average error between the test value MRRT and the simulated value MRR is 4.19%. However, when the polishing pad rotation rate ω is high, there is an inefficient polishing area in the smaller radius from the polishing pad rotation center, which results in a lower MRRT in some tests than that in simulation, with an maximum error of 8.1%. The average error between the test value σT and the simulation value σ is 3.77%. When the pressure P of the polishing pad is high, the large particles embedded in the polishing pad surface follow its rotation, causing deep scratches on the workpiece surface, which results in a larger σT in some tests, with an maximum error of 7.8%. In conclusion, the material removal principle and the influence of different process parameters in the robotic wet polishing process are revealed in this paper.through modeling and simulation of the particle-liquid two-phase flow, giving an accurate estimation of the material removal rate of the robotic wet polishing process.

Abdominal aortic aneurysm (AAA) is a severe life-threatening disease that is generally asymptomatic and is diagnosed at a very late stage. The genetic component underpinning AAA is considerable, with an estimated heritability of up to 70%. Therefore, identifying genetic biomarkers for AAA is valuable for predicting high-risk populations. We used integrative bioinformatics and cellular AAA model-based validation to reveal that the gene encoding protein tyrosine phosphatase non-receptor type 22 (

Aimed at the problems of navigation in distributed environment of a mobile robot, a regionalization vision navigation method based on deep reinforcement learning is proposed. First, considering the characteristics of distributed environment, the independent submodule learning control strategy is used in different regions and the regionalization model is built to switch and combine navigation control strategies. Then, in order to make the robot have a better goal-oriented behavior, reward prediction task is integrated into the submodule, and reward sequence is played back in combination with the experience pool. Finally, depth limitation is added to the primitive exploration strategy to prevent the traversal stagnation caused by collision. The results show that the application of reward prediction and depth obstacle avoidance is helpful to improve navigation performance. In the process of multi-area environment test, the regionalization model shows the advantages that the single model does not have in terms of training time and rewards, indicating that it can better deal with large-scale navigation. In addition, the experiment is conducted in the first-person 3D environment, and the state is partially observable, which is conducive to practical application.

The aim of this study was to explore the pivotal genes or lncRNAs involved in the progression of atrial fibrillation (AF) -valvular heart disease (VHD). The mRNA profiling GSE113013 was obtained from the Gene Expression Omnibus database. The identification of differentially expressed genes (DEGs) and differentially expressed long non-coding RNAs (DElncRNAs) was performed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were carried out for DEGs. Then, the construction of the protein-protein interaction (PPI) network was conducted. An lncRNA-miRNA-target ceRNA network was constructed after obtaining microRNAs (miRNA) related to DElncRNAs. Ultimately, key disease-related genes were screened. A total of 399 DEGs and 145 DElncRNAs were obtained. There were 283 nodes and 588 interaction pairs in the PPI network, and synaptosome-associated protein 25 (SNAP25) had higher degrees (degree = 22) in the PPI network. There were 65 interaction pairs in the ceRNA network. Here, Baculoviral IAP Repeat Containing 5 (BIRC5) was regulated by hsa-miR-1285-3p, which was regulated by lncRNA NPHP3-AS1. Gap Junction Protein Alpha 5 (GAJ5) was regulated by hsa-miR-4505, hsa-miR-1972, and hsa-miR-1199-5p. In particular, GAJ5 was enriched in the function of ion transmembrane transport regulation, whereas BIRC5 was enriched in the function of apoptosis-multiple species pathway. Similarly, Potassium Inwardly Rectifying Channel Subfamily J Member 6 (KCNJ6) was enriched in the function of an ion channel complex. VENN analysis identified BIRC5 and GJA5 as key AF-related genes. KCNJ6, SNAP25, GJA5, BIRC5, hsa-miR-1285-3p, and lncRNA NPHP3-AS1 were likely to be associated with AF-VHD development.

N6-methyladenosine (m

Testis-specific protein Y-encoded-like 5 (TSPYL5), a member of the nucleosome assembly protein (NAP) superfamily, functions as a tumor suppressor in ovarian and lung cancer, yet its clinical significance and molecular mechanism in colorectal cancer (CRC) remain unclear. TSPYL5 expression was analyzed using the Gene Expression Profiling Interactive Analysis (GEPIA) database. CRC cell lines HCT116 and HT29 were forced to overexpress TSPYL5 by transfection with pcDNA3.1-TSPYL5. Cell proliferation, apoptosis, migration, and invasion were examined by EdU proliferation assays, flow cytometry, and Transwell assays, respectively. Endoplasmic reticulum stress (ERS) was examined by transmission electron microscopy. Western blot analyses were performed to assess the expression of ERS-associated proteins. GEPIA database analysis showed that CRC patients had lower levels of TSPYL5 expression in their tumor tissues when compared with their para-carcinoma tissues. In vitro experiments indicated that TSPYL5 overexpression significantly suppressed cell proliferation, migration, and invasion, and induced apoptosis and ERS in HCT116 and HT29 cells. Furthermore, the levels of caspase-1, caspase-3, Bax, ATF4, and CHOP protein expression were upregulated after TSPYL5 was overexpressed. In conclusion, our data suggest that TSPYL5 can activate an ERS response that suppresses the proliferation, migration, and invasion of tumor cells. This mechanism may represent a promising therapeutic strategy for CRC.

Integration of HBV DNA into host chromosomes was found in most of the patients with chronic hepatitis B (CHB). In this study, using inverse nested PCR (invPCR), we found the integration site chrX: 111,009,033, which inserted into the p21-activated kinase 3 (PAK3) gene in HepG2.2.15 cells. The viral-human chimeric transcripts were also observed and, significant differences of the copy numbers of integration site chrX: 111,009,033 (P = 0.012) and intra-cell HBV DNA levels (P = 0.027) were found between the cells with and without H2O2 treatment, respectively. This study may provide a novel insight into the elucidation of etiology of HBV integration.

Fluorescence imaging the interplay between lipid droplets (LDs) and protein aggregates (PAs) is extremely valuable for elucidating molecular mechanisms of aging. Here, we describe the first dual-functional fluorescent probe

BackgroundCirculating tumor DNA (ctDNA) levels and blood tumor mutation burden (bTMB) have a significant impact on the prognosis of tumor patients. However, their prognostic role in immune checkpoint inhibitors (ICIs) in cancer patients is still unclear.MethodsWe used the Review Manager software (version 5.3) to perform a meta-analysis based on the published literature to explore the prognostic value of ctDNA and bTMB in patients receiving immunotherapy. We extracted the hazard ratios (HRs) of progression-free survival (PFS) and overall survival (OS) for each included study and their respective 95% confidence intervals (CIs) and p-values for analysis.ResultsThirteen studies were included in the meta-analysis. Higher ctDNA levels were significantly associated with shorter OS (HR = 3.35, 95%CI = 2.49–4.51, p < 0.00001) and PFS (HR = 3.28, 95%CI = 2.47–4.35, p < 0.00001). The results of ctDNA subgroup analysis showed that high posttreatment ctDNA levels significantly correlated with shorter OS in cancer patients receiving ICIs (HR = 5.09, 95%CI = 1.43–18.07, p = 0.01). Moreover, patients with ctDNA clearance had better OS (HR = 4.94, 95%CI = 2.96–8.26, p < 0.00001). Patients with high posttreatment ctDNA levels had shorter PFS (HR = 3.00, 95%CI = 2.02–4.46, p < 0.00001) and those with ctDNA clearance had longer PFS (HR = 4.61, 95%CI = 2.78–7.65, p < 0.00001). However, there was no statistically significant difference in the OS benefits between a high and a low bTMB after ICI therapy (HR = 0.68, 95%CI = 0.33–1.37, p = 0.28).ConclusionsThe host immune system and tumor burden together determine whether cancer patients can benefit from ICI therapy. Our systematic review and meta-analysis revealed for the first time that the levels of pretreatment and posttreatment ctDNA and the clearance of ctDNA can independently be used as prognostic factors for antitumor immunotherapy, while bTMB cannot. In conclusion, ctDNA levels have great potential as an assistant tool for radiological assessments to make clinical therapeutic decisions. The prognostic utility of bTMB still requires further exploration.

Background: Some studies have found that immune cell spatial structures, also known as tertiary lymphoid structures (TLSs), may have prognostic value in cancer. However, these studies all had insufficient sample sizes and inconsistent conclusions. To overcome these problems and draw conclusions, a systematic review and meta-analysis was conducted to determine the prognostic impact of TLSs in cancer.Methods: PubMed, Web of Science, and Embase databases were searched to identify eligible articles published before April 2021. Two authors assessed each record, ensuring that the PICOS framework was met, and risk of bias on the study level was assessed using the Cochrane Bias Risk Assessment Tool. We then performed a meta-analysis using Review Manager 5.3 program to obtain a comprehensive estimate of the prognostic role of TLSs. Results: In total, 28 studies were included in our analysis. We found that in comparison to cancer patients with low TLSs levels, those with high TLSs levels had a better overall survival (OS, hazard ratio [HR]: 0.48, 95% confidence interval [CI]: 0.40-0.57, pConclusion: To the best of our knowledge, this study is the first meta-analysis to show the significant prognostic value of TLSs in cancer, to date. Moreover, TLSs are expected to be a potential marker for predicting anti-tumor immune response, which may help clinicians to select patients with cancer who are sensitive to immunotherapy. In addition to their concentration, TLSs components also had strong prognostic value. In particular, the study may provide a novel therapeutic target for patients with cancer and provide a new theoretical basis for future research on tumor immunotherapy.

Various connecting cables in the electric multiple unit (EMU) are one of main radiation sources in the whole vehicle EMC test. So it is of great significance to research the factors affecting the electromagnetic radiation of cables. A comparative test in the lab was designed. The magnetic and electric field emission data was gathered when the shielding layer of the shielded cable was stripped off or not. Through these tests, some valuable conclusions were obtained. Then some suggestions on cable maintenance and construction were put forward.

The radiation emission of rail transit vehicles in operation is usually affected by environmental factors such as weather. In this paper, the radiation emission of the vehicle is tested under sunny and rainy environmental conditions, and the impact of the vehicle's radiation emission under the rainy environment is obtained by comparison. This not only helps to avoid some unfavorable factors in the test process, but also has certain reference significance for the electromagnetic protection of rail transit vehicles.

ObjectiveThe objective of this systematic review and meta-analysis was to determine the prognostic value of memory CD8(+) T cells in cancer patients with immunotherapy.MethodsEMBASE, MEDLINE (PubMed), and Web of Science databases were searched to identify suitabile articles published before March 2021. Risk of bias on the study level was assessed using the Cochrane Bias Risk Assessment Tool. The hazard ratios (HRs) and 95% confidence intervals (CIs) of pooled progression-free survival (PFS) and overall survival (OS) were calculated using RevMan 5.4 to evaluate the prognostic impact of memory CD8(+) T cells.ResultsIn total, nine studies were included in the final analysis. High levels of memory CD8(+) T cells were significantly closely correlated with better progression-free survival (PFS) and overall survival (OS) of cancer patients with immunotherapy (PFS, HR 0.64, 95% CI 0.53–0.78; OS, HR 0.37, 95% CI 0.21–0.65). Memory CD8(+) T cells still have significant prognostic value in cancer patients given immunotherapy alone after excluding of other interfering factors such as chemotherapy, radiotherapy, and targeted therapy (PFS, HR 0.65, 95% CI 0.48–0.89; OS, HR 0.23, 95% CI 0.13–0.42). However, high memory CD8(+) T cells levels did not correspond to a longer PFS or OS in cancer patients with non-immunotherapy (PFS, HR 1.05, 95% CI 0.63–1.73; OS, HR 1.29, 95% CI 0.48–3.48). Thus, memory CD8(+) T cells might be a promising predictor in cancer patients with immunotherapy.ConclusionsThe host’s overall immune status, and not only the tumor itself, should be considered to predict the efficacy of immunotherapy in cancer patients. This study is the first to show the significant prognostic value of memory CD8(+) T cells in immunotherapy of cancer patients through systematic review and meta-analysis. Thus, the detection of memory CD8(+) T cells has a considerable value in clinical practice in cancer patients with immunotherapy. Memory CD8(+) T cells may be promising immunotherapy targets.

The design and performance of a closed cycle repetitively pulsed non-chain DF laser based on self-initiated volume discharge is presented. In order to effectively solve the problem of large volume uniform glow discharge in non-chain pulsed DF laser, a voltage doubling reverse circuit in the Fitch generator was used. The working mechanism and effects of the voltage doubling circuit was analyzed theoretically according to closed loop equation of RLC circuit, and the results were in good agreement with the experiment. The performance of non-chain DF laser operating in both single pulse mode and repetitive mode was researched. A maximum output energy of 3.65 J and a technical efficiency of 3.12% were recorded in single pulse mode. A laser power of 150 W was obtained at the repetition rate of 50 Hz, the amplitude difference of laser pulse was better than + 8%, and the laser pulse width was about 135 ns.

The aim of this study was to explore the pivotal genes or lncRNAs involved in the progression of atrial fibrillation (AF) -valvular heart disease (VHD). The mRNA profiling GSE113013 was obtained from the Gene Expression Omnibus database. The identification of differentially expressed genes (DEGs) and differentially expressed long non-coding RNAs (DElncRNAs) was performed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were carried out for DEGs. Then, the construction of the protein-protein interaction (PPI) network was conducted. An lncRNA-miRNA-target ceRNA network was constructed after obtaining microRNAs (miRNA) related to DElncRNAs. Ultimately, key disease-related genes were screened. A total of 399 DEGs and 145 DElncRNAs were obtained. There were 283 nodes and 588 interaction pairs in the PPI network, and synaptosome-associated protein 25 (SNAP25) had higher degrees (degree = 22) in the PPI network. There were 65 interaction pairs in the ceRNA network. Here, Baculoviral IAP Repeat Containing 5 (BIRC5) was regulated by hsa-miR-1285-3p, which was regulated by lncRNA NPHP3-AS1. Gap Junction Protein Alpha 5 (GAJ5) was regulated by hsa-miR-4505, hsa-miR-1972, and hsa-miR-1199-5p. In particular, GAJ5 was enriched in the function of ion transmembrane transport regulation, whereas BIRC5 was enriched in the function of apoptosis-multiple species pathway. Similarly, Potassium Inwardly Rectifying Channel Subfamily J Member 6 (KCNJ6) was enriched in the function of an ion channel complex. VENN analysis identified BIRC5 and GJA5 as key AF-related genes. KCNJ6, SNAP25, GJA5, BIRC5, hsa-miR-1285-3p, and lncRNA NPHP3-AS1 were likely to be associated with AF-VHD development.

The present work aimed to explore the aberrant expression of APEX1 in endometrial stromal cells (ESC) and the underlying mechanisms. The levels of APEX1 and miR-24 in endometriosis tissues were tested by qRT-PCR and Western blot. After cell transfection, cells were correspondingly classified into pcDNA3.1-NC, sh-NC, mimic NC, inhibitor NC, pcDNA3.1-APEX1, sh-APEX1, miR-24 mimic, miR-24 inhibitor, sh-NC + inhibitor NC, inhibitor-NC + sh-APEX1, sh-NC + miR-24 inhibitor, pcDNA3.1-NC + mimic NC, mimic NC + pcDNA3.1-APEX1 and pcDNA3.1-NC + miR-24 mimic group. Besides, cell proliferation, apoptosis in addition to apoptosis-related proteins Bax, Bcl-2 and cleaved-casase-3 were analyzed by BrdU assay, flow cytometry (FCM) and Western blot assays, respectively. Additionally, RIP assay was conducted to determine the interaction between pri-miR-24 and miR-24. APEX1 and miR-24 were highly expressed in endometriosis tissues. Overexpression of APEX1 and miR-24 potentiates ESC proliferation and inhibits apoptosis, while those effects could be reversed by APEX1 and miR-24 silencing. Meanwhile, APEX1 and miR-24 could elevate ESC apoptosis-related proteins Bax and cleaved-caspase-3 and decrease Bcl-2 expression. Importantly, APEX1 was positively correlated with miR-24 expression. APEX1 promotes ESC proliferation and inhibits apoptosis by upregulating miR-24 expression.

Background: Abdominal aortic aneurysm (AAA) is a severely life-threating disease; it is generally asymptomatic with diagnosis at a very late stage. Moreover, the genetic components and underpinnings in AAA is considerable with an estimated heritability of up to 70% roughly. Therefore, identifying the biomarkers for AAA is valuable toward predicting and eventually inspecting the high-risk populations. Methods: Herein, we used integrative bioinformatics and experimental analyses to reveal that protein tyrosine phosphatase non-receptor type 22 (PTPN22) can be a potentially diagnostic biomarker for AAA.Results: PTPN22, whose expression is significantly upregulated in aortic tissues of AAA, was primarily identified as a genetic biomarker for AAA, while it has no diagnostic value for patients with thoracic aortic aneurysm. Moreover, CaCl2-based in vitro AAA model was adopted to experimentally show that Garcinia acid could be a therapeutic drug for AAA. Conclusions: Collectively, these results indicate that PTPN22 may be a potentially diagnostic biomarker for AAA.

Due to the impact of COVID-19 pandemic, there is a high demand for hospitals all over the world, and hospitals also bear huge medical pressure and responsibility. Medical research is the driving force to promote the continuous development of medicine, and how to do a good job in the development of medical research informatization is one of the keys. This study from China’s large public hospitals as the research object, pointed out the shortcomings and problems of hospital scientific research management, put forward that the development of information technology is the relatively backward solution of scientific research management in hospitals, and then put forward the development strategy of medical research informatization based on the current situation.

To detect the expressions of CD74 and matrix metalloproteinase-9 (MMP-9) in colon adenocarcinomas, and to explore the relationship between the expressions and clinicopathological characteristics and prognosis.98 cases of colon adenocarcinoma tissues from patients who underwent colon cancer resection in the Sixth Affiliated Hospital, Sun Yat-sen University from January 2013 to March 2015 comprised the experimental group, while 71 cases of colon mucosa tissues from patients who underwent colon polypectomy during the same period comprised the control group. qRT-PCR was used to detect the expressions of CD44 and MMP-9 mRNAs in the two groups, in order to analyze their correlation in colon adenocarcinomas, and to also analyze their relationship with clinicopathological characteristics and prognosis.The expressions of CD74 and MMP-9 mRNAs in colon adenocarcinoma tissues were significantly higher than those in normal colon mucosa tissues (p0.05). The expressions of CD74 and MMP-9 mRNAs had no significant relationship with the patient's gender, age, differentiation grade and tumor type in colon adenocarcinoma tissues (p0.05), but had significant correlation with lymph node metastasis and pathological stage (p0.05). According to the average expressions of CD74 and MMP-9 mRNAs, the patients were divided into low and high expression groups. The 3-year survival rate of patients in the low expression group was significantly higher than that in the high expression group (p0.05). Moreover, the expressions of CD74 and MMP-9 were positively correlated (r = 0.853, p0.001).CD74 and MMP-9 are highly expressed in colon adenocarcinomas, and their expressions are closely related to the pathological stage, lymph node metastasis and prognosis of colon adenocarcinoma patients. Therefore, they can be used as important biological markers for diagnosis and prognosis prediction of colon adenocarcinoma.