Search

Distant metastasis frequently occurs in oral squamous cell carcinoma (OSCC) and contributes to the adverse prognosis for patients with OSCC. However, the potential mechanisms behind the metastasis have not yet been clarified. This study investigated the role of miR-378 in the migration and invasiveness of OSCC in vitro and in vivo. According to our results, the migration and invasiveness of OSCC cells were increased in cells overexpressing miR-378, and reduced in cells where miR-378-3p/5p was silenced. In addition, overexpression of miR-378 suppressed the expressions and activities of matrix metalloproteinase 9 (MMP-9) and MMP-2. Epithelial--mesenchymal transition (EMT) was restrained by overexpression of miR-378, as evidenced by an increase in E-cadherin expression and decrease in N-cadherin and uPA expression. However, knockdown of miR-378-3p/5p produced the opposite results. Moreover, kallikrein-related peptidase 4 (KLK4) was confirmed to be a target gene of miR-378. Overexpression of KLK4 reversed the induced decrease in migration and invasiveness of cells overexpressing miR-378 by upregulating the levels of MMP-9, MMP-2, and N-cadherin, and downregulating the level of E-cadhrin. Finally, the number of metastasis nodules in the lung tissues of nude mice was reduced by overexpression of miR-378, whereas the number of metastases increased with knockdown of miR-378. Taken together, our results suggest that the miR-378--KLK4 axis is involved in the mechanisms behind the migration and invasiveness of OSCC cells. Targeting the miR-378--KLK4 axis may be an effective measure for treating OSCC. Key words: miR-378, oral squamous cell carcinoma, KLK4, migration, invasiveness. La métastase à distance survient fréquemment dans le carcinome épidermoïde oral (CEO) et elle contribue au mauvais pronostic du CEO. Toutefois, les mécanismes potentiels qui sous-tendent la métastase n'ont pas encore été clarifiés. Cette étude visait à évaluer le rôle du miR-378 dans la migration et l'invasion du CEO in vitro et in vivo. Selon les résultats obtenus par les auteurs, les capacités de migration et d'invasion étaient accrues chez les cellules qui surexprimaient le miR-378, alors qu'elles diminuaient chez les cellules soumises à un silençage du miR-378-3p/5p. De plus, la surexpression du miR-378 supprimait l'expression et l'activité de MMP-9 et MMP-2. La transition épithélio-mésenchymateuse (TEM) était freinée par la surexpression du miR-378 comme le démontrait l'accroissement de l'expression de la E-cadhérine et la diminution de l'expression de la N-cadhérine et de l'urokinase. En outre, le knockdown du miR-378-3p/5p exerçait l'effet inverse. De plus, la peptidase KLK4 (kallikrein-related peptidase 4) s'avérait être le gène cible du miR-378. La surexpression de KLK4 renversait la diminution de la migration et de l'invasion des cellules induites par la surexpression de miR-378, au moyen de la régulation à la hausse des niveaux de MMP-9, MMP-2 et de N-cadhérine et la régulation à la baisse des niveaux de E-cadhérine. Finalement, le nombre de métastases pulmonaires chez les souris nues était réduit par la surexpression du miR-378 alors que le knockdown du miR-378 provoquait une augmentation du nombre de nodules métastatiques. Dans l'ensemble, leur étude suggère que l'axe miR-378-KLK4estimpliqué dans les mécanismesdemigrationetd'invasion des cellulesdeCEO.Leciblagedel'axe miR-378--KLK4 pourrait constituer une mesure efficace de traitement du CEO. [Traduit par la Rédaction] Mots-clés : miR-378, carcinome épidermoïde oral, KLK4, migration, invasion., Oral malignancy is one of the most common kinds of head and neck cancer, and its main pathology type is squamous-cell carcinoma. The overall prognosis for patients with oral squamous [...]

A porous amino resin melamine–formaldehyde (MF) with a high content of hydroxymethyl groups was prepared via the polycondensation of an excess of formaldehyde with melamine. Polyethylene imine (PEI) was then grafted onto the porous amino resin via a Mannich reaction between the active hydroxymethyl groups on the MF resin and the amino group on PEI to synthesize a solid amine adsorbent (MF-g-PEI). A large amount of hydroxymethyl groups were generated on the synthesized amino resin due to the excess formaldehyde used in the polycondensation reaction, which will provide abundant active sites to firmly combine with PEI and thus, enhance the stability of the MF-g-PEI adsorbent. The BET surface area and the pore volume of MF-g-PEI prepared using the optimal conditions were 460 m2 g−1 and 0.76 cm3 g−1, respectively. The CO2 adsorption capacity of MF-g-PEI, with a PEI loading of 11.35% can reach 1.32 mmol g−1 at 10 °C, while its amine utilization efficiency can reach up to 48.9%. The adsorbents also exhibited significantly improved adsorption kinetics and regeneration performance. This work provides a novel method that can achieve a promising solid amine adsorbent by directly grafting PEI onto porous material in the absence of an intermediate. [ABSTRACT FROM AUTHOR]