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Triploid hybrid (3n = 150) of red crucian carp (♀, 2n = 100) and allotetraploid (♂, 4n = 200) display improved disease resistance and stress resistance than their parents. In order to elucidate their innate immune mechanisms, three novel cell lines from the caudal fin of red crucian carp, triploid hybrid and allotetraploid (named 2nFC, 3nFC and 4nFC accordingly) were established and characterized respectively. 2nFC, 3nFC and 4nFC showed fibroblast-like morphology and characteristics. They have been subcultured for more than 100 passages since the initial primary culture. Viral infection experiments showed that 2nFC, 3nFC and 4nFC were susceptible to spring viraemia of carp virus (SVCV) infection. Intriguingly, 3nFC performed the stronger resistance ability against SVCV than 2nFC and 4nFC, which indicated that 2nFC, 3nFC and 4nFC might be used as the suitable in vitro models for exploring and analyzing the differences among these three cyprinid fishes in antiviral innate immune mechanisms. Based on this, we analyzed the transcriptome profile of 2nFC, 3nFC and 4nFC in the context of SVCV infection. The KEGG enrichment analysis showed that the differentially expressed genes (DEGs) were primarily enriched to immune-related signaling pathways. However, some signaling pathways against viral infection were activated remarkably in 2nFC and 3nFC but not in 4nFC. Overall, the establishment of 2nFC, 3nFC and 4nFC provided us a suitable platform to elucidate the innate immunity of fishes with different ploidy and clear genetic relationship. [ABSTRACT FROM AUTHOR]

Background: The efficacy of a regimen combining Tegafur, Gimeracil and Oteracil Potassium Capsules (S-1), oxaliplatin (SOX) with trastuzumab and tislelizumab chemotherapy for advanced gastric cancer (GC) has not been reported., Case Summary: A 56-year-old male was diagnosed with GC combined with peripheral lymph node metastasis. The patient received neoadjuvant chemotherapy, including SOX, tislelizumab and trastuzumab. After 4 cycles of chemotherapy, the tumor shrank significantly, and radical surgery was performed with good clinical results. To date, the patient has been followed up for 6 months with no significant side effects., Conclusion: In this study, the patient received combination chemotherapy with SOX trastuzumab and tislelizumab and successfully underwent radical surgery with good clinical outcomes. Combined SOX with trastuzumab and tislelizumab may be an effective neoadjuvant chemotherapy regimen., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhu, Da, Li and Peng.)

Amount of studies in cells and animal models have proved vitamin D has multifarious antitumor effects. However, epidemiological studies showed inconsistent result on gastric cancer. The antitumor role is mainly mediated by the vitamin D receptor (VDR). Our hypothesis is that VDR may be abnormally (poorly) expressed in gastric cancer tissue. Present study is aimed at discovering and analyzing VDR expression in a series of human gastric tissues, including normal, premalignant, and malignant gastric tissue, and correlated VDR to the clinicopathological parameters of gastric cancer patients. VDR expression was detected by immunohistochemistry. The χ(2) test was used to analyze the VDR expression as well as the relationship between VDR and the clinicopathological factors of gastric cancer patients. Compared with normal (82.61%) and premalignant tissues (73.64%), VDR was lower expressed in cancer tissues (57.61%), with a statistically significant difference (P = 0.001). Among cancer tissues, VDR was higher expressed in well and moderate differentiated tissues contrasted with tissues with poor differentiation, and higher expressed in small tumors (< 5 cm) compared with large tumors (≥ 5 cm), with a statistically significant difference respectively (P = 0.016, P = 0.009). A decline linear trend appeared when analyzing the statistical difference of VDR expression among normal, premalignant, and malignant gastric tissues. VDR expression has been on the decline from the premalignant stage, finally low expressed in gastric cancer tissues, especial in poorly differentiated tissues. VDR could be a potential prognostic factor for patients with gastric cancer.

Multiple primary malignant neoplasms (MPMN) are two or more malignancies in an individual without any relationship between the tumors. In recent years, increasing number of cases were reported. However, Synchronous double primary gastric and endometrial cancer are relatively rare to be reported. Here we present a rare case of synchronous double cancer involving the stomach and endometrium, which is resected simultaneously and received six times chemo. After reviewing lots of literatures at home and abroad, we discuss the risk factors, the diagnostic criteria, the treatment modalities and the prognosis of these rare MPMN. Although a number of risk factors have been proposed in a mass of literatures, but the mechanism of MPMN is still unclear. We didn't discover a detailed explanation for the mechanism of MPMN from our patient. Therefore, further research should focus on the etiology and mechanism of MPMN.