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Your search keyword '"Pendexter, Casie A."' showing total 32 results
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32 results on '"Pendexter, Casie A."'

2. Partial freezing of rat livers extends preservation time by 5-fold

Author

Tessier, Shannon N., de Vries, Reinier J., Pendexter, Casie A., Cronin, Stephanie E. J., Ozer, Sinan, Hafiz, Ehab O. A., Raigani, Siavash, Oliveira-Costa, Joao Paulo, Wilks, Benjamin T., Lopera Higuita, Manuela, van Gulik, Thomas M., Usta, Osman Berk, Stott, Shannon L., Yeh, Heidi, Yarmush, Martin L., Uygun, Korkut, and Toner, Mehmet

Published
2022
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4. Machine Perfusion Enables 24-h Preservation of Vascularized Composite Allografts in a Swine Model of Allotransplantation.

Author

Goutard, Marion, Tawa, Pierre, Berkane, Yanis, Andrews, Alec R., Pendexter, Casie A., de Vries, Reinier J., Pozzo, Victor, Romano, Golda, Lancia, Hyshem H., von Reiterdank, Irina Filz, Bertheuil, Nicolas, Rosales, Ivy A., How, Ira Doressa Anne L., Randolph, Mark A., Lellouch, Alexandre G., Cetrulo Jr., Curtis L., and Uygun, Korkut

Subjects
PRESERVATION of organs, tissues, etc., HOMOGRAFTS, PERFUSION, SWINE, COLD storage
Abstract

The current gold standard for preserving vascularized composite allografts (VCA) is 4°C static cold storage (SCS), albeit muscle vulnerability to ischemia can be described as early as after 2 h of SCS. Alternatively, machine perfusion (MP) is growing in theworld of organ preservation. Herein, we investigated the outcomes of oxygenated acellular subnormothermic machine perfusion (SNMP) for 24-h VCA preservation before allotransplantation in a swine model. Six partial hindlimbs were procured on adult pigs and preserved ex vivo for 24 h with either SNMP (n = 3) or SCS (n = 3) before heterotopic allotransplantation. Recipient animals received immunosuppression and were followed up for 14 days. Clinical monitoring was carried out twice daily, and graft biopsies and blood samples were regularly collected. Two blinded pathologists assessed skin and muscle samples. Overall survival was higher in the SNMP group. Early euthanasia of 2 animals in the SCS group was linked to significant graft degeneration. Analyses of the grafts showed massive muscle degeneration in the SCS group and a normal aspect in the SNMP group 2 weeks after allotransplantation. Therefore, this 24-h SNMP protocol using a modified Steen solution generated better clinical and histological outcomes in allotransplantation when compared to time-matched SCS. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Sub-Zero Non-Freezing of Vascularized Composite Allografts Preservation in Rodents

Author

von Reiterdank, Irina Filz, primary, Tawa, Pierre, additional, Berkane, Yanis, additional, de Clermont-Tonnerre, Eloi, additional, Dinicu, Antonia, additional, Pendexter, Casie, additional, Goutard, Marion, additional, Lellouch, Alexandre G., additional, Molen, Aebele B. Mink van der, additional, Coert, J. Henk, additional, Cetrulo, Curtis L., additional, and Uygun, Korkut, additional

Published
2023
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8. Zebrafish as a high throughput model for organ preservation and transplantation research

Author

Da Silveira Cavalcante, Luciana, primary, Lopera Higuita, Manuela, additional, González‐Rosa, Juan Manuel, additional, Marques, Beatriz, additional, To, Samantha, additional, Pendexter, Casie A., additional, Cronin, Stephanie E. J., additional, Gopinathan, Kaustav, additional, de Vries, Reinier J., additional, Ellett, Felix, additional, Uygun, Korkut, additional, Langenau, David M., additional, Toner, Mehmet, additional, and Tessier, Shannon N., additional

Published
2023
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11. Development of a rat forelimb vascularized composite allograft (VCA) perfusion protocol

Author

Pendexter, Casie A., primary, Haque, Omar, additional, Mojoudi, Mohammadreza, additional, Maggipinto, Sarah, additional, Goutard, Marion, additional, Baicu, Simona, additional, Lellouch, Alexandre G., additional, Markmann, James F., additional, Brandacher, Gerald, additional, Yeh, Heidi, additional, Tessier, Shannon N., additional, Cetrulo, Curtis, additional, and Uygun, Korkut, additional

Published
2023
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12. The role of antifreeze glycoprotein (AFGP) and polyvinyl alcohol/polyglycerol (X/Z-1000) as ice modulators during partial freezing of rat livers

Author

Tessier, Shannon N., primary, Haque, Omar, additional, Pendexter, Casie A., additional, Cronin, Stephanie E. J., additional, Hafiz, Ehab O. A., additional, Weng, Lindong, additional, Yeh, Heidi, additional, Markmann, James F., additional, Taylor, Michael J., additional, Fahy, Gregory M., additional, Toner, Mehmet, additional, and Uygun, Korkut, additional

Published
2022
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14. Tools for heart preservation research

Author

Higuita, Manuela Lopera, primary, Da Silveira Cavalcante, Luciana, additional, González Rosa, Juan Manuel, additional, Pendexter, Casie, additional, Gopinathan, Kaustav, additional, To, Samantha, additional, De Vries, Reinier, additional, Ellett, Felix, additional, Uygun, Korkut, additional, Langenau, David M., additional, Toner, Mehmet, additional, and Tessier, Shannon N., additional

Published
2022
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15. Exceeding the Limits of Static Cold Storage in Limb Transplantation Using Subnormothermic Machine Perfusion.

Author

Goutard, Marion, de Vries, Reinier J., Tawa, Pierre, Pendexter, Casie A., Rosales, Ivy A., Tessier, Shannon N., Burlage, Laura C., Lantieri, Laurent, Randolph, Mark A., Lellouch, Alexandre G., Cetrulo Jr, Curtis L., and Uygun, Korkut

Subjects
COLD storage, PERFUSION, PRESERVATION of organs, tissues, etc., LABORATORY rats, VASCULAR resistance, KIDNEY transplantation
Abstract

Background For 50 years, static cold storage (SCS) has been the gold standard for solid organ preservation in transplantation. Although logistically convenient, this preservation method presents important constraints in terms of duration and cold ischemia-induced lesions. We aimed to develop a machine perfusion (MP) protocol for recovery of vascularized composite allografts (VCA) after static cold preservation and determine its effects in a rat limb transplantation model. Methods Partial hindlimbs were procured from Lewis rats and subjected to SCS in Histidine-Tryptophan-Ketoglutarate solution for 0, 12, 18, 24, and 48 hours. They were then either transplanted (Txp), subjected to subnormothermic machine perfusion (SNMP) for 3 hours with a modified Steen solution, or to SNMP + Txp. Perfusion parameters were assessed for blood gas and electrolytes measurement, and flow rate and arterial pressures were monitored continuously. Histology was assessed at the end of perfusion. For select SCS durations, graft survival and clinical outcomes after transplantation were compared between groups at 21 days. Results Transplantation of limbs preserved for 0, 12, 18, and 24-hour SCS resulted in similar survival rates at postoperative day 21. Grafts cold-stored for 48 hours presented delayed graft failure (p = 0.0032). SNMP of limbs after 12-hour SCS recovered the vascular resistance, potassium, and lactate levels to values similar to limbs that were not subjected to SCS. However, 18-hour SCS grafts developed significant edema during SNMP recovery. Transplantation of grafts that had undergone a mixed preservation method (12-hour SCS + SNMP + Txp) resulted in better clinical outcomes based on skin clinical scores at day 21 post-transplantation when compared to the SCS + Txp group (p = 0.01613). Conclusion To date, VCA MP is still limited to animal models and no protocols are yet developed for graft recovery. Our study suggests that ex vivo SNMP could help increase the preservation duration and limit cold ischemia-induced injury in VCA transplantation. [ABSTRACT FROM AUTHOR]

Published
2023
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16. Exceeding the Limits of Static Cold Storage in Limb Transplantation Using Subnormothermic Machine Perfusion

Author

Goutard, Marion, additional, de Vries, Reinier J., additional, Tawa, Pierre, additional, Pendexter, Casie A., additional, Rosales, Ivy A., additional, Tessier, Shannon N., additional, Burlage, Laura C., additional, Lantieri, Laurent, additional, Randolph, Mark A., additional, Lellouch, Alexandre G., additional, Cetrulo Jr, Curtis L., additional, and Uygun, Korkut, additional

Published
2022
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18. Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts

Author

Burlage, Laura C., primary, Lellouch, Alexandre G., additional, Taveau, Corentin B., additional, Tratnig-Frankl, Philipp, additional, Pendexter, Casie A., additional, Randolph, Mark A., additional, Porte, Robert J., additional, Lantieri, Laurent A., additional, Tessier, Shannon N., additional, Cetrulo, Curtis L., additional, and Uygun, Korkut, additional

Published
2022
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19. Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion

Author

de Vries, Reinier J., primary, Cronin, Stephanie E. J., additional, Romfh, Padraic, additional, Pendexter, Casie A., additional, Jain, Rohil, additional, Wilks, Benjamin T., additional, Raigani, Siavash, additional, van Gulik, Thomas M., additional, Chen, Peili, additional, Yeh, Heidi, additional, Uygun, Korkut, additional, and Tessier, Shannon N., additional

Published
2021
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20. The role of antifreeze glycopeptides (AFGP) and polyvinyl alcohol/polyglycerol (X/Z-1000) cocktails as ice modulators during partial freezing of rat livers

Author

Tessier, Shannon N., primary, Haque, Omar, additional, Pendexter, Casie A., additional, Cronin, Stephanie E.J., additional, Weng, Lindong, additional, Yeh, Heidi, additional, Markmann, James F., additional, Taylor, Michael J., additional, Fahy, Gregory M., additional, Toner, Mehmet, additional, and Uygun, Korkut, additional

Published
2021
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23. Extending preservation duration of hearts and livers with partial freezing

Author

Da Silveira Cavalcante, Luciana, primary, Pendexter, Casie A., additional, Cronin, Stephanie E.J., additional, de Vries, Reinier J., additional, Ellett, Felix, additional, Marques, Beatriz, additional, Gopinathan, Kaustav, additional, González-Rosa, Juan Manuel, additional, Langenau, David M., additional, Uygun, Korkut, additional, Toner, Mehmet, additional, and Tessier, Shannon N., additional

Published
2020
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24. Abstract 403: Leveraging the Zebrafish to Overcome Barriers in Heart Transplantation

Author

Tessier, Shannon N, primary, Da Silveira Cavalcante, Luciana, additional, Pendexter, Casie A, additional, Cronin, Stephanie E, additional, de Vries, Reinier J, additional, Ellett, Felix, additional, Marques, Beatriz, additional, Gopinathan, Kaustav, additional, To, Samantha, additional, Gonzalez-rosa, Juan Manuel, additional, Langenau, David M, additional, Uygun, Korkut, additional, and Toner, Mehmet, additional

Published
2020
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27. Partial Freezing: A New Integrated Approach To Organ Banking

Author

Tessier, Shannon, primary, Cronin, Stephanie, additional, Pendexter, Casie, additional, De Vries, Reinier, additional, Kimura, Shoko, additional, Ozer, Sinan, additional, Yeh, Heidi, additional, Uygun, Korkut, additional, Toner, Mehmet, additional, Banik, Peony, additional, and Nagpal, Sonal, additional

Published
2019
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29. Twenty-four hour ex-vivo normothermic machine perfusion in rat livers.

Author

Haque, Omar, Pendexter, Casie A., Cronin, Stephanie E. J., Raigani, Siavash, de Vries, Reiner J., Yeh, Heidi, Markmann, James F., and Uygun, Korkut

Subjects
RATS, LIVER, OXYGEN consumption, HISTOLOGY, MACHINERY, LIVER diseases, PERFUSION
Abstract

Ex-vivo liver perfusion (EVLP) is an ideal platform to study liver disease, therapeutic interventions, and pharmacokinetic properties of drugs without any patient risk. Rat livers are an ideal model for EVLP due to less organ quality variability, ease of hepatectomy, well-defined molecular pathways, and relatively low costs compared to large animal or human perfusions. However, the major limitation with rat liver normothermic machine perfusion (NMP) is maintaining physiologic liver function on an ex-vivo machine perfusion system. To address this need, our research demonstrates 24-hour EVLP in rats under normothermic conditions. Early (6 hour) perfusate transaminase levels and oxygen consumption of the liver graft are shown to be good markers of perfusion success and correlate with viable 24-hour post-perfusion histology. Finally, we address overcoming challenges in long-term rat liver perfusions such as rising intrahepatic pressures and contamination, and offer future directions necessary to build upon our work. [ABSTRACT FROM AUTHOR]

Published
2020
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32. Modified Langendorff Perfusion Method for Extended Perfusion Times of Rodent Cardiac Grafts.

Author

Pendexter CA, Bolger-Chen M, Lopera Higuita M, Cronin SEJ, Rabi SA, Osho AA, and Tessier SN

Subjects
Animals, Rats, Heart Transplantation methods, Isolated Heart Preparation methods, Perfusion methods
Abstract

Despite important advancements in the diagnosis and treatment of cardiovascular diseases (CVDs), the field is in urgent need of increased research and scientific advancement. As a result, innovation, improvement and/or repurposing of the available research toolset can provide improved testbeds for research advancement. Langendorff perfusion is an extremely valuable research technique for the field of CVD research that can be modified to accommodate a wide array of experimental needs. This tailoring can be achieved by personalizing a large number of perfusion parameters, including perfusion pressure, flow, perfusate, temperature, etc. This protocol demonstrates the versatility of Langendorff perfusion and the feasibility of achieving longer perfusion times (4 h) without graft function loss by utilizing lower perfusion pressures (30-35 mmHg). Achieving extended perfusion times without graft damage and/or function loss caused by the technique itself has the potential to eliminate confounding elements from experimental results. In effect, in scientific circumstances where longer perfusion times are relevant to the experimental needs (i.e., drug treatments, immunological response analysis, gene editing, graft preservation, etc.), lower perfusion pressures can be key for scientific success.

Published
2024
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