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While it has been well evidenced that plant domestication affects the structure of the root-associated microbiome, there is a poor understanding of how domestication‐mediated differences between rhizosphere microorganisms functionally affect microbial ecosystem services. In this study, we explore how domestication influenced functional assembly patterns of bacterial communities in the root‐associated soil of 27 tomato accessions through a transect of evolution, from plant ancestors to landraces to modern cultivars. Based on molecular analysis, functional profiles were predicted and co‐occurrence networks were constructed based on the identification of co‐presences of functional units in the tomato root‐associated microbiome. The results revealed differences in eight metabolic pathway categories and highlighted the influence of the host genotype on the potential functions of soil bacterial communities. In general, wild tomatoes differed from modern cul-tivars and tomato landraces which showed similar values, although all ancestral functional characteristics have been conserved across time. We also found that certain functional groups tended to be more evolutionarily conserved in bacterial communities associated with tomato landraces than those of modern varieties. We hypothesize that the capacity of soil bacteria to provide ecosystem services is affected by agronomic practices linked to the domestication process, particularly those related to the preservation of soil organic matter.

Background: Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay. Results: MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP's mutagenic activity was assessed using the Salmonella typhimurium reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP's lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP. Conclusion: EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.

Suelos. Características petroquímicas y micromorfológicas de Haploxeralfs, por P. Arévalo. J. Gallardo y J. Benayas.-- Determinación de manganeso total en suelos, por J. Moreno Caselles, M. Guillén y M. Romero.-- Rendolles de la Sierra de María (Almería), por L J. Alias y J. Martínez.-- Estudio de tres perfiles de suelos de La Alpujarra, por Barahona. E., García Chicano. J. L. Guardiola. J. L. lriarte. A, Pérez-Pujalte, A. y Quirantes, J.-- Detección de concordancias fisonómico-edáficas por J. M. Gómez. R. Rodríguez González. A. García Miranda y C. de Hoyos Alonso.-- Suelos y sedimentos de zonas semiáridas, por A. García Rodríguez, J. Forteza Bonnin y L F. Lorenzo Martín.-- Suelos sobre jumillita de la Sierra de las Cabras (Albacete), por Joaquín Hernández Bastida, María Teresa Fernández Tapia y Francisco Alcaraz Ariza.-- Estudio microscópico de aridisoles de las Islas Canarias, por A. Rodríguez Rodríguez, M. C. Pérez Angles. M. C. Díez de la Lastra Bosch y E. Fernández Caldas.-- El material original: propiedades de los suelos de Galicia, por F. Macias. R. M. Calvo, C. García. E. García-Rodeja y B. Silva.-- Las sierras de Queixa e invernadeiro y sus estribaciones, por Grupo de Edafólogos de Santiago.-- Suelos forestales de la cordillera costero-catalana, por A. Escuredo. V. R. Vallejo y J. Bech.-- Materia orgánica de suelos forestales, por A. Escuredo. V. Vallejo y J. Bech.--Dificultades en el empleo del sistema "USDA-Soil Taxonomy", por Carlos Roquero de Laburu.-- Nematodos fitoparásitos de la superfamilia "criconematoidea", por A. Bello y Ma. Paz Lara.—Silicatos. Retención del diazinon por montmorillonita, sepiolita y caolinita, por G. Dios Cancela, S. González García y M. Martín Aguilar.—Geoquímica. Alteración de minerales de estaño, por Guijarro, J. Casas, J. y Hoyos, M.A.—Nutrición y Fisiología Vegetal.-- Capacidad fotosintética de maíz, por U. Velázquez, M. Lasaña y J. Cardus.-- El alerce en Chile. II. Su nutrición mineral, por M. Lachica, C. González O. y M.