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Introduction and Objective: The aim of this study was to enhance the understanding of the tensor vastus intermedius (TVI), a recently recognized addition to the quadriceps femoris muscle group, by conducting a systematic literature review with empirical cadaveric research to provide comprehensive data on its anatomical features, including dimensions, topography, variations, and prevalence., Material and Methods: An exhaustive literature review was conducted using medical databases such as PubMed, MEDLINE, and Scopus. This review was complemented by original cadaveric research involving 30 autopsied adult bodies, encompassing 60 lower limbs, to empirically investigate the TVI's anatomical characteristics., Results: The TVI was consistently identified in the medial aspect between the vastus lateralis (VL) and vastus intermedius (VI) in all examined cases. Detailed documentation covered its anatomical specifics, e.g. location, origin, and fascial connections, categorized using the criteria of Grob et al. Measurements of the muscle belly - length, width, and thickness - showed no significant lateral differences, but revealed gender-based disparities in TVI's size., Conclusions: The review not only confirms the consistent presence of the TVI, but also addresses the discrepancies in existing literature about its anatomical distribution and dimensional metrics. The observed methodological variations in previous studies underscore the need for standardized research methodologies in muscle anatomy. This in-depth review analysis significantly contributes to a deeper understanding of TVI, proving particularly beneficial for professionals in rehabilitation and related medical fields.

Background: The stage of colorectal cancer (CRC) at the day of diagnosis has the greatest influence on survival rate. Thus, for CRC, which is mainly identified as advanced disease, non-invasive, molecular blood or stool tests could boost the diagnosis and lower mortality. Evaluation of miRNA expression levels in serum of patients diagnosed with CRC is a potential tool in early screening. Screening can be supported by machine learning (ML) as a tool for developing a cancer risk predictive model based on genetic data., Materials and Methods: miRNA was isolated from the serum of 8 patients diagnosed with CRC and 10 patients from a control group matched for age and sex. The expression of 179 miRNAs was determined using a serum/plasma panel (Exiqon). Determinations were conducted using real-time PCR technique on an Applied Biosystems QuantStudio3 device in 96-well plates. A predictive model was developed through the Azure Machine Learning platform., Results: A wide panel of 29 up-regulated miRNAs in CRC were identified and divided into two subgroups: 1) miRNAs with significantly higher serum level in cancer patients vs. controls (24 miRNAs) and 2) miRNAs detected only in cancer patients and not in controls (5 miRNAs). Re-analysis of published miRNA profiles of CRC tumours or CRC exosomes revealed that only 2 out of 29 miRNAs were up-regulated in all datasets including ours (miR-34a and miR-25-3p)., Conclusion: Our research suggests the potential role of overexpressed miRNAs as diagnostic or prognostic biomarkers among CRC patients. Such clustering of miRNAs may be a potential direction for discovering new diagnostic panels of cancer (including CRC), especially using ML. The low correspondence between deregulation of miRNAs in serum and tumour tissue revealed in our study confirms previously published reports., (Copyright © 2022, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)

Kynurenic acid (KYNA) is a metabolite of tryptophan formed enzymatically along kynurenine pathway in bacteria, fungi, plants and animals. It was suggested that yeast may produce KYNA during the fermentation process. Since KYNA was found to interact with alcohol metabolism by inhibition of aldehyde dehydrogenase activity the aim of this study was to measure the content of KYNA in selected alcoholic beverages of various type, beer, wine, mead and spirits. Moreover, the absorption and elimination rate of KYNA administered as a beverage was investigated in humans. Twelve healthy volunteers (6 female and 6 male) were studied. Fifty six samples of alcoholic beverages were of commercial origin. KYNA was determined by means of high-performance liquid chromatography method with fluorometric detection. KYNA was identified in all studied beverages. The amounts of KYNA found in various types of beverages differed significantly: mead 9.4-38.1 µg/100 ml, wine 1.4-10.9 µg/100 ml, beer 0.1-5.2 µg/100 ml, spirits 0.01-0.1 µg/100 ml. In human, it was found that KYNA is rapidly absorbed from digestive tract reaching its maximal concentration in blood 30 min after administration. Thus, the potential interaction between KYNA and alcohol occurring in human body after ingestion of alcoholic beverages was proven., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Background: Kynurenic acid (KYNA) is a biologically active metabolite of tryptophan exerting action on several receptors located in the brain and periphery. KYNA can be synthesized endogenously or supplied in the diet. It was documented that KYNA is present in various types of food. However, its presence in beverages was not yet investigated. Here, we measured content of KYNA in tea and coffee as well as analyzed distribution and fate of intragastrically administered labelled KYNA in mice., Methods: 16 and 13 studied samples of tea and coffee, respectively were of commercial origin. Tea and coffee infusions were prepared according to the producers' guidelines. KYNA content in beverages was measured by means of HPLC detection. Adult male mice were used for analysis of fate of intragastrically administered labelled KYNA and collected samples were analyzed using liquid scintillation counter., Results: KYNA was identified in all studied beverages. Amounts of KYNA found in various types of beverages differed significantly. The highest content of KYNA in tea and coffee was 8.7 μg/100 ml and 0.63 μg/100 ml, respectively. It was found that KYNA administered intragastrically as a liquid is absorbed from the digestive system and readily excreted in urine. The atypical kinetics of KYNA distribution were found in intestinal content of cecum, where it appeared later and persisted longer than in other tissues., Conclusions: Our data show that tea and coffee intake may contribute to KYNA content in the human organism. The distribution pattern of KYNA delivered as a liquid suggests that it either directly affects digestive system's functioning and intestinal microbiome composition, or participates in the whole body pool of KYNA., (Copyright © 2018 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.)

Background: Virtual colonoscopy (VC) enables three-dimensional view of walls and internal lumen of the colon as a result of reconstruction of multislice CT images. The role of VC in diagnosis of the colon abnormalities systematically increases, and in many medical centers all over the world is carried out as a screening test of patients with high risk of colorectal cancer., Material/methods: We analyzed results of virtual colonoscopy of 360 patients with clinical suspicion of colorectal cancer. Sensitivity and specificity of CT colonoscopy for detection of colon cancers and polyps were assessed., Results: Results of our research have shown high diagnostic efficiency of CT colonoscopy in detection of focal lesions in large intestine of 10 mm or more diameter. Sensitivity was 85.7%, specificity 89.2%., Conclusions: Virtual colonoscopy is noninvasive and well tolerated by patients imaging method, which permits for early detection of the large intestine lesions with specificity and sensitivity similar to classical colonoscopy in screening exams in patients suspected for colorectal cancer. Good preparation of the patients for the examination is very important for proper diagnosis and interpretation of this imaginge procedure.