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The purpose of the current study was to find out whether escin (ES) safeguarded experimental rats against cyclophosphamide (CP)-induced kidney injury. Twenty-four rats were randomly divided into four groups ( n = 6). After the examination, histological and biochemical analyses were performed to assess the alterations in kidney tissue. According to histologic and biochemical analyses, renal tissue in the CP group suffered significant damage by CP. There was a significant improvement in histological damage in the group receiving CP+ES together. This suggests that ES significantly protects the kidney's functional characteristics. The present study concludes by highlighting histological and biochemical studies to illustrate the ability of ES to cure kidney injury caused by CP and its influence on the relationship between oxidative stress, apoptosis, and renal failure.

Cyclophosphamide (CTX) is the most commonly used effective alkylating drug in cancer treatment, but its use is restricted because its toxic side effect causes testicular toxicity. CTX disrupts the tissue redox and antioxidant balance and the resulting tissue damage causes oxidative stress. In our study based on this problem, kefir against CTX-induced oxidative stress and testicular toxicity were investigated. Rats were divided into 6 groups: control, 150 mg/kg CTX, 5 and 10 mg/kg kefir, 5 and 10 mg/kg kefir + 150 CTX. While the fermented kefirs were mixed and given to the rats for 12 days, CTX was given as a single dose on the 12th day of the experiment. Testis was scored according to spermatid density, giant cell formation, cells shed into tubules, maturation disorder, and atrophy. According to our biochemical findings, the high levels of total oxidant status (TOS), and the low levels of total antioxidant status (TAS) in the CTX group, which are oxidative stress markers, indicate the toxic effect of CTX, while the decrease in TOS levels and the increase in TAS levels in the kefir groups indicate the protective effect of kefir. In the CTX-administered group, tubules with impaired maturation and no spermatids were observed in the transverse section of the testicle, while in the kefir groups, the presence of near-normal tubule structures and tubule lumens despite CTX showed the protective effect of kefir. In our study, it was observed that kefir had a protective and curative effect on CTX-induced toxicity and oxidative stress and could be a strong protector., (© 2024. The Author(s).)

In this study, a biospectroscopic approach was reported for the detection of spectral changes and biomarkers for the diagnosis of colorectal cancer (CC) cases from different bodily fluids (blood plasma, blood serum, saliva and colonoscopy disinfection/wash fluids) by using attenuated total reflection-mid infrared (ATR-MIR) spectroscopy. To recognize the molecular level changes in the spectral characteristics of CC and their healthy/control (CH) groups, different multivariate data analyses (HCA, LDA, PCA and SIMCA) were successfully performed over the data of ATR-MIR spectroscopy. Two hundred specimens were characterized in detail over the data of spectral regions (4000-650 cm -1 and regions V-XXII). The findings revealed that significant changes were clearly observed in the concentrations of lipid, protein, nucleic acid and carbohydrate biomolecules for cancer cases based upon their necessity to overcome energy requirements. Supervised multivariate data methodology SIMCA, presented an excellent classification for the studied groups; similarly 100% of the specimens from different bodily fluids were correctly classified by supervised methodology LDA. As a result, the developed ATR-MIR methodology for the classification of CC and their healthy groups highlighted a rapid cancer diagnosis approach from different bodily fluids; therefore, it could be guide to make well decision before histopathological assessment and to screen CC populations existing in society., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

To investigate the effects of escin (ES) on acute damage induced by alkylating agent, experimental rats were injected with cyclophosphamide (CPM) to cause liver damage. The animals were divided into four groups: Control Group, CPM (200 mg/kg), ES (10 mg/kg), CPM, and ES Groups. Immunohistopathological, hepatic histopathological, and biochemical changes were analyzed. The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), malondyaldehyde (MDA), glutathion (GSH), total oxidant status (TOS) and total antioxidant status (TAS) in serum were all determined. Serum and immunohistopathology analysis revealed that MDA, ALT, AST, LDH, TOC and OSI, caspase-3 and Bax levels had increased while GSH, TAC, Bcl- 2 and OSI levels decreased in CPM Group when compared to Control Group. These findings appear to account for the severe damage detected. In the CPM + ES treated group, positive improvements were found in biochemical parameters as well as in cell-death and tissue-related damage parameters.The results show that ES considerably protects the rat liver against CPM-induced hepatotoxicity thanks to because of its anti-oxidant and anti-apoptotic properties.

This paper describes the feasibility of Attenuated total reflection-Fourier transform mid-infrared (ATR-MIR) spectroscopy method coupled with chemometrics for the rapid diagnostic approach and screening spectral changes for gastric and colon cancers from paraffin-embedded tissues. A total number of 82 tissue samples were analyzed by a simple ATR-MIR method combined with PCA, HCA, SIMCA and LDA methodologies. Spectral analyses showed significant differences for the molecular contents particularly about the lipid, nucleic acid, protein and other biomolecules in the samples of gastric cancer (GC) and colon cancer (CC) groups from their control/healthy groups. Significant changes in the characteristic of these molecules were only observed for cancer groups based upon the increment in their biosynthesis, and they could be utilized as diagnostic spectral biomarkers. Under the optimum conditions, SIMCA provided excellent classification for diseased and control groups, with 5% significance level. As well, 97.75% of the studied tissue samples were correctly discriminated on the basis of their origin by LDA. Consequently, the findings of this study highlighted the rapid diagnosis of gastric and colon cancer cases from paraffin-embedded tissues via ATR-MIR spectroscopy complemented with chemometrics., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Background/aim: Primary hyperparathyroidism (PHPT) is characterized by increased calcium (Ca) and parathyroid hormone (PTH) levels. Surgical removal of the culprit hyperfunctioning parathyroid gland is the preferred treatment. In this study, we aimed to determine whether PTH-washout or cytological examination of suspicious lesions was superior in MIBI-negative patients diagnosed with PHPT.Materials and methods: We retrospectively evaluated the medical records of 98 patients diagnosed with PHPT. Seventy-six patients who had positive parathyroid scintigraphy and who did not undergo parathyroidectomy in our center due to various reasons were excluded. We evaluated the remaining 22 patients with negative scintigraphy. Medical records including PTH levels in serum and washout fluid of fine-needle aspiration biopsy (FNAB), biochemical data, cytological results of FNAB, and histologic reports were reviewed.Results: The patients had a mean age of 50 +- 9 (31-72) years, serum Ca of 10.9 +- 0.5 (10.3-12.7) mg/dL, serum PTH of 285 +- 156 (107.2-679) pg/mL, and PTH of washout fluid of 19,523 +- 38,632 (1410-166,000) pg/mL. Cytological evaluation revealed insufficient material in 9 patients and cells of indeterminate origin in 4 patients.Conclusion: Our results showed that when evaluating ambiguous lesions on neck ultrasound, measuring the PTH level in washout fluid of FNAB is a reliable and effective method for diagnosis of parathyroid lesions and is superior to FNAB for localization.