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28 results on '"Peisker, Helga"'

4. Degradation of FATTY ACID EXPORT PROTEIN1 by RHOMBOID-LIKE PROTEASE11 contributes to cold tolerance in Arabidopsis

Author

John, Annalisa, primary, Krämer, Moritz, additional, Lehmann, Martin, additional, Kunz, Hans-Henning, additional, Aarabi, Fayezeh, additional, Alseekh, Saleh, additional, Fernie, Alisdair, additional, Sommer, Frederik, additional, Schroda, Michael, additional, Zimmer, David, additional, Mühlhaus, Timo, additional, Peisker, Helga, additional, Gutbrod, Katharina, additional, Dörmann, Peter, additional, Neunzig, Jens, additional, Philippar, Katrin, additional, and Neuhaus, H Ekkehard, additional

Published
2024
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7. Degradation of Fatty Acid Export Protein1 by Rhomboid-Like Protease11 Contributes to Cold Tolerance in Arabidopsis

Author

John, Annalisa, primary, Kraemer, Moritz, additional, Lehmann, Martin, additional, Kunz, Hans-Henning, additional, Aarabi, Fayezeh, additional, Alseekh, Saleh, additional, Fernie, Alisdair R, additional, Sommer, Frederik, additional, Schroda, Michael, additional, Zimmer, David, additional, Muehlhaus, Timo, additional, Peisker, Helga, additional, Gutbrod, Katharina, additional, Doermann, Peter, additional, Neunzig, Jens, additional, Philippar, Katrin, additional, and Neuhaus, H. Ekkehard, additional

Published
2023
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10. Epilepsy and nodding syndrome in association with an Onchocerca volvulus infection drive distinct immune profile patterns

Author

Arndts, Kathrin, primary, Kegele, Josua, additional, Massarani, Alain S., additional, Ritter, Manuel, additional, Wagner, Thomas, additional, Pfarr, Kenneth, additional, Lämmer, Christine, additional, Dörmann, Peter, additional, Peisker, Helga, additional, Menche, Dirk, additional, Al-Bahra, Mazen, additional, Prazeres da Costa, Clarissa, additional, Schmutzhard, Erich, additional, Matuja, William, additional, Hoerauf, Achim, additional, Layland-Heni, Laura E., additional, and Winkler, Andrea S., additional

Published
2023
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16. Additional file 3 of Urine metabolites for the identification of Onchocerca volvulus infections in patients from Cameroon

Author

Wewer, Vera, Peisker, Helga, Gutbrod, Katharina, Al-Bahra, Mazen, Menche, Dirk, Amambo, Ngongeh Glory, Fombad, Fanny F., Njouendou, Abdel Jelil, Pfarr, Kenneth, Wanji, Samuel, Hoerauf, Achim, and Dörmann, Peter

Abstract

Additional file 3: Figure S1. 1H-NMR and 13C-NMR spectra of N-D3-acetyltyramine-O,β-glucuronide (D3-NATOG) and intermediates of synthesis. a 1H-NMR spectrum of N-D3-acetyltyramine. b 13C-NMR spectrum of N-D3-acetyltyramine. c 1H-NMR spectrum of N-D3-acetyltyramine-O-(tri-O-acetyl-β-glucuronide methyl ester). d 13C-NMR spectrum of N-D3-acetyltyramine-O-(tri-O-acetyl-β-glucuronide methyl ester). e 1H-NMR spectrum of N-D3-acetyltyramine-O,β-glucuronide (D3-NATOG). f 13C-NMR spectrum of N-D3-acetyltyramine-O,β-glucuronide (D3-NATOG). g 1H-2D-NOESY spectrum of N-D3-acetyltyramine-O,β-glucuronide (D3-NATOG). This spectrum proves the exclusive presence of the β-anomer of the glycoside.

Published
2021
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19. 2‐Hydroxy‐phytanoyl‐CoA lyase (AtHPCL) is involved in phytol metabolism in Arabidopsis.

Author

Yang, Wentao, Gutbrod, Philipp, Gutbrod, Katharina, Peisker, Helga, Song, Xiaoning, Falz, Anna‐Lena, Meyer, Andreas J., and Dörmann, Peter

Subjects
ARABIDOPSIS, FATTY acid esters, DIOXYGENASES, METABOLISM, CHLOROPHYLL
Abstract

SUMMARY: During chlorophyll degradation, large amounts of the isoprenoid alcohol phytol are released. The pathway of phytol catabolism has been studied in humans, because chlorophyll is part of the human diet, but little is known for plants. In humans, phytanoyl‐CoA derived from phytol is degraded via α‐oxidation by phytanoyl‐CoA hydroxylase (PAHX) and 2‐hydroxy‐phytanoyl‐CoA lyase (HPCL). Arabidopsis contains two sequences homologous to the human proteins AtPAHX and AtHPCL. Insertional mutants of Arabidopsis (pahx, hpcl) were grown under N deprivation to stimulate chlorophyll breakdown or supplemented with phytol to increase the endogenous amount of phytol. During N deprivation, chlorophyll, phytol, phytenal, upstream metabolites of phytol breakdown, and tocopherol and fatty acid phytyl esters, alternative phytol‐derived lipids, accumulated in pahx and hpcl mutants, in line with the scenario that the mutations interfere with phytol degradation. AtHPCL was localized to the peroxisomes. Expression analysis of the AtHPCL sequence in the yeast Δpxp1 or Δmpo1 mutants followed by supplementation with 2‐hydroxy‐palmitic acid and enzyme assays of peroxisomal proteins from Col‐0 and hpcl plants with 2‐hydroxy‐stearoyl‐CoA revealed that AtHPCL harbors 2‐hydroxy‐acyl‐CoA lyase activity. The α‐dioxygenases αDOX1 and αDOX2 are involved in α‐oxidation of fatty acids and could be involved in an alternative pathway of phytol degradation. However, phytol‐related lipids in the αdox1, αdox2, or αdox1 αdox2 mutants were not altered compared with Col‐0, indicating that αDOX1 and αDOX2 are not involved in phytol degradation. These results demonstrate that phytol degradation in Arabidopsis involves α‐oxidation by AtPAHX and AtHPCL, but that it is independent of αDOX1/αDOX2. Significance Statement: The degradation and turnover of phytol, the hydrophobic anchor of chlorophyll, in plants is poorly understood. We show here that phytol degradation in Arabidopsis proceeds via α‐oxidation catalyzed by phytanoyl‐CoA hydroxylase (PAHX) and 2‐hydroxy‐phytanoyl‐CoA lyase (HPCL), but is independent of the α‐dioxygenases αDOX1 and αDOX2. Therefore, the pathway of phytol degradation is related to the respective human pathway. [ABSTRACT FROM AUTHOR]

Published
2022
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20. Surface wax esters contribute to drought tolerance in Arabidopsis

Author

Patwari, Payal, primary, Salewski, Veronika, additional, Gutbrod, Katharina, additional, Kreszies, Tino, additional, Dresen‐Scholz, Brigitte, additional, Peisker, Helga, additional, Steiner, Ulrike, additional, Meyer, Andreas J., additional, Schreiber, Lukas, additional, and Dörmann, Peter, additional

Published
2019
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27. Cyclic monoterpene mediated modulations of Arabidopsis thalianaphenotype

Author

Kriegs, Bettina, Jansen, Marcus, Hahn, Ka, Peisker, Helga, Šamajová, Olga, Beck, Martina, Braun, Silvia, Ulbrich, Andreas, Baluška, František, and Schulz, Margot

Abstract

Monoterpenes at high atmospheric concentrations are strong growth inhibitors in allelopathic interactions. Effects depend on dose, molecular structure of the monoterpene and on the species of the receiver plant. Stomata are among the first targets affected by camphor and menthol. Previously, it could be demonstrated that the compounds induce swelling of the protoplasts, prevent stomatal closure and enhance transpiration. In this study, we show that the block of stomatal closure is accompanied by changes to the cytoskeleton, which has a direct role in stomatal movements. Although MPK3 (MAP3 kinase) and ABF4 gene expressions are induced within six hours, stomatal closure is prevented. In contrast to ABF4, ABF2 (both transcription factors) is not induced. MPK3 and ABF4 both encode for proteins involved in the process of stomatal closure. The expression of PEPCase, an enzyme important for stomatal opening, is down regulated. The leaves develop stress symptoms, mirrored by transient changes in the expression profile of additional genes: lipoxygenase 2 (LOX2), CER5, CER6 (both important for wax production) and RD29B (an ABA inducible stress protein). Non-invasive methods showed a fast response of the plant to camphor fumigations both in a rapid decrease of the quantum yield and in the relative growth rate. Repeated exposures to the monoterpenes resulted finally in growth reduction and a stress related change in the phenotype. It is proposed that high concentrations or repeated exposure to monoterpenes led to irreversible damages, whereas low concentrations or short-term fumigations may have the potential to strengthen the plant fitness.

Published
2010
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28. Direct Infusion Mass Spectrometry for Complex Lipid Analysis.

Author

Gutbrod K, Peisker H, and Dörmann P

Subjects
Chromatography, Liquid methods, Glycerides chemistry, Lipidomics methods, Phospholipids analysis, Plant Leaves chemistry, Plants chemistry, Solid Phase Extraction methods, Tandem Mass Spectrometry methods, Triglycerides analysis, Lipids analysis, Lipids chemistry, Mass Spectrometry methods
Abstract

Direct infusion or "shotgun" mass spectrometry provides a fast strategy to measure different classes of lipids, combining rapid analysis and short idle time. In contrast to liquid chromatography-mass spectrometry (LC-MS), the lipids are infused into the mass spectrometer without prior separation by liquid chromatography. Ions are separated in the quadrupole of a tandem mass spectrometer, and after collision-induced dissociation fragments are quantified relative to internal standards in the third quadrupole or in the time-of-flight mass analyzer of a triple quadrupole or quadrupole time of flight (Q-TOF) mass spectrometer. Abundant lipids, that is, galactolipids and phospholipids in leaves, are measured in crude lipid extracts, while less abundant lipids can be measured after enrichment by solid-phase extraction. Here we describe protocols for the quantification of the major plant glycerolipids (galactolipids, phospholipids, diacylglycerol, and triacylglycerol) using nanospray direct infusion mass spectrometry. This provides a strategy for comprehensive, highly sensitive, high-throughput lipidomic analyses.

Published
2021
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