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15 results on '"Peiró-Mestres A"'

1. Diagnostic Performance of Six Rapid Antigen Tests for SARS-CoV-2

Author

Jessica Navero-Castillejos, Climent Casals-Pascual, Sofía Narváez, Genoveva Cuesta, Juan Carlos Hurtado, Mariana Fernandez, Mireia Navarro, Aida Peiró-Mestres, Ma Victoria Lasheras, Patricia Rodriguez, Andrea Pulgarín, Ma Ángeles Marcos, Jordi Vila, and Miguel Julián Martínez

Subjects
SARS-CoV-2, rapid antigen test, diagnosis, Microbiology, QR1-502
Abstract

ABSTRACT Microbiological diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a challenge. Although real-time reverse transcription PCR (RT-PCR) represents the gold standard method, strategies that allow rapid and simple diagnosis are necessary for the early identification of cases. In this study, we evaluated the diagnostic performance of six different commercial rapid antigen tests (Coronavirus antigen [Ag] rapid test cassette [Healgen Scientific, Houston, TX, USA], COVID-19 Ag FIA [Vircell, SD Biosensor Inc., Gyeonggi-do, Republic of Korea], Clinitest rapid COVID-19 antigen test [Siemens, Healthineers, Erlangen, Germany], SARS-CoV-2 rapid antigen test [SD Biosensor; Roche Diagnostics, Basel, Switzerland], Panbio COVID-19 Ag rapid test device [Abbott, Chicago, IL, USA], and SARS-CoV-2 test [MonLab, Barcelona, Spain]) in 130 nasopharyngeal swab samples tested previously by RT-PCR. The overall sensitivity of the rapid tests ranged from 65% to 79%, and the specificity was 100% for all of them. The sensitivity was higher for those samples with RT-PCR cycle threshold (CT) values below 25 and those from patients presenting within the first week of symptoms. The Siemens test showed the highest sensitivity for patients with high viral loads while the Vircell test performed better than the rest for CT values of ≥25. IMPORTANCE The rapid detection of people infected with SARS-CoV-2 is essential for a correct and effective control of the disease it causes. This process must be sensitive, fast, and simple, and it must be possible to carry out in any type of health center. Rapid antigen tests are the answer to this need. Knowing its ability to detect the virus in different stages of the disease is essential for a correct diagnosis, which is why this study has been carried out to evaluate the sensitivity and specificity of 6 different antigens tests in nasopharyngeal smear samples.

Published
2022
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2. Evaluation of a novel microfluidic immuno-magnetic agglutination assay method for detection of dengue virus NS1 antigen.

Author

Izaskun Alejo-Cancho, Jessica Navero-Castillejos, Aida Peiró-Mestres, Rosa Albarracín, Josep Barrachina, Alexander Navarro, Verónica Gonzalo, Víctor Pastor, José Muñoz, and Miguel J Martínez

Subjects
Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270
Abstract

BACKGROUND:Dengue virus (DENV) is the most important arbovirus worldwide, causing infections in endemic countries and returning travellers from these areas. Rapid diagnostic tests are needed to improve patient management and monitor local transmission. The detection of DENV non-structural protein 1 (NS1) is a useful tool for the diagnosis, but the currently available methods can be time consuming or lack sensitivity. The objective of our study was to evaluate a new rapid and semi-quantitative microfluidic DENV NS1 immuno-magnetic agglutination assay based on aggregation of magnetic nanoparticles detected by an electronic reader (Virotrack Dengue Acute and Blubox, Blusense diagnostics, Copenhagen, Denmark). METHODOLOGY/PRINCIPAL FINDINGS:A panel of 135 serum samples from travelers returning from dengue endemic countries was analyzed (74 DENV positive samples including the four DENV serotypes, 26 Zika virus positive samples, 25 chikungunya virus positive samples, 5 malaria positive samples and 5 negative samples). Samples were tested by three different antigen detection methods: SD Dengue NS1 Ag ELISA, SD BIOLINE Dengue Duo and ViroTrack Dengue Acute. The sensitivity observed for SD Dengue NS1 Ag ELISA, ViroTrack Dengue Acute and SD BIOLINE Dengue Duo was 97.2%, 91.1% and 68.1%, respectively. All methods showed high specificity (98.4% for ViroTrack Dengue Acute and 100% for both SD Dengue NS1 Ag ELISA and SD BIOLINE Dengue Duo). SD Dengue NS1 Ag ELISA and ViroTrack Dengue Acute only failed to detect samples positive for DENV-2. CONCLUSIONS/SIGNIFICANCE:ViroTrack Dengue Acute is a sensitive and specific assay for DENV NS1 detection. It provides faster results than the ELISA method and a better performance than the rapid immunochromatographic tests. ViroTrack Dengue Acute could represent a valuable tool for rapid diagnosis of DENV infections in returning travellers from endemic countries.

Published
2020
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3. Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain

Author

Jessica Navero-Castillejos, Rosa Benitez, Nuria Torner, José Muñoz, Daniel Camprubí-Ferrer, Aida Peiró-Mestres, Elena Sulleiro, Aroa Silgado, Verónica Gonzalo, Teresa Falgueras, Izaskun Alejo-Cancho, Montserrat Roldán, Virginia Plasencia, Rosa Albarracin, Josefa Perez, Alexander Navarro, Ana Calderón, Rosa Rubio, Mireia Navarro, Miguel Micó, Jaume Llaberia, María Navarro, Josep Barrachina, Anna Vilamala, Carmina Martí, María Ángeles Pulido, María Paz Sanchez-Seco, Ana Vazquez, Ana Martínez, Mireia Jané, and Miguel Julián Martínez

Subjects
dengue, molecular epidemiology, autochthonous transmission, surveillance, Microbiology, QR1-502
Abstract

Dengue is the most significant arbovirus worldwide and a public health threat to non-endemic areas in which Aedes vectors are present. Autochthonous dengue transmission has been reported in several European countries in the last decade. Infected travelers from endemic regions arriving to areas colonized by Aedes albopictus in Europe need to be monitored in surveillance and control programs. We aimed to perform molecular characterization of RT-PCR-positive dengue cases detected in Catalonia, northeastern Spain, from 2013 to 2018. The basic demographic information and the geographical regions of importation were also analyzed. One-hundred four dengue cases were studied (103 imported infections and the first autochthonous case in our region). The dengue virus strains detected were serotyped and genotyped using molecular methods, and phylogenetic analyses were conducted. All four dengue serotypes were detected in travelers, including up to 10 different genotypes, reflecting the global circulation of dengue in endemic areas. The primary travel-related case of the 2018 autochthonous transmission was not identified, but the molecular analysis revealed dengue serotype 1, genotype I of Asian origin. Our results highlight the diversity of imported dengue virus strains and the role of molecular epidemiology in supporting arbovirus surveillance programs.

Published
2021
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4. Frequent detection of monkeypox virus DNA in saliva, semen, and other clinical samples from 12 patients, Barcelona, Spain, May to June 2022

Author

Aida, Peiró-Mestres, Irene, Fuertes, Daniel, Camprubí-Ferrer, María Ángeles, Marcos, Anna, Vilella, Mireia, Navarro, Laura, Rodriguez-Elena, Josep, Riera, Alba, Català, Miguel J, Martínez, and Jose L, Blanco

Subjects
Epidemiology, Semen, Spain, Virology, DNA, Viral, Public Health, Environmental and Occupational Health, Humans, Monkeypox, Monkeypox virus, Saliva
Abstract

A monkeypox (MPX) outbreak has expanded worldwide since May 2022. We tested 147 clinical samples collected at different time points from 12 patients by real-time PCR. MPX DNA was detected in saliva from all cases, sometimes with high viral loads. Other samples were frequently positive: rectal swab (11/12 cases), nasopharyngeal swab (10/12 cases), semen (7/9 cases), urine (9/12 cases) and faeces (8/12 cases). These results improve knowledge on virus shedding and the possible role of bodily fluids in disease transmission.

Published
2022

5. Diagnostic Performance of Six Rapid Antigen Tests for SARS-CoV-2

Author

Navero-Castillejos, Jessica, primary, Casals-Pascual, Climent, additional, Narváez, Sofía, additional, Cuesta, Genoveva, additional, Hurtado, Juan Carlos, additional, Fernandez, Mariana, additional, Navarro, Mireia, additional, Peiró-Mestres, Aida, additional, Lasheras, Ma Victoria, additional, Rodriguez, Patricia, additional, Pulgarín, Andrea, additional, Marcos, Ma Ángeles, additional, Vila, Jordi, additional, and Martínez, Miguel Julián, additional

Published
2022
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6. Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain

Author

Navero-Castillejos, Jessica, primary, Benitez, Rosa, additional, Torner, Nuria, additional, Muñoz, José, additional, Camprubí-Ferrer, Daniel, additional, Peiró-Mestres, Aida, additional, Sulleiro, Elena, additional, Silgado, Aroa, additional, Gonzalo, Verónica, additional, Falgueras, Teresa, additional, Alejo-Cancho, Izaskun, additional, Roldán, Montserrat, additional, Plasencia, Virginia, additional, Albarracin, Rosa, additional, Perez, Josefa, additional, Navarro, Alexander, additional, Calderón, Ana, additional, Rubio, Rosa, additional, Navarro, Mireia, additional, Micó, Miguel, additional, Llaberia, Jaume, additional, Navarro, María, additional, Barrachina, Josep, additional, Vilamala, Anna, additional, Martí, Carmina, additional, Pulido, María Ángeles, additional, Sanchez-Seco, María Paz, additional, Vazquez, Ana, additional, Martínez, Ana, additional, Jané, Mireia, additional, and Martínez, Miguel Julián, additional

Published
2021
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7. Spread of a SARS-CoV-2 variant through Europe in the summer of 2020

Author

Hodcroft E.B., Zuber M., Nadeau S., Vaughan T.G., Crawford K.H.D., Althaus C.L., Reichmuth M.L., Bowen J.E., Walls A.C., Corti D., Bloom J.D., Veesler D., Mateo D., Hernando A., Comas I., González-Candelas F., Goig G.A., Chiner-Oms Á., Cancino-Muñoz I., López M.G., Torres-Puente M., Gomez-Navarro I., Jiménez-Serrano S., Ruiz-Roldán L., Bracho M.A., García-González N., Martínez-Priego L., Galán-Vendrell I., Ruiz-Hueso P., De Marco G., Ferrús M.L., Carbó-Ramírez S., D’Auria G., Coscollá M., Ruiz-Rodríguez P., Roig-Sena F.J., Sanmartín I., Garcia-Souto D., Pequeno-Valtierra A., Tubio J.M.C., Rodríguez-Castro J., Rabella N., Navarro F., Miró E., Rodríguez-Iglesias M., Galán-Sanchez F., Rodriguez-Pallares S., de Toro M., Escudero M.B., Azcona-Gutiérrez J.M., Alberdi M.B., Mayor A., García-Basteiro A.L., Moncunill G., Dobaño C., Cisteró P., García-de-Viedma D., Pérez-Lago L., Herranz M., Sicilia J., Catalán-Alonso P., Muñoz P., Muñoz-Cuevas C., Rodríguez-Rodríguez G., Alberola-Enguidanos J., Nogueira J.M., Camarena J.J., Rezusta A., Tristancho-Baró A., Milagro A., Martínez-Cameo N.F., Gracia-Grataloup Y., Martró E., Bordoy A.E., Not A., Antuori-Torres A., Benito R., Algarate S., Bueno J., del Pozo J.L., Boga J.A., Castelló-Abietar C., Rojo-Alba S., Alvarez-Argüelles M.E., Melon S., Aranzamendi-Zaldumbide M., Vergara-Gómez A., Fernández-Pinero J., Martínez M.J., Vila J., Rubio E., Peiró-Mestres A., Navero-Castillejos J., Posada D., Valverde D., Estévez-Gómez N., Fernandez-Silva I., de Chiara L., Gallego-García P., Varela N., Moreno R., Tirado M.D., Gomez-Pinedo U., Gozalo-Margüello M., Eliecer-Cano M., Méndez-Legaza J.M., Rodríguez-Lozano J., Siller M., Pablo-Marcos D., Oliver A., Reina J., López-Causapé C., Canut-Blasco A., Hernáez-Crespo S., Cordón M.L.A., Lecároz-Agara M.-C., Gómez-González C., Aguirre-Quiñonero A., López-Mirones J.I., Fernández-Torres M., Almela-Ferrer M.R., Gonzalo-Jiménez N., Ruiz-García M.M., Galiana A., Sanchez-Almendro J., Cilla G., Montes M., Piñeiro L., Sorarrain A., Marimón J.M., Gomez-Ruiz M.D., López-Hontangas J.L., González Barberá E.M., Navarro-Marí J.M., Pedrosa-Corral I., Sanbonmatsu-Gámez S., Pérez-González C., Chamizo-López F., Bordes-Benítez A., Navarro D., Albert E., Torres I., Gascón I., Torregrosa-Hetland C.J., Pastor-Boix E., Cascales-Ramos P., Fuster-Escrivá B., Gimeno-Cardona C., Ocete M.D., Medina-Gonzalez R., González-Cantó J., Martínez-Macias O., Palop-Borrás B., de Toro I., Mediavilla-Gradolph M.C., Pérez-Ruiz M., González-Recio Ó., Gutiérrez-Rivas M., Simarro-Córdoba E., Lozano-Serra J., Robles-Fonseca L., de Salazar A., Viñuela-González L., Chueca N., García F., Gómez-Camarasa C., Carvajal A., de la Puente R., Martín-Sánchez V., Fregeneda-Grandes J.-M., Molina A.J., Argüello H., Fernández-Villa T., Farga-Martí M.A., Domínguez-Márquez V., Costa-Alcalde J.J., Trastoy R., Barbeito-Castiñeiras G., Coira A., Pérez-del-Molino M.L., Aguilera A., Planas A.M., Soriano A., Fernandez-Cádenas I., Pérez-Tur J., Marcos M.Á., Moreno-Docón A., Viedma E., Mingorance J., Galán-Montemayor J.C., Parra-Grande M., Stadler T., Neher R.A., Swiss National Science Foundation, European Commission, University of Basel, ETH Zurich, National Institute of General Medical Sciences (US), National Institute of Allergy and Infectious Diseases (US), Burroughs Wellcome Fund, Instituto de Salud Carlos III, Consejo Superior de Investigaciones Científicas (España), European Research Council, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Howard Hughes Medical Institute, Hodcroft, Emma B. [0000-0002-0078-2212], Zuber, Moira [0000-0002-4275-8739], Nadeau, Sarah [0000-0003-1008-8918], Vaughan, Timothy G. [0000-0001-6220-2239], Crawford, Katharine H. D. [0000-0002-6223-4019], Althaus, Christian L. [0000-0002-5230-6760], Reichmuth, Martina L. [0000-0001-9345-851X], Bowen, John E. [0000-0003-3590-9727], Walls, Alexandra C. [0000-0002-9636-8330], Corti, Davide [0000-0002-5797-1364], Bloom, Jesse D. [0000-0003-1267-3408], Veesler, David [0000-0002-6019-8675], Mateo, David [0000-0002-1590-4163], Hernando de Castro, Alberto [0000-0003-1180-1068], Comas, Iñaki [0000-0001-5504-9408], González-Candelas, Fernando [0000-0002-0879-5798], Stadler, Tanja [0000-0001-6431-535X], Neher, Richard A. [0000-0003-2525-1407], Hodcroft, Emma B., Zuber, Moira, Nadeau, Sarah, Vaughan, Timothy G., Crawford, Katharine H. D., Althaus, Christian L., Reichmuth, Martina L., Bowen, John E., Walls, Alexandra C., Corti, Davide, Bloom, Jesse D., Veesler, David, Mateo, David, Hernando de Castro, Alberto, Comas, Iñaki, González-Candelas, Fernando, Stadler, Tanja, and Neher, Richard A.

Subjects
Phylogenetics, Sars-Cov-2, Viral infection, 0303 health sciences, 2019-20 coronavirus outbreak, Multidisciplinary, Coronavirus disease 2019 (COVID-19), SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 610 Medicine & health, 3. Good health, law.invention, 03 medical and health sciences, 0302 clinical medicine, Transmission (mechanics), Geography, 360 Social problems & social services, law, Development economics, 030217 neurology & neurosurgery, 030304 developmental biology
Abstract

Following its emergence in late 2019, the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)1,2 has been tracked via phylogenetic analysis of viral genome sequences in unprecedented detail3–5. While the virus spread globally in early 2020 before borders closed, intercontinental travel has since been greatly reduced. However, within Europe travel resumed in the summer of 2020. Here we report on a novel SARS-CoV-2 variant, 20E (EU1), that emerged in Spain in early summer, and subsequently spread across Europe. We find no evidence of increased transmissibility, but instead demonstrate how rising incidence in Spain, resumption of travel, and lack of effective screening and containment may explain the variant’s success. Despite travel restrictions, we estimate 20E (EU1) was introduced hundreds of times to European countries by summertime travelers, likely undermining local efforts to keep SARS-CoV-2 cases low. Our results demonstrate how a variant can rapidly become dominant even in absence of a substantial transmission advantage in favorable epidemiological settings. Genomic surveillance is critical to understanding how travel can impact SARS-CoV-2 transmission, and thus for informing future containment strategies as travel resumes., This work was supported by the Swiss National Science Foundation (SNSF) through grant numbers 31CA30 196046 (to RAN, EBH, CLA), 31CA30 196267 (to TS), European Union’s Horizon 2020 research and innovation programme - project EpiPose (No 101003688) (MLR, CLA), core funding by the University of Basel and ETH Zürich, the National Institute of General Medical Sciences (R01GM120553 to DV), the National Institute of Allergy and Infectious Diseases (DP1AI158186 and HHSN272201700059C to DV), a Pew Biomedical Scholars Award (DV), an Investigators in the Pathogenesis of Infectious Disease Awards from the Burroughs Wellcome Fund (DV and JDB), a Fast Grants (DV), and NIAID grants R01AI141707 (JDB) and F30AI149928 (KHDC). SeqCOVID-SPAIN is funded by the Instituto de Salud Carlos III project COV20/00140, Spanish National Research Council and ERC StG 638553 to IC and BFU2017-89594R from MICIN to FGC. JDB is an Investigator of the Howard Hughes Medical Institute.

Published
2021
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8. The first wave of the COVID-19 epidemic in Spain was associated with early introductions and fast spread of a dominating genetic variant

Author

López, Mariana G., Chiner-Oms, Álvaro, García de Viedma, Darío, Ruiz-Rodriguez, Paula, Bracho, Maria Alma, Cancino-Muñoz, Irving, D’Auria, Giuseppe, de Marco, Griselda, García-González, Neris, Goig, Galo Adrian, Gómez-Navarro, Inmaculada, Jiménez-Serrano, Santiago, Martinez-Priego, Llúcia, Ruiz-Hueso, Paula, Ruiz-Roldán, Lidia, Torres-Puente, Manuela, Alberola, Juan, Albert, Eliseo, Aranzamendi Zaldumbide, Maitane, Bea-Escudero, María Pilar, Boga, Jose Antonio, Bordoy, Antoni E., Canut-Blasco, Andrés, Carvajal, Ana, Cilla Eguiluz, Gustavo, Cordón Rodríguez, Maria Luz, Costa-Alcalde, José J., de Toro, María, de Toro Peinado, Inmaculada, del Pozo, Jose Luis, Duchêne, Sebastián, Fernández-Pinero, Jovita, Fuster Escrivá, Begoña, Gimeno Cardona, Concepción, González Galán, Verónica, Gonzalo Jiménez, Nieves, Hernáez Crespo, Silvia, Herranz, Marta, Lepe, José Antonio, López-Causapé, Carla, López-Hontangas, José Luis, Martín, Vicente, Martró, Elisa, Milagro Beamonte, Ana, Montes Ros, Milagrosa, Moreno-Muñoz, Rosario, Navarro, David, Navarro-Marí, José María, Not, Anna, Oliver, Antonio, Palop-Borrás, Begoña, Parra Grande, Mónica, Pedrosa-Corral, Irene, Pérez González, Maria Carmen, Pérez-Lago, Laura, Pérez-Ruiz, Mercedes, Piñeiro Vázquez, Luis, Rabella, Nuria, Rezusta, Antonio, Robles Fonseca, Lorena, Rodríguez-Villodres, Ángel, Sanbonmatsu-Gámez, Sara, Sicilia, Jon, Soriano, Alex, Tirado Balaguer, María Dolores, Torres, Ignacio, Tristancho, Alexander, Marimón, José María, Pérez-Tur, Jordi, Catalán-Alonso, Pilar, Suárez González, Julia, Muñoz, Patricia, Ruiz-Rodríguez, Paula, Bracho, María Alma, Martínez Priego, Llúcia, Galán-Vendrell, Inmaculada, De Marco, Griselda, Ferrús-Abad, María Loreto, Carbó-Ramírez, Sandra, Nogueira, Jose Miguel, Camarena, Juan José, Martínez Expósito, Óscar, Antona Urieta, Nerea, Castelló-Abietar, Cristian, Rojo-Alba, Susana, Álvarez-Argüelles, Marta Elena, Melón, Santiago, Antuori, Adrián, Fernández-Navarro, Anabel, Lecaroz Agara, Maria Concepción, Gómez-González, Carmen, Aguirre-Quiñonero, Amaia, López-Mirones, José Israel, Fernández-Torres, Marina, Almela-Ferrer, Maria Rosario, Fregeneda-Grandes, Juan Miguel, Argüello, Héctor, Sorarrain, Ane, Trastoy, Rocío, Barbeito Castiñeiras, Gema, Coira, Amparo, Pérez del Molino, María Luisa, Aguilera, Antonio, Mediavilla Gradolph, Maria Concepción, Fernández-Alonso, Mirian, González-Recio, Oscar, Gutiérrez-Rivas, Mónica, Jiménez Clavero, Miguel Ángel, Ocete Mochón, María Dolores, Medina-Gonzalez, Rafael, Reina, Jordi, Gómez-Ruiz, Maria Dolores, Gonzalez-Barbera, Eva M., Molina, Antonio J., Fernandez-Villa, Tania, Martínez-Cameo, Nieves Felisa, Gracia-Grataloup, Yolanda, Tirado Balaguer, Maria Dolores, Gómez Alonso, Bárbara, Arjona Zaragozí, Francisco José, Chamizo López, Francisco Javier, Bordes-Benítez, Ana, Rabella, Núria, Navarro, Ferran, Miró, Elisenda, Simarro Córdoba, Encarnación, Lozano-Serra, Julia, Soriano, Álex, Roig Sena, Francisco Javier, Vanaclocha Luna, Hermelinda, Sanmartín, Isabel, García-Souto, Daniel, Pequeño-Valtierra, Ana, Tubio, Jose M. C., Temes, Javier, Rodríguez-Castro, Jorge, Santamarina García, Martín, Rodríguez-Iglesias, Manuel, Galán-Sanchez, Fátima, Rodríguez-Pallares, Salud, Azcona-Gutiérrez, José Manuel, Blasco-Alberdi, Miriam, Mayor, Alfredo, García-Basteiro, Alberto L., Moncunill, Gemma, Dobaño, Carlota, Cisteró, Pau, Mitjà, Oriol, González-Beiras, Camila, Vall-Mayans, Martí, Corbacho-Monné, Marc, Alemany, Andrea, Muñoz-Cuevas, Cristina, Rodríguez-Rodríguez, Guadalupe, Benito, Rafael, Algarate, Sonia, Bueno, Jessica, Vergara-Gómez, Andrea, Martínez, Miguel J., Vila, Jordi, Rubio, Elisa, Peiró-Mestres, Aida, Navero-Castillejos, Jessica, Posada, David, Valverde, Diana, Estévez, Nuria, Fernández-Silva, Iria, de Chiara, Loretta, Gallego-García, Pilar, Varela, Nair, Gómez-Pinedo, Ulises, Gozalo-Margüello, Mónica, Cano García, Maria Eliecer, Méndez-Legaza, José Manuel, Rodríguez-Lozano, Jesus, Siller, María, Pablo-Marcos, Daniel, Ruiz-García, Maria Montserrat, Galiana, Antonio, Sánchez-Almendro, Judith, Gascón Ros, Maria Isabel, Torregrosa-Hetland, Cristina Juana, Pastor Boix, Eva María, Cascales Ramos, Paloma, Garcinuño Enríquez, Pedro Luis, Raga Borja, Salvador, González Cantó, Julia, Martínez Macias, Olalla, de Salazar, Adolfo, Viñuela González, Laura, Chueca, Natalia, García, Federico, Gómez-Camarasa, Cristina, Farga Martí, Amparo, Falcón, Rocío, Domínguez-Márquez, Victoria, Planas, Anna M., Fernández-Cádenas, Israel, Marcos, Maria Ángeles, Ezpeleta, Carmen, Navascués, Ana, Miqueleiz Zapatero, Ana, Segovia, Manuel, Moreno-Docón, Antonio, Viedma, Esther, Recio Martínez, Raúl, Muñoz-Gallego, Irene, Gonzalez-Bodi, Sara, Folgueira, Maria Dolores, Mingorance, Jesús, Dahdouh, Elias, Lázaro-Perona, Fernando, Rodríguez-Tejedor, María, Romero-Gómez, María Pilar, García-Rodríguez, Julio, Galán, Juan Carlos, Rodríguez-Dominguez, Mario, Martínez-García, Laura, Abreu Di Berardino, Melanie, Ponce-Alonso, Manuel, González-Alba, Jose Maria, Sanz-Muñoz, Ivan, Pérez San José, Diana, Gil Fortuño, Maria, Bellido-Blasco, Juan B., Yagüe Muñoz, Alberto, Hernández Pérez, Noelia, Buj Jordá, Helena, Pérez Olaso, Óscar, González Praetorius, Alejandro, Martínez Ramírez, Nora Mariela, Ramírez Marinero, Aida, Padilla León, Eduardo, Vilas Basil, Alba, Canal Aranda, Mireia, Bernet Sánchez, Albert, Bellés Bellés, Alba, López González, Eric, Prats Sánchez, Iván, García-González, Mercè, Martínez-Lirola, Miguel José, Rodríguez Maresca, Manuel Ángel, Cabezas Fernández, Maria Teresa, Carrillo Gil, María Eugenia, Ventero Martín, Maria Paz, Molina Pardines, Carmen, Orta Mira, Nieves, Navarro Cots, María, Vidal Catalá, Inmaculada, García Nava, Isabel, Illescas Fernández-Bermejo, Soledad, Martínez-Alarcón, José, Torres-Narbona, Marta, Colmenarejo, Cristina, García-Agudo, Lidia, Pérez García, Jorge A., Yago López, Martín, Goberna Bravo, María Ángeles, Simón García, Victoria, Llop Furquet, Gonzalo, Iranzo Tatay, Agustín, Moreno-Marro, Sandra, Lozano Rodríguez, Noelia, Broseta Tamarit, Amparo, Badiola Díez, Juan José, Martínez-Ramírez, Amparo, Dopazo, Ana, Callejas, Sergio, Benguría, Alberto, Aguado, Begoña, Alcamí, Antonio, Bermejo Bermejo, Marta, Ramos-Ruíz, Ricardo, Fernández Soria, Víctor Manuel, Simón Soria, Fernando, Roig Cardells, Mercedes, Coscolla, Mireia, González-Candelas, Fernando, Comas, Iñaki, Instituto de Salud Carlos III, Consejo Superior de Investigaciones Científicas (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Research Council, European Commission, Generalitat Valenciana, Chiner-Oms, Álvaro [0000-0002-0463-0101], Ruiz-Rodríguez, Paula [0000-0003-0727-5974], D'Auria, Giuseppe [0000-0003-0672-2541], Gómez-Navarro, Inmaculada [0000-0002-5235-7365], Jiménez Serrano, Santiago [0000-0003-2917-6053], Aranzamendi Zaldumbide, Maitane [0000-0003-2432-7078], Boga, José Antonio [0000-0002-5500-9972], Bordoy, Antoni E. [0000-0002-1165-542X], Costa-Alcalde, José J. [0000-0002-1916-2216], Toro Peinado, Inmaculada de [0000-0002-8151-3275], López-Causapé, Carla [0000-0003-2228-2005], Martín, Vicente [0000-0003-0552-2804], Moreno-Muñoz, Rosario [0000-0002-0185-5612], Parra Grande, Mónica [0000-0002-8330-7467], Balaguer, María Dolores [0000-0003-3808-7300], Coscolla, Mireia [0000-0003-0752-0538], González-Candelas, Fernando [0000-0002-0879-5798], Comas, Iñaki [0000-0001-5504-9408], Ministerio de Ciencia (España), Generalitat Valenciana (España), Chiner-Oms, Álvaro, Ruiz-Rodríguez, Paula, D'Auria, Giuseppe, Gómez-Navarro, Inmaculada, Jiménez Serrano, Santiago, Aranzamendi Zaldumbide, Maitane, Boga, José Antonio, Bordoy, Antoni E., Costa-Alcalde, José J., Toro Peinado, Inmaculada de, López-Causapé, Carla, Martín, Vicente, Moreno-Muñoz, Rosario, Parra Grande, Mónica, Balaguer, María Dolores, Coscolla, Mireia, González-Candelas, Fernando, Comas, Iñaki, and Marimon, Jose Maria

Subjects
DYNAMICS, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Physical Distancing, Biology, Severity of Illness Index, Article, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Pandemic, Quarantine, Genetics, Humans, Clade, Phylogeny, 030304 developmental biology, 0303 health sciences, Models, Statistical, SARS-CoV-2, Incidence (epidemiology), Incidence, Genetic variants, COVID-19, Genomics, New variant, Dynamics, Virus, 3. Good health, Spain, Communicable Disease Control, VIRUS, 030217 neurology & neurosurgery, Demography
Abstract

SeqCOVID-Spain consortium: Álvaro Chiner-Oms, Irving Cancino-Muñoz, Mariana G. López, Manuela Torres-Puente, Inmaculada Gómez-Navarro, Santiago Jiménez-Serrano, Jordi Pérez-Tur, Darío García de Viedma, Laura Pérez-Lago, Marta Herranz, Jon Sicilia, Pilar Catalán-Alonso, Julia Suárez González, Patricia Muñoz, Mireia Coscolla, Paula Ruiz-Rodríguez, Fernando González-Candelas, Iñaki Comas, Lidia Ruiz-Roldán, María Alma Bracho, Neris García-González, Llúcia Martínez Priego, Inmaculada Galán-Vendrell, Paula Ruiz-Hueso, Griselda De Marco, María Loreto Ferrús-Abad, Sandra Carbó-Ramírez, Giuseppe D’Auria, Galo Adrian Goig, Juan Alberola, Jose Miguel Nogueira, Juan José Camarena, David Navarro, Eliseo Albert, Ignacio Torres, Maitane Aranzamendi Zaldumbide, Óscar Martínez Expósito, Nerea Antona Urieta, María de Toro, María Pilar Bea-Escudero, Jose Antonio Boga, Cristian Castelló-Abietar, Susana Rojo-Alba, Marta Elena Álvarez-Argüelles, Santiago Melón, Elisa Martró, Antoni E. Bordoy, Anna Not, Adrián Antuori, Anabel Fernández-Navarro, Andrés Canut-Blasco, Silvia Hernáez Crespo, Maria Luz Cordón Rodríguez, Maria Concepción Lecaroz Agara, Carmen Gómez-González, Amaia Aguirre-Quiñonero, José Israel López-Mirones, Marina Fernández-Torres, Maria Rosario Almela-Ferrer, Ana Carvajal, Juan Miguel Fregeneda-Grandes, Héctor Argüello, Gustavo Cilla Eguiluz, Milagrosa Montes Ros, Luis Piñeiro Vázquez, Ane Sorarrain, José María Marimón, José J. Costa-Alcalde, Rocío Trastoy, Gema Barbeito Castiñeiras, Amparo Coira, María Luisa Pérez del Molino, Antonio Aguilera, Begoña Palop-Borrás, Inmaculada de Toro Peinado, Maria Concepción Mediavilla Gradolph, Mercedes Pérez-Ruiz, Mirian Fernández-Alonso, Jose Luis del Pozo, Oscar González-Recio, Mónica Gutiérrez-Rivas, Jovita Fernández-Pinero, Miguel Ángel Jiménez Clavero, Begoña Fuster Escrivá, Concepción Gimeno Cardona, María Dolores Ocete Mochón, Rafael Medina-Gonzalez, José Antonio Lepe, Verónica González Galán, Ángel Rodríguez-Villodres, Nieves Gonzalo Jiménez, Jordi Reina, Carla López-Causapé, Maria Dolores Gómez-Ruiz, Eva M. Gonzalez-Barbera, José Luis López-Hontangas, Vicente Martín, Antonio J. Molina, Tania Fernandez-Villa, Ana Milagro Beamonte, Nieves Felisa Martínez-Cameo, Yolanda Gracia-Grataloup, Rosario Moreno-Muñoz, Maria Dolores Tirado Balaguer, José María Navarro-Marí, Irene Pedrosa-Corral, Sara Sanbonmatsu-Gámez, Antonio Oliver, Mónica Parra Grande, Bárbara Gómez Alonso, Francisco José Arjona Zaragozí, Maria Carmen Pérez González, Francisco Javier Chamizo López, Ana Bordes-Benítez, Núria Rabella, Ferran Navarro, Elisenda Miró, Antonio Rezusta, Alexander Tristancho, Encarnación Simarro Córdoba, Julia Lozano-Serra, Lorena Robles Fonseca, Álex Soriano, Francisco Javier Roig Sena, Hermelinda Vanaclocha Luna, Isabel Sanmartín, Daniel García-Souto, Ana Pequeño-Valtierra, Jose M. C. Tubio, Javier Temes, Jorge Rodríguez-Castro, Martín Santamarina García, Manuel Rodríguez-Iglesias, Fátima Galán-Sanchez, Salud Rodríguez-Pallares, José Manuel Azcona-Gutiérrez, Miriam Blasco-Alberdi, Alfredo Mayor, Alberto L. García-Basteiro, Gemma Moncunill, Carlota Dobaño, Pau Cisteró, Oriol Mitjà, Camila González-Beiras, Martí Vall-Mayans, Marc Corbacho-Monné, Andrea Alemany, Cristina Muñoz-Cuevas, Guadalupe Rodríguez-Rodríguez, Rafael Benito, Sonia Algarate, Jessica Bueno, Andrea Vergara-Gómez, Miguel J. Martínez, Jordi Vila, Elisa Rubio, Aida Peiró-Mestres, Jessica Navero-Castillejos, David Posada, Diana Valverde, Nuria Estévez, Iria Fernández-Silva, Loretta de Chiara, Pilar Gallego-García, Nair Varela, Ulises Gómez-Pinedo, Mónica Gozalo-Margüello, Maria Eliecer Cano García, José Manuel Méndez-Legaza, Jesus Rodríguez-Lozano, María Siller, Daniel Pablo-Marcos, Maria Montserrat Ruiz-García, Antonio Galiana, Judith Sánchez-Almendro, Maria Isabel Gascón Ros, Cristina Juana Torregrosa-Hetland, Eva María Pastor Boix, Paloma Cascales Ramos, Pedro Luis Garcinuño Enríquez, Salvador Raga Borja, Julia González Cantó, Olalla Martínez Macias, Adolfo de Salazar, Laura Viñuela González, Natalia Chueca, Federico García, Cristina Gómez-Camarasa, Amparo Farga Martí, Rocío Falcón, Victoria Domínguez-Márquez, Anna M. Planas, Israel Fernández-Cádenas, Maria Ángeles Marcos, Carmen Ezpeleta, Ana Navascués, Ana Miqueleiz Zapatero, Manuel Segovia, Antonio Moreno-Docón, Esther Viedma, Raúl Recio Martínez, Irene Muñoz-Gallego, Sara Gonzalez-Bodi, Maria Dolores Folgueira, Jesús Mingorance, Elias Dahdouh, Fernando Lázaro-Perona, María Rodríguez-Tejedor, María Pilar Romero-Gómez, Julio García-Rodríguez, Juan Carlos Galán, Mario Rodríguez-Dominguez, Laura Martínez-García, Melanie Abreu Di Berardino, Manuel Ponce-Alonso, Jose Maria González-Alba, Ivan Sanz-Muñoz, Diana Pérez San José, Maria Gil Fortuño, Juan B. Bellido-Blasco, Alberto Yagüe Muñoz, Noelia Hernández Pérez, Helena Buj Jordá, Óscar Pérez Olaso, Alejandro González Praetorius, Nora Mariela Martínez Ramírez, Aida Ramírez Marinero, Eduardo Padilla León, Alba Vilas Basil, Mireia Canal Aranda, Albert Bernet Sánchez, Alba Bellés Bellés, Eric López González, Iván Prats Sánchez, Mercè García-González, Miguel José Martínez-Lirola, Manuel Ángel Rodríguez Maresca, Maria Teresa Cabezas Fernández, María Eugenia Carrillo Gil, Maria Paz Ventero Martín, Carmen Molina Pardines, Nieves Orta Mira, María Navarro Cots, Inmaculada Vidal Catalá, Isabel García Nava, Soledad Illescas Fernández-Bermejo, José Martínez-Alarcón, Marta Torres-Narbona, Cristina Colmenarejo, Lidia García-Agudo, Jorge A. Pérez García, Martín Yago López, María Ángeles Goberna Bravo, Victoria Simón García, Gonzalo Llop Furquet, Agustín Iranzo Tatay, Sandra Moreno-Marro, Noelia Lozano Rodríguez, Amparo Broseta Tamarit, Juan José Badiola Díez, Amparo Martínez-Ramírez, Ana Dopazo, Sergio Callejas, Alberto Benguría, Begoña Aguado, Antonio Alcamí, Marta Bermejo Bermejo, Ricardo Ramos-Ruíz, Víctor Manuel Fernández Soria, Fernando Simón Soria & Mercedes Roig Cardells, The coronavirus disease 2019 (COVID-19) pandemic has affected the world radically since 2020. Spain was one of the European countries with the highest incidence during the first wave. As a part of a consortium to monitor and study the evolution of the epidemic, we sequenced 2,170 samples, diagnosed mostly before lockdown measures. Here, we identified at least 500 introductions from multiple international sources and documented the early rise of two dominant Spanish epidemic clades (SECs), probably amplified by superspreading events. Both SECs were related closely to the initial Asian variants of SARS-CoV-2 and spread widely across Spain. We inferred a substantial reduction in the effective reproductive number of both SECs due to public-health interventions (Re, This work was mainly funded by the Instituto de Salud Carlos III project COV20/00140, with additional funding by Spanish National Research Council project CSIC-COV19-021, Ministerio de Ciencia project PID2019-104477RB-100, ERC StG 638553 and ERC CoG 101001038 to I.C., and BFU2017-89594R to F.G.C. M.C. is supported by Ramón y Cajal program from Ministerio de Ciencia and grants RTI2018-094399-A-I00 and Generalitat Valenciana (Regional Government) project SEJI/2019/011. We gratefully acknowledge Hospital Universitari Vall d’Hebron, Instituto de Salud Carlos III, IrsiCaixa AIDS Research Lab and all the international researchers and institutions that submitted sequenced SARS-CoV-2 genomes to the GISAID’s EpiCov Database (Supplementary Table 1), as an important part of our analyses has been made possible by the sharing of their work. We also thank Unidad de Bioinformática y Estadística, Centro de Investigación Príncipe Felipe, for allowing us to use the Computer Cluster to perform some of the bioinformatic analysis.

Published
2021

9. Evaluation of a novel microfluidic immuno-magnetic agglutination assay method for detection of dengue virus NS1 antigen

Author

Victor Pastor, Verónica Gonzalo, Jessica Navero-Castillejos, Miguel J. Martínez, Rosa Albarracin, Josep Barrachina, Izaskun Alejo-Cancho, Aida Peiró-Mestres, Jose Muñoz, and Alexander Navarro

Subjects
RNA viruses, 0301 basic medicine, Viral Diseases, viruses, RC955-962, Artificial Gene Amplification and Extension, Viral Nonstructural Proteins, Dengue virus, Pathology and Laboratory Medicine, medicine.disease_cause, Polymerase Chain Reaction, Dengue fever, Zika virus, Dengue, 0302 clinical medicine, Arctic medicine. Tropical medicine, Medicine and Health Sciences, Nanotechnology, Chikungunya, Enzyme-Linked Immunoassays, Antigens, Viral, Chikungunya Virus, Nanopartícules, biology, Transmission (medicine), virus diseases, Microfluidic Analytical Techniques, Infeccions, Infectious Diseases, Medical Microbiology, Arboviral Infections, Viral Pathogens, Viruses, Engineering and Technology, Pathogens, Public aspects of medicine, RA1-1270, Research Article, Alphaviruses, 030231 tropical medicine, Research and Analysis Methods, Infections, Microbiology, Arbovirus, Virus, Togaviruses, 03 medical and health sciences, Diagnostic Medicine, medicine, Immunoassays, Molecular Biology Techniques, Microbial Pathogens, Molecular Biology, Biology and life sciences, Flaviviruses, Immunomagnetic Separation, business.industry, Organisms, Public Health, Environmental and Occupational Health, Reverse Transcriptase-Polymerase Chain Reaction, Dengue Virus, biochemical phenomena, metabolism, and nutrition, medicine.disease, biology.organism_classification, Virology, Vector-Borne Diseases, 030104 developmental biology, Immunologic Techniques, Nanoparticles, business, Malaria
Abstract

Background Dengue virus (DENV) is the most important arbovirus worldwide, causing infections in endemic countries and returning travellers from these areas. Rapid diagnostic tests are needed to improve patient management and monitor local transmission. The detection of DENV non-structural protein 1 (NS1) is a useful tool for the diagnosis, but the currently available methods can be time consuming or lack sensitivity. The objective of our study was to evaluate a new rapid and semi-quantitative microfluidic DENV NS1 immuno-magnetic agglutination assay based on aggregation of magnetic nanoparticles detected by an electronic reader (Virotrack Dengue Acute and Blubox, Blusense diagnostics, Copenhagen, Denmark). Methodology/Principal findings A panel of 135 serum samples from travelers returning from dengue endemic countries was analyzed (74 DENV positive samples including the four DENV serotypes, 26 Zika virus positive samples, 25 chikungunya virus positive samples, 5 malaria positive samples and 5 negative samples). Samples were tested by three different antigen detection methods: SD Dengue NS1 Ag ELISA, SD BIOLINE Dengue Duo and ViroTrack Dengue Acute. The sensitivity observed for SD Dengue NS1 Ag ELISA, ViroTrack Dengue Acute and SD BIOLINE Dengue Duo was 97.2%, 91.1% and 68.1%, respectively. All methods showed high specificity (98.4% for ViroTrack Dengue Acute and 100% for both SD Dengue NS1 Ag ELISA and SD BIOLINE Dengue Duo). SD Dengue NS1 Ag ELISA and ViroTrack Dengue Acute only failed to detect samples positive for DENV-2. Conclusions/Significance ViroTrack Dengue Acute is a sensitive and specific assay for DENV NS1 detection. It provides faster results than the ELISA method and a better performance than the rapid immunochromatographic tests. ViroTrack Dengue Acute could represent a valuable tool for rapid diagnosis of DENV infections in returning travellers from endemic countries., Author summary Dengue virus is a human pathogen that causes millions of infections each year. It can be found in nearly all tropical areas, and people living in those regions and travelers who visit them are at risk of disease. The symptoms may vary from a mild flu-like disease to severe hemorrhagic symptoms. Accurate diagnosis of dengue is important for a better management of the disease and for epidemiological surveillance. Detection of DENV antigen is a useful tool to diagnose DENV infections. However, currently available techniques are not rapid (ELISA assays) or not accurate enough (immunochromatographic tests). Here, we evaluate a new antigen detection technique based on aggregation of magnetic nanoparticles. The method combines a short time to results (12 minutes) with a high diagnostic performance. We therefore conclude that this new technique could be a useful tool for the diagnosis of dengue.

Published
2020

11. Evaluation of a novel microfluidic immuno-magnetic agglutination assay method for detection of dengue virus NS1 antigen

Author

Alejo-Cancho, Izaskun, primary, Navero-Castillejos, Jessica, additional, Peiró-Mestres, Aida, additional, Albarracín, Rosa, additional, Barrachina, Josep, additional, Navarro, Alexander, additional, Gonzalo, Verónica, additional, Pastor, Víctor, additional, Muñoz, José, additional, and Martínez, Miguel J., additional

Published
2020
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12. Persistence of Zika virus in semen 93 days after the onset of symptoms

Author

Aida Peiró Mestres, Inés Oliveira Souto, Jose Muñoz Gutiérrez, Izaskun Alejo-Cancho, Joaquim Gascón Brustenga, and Miguel J. Martinez Yoldi

Subjects
Adult, Male, 0301 basic medicine, Microbiology (medical), Time Factors, Sexual transmission, viruses, Semen, Malalties víriques, Persistence (computer science), Zika virus, Serology, 03 medical and health sciences, 0302 clinical medicine, Humans, 030212 general & internal medicine, Aedes, biology, Zika Virus Infection, Zika Virus, Venezuela, biology.organism_classification, Virology, 030104 developmental biology, Viral persistence, Virus diseases
Abstract

INTRODUCTION: Zika virus is mainly transmitted through the bites of infected Aedes mosquitoes, although mother-to-child and sexual transmission have also been described. The presence of Zika virus in semen after infection seems to be not uncommon, but the duration of viral persistence has not been well-determined. METHODS: Molecular, serological and cell culture methods were used for the diagnosis and follow up of a case of Zika virus infection imported from Venezuela. Serial samples of serum, urine and semen were analyzed to investigate the persistence of the Zika virus. RESULTS: Zika virus was detected in semen samples up to 93 days after the onset of symptoms. CONCLUSIONS: Our results confirm the persistence of Zika virus in semen samples for long periods after infection.

Published
2018
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14. Spread of a SARS-CoV-2 variant through Europe in the summer of 2020

Author

Medicina Preventiva, Hodcroft, Emma B., Zuber, Moira, Nadeau, Sarah, Vaughan, Timothy G., Crawford, Katharine H. D., Althaus, Christian L., Reichmuth, Martina L., Bowen, John E., Walls, Alexandra C., Corti, Davide, Bloom, Jesse D., Veesler, David, Mateo, David, Hernando, Alberto, Comas, Iñaki, González-Candelas, Fernando, Goig, Galo Adrian, Chiner-Oms, Álvaro, Cancino-Muñoz, Irving, López, Mariana Gabriela, Torres-Puente, Manuela, Gomez-Navarro, Inmaculada, Jiménez-Serrano, Santiago, Ruiz-Roldán, Lidia, Bracho, María Alma, García-González, Neris, Martínez-Priego, Llúcia, Galán-Vendrell, Inmaculada, Ruiz-Hueso, Paula, De Marco, Griselda, Ferrús, Maria Loreto, Carbó-Ramírez, Sandra, D’Auria, Giuseppe, Coscollá, Mireia, Ruiz-Rodríguez, Paula, Roig-Sena, Francisco Javier, Sanmartín, Isabel, Garcia-Souto, Daniel, Pequeno-Valtierra, Ana, Tubio, Jose M. C., Rodríguez-Castro, Jorge, Rabella, Nuria, Navarro, Ferrán, Miró, Elisenda, Rodríguez-Iglesias, Manuel, Galán-Sanchez, Fátima, Rodriguez-Pallares, Salud, de Toro, María, Escudero, María Bea, Azcona-Gutiérrez, José Manuel, Alberdi, Miriam Blasco, Mayor, Alfredo, García-Basteiro, Alberto L., Moncunill, Gemma, Dobaño, Carlota, Cisteró, Pau, García-de-Viedma, Darío, Pérez-Lago, Laura, Herranz, Marta, Sicilia, Jon, Catalán-Alonso, Pilar, Muñoz, Patricia, Muñoz-Cuevas, Cristina, Rodríguez-Rodríguez, Guadalupe, Alberola-Enguidanos, Juan, Nogueira, Jose Miguel, Camarena, Juan José, Rezusta, Antonio, Tristancho-Baró, Alexander, Milagro, Ana, Martínez-Cameo, Nieves Felisa, Gracia-Grataloup, Yolanda, Martró, Elisa, Bordoy, Antoni E., Not, Anna, Antuori-Torres, Adrián, Benito, Rafael, Algarate, Sonia, Bueno, Jessica, del Pozo, Jose Luis, Boga, Jose Antonio, Castelló-Abietar, Cristián, Rojo-Alba, Susana, Alvarez-Argüelles, Marta Elena, Melon, Santiago, Aranzamendi-Zaldumbide, Maitane, Vergara-Gómez, Andrea, Fernández-Pinero, Jovita, Martínez, Miguel J., Vila, Jordi, Rubio, Elisa, Peiró-Mestres, Aida, Navero-Castillejos, Jessica, Posada, David, Valverde, Diana, Estévez-Gómez, Nuria, Fernandez-Silva, Iria, de Chiara, Loretta, Gallego-García, Pilar, Varela, Nair, Moreno, Rosario, Tirado, Maria Dolores, Gomez-Pinedo, Ulises, Gozalo-Margüello, Mónica, Eliecer-Cano, Maria, Méndez-Legaza, José Manuel, Rodríguez-Lozano, Jesus, Siller, María, Pablo-Marcos, Daniel, Oliver, Antonio, Reina, Jordi, López-Causapé, Carla, Canut-Blasco, Andrés, Hernáez-Crespo, Silvia, Cordón, Maria Luz A., Lecároz-Agara, María-Concepción, Gómez-González, Carmen, Aguirre-Quiñonero, Amaia, López-Mirones, José Israel, Fernández-Torres, Marina, Almela-Ferrer, Maria Rosario, Gonzalo-Jiménez, Nieves, Ruiz-García, Maria Montserrat, Galiana, Antonio, Sanchez-Almendro, Judith, Cilla, Gustavo, Montes, Milagrosa, Piñeiro, Luis, Sorarrain, Ane, Marimón, José María, Gomez-Ruiz, Maria Dolores, López-Hontangas, José Luis, González Barberá, Eva M., Navarro-Marí, José María, Pedrosa-Corral, Irene, Sanbonmatsu-Gámez, Sara, Pérez-González, Carmen, Chamizo-López, Francisco, Bordes-Benítez, Ana, Navarro, David, Albert, Eliseo, Torres, Ignacio, Gascón, Isabel, Torregrosa-Hetland, Cristina Juana, Pastor-Boix, Eva, Cascales-Ramos, Paloma, Fuster-Escrivá, Begoña, Gimeno-Cardona, Concepción, Ocete, María Dolores, Medina-Gonzalez, Rafael, González-Cantó, Julia, Martínez-Macias, Olalla, Palop-Borrás, Begoña, de Toro, Inmaculada, Mediavilla-Gradolph, Maria Concepción, Pérez-Ruiz, Mercedes, González-Recio, Óscar, Gutiérrez-Rivas, Mónica, Simarro-Córdoba, Encarnación, Lozano-Serra, Julia, Robles-Fonseca, Lorena, de Salazar, Adolfo, Viñuela-González, Laura, Chueca, Natalia, García, Federico, Gómez-Camarasa, Cristina, de la Puente, Raul, Farga-Martí, Maria Amparo, Domínguez-Márquez, Victoria, Costa-Alcalde, José Javier, Trastoy, Rocío, Barbeito-Castiñeiras, Gema, Coira, Amparo, Pérez-del-Molino, María Luisa, Aguilera, Antonio, Planas, Anna M., Soriano, Alex, Fernandez-Cádenas, Israel, Pérez-Tur, Jordi, Marcos, Maria Ángeles, Moreno-Docón, Antonio, Viedma, Esther, Mingorance, Jesús, Galán-Montemayor, Juan Carlos, Parra-Grande, Mónica, Stadler, Tanja, Neher, Richard A., Carvajal Urueña, Ana María, Martín Sánchez, Vicente, Fregeneda Grandes, Juan Miguel, Molina de la Torre, Antonio José, Argüello Rodríguez, Héctor, Fernández Villa, Tania, Medicina Preventiva, Hodcroft, Emma B., Zuber, Moira, Nadeau, Sarah, Vaughan, Timothy G., Crawford, Katharine H. D., Althaus, Christian L., Reichmuth, Martina L., Bowen, John E., Walls, Alexandra C., Corti, Davide, Bloom, Jesse D., Veesler, David, Mateo, David, Hernando, Alberto, Comas, Iñaki, González-Candelas, Fernando, Goig, Galo Adrian, Chiner-Oms, Álvaro, Cancino-Muñoz, Irving, López, Mariana Gabriela, Torres-Puente, Manuela, Gomez-Navarro, Inmaculada, Jiménez-Serrano, Santiago, Ruiz-Roldán, Lidia, Bracho, María Alma, García-González, Neris, Martínez-Priego, Llúcia, Galán-Vendrell, Inmaculada, Ruiz-Hueso, Paula, De Marco, Griselda, Ferrús, Maria Loreto, Carbó-Ramírez, Sandra, D’Auria, Giuseppe, Coscollá, Mireia, Ruiz-Rodríguez, Paula, Roig-Sena, Francisco Javier, Sanmartín, Isabel, Garcia-Souto, Daniel, Pequeno-Valtierra, Ana, Tubio, Jose M. C., Rodríguez-Castro, Jorge, Rabella, Nuria, Navarro, Ferrán, Miró, Elisenda, Rodríguez-Iglesias, Manuel, Galán-Sanchez, Fátima, Rodriguez-Pallares, Salud, de Toro, María, Escudero, María Bea, Azcona-Gutiérrez, José Manuel, Alberdi, Miriam Blasco, Mayor, Alfredo, García-Basteiro, Alberto L., Moncunill, Gemma, Dobaño, Carlota, Cisteró, Pau, García-de-Viedma, Darío, Pérez-Lago, Laura, Herranz, Marta, Sicilia, Jon, Catalán-Alonso, Pilar, Muñoz, Patricia, Muñoz-Cuevas, Cristina, Rodríguez-Rodríguez, Guadalupe, Alberola-Enguidanos, Juan, Nogueira, Jose Miguel, Camarena, Juan José, Rezusta, Antonio, Tristancho-Baró, Alexander, Milagro, Ana, Martínez-Cameo, Nieves Felisa, Gracia-Grataloup, Yolanda, Martró, Elisa, Bordoy, Antoni E., Not, Anna, Antuori-Torres, Adrián, Benito, Rafael, Algarate, Sonia, Bueno, Jessica, del Pozo, Jose Luis, Boga, Jose Antonio, Castelló-Abietar, Cristián, Rojo-Alba, Susana, Alvarez-Argüelles, Marta Elena, Melon, Santiago, Aranzamendi-Zaldumbide, Maitane, Vergara-Gómez, Andrea, Fernández-Pinero, Jovita, Martínez, Miguel J., Vila, Jordi, Rubio, Elisa, Peiró-Mestres, Aida, Navero-Castillejos, Jessica, Posada, David, Valverde, Diana, Estévez-Gómez, Nuria, Fernandez-Silva, Iria, de Chiara, Loretta, Gallego-García, Pilar, Varela, Nair, Moreno, Rosario, Tirado, Maria Dolores, Gomez-Pinedo, Ulises, Gozalo-Margüello, Mónica, Eliecer-Cano, Maria, Méndez-Legaza, José Manuel, Rodríguez-Lozano, Jesus, Siller, María, Pablo-Marcos, Daniel, Oliver, Antonio, Reina, Jordi, López-Causapé, Carla, Canut-Blasco, Andrés, Hernáez-Crespo, Silvia, Cordón, Maria Luz A., Lecároz-Agara, María-Concepción, Gómez-González, Carmen, Aguirre-Quiñonero, Amaia, López-Mirones, José Israel, Fernández-Torres, Marina, Almela-Ferrer, Maria Rosario, Gonzalo-Jiménez, Nieves, Ruiz-García, Maria Montserrat, Galiana, Antonio, Sanchez-Almendro, Judith, Cilla, Gustavo, Montes, Milagrosa, Piñeiro, Luis, Sorarrain, Ane, Marimón, José María, Gomez-Ruiz, Maria Dolores, López-Hontangas, José Luis, González Barberá, Eva M., Navarro-Marí, José María, Pedrosa-Corral, Irene, Sanbonmatsu-Gámez, Sara, Pérez-González, Carmen, Chamizo-López, Francisco, Bordes-Benítez, Ana, Navarro, David, Albert, Eliseo, Torres, Ignacio, Gascón, Isabel, Torregrosa-Hetland, Cristina Juana, Pastor-Boix, Eva, Cascales-Ramos, Paloma, Fuster-Escrivá, Begoña, Gimeno-Cardona, Concepción, Ocete, María Dolores, Medina-Gonzalez, Rafael, González-Cantó, Julia, Martínez-Macias, Olalla, Palop-Borrás, Begoña, de Toro, Inmaculada, Mediavilla-Gradolph, Maria Concepción, Pérez-Ruiz, Mercedes, González-Recio, Óscar, Gutiérrez-Rivas, Mónica, Simarro-Córdoba, Encarnación, Lozano-Serra, Julia, Robles-Fonseca, Lorena, de Salazar, Adolfo, Viñuela-González, Laura, Chueca, Natalia, García, Federico, Gómez-Camarasa, Cristina, de la Puente, Raul, Farga-Martí, Maria Amparo, Domínguez-Márquez, Victoria, Costa-Alcalde, José Javier, Trastoy, Rocío, Barbeito-Castiñeiras, Gema, Coira, Amparo, Pérez-del-Molino, María Luisa, Aguilera, Antonio, Planas, Anna M., Soriano, Alex, Fernandez-Cádenas, Israel, Pérez-Tur, Jordi, Marcos, Maria Ángeles, Moreno-Docón, Antonio, Viedma, Esther, Mingorance, Jesús, Galán-Montemayor, Juan Carlos, Parra-Grande, Mónica, Stadler, Tanja, Neher, Richard A., Carvajal Urueña, Ana María, Martín Sánchez, Vicente, Fregeneda Grandes, Juan Miguel, Molina de la Torre, Antonio José, Argüello Rodríguez, Héctor, and Fernández Villa, Tania

Abstract

[EN] Following its emergence in late 2019, the spread of SARS-CoV-21,2 has been tracked by phylogenetic analysis of viral genome sequences in unprecedented detail3,4,5. Although the virus spread globally in early 2020 before borders closed, intercontinental travel has since been greatly reduced. However, travel within Europe resumed in the summer of 2020. Here we report on a SARS-CoV-2 variant, 20E (EU1), that was identified in Spain in early summer 2020 and subsequently spread across Europe. We find no evidence that this variant has increased transmissibility, but instead demonstrate how rising incidence in Spain, resumption of travel, and lack of effective screening and containment may explain the variant’s success. Despite travel restrictions, we estimate that 20E (EU1) was introduced hundreds of times to European countries by summertime travellers, which is likely to have undermined local efforts to minimize infection with SARS-CoV-2. Our results illustrate how a variant can rapidly become dominant even in the absence of a substantial transmission advantage in favourable epidemiological settings. Genomic surveillance is critical for understanding how travel can affect transmission of SARS-CoV-2, and thus for informing future containment strategies as travel resumes.

15. Frequent detection of monkeypox virus DNA in saliva, semen, and other clinical samples from 12 patients, Barcelona, Spain, May to June 2022.

Author

Peiró-Mestres A, Fuertes I, Camprubí-Ferrer D, Marcos MÁ, Vilella A, Navarro M, Rodriguez-Elena L, Riera J, Català A, Martínez MJ, and Blanco JL

Subjects
DNA, Viral genetics, Humans, Saliva, Semen, Spain epidemiology, Mpox, Monkeypox diagnosis, Mpox, Monkeypox epidemiology, Monkeypox virus isolation & purification
Abstract

A monkeypox (MPX) outbreak has expanded worldwide since May 2022. We tested 147 clinical samples collected at different time points from 12 patients by real-time PCR. MPX DNA was detected in saliva from all cases, sometimes with high viral loads. Other samples were frequently positive: rectal swab (11/12 cases), nasopharyngeal swab (10/12 cases), semen (7/9 cases), urine (9/12 cases) and faeces (8/12 cases). These results improve knowledge on virus shedding and the possible role of bodily fluids in disease transmission.

Published
2022
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