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1. Molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding a beta-xylosidase

Author

Perez-Gonzalez, Jose A., Peij, Noel N.M.E. van, Bezoen, Alja, MacCabe, Andrew P., Ramon, Daniel, and Graaff, Leo H. de

Subjects
Aspergillus -- Genetic aspects, Cloning -- Research, Genetic regulation -- Research, Biological sciences
Abstract

Research was conducted to examine the molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding the 85-kDA beta-xylosidase. The primary structure of the protein had considerable similarity to the primary structures of the Trichoderma reesei and Aspergillus niger beta-xylodases. Western blot analysis revealed elevated levels of production of the enzyme in overexpressing transformants showing that the cloned sequences existing in pXE1 encode the previously characterized 85-kDa beta-xylosidase.

Published
1998

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