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Abstract Background The World Health Organization (WHO) recommends that HIV treatment scale-up is accompanied by a robust assessment of drug resistance emergence and transmission. The WHO HIV Drug Resistance (HIVDR) monitoring and surveillance strategy includes HIVDR testing in adults both initiating and receiving antiretroviral therapy (ART). Due to limited information about HIVDR in Mozambique, we conducted two nationally representative surveys of adults initiating and receiving first-line ART regimes to better inform the HIV program. Methods We carried out a cross-sectional study between March 2017 and December 2019. Adults (older than 15 years) living with HIV (PLHIV) initiating ART or receiving first-line ART for between 9-15 months at 25 health facilities across all eleven provinces in Mozambique were included. Genotypic HIVDR was assessed on dried blood spots (DBS) when viral loads were ≥ 1000 copies/ml. Genotypic resistance for non-nucleoside reverse transcriptase inhibitors (NNRTIs), nucleoside reverse transcriptase inhibitors (NRTIs), and protease inhibitors (PIs) was determined using the Stanford HIV database algorithm 9.5 and calibrated population resistance tool 8.1. Results Of 828 participants -enrolled, viral load (VL) testing was performed on 408 initiators and 409 ART experienced. Unsuppressed VL was found in 68.1% 419 initiators and 18.8% (77/409) of the ART experienced. Of the 278 initiators and 70 ART experienced who underwent sequencing, 51.7% (144/278) and 75.7% (53/70) were sequenced successfully. Among the new initiators, pretreatment drug resistance (PDR) for NNRTI and PI was found in 16.0% (23/144) and 1.4% (2/144) of the participants, respectively. Acquired drug resistance (ADR) was found in 56.5% (30/53) of the ART-experienced participants of whom 24.5% (13/53) were resistant to both NRTI and NNRTI. Conclusion High rates of PDR and ADR for NNRTI and ADR for NRTI were observed in our study. These findings support the replacement of NNRTIs with dolutegravir (DTG) but high levels of NRTI resistance in highly treatment-experienced individuals still require attention when transitioning to new regimens. Moreover, the study underlines the need for routine VL testing and HIVDR surveillance to improve treatment management strategies.

Este artigo debruça-se sobre o discurso de condolências por parte de utilizadores em obituários publicados por funerárias locais portuguesas no Facebook. Com o objetivo de compreender o discurso de luto e identificar dinâmicas comunitárias online, que dialogam com a comunidade física em que a funerária local se insere, optou-se por uma metodologia que alia princípios da análise crítica do discurso e da etnografia. Desta análise resultaram diversas considerações: revelaram-se espaços de elevada informalidade, visível ao nível da linguagem através da disseminação de erros gramaticais, pela utilização de elementos pictográficos (e.g., emojis), ou pela recorrência de utilizadores que produzem idênticos comentários nas publicações, independentemente de quem morre; motivaram contrastes discursivos, desde interações que poderão ser consideradas despropositadas até comentários que partilham experiências de luto; são espaços com potencial para lembrar, caracterizar e imaginar os mortos, através de relatos de histórias que o utilizador experienciou com a pessoa. É também argumentado que esta imensa diversidade é incentivada pela eficácia do Facebook em se integrar no quotidiano das pessoas e pelo seu caráter mediado que, incentivado pelo que parece ser uma carência de literacia emocional em contexto digital, parece desresponsabilizar certos utilizadores no discurso produzido.

Resulta esencial la integración del enfoque Ciencia-Tecnología-Sociedad en el desempeño profesional del profesorado universitario, sin embargo, se evidencian limitaciones que justifican el problema científico referido a: insuficiencias en la evaluación del impacto de las acciones de superación profesional del profesorado universitario en el enfoque Ciencia-Tecnología-Sociedad. El objetivo del artículo es proponer una metodología de evaluación de impacto de la superación del profesorado universitario en el enfoque Ciencia-Tecnología- Sociedad.Se utilizaron métodos como: grupo de discusión y cuestionario a egresados de maestríasy diplomados, análisis de contenido a tesis doctorales y trabajos académicos, así como entrevistas a vicedecanos, metodólogos y coordinadores de programas de posgrado. Se aportó una metodología de evaluación de impacto con procedimientos de organización, ejecución,interpretación de la información y socialización. La valoración cuantitativa y cualitativa de los indicadores posibilitó la evaluación del impacto de las acciones en un nivel alto y la integración de propuestas de mejora.

Abstract Background Vision depends on the interplay between photoreceptor cells of the neural retina and the underlying retinal pigment epithelium (RPE). Most genes involved in inherited retinal diseases display specific spatiotemporal expression within these interconnected retinal components through the local recruitment of cis-regulatory elements (CREs) in 3D nuclear space. Results To understand the role of differential chromatin architecture in establishing tissue-specific expression at inherited retinal disease loci, we mapped genome-wide chromatin interactions using in situ Hi-C and H3K4me3 HiChIP on neural retina and RPE/choroid from human adult donor eyes. We observed chromatin looping between active promoters and 32,425 and 8060 candidate CREs in the neural retina and RPE/choroid, respectively. A comparative 3D genome analysis between these two retinal tissues revealed that 56% of 290 known inherited retinal disease genes were marked by differential chromatin interactions. One of these was ABCA4, which is implicated in the most common autosomal recessive inherited retinal disease. We zoomed in on retina- and RPE-specific cis-regulatory interactions at the ABCA4 locus using high-resolution UMI-4C. Integration with bulk and single-cell epigenomic datasets and in vivo enhancer assays in zebrafish revealed tissue-specific CREs interacting with ABCA4. Conclusions Through comparative 3D genome mapping, based on genome-wide, promoter-centric, and locus-specific assays of human neural retina and RPE, we have shown that gene regulation at key inherited retinal disease loci is likely mediated by tissue-specific chromatin interactions. These findings do not only provide insight into tissue-specific regulatory landscapes at retinal disease loci, but also delineate the search space for non-coding genomic variation underlying unsolved inherited retinal diseases. Graphical Abstract

Abstract Background Orbital metastasis is a possible complication of small cell lung cancer and a pattern of bilateral invasion of the extraocular muscles has rarely been reported in literature. Case presentation A 46-year-old white male with a past medical history of smoking and stage IV small cell lung carcinoma presented with loss of vision and pain in the left eye. Examination revealed bilateral proptosis and left afferent pupillary defect, and visual acuity was hand motion on the left eye and 4/10 on the right eye. An orbital computed tomography scan showed a compression of the left optic nerve between the extraocular muscles at the apex, and a lateral canthotomy was performed for a new-onset compressive optic neuropathy, with residual visual improvement. There was also significant enlargement of the extraocular muscles in the right orbit. The patient was maintained in palliative treatment with both chemotherapy and local medical and surgical (amniotic membrane cover for exposure keratopathy) ophthalmological treatments until he eventually died 5 months after. Conclusion Bilateral metastasis to the extraocular muscles is a very rare manifestation of small cell lung cancer and the palliative treatment in these cases is challenging.

Se caracteriza la infraestructura hidráulica de esta empresa, identificando problemáticas que afectan su productividad y uso eficiente del agua de riego, a partir de entrevistas a directivos y personal técnico, para establecer criterios de selección de técnicas de riego en áreas potencialmente agrícolas en secano. Del levantamiento realizado en la infraestructura de riego y drenaje, fondo de tierra de unidades productivas y análisis del complejo Agua, Suelo, Planta, el 58.6 % de las máquinas de Pivote Central están en explotación y se aprovecha, menos del 50 % del agua para el riego, por pérdidas por infiltración y otras causas, en los canales que conducen el agua. De la superficie agrícola, el 35 % del área está bajo riego y las instalaciones funcionan sin previa evaluación de los parámetros de manejo.

Representation based on the visualization of reality, acquired through active and passive sensors, has allowed a rethinking of the traditional cognitive and communicative process. The Vitruvian approach has been replaced by the discrete visualization of large number of point-images. Image- or range-based coordinates allow to overcome the limitations of the drawing or photographic image, configuring a 3D model that can be explored in any direction and detail, even remotely. Automatic and semi-automatic procedures manage the information to be conveyed to the numerical control of the models.Many scientific papers deal with the certified reliability of the data or the optimization of the models; several papers discuss the development of programmed instructions to automate the “intelligent” recognition of components in Building Information Modeling (BIM) and Heritage Building Information Modeling (H-BIM) workflows. However, few discussions focus on the heuristic value of using time-saving procedures. The phases of acquisition, processing, critical synthesis, and communication of results are now included in a single workflow centered on the use of Information and Communication Technologies (ICT) to capture and transfer the archived information framework. This unified process has affected research and teaching methods, as well as the way in which information is disseminated and citizens are involved, in order to obtain the approval and thus the resources needed for the political management of heritage.Starting from these considerations, it seemed appropriate to recontextualize a process that is not limited to the improvement of fast and simplified operations, as an alternative to calculations congruent with the purpose of the project or survey. Indeed, the call for this issue was intended to propose and encourage a broader reflection of current research trends and to stimulate discussion on the limits and potentials of advanced automation processes in relation to the comparison of established and innovative practices.The awareness gained from digital language shows the widespread reconsideration of geometric logic. A trend that can be clearly seen in the papers dealing with the digital representation of complex vaulted systems or design solutions based on the reinterpretation of the Vitruvian concept of scenography. Less obvious, but no less diriment for this reason, are the geometric considerations that support quad-dominant remeshing or retopology solutions, according to the lexicon of computer graphics.Refined geometric solutions to improve analysis and details applicable to “reliable copies” of the virtually reconstructed object to control the change of state in the physical duplicate can also be observed in reverse modeling techniques.In parallel, the evolution of digital language with services provided by the network enhances the physical experience, improving it with the help of virtual content to guide meaningful experiences. In this dual perspective, the title of the volume alludes to the path, cyclical and interactive, that unites, without confusing, the construction of image- and range-based, in order to use them as spaces of collaboration suitable for the active dissemination of knowledge.DOI: https://doi.org/10.20365/disegnarecon.32.2024.ed

Introduction: Conjunctival cysts of the orbit development are a rare complication in enucleated patients. They result from the entrapment of conjunctival epithelium within the orbit. Case Presentation: We report the case of a 25-year-old man with a history of enucleation for a retinoblastoma of the right eye who presented with difficulty in fitting his eye prothesis. On his past medical records, there was no reference to the placement of any orbital implant at the time of the surgery. Biomicroscopy of the right eye revealed a thickened bulbar conjunctiva, an inferior symblepharon, and a translucid central area with vascularization. Imaging was remarkable for a cystic cavity filling the whole right orbit. Biopsy revealed the diagnosis of a conjunctival cyst, and drainage was performed, alleviating the patient’s symptoms. Conclusion: Orbital conjunctival cysts may pose a clinical problem, and treatment modalities include surgical excision, absolute alcohol injection, and trichloroacetic acid injection.

Every architectural drawing is by its nature the result of a combined operation of projection lines and intersection with a plane, which is usually a sheet of paper. Even a sketch, in its rarefied figuration, evokes such operations for a representation of a design idea or an executive work, in an unequivocal icasticity. [read more]

Secondary forests can play a critical role in conserving plant biodiversity and sequestering carbon. However, intensive past management, fragmentation, and initial soil fertility can hinder forest recovery during secondary succession, especially in terms of tree species selection. In turn, tree species selection can negatively impact soil fertility recovery and may slow down the succession process, but the direct and indirect effects between these factors remain unclear. To assess the complex interactions among landscape, previous management, soil and vegetation, 27 secondary forests of varying ages, previously used for eucalyptus plantations, located in diverse landscape configurations within the Atlantic Forest biome, were examined. Key variables, such as planting frequency, cutting frequency, forest cover in the landscape, patch isolation between fragments, functional richness, tree aboveground biomass (AGB), soil sum of bases, soil organic matter, and soil phosphorus concentration were used to construct a structural equation model to evaluate the direct and indirect effects of landscape and previous management on forest development. Cutting frequency and patch isolation had a negative direct effect on functional richness. Additionally, a strong positive direct effect of functional diversity on aboveground biomass and soil sum of bases was found. Thus, cutting frequency and patch isolation had negative indirect effects on biomass and soil sum of bases (a proxy for soil fertility), mediated by functional richness. These findings underscore the significance of integrating plant functional diversity into restoration strategies to preserve ecosystem functioning and efficiently recover biodiversity, tree biomass, and soil fertility in secondary forests.

Nos proponemos estudiar la inscripción latina que la Junta General del Principado de Asturias dedicó en Oviedo, en 1798, a Gaspar Melchor de Jovellanos, con ocasión de su nombramiento como ministro de Gracia y Justicia. Ofrecemos una nueva edición crítica del texto y un análisis de sus aspectos lingüísticos más relevantes; también proponemos una nueva traducción más ajustada al texto latino que la única que conocemos; esta última se caracteriza por una majestuosidad y elegancia de las que, en absoluto, carece el texto original, como se desprende del análisis lingüístico. Por último, hacemos unas consideraciones sobre la autoría del texto.

Point clouds are an essential tool for documenting archaeological and architectural heritage, since they are the initial product that can be obtained from a laser scanner or photogrammetry survey. Despite being a very basic and discretized source of information containing only coloured points, they can be used as a first approach to the surveyed model through a virtual visualization or interactive tour.The management and visualization of point clouds is not an easy task, since it involves the representation of millions of points and installing specific viewers is mandatory. These special viewers are developed by each laser scanner manufacturer to visualize its own point cloud file format. Handling with them can result unfriendly and non-intuitive for a non-specialized public. Therefore, the main objective of this work is to develop a working methodology based on Unreal Engine, a video game development environment, to create self-executing visualization modules that can be shared with any user. These modules will allow simple and fluid navigation of point clouds without the need of installing any additional software or having specific knowledge.These self-executing modules will allow any kind of viewer, specialised or not, to visualize the point cloud in a first-person way or from a bird’s eye view. The exposed methodology has been tested using a survey of the Oliva Castle in Valencia (Spain), which is classified as a cultural interest asset. In order to illustrate the results of this research, some images of the visualization environment will be shown and the download link of the display module corresponding to the Oliva Castle will be provided.DOI: https://doi.org/10.20365/disegnarecon.30.2023.13

The challenge of MSME business is the change of transaction systems from conventional to digital processes to minimize human error. This research offers an idea to identify problems, classify digital innovation ideas, analyze needs, and design management information systems Point of Sales (POS) modules in CV. Renaldi Motor. The method used in designing the management information system of the POS module of the OmO Jaya Workshop application is design thinking. The stages in this research consist of empathizing, defining, ideating, prototyping, and testing. The results of this study show that the problem faced by Micro, Small, and Medium Enterprises (MSMEs) workshop businesses is the process of recording transactions that still use conventional methods. Through the digital innovation of the POS module management information system in the form of the OmO Jaya workshop application. Through the application, a CV. Renaldi Motor is expected to optimize the digital transaction recording system and improve business performance. Thus, the risk of business losses due to human error can be minimized. In addition, the Blackbox test results show that the test results in each process have been successful and as expected. This study concluded that the Point of Sales (POS) System can improve CV. Renaldi Motor's business performance through digitizing the sales transaction recording process.

El presente estudio, establece el marco teórico-científico para la futura implementación de sistemas de geolocalización en redes LPWAN, con énfasis fundamental en la localización georreferencial de dispositivos IoT que utilizan la tecnología LoRaWAN. El mismo se enfocará en la exposición teórica de los principios esenciales que deben considerarse para la instauración de sistemas de geolocalización en entornos de dos dimensiones, realizando una breve descripción de los parámetros físicos principales que caracterizan a las señales de radio en estos entornos de trabajo. A la vez que se resaltan las principales técnicas que se emplean, se abordan los algoritmos de estimación de posición más conocidos y empleados en la actualidad, así como sus principios de funcionamiento. De esta manera, se pretende establecer un contexto teórico para la explicación de los diferentes métodos de posicionamiento que permiten una correcta implementación de estos algoritmos. Además se utiliza como un sondeo de las diversas tecnologías inalámbricas que permiten su implementación en sistemas de geolocalización en interior y exterior. En síntesis, se sientan los cimientos teórico-científicos sobre los fundamentos, algoritmos, métodos y tecnologías claves a considerar en el desarrollo de sistemas de geolocalización eficientes, seguros y de bajo consumo de energía tan necesarios en un mundo cada vez más interconectado.

Introducción: la comunicación por parte del logofonoaudiólogo con el paciente afásico y su familia garantiza el bienestar biopsicosocial de estos y favorece una adecuada relación médico-paciente. Objetivo: evaluar una propuesta de estrategia para el fomento del principio de beneficencia entre el logofonoaudiólogo, el paciente afásico y su familia en el Policlínico Universitario Dr. René Vallejo Ortiz, ciudad de Manzanillo, Granma, Cuba. Método: fue aplicada la técnica de Iadov o criterio de usuario que permitió evaluar la propuesta. Para ello se encuestaron 15 logofonoaudiólogos de la provincia de Granma, Cuba, se escogieron de manera intencional 10, cuyas cualidades cumplían los requisitos exigidos para ser considerados usuarios. Se utilizaron diferentes métodos, técnicas y procedimientos para procesar y analizar los datos recogidos. La escala valorativa Likert facilitó la interpretación cualitativa de los datos recogidos. Resultados: desde el criterio individual el 60 % de los usuarios consideró la propuesta bastante adecuada; la valoración más baja fue de adecuada (20 %). El cálculo de la valoración grupal, el índice obtenido (0,5 %) le confirió confiabilidad a la propuesta para ser implementada. Conclusiones: los usuarios consideran que la propuesta de estrategia cumple con los requisitos para la aplicación. No obstante, existen algunos elementos que pudieran dificultar la ejecución de la misma: la incorrecta preparación científico-metodológica de los facilitadores, el uso inadecuado de los métodos y técnicas educativas, así como la negativa de aquellos logofonoaudiólogos que no concienticen la necesidad de alcanzar modos de actuación acorde a los principios y valores profesionales.

Background: Latin America has registered cases in children under 14 years of age, which seem few compared to older age groups but are relevant since the child and youth population have been indirectly receiving the most decisive impact of the pandemic. Ensuring the most significant protection for this age group is essential. Methods: This is an ecological study of 10 Latin American and the Caribbean countries to study protecting children and adolescents during the COVID-19 pandemic. Using the information from UNICEF, a coverage analysis was done, and a new variable score was proposed. Also, a multiple linear regression model was tailored to evaluate the relationship between confirmed cases by 100k of COVID-19 (0–14 year population) and the performance of the countries and some key indicators. Results: A strong and statistically significant correlation (r = 0.79; p value 0.005) between the increased coverage of child helplines and a greater number of school closures. Relationship of confirmed cases by COVID-19 and stringency index with a coef B = −2163.6, [CI −3122.1; −1205.1]; p value = 0.010. Cases and healthcare access with a coef B = −17459.7, [CI −25630.1; −9289.5]. Conclusions: Experts say other pandemics will come, and this emergency must be turned into an opportunity. It is vital to strengthen protection programs for the population, especially vulnerable people, such as children and young people. Resumo

Considering performances with animals such as Going to the Dogs (Wim T. Schippers, 1986), Octavio and I (Linda Molenaar, 2010), Performances for Pets (Juurak/ Bailey 2014) and A Performance With an Ocean View (for a Dog) (Kokkonnen, 2008), I argue that these works illustrate three emergent effects of performing with animals: challenging a binary and exclusionary view of co-presence in performance; determining a modification of the performer’s interaction with a non- human animal audience; reversing the humanization of the animal, by rehearsing an animalization of human agency.

Long-term monitoring data is central for the analysis of biodiversity change and its drivers. Time series allow a more accurate evaluation of diversity indices, trait identification and community turnover. However, evaluating data collected across different monitoring programs remains complicated because of data discrepancies and inconsistencies. Here we propose a method for aggregating datasets using diffusion maps. The method is illustrated by aggregating long-term phytoplankton abundance data from the Wadden Sea and Southern North Sea gathered by two institutions located in Germany and The Netherlands. The aggregated data allowed us to infer species traits, to reconstruct the main trait axis which drives community functionality, ultimately quantifying functional diversity of the individual samples, having used only the co-occurrence of species in samples. Although functional diversity varies greatly among sampling stations, we detect a slight positive trend in German stations, which contrasts with the clear decreasing trend observed in most of the Dutch Wadden Sea stations. At the Terschelling transect, in Southern North Sea, the stations also showed contrasting estimations of functional diversity between off-shore and in-shore stations. Our research provides further evidence that traits and functional diversity can be robustly reconstructed from monitoring data alone, showing that data aggregation can increase the accuracy of this reconstruction, being able to aggregate heterogeneous datasets.

Abstract Introduction: Acute kidney injury (AKI) occurs frequently in COVID-19 patients and is associated with greater morbidity and mortality. Knowing the risks of AKI allows for identification, prevention, and timely treatment. This study aimed to identify the risk factors associated with AKI in hospitalized patients. Methods: A descriptive, retrospective, cross-sectional, and analytical component study of adult patients hospitalized with COVID-19 from March 1 to December 31, 2020 was carried out. AKI was defined by the creatinine criteria of the KDIGO-AKI guidelines. Information, regarding risk factors, was obtained from electronic medical records. Results: Out of the 934 patients, 42.93% developed AKI, 60.59% KDIGO-1, and 9.9% required renal replacement therapy. Patients with AKI had longer hospital stay, higher mortality, and required more intensive care unit (ICU) admission, mechanical ventilation, and vasopressor support. Multivariate analysis showed that age (OR 1.03; 95% CI 1.02–1.04), male sex (OR 2.13; 95% CI 1.49–3.04), diabetes mellitus (DM) (OR 1.55; 95% CI 1.04–2.32), chronic kidney disease (CKD) (OR 2.07; 95% CI 1.06–4.04), C-reactive protein (CRP) (OR 1.02; 95% CI 1.00–1.03), ICU admission (OR 1.81; 95% CI 1.04–3.16), and vasopressor support (OR 7.46; 95% CI 3.34–16.64) were risk factors for AKI, and that bicarbonate (OR 0.89; 95% CI 0.84–0.94) and partial pressure arterial oxygen/inspired oxygen fraction index (OR 0.99; 95% CI 0.98–0.99) could be protective factors. Conclusions: A high frequency of AKI was documented in COVID-19 patients, with several predictors: age, male sex, DM, CKD, CRP, ICU admission, and vasopressor support. AKI occurred more frequently in patients with higher disease severity and was associated with higher mortality and worse outcomes.

En el contexto de la Licenciatura Rehabilitación en Salud se presentan insuficiencias que limitan la calidad del proceso formativo, evidenciadas en el insuficiente desempeño docente asistencial del profesorado. Se plantea como problema de investigación: cómo contribuir al desempeño docente asistencial del profesorado de la carrera Rehabilitación en Salud de la Facultad de Ciencias Médicas de Morón. Por ello, se propone como objetivo de la investigación: elaborar un Programa de Orientación Profesional sustentado en un Modelo de la Dinámica del Proceso de Superación Profesional que contribuya al desempeño docente asistencial del profesorado de la carrera Rehabilitación en Salud. Se emplearon como parte de la metodología el análisis de documentos, la observación, entrevistas y encuestas. La valoración de los resultados de la implementación de este proceder corroboró su pertinencia científico-metodológica.

La presente publicación es el resultado de una investigación sobre los sistemas de gestión por procesos aplicados a organizaciones empresariales para mejorar la desorganización en sus operaciones y que básicamente están vinculada con la duplicidad de actividades, o falta de gestión para contactar con un cliente para la venta o renovación de productos o servicios, lo que influye directamente en los ingresos de la organización y por consiguiente afecta la rentabilidad del negocio, su productividad y competitividad. Con un enfoque exploratorio y descriptivo se revisó parte de la población disponible bibliográfica sobre sistemas de gestión por proceso, de los cuales se tomaron los aspectos más relevantes según los autores del proyecto de investigación, aunado a la experiencia profesional sobre manejo de estos sistemas de gestión por procesos, y se logró plantear un diseño propio de un modelo de sistema de gestión por procesos. En la aplicación reiterada del mismo se irá validando. Palabras clave: Gestión, organizaciones, procesos. ABSTRACT This publication is the result of research on process management systems applied to business organizations to improve disorganization in their operations and that are basically linked to the duplication of activities, or lack of management to contact a customer for sale. or renewal of products or services, which directly influences the income of the organization and therefore affects the profitability of the business, its productivity and competitiveness. With an exploratory and descriptive approach, part of the available bibliographic population on management systems by process was reviewed, from which the most relevant aspects were taken according to the authors of the research project, together with the professional experience in managing these management systems. by processes, and it was possible to propose an own design of a management system model by processes. In the repeated application of the same, it will be validated. Keywords: Management, organizations, processes.

Encapsulating peritoneal sclerosis (EPS) is a rare complication of peritoneal dialysis which mortality can reach 50%. The usual treatment involves suspension of the technique, medical treatment, and surgical enterolysis. We report the case of a 36-year-old female who undergone continuous ambulatory peritoneal dialysis without complications for 10 years. She presented at the emergency department with abdominal pain and a diagnosis of peritonitis was assumed. The patient developed a systemic inflammatory response, and her abdominal computer tomography (CT) scan showed multiple parietal calcifications. After multiple broad-spectrum antibiotics, an undetermined febrile syndrome persisted, and parenteral nutrition was started. An EPS diagnosis was established and treatment with tamoxifen was proposed. Exploratory laparoscopy was decided, and the intraoperative findings were consistent with EPS. The patient was submitted to extensive enterolysis. A clinical improvement led to a discharge after 60 days of hospitalization. After more than 30 months with tamoxifen, she remains on hemodialysis and doing well. There are no randomized controlled trials to guide EPS management and the following procedures are more difficult to decide, who makes these reports important to the reflection. We describe a case of successful treatment of EPS, with a very severe presentation. The management with enterolysis and tamoxifen contributed decisively to this outcome.

The objective of this study was to describe phenotypically and biochemically the symbiotic bacteria associated with three populations of Heterorhabditis amazonensis Andalo et al., 2006 (isolates: UEL-n 01, UEL-n 07, and UEL-n 08) and evaluate their pathogenicity on Galleria mellonella L. (Lepidoptera: Pyralidae) larvae. Bacteria were isolated by maceration of infective juveniles (IJs) and grown in culture medium (NBTA and MacConkey). The characterization of the bacteria was evaluated by employing motility test and biochemical tests like Gram staining, lipase activity, protease, and lecithinase. The production of antibiotics and bioluminescence was also evaluated. The pathogenicity was evaluated on the last instar larvae of G. mellonella at a concentration of 104 cells/mL. The bacteria from the three entomopathogenic nematodes isolates were positive for all biochemical tests except for lecithinase, and have presented bioluminescence when subjected to ultraviolet light, indicating that they belong to the genus Photorhabdus sp. Both were pathogenic to G. mellonella larvae causing 93.3 to 100.0% mortality.

Introducción: Se realizó una revisión que permite comprender mejor por qué es necesaria una visión más integradora de la neuropsicología del adolescente para la aplicación de métodos de intervención desde las neurociencias que permitan enfrentar la conducta de riesgo sexual y reproductivo. Objetivo: Actualizar sobre los aspectos particulares de la neurofisiología de la adolescencia, que los puede hacer proclives a desarrollar una conducta de riesgo en cuanto a la función reproductiva. Material y Método: Revisión de la literatura sobre el tema, publicada en el período 2017-2022. Se utilizaron las bases de datos: PubMed/MEDLINE, Lilacs, Scielo, Cochrane, y The National Library of Medicine. Resultaron incluidas 26 fuentes bibliográficas para las citas, por reunir los requisitos de mayor especificidad, originalidad y aporte al tema. Desarrollo: El desarrollo del adolescente genera transformaciones radicales en su personalidad, que están relacionadas con cambios importantes en las estructuras cerebrales, que aún no están completamente desarrolladas. Las técnicas de resonancia magnética muestran que los principales cambios en el cerebro del adolescente afectan la corteza prefrontal, por lo que son más proclives a la falta de autorregulación y a una excesiva excitabilidad. La plasticidad de esta etapa explicaría cómo las conductas erróneas pueden imprimirse negativamente y dar lugar a las actitudes que llevan al fracaso, particularmente aquellas relacionadas con la sexualidad y la función reproductiva. Conclusiones: Se aportaron reflexiones críticas que permiten confirmar el hecho de que existen insuficiencias en los métodos para la modificación de la conducta de riesgo sexual y la función reproductiva en adolescentes. Se aportan nuevos enfoques de enfrentamiento.

Cercarial dermatitis (CD), or “Swimmer’s itch” as it is also known, is a waterborne illness caused by a blood fluke from the family Schistosomatidae. It occurs when cercariae of trematode species that do not have humans as their definitive host accidentally penetrate human skin (in an aquatic environment) and trigger allergic symptoms at the site of contact. It is an emerging zoonosis that occurs through water and is often overlooked during differential diagnosis. Some of the factors contributing to the emergence of diseases like CD are related to global warming, which brings about climate change, water eutrophication, the colonization of ponds by snails susceptible to the parasite, and sunlight exposure in the summer, associated with migratory bird routes. Therefore, with the increase in tourism, especially at fluvial beaches, it is relevant to analyze the current epidemiological scenario of CD in European countries and the potential regions at risk.

Chromatic Pupillometry, used to assess Pupil Light Reflex (PLR) to a coloured light stimulus, has regained interest since the discovery of melanopsin in the intrinsically photosensitive Retinal Ganglion Cells (ipRGCs). This technique has shown the potential to be used as a screening tool for neuro-ophthalmological diseases; however, most of the pupillometers available are expensive and not portable, making it harder for them to be used as a widespread screening tool. In this study, we developed a smartphone-based system for chromatic pupillometry that allows targeted stimulation of the ipRGCs. Using a smartphone, this system is portable and accessible and takes advantage of the location of the ipRGCs in the perifovea. The system incorporates a 3D-printed support for the smartphone and an illumination system. Preliminary tests were carried out on a single individual and then validated on eleven healthy individuals with two different LED intensities. The average Post-Illumination Pupil Light Response 6 s after the stimuli offsets (PIPR-6s) showed a difference between the blue and the red stimuli of 9.5% for both intensities, which aligns with the studies using full-field stimulators. The results validated this system for a targeted stimulation of the ipRGCs for chromatic pupillometry, with the potential to be a portable and accessible screening tool for neuro-ophthalmological diseases.

Carditis in childhood is a rare disease with several etiologies. We report a case of infant death due to pericarditis and myocarditis after the mRNA vaccine against COVID-19 (COVIDmRNAV). A 7-year-old male child received the first dose of the COVIDmRNAV and presented with monoarthritis and a fever non-responsive to oral antibiotics. The laboratory investigation showed signs of infection (leukocytosis, high levels of c-reactive protein). His condition rapidly deteriorated, and the patient died. The autopsy identified pericardial fibrin deposits, hemorrhagic areas in the myocardium, and normal valves. A diffuse intermyocardial inflammatory infiltrate composed of T CD8+ lymphocytes and histiocytes was identified. An antistreptolysin O (ASO) dosage showed high titers. The presence of arthritis, elevated ASO, and carditis fulfills the criteria for rheumatic fever. However, valve disease and Aschoff’s nodules, present in 90% of rheumatic carditis cases, were absent in this case. The temporal correlation with mRNA vaccination prompted its inclusion as one of the etiologies. In cases of myocardial damage related to COVID-19mRNAV, it appears to be related to the expression of exosomes and lipid nanoparticles, leading to a cytokine storm. The potential effects of the COVID-19mRNAV must be considered in the pathogenesis of this disease, whether as an etiology or a contributing factor to a previously initiated myocardial injury.

(1) Background: Psychological wellbeing correlates with improved physical and psychological health, as this construct plays a fundamental role in disease recovery and health maintenance. Hence, for healthcare professionals, understanding the factors that predict psychological wellbeing is of great interest. Thus, the objective of this study was to determine whether health status and personality traits influence psychological wellbeing. (2) Methods: The total sample (N = 600) consisted of HIV patients, individuals with diabetes, and healthy subjects from the Salamanca Clinical Hospital. The instruments used for data collection included a sociodemographic questionnaire, Ryff’s Psychological Wellbeing Scale, and the Spanish version of the Big Five Taxonomy to measure personality. (3) Results: Specific personality traits, such as Emotional Stability, Extraversion, Responsibility, and Integrity were significant predictors of different dimensions of psychological wellbeing. Regarding health status, individuals with diabetes and healthy subjects, compared to HIV+ subjects, were associated with higher levels of psychological wellbeing dimensions. (4) Conclusions: Individual differences in personality traits and the diagnosis of a chronic condition may play a fundamental role in psychological wellbeing. These conclusions are of great interest for developing strategies aimed at individuals with chronic illnesses and specific personality traits associated with poorer psychological wellbeing.

Background: The COVID-19 pandemic may affect youth’s physical and mental well-being, partially because of the countries’ rules to contain the virus from spreading. However, there is still uncertainty about the impact of physical distancing on youth’s mental health. We aimed to estimate the prevalence of feeling agitated, anxious, down, sad, or low mood (FNF) due to physical distance measures and verify which factors are associated with young Portuguese and Brazilian people. Methods: We used cross-sectional data from the instrument “COVID-19 Barometer: Social Opinion” in Portugal (March 2020 and September 2021) and from “COVID-19 Social Thermometer” in Brazil (August 2020 to April 2021); these surveys included data regarding the health and socioeconomic impact on the population. The health and sociodemographic variables of the two countries were summarized as absolute and relative frequencies. A multivariable logistic regression model was fit by country to estimate the relation between FNF and selected variables of interest. Results: Approximately 36% of the sample studied reported anxiety, agitation, sadness, or low mood almost every day in Portugal and 52% in Brazil due to physical distancing. In Portugal, having more than two comorbidities represented a greater chance of experiencing FNF every day or almost every day (odds ratio [OR] = 1.51 [CI: 1.22–1.87]) than those without comorbidities. In contrast, having a university education level represented a protector factor (OR = 0.76 [CI: 0.67–0.88]). In Brazil, being unemployed increased the chance of FNF compared to students (OR = 11.2). Conclusions: Physical distancing measures have impacted the mental well-being of the young population in Portugal and Brazil. The countries must make a quick effort to attend to and protect young people’s well-being and mental health in the changing context of the current pandemic.

En el presente trabajo se presenta una síntesis y discusión sobre la presencia de patologías congénitas y defectos del desarrollo en individuos de diferentes periodos arqueológicos de la costa de la región de Antofagasta, en el norte de Chile, las que se concentran en el esqueleto axial. A pesar de ser un número bajo (n: 6), se realiza una comparación con aquellas reportadas para la zona costera de Arica y Tarapacá, obteniéndose que las diferencia entre ambas zonas no es estadísticamente significativa. Considerando antecedentes ambientales y etnohistóricos de la zona, se propone que una combinación a la exposición de diferentes contaminantes naturales, junto a un sistema de parentesco endogámico serían las causas más probables de la aparición de estas patologías.

Abstract Background The variable visual function observed in diabetic retinopathy (DR) patients is not fully explained by the classic staging system. Our purpose was to evaluate choroidal changes, in standardized sectors, in DR patients and to find associations between choroidal measurements and visual function. Methods Cross-sectional study that included the right eye of diabetic patients (n = 265) without active edema, ischemia or neovascularization and age-matched controls (n = 73). Optical coherence tomography (OCT) imaging was performed with enhanced depth imaging protocol. Choroidal vascularity index (CVI) was calculated in a 5 mm scan centered in the fovea. Results CVI decreased with age (p 0.066). Conclusions A reduction in CVI was observed in patients with a more advanced stage of DR. In treated DR patients with stable disease, choroidal biomarkers correlated with best-corrected visual acuity whereas retinal biomarkers did not. Trial registration: N/A

Abstract Background Predicting how natural and anthropogenic drivers shape different ecological indicators, such as plant populations along environmental gradients, can be a relevant tool for establishing management and conservation criteria of tropical wetlands. We aimed to assess the effects of seasonal flood disturbance, type of grasslands and topographical conditions on Croton trinitatis population distribution in a tropical wetland. Methods The study was carried out in a seasonally flooded grassland (Central-West Brazil). We conducted samplings of soil on the dry and flood hydrophases of the Pantanal. We took the samples in eight seasonal ponds, with 1 km interval between them. Transects were marked during the flood period, observing the water level, one in the lowest zone, in the middle of the pond = low (ca. 60 cm deep), one at the pond edge = mid (ca. 30 cm deep) and one in the higher zone, on the external part = high (ca. 1 cm deep). Results The results showed that the topography, seasonality, and types of grassland determine differences in the abundance patterns of adult plants and seedlings, and seed bank and seed predation. The abiotic factors can shape plant population-related ecological processes and patterns, with outputs (germination and predation) and inputs (local dispersion and from neighbouring areas) of proportional seeds for the population maintenance in this environment. Conclusion We emphasize the importance of these findings, to show that abiotic factors are not the only ones to be considered in ecological studies of distribution and structuring of populations in habitats with extreme seasonal events.

Introduction: Platypnea-orthodeoxia syndrome (POS) is a rare disorder characterized by dyspnoea and oxygen desaturation in the upright position which improves in the supine position. Clinical case: 84 years old woman with hypertension and Parkinson disease. She came to the emergency department complaining of dyspnoea. At admission she was polypneic (35 bpm), spO2 80%, 157/108mmHg, 115bpm, apyretic, normal pulmonary auscultation and without peripheral oedema. Oxygen supplementation was started with lack of response. The patient, which at first was lying in the bed with the headboard at 45º, recovered the peripheral oxygen saturation to 96-97% when the bed was placed at 0º. Diagnostic tests were performed, excluding cardiac myocardial infarction, pulmonary embolism and pneumonia. Transthoracic echocardiogram revealed an aneurysmatic interauricular septum with transoesophageal echocardiogram showing an interauricular communication ostium secundum type with a septal aneurysm that led to a right to left shut. The anatomical and clinical features did not allowed percutaneous or surgical closure. After a group discussion we decided for palliative care. Discussion: POS due to a septal aneurysm with right-to-left shunt is a rare and difficult diagnose because it requires a detailed medical examination and exclusion of any other possible diseases. Besides that, it is a very rare presentation in such an old person.

IntroductionCurrently, the most common chronic metabolic disease in our society is Diabetes Mellitus. The diagnosis of Diabetes Mellitus supposes an impact for the patient, since it requires a modification in the lifestyle, which demands a great capacity for adaptation and modification of habits. The aim of the study was to determine whether personality factors and health status influence resilience and coping strategies in a sample of healthy and diabetic subjects.MethodologyThe sample included a total of 401 subjects (201 patients with Diabetes and 200 without pathology). The instruments applied for data collection were: Sociodemographic data questionnaire, the Resilience Scale, the Coping Strategies Questionnaire and The “Big Five” factor taxonomy. The data collection period was approximately 2 years (between February 2018 and January 2020).ResultsCertain personality factors, such as Emotional Stability, Integrity, Conscientiousness and Extraversion, were positively related to Resilience. Additionally, Emotional Stability, Integrity, and Extraversion were positively associated with Rational Coping. On the other hand, emotional stability, agreeableness and extraversion were negatively related to emotional coping. In relation to health status, the absence of pathology is related to the use of rational strategies more than to the diagnosis of diabetes. Therefore, the participants in this study present different psychological patterns depending on personality and health status.ConclusionsThe present study shows that the subjects of the sample present different psychological patterns depending on Personality and health status.

En la investigación se hace un análisis de los índices termodinámicos tradicionales, en diferentes situaciones sinópticas propias de los periodos poco lluvioso y lluvioso en Cuba en los años 2008 y 2019, en las que ocurrieron Tormentas Eléctricas. El objetivo fundamental es determinar cuál o cuáles de esos índices, se ajusta mejor a diferentes configuraciones sinópticas, así como determinar un índice derivado que mejore la predicción de las tormentas eléctricas en la región occidental de Cuba. Se utilizaron datos del Global Forecast System (GFS) para alimentar el modelo mesoescalar Weather Research Forecast /Advanced Research (WRF-ARW), con una resolución espacial de 4 kilómetros, que abarca la región occidental de Cuba; la cronología de los frentes fríos y la de las tormentas eléctricas para la selección de los casos de estudios. Las correlaciones determinaron que los índices K, TT y GDI, fueron los que mostraron las mayores correlaciones siendo superiores a los 0.5. Con estos resultados se determinó el índice derivado, con el que se obtuvo una detección de los procesos convectivos en el área de estudio del 73 y 75 % para el periodo poco lluvioso y el lluvioso respectivamente.

Coronary heart disease (CHD) is one of the most pressing health problems of our time and a major cause of preventable death. CHD results from complex interactions between genetic and environmental factors. Using multiplex serological testing for persistent or frequently recurring infections and genome-wide analysis in a prospective population study, we delineate the respective and combined influences of genetic variation, infections, and low-grade inflammation on the risk of incident CHD. Study participants are enrolled in the CoLaus|PsyCoLaus study, a longitudinal, population-based cohort with baseline assessments from 2003 through 2008 and follow-up visits every 5 years. We analyzed a subgroup of 3459 individuals with available genome-wide genotyping data and immunoglobulin G levels for 22 persistent or frequently recurring pathogens. All reported CHD events were evaluated by a panel of specialists. We identified independent associations with incident CHD using univariable and multivariable stepwise Cox proportional hazards regression analyses. Of the 3459 study participants, 210 (6.07%) had at least one CHD event during the 12 years of follow-up. Multivariable stepwise Cox regression analysis, adjusted for known cardiovascular risk factors, socioeconomic status, and statin intake, revealed that high polygenic risk (hazard ratio [HR] 1.31, 95% CI 1.10–1.56, p=2.64 × 10−3) and infection with Fusobacterium nucleatum (HR 1.63, 95% CI 1.08–2.45, p=1.99 × 10−2) were independently associated with incident CHD. In a prospective, population-based cohort, high polygenic risk and infection with F. nucleatum have a small, yet independent impact on CHD risk.

Abstract The objective of this study was to evaluate plant cover and dry mass produced by green manures and their influence on the chemical attributes of soil for the ecological restoration of an area around bauxite mine environment in Southeast Brazil. Soil preparation, chemical fertilization and sowing of three species of green manures were carried out: Cajanus cajan, Crotalaria juncea and Stylosanthes guianensis. In general, C. cajan and C. juncea showed the best performance in plant cover. C. cajan and S. guianensis produced more phytomass. Green manures were efficient to promote rapid plant cover and increase in soil organic matter and total-N contents in all treatments. Therefore, green manure is a viable alternative that contributes to the success of ecological restoration projects in areas in the bauxite mining environment.

Toxocariasis is one of the most widespread and important zoonotic parasitic diseases, although neglected. Data regarding human Toxocara infection in Portugal are almost absent. This article gives an overview of the situation of toxocariasis in Portugal over the last decade based on casuistic data. A total of 846 serum samples from individuals suspected of toxocariasis, collected from 2010 to 2020, were analyzed at the Institute of Hygiene and Tropical Medicine. Sera were tested for IgG antibodies to Toxocara canis excreted–secreted larval antigens by enzyme-linked immunosorbent assay and counterimmunoelectrophoresis. Positivity was detected in 18.8% (159/846) [CI 95%: 16.3–21.6], with positives detected throughout continental Portugal. Overall, 59.7% of the positives were diagnosed in younger than 20 years (35.2% aged 0–9 years and 24.5% aged 10–19 years). Eosinophilia was the most frequent feature reported (27.7%). Pediatrics (41.5%) and Infectiology (25.8%) were the specialties with the highest number of positives. An average of 77 samples/year were received, recording a maximum positivity in 2012 (41.5%, n = 27/65) and a minimum in 2020 (6.4%, n = 3/47). These numbers may reflect the effectiveness of current preventive measures, highlighting the need to maintain public awareness to control this helminthozoonosis and promote a higher public health standard.

Additional costs for seismic safety are evaluated in shallow foundation projects for five reinforced concrete buildings with different dimensions in plan and prop, in the city of Santiago de Cuba, an area of greatest seismic danger in Cuba. The direct costs of the foundation plates for seismic, wind load and gravity loads are compared separately. The physical mechanical properties of the soil were obtained from testing soil samples obtained by SPT test at the construction site. The requirements for the design of the foundation plates result from the structural modeling using SAP 2000 NL version 14 for the load combinations recommended by current Cuban regulations. For the geotechnical and structural design of the foundation, the Excel book DISCAR 3.0 was used. The budgets were calculated using the PRESWIN program, showing increases in the costs for seismic safety of the foundation plates between 34 to 66% in relation to similar costs obtained by wind loads or gravitational loads, significantly increasing the dimensions of the foundation’s plates, concrete and excavation volumes as well as total reinforcing steel weight for seismic loads.

La mejora del proceso de enseñanza-aprendizaje debe partir del diagnóstico de su estado real. Esta investigación descriptiva tiene como objetivo caracterizar el estado actual del proceso de enseñanza-aprendizaje del contenido magnitudes en el primer ciclo del nivel primario. En la investigación se utilizaron métodos y técnicas como el análisis de documentos, la observación a clases, la entrevista y la prueba pedagógica. En el análisis de datos cualitativos se utilizó el software profesional AQUAD 7. Los resultados alcanzados evidencian las carencias de la actual concepción del proceso de enseñanza-aprendizaje del contenido magnitudes, que no llega a revelar los procederes metodológicos para propiciar la asimilación activa de los conceptos de objetos, relaciones y procedimientos del trabajo con este contenido.

The demands of high performance sport and the changes that have been taking place in it, demand adequate preparation of future female volleyball players of school categories. In a documentary search it was possible to verify the non-existence of parameters and real values at national level on the physical, technical and tactical behavior of volleyball teams of school categories in Cuba. Based on this, the objective of the present work is focused on analyzing the competitive performance of 13-15-year-old female volleyball players in Havana, where certain indicators were used to evaluate the behavior of the players during the competition, among them: percentage of points obtained by own actions or errors of the opponent, as well as the number of jumps per game, set and match functions. Scientific methods were used such as direct observation to evaluate the desired elements; surveys to coaches; review of documents and mathematical methods to tabulate the information that generates important demands for the adjustment in the training loads that will be applied to the athletes in the next season. The control of these parameters is of importance in the planning of the preparation of the athletes, allowing the coaches to develop in an effective way the process of training management in these categories.

Motivados pelo processo histórico e social presente na formação de mais de cento e cinquenta comunidades remanescentes de quilombos no estado do Amapá, e, como parte de um estudo macro de doutoramento que tem como temática: “A apropriação das práticas de numeramento na EJA/PROEJA em comunidades quilombolas no Estado do Amapá – Brasil.” é que este trabalho se alicerça, objetivando compreender uma análise do perfil dos docentes e dos espaços escolares quilombolas localizados geograficamente no estado Amapá, uma das vinte e sete unidades federativas do Brasil, no extremo norte do país, e que compõem a Amazônia Ocidental. A metodologia adotada foi a pesquisa de campo, realizada através da aplicação de questionários e da observação direta da realidade social em que os sujeitos presentes na pesquisa estão inseridos. Os resultados aqui apresentados, evidenciam a percepção dos professores e dos membros das comunidades entrevistadas, no que tange os aspectos dos mitos, tradições e da cosmogonia. Tais percepções apontam para a necessidade de um estudo que valorize os aspectos históricos, sociais e culturais das comunidades quilombolas e que os currículos levem em consideração o disposto previsto na Lei 10.639/2003 (BRASIL, 2003). Espera-se que os dados aqui apresentados contribuam com novas produções que discorram sobre a realidade das comunidades quilombolas, que gere reflexões sobre o conteúdo presente no currículo escolar e que fortaleça as pesquisas em etnociências no país.

Este estudo teve como objetivo testar a eficiência do uso de um novo equipamento denominado nucleário, na sobrevivência e crescimento de mudas de espécies arbóreas visando à restauração florestal em áreas atingidas pelo rejeito da barragem de Fundão, em Mariana, MG. Foram avaliadas, por 12 meses, duas espécies arbóreas nativas (Piptadenia gonoacantha (Mart.) J.F. Macbr. e Inga edulis Mart.), sob um desenho de blocos aleatorizados e três tratamentos: hidrogel (T1), nucleário (T2) e controle (T3). Analisou-se o efeito dos tratamentos e do tempo após o plantio sobre a sobrevivência e crescimento das mudas, por comparação múltipla de médias e modelos lineares mistos. Foram observadas diferenças significativas de crescimento em altura e em diâmetro ao nível do solo (DNS) entre os tratamentos. As taxas de crescimento relativo para altura e DNS das duas espécies se mantiveram relativamente constantes, sem diferenças entre os tratamentos. Constatou-se que I. edulis apresentou sobrevivência maior na presença do nucleário, em comparação com os demais tratamentos. Os resultados indicam que apenas o uso de nucleários não foi suficiente para garantir o bom desenvolvimento e sobrevivência das mudas em campo, sendo recomendada sua avaliação para outras espécies arbóreas nativas.

Type II DNA topoisomerases relax topological stress by transiently gating DNA passage in a controlled cut-and-reseal mechanism that affects both DNA strands. Therefore, they are essential to overcome topological problems associated with DNA metabolism. Their aberrant activity results in the generation of DNA double-strand breaks, which can seriously compromise cell survival and genome integrity. Here, we profile the transcriptome of human-telomerase-immortalized retinal pigment epithelial 1 (RPE-1) cells when treated with merbarone, a drug that catalytically inhibits type II DNA topoisomerases. We performed RNA-Seq after 4 and 8 h of merbarone treatment and compared transcriptional profiles versus untreated samples. We report raw sequencing data together with lists of gene counts and differentially expressed genes.

In this paper, we determine the magnetic moment induced in graphene when grown on a cobalt film using polarised neutron reflectivity (PNR). A magnetic signal in the graphene was detected by X-ray magnetic circular dichroism (XMCD) spectra at the C K-edge. From the XMCD sum rules an estimated magnetic moment of 0.3 μB/C atom, while a more accurate estimation of 0.49 μB/C atom was obtained by carrying out a PNR measurement at 300 K. The results indicate that the higher magnetic moment in Co is counterbalanced by the larger lattice mismatch between the Co-C (1.6%) and the slightly longer bond length, inducing a magnetic moment in graphene that is similar to that reported in Ni/graphene heterostructures.