312 results on '"Pedreira, C"'
Search Results
2. Smoking-attributable mortality by sex in the 27 Brazilian federal units: 2019
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Wanderlei-Flores, B., Rey-Brandariz, J., Rodrigues Pinto Corrêa, P.C., Ruano-Ravina, A., Guerra-Tort, C., Candal-Pedreira, C., Varela-Lema, L., Montes, A., and Pérez-Ríos, M.
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- 2024
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3. Effectiveness of smoking cessation interventions among pregnant women: An updated systematic review and meta-analysis
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Vila-Farinas, A., Pérez-Rios, M., Montes-Martinez, A., Ruano-Ravina, A., Forray, Ariadna, Rey-Brandariz, J., Candal-Pedreira, C., Fernández, E., Casal-Acción, B., and Varela-Lema, L.
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- 2024
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4. Feature space partition: a local–global approach for classification
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Marcelino, C. G. and Pedreira, C. E.
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- 2022
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5. Smoking-attributable mortality in Portugal and its regions in 2019
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Rey-Brandariz, J., Ravara, S., López-Vizcaíno, E., Santiago-Pérez, M.I., Ruano-Ravina, A., Candal-Pedreira, C., Varela-Lema, L., Mourino, N., Aguiar, P., and Pérez-Ríos, M.
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- 2023
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6. Hospitalizations due to and with chronic obstructive pulmonary disease in Galicia: 20 years of evolution
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Fernández-García, A., Pérez-Ríos, M., Fernández-Villar, A., Candal-Pedreira, C., Naveira-Barbeito, G., Santiago-Pérez, M.I., Rey-Brandariz, J., Represas-Represas, C., Malvar-Pintos, A., and Ruano-Ravina, A.
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- 2022
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7. Hospitalizaciones por y con enfermedad pulmonar obstructiva crónica en Galicia: 20 años de evolución
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Fernández-García, A., Pérez-Ríos, M., Fernández-Villar, A., Candal-Pedreira, C., Naveira-Barbeito, G., Santiago-Pérez, M.I., Rey-Brandariz, J., Represas-Represas, C., Malvar-Pintos, A., and Ruano-Ravina, A.
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- 2022
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8. Where Do Chronic Obstructive Pulmonary Disease Patients Die? 8-Year Trend, with Special Focus on Sex-Related Differences
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Fernández-García A, Pérez-Ríos M, Candal-Pedreira C, Represas-Represas C, Fernández-Villar A, Santiago-Pérez MI, Rey-Brandariz J, Naveira-Barbeito G, Malvar-Pintos A, and Ruano-Ravina A
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pulmonary disease ,chronic obstructive ,place ,death ,hospital ,Diseases of the respiratory system ,RC705-779 - Abstract
Alberto Fernández-García,1 Mónica Pérez-Ríos,1– 3 Cristina Candal-Pedreira,1,3 Cristina Represas-Represas,4,5 Alberto Fernández-Villar,4,5 María Isolina Santiago-Pérez,1,6 Julia Rey-Brandariz,1 Gael Naveira-Barbeito,6 Alberto Malvar-Pintos,6 Alberto Ruano-Ravina1– 3 1Department of Preventive Medicine and Public Health, University of Santiago de Compostela, Santiago de Compostela, Spain; 2Consortium for Biomedical Research in Epidemiology and Public Health (Ciber en Epidemiología y Salud Pública/CIBERESP), Madrid, Spain; 3Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, Spain; 4Respiratory Medicine, Alvaro Cunqueiro University Teaching Hospital, Vigo, Spain; 5Grupo NeumoVigo I+i, Instituto de Investigación Sanitaria Galicia Sur (IISGS), Vigo, Spain; 6Epidemiology Unit, Galician Health Authority, Xunta de Galicia, Santiago de Compostela, SpainCorrespondence: Mónica Pérez-Ríos, Department of Preventive Medicine and Public Health, University of Santiago de Compostela, C/ San Francisco s/n, Santiago de Compostela, 15782, Spain, Tel +34-981-581237, Fax +34-981-572282, Email monica.perez.rios@usc.esBackground: To plan end-of-life care it is essential to ascertain where patients die. There is very little information on the place of death of chronic obstructive pulmonary disease (COPD) patients. Accordingly, this study set out to describe the place of death of all COPD-related deaths in a Spanish region across the period 2009– 2017, taking into account the sex and age of the deceased.Methods: We analyzed COPD deaths, codes J41-44 of the International Classification of Diseases-10th revision, in the Galician Autonomous Region from 2009 to 2017. Using death certificate data furnished by the Galician Mortality Registry, information was extracted on place of death, categorized as “hospital”, “nursing home”, “patient’s home”, “other” or “not shown”.Results: There were 10,274 deaths, with a male:female ratio of 2.52; 39.0% of deaths occurred in hospital and 41.4% at home, with these data varying according to sex and age. Across the study period, no reduction was observed in the number of deaths that occurred in hospital. For all the period analyzed, deaths among women occurred mostly at home, with an increase being seen in the number of deaths in nursing homes over the course of the study. Patients aged under 70 years tended to die more frequently in hospital, and those over this age died more frequently at home or in nursing homes.Conclusion: A very high percentage of COPD patients still die in hospital, a trend that has shown no decline in recent years. Even so, there are important variations by sex and age on the place of death of these patients.Keywords: pulmonary disease, chronic obstructive, death, hospital
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- 2022
9. Factors Related with Hospital Attendance and Mortality in Patients with COPD: A Case–Control Study in a Real-Life Setting
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López-Pardo ME, Candal-Pedreira C, Valdés-Cuadrado L, Represas-Represas C, Ruano-Ravina A, and Pérez-Ríos M
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case-control study ,copd ,hospital discharge ,mortality ,Diseases of the respiratory system ,RC705-779 - Abstract
María Estrella López-Pardo,1 Cristina Candal-Pedreira,2 Luis Valdés-Cuadrado,3 Cristina Represas-Represas,4 Alberto Ruano-Ravina,2,5,6 Mónica Pérez-Ríos2,5,6 1Department of Planning and Health Reform, Galician Health Service, Santiago de Compostela, Spain; 2Department of Preventive Medicine and Public Health, School of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain; 3Department of Pulmonology, University Clinical Teaching Hospital of Santiago de Compostela, Santiago de Compostela, Spain; 4Respiratory Medicine, Alvaro Cunqueiro University Teaching Hospital, Vigo, Spain; 5Consortium for Biomedical Research in Epidemiology and Public Health (CIBER en Epidemiología y Salud Pública/CIBERESP), Madrid, Spain; 6Health Research Institute of Santiago de Compostela (Instituto de Investigación Sanitaria de Santiago de Compostela - IDIS), Santiago de Compostela, SpainCorrespondence: Alberto Ruano-Ravina, Departamento de Medicina Preventiva y Salud Pública, Facultad de Medicina, University of Santiago de Compostela, C/ San Francisco s/n, Santiago de Compostela, 15782, Spain, Tel +34-981-581237, Fax +34-981-572282, Email alberto.ruano@usc.esIntroduction: The rising trend in hospital admissions among patients with chronic obstructive pulmonary disease (COPD) is worrying, not only because of the increasing costs, but also because of the worsening quality of life. We aimed to identify the predictive factors of hospital admission, re-admission and mortality of COPD patients through using information exclusively registered in electronic clinical records.Methods: We conducted a population-based case–control study. All data were sourced from the different information systems comprising the Galician Health Service electronic record database. We included in the study patients diagnosed with COPD (code R95 in the medical record), ≥ 35 years old and with at least one spirometry performed ≤ 3 years prior inclusion. We fitted three logistic regression models, each one to ascertain the factors that influence the probability of admission, re-admission, and mortality, and calculated odds ratios (OR) with their 95% confidence intervals (95% CI).Results: COPD patients were admitted due to respiratory causes a mean of 1.51 times across the period December 2016–December 2017, with 55% requiring re-admission in the next 90 days. The factor most closely associated with the re-admission profile was home oxygen therapy (OR 3.06 95% CI 2.42– 3.87), followed by male gender (OR 2.01 95% CI 1.48– 2.72), a CHA2D-VASc scale score > 2 (OR 1.28 95% CI 1.16– 1.42), and severity by clinical risk group stratification (OR 1.14 95% CI 1.04– 1.26). Male sex (OR 1.47 CI 95% 1.04– 2.09), having been readmitted ≥ 2 times (OR 1.34 CI 95% 1.11– 1.61) and being ≥ 70 years old (OR 1.05 CI 95% 1.03– 1.08) increase the probability of dying from COPD during the study period.Conclusion: These results confirm the complexity of management of COPD exacerbations, and indicate the need to establish strategies that would ensure continuity of care after hospital admission, with the aim of preventing re-admissions and death.Keywords: case–control study, COPD, hospital discharge, mortality
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- 2022
10. Lung cancer risk associated with occupations in women: a pooling study.
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Torres-Cadavid, E, Pérez-Ríos, M, Candal-Pedreira, C, Guerra-Tort, C, Rey-Brandariz, J, Provencio-Pulla, M, Kelsey, K, and Ruano-Ravina, A
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LUNG cancer ,WHITE collar workers ,CANCER patients ,WOMEN'S employment ,WOMEN employees - Abstract
Background Occupation is an important risk factor for lung cancer. This knowledge is mainly based on studies conducted on men, with the results being generalized to women. Aims We aimed to identify the relationship between different occupations and lung cancer in women. Methods Pooling study in which data were pooled from six case–control studies conducted at 13 Spanish hospitals and 1 hospital in Portugal. Each woman's longest held job was coded as per the ISCO-08. Results were adjusted for age, smoking, and exposure to residential radon. Results The study population comprised 1262 women: 618 cases and 644 controls. The reference group were white-collar workers. The adjusted multivariate analysis showed a higher risk of developing lung cancer among teaching professionals (odds ratio [OR]: 4.36; 95% confidence interval [CI] 1.73–11.02), cooks (OR: 3.59; 95% CI 1.52–8.48), domestic cleaners and helpers (OR: 2.98; 95% CI 1.54–5.78), homemakers (OR: 2.30; 95% CI 1.26–4.21) and crop farmers, livestock farmers and gardeners (OR: 2.06, 95% CI: 1.11–3.81). For adenocarcinoma, the highest risk was observed in teaching professionals, and for small-cell carcinoma, the highest risk was observed in cooks. Higher risks were observed for small-cell carcinoma compared to other histological types. Conclusions Some occupations may be associated with an increased risk of lung cancer in women and this risk could vary by histologic subtype; however, further research is needed to confirm these associations. In any case, protection measures must be implemented in the workplace aimed at reducing the risk of lung cancer among women workers, and more studies exclusively focused on women are warranted. [ABSTRACT FROM AUTHOR]
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- 2024
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11. EP01.01-01 Adenocarcinoma and Small Cell Lung Cancer Risk Associated with Occupation in Women
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Ruano Ravina, A., primary, Candal-Pedreira, C., additional, Torres, E., additional, Rey-Brandariz, J., additional, Guerra-Tort, C., additional, Varela-Lema, L., additional, and Pérez-Ríos, M., additional
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- 2023
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12. EP01.01-21 Respirable Crystalline Silica Exposure and Lung Cancer Risk: A Review of Cut-off Points
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Rey Brandariz, J., primary, Pérez Ríos, M., additional, Candal Pedreira, C., additional, Martínez, C., additional, Varela-Lema, L., additional, and Ruano-Ravina, A., additional
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- 2023
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13. P1.01-04 Clinical and Genetic Characteristics in Younger vs Older Lung Cancer Patients
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Candal-Pedreira, C., primary, Ruano-Ravina, A., additional, Calvo de Juan, V., additional, Cobo, M., additional, Trigo, J.M., additional, Carcereny, E., additional, Cucurull, M., additional, López Castro, R., additional, Solís García, E., additional, Sánchez-Gastaldo, A., additional, Massutí, B., additional, Rodríguez-Abreu, D., additional, Estival, A., additional, Guirado Risueño, M., additional, Rey-Brandariz, J., additional, Pamiés Ramón, M., additional, García Campelo, R., additional, Alonso-Jáudenes, G., additional, Camps, C., additional, del Barco Morillo, E., additional, González Ojea, C., additional, Dómine, M., additional, Sánchez-Hernández, A., additional, Bosch-Barrera, J., additional, Sala González, M.Á., additional, and Provencio, M., additional
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- 2023
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14. EP01.01-25 Occupational Exposure and Lung Cancer Risk in Women: A Pooling Study
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Candal-Pedreira, C., primary, Torres, E., additional, Rey-Brandariz, J., additional, Guerra-Tort, C., additional, Varela-Lema, L., additional, Pérez-Ríos, M., additional, and Ruano-Ravina, A., additional
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- 2023
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15. Effectiveness of Smoking Cessation Interventions among Pregnant Women: an Updated Systematic Review and Meta-Analysis
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Vila-Farinas, A., primary, Pérez-Rios, M., additional, Montes-Martinez, A., additional, Ruano-Ravina, A., additional, Forray, Ariadna, additional, Rey-Brandariz, J., additional, Candal-Pedreira, C., additional, Fernández, E., additional, Casal-Acción, B., additional, and Varela-Lema, L., additional
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- 2023
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16. Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution
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European Commission, Junta de Castilla y León, Instituto de Salud Carlos III, Consejo Superior de Investigaciones Científicas (España), Universidad de Salamanca, Instituto de Investigación Biomédica de Salamanca, Landeira-Viñuela, Alicia, Alcoceba, Miguel, Navarro-Bailón, Almudena, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Sánchez-Santos, Jose Manuel, Lécrevisse, Quentin, Pedreira, C. E., García-Vaquero, Marina L., Hernández, Ángela-Patricia, Montalvillo, Enrique, Góngora, Rafael, Rivas, Javier de las, González-Díaz, Marcos, Orfao, Alberto, Fuentes, Manuel, European Commission, Junta de Castilla y León, Instituto de Salud Carlos III, Consejo Superior de Investigaciones Científicas (España), Universidad de Salamanca, Instituto de Investigación Biomédica de Salamanca, Landeira-Viñuela, Alicia, Alcoceba, Miguel, Navarro-Bailón, Almudena, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Sánchez-Santos, Jose Manuel, Lécrevisse, Quentin, Pedreira, C. E., García-Vaquero, Marina L., Hernández, Ángela-Patricia, Montalvillo, Enrique, Góngora, Rafael, Rivas, Javier de las, González-Díaz, Marcos, Orfao, Alberto, and Fuentes, Manuel
- Abstract
Chronic lymphocytic leukemia (CLL) is the most common leukemia in the Western world. Studies of CLL antibody reactivity have shown differential targets to autoantigens and antimicrobial molecular motifs that support the current hypothesis of CLL pathogenesis. Methods: In this study, we conducted a quantitative serum analysis of 7 immunoglobulins in CLL and monoclonal B-cell lymphocytosis (MBL) patients (bead-suspension protein arrays) and a serological profile (IgG and IgM) study of autoantibodies and antimicrobial antigens (protein microarrays). Results: Significant differences in the IgA levels were observed according to disease progression and evolution as well as significant alterations in IgG1 according to IGHV mutational status. More representative IgG autoantibodies in the cohort were against nonmutagenic proteins and IgM autoantibodies were against vesicle proteins. Antimicrobial IgG and IgM were detected against microbes associated with respiratory tract infections. Conclusions: Quantitative differences in immunoglobulin serum levels could be potential biomarkers for disease progression. In the top 5 tumoral antigens, we detected autoantibodies (IgM and IgG) against proteins related to cell homeostasis and metabolism in the studied cohort. The top 5 microbial antigens were associated with respiratory and gastrointestinal infections; moreover, the subsets with better prognostics were characterized by a reactivation of Cytomegalovirus. The viral humoral response could be a potential prognosis biomarker for disease progression.
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- 2023
17. Automated identification of leukocyte subsets improves standardization of database-guided expert-supervised diagnostic orientation in acute leukemia: a EuroFlow study
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Lhermitte, L, Barreau, S, Morf, D, Fernandez, P, Grigore, G, Barrena, S, de Bie, M, Flores-Montero, J, Bruggemann, M, Mejstrikova, E, Nierkens, S, Burgos, L, Caetano, J, Gaipa, G, Buracchi, C, da Costa, E, Sedek, L, Szczepanski, T, Aanei, C, van der Sluijs-Gelling, A, Delgado, A, Fluxa, R, Lecrevisse, Q, Pedreira, C, van Dongen, J, Orfao, A, van der Velden, V, Bitter, W, Lubbers, B, Teodosio, C, Zlei, M, de Bie, F, de Bruin-Versteeg, S, van der Burg, M, Schilham, M, Langerak, A, te Marvelde, J, Bras, A, Schilperoord-Vermeulen, J, Jugooa, R, Heezen, K, Almeida, J, Vidriales, M, Perez-Andres, M, Matarraz, S, Martin, L, Perez-Moran, J, Puig, N, Almeida, A, Gomes da Silva, M, Faria, T, Ritgen, M, Szczepanowski, M, Kohlscheen, S, Laqua, A, Harbst, E, Finke, J, Asnafi, V, Duroyon, E, Trka, J, Hrusak, O, Kalina, T, Novakova, M, Thurner, D, Kanderova, V, Bulsa, J, Slota, L, Kulis, J, de Jong, A, de Koning, A, Lima, M, Santos, A, Bottcher, S, Lange, S, Engelmann, R, Paape, D, Machka, C, Burracchi, C, Bugarin, C, Lopez-Granados, E, del Pino Molina, L, Campos-Guyotat, L, Miguel, J, Paiva, B, Villamor-Casas, N, Magnano, L, Philippe, J, Bonroy, C, Denys, B, Willems, A, Breughe, P, de Wolf, J, Sousa, A, Silva, S, Lhermitte L., Barreau S., Morf D., Fernandez P., Grigore G., Barrena S., de Bie M., Flores-Montero J., Bruggemann M., Mejstrikova E., Nierkens S., Burgos L., Caetano J., Gaipa G., Buracchi C., da Costa E. S., Sedek L., Szczepanski T., Aanei C. -M., van der Sluijs-Gelling A., Delgado A. H., Fluxa R., Lecrevisse Q., Pedreira C. E., van Dongen J. J. M., Orfao A., van der Velden V. H. J., Bitter W. M., Lubbers B. R., Teodosio C. I., Zlei M., van der Sluijs-Gelling A. J., de Bie F., de Bruin-Versteeg S., van der Burg M., Schilham M. W., Langerak A. W., te Marvelde J., Bras A. E., Schilperoord-Vermeulen J., Jugooa R., Heezen K. C., Almeida J., Vidriales M. B., Perez-Andres M., Matarraz S., Martin L., Perez-Moran J. J., Puig N., Almeida A. M., Gomes da Silva M., Faria T., Ritgen M., Szczepanowski M., Kohlscheen S., Laqua A., Harbst E., Finke J., Asnafi V., Duroyon E., Trka J., Hrusak O., Kalina T., Novakova M., Thurner D., Kanderova V., Bulsa J., Slota L., Kulis J., de Jong A., de Koning A., Lima M., Santos A. H., Bottcher S., Lange S., Engelmann R., Paape D., Machka C., Burracchi C., Bugarin C., Lopez-Granados E., del Pino Molina L., Campos-Guyotat L., Aanei C., Miguel J. F. S., Paiva B., Villamor-Casas N., Magnano L., Philippe J., Bonroy C., Denys B., Willems A., Breughe P., de Wolf J., Sousa A. E., Silva S. L., Lhermitte, L, Barreau, S, Morf, D, Fernandez, P, Grigore, G, Barrena, S, de Bie, M, Flores-Montero, J, Bruggemann, M, Mejstrikova, E, Nierkens, S, Burgos, L, Caetano, J, Gaipa, G, Buracchi, C, da Costa, E, Sedek, L, Szczepanski, T, Aanei, C, van der Sluijs-Gelling, A, Delgado, A, Fluxa, R, Lecrevisse, Q, Pedreira, C, van Dongen, J, Orfao, A, van der Velden, V, Bitter, W, Lubbers, B, Teodosio, C, Zlei, M, de Bie, F, de Bruin-Versteeg, S, van der Burg, M, Schilham, M, Langerak, A, te Marvelde, J, Bras, A, Schilperoord-Vermeulen, J, Jugooa, R, Heezen, K, Almeida, J, Vidriales, M, Perez-Andres, M, Matarraz, S, Martin, L, Perez-Moran, J, Puig, N, Almeida, A, Gomes da Silva, M, Faria, T, Ritgen, M, Szczepanowski, M, Kohlscheen, S, Laqua, A, Harbst, E, Finke, J, Asnafi, V, Duroyon, E, Trka, J, Hrusak, O, Kalina, T, Novakova, M, Thurner, D, Kanderova, V, Bulsa, J, Slota, L, Kulis, J, de Jong, A, de Koning, A, Lima, M, Santos, A, Bottcher, S, Lange, S, Engelmann, R, Paape, D, Machka, C, Burracchi, C, Bugarin, C, Lopez-Granados, E, del Pino Molina, L, Campos-Guyotat, L, Miguel, J, Paiva, B, Villamor-Casas, N, Magnano, L, Philippe, J, Bonroy, C, Denys, B, Willems, A, Breughe, P, de Wolf, J, Sousa, A, Silva, S, Lhermitte L., Barreau S., Morf D., Fernandez P., Grigore G., Barrena S., de Bie M., Flores-Montero J., Bruggemann M., Mejstrikova E., Nierkens S., Burgos L., Caetano J., Gaipa G., Buracchi C., da Costa E. S., Sedek L., Szczepanski T., Aanei C. -M., van der Sluijs-Gelling A., Delgado A. H., Fluxa R., Lecrevisse Q., Pedreira C. E., van Dongen J. J. M., Orfao A., van der Velden V. H. J., Bitter W. M., Lubbers B. R., Teodosio C. I., Zlei M., van der Sluijs-Gelling A. J., de Bie F., de Bruin-Versteeg S., van der Burg M., Schilham M. W., Langerak A. W., te Marvelde J., Bras A. E., Schilperoord-Vermeulen J., Jugooa R., Heezen K. C., Almeida J., Vidriales M. B., Perez-Andres M., Matarraz S., Martin L., Perez-Moran J. J., Puig N., Almeida A. M., Gomes da Silva M., Faria T., Ritgen M., Szczepanowski M., Kohlscheen S., Laqua A., Harbst E., Finke J., Asnafi V., Duroyon E., Trka J., Hrusak O., Kalina T., Novakova M., Thurner D., Kanderova V., Bulsa J., Slota L., Kulis J., de Jong A., de Koning A., Lima M., Santos A. H., Bottcher S., Lange S., Engelmann R., Paape D., Machka C., Burracchi C., Bugarin C., Lopez-Granados E., del Pino Molina L., Campos-Guyotat L., Aanei C., Miguel J. F. S., Paiva B., Villamor-Casas N., Magnano L., Philippe J., Bonroy C., Denys B., Willems A., Breughe P., de Wolf J., Sousa A. E., and Silva S. L.
- Abstract
Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant classification panel and final diagnosis. In this study, we designed and validated an algorithm for automated (database-supported) gating and identification (AGI tool) of cell subsets within samples stained with ALOT. A reference database of normal peripheral blood (PB, n = 41) and bone marrow (BM; n = 45) samples analyzed with the ALOT was constructed, and served as a reference for the AGI tool to automatically identify normal cells. Populations not unequivocally identified as normal cells were labeled as checks and were classified by an expert. Additional normal BM (n = 25) and PB (n = 43) and leukemic samples (n = 109), analyzed in parallel by experts and the AGI tool, were used to evaluate the AGI tool. Analysis of normal PB and BM samples showed low percentages of checks (<3% in PB, <10% in BM), with variations between different laboratories. Manual analysis and AGI analysis of normal and leukemic samples showed high levels of correlation between cell numbers (r2 > 0.95 for all cell types in PB and r2 > 0.75 in BM) and resulted in highly concordant classification of leukemic cells by our previously published automated database-guided expert-supervised orientation tool for immunophenotypic diagnosis and classification of acute leukemia (Compass tool). Similar data were obtained using alternative, commercially available tubes, confirming the robustness of the developed tools. The AGI tool represents an innovative step in minimizing human intervention and requirements in expertise, toward a “sample-in and result-out” approach which may result in more objective and reproducible data analysis and diagnostics. The AGI tool may improve quality of immunophenotyping in individual laboratories, since high percentages of checks in normal samples are an alert on the quality of the internal p
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- 2021
18. Table_4_Unravelling soluble immune checkpoints in chronic lymphocytic leukemia: Physiological immunomodulators or immune dysfunction.xlsx [Dataset]
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Landeira-Viñuela, Alicia, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Alcoceba, Miguel, Navarro-Bailón, Almudena, Pedreira, C. E., Lécrevisse, Quentin, Díaz-Muñoz, Laura, Sanchez-Santos, Jose Manuel, Hernández, Ángela-Patricia, García-Vaquero, Marina L., Góngora, Rafael, De Las Rivas, Javier, González, Marcos, Orfao, Alberto, Fuentes, Manuel, Landeira-Viñuela, Alicia, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Alcoceba, Miguel, Navarro-Bailón, Almudena, Pedreira, C. E., Lécrevisse, Quentin, Díaz-Muñoz, Laura, Sanchez-Santos, Jose Manuel, Hernández, Ángela-Patricia, García-Vaquero, Marina L., Góngora, Rafael, De Las Rivas, Javier, González, Marcos, Orfao, Alberto, and Fuentes, Manuel
- Abstract
Chronic lymphocytic leukemia (CLL) is a lymphoid neoplasm characterized by the accumulation of mature B cells. The diagnosis is established by the detection of monoclonal B lymphocytes in peripheral blood, even in early stages [monoclonal B-cell lymphocytosis (MBLhi)], and its clinical course is highly heterogeneous. In fact, there are well-characterized multiple prognostic factors that are also related to the observed genetic heterogenicity, such as immunoglobulin heavy chain variable region (IGHV) mutational status, del17p, and TP53 mutations, among others. Moreover, a dysregulation of the immune system (innate and adaptive immunity) has been observed in CLL patients, with strong impact on immune surveillance and consequently on the onset, evolution, and therapy response. In addition, the tumor microenvironment is highly complex and heterogeneous (i.e., matrix, fibroblast, endothelial cells, and immune cells), playing a critical role in the evolution of CLL. In this study, a quantitative profile of 103 proteins (cytokines, chemokines, growth/regulatory factors, immune checkpoints, and soluble receptors) in 67 serum samples (57 CLL and 10 MBLhi) has been systematically evaluated. Also, differential profiles of soluble immune factors that discriminate between MBLhi and CLL (sCD47, sCD27, sTIMD-4, sIL-2R, and sULBP-1), disease progression (sCD48, sCD27, sArginase-1, sLAG-3, IL-4, and sIL-2R), or among profiles correlated with other prognostic factors, such as IGHV mutational status (CXCL11/I-TAC, CXCL10/IP-10, sHEVM, and sLAG-3), were deciphered. These results pave the way to explore the role of soluble immune checkpoints as a promising source of biomarkers in CLL, to provide novel insights into the immune suppression process and/or dysfunction, mostly on T cells, in combination with cellular balance disruption and microenvironment polarization leading to tumor escape.
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- 2022
19. DataSheet_1_Unravelling soluble immune checkpoints in chronic lymphocytic leukemia: Physiological immunomodulators or immune dysfunction.pdf
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Landeira-Viñuela, Alicia, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Alcoceba, Miguel, Navarro-Bailón, Almudena, Pedreira, C. E., Lécrevisse, Quentin, Díaz-Muñoz, Laura, Sanchez-Santos, Jose Manuel, Hernández, Ángela-Patricia, García-Vaquero, Marina L., Góngora, Rafael, De Las Rivas, Javier, González, Marcos, Orfao, Alberto, Fuentes, Manuel, Landeira-Viñuela, Alicia, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Alcoceba, Miguel, Navarro-Bailón, Almudena, Pedreira, C. E., Lécrevisse, Quentin, Díaz-Muñoz, Laura, Sanchez-Santos, Jose Manuel, Hernández, Ángela-Patricia, García-Vaquero, Marina L., Góngora, Rafael, De Las Rivas, Javier, González, Marcos, Orfao, Alberto, and Fuentes, Manuel
- Abstract
Chronic lymphocytic leukemia (CLL) is a lymphoid neoplasm characterized by the accumulation of mature B cells. The diagnosis is established by the detection of monoclonal B lymphocytes in peripheral blood, even in early stages [monoclonal B-cell lymphocytosis (MBLhi)], and its clinical course is highly heterogeneous. In fact, there are well-characterized multiple prognostic factors that are also related to the observed genetic heterogenicity, such as immunoglobulin heavy chain variable region (IGHV) mutational status, del17p, and TP53 mutations, among others. Moreover, a dysregulation of the immune system (innate and adaptive immunity) has been observed in CLL patients, with strong impact on immune surveillance and consequently on the onset, evolution, and therapy response. In addition, the tumor microenvironment is highly complex and heterogeneous (i.e., matrix, fibroblast, endothelial cells, and immune cells), playing a critical role in the evolution of CLL. In this study, a quantitative profile of 103 proteins (cytokines, chemokines, growth/regulatory factors, immune checkpoints, and soluble receptors) in 67 serum samples (57 CLL and 10 MBLhi) has been systematically evaluated. Also, differential profiles of soluble immune factors that discriminate between MBLhi and CLL (sCD47, sCD27, sTIMD-4, sIL-2R, and sULBP-1), disease progression (sCD48, sCD27, sArginase-1, sLAG-3, IL-4, and sIL-2R), or among profiles correlated with other prognostic factors, such as IGHV mutational status (CXCL11/I-TAC, CXCL10/IP-10, sHEVM, and sLAG-3), were deciphered. These results pave the way to explore the role of soluble immune checkpoints as a promising source of biomarkers in CLL, to provide novel insights into the immune suppression process and/or dysfunction, mostly on T cells, in combination with cellular balance disruption and microenvironment polarization leading to tumor escape.
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- 2022
20. Supplementary files of the article 'Expert-independent classification of mature B-cell neoplasms using standardized flow cytometry: a multicentric study' [Dataset]
- Author
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Böttcher, Sebastian, Engelmann, Robby, Grigore, Georgiana Emilia, Fernández, Paula, Caetano, J., Flores-Montero, Juan, Velden, Vincent H. J. van der, Novákova, Michaela, Philippé, J., Ritgen, Matthias, Burgos, Leire, Lécrevisse, Quentin, Lange, Sandra, Kalina, Tomas, Verde, Javier, Fluxá, Rafael, Dongen, J. J. M. van, Pedreira, C. E., Orfao, Alberto, Böttcher, Sebastian, Engelmann, Robby, Grigore, Georgiana Emilia, Fernández, Paula, Caetano, J., Flores-Montero, Juan, Velden, Vincent H. J. van der, Novákova, Michaela, Philippé, J., Ritgen, Matthias, Burgos, Leire, Lécrevisse, Quentin, Lange, Sandra, Kalina, Tomas, Verde, Javier, Fluxá, Rafael, Dongen, J. J. M. van, Pedreira, C. E., and Orfao, Alberto
- Published
- 2022
21. Supplementary files of the article 'Deciphering biomarkers for leptomeningeal metastasis in malignant hemopathies (Lymphoma/Leukemia) patients by comprehensive multipronged proteomics characterization of cerebrospinal fluid' [Dataset]
- Author
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Juanes-Velasco, Pablo, Galicia, N., Pin, Elisa, Jara-Acevedo, Ricardo, Carabias-Sánchez, Javier, García-Valiente, R., Lécrevisse, Quentin, Pedreira, C. E., Góngora, Rafael, Sanchez-Santos, Jose Manuel, Lorenzo-Gil, Héctor, Landeira-Viñuela, Alicia, Bareke, Halin, Orfao, Alberto, Nilsson, Peter, Fuentes, Manuel, Juanes-Velasco, Pablo, Galicia, N., Pin, Elisa, Jara-Acevedo, Ricardo, Carabias-Sánchez, Javier, García-Valiente, R., Lécrevisse, Quentin, Pedreira, C. E., Góngora, Rafael, Sanchez-Santos, Jose Manuel, Lorenzo-Gil, Héctor, Landeira-Viñuela, Alicia, Bareke, Halin, Orfao, Alberto, Nilsson, Peter, and Fuentes, Manuel
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- 2022
22. Unravelling soluble immune checkpoints in chronic lymphocytic leukemia: Physiological immunomodulators or immune dysfunction
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European Commission, Junta de Castilla y León, Fundación Memoria de D. Samuel Solorzano Barruso, Instituto de Salud Carlos III, Universidad de Salamanca, Consejo Superior de Investigaciones Científicas (España), Instituto de Investigación Biomédica de Salamanca, Landeira-Viñuela, Alicia, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Alcoceba, Miguel, Navarro-Bailón, Almudena, Pedreira, C. E., Lécrevisse, Quentin, Díaz-Muñoz, Laura, Sanchez-Santos, Jose Manuel, Hernández, Ángela-Patricia, García-Vaquero, Marina L., Góngora, Rafael, De Las Rivas, Javier, González, Marcos, Orfao, Alberto, Fuentes, Manuel, European Commission, Junta de Castilla y León, Fundación Memoria de D. Samuel Solorzano Barruso, Instituto de Salud Carlos III, Universidad de Salamanca, Consejo Superior de Investigaciones Científicas (España), Instituto de Investigación Biomédica de Salamanca, Landeira-Viñuela, Alicia, Arias-Hidalgo, Carlota, Juanes-Velasco, Pablo, Alcoceba, Miguel, Navarro-Bailón, Almudena, Pedreira, C. E., Lécrevisse, Quentin, Díaz-Muñoz, Laura, Sanchez-Santos, Jose Manuel, Hernández, Ángela-Patricia, García-Vaquero, Marina L., Góngora, Rafael, De Las Rivas, Javier, González, Marcos, Orfao, Alberto, and Fuentes, Manuel
- Abstract
Chronic lymphocytic leukemia (CLL) is a lymphoid neoplasm characterized by the accumulation of mature B cells. The diagnosis is established by the detection of monoclonal B lymphocytes in peripheral blood, even in early stages [monoclonal B-cell lymphocytosis (MBL)], and its clinical course is highly heterogeneous. In fact, there are well-characterized multiple prognostic factors that are also related to the observed genetic heterogenicity, such as immunoglobulin heavy chain variable region (IGHV) mutational status, del17p, and TP53 mutations, among others. Moreover, a dysregulation of the immune system (innate and adaptive immunity) has been observed in CLL patients, with strong impact on immune surveillance and consequently on the onset, evolution, and therapy response. In addition, the tumor microenvironment is highly complex and heterogeneous (i.e., matrix, fibroblast, endothelial cells, and immune cells), playing a critical role in the evolution of CLL. In this study, a quantitative profile of 103 proteins (cytokines, chemokines, growth/regulatory factors, immune checkpoints, and soluble receptors) in 67 serum samples (57 CLL and 10 MBL) has been systematically evaluated. Also, differential profiles of soluble immune factors that discriminate between MBL and CLL (sCD47, sCD27, sTIMD-4, sIL-2R, and sULBP-1), disease progression (sCD48, sCD27, sArginase-1, sLAG-3, IL-4, and sIL-2R), or among profiles correlated with other prognostic factors, such as IGHV mutational status (CXCL11/I-TAC, CXCL10/IP-10, sHEVM, and sLAG-3), were deciphered. These results pave the way to explore the role of soluble immune checkpoints as a promising source of biomarkers in CLL, to provide novel insights into the immune suppression process and/or dysfunction, mostly on T cells, in combination with cellular balance disruption and microenvironment polarization leading to tumor escape.
- Published
- 2022
23. Deciphering biomarkers for leptomeningeal metastasis in malignant hemopathies (Lymphoma/Leukemia) patients by comprehensive multipronged proteomics characterization of cerebrospinal fluid
- Author
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Instituto de Salud Carlos III, European Commission, Junta de Castilla y León, Consejo Nacional de Ciencia y Tecnología (México), Juanes-Velasco, Pablo, Galicia, N., Pin, Elisa, Jara-Acevedo, Ricardo, Carabias-Sánchez, Javier, García-Valiente, R., Lécrevisse, Quentin, Pedreira, C. E., Góngora, Rafael, Sanchez-Santos, Jose Manuel, Lorenzo-Gil, Héctor, Landeira-Viñuela, Alicia, Bareke, Halin, Orfao, Alberto, Nilsson, Peter, Fuentes, Manuel, Instituto de Salud Carlos III, European Commission, Junta de Castilla y León, Consejo Nacional de Ciencia y Tecnología (México), Juanes-Velasco, Pablo, Galicia, N., Pin, Elisa, Jara-Acevedo, Ricardo, Carabias-Sánchez, Javier, García-Valiente, R., Lécrevisse, Quentin, Pedreira, C. E., Góngora, Rafael, Sanchez-Santos, Jose Manuel, Lorenzo-Gil, Héctor, Landeira-Viñuela, Alicia, Bareke, Halin, Orfao, Alberto, Nilsson, Peter, and Fuentes, Manuel
- Abstract
In the present work, leptomeningeal disease, a very destructive form of systemic cancer, was characterized from several proteomics points of view. This pathology involves the invasion of the leptomeninges by malignant tumor cells. The tumor spreads to the central nervous system through the cerebrospinal fluid (CSF) and has a very grim prognosis; the average life expectancy of patients who suffer it does not exceed 3 months. The early diagnosis of leptomeningeal disease is a challenge because, in most of the cases, it is an asymptomatic pathology. When the symptoms are clear, the disease is already in the very advanced stages and life expectancy is low. Consequently, there is a pressing need to determine useful CSF proteins to help in the diagnosis and/or prognosis of this disease. For this purpose, a systematic and exhaustive proteomics characterization of CSF by multipronged proteomics approaches was performed to determine different protein profiles as potential biomarkers. Proteins such as PTPRC, SERPINC1, sCD44, sCD14, ANPEP, SPP1, FCGR1A, C9, sCD19, and sCD34, among others, and their functional analysis, reveals that most of them are linked to the pathology and are not detected on normal CSF. Finally, a panel of biomarkers was verified by a prediction model for leptomeningeal disease, showing new insights into the research for potential biomarkers that are easy to translate into the clinic for the diagnosis of this devastating disease.
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- 2022
24. Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study
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European Commission, European Hematology Association, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministry of Health of the Czech Republic, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (España), Brouwer, Nienke, Matarraz, Sergio, Nierkens, Stefan, Hofmans, Mattias, Novákova, Michaela, Sobral da Costa, E., Fernández, Paula, Bras, A. E., Vieira de Mello, Fabiana, Mejstrikova, Ester, Philippé, J., Grigore, Georgiana Emilia, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, Velden, Vincent H. J. van der, European Commission, European Hematology Association, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministry of Health of the Czech Republic, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (España), Brouwer, Nienke, Matarraz, Sergio, Nierkens, Stefan, Hofmans, Mattias, Novákova, Michaela, Sobral da Costa, E., Fernández, Paula, Bras, A. E., Vieira de Mello, Fabiana, Mejstrikova, Ester, Philippé, J., Grigore, Georgiana Emilia, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, and Velden, Vincent H. J. van der
- Abstract
Acute megakaryoblastic leukemia (AMKL) is a rare and heterogeneous subtype of acute myeloid leukemia (AML). We evaluated the immunophenotypic profile of 72 AMKL and 114 non-AMKL AML patients using the EuroFlow AML panel. Univariate and multivariate/multidimensional analyses were performed to identify most relevant markers contributing to the diagnosis of AMKL. AMKL patients were subdivided into transient abnormal myelopoiesis (TAM), myeloid leukemia associated with Down syndrome (ML-DS), AML—not otherwise specified with megakaryocytic differentiation (NOS-AMKL), and AMKL—other patients (AML patients with other WHO classification but with flowcytometric features of megakaryocytic differentiation). Flowcytometric analysis showed good discrimination between AMKL and non-AMKL patients based on differential expression of, in particular, CD42a.CD61, CD41, CD42b, HLADR, CD15 and CD13. Combining CD42a.CD61 (positive) and CD13 (negative) resulted in a sensitivity of 71% and a specificity of 99%. Within AMKL patients, TAM and ML-DS patients showed higher frequencies of immature CD34+/CD117+ leukemic cells as compared to NOS-AMKL and AMKL-Other patients. In addition, ML-DS patients showed a significantly higher expression of CD33, CD11b, CD38 and CD7 as compared to the other three subgroups, allowing for good distinction of these patients. Overall, our data show that the EuroFlow AML panel allows for straightforward diagnosis of AMKL and that ML-DS is associated with a unique immunophenotypic profile.
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- 2022
25. Expert-independent classification of mature B-cell neoplasms using standardized flow cytometry: a multicentric study
- Author
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European Commission, European Hematology Association, Böttcher, Sebastian, Engelmann, Robby, Grigore, Georgiana Emilia, Fernández, Paula, Caetano, J., Flores-Montero, Juan, Velden, Vincent H. J. van der, Novákova, Michaela, Philippé, J., Ritgen, Matthias, Burgos, Leire, Lécrevisse, Quentin, Lange, Sandra, Kalina, Tomas, Verde, Javier, Fluxá, Rafael, Dongen, J. J. M. van, Pedreira, C. E., Orfao, Alberto, European Commission, European Hematology Association, Böttcher, Sebastian, Engelmann, Robby, Grigore, Georgiana Emilia, Fernández, Paula, Caetano, J., Flores-Montero, Juan, Velden, Vincent H. J. van der, Novákova, Michaela, Philippé, J., Ritgen, Matthias, Burgos, Leire, Lécrevisse, Quentin, Lange, Sandra, Kalina, Tomas, Verde, Javier, Fluxá, Rafael, Dongen, J. J. M. van, Pedreira, C. E., and Orfao, Alberto
- Abstract
Reproducible expert-independent flow-cytometric criteria for the differential diagnoses between mature B-cell neoplasms are lacking. We developed an algorithm-driven classification for these lymphomas by flow cytometry and compared it to the WHO gold standard diagnosis. Overall, 662 samples from 662 patients representing 9 disease categories were analyzed at 9 laboratories using the previously published EuroFlow 5-tube-8-color B-cell chronic lymphoproliferative disease antibody panel. Expression levels of all 26 markers from the panel were plotted by B-cell entity to construct a univariate, fully standardized diagnostic reference library. For multivariate data analysis, we subsequently used canonical correlation analysis of 176 training cases to project the multidimensional space of all 26 immunophenotypic parameters into 36 2-dimensional plots for each possible pairwise differential diagnosis. Diagnostic boundaries were fitted according to the distribution of the immunophenotypes of a given differential diagnosis. A diagnostic algorithm based on these projections was developed and subsequently validated using 486 independent cases. Negative predictive values exceeding 92.1% were observed for all disease categories except for follicular lymphoma. Particularly high positive predictive values were returned in chronic lymphocytic leukemia (99.1%), hairy cell leukemia (97.2%), follicular lymphoma (97.2%), and mantle cell lymphoma (95.4%). Burkitt and CD10 diffuse large B-cell lymphomas were difficult to distinguish by the algorithm. A similar ambiguity was observed between marginal zone, lymphoplasmacytic, and CD10 diffuse large B-cell lymphomas. The specificity of the approach exceeded 98% for all entities. The univariate immunophenotypic library and the multivariate expert-independent diagnostic algorithm might contribute to increased reproducibility of future diagnostics in mature B-cell neoplasms.
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- 2022
26. Missing Data Analysis in Regression
- Author
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Marcelino, C. G., primary, Leite, G. M. C., additional, Celes, P., additional, and Pedreira, C. E., additional
- Published
- 2022
- Full Text
- View/download PDF
27. Representatividad del Registro de Tumores Torácicos de España. Comparación de datos sociodemográficos con otros registros nacionales
- Author
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Candal-Pedreira C, Ruano-Ravina A, Carcereny E, Rodríguez-Abreu D, Guirado-Risueño M, López-Castro R, Massutí B, Blasco A, Ortega AL, and Provencio M
- Subjects
Age ,Cáncer de pulmón ,Spain ,Registro de tumores ,España ,Edad ,Sex ,Cancer registry ,Lung cancer ,Sexo - Abstract
OBJECTIVE: In Spain, due to the lack of data at national level a lung cancer registry, the Thoracic Tumour Registry (TTR), was created. Such registry should demonstrate comparability with population-based data to ensure representativeness at population level. The aim is to compare the socio-demographic characteristics of the TTR with incidence data from the Red de Registros de Cáncer (REDECAN) and mortality data from the Instituto Nacional de Estadística (INE). METHOD: Lung cancer data sources available to date, REDECAN and INE, were used. Lung cancer cases overall and disaggregated by sex and age groups were collected from each source of information and data were compared for the period 2017-2020. Sex and age group proportions of TTR were calculated for both databases (which collect incidence and mortality data), for the entire study period and broken down by year. RESULTS: A total of 17,109 incident lung cancer cases from the TTR, 58,668 estimated incident cases from REDECAN and 88,083 deaths registered from INE between 2017 and 2020 were included. In terms of sex, the proportions are very similar between the three sources and the differences do not exceed 4%. In terms of age, the differences are not large, being larger for mortality data in the older age group from the INE versus the TTR. CONCLUSIONS: The TTR seems to be representative of lung cancer cases diagnosed in Spain between 2019 and 2020, both by sex and age. This allows us to accurately characterise the status of this disease, which is the leading cause of cancer death in Spain, and that the analysis of results obtained from the RTT can be applied to cases of lung cancer diagnosed in our country.
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- 2022
28. Should the European Union have an office of research integrity?
- Author
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Candal-Pedreira, C, Ruano-Ravina, A, and Pérez-Ríos, M
- Published
- 2021
- Full Text
- View/download PDF
29. A model for antibiotic therapy: Tecidual kinetics implications
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Pedreira, C. E., Muniz, P. S., Mello, M. A., Thoma, M., editor, Wyner, A., editor, Sebastian, H. -J., editor, and Tammer, K., editor
- Published
- 1990
- Full Text
- View/download PDF
30. Low-beta repetitive transcranial magnetic stimulation to human dorsolateral prefrontal cortex during object recognition memory sample presentation, at a task-related frequency observed in local field potentials in homologous macaque cortex, impairs subsequent recollection but not familiarity
- Author
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Wu, Z., Kavanová, M., Hickman, L.J., Boschin, E.A., Galeazzi, J.M., Verhagen, L., Ainsworth, M., Pedreira, C., Buckley, M.J., Wu, Z., Kavanová, M., Hickman, L.J., Boschin, E.A., Galeazzi, J.M., Verhagen, L., Ainsworth, M., Pedreira, C., and Buckley, M.J.
- Abstract
Contains fulltext : 241277.pdf (Publisher’s version ) (Open Access), According to dual-process signal-detection (DPSD) theories, short- and long-term recognition memory draws upon both familiarity and recollection. It remains unclear how primate prefrontal cortex (PFC) contributes to these processes, but frequency-specific neuronal activities are considered to play a key role. In Experiment 1, nonhuman primate (NHP) local field potential (LFP) electrophysiological recordings in macaque left dorsolateral PFC (dlPFC) revealed performance-related differences in a low-beta frequency range during the sample presentation phase of a visual object recognition memory task. Experiment 2 employed a similar task in humans and targeted left dlPFC (and vertex as a control) with repetitive transcranial magnetic stimulation (rTMS) at 12.5 Hz during occasional sample presentations. This low-beta frequency rTMS to dlPFC decreased DPSD derived indices of recollection, but not familiarity, in subsequent memory tests of the targeted samples after short delays. The same number of rTMS pulses over the same total duration albeit at a random frequency had no effect on either recollection or familiarity. Neither stimulation protocols had any causal effect upon behaviour when targeted to the control site (vertex). In this study, our hypotheses for our human TMS study were derived from our observations in NHPs; this approach might inspire further translational research through investigation of homologous brain regions and tasks across species using similar neuroscientific methodologies to advance the neural mechanism of recognition memory in primates.
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- 2021
31. Flow cytometry immunophenotyping for diagnostic orientation and classification of pediatric cancer based on the euroflow solid tumor orientation tube (Stot)
- Author
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Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Chevron, European Commission, Centro de Investigación Biomédica en Red Cáncer (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundaçao Capes (Brasil), Ministerio de Educación, Cultura y Deporte (España), Ferreira-Facio, Cristiane S., Botafogo, Vitor, Mello Ferrão, Patrícia, Canellas, María Clara, Milito, Cristiane B., Romano, Sérgio, Lopes, Daiana V., Teixeira, Lisandra C., Oliveira, Elen, Bruno-Riscarolli, Enrico, Mello, Fabiana V., Siqueira, Patrícia F. R., Moura, Patrícia, Nicanor Macedo, Francisco, Forny, Danielle N., Simião, Luíza, Pureza, Ana Luíza, Poirot Land, Marcelo Gerardin, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, Sobral da Costa, E., Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Chevron, European Commission, Centro de Investigación Biomédica en Red Cáncer (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundaçao Capes (Brasil), Ministerio de Educación, Cultura y Deporte (España), Ferreira-Facio, Cristiane S., Botafogo, Vitor, Mello Ferrão, Patrícia, Canellas, María Clara, Milito, Cristiane B., Romano, Sérgio, Lopes, Daiana V., Teixeira, Lisandra C., Oliveira, Elen, Bruno-Riscarolli, Enrico, Mello, Fabiana V., Siqueira, Patrícia F. R., Moura, Patrícia, Nicanor Macedo, Francisco, Forny, Danielle N., Simião, Luíza, Pureza, Ana Luíza, Poirot Land, Marcelo Gerardin, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, and Sobral da Costa, E.
- Abstract
Early diagnosis of pediatric cancer is key for adequate patient management and improved outcome. Although multiparameter flow cytometry (MFC) has proven of great utility in the diagnosis and classification of hematologic malignancies, its application to non-hematopoietic pediatric tumors remains limited. Here we designed and prospectively validated a new single eight-color antibody combination—solid tumor orientation tube, STOT—for diagnostic screening of pediatric cancer by MFC. A total of 476 samples (139 tumor mass, 138 bone marrow, 86 lymph node, 58 peripheral blood, and 55 other body fluid samples) from 296 patients with diagnostic suspicion of pediatric cancer were analyzed by MFC vs. conventional diagnostic procedures. STOT was designed after several design–test–evaluate–redesign cycles based on a large panel of monoclonal antibody combinations tested on 301 samples. In its final version, STOT consists of a single 8-color/12-marker antibody combination (CD99-CD8/numyogenin/CD4-EpCAM/CD56/GD2/smCD3-CD19/cyCD3-CD271/CD45). Prospective validation of STOT in 149 samples showed concordant results with the patient WHO/ICCC-3 diagnosis in 138/149 cases (92.6%). These included: 63/63 (100%) reactive/disease-free samples, 43/44 (98%) malignant and 4/4 (100%) benign non-hematopoietic tumors together with 28/38 (74%) leukemia/lymphoma cases; the only exception was Hodgkin lymphoma that required additional markers to be stained. In addition, STOT allowed accurate discrimination among the four most common subtypes of malignant CD45− CD56++ non-hematopoietic solid tumors: 13/13 (GD2++ numyogenin− CD271−/+ nuMyoD1− CD99− EpCAM−) neuroblastoma samples, 5/5 (GD2− numyogenin++ CD271++ nuMyoD1++ CD99−/+ EpCAM−) rhabdomyosarcomas, 2/2 (GD2−/+ numyogenin− CD271+ nuMyoD1− CD99+ EpCAM−) Ewing sarcoma family of tumors, and 7/7 (GD2− numyogenin− CD271+ nuMyoD1− CD99− EpCAM+) Wilms tumors. In summary, here we designed and validated a new standardized antibody combination and MFC assay
- Published
- 2021
32. Tracking the antibody immunome in sporadic colorectal cancer by using antigen self-assembled protein arrays
- Author
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Instituto de Salud Carlos III, European Commission, Junta de Castilla y León, Fundación Memoria de D. Samuel Solorzano Barruso, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Universidad de Salamanca, González-González, María, Sayagués, José María, Muñoz-Bellvis, Luís, Pedreira, C. E., Campos, Marcello L. R. de, García, Jacinto, Alcazar, Jose Antonio, Braz, Patrick F., Galves, Breno L., González, Luis Miguel, Bengoechea, Óscar, Abad, María del Mar, Cruz, Juan Jesús, Bellido, Lorena, Fonseca, Emilio, Díez, Paula, Juanes-Velasco, Pablo, Landeira-Viñuela, Alicia, Lécrevisse, Quentin, Montalvillo, Enrique, Góngora, Rafael, Blanco, Óscar, Sanchez-Santos, Jose Manuel, LaBaer, Joshua, Orfao, Alberto, Fuentes, Manuel, Instituto de Salud Carlos III, European Commission, Junta de Castilla y León, Fundación Memoria de D. Samuel Solorzano Barruso, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Universidad de Salamanca, González-González, María, Sayagués, José María, Muñoz-Bellvis, Luís, Pedreira, C. E., Campos, Marcello L. R. de, García, Jacinto, Alcazar, Jose Antonio, Braz, Patrick F., Galves, Breno L., González, Luis Miguel, Bengoechea, Óscar, Abad, María del Mar, Cruz, Juan Jesús, Bellido, Lorena, Fonseca, Emilio, Díez, Paula, Juanes-Velasco, Pablo, Landeira-Viñuela, Alicia, Lécrevisse, Quentin, Montalvillo, Enrique, Góngora, Rafael, Blanco, Óscar, Sanchez-Santos, Jose Manuel, LaBaer, Joshua, Orfao, Alberto, and Fuentes, Manuel
- Abstract
Sporadic Colorectal Cancer (sCRC) is the third leading cause of cancer death in the Western world, and the sCRC patients presenting with synchronic metastasis have the poorest prognosis. Genetic alterations accumulated in sCRC tumor cells translate into mutated proteins and/or abnormal protein expression levels, which contribute to the development of sCRC. Then, the tumor-associated proteins (TAAs) might induce the production of auto-antibodies (aAb) via humoral immune response. Here, Nucleic Acid Programmable Protein Arrays (NAPPArray) are employed to identify aAb in plasma samples from a set of 50 sCRC patients compared to seven healthy donors. Our goal was to establish a systematic workflow based on NAPPArray to define differential aAb profiles between healthy individuals and sCRC patients as well as between non-metastatic (n = 38) and metastatic (n = 12) sCRC, in order to gain insight into the role of the humoral immune system in controlling the development and progression of sCRC. Our results showed aAb profile based on 141 TAA including TAAs associated with biological cellular processes altered in genesis and progress of sCRC (e.g., FSCN1, VTI2 and RPS28) that discriminated healthy donors vs. sCRC patients. In addition, the potential capacity of discrimination (between non-metastatic vs. metastatic sCRC) of 7 TAAs (USP5, ML4, MARCKSL1, CKMT1B, HMOX2, VTI2, TP53) have been analyzed individually in an independent cohort of sCRC patients, where two of them (VTI2 and TP53) were validated (AUC ~75%). In turn, these findings provided novel insights into the immunome of sCRC, in combination with transcriptomics profiles and protein antigenicity characterizations, wich might lead to the identification of novel sCRC biomarkers that might be of clinical utility for early diagnosis of the tumor. These results explore the immunomic analysis as potent source for biomarkers with diagnostic and prognostic value in CRC. Additional prospective studies in larger series of patie
- Published
- 2021
33. CLL-like B-lymphocytes are systematically present at very low numbers in peripheral blood of healthy adults
- Author
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Almeida, J, Nieto, W G, Teodosio, C, Pedreira, C E, López, A, Fernández-Navarro, P, Nieto, A, Rodríguez-Caballero, A, Muñoz-Criado, S, Jara-Acevedo, M, Romero, A, and Orfao, A
- Published
- 2011
- Full Text
- View/download PDF
34. Classification of EEG abnormalities in partial epilepsy with simultaneous EEG–fMRI recordings
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Pedreira, C., Vaudano, A. E., Thornton, R. C., Chaudhary, U. J., Vulliemoz, S., Laufs, H., Rodionov, R., Carmichael, D. W., Lhatoo, S. D., Guye, M., Quiroga, Quian R., and Lemieux, L.
- Published
- 2014
- Full Text
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35. Seasonal herbage accumulation, plant-part composition and nutritive value of signal grass (Urochloa decumbens) pastures under simulated continuous stocking
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BRAGA, G. J., PEDREIRA, C. G. S., FERREIRA, A. S., OLIVEIRA, E. A. de, PAULINO, V. T., GUSTAVO JOSE BRAGA, CPAC, CARLOS GUILHERME SILVEIRA PEDREIRA, ALIEDSON SAMPAIO FERREIRA, ELIARA ANAÍ DE OLIVEIRA, and VALDINEI TADEU PAULINO.
- Subjects
Pastagem ,Capim ,Forragem ,Digestibilidade ,Proteína Bruta - Abstract
Made available in DSpace on 2020-09-17T04:38:40Z (GMT). No. of bitstreams: 1 Gustavo-Seasonal-herbage.pdf: 677000 bytes, checksum: 89a6e4afe2daad49aa63e9e6fa4528e6 (MD5) Previous issue date: 2020
- Published
- 2020
36. A new automated flow cytometry data analysis approach for the diagnostic screening of neoplastic B-cell disorders in peripheral blood samples with absolute lymphocytosis
- Author
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Costa, E S, Arroyo, M E, Pedreira, C E, García-Marcos, M A, Tabernero, M D, Almeida, J, and Orfao, A
- Published
- 2006
- Full Text
- View/download PDF
37. Age distribution of multiple functionally relevant subsets of CD4+ T cells in human blood using a standardized and validated 14-color EuroFlow immune monitoring tube
- Author
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Innovative Medicines Initiative, European Commission, European Federation of Pharmaceutical Industries and Associations, Bill & Melinda Gates Foundation, Instituto de Salud Carlos III, Fundación Mutua Madrileña, Ministerio de Ciencia, Innovación y Universidades (España), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministério da Ciência, Tecnologia e Inovação (Brasil), Agencia Estatal de Investigación (España), Botafogo, Vitor, Pérez-Andrés, Martin, Jara-Acevedo, Maria, Bárcena, Paloma, Grigore, Georgiana Emilia, Hernández-Delgado, Alejandro, Damasceno, Daniela, Comans, Suzanne, Blanco, Elena, Romero, Alfonso, Arriba-Méndez, Sonia, Gastaca, Irene, Pedreira, C. E., Gaans-van den Brink, Jacqueline A. M. van, Corbiere, Véronique, Mascart, Françoise, Els, Cécile A. C. M. van, Barkoff, Alex-Mikael, Mayado, Andrea, Dongen, J. J. M. van, Almeida, Julia, Orfao, Alberto, Innovative Medicines Initiative, European Commission, European Federation of Pharmaceutical Industries and Associations, Bill & Melinda Gates Foundation, Instituto de Salud Carlos III, Fundación Mutua Madrileña, Ministerio de Ciencia, Innovación y Universidades (España), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministério da Ciência, Tecnologia e Inovação (Brasil), Agencia Estatal de Investigación (España), Botafogo, Vitor, Pérez-Andrés, Martin, Jara-Acevedo, Maria, Bárcena, Paloma, Grigore, Georgiana Emilia, Hernández-Delgado, Alejandro, Damasceno, Daniela, Comans, Suzanne, Blanco, Elena, Romero, Alfonso, Arriba-Méndez, Sonia, Gastaca, Irene, Pedreira, C. E., Gaans-van den Brink, Jacqueline A. M. van, Corbiere, Véronique, Mascart, Françoise, Els, Cécile A. C. M. van, Barkoff, Alex-Mikael, Mayado, Andrea, Dongen, J. J. M. van, Almeida, Julia, and Orfao, Alberto
- Abstract
CD4+ T cells comprise multiple functionally distinct cell populations that play a key role in immunity. Despite blood monitoring of CD4+ T-cell subsets is of potential clinical utility, no standardized and validated approaches have been proposed so far. The aim of this study was to design and validate a single 14-color antibody combination for sensitive and reproducible flow cytometry monitoring of CD4+ T-cell populations in human blood to establish normal age-related reference values and evaluate the presence of potentially altered profiles in three distinct disease models—monoclonal B-cell lymphocytosis (MBL), systemic mastocytosis (SM), and common variable immunodeficiency (CVID). Overall, 145 blood samples from healthy donors were used to design and validate a 14-color antibody combination based on extensive reagent testing in multiple cycles of design–testing–evaluation–redesign, combined with in vitro functional studies, gene expression profiling, and multicentric evaluation of manual vs. automated gating. Fifteen cord blood and 98 blood samples from healthy donors (aged 0–89 years) were used to establish reference values, and another 25 blood samples were evaluated for detecting potentially altered CD4 T-cell subset profiles in MBL (n = 8), SM (n = 7), and CVID (n = 10). The 14-color tube can identify ≥89 different CD4+ T-cell populations in blood, as validated with high multicenter reproducibility, particularly when software-guided automated (vs. manual expert-based) gating was used. Furthermore, age-related reference values were established, which reflect different kinetics for distinct subsets: progressive increase of naïve T cells, T-helper (Th)1, Th17, follicular helper T (TFH) cells, and regulatory T cells (Tregs) from birth until 2 years, followed by a decrease of naïve T cells, Th2, and Tregs in older children and a subsequent increase in multiple Th-cell subsets toward late adulthood. Altered and unique CD4+ T-cell subset profiles were detected in tw
- Published
- 2020
38. Automated identification of leukocyte subsets improves standardization of database-guided expert-supervised diagnostic orientation in acute leukemia: a EuroFlow study
- Author
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European Commission, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Silesian University of Technology, Lhermitte, Ludovic, Barreau, Sylvain, Morf, Daniela, Fernández, Paula, Grigore, Georgiana Emilia, Barrena, Susana, Bie, Maaike de, Flores-Montero, Juan, Brüggemann, Monika, Mejstrikova, Ester, Nierkens, Stefan, Burgos, Leire, Caetano, J., Gaipa, Giuseppe, Buracchi, Chiara, Sobral da Costa, E., Sedek, Lukasz, Szczepanski, Tomasz, Aanei, Carmen-Mariana, Sluijs-Gelling, Alita J. van der, Hernández, Alejandro, Fluxá, Rafael, Lécrevisse, Quentin, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, Velden, Vincent H. J. van der, European Commission, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Silesian University of Technology, Lhermitte, Ludovic, Barreau, Sylvain, Morf, Daniela, Fernández, Paula, Grigore, Georgiana Emilia, Barrena, Susana, Bie, Maaike de, Flores-Montero, Juan, Brüggemann, Monika, Mejstrikova, Ester, Nierkens, Stefan, Burgos, Leire, Caetano, J., Gaipa, Giuseppe, Buracchi, Chiara, Sobral da Costa, E., Sedek, Lukasz, Szczepanski, Tomasz, Aanei, Carmen-Mariana, Sluijs-Gelling, Alita J. van der, Hernández, Alejandro, Fluxá, Rafael, Lécrevisse, Quentin, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, and Velden, Vincent H. J. van der
- Abstract
Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant classification panel and final diagnosis. In this study, we designed and validated an algorithm for automated (database-supported) gating and identification (AGI tool) of cell subsets within samples stained with ALOT. A reference database of normal peripheral blood (PB, n = 41) and bone marrow (BM; n = 45) samples analyzed with the ALOT was constructed, and served as a reference for the AGI tool to automatically identify normal cells. Populations not unequivocally identified as normal cells were labeled as checks and were classified by an expert. Additional normal BM (n = 25) and PB (n = 43) and leukemic samples (n = 109), analyzed in parallel by experts and the AGI tool, were used to evaluate the AGI tool. Analysis of normal PB and BM samples showed low percentages of checks (<3% in PB, <10% in BM), with variations between different laboratories. Manual analysis and AGI analysis of normal and leukemic samples showed high levels of correlation between cell numbers (r2 > 0.95 for all cell types in PB and r2 > 0.75 in BM) and resulted in highly concordant classification of leukemic cells by our previously published automated database-guided expert-supervised orientation tool for immunophenotypic diagnosis and classification of acute leukemia (Compass tool). Similar data were obtained using alternative, commercially available tubes, confirming the robustness of the developed tools. The AGI tool represents an innovative step in minimizing human intervention and requirements in expertise, toward a “sample-in and result-out” approach which may result in more objective and reproducible data analysis and diagnostics. The AGI tool may improve quality of immunophenotyping in individual laboratories, since high percentages of checks in normal samples are an alert on the quality of the internal procedures.
- Published
- 2020
39. An architecture for computing Zech's logarithms in GF(2m)
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Assis, F and Pedreira, C
- Subjects
Computer architecture -- Research ,Logarithms -- Research ,Computer programming -- Research - Published
- 2000
40. Automated pattern-guided principal component analysis vs expert-based immunophenotypic classification of B-cell chronic lymphoproliferative disorders: a step forward in the standardization of clinical immunophenotyping
- Author
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Costa, E S, Pedreira, C E, Barrena, S, Lecrevisse, Q, Flores, J, Quijano, S, Almeida, J, del Carmen García-Macias, M, Bottcher, S, Van Dongen, J JM, and Orfao, A
- Published
- 2010
- Full Text
- View/download PDF
41. P1.01-07 Asbestos Exposure and Small Cell Lung Cancer. Systematic Review and Meta-Analysis.
- Author
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Rey Brandariz, J., Ruano-Ravina, A., Curiel-García, T., Varela-Lema, L., Candal Pedreira, C., Mourino, N., and Pérez Ríos, M.
- Published
- 2023
- Full Text
- View/download PDF
42. Health-care problems of Turner syndrome in the adult woman: a cross sectional study of a Victorian cohort and a case for transition
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Pedreira, C. C., Hameed, R., Kanumakala, S., and Zacharin, M.
- Published
- 2006
43. Age-Related Pituitary Volumes in Prepubertal Children with Normal Endocrine Function: Volumetric Magnetic Resonance Data
- Author
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Fink, A M., Vidmar, S, Kumbla, S, Pedreira, C C., Kanumakala, S, Williams, C, Carlin, J B., and Cameron, F J.
- Published
- 2005
44. Fasting Ghrelin Levels Are Not Elevated in Children with Hypothalamic Obesity
- Author
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Kanumakala, S, Greaves, R, Pedreira, C C., Donath, S, Warne, G L., Zacharin, M R., and Harris, M
- Published
- 2005
45. Association of Body Composition and Lung Function in Children With Cystic Fibrosis
- Author
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Pedreira, C. C., Robert, R. G.D., Dalton, V., Oliver, M. R., Carlin, J. B., Robinson, P., and Cameron, F. J.
- Published
- 2005
- Full Text
- View/download PDF
46. Lot-to-lot stability of antibody reagents for flow cytometry
- Author
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Böttcher, Sebastian, Velden, Vincent H. J. van der, Villamor, Neus, Ritgen, Matthias, Flores-Montero, Juan, Murua Escobar, Hugo, Kalina, Tomas, Brüggemann, Monika, Grigore, Georgiana Emilia, Martín-Ayuso, Marta, Lécrevisse, Quentin, Pedreira, C. E., Dongen, J. J. M. van, Orfao, Alberto, Böttcher, Sebastian, Velden, Vincent H. J. van der, Villamor, Neus, Ritgen, Matthias, Flores-Montero, Juan, Murua Escobar, Hugo, Kalina, Tomas, Brüggemann, Monika, Grigore, Georgiana Emilia, Martín-Ayuso, Marta, Lécrevisse, Quentin, Pedreira, C. E., Dongen, J. J. M. van, and Orfao, Alberto
- Abstract
The fluorescence detected using fluorochrome-labelled monoclonal antibodies depends not only on the abundance of the target antigen, but amongst many other factors also on the effective fluorochrome-to-antibody ratio. The diagnostic approach of the EuroFlow consortium relies on reproducible fluorescence intensities over time. A capture bead system for mouse immunoglobulin light chains was utilized to compare the mean fluorescence intensity of 1323 consecutive antibody lots to the currently used lot of the same monoclonal antibody. In total, 157 different monoclonal antibodies were assessed over seven years. Median relative difference between consecutive lots was 3.8% (range: 0.01% to 164.7%, interquartile range: 1.3% to 10.1%). The relative difference exceeded 20% in 8.8% of all comparisons. FITC labelled monoclonal antibodies (median relative difference: 2.1%) showed a significantly smaller variation between lots than antibodies conjugated to PE (3.5%), PECy7 (3.9%), PerCPCy5.5 (5.8%), APC (5.8%), APCH7 (7.4%), and APCC750 (14.5%). Reagents labelled with Pacific Blue (1.4%), Pacific Orange (2.4%), HV450 (0.7%), and HV500 (1.7%) demonstrated more consistent results compared to conjugates of BV421 (4.1%) and BV510 (16.2%). Additionally, significant differences in lot-to-lot fluorescence stability amongst antibodies labelled with the same fluorochrome were observed between manufacturers. These observations might guide future quality recommendations for the production and application of fluorescence-labelled monoclonal antibodies in multicolor flow cytometry.
- Published
- 2019
47. From big flow cytometry datasets to smart diagnostic strategies: The EuroFlow approach
- Author
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Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Fundações de Amparo à Pesquisa (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Pedreira, C. E., Sobral da Costa, E., Lécrevisse, Quentin, Grigore, Georgiana Emilia, Fluxá, Rafael, Verde, Javier, Hernández, Juan, Dongen, J. J. M. van, Orfao, Alberto, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Fundações de Amparo à Pesquisa (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Pedreira, C. E., Sobral da Costa, E., Lécrevisse, Quentin, Grigore, Georgiana Emilia, Fluxá, Rafael, Verde, Javier, Hernández, Juan, Dongen, J. J. M. van, and Orfao, Alberto
- Abstract
The rise in the analytical speed of mutiparameter flow cytometers made possible by the introduction of digital instruments, has brought up the possibility to manage progressively higher number of parameters simultaneously on significantly greater numbers of individual cells. This has led to an exponential increase in the complexity and volume of flow cytometry data generated about cells present in individual samples evaluated in a single measurement. This increase demands for new developments in flow cytometry data analysis, graphical representation, and visualization and interpretation tools to address the new big data challenges, i.e. processing data files of ≥10–25 parameters per cell in samples with >5–10 million cells (= up to 250 million data points per cell sample) obtained in a few minutes. Here, we present a comprehensive review of some of the tools developed by the EuroFlow consortium for processing flow cytometric big data files in diagnostic laboratories, particularly focused on automated EuroFlow approaches for: i) identification of all cell populations coexisting in a sample (automated gating); ii) smart classification of aberrant cell populations in routine diagnostics; iii) automated reporting; together with iv) new tools developed to visualize n-dimensional data in 2-dimensional plots to support expert-guided automated data analysis. The concept of using reference data bases implemented into software programs, in combination with multivariate statistical analysis pioneered by EuroFlow, provides an innovative, highly efficient and fast approach for diagnostic screening, classification and monitoring of patients with distinct hematological and immune disorders, as well as other diseases.
- Published
- 2019
48. Maturation-associated gene expression profiles during normal human bone marrow erythropoiesis
- Author
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministerio de Educación, Cultura y Deporte (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), European Commission, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundaçao Capes (Brasil), Mello, Fabiana V., Land, Marcelo G. P., Costa, Elaine S., Teodosio, Cristina, Sánchez, Maria Luz, Bárcena, Paloma, Peres, Rodrigo T., Pedreira, C. E., Alves, Liliane R., Orfao, Alberto, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministerio de Educación, Cultura y Deporte (España), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), European Commission, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundaçao Capes (Brasil), Mello, Fabiana V., Land, Marcelo G. P., Costa, Elaine S., Teodosio, Cristina, Sánchez, Maria Luz, Bárcena, Paloma, Peres, Rodrigo T., Pedreira, C. E., Alves, Liliane R., and Orfao, Alberto
- Abstract
Erythropoiesis has been extensively studied using in vitro and in vivo animal models. Despite this, there is still limited data about the gene expression profiles (GEP) of primary (ex vivo) normal human bone marrow (BM) erythroid maturation. We investigated the GEP of nucleated red blood cell (NRBC) precursors during normal human BM erythropoiesis. Three maturation-associated populations of NRBC were identified and purified from (fresh) normal human BM by flow cytometry and the GEP of each purified cell population directly analyzed using DNA-oligonucleotide microarrays. Overall, 6569 genes (19% of the genes investigated) were expressed in ≥1 stage of BM erythropoiesis at stable (e.g., genes involved in DNA process, cell signaling, protein organization and hemoglobin production) or variable amounts (e.g., genes related to cell differentiation, apoptosis, metabolism), the latter showing a tendency to either decrease from stage 1 to 3 (genes associated with regulation of erythroid differentiation and survival, e.g., SPI1, STAT5A) or increase from stage 2 to stage 3 (genes associated with autophagy, erythroid functions such as heme production, e.g., ALAS1, ALAS2), iron metabolism (e.g., ISCA1, SLC11A2), protection from oxidative stress (e.g., UCP2, PARK7), and NRBC enucleation (e.g., ID2, RB1). Interestingly, genes involved in apoptosis (e.g., CASP8, P2RX1) and immune response (e.g., FOXO3, TRAF6) were also upregulated in the last stage (stage 3) of maturation of NRBC precursors. Our results confirm and extend on previous observations and providing a frame of reference for better understanding the critical steps of human erythroid maturation and its potential alteration in patients with different clonal and non-clonal erythropoietic disorders.
- Published
- 2019
49. IMAGe SYNDROME: A COMPLEX DISORDER AFFECTING GROWTH, ADRENAL AND GONADAL FUNCTION, AND SKELETAL DEVELOPMENT
- Author
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PEDREIRA, C. C., SAVARIRAYAN, R., and ZACHARIN, MARGARET R.
- Published
- 2004
50. Optimal schedule for cancer chemotherapy
- Author
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Pedreira, C. E. and Vila, V. B.
- Published
- 1991
- Full Text
- View/download PDF
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