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1. Embryo Development of Tree Frog Polypedates leucomystax at Campus of State University of Malang

Author

Pearlindah, Emy Kusumawati, Dian Ratri Wulandari, and Dwi Listyorini

Subjects
embryo development, Polypedates leucomystax, Tree frog conservation, Biology (General), QH301-705.5
Abstract

Tree frogs live in natural places which are unpolluted. Regarding their role as an ecological indicator, the decrease of frogs population in a particular habitat indicates the danger of environment quality decrease. Moreover, this condition can harm the frogs themselves. All kinds of frogs breed in aqueous environment such as ponds, marshes, and farming fields. One of the tree frogs, Polypedates leucomystax, which belongs to Familia Rachophoridae, is widely spread in Indonesia. This frog has yellowish brown skin with black spots or six lines extending from head to the posterior tip of body. A breeding couple of the frog produces foam nests on the water or plants around water body, where they will nest their fertilized eggs. This species produces over a hundred embryos in one spawning season. These embryos require appropriate conditions to develop normally in the nature. Frog embryo development may becomes a reference to understand how the frog population survives. This study focused on P. leucomystax with regards to its decrease in number due to the drying up of the environment and a lot lost of trees in Campus of State University of Malang. The development of P. leucomystax embryos in the reproduction foam was observed until it reached a tadpole stage. The result showed that the embryos developed in the foam until they hatched then they move out of the foam into the water under which they would continue their development. Considering that water body is a critical requirement for the development of P. leucomystax embryos, it is our responsibility to make any efforts to conserve not only the trees but also any type of water bodies including ponds, marshes, and farming fields as well.

Published
2012

2. Embryo Development of Tree Frog Polypedates leucomystax at Campus of State University of Malang

Author

Dian Ratri Wulandari, Emy Kusumawati, Dwi Listyorini, and Pearlindah Peralindah

Subjects
education.field_of_study, geography, Marsh, geography.geographical_feature_category, Ecology, Bubble nest, Population, Polypedates leucomystax, Tree frog, embryo development, Plant Science, Biology, biology.organism_classification, Tadpole, Nest, lcsh:Biology (General), Insect Science, Tree frog conservation, education, Molecular Biology, lcsh:QH301-705.5, Ecology, Evolution, Behavior and Systematics, Black spot
Abstract

Tree frogs live in natural places which are unpolluted. Regarding their role as an ecological indicator, the decrease of frogs population in a particular habitat indicates the danger of environment quality decrease. Moreover, this condition can harm the frogs themselves. All kinds of frogs breed in aqueous environment such as ponds, marshes, and farming fields. One of the tree frogs, Polypedates leucomystax , which belongs to Familia Rachophoridae, is widely spread in Indonesia. This frog has yellowish brown skin with black spots or six lines extending from head to the posterior tip of body. A breeding couple of the frog produces foam nests on the water or plants around water body, where they will nest their fertilized eggs. This species produces over a hundred embryos in one spawning season. These embryos require appropriate conditions to develop normally in the nature. Frog embryo development may becomes a reference to understand how the frog population survives. This study focused on P. leucomystax with regards to its decrease in number due to the drying up of the environment and a lot lost of trees in Campus of State University of Malang. The development of P. leucomystax embryos in the reproduction foam was observed until it reached a tadpole stage. The result showed that the embryos developed in the foam until they hatched then they move out of the foam into the water under which they would continue their development. Considering that water body is a critical requirement for the development of P. leucomystax embryos, it is our responsibility to make any efforts to conserve not only the trees but also any type of water bodies including ponds, marshes, and farming fields as well. Keywords: embryo development, Polypedates leucomystax, Tree frog conservation

Published
2012

3. Optimasi isolasi RNA jaringan kanker payudara terfiksasi formalin dari penderita kanker payudara di Rumah Sakit Saiful Anwar Malang dengan Kit Isolasi RNA Geneaid / Pearlindah

Author

Pearlindah and Pearlindah

Abstract

Pearlindah 2014. Optimasi Isolasi RNA Jaringan Kanker Payudara Terfiksasi Formalin dari Penderita Kanker Payudara di Rumah Sakit Saiful Anwar Malang dengan Kit Isolasi RNA Geneaid. Skripsi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang. Pembimbing (I) Dra. Dwi Listyorini M.Si D.Sc (II) Dr. H. Abdul Gofur M.Si. Kata Kunci isolasi rna jaringan kanker payudara terfiksasi formalin penderita kanker payudara di rumah sakit saiful anwar Malang 12288 12288 12288 12288 Isolat RNA total yang didapat dari jaringan kanker yang difiksasi pada umumnya berkualitas rendah. Optimasi untuk mendapatkan isolat RNA total yang berasal dari jaringan terfiksasi formalin dengan kualitas yang tinggi sangat diperlukan. Penelitian ini betujuan untuk mendapatkan protokol yang optimum dengan mengukur dari tingkat rasio kemurnian A260/280 yang menggunakan Nanodrop ND 2000 Spektrofotometer. 12288 12288 12288 12288 Modifikasi protokol yang dilakukan diantaranya menambah banyaknya etanol 70% dalam DEPC treated water dan lisis buffer (RB buffer) menambah reagen lain seperti Proteinase K mengubah durasi inkubasi tahap elusi RNA dan penambahan Proteinase K mengubah durasi sentrifugasi tahap lisis sel dan pencucian serta mengubah kecepatan sentrifugasi. Berbagai macam modifikasi protokol isolasi total RNA yang telah dilakukan dengan melihat tingkat kemurniannya ditunjukkan pada penambahan Proteinase K dan tingkat kecepatan sentrifugasi mendapatkan hasil yang murni dan stabil dengan penambahan Proteinase K sebanyak 30 l lama inkubasi selama 30 menit pada suhu 60oC serta tingkat kecepatan sentrifugasi 15.500 x g atau 16.000 x g. Hal ini telah diketahui bahwa Proteinase K berguna untuk penghancuran protein dalam lisat sel (jaringan kultur sel) dan pelepasan asam nukleat karena sangat efektif menonaktifkan DNase dan RNase. Hasil modifikasi protokol dengan kualitas yang baik antara 1.8-2.1 yaitu dengan penambahan Proteinase K dan sentrifugasi 15.500 x g dan 16.000 x g

Published
2014

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