1. A Multiomic Approach to Understand How Pleurotus eryngii Transforms Non-Woody Lignocellulosic Material.
- Author
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Peña, Ander, Babiker, Rashid, Chaduli, Delphine, Lipzen, Anna, Wang, Mei, Chovatia, Mansi, Rencoret, Jorge, Marques, Gisela, Sánchez-Ruiz, María Isabel, Kijpornyongpan, Teeratas, Salvachúa, Davinia, Camarero, Susana, Ng, Vivian, Gutiérrez, Ana, Grigoriev, Igor V, Rosso, Marie-Noëlle, Martínez, Angel T, and Ruiz-Dueñas, Francisco J
- Subjects
Pleurotus eryngii ,carbohydrate-active enzymes ,lignin-modifying enzymes ,lignocellulose transformation ,metabolomics ,oxidoreductases ,proteomics ,solid-state fermentation ,transcriptomics ,wheat–straw ,wheat-straw - Abstract
Pleurotus eryngii is a grassland-inhabiting fungus of biotechnological interest due to its ability to colonize non-woody lignocellulosic material. Genomic, transcriptomic, exoproteomic, and metabolomic analyses were combined to explain the enzymatic aspects underlaying wheat-straw transformation. Up-regulated and constitutive glycoside-hydrolases, polysaccharide-lyases, and carbohydrate-esterases active on polysaccharides, laccases active on lignin, and a surprisingly high amount of constitutive/inducible aryl-alcohol oxidases (AAOs) constituted the suite of extracellular enzymes at early fungal growth. Higher enzyme diversity and abundance characterized the longer-term growth, with an array of oxidoreductases involved in depolymerization of both cellulose and lignin, which were often up-regulated since initial growth. These oxidative enzymes included lytic polysaccharide monooxygenases (LPMOs) acting on crystalline polysaccharides, cellobiose dehydrogenase involved in LPMO activation, and ligninolytic peroxidases (mainly manganese-oxidizing peroxidases), together with highly abundant H2O2-producing AAOs. Interestingly, some of the most relevant enzymes acting on polysaccharides were appended to a cellulose-binding module. This is potentially related to the non-woody habitat of P. eryngii (in contrast to the wood habitat of many basidiomycetes). Additionally, insights into the intracellular catabolism of aromatic compounds, which is a neglected area of study in lignin degradation by basidiomycetes, were also provided. The multiomic approach reveals that although non-woody decay does not result in dramatic modifications, as revealed by detailed 2D-NMR and other analyses, it implies activation of the complete set of hydrolytic and oxidative enzymes characterizing lignocellulose-decaying basidiomycetes.
- Published
- 2021