Search

The purpose of this study was to build a project-based learning (PjBL) model using Susan A. Ambrose et al. cycle's metacognition conceptual framework. The PjBL approach was created to improve the preservice science teacher's metacognitive skills. Methods: To modify the PjBL strategy, this study began with a basic review of PjBL and metacognitive skills backgrounds. Results: A panel of experts used the item-objective congruence index (IOC) at five distinct levels to evaluate the validity of the PjBL model. The PjBL model's appropriateness rating was calculated as 4.56, which showed that it was very suited.

The purposes of this research were to create project-based learning (PjBL) activities and to improve the students' 4Cs skills using PjBL. The 4Cs of the learning skills assessment test and a record of student behavior serve as research tools. The mean and percentage are the statistics that were employed in the study. The results demonstrate that: (1) students possess the 4Cs skills that passed the 70% criteria: communication skills (83.33%), critical thinking skills (70.83%), creativity skills (80.56%), and collaboration skills (85.42%). The examination of PjBL methodologies, with strongly suitable from three experts, includes step 1. Compose, step 2. Clarify, step 3. Comprehend, and step 4. Come Together.

In this study, Bacillus licheniformis BFP011 has been cultivated for producing antimicrobial compounds, which were concentrated from the crude supernatant and purified using thin-layer chromatography. The antimicrobial activity was investigated in the presence of several organic solvents and detergents, and inhibiting effects to various Gram-negative and Gram-positive human pathogenic and food spoilage bacteria were observed. Three bands (F4, F5 and F6) showing antimicrobial activity against Salmonella typhi ATCC 5784 were subjected to reversed-phase high-performance liquid chromatography purification. Two peaks of fraction F5, F5-P3 and F5-P4, showed 100% inhibition against S. typhi ATCC 5784. The contained mixture of antimicrobial compounds consists of macrolactins and amicoumacins, which induced the collapse and breaking of S. typhi ATCC 5784 cell membranes in a time-dependent manner. Taken collectively, the results indicate that these compounds may represent promising candidates for the development of food preservative agents of natural origin, as well as novel antimicrobial drug candidates against multiresistant bacterial strains. [ABSTRACT FROM AUTHOR]