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4 results on '"Payan, Brittany A."'

1. Gelatin maleimide microgels for hematopoietic progenitor cell encapsulation.

Author

Thompson, Gunnar B., Gilchrist, Aidan E., Lam, Vincent M., Nunes, Alison C., Payan, Brittany A., Mora‐Boza, Ana, Serrano, Julio F., García, Andrés J., and Harley, Brendan A. C.

Abstract

Hematopoietic stem cells (HSCs) are the apical cells of the hematopoietic system, giving rise to cells of the blood and lymph lineages. HSCs reside primarily within bone marrow niches that contain matrix and cell‐derived signals that help inform stem cell fate. Aspects of the bone marrow microenvironment have been captured in vitro by encapsulating cells within hydrogel matrices that mimic native mechanical and biochemical properties. Hydrogel microparticles, or microgels, are increasingly being used to assemble granular biomaterials for cell culture and noninvasive delivery applications. Here, we report the optimization of a gelatin maleimide hydrogel system to create monodisperse gelatin microgels via a flow‐focusing microfluidic process. We report characteristic hydrogel stiffness, stability, and swelling characteristics as well as encapsulation of murine hematopoietic stem and progenitor cells, and mesenchymal stem cells within microgels. Microgels support cell viability, confirming compatibility of the microfluidic encapsulation process with these sensitive bone marrow cell populations. Overall, this work presents a microgel‐based gelatin maleimide hydrogel as a foundation for future development of a multicellular artificial bone marrow culture system. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Epigallocatechin-3-gallate suppresses neutrophil migration speed in a transgenic zebrafish model accompanied by reduced inflammatory mediators

Author

Nguyen,Thao, Payan,Brittany, Zambrano,Amarayca, Du,Yong, Bondesson,Maria, and Mohan,Chandra

Subjects
Journal of Inflammation Research
Abstract

Thao Nguyen,1 Brittany Payan,1 Amarayca Zambrano,1 Yong Du,1 Maria Bondesson,2 Chandra Mohan11Biomedical Engineering Department, University of Houston, Houston, TX 77204, USA; 2Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN 47405, USACorrespondence: Chandra MohanDepartment of Biomedical Engineering, University of Houston, 3517 Cullen Blvd, Room 2004, Houston, TX 77204, USATel +1 713 743 3709Email cmohan@central.uh.eduBackground: Polyphenol catechins from green tea, particularly (−)-epigallocatechin-3-gallate (EGCG), exhibits numerous beneficial health effects, although the mechanisms remain unclear. Methods: In this study, the mechanism of EGCG-mediated healing in an experimentally injured zebrafish model was examined at the cellular and molecular level using confocal microscopy and gene expression analysis. Results: The mechanisms of action of EGCG were shown to involve: (1) reducing neutrophil response (accumulation, travel speed, and distance) and (2) downregulating the expression of IL-1β, TNFα, and related signaling pathways. As determined by dynamic time-lapse tracking studies, the local accumulation of neutrophils with high migration speeds after wounding (n=33 cells, v=0.020 μm/s, d=37.8 μm), underwent significant reduction following treatment with EGCG doses of 300 μM (n=22 cells, v=0.013 μm/s, d=39.5 μm) and 600 μM (n=18 cells, v=0.008 μm/s, d=9.53 μm). Reverse transcription polymerase chain reaction studies revealed that several signature genes in the IL-1β, TNFα, and related signaling pathways were downregulated after EGCG treatment. Conclusion: The convenience, transparency, and simplicity of the zebrafish model facilitate tracking of fluorescent neutrophils in real time, in order to monitor inflammation, and assess the impact of therapeutic agents.Keywords: green tea, innate immunity, animal models, IL-1, TNF

Published
2019

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